LOCUS       DAJTNW010000001        93034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNW010000001 DAJTNW010000000
VERSION     DAJTNW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 93034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO179_000001"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO179_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5530229.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO179_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO179_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5530230.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO179_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO179_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5530231.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO179_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO179_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5530232.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO179_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO179_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5530233.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO179_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO179_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5530234.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO179_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO179_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5530235.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO179_000008"
     CDS             complement(4598..5320)
                     /locus_tag="OO179_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5530236.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO179_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO179_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5530237.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO179_000010"
     CDS             6520..6738
                     /locus_tag="OO179_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5530238.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO179_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO179_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5530239.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO179_000012"
     CDS             complement(7472..7672)
                     /locus_tag="OO179_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5530240.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO179_000013"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO179_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO179_000014"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO179_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5530241.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO179_000015"
     CDS             10101..12014
                     /locus_tag="OO179_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5530242.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO179_000016"
     CDS             12014..13036
                     /locus_tag="OO179_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5530243.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO179_000017"
     CDS             13030..13248
                     /locus_tag="OO179_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5530244.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO179_000018"
     CDS             13302..14171
                     /locus_tag="OO179_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5530245.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO179_000019"
     CDS             complement(14226..14630)
                     /locus_tag="OO179_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5530246.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO179_000020"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO179_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5530247.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO179_000021"
     CDS             15615..17705
                     /locus_tag="OO179_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5530248.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO179_000022"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO179_000022"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5530249.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO179_000023"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO179_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5530250.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO179_000024"
     CDS             complement(19673..20275)
                     /locus_tag="OO179_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5530251.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO179_000025"
     CDS             complement(20265..20432)
                     /locus_tag="OO179_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5530252.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO179_000026"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO179_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5530253.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO179_000027"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO179_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5530254.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO179_000028"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO179_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5530255.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO179_000029"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO179_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5530256.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO179_000030"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO179_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5530257.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO179_000031"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO179_000031"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5530258.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO179_000032"
     CDS             28269..28436
                     /locus_tag="OO179_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5530259.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO179_000033"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO179_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO179_000034"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO179_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5530260.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO179_000035"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO179_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5530261.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO179_000036"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO179_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5530262.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO179_000037"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO179_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5530263.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO179_000038"
     CDS             complement(33764..34849)
                     /locus_tag="OO179_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5530264.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO179_000039"
     CDS             complement(34861..36165)
                     /locus_tag="OO179_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5530265.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO179_000040"
     CDS             complement(36177..36530)
                     /locus_tag="OO179_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5530266.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO179_000041"
     CDS             complement(36541..37419)
                     /locus_tag="OO179_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5530267.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO179_000042"
     CDS             complement(37416..38642)
                     /locus_tag="OO179_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5530268.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO179_000043"
     CDS             complement(38643..39806)
                     /locus_tag="OO179_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5530269.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39891..40253)
                     /locus_tag="OO179_000044"
     CDS             complement(39891..40253)
                     /locus_tag="OO179_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5530270.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40270..41175)
                     /locus_tag="OO179_000045"
     CDS             complement(40270..41175)
                     /locus_tag="OO179_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530271.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41445..42449)
                     /gene="trpS"
                     /locus_tag="OO179_000046"
     CDS             complement(41445..42449)
                     /gene="trpS"
                     /locus_tag="OO179_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5530272.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42442..43200)
                     /gene="gph"
                     /locus_tag="OO179_000047"
     CDS             complement(42442..43200)
                     /gene="gph"
                     /locus_tag="OO179_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5530273.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43193..43870)
                     /gene="rpe"
                     /locus_tag="OO179_000048"
     CDS             complement(43193..43870)
                     /gene="rpe"
                     /locus_tag="OO179_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5530274.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43888..44724)
                     /gene="dam"
                     /locus_tag="OO179_000049"
     CDS             complement(43888..44724)
                     /gene="dam"
                     /locus_tag="OO179_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5530275.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44831..46117)
                     /gene="damX"
                     /locus_tag="OO179_000050"
     CDS             complement(44831..46117)
                     /gene="damX"
                     /locus_tag="OO179_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5530276.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46209..47297)
                     /gene="aroB"
                     /locus_tag="OO179_000051"
     CDS             complement(46209..47297)
                     /gene="aroB"
                     /locus_tag="OO179_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5530277.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47354..47875)
                     /gene="aroK"
                     /locus_tag="OO179_000052"
     CDS             complement(47354..47875)
                     /gene="aroK"
                     /locus_tag="OO179_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5530278.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48276..49514)
                     /gene="hofQ"
                     /locus_tag="OO179_000053"
     CDS             complement(48276..49514)
                     /gene="hofQ"
                     /locus_tag="OO179_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5530279.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49426..49830)
                     /gene="hofP"
                     /locus_tag="OO179_000054"
     CDS             complement(49426..49830)
                     /gene="hofP"
                     /locus_tag="OO179_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5530280.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49820..50260)
                     /gene="hofO"
                     /locus_tag="OO179_000055"
     CDS             complement(49820..50260)
                     /gene="hofO"
                     /locus_tag="OO179_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5530281.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50244..50783)
                     /gene="hofN"
                     /locus_tag="OO179_000056"
     CDS             complement(50244..50783)
                     /gene="hofN"
                     /locus_tag="OO179_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5530282.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50783..51562)
                     /gene="hofM"
                     /locus_tag="OO179_000057"
     CDS             complement(50783..51562)
                     /gene="hofM"
                     /locus_tag="OO179_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5530283.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51682..54234
                     /gene="mrcA"
                     /locus_tag="OO179_000058"
     CDS             51682..54234
                     /gene="mrcA"
                     /locus_tag="OO179_000058"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5530284.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54399..54959)
                     /gene="nudE"
                     /locus_tag="OO179_000059"
     CDS             complement(54399..54959)
                     /gene="nudE"
                     /locus_tag="OO179_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5530285.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55279..57414
                     /gene="igaA"
                     /locus_tag="OO179_000060"
     CDS             55279..57414
                     /gene="igaA"
                     /locus_tag="OO179_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5530286.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEECEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57479..58147
                     /gene="yrfG"
                     /locus_tag="OO179_000061"
     CDS             57479..58147
                     /gene="yrfG"
                     /locus_tag="OO179_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5530287.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58158..58559
                     /gene="hslR"
                     /locus_tag="OO179_000062"
     CDS             58158..58559
                     /gene="hslR"
                     /locus_tag="OO179_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5530288.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58584..59453
                     /gene="hslO"
                     /locus_tag="OO179_000063"
     CDS             58584..59453
                     /gene="hslO"
                     /locus_tag="OO179_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5530289.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59597..61321)
                     /locus_tag="OO179_000064"
     CDS             complement(59597..61321)
                     /locus_tag="OO179_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5530290.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61700..63322
                     /gene="pckA"
                     /locus_tag="OO179_000065"
     CDS             61700..63322
                     /gene="pckA"
                     /locus_tag="OO179_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5530291.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63398..64750)
                     /gene="envZ"
                     /locus_tag="OO179_000066"
     CDS             complement(63398..64750)
                     /gene="envZ"
                     /locus_tag="OO179_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5530292.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64747..65466)
                     /gene="ompR"
                     /locus_tag="OO179_000067"
     CDS             complement(64747..65466)
                     /gene="ompR"
                     /locus_tag="OO179_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5530293.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65694..66170
                     /gene="greB"
                     /locus_tag="OO179_000068"
     CDS             65694..66170
                     /gene="greB"
                     /locus_tag="OO179_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5530294.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66267..68588
                     /locus_tag="OO179_000069"
     CDS             66267..68588
                     /locus_tag="OO179_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5530295.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69026..69253
                     /gene="feoA"
                     /locus_tag="OO179_000070"
     CDS             69026..69253
                     /gene="feoA"
                     /locus_tag="OO179_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5530296.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69270..71591
                     /gene="feoB"
                     /locus_tag="OO179_000071"
     CDS             69270..71591
                     /gene="feoB"
                     /locus_tag="OO179_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5530297.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71591..71827
                     /gene="feoC"
                     /locus_tag="OO179_000072"
     CDS             71591..71827
                     /gene="feoC"
                     /locus_tag="OO179_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5530298.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72030..72908
                     /gene="rpnA"
                     /locus_tag="OO179_000073"
     CDS             72030..72908
                     /gene="rpnA"
                     /locus_tag="OO179_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5530299.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            72981..73250
                     /locus_tag="OO179_000074"
     CDS             72981..73250
                     /locus_tag="OO179_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5530300.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73244..73609
                     /locus_tag="OO179_000075"
     CDS             73244..73609
                     /locus_tag="OO179_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5530301.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73610..74398)
                     /gene="bioH"
                     /locus_tag="OO179_000076"
     CDS             complement(73610..74398)
                     /gene="bioH"
                     /locus_tag="OO179_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5530302.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74436..75119
                     /gene="gntX"
                     /locus_tag="OO179_000077"
     CDS             74436..75119
                     /gene="gntX"
                     /locus_tag="OO179_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5530303.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75178..75753
                     /gene="nfuA"
                     /locus_tag="OO179_000078"
     CDS             75178..75753
                     /gene="nfuA"
                     /locus_tag="OO179_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5530304.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76114..77430
                     /gene="gntT"
                     /locus_tag="OO179_000079"
     CDS             76114..77430
                     /gene="gntT"
                     /locus_tag="OO179_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5530305.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77475..79559)
                     /gene="malQ"
                     /locus_tag="OO179_000080"
     CDS             complement(77475..79559)
                     /gene="malQ"
                     /locus_tag="OO179_000080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5530306.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79569..81962)
                     /gene="malP"
                     /locus_tag="OO179_000081"
     CDS             complement(79569..81962)
                     /gene="malP"
                     /locus_tag="OO179_000081"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5530307.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82574..85279
                     /gene="malT"
                     /locus_tag="OO179_000082"
                     /pseudo
     CDS             82574..85279
                     /gene="malT"
                     /locus_tag="OO179_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(85322..86338)
                     /gene="rtcA"
                     /locus_tag="OO179_000083"
     CDS             complement(85322..86338)
                     /gene="rtcA"
                     /locus_tag="OO179_000083"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5530308.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(86342..87568)
                     /gene="rtcB"
                     /locus_tag="OO179_000084"
     CDS             complement(86342..87568)
                     /gene="rtcB"
                     /locus_tag="OO179_000084"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5530309.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87757..89355
                     /gene="rtcR"
                     /locus_tag="OO179_000085"
     CDS             87757..89355
                     /gene="rtcR"
                     /locus_tag="OO179_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5530310.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(89337..90095)
                     /locus_tag="OO179_000086"
     CDS             complement(89337..90095)
                     /locus_tag="OO179_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5530311.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(90112..90942)
                     /gene="glpG"
                     /locus_tag="OO179_000087"
     CDS             complement(90112..90942)
                     /gene="glpG"
                     /locus_tag="OO179_000087"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5530312.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90987..91313)
                     /gene="glpE"
                     /locus_tag="OO179_000088"
     CDS             complement(90987..91313)
                     /gene="glpE"
                     /locus_tag="OO179_000088"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5530313.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            91503..93008
                     /gene="glpD"
                     /locus_tag="OO179_000089"
     CDS             91503..93008
                     /gene="glpD"
                     /locus_tag="OO179_000089"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5530314.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT        22282 a        25300 c        23551 g        21901 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcttgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 gactgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgcccccc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt ttgaatattt aggttgtgcg acgtggattt gtcgggagct tcaaacctct
    39901 tcgttagcag ccatccataa tccataaaga ttggccagaa aatagccttc ttcacacggg
    39961 ttaacttcca gagcgaattc cttcaacaac tcctgatgca attgcacaac ggcttgccag
    40021 tggctggatt gcgccagttc cgccagcaac tcgttatcca gcggctctat ttcttcacca
    40081 cggcgactgc gcatcagtgc actcgccata tgtgtcatcg ccatcgttcc ttgctcactg
    40141 cgcaccggca gatggcactc tttttccagt acgttgacga cctgcatcat gcctttgcag
    40201 acgtccttat caataacgcc tgcctcgcaa agcaggttga gtctggtttc catatcagat
    40261 gcctttcgtt taataactgg gttcctgttc cccactcacc acccgttgtt gatgggcaag
    40321 cagggcgtgt ttatctacaa cagcacgcag caattgttgc attgggtaat catcgactcg
    40381 cgtcaggacc acggcttcgg tggcctgtaa aaatcgcgga tcgtctgtta gcggcgtcgc
    40441 ctctaatccc agcatggtca gttcgttttc cgccaccacg ttccagatca ccgcatcgac
    40501 atcgccttta acaatgcgtt gtaaactctc gtgataagag agatcgactc gttccacatc
    40561 actatcgcca aaaaagacat cggtcatgat tttctgatcc gccgaacgac tatcgagacc
    40621 tactcgctta acatttccag actcaccttt gcggcatatc agttgatgtt caccaacata
    40681 cgtatgcggg ccaagctcga gggcgatgca caaattgttt tggctgagat aactttccgc
    40741 cgccaggcgg gaaactactg ccatgtcata gacaccgtta agtaaacact ccacgcgaat
    40801 atccgcgccg cgcatatgcg catagtaaaa aggaatgcca tcaaactggg ctttcaatcc
    40861 gctcgctaaa ccttcgtaca agcgggtata gggcaaaggc atcgcacata ccacgttgtt
    40921 gatatccaca tgactcagca atgctttgtt atccatctcg acaagataac tgccattgcg
    40981 cccacggcgt tcaatccgta tcgctccgct tgattccagc gttttcaacg cggcctgcgt
    41041 caggccaacc gatgaacggc attcagttgc cagctcatct atggttttca gtcgattacc
    41101 gcatttttca cccaacaaat aacgggccag tgtcgtgatg acgacgcctt cttttttgat
    41161 aaacgttcga cgcataatat attttcaata aaatgaatat ttatatcttc attaaaatga
    41221 agataagtgg cgacgacgag atgtggaaac ggcgaggcgc ttcacatttt ttcgggttgt
    41281 tgcgaaaaga ggataaaaaa accgggtttc cccggtttca gagtgatgac gaagtgattt
    41341 attcgacaat gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa
    41401 ccctctcccc aaaggggcga ggggaccgtc cgagcgtttt gatattacgg cttcgccaca
    41461 aaaccaatcg cttcgtacac cgctttcagc gtacgggaag cgtgtacact ggctttttct
    41521 gcgccgtctt tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt
    41581 tcctgcaatt cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca
    41641 tacatcttgc cttcgaactg tttttccagt tctgggatgc tctggcccgt taccgcagaa
    41701 aggatatcca gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc
    41761 ggctcgtcgg agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc
    41821 agcaggccga taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc
    41881 agcagcgaca ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc
    41941 tcgccataca gcgcgttgaa acgctgggcg atatcgcgac tcagttccag gtgctgtttc
    42001 tggtcttcac ccaccggtac cagattagtt tgatacagca ggatgtccgc agccatcagc
    42061 accggatagc caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta
    42121 aactgcgtca tgcgactcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac
    42181 tgcgcatgtt ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca
    42241 caagccagat acaaggccag cgtatccaac gtcgctttac gcagcttctg tgcatcctgg
    42301 cgcacggtga tcgcgtgttg gtcaacgata cagtaaacgc aatggtagtc atcctgcatg
    42361 tttacccact gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt
    42421 gcgccactaa aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag
    42481 ccctaatgcg ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc
    42541 gatagcctcg ccgtagttgt atccgtaggt taagccaact gatgggcaac ctgccgcttt
    42601 tgccgcctga atatcattcc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc
    42661 catccgctca gccaccagta acagcgggtc cggatgcggt tttttgtttt gcacatcatc
    42721 gccgccgata accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac
    42781 gaacggcgtc ggtttgttgg tgaccaagcc tagcggcagg cctttagcct gcaacgcgcc
    42841 caacgtatcg gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgccatagta
    42901 gcgatcgaac agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac
    42961 gggcggtttc cccattgttt tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc
    43021 gcgctccatc agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc
    43081 gacgggcaac tccagcgcat acagcgccat atctaccgca gcagcaagac caggagcact
    43141 gtcgaccagc gtaccatcaa gatcaaaagc gacgccgcga atatcttcaa acttattcat
    43201 gacttacctt tgccagttca ctgcgcattt catcaatgac ttttttatag tctggctggt
    43261 cgaagattgc cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt
    43321 tgttcacctt cacgccaccg tccacttcca gacgaatgtc aaagccagac tcgtcgatac
    43381 ggcgacgtac ttcgcgcagt ttatccagtg tttgaggaat gaaagactga ccgccgaaac
    43441 cagggttgac ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagatagc
    43501 tcagaggtgt cgccgggtta aataccagac ccgctttaca gccattttct ttaatcagtt
    43561 gcagcgtgcg gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag
    43621 cggcagcgaa atcaggcaca atgcgatcga cgggtttcac catcagatgt acgtcgatag
    43681 gggcggtaat gccgtagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg
    43741 gaacatagtg gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt
    43801 ttgcggtatc ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat
    43861 actgtttcat ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt
    43921 gtacaaagcc agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg
    43981 tcgaactttg acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc
    44041 gtgattggag atcagcactg gaatatggcg ctcaaccaga ccttcggcga tctccgccag
    44101 atgcgcttgt tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc
    44161 agacagcggt gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgctccat
    44221 gctatcggcg taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata
    44281 caactctgct tccgggaaat agggtttttt gtagcggcca aacggcacgt taaactcacc
    44341 gcgcagattg taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac
    44401 cgcccgacgg aacggatcct ggcttttgtt gaactcttcg cggaactgat agtaaacctc
    44461 ggcgcaattt gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg
    44521 catcttcaca atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg
    44581 agaaaagtcg gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc
    44641 gcccttgggc aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt
    44701 caaaaaagcg cgatttttct tcatgccgat taactaatta caccttctcc ggctgtggag
    44761 aaagctccga cagcatccag cgcttcaggc agctcccgca acgataaacc attgcgggag
    44821 ataaccttga ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt
    44881 tggcctgaac atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt
    44941 acacgccaga aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt
    45001 agtttttcag attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc
    45061 tcagctgcag agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg
    45121 tcttccctcc agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag
    45181 ccgtagtcgc cgtctctttt ggtgctggcg tagaagtcgc agccggtgcc ttcgtgctcg
    45241 ccacaggggc agctggttca gtacgcttcg gcgtctgtgc taccggcttc ggctccgttt
    45301 tcacggttgc ttgcggtttt ttcggttcaa tcaccgcctg ctgacgaact gggcgcgtag
    45361 tggacggacg ttcagcggtt tgcgttttcg ccgagtcacg cgatgcattg ccattgcgaa
    45421 caggcgcaac cgttgcgggt tcagtcggca acgtggaatt gaccgccaca ttgttcagct
    45481 gttgctgatt ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt
    45541 gttgaccatc cgttgccacc ggggtttgcc cttgagtcgg cgtagaagag atcggtggca
    45601 gcgaaacatc ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat
    45661 tcgcctgatc ggtcgcattg ccagcaagat caatactctt ctcgccagac gcggtttgat
    45721 cgctggaagt ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta
    45781 gaaccagaat gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt
    45841 ttttgcgctt acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt
    45901 cgtcaatttc ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac
    45961 ggcgatcgtc agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt
    46021 cagtacgctc agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat
    46081 cgggtttcag ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa
    46141 tgcttaccac gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt
    46201 tcttgttgtt acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg
    46261 ccgctgcgaa cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca
    46321 aggactttct tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc
    46381 ccattgaccg gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa
    46441 ctaaactgcc cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct
    46501 tcaccatgta accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt
    46561 cccagattca gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc
    46621 ttcagttcac aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac
    46681 gcatccagat tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg
    46741 atgacttctg ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga
    46801 tccaccacca ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg
    46861 ttgaccgcag ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact
    46921 tgaatgaaac gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact
    46981 acgccgccgc caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc
    47041 gtaaagacgg tatcgagtac agctaggctt ttatactgct cgccgtcagg gaggataacg
    47101 ctatcgacgt taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga
    47161 gccagggttt cgctggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa
    47221 gctggttcat taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta
    47281 acgacaatcc tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta
    47341 tataaagcca gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag
    47401 cgctttgatc atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt
    47461 cattggccaa cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt
    47521 ttttatcacg ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa
    47581 cgccacgagc ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag
    47641 ccagcacaat accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga
    47701 agccttcttc gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa
    47761 tctcttgatc ggaatcgtaa aattccatat tgagctgttg agctaactgg cgcccaatag
    47821 tgctttttcc ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt
    47881 cggtactact aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga
    47941 catgaactga aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc
    48001 agcgggtttg gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc
    48061 gctgccttgc taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac
    48121 agcaaaatgc cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca
    48181 actctcaaat cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac
    48241 gttcagcata aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc
    48301 gtgataaaca ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc
    48361 ccgaaccagg gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg
    48421 gtaaaaatgc cgcccagcgc caacgtttct ccgcttttta cctcgacctg cgtttcgatc
    48481 tcctgcttat caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg
    48541 ttctggctga tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc
    48601 tccatcccca ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa
    48661 acctgatatg gaatttcgct ctcctgttta atgctggcag gctgaagatg tgaggccagc
    48721 agacgcggac tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga
    48781 tccagcaagc gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct
    48841 acggagaggt cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg
    48901 gccagcgtcc atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc
    48961 gccgacagct caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg
    49021 cttaacgccg ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata
    49081 ctccctttgg cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat
    49141 tgcagggtta tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc
    49201 tcctggcggg cgatattgtc attctgccag gcaatggaat gcactgagag aatgttgcct
    49261 tcctgccgcg ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga
    49321 acatctgtta gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac
    49381 ttctcctgtt cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc
    49441 gtcacttttt gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct
    49501 atccattgct tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt
    49561 tgtcccggta cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc agccgttttc
    49621 cagcacatcg ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat
    49681 gatgcgctcg cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg
    49741 gcatagatct tccggcgatt taaaagggtc acgcataccg gttaaaaggc acaatgcaat
    49801 acctgccaac aaccagcgtt taaccctcat gcggcatctc cagttgtagc gtgaacaaaa
    49861 gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc
    49921 gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactcg cctccctgcg
    49981 cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt ggcgaaaagg
    50041 gcagcgtttt ttcctcgctg aagggagtgg tgtctaccag gcgatacagt gcggcccact
    50101 gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg
    50161 atagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac tggcggaggc
    50221 ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac
    50281 tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg ctgattgaga
    50341 tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt
    50401 gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcgtgc
    50461 tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc
    50521 tggcgctgcc gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa acgtggcttc
    50581 gttatctgta agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc gtctatgcgc
    50641 gcttcggcgc tgcctgtcag acgcagtatt agcgttatcc cgacggccag cagcagaggc
    50701 gcaacgaaca tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc
    50761 aaaaaattaa ttggcgggtt catcagtacg ccttcccaag cgccagcccc agcgcgatgg
    50821 caaagtctcc accaggcggc ggtagcggcg gctgacgaac agaaacggcc tcccaggggt
    50881 caaatccgcc ttcgccacat atcgcgacgc tgtcgggatc aacggataac gctgccgcca
    50941 gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag cgcgtcgccc
    51001 acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca
    51061 ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca cgcaaccttt
    51121 ctgccagcgt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataagcgg
    51181 ggctgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata tccagctcgc
    51241 gggccatcgt ccctgacagc caggccgttt gctcccgctc accaagggac atcgacgggc
    51301 gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct
    51361 gcggcagttc gcgactccac ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc
    51421 gcccatcttt gataatgtcg tgcgccagcg gcaaccgcca ccagcgttgc aaaaagcatt
    51481 cttttgcgcc ccgcacgatc gcaaccgctg ccgcttcttg ctgttgtaaa tgcaaaccaa
    51541 tttgccagat cttaaatgtc attgtgatga tctccttatc acccgtcact ctgacgggta
    51601 tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg
    51661 aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt
    51721 tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg
    51781 ccggatgtgg tgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc
    51841 gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa
    51901 atcccaccgg agatggtgaa agcctttatc gcgacggaag acagccgctt ctacgagcat
    51961 cacggagttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt ctccggccac
    52021 gcgtcacaag gggcgagtac cattacccag cagctggcga gaaatttctt cctcagtcca
    52081 gaacgcacga tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat tgaacagttg
    52141 ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttaccttgg ttaccgcgcc
    52201 tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca actgacgctg
    52261 aacgaaatgg cggtgatagc cgggttgccg aaagcgcctt ccacctttaa tccgctctac
    52321 tcaatggatc gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct ggatgaaggg
    52381 tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc taattatcac
    52441 gcgccggaga ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat
    52501 aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc
    52561 aaagtgcagc aggccgcgca gcaggcggta cgtaataacg tgctggacta cgacatgcgc
    52621 cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac
    52681 aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct gcctgccgca
    52741 gtcaccagcg ccaatcctca gggagcgacg gcgatgctgg cggacgggtc gaccgtcgca
    52801 ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca gcagggaccg
    52861 acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt
    52921 ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt gtcgatcaat
    52981 ccgcaaaatg gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt
    53041 aaccgcgcca cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc
    53101 gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttctcgc
    53161 tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca gtatgctggt
    53221 ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgtgcaatg
    53281 cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcgcaa
    53341 aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg
    53401 gcgcgcggct acgcggtcat ggcgaacggc ggcttcctag tggacccgtg gtttatcagc
    53461 aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg
    53521 gaatgcgata ttccagtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac
    53581 gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag
    53641 ctggagcagg caaatcaggc gttagtggcg aagactggcg cgcaggagta cgcaccgcac
    53701 gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa tatctttggt
    53761 gaaccaggct ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc
    53821 ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg
    53881 ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca
    53941 acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc caagagtgcc
    54001 cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg
    54061 acgccgcctc cgggtattgt gacggtgaat atcgatcgca gcaccgggca gttagctaat
    54121 ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaggcggtg
    54181 cacgaggtgg gaacgaccat tatcgataat ggtgaggcac aggaattgtt ctgattaaaa
    54241 aggcgcttcg gcgccttttc agttggctga caaagtgcat ttgtttatgc cggatgcggg
    54301 tgaacgcgtt atccggctaa caaaatcgtg aaaaatcaat atattgcagg aaccccataa
    54361 gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc ctgccctttc
    54421 aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa gtcagggtct
    54481 tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtagcgg ctctggctca
    54541 tcgccttcca gcgattccgg gtagagatct tgcgcgacca cgatattcat tttgctggaa
    54601 aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc accaaatcca
    54661 acctcttctt ttagctcgcg gttagcggct tcgtagacgc tttcacccgg atcaattaat
    54721 cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg gatcaggatc
    54781 aggtgatcat ccacaatcgg cacaatcatc actgcttccc ggttggttgg acgcattcgt
    54841 tcataaacac gccgcacgcc attgctgaac tccagatcca cgctctcgac ggtaaacagt
    54901 cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga tttgttcatc
    54961 gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc tttcggcaat
    55021 gtgaattgca tgttatttac atttatgtaa cttaataaat aatcgtcctc aaatcaaatt
    55081 aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt cagagggttt
    55141 gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaagct ccacgcttgc
    55201 cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc ctgacagact
    55261 aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt gctggcctgc
    55321 tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct gccctggacc
    55381 aacgccttcg cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag cgccgttgaa
    55441 aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacggg agctagcgcg
    55501 gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct gacacacgct
    55561 atcacacgtt acggcatctc taccgacgat ccgaataaat ggcgttacta cctcgattcg
    55621 gtagaagtcc acctgccccc cttctgggaa cagtacatca acgatgagaa taccgttgaa
    55681 ctgattcata ccgattcgct gccgttggtt atttcactca acggtcatac gctgcaggag
    55741 tacatgcagg aaacgcgcgg ctatgccttg caacctgttc cgtccacgca ggcgtcgatt
    55801 cgcggggaag agtgtgagca aatcgagcta ctcaatattc gcaaagaaac gcatgaagaa
    55861 tatgcgctga gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc ctccttcctg
    55921 atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc aggcggcgca
    55981 ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg
    56041 cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat
    56101 caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg
    56161 cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta tctcgaccgc
    56221 cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta
    56281 cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctggtgct gtttatgctg
    56341 ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat gaaaggcgcg
    56401 cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg ggtaggcgat
    56461 acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac ctggagcgcg
    56521 aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa tgacgcccgc
    56581 tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac tgaagcagtt
    56641 aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attgcgttca
    56701 gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt actgaagaca
    56761 gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc
    56821 ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa gctcgatggc
    56881 gtgaatgtgt tattacgccc ggtgagtgcg gaatcgctgg ataacctggt ggcaacctcc
    56941 accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc
    57001 cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct
    57061 tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca gaaactggca
    57121 caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac
    57181 gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg cctgtggcgc
    57241 tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta caatggcgta
    57301 caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca ggcctattat
    57361 gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt
    57421 tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat
    57481 gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacgtt
    57541 gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc
    57601 gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt
    57661 acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat
    57721 ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata ccattccgtt
    57781 tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg ctcatccgca
    57841 caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt tattactttc
    57901 cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc
    57961 tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc
    58021 tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt ccgggattgc
    58081 cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct
    58141 aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg gataaatggc
    58201 tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta
    58261 aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc
    58321 tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc
    58381 gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac
    58441 gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac ccggaccgac
    58501 gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac
    58561 tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg
    58621 aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg
    58681 agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg gttgcgacca
    58741 gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg cagggcgacg
    58801 gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc ggtgtggcgc
    58861 gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg
    58921 tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt ggtctggaag
    58981 gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag ctgccgacgc
    59041 gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc
    59101 aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg acgctaaccg
    59161 aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt
    59221 atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct
    59281 cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt gatagcatcc
    59341 tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca
    59401 atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg taagttcatt
    59461 aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg cctgatgtgc
    59521 tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata cagcgtcgta
    59581 tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca tctaccgtca
    59641 gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct gtgagtaatt
    59701 tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt
    59761 ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg atccgctccg
    59821 cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt tcaatacagg
    59881 tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag
    59941 aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt tcctgaagat
    60001 gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct ttcataaacc
    60061 cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc tgttcgagca
    60121 tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt
    60181 tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt atcaccaaca
    60241 ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt tgcccattac
    60301 gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc tccgccagcg
    60361 cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacccccagg
    60421 tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg
    60481 gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt aagatcaatt
    60541 gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa atcgcgctca
    60601 ggcccgtaaa cggcagggtg atgataaaca tcggggttag caatgatact aacggcagca
    60661 accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac
    60721 gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata aaccagtcgg
    60781 ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac tcaccccaca
    60841 gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat ataagtacgt
    60901 tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg atacgaaggc
    60961 tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag ccaaaatccc
    61021 aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc agccaaccgc
    61081 tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa
    61141 aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa
    61201 caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa accagcccct
    61261 gaagtaatcc ggtagcaatc atcccccagc gtgttgccgg tgaaagttca acgttatcca
    61321 tgttggttat ccagaatcaa aaggtgggtt aattatcgca tccgggcagt agtattttgc
    61381 ttttttcaga aaataatcaa aaaaagttag cgtggtgaat cgatacttta ccggttgaat
    61441 ttgcatcaat ttcattcagg aatgcgattc cactcacaat attcccgcca tataaaccaa
    61501 gatttaacct tttgagaaca ttttccacac ctaaaatgct atttctgcga taatagcaac
    61561 cgtttcgtga caggaatcac ggagtttttt gtcaaatatg aatttctcca gatacgtaaa
    61621 tctatgagcc ttgtcgcggt taacaccccc aaaaagactt tactattcag gcaatacata
    61681 ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg cccccgcaag aactcgaggc
    61741 ttatggtatc agtgacgtac atgatatcgt ttacaaccca agctacgacc tgctgtatca
    61801 ggaagagctc gatccgagcc tgacaggtta tgagcgcggg gtgttaacta atctgggtgc
    61861 cgttgccgtc gataccggga tcttcaccgg tcgttcacca aaagataagt atatcgtccg
    61921 tgacgatacc actcgcgata ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa
    61981 caaacctctc tctccggaaa cctggcagca tctgaaaggc ctggtgacca ggcagctttc
    62041 cggcaaacgt ctgttcgttg tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc
    62101 cgtccgtttc atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg
    62161 cccgagcgat gaagagctgg caggtttcaa accagacttt atcgttatga acggcgcgaa
    62221 gtgcactaac ccgcagtgga aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa
    62281 cctgaccgag cgcatgcagc tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg
    62341 gatgttctcg atgatgaact acctgctgcc gctgaaaggt atcgcttcta tgcactgctc
    62401 cgccaacgtt ggcgagaaag gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa
    62461 aaccaccctt tccaccgacc cgaaacgccg cctgattggc gatgacgaac acggctggga
    62521 cgatgacggc gtgtttaatt tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa
    62581 agaagcggaa cctgaaatct acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac
    62641 cgtgcgtgaa gatggcacta tcgactttga tgatggttca aaaaccgaga acacccgcgt
    62701 ttcttatcca atctatcaca tcgataacat cgttaagccg gtttccaaag cgggccacgc
    62761 gactaaggtt atcttcctga ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct
    62821 gactgccgat caaacccagt atcacttcct ctctggcttc accgccaaac tggccggtac
    62881 tgagcgtggc atcaccgaac cgacgccaac cttctccgct tgcttcggcg cggcgttcct
    62941 gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc
    63001 gcaggcttat ctggttaaca ctggctggaa cggcactggc aaacgtatct cgattaaaga
    63061 tacccgcgcc attatcgacg ccatcctcaa cggttcgctg gataatgcag aaaccttcac
    63121 tctgccgatg tttaacctgg cgatcccaac cgaactgccg ggcgtagaca cgaagattct
    63181 cgatccgcgt aacacctacg cttctccgga acagtggcag gaaaaagccg aaaccctggc
    63241 gaaactgttt atcgacaact tcgataaata caccgacacc cctgcgggtg ccgcgctggt
    63301 agcggctggt ccgaaactgt aatgatttga agctggagaa tatctatcca gtatcttata
    63361 gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta cccttctttt gtcgtgccct
    63421 gcgcccgcgt taccggcact ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg
    63481 tgccaagctc cagcatcccg ttatggttat ccacgatacg ctgcacaatt gccagcccta
    63541 atcccgtgcc gctaatggtg cgcgcactgt cgccgcggac aaacggctgg aacaggtgct
    63601 tacgttgttc cggcgcaatt cccggaccgt catcttccac ctggaaccag gcgcgattcg
    63661 gctccgttcc gctgctgact ttgatccagc cattgccata acgggcggcg ttgaccacca
    63721 tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg ctgccggggt
    63781 aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaatcacc tcaccgagca
    63841 ctgcattaag atccgccatt tccatcggca tctcctgccc ggtgcgcagg tagtcgataa
    63901 actgctcaat gatggcgttg cactcttcga tatctttatt gatcgattct gccagatagc
    63961 catcctgctc gctcatcatc tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt
    64021 cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta acaccagccg
    64081 ccatatggtt aaaggcacgg gtaacggaac gcacctccga agcgccatat tcacgcagcg
    64141 gcggcggaat aatcccttta ccaacctgca aggctgcgtg ttcgagatcg accaacggtc
    64201 ggttctggat acgaataaac agccacgccc cgcctatcgc caatagcata atcgccagcg
    64261 tatagcggaa cagcggagag aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc
    64321 agatattggg cgacagccag gttttcagcc agacgacagg cgaacttttg ttgacctcaa
    64381 cgcgcacttc cgtcgggccg cccagttgct gtgccatctg atggcttaag aattcatagt
    64441 gttgcgccca acgcagacct gcctcttcgg cagcctcgtt ggagtagaga gagatcccca
    64501 gctcacggta gatctcccga cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca
    64561 gttgcagttt gtcggtcatc aacatacgca cttcgtacgc gaggacttta ttaaactgct
    64621 ggaggctcgg caaaatcgcg aagttcagca ccaccagata agtcgtcacc aggctggcga
    64681 acagcaaggt gacgatgagc aataacgtac gggcaaatga acttcgtggc gagaagcgca
    64741 atcgcctcat gctttagagc cgtccggtac aaagacgtag cccaggcccc aaacggtctg
    64801 aatgtaacgc ggatgcgctg gatcttcttc taccatgcgg cgcagacgcg aaatctgcac
    64861 gtcgatggag cgttccattg cggaatattc acgaccacgg gcaaggttca tcagcttatc
    64921 gcgggagagc ggctcacgcg gatggctgac cagtgccttc agtaccgcaa actcaccgct
    64981 ggtgagcggc atcggctcat cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa
    65041 cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg
    65101 acgacgcagc accgcacgaa tacgggccag cagttcacgc gggttaaacg gttttggaat
    65161 gtagtcgtca gcgccaatct ccaggcctac gatacggtcc acttcttccc ctttcgccgt
    65221 caccataatg atcggcatcg ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc
    65281 atcttcacca ggtaacatta aatccagtac cataagatgg aaagattcac gagtcagcag
    65341 gcgatccatc tgttctgcat tagcgacgct tcgaacctgg aagccttgtt cggtgagata
    65401 acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc accagaatct tgtagttctc
    65461 ttgcattgtt tgtactccca aaggttcgca acaatttgta agcgtgtatt cttaaaaaag
    65521 ctcacgttcg tcaccagcta aatctggtat aaatttcagc ctaaattgtt acaaagcata
    65581 ttaaacagca gcttaagtat acaatttatt cggcgaaaca ttattgattc tgttgatatg
    65641 atcacgttat acccaatgtg cgcattatca aacagacaaa gggaatcaac gagatgaaaa
    65701 cgcccctggt tacccgggaa gggtatgaaa aactcaaaca agagcttaat tatctctggc
    65761 gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca
    65821 gcgaaaatgc tgactatcag tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc
    65881 gctatctcac taaatgcctg gaaaatctca aaatcgtcga ttactcccct cagcaggaag
    65941 gcaaagtatt ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt
    66001 tccgtattgt cggctacgat gaaatttttg gccgtaaaga ttacatctct atcgattccc
    66061 cgatggcccg cgcattgctg aaaaaagaag tcggcgatct ggcggtggtg aatacccctg
    66121 ccggggaagc gaactggtat gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct
    66181 taacctcctg ccgatggctg gcatttttgc cagccagtcc gtataactat cccctgattt
    66241 ttgatccgaa aagatgaact caaaccatga tgaatgattc gttctgccgc attattgcgg
    66301 gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt gacgaaggga
    66361 ataccgtgcc gtttatcgca cgttatcgta aggaaatcac cggcggtctg gatgacacgc
    66421 agctgcgtaa tctggaaacg cgtctgagct atctgcgcga gctggaagag agacgtcagg
    66481 cgatcctcaa atccatttcc gagcaaggca aactcaccga tgatctggcg aaggccatca
    66541 acgccaccct aagcaaaacc gaactcgaag acctctacct gccctacaaa cctaaacgcc
    66601 gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc gttggctgac ctgctgtgga
    66661 gcgatccgtc acacacgcca gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg
    66721 tggcagatac caaagccgcg ctggatggcg cgcgctatat cctgatggaa cggtttgccg
    66781 aagatgccgc gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg catttggttt
    66841 ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc
    66901 acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg
    66961 aaggcgtact ccagctttcg ctgaatgccg atccgcagtt cgatgagccg cccaaagaga
    67021 gctattgcga gcaaatcatc atggatcacc ttggcctgcg cctgaacaat gccccggcgg
    67081 atagctggcg caaaagcgta gtgagctgga cctggcgcat caaggtgctg atgcatctgg
    67141 aaaccgaact gatgggtacc gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg
    67201 cccgtaacct gcacgatctg ctgatggcgg cccctgccgg actgcgtgca acgatgggcc
    67261 tcgatccggg tctgcgtacc ggggtaaaag tggcggtggt cgatgccact ggcaaactgg
    67321 tggcgaccga taccatttac ccgcacaccg gacaagccgc aaaagcagcg atgaccgttg
    67381 ctgccttgtg tgaaaaacat aacgttgaac tggtggcgat cggcaacggt acagcttccc
    67441 gcgaaactga acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga
    67501 aagtgatcgt cagcgaagct ggcgcgtcgg tttactcagc ttccgagctg gcagcgcagg
    67561 agttcccgga tctcgacgtt tcgctgcgtg gtgcggtatc tatcgcccgc cgtttgcagg
    67621 atccgctggc ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc
    67681 atgacgtcag ccagacgcaa ctggcccgca aactggatgc agtagtagaa gactgcgtaa
    67741 acgccgttgg cgtcgatctc aacaccgctt ctgttccgct attaacccgc gtggcgggcc
    67801 tgacgcgcat gatggcgcaa aacatcgtgg cctggcgcga tgagaacggc cagttccaga
    67861 accgtcagca actgctgaaa gtcagccgtc tggggccgaa agccttcgag cagtgcgcgg
    67921 gcttcctgcg cattaaccac ggtgataacc cgctggacgc ctctaccgtt catccggaag
    67981 cctatccggt ggtggaacgc attctggcag caacacagca ggcactgaaa gatctgatgg
    68041 gtaacagcag cgaactgcgt aacctgaaag cgtctgactt tactgatgaa aaattcggtg
    68101 tgccgacagt aactgacatc atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg
    68161 aatttaaaac cgctcagttt gccgatggcg tcgagacaat gaacgacctg caaccgggta
    68221 tgatcctcga aggcgcagtg accaacgtca ccaactttgg cgcgtttgtc gatattggcg
    68281 tgcatcagga cggcctggtt cacatctctt cattatcgaa caagtttgtg gaagatccgc
    68341 ataccgtggt gaaagcgggc gacattgtga aggtgaaagt gctggaagtg gatcttcagc
    68401 gtaaacgtat cgccctgact atgcgtctgg acgagcagcc tggcgaaacc aactctcgtc
    68461 gcggcggcgg taatgaacgc ccgcaaaaca accgcccggc agccaaacca cgcggtcgtg
    68521 aagcgcagcc tgccggtaat agcgcgatga tggatgcgct ggcggcagcg atgggtaaaa
    68581 aacgttaaac gcccggactg gcccctaaac ctgcgcgata aggcgttcac gccgcatctg
    68641 gcaacgaacg ctttgcctga cataaaagtg ccggagaata tctccggcac ttttattcca
    68701 cagccaaact cacaatatac tccgttaata tttatcactt cattaacaac tgaaacctta
    68761 attaaacatt agccagtccg ggtaactcac tattcgaatt atattttcgc tgcgatataa
    68821 ccttgagcca catcaacatt gagtcagatt attattcaaa ccaacattcg cacacatttt
    68881 aagtattgct gatagaaacc attctcatta tcattgtgtt gttgattatt taatctctcc
    68941 tttgttggca aatcatctgg tctcatgtcg cggtcaaacg ccccatgagg tagttatcca
    69001 gttaatgaga aacaagtagg cacctatgca atacactcca gatactgcgt ggaaaatcac
    69061 tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc taggcatgtt
    69121 acctggctcc tcttttgatg tggtgcgcgt cgctccactc ggcgacccca ttcatatcga
    69181 aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg gccttattag aagtggaagc
    69241 ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt aaccattggc ttaattggta
    69301 atccaaattc tggcaagaca accttattta accagctcac tggcgcacgt cagcgtgtag
    69361 gtaactgggc tggcgttacc gtcgaacgta aagaagggca attctccacc accgatcatc
    69421 aggtcacgct ggtggacctg cccggcacct attctctgac caccatctca tcgcagacct
    69481 cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac ctgctgatta
    69541 acgtggtgga tgcgtctaac cttgagcgta acctgtacct gacgctgcaa ctgctggaac
    69601 tcggcattcc ctgcatcgtg gcgctgaaca tgctcgacat tgccgagaag caaaatattc
    69661 gtattgaaat tgatgctcta tcggcacgtc ttggctgccc ggtgatcccg ctggtttcaa
    69721 cccgtggtcg cggtattgaa gcgctcaagc tggcgattga tcgctataaa gctaacgaga
    69781 atgtggaact ggtgcattac gcacagccgc tactcaacga agcagattca ctggcaaaag
    69841 tgatgccttc cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa atgctggaag
    69901 gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca gcatctggat gccaccctcg
    69961 cccgtctgcg taatgaaatg gatgatccgg cgctgcacat tgccgatgcg cgttaccagt
    70021 gcattgctgc catctgtgat gtggtaagca acaccctgac ggcagaaccc agccgtttca
    70081 ccactgcggt agataaaatc gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg
    70141 tgatgtacct gatgttcctg ctggctatca acatcggcgg ggcgttacag ccgctgttta
    70201 acgtcggctc cgtggcgcta tttgtgcatg gtattcaatg gattggctac acgctccact
    70261 tcccggactg gctgactatc ttcctcgccc agggcctggg tggcggtatt aacaccgtgc
    70321 tgccactggt gccgcagatc ggcatgatgt acctgttcct ctccttcctt gaggactccg
    70381 gctatatggc gcgtgcagcg tttgtgatgg accgcctgat gcaggcgctg ggcttgccgg
    70441 ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggcg
    70501 cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat catgatggca ccgtttatgt
    70561 cctgcggcgc gcgtctggct atcttcgcag tattcgcggc tgccttcttc gggcagaacg
    70621 gtgccctggc ggttttctcg ctgtatatgc tcggtatcgt gatggcggtg ctgactggcc
    70681 tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg
    70741 tctaccatgt accacacgtt aaaagcctga ttatccagac ctggcagcgt ctgaagggct
    70801 tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat tttcctgagc gctttcaaca
    70861 gcttctcgtt gagcgggaaa atcgtcgata acatcaacga ctcggcgctg gcttccgtca
    70921 gccgggtgat aacccctgtc ttcaagccga ttggcgtaca tgaagataac tggcaggcaa
    70981 cggttggcct gtttacgggc gcaatggcaa aagaagtggt ggtgggtacg ctcaacaccc
    71041 tctacaccgc agaaaatatt caggacgaag agttcaatcc ggcggaattc aacctcggtg
    71101 aagagctgtt cagtgcggta gatgaaacct ggcagagcct gaaagacacc ttcagcctta
    71161 gcgtactgat gaaccccatt gaagctagca aaggcaacgg tgaaatgggt accggggcga
    71221 tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc atacagctac ctgattttcg
    71281 tcctgctgta tgtaccatgt atctcggtga tgggagccat cgcccgtgaa tcaagccgtg
    71341 gctggatggg cttctccatc ctgtgggggc tgaatatcgc ttactcactg gcaacattgt
    71401 tctatcaggt cgccagctac agtcagcacc caacttacag cctggtgtgc attctggcgg
    71461 taatcctgtt taacatcgta gctatcggtc tgctgcgccg cgcgcgtagc cgggtggata
    71521 tcgaactgct ggcaacccgc aagtcggtaa gcagttgctg cgcagccagc accaccggtg
    71581 attgccatta atggcttcac ttattcaggt acgtgatttg ctggcgttac ggggccgtat
    71641 ggaagcgacc cagataagcc agacattgaa cactccgcag ccaatgatta acgccatgct
    71701 gcaacagctg gaaagcatgg gcaaagccgt gcggattcag gaagaacctg acggctgcct
    71761 ctctggcagt tgtaaaagct gcccggaagg caaagcctgt ctgcgcgagt ggtgggcgct
    71821 gcgttaacca tactccatcg cctgttttga aaagcccggt atgcgtttgc atccgggctt
    71881 ttttgcgtgc ggctttccat aaaaatgcaa ttcttgcagc gcaacgtaag ttcctctgaa
    71941 agcgtctcgc agggatgaaa actcgctaat gcacagatgt ggagtggcgc gtagagtcgc
    72001 ggcattcaaa caacaggtga aggaacgcca tgagtaaaaa gcagagttcc accccacatg
    72061 atgcgctgtt caaactcttt ttacgccaac cggagacggc acgcgatttt cttgcgtttc
    72121 atttaccggc acccattcac gcgctctgtg atatgaaaac cctcaagctg gagtcgagga
    72181 gctttattga tgacgatctg cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg
    72241 aacaaggacc aggatacatc tattgtctga ttgaacatca aagcacctca aacaaactga
    72301 tcgcatttcg catgatgcgt tacgctattg ccgcaatgca aaatcacctt gatgctggat
    72361 acaaaacgtt gccgatggtg gtgccattgt tgttttacca cggtattgaa agcccctatc
    72421 cctattcgct gtgttggctg gattgtttcg ccgatcccaa actggcaagg cagctttatg
    72481 cctccgcatt tccgctgatt gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc
    72541 gacgtatggc gctgctggag ttaattcaaa aacatattcg tcaacgcgat ctgatggggc
    72601 tggtagagca aatggcctgc ttattaagta gtggatacgc taatgacaga caaatcaaag
    72661 ggctgtttaa ttacatactg caaactggcg acgctgtacg ttttaacgat tttatcgacg
    72721 gcgttgccga acgttcaccg aaacacaagg agagtttaat gactattgcg gaaagattgc
    72781 ggcaggaggg ggaacaatcc aaagccctgc atatagccaa aataatgctt gaatccggag
    72841 tccctcttgc agacatcatg cgatttaccg ggctgtcaga agaagagttg gctgcggcga
    72901 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
    72961 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
    73021 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
    73081 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
    73141 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
    73201 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
    73261 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
    73321 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
    73381 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
    73441 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
    73501 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
    73561 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
    73621 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
    73681 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
    73741 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
    73801 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
    73861 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
    73921 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
    73981 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
    74041 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
    74101 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
    74161 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
    74221 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
    74281 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
    74341 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
    74401 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
    74461 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
    74521 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
    74581 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
    74641 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
    74701 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
    74761 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
    74821 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
    74881 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
    74941 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
    75001 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
    75061 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
    75121 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
    75181 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
    75241 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
    75301 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
    75361 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
    75421 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
    75481 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
    75541 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
    75601 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
    75661 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
    75721 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
    75781 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
    75841 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
    75901 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
    75961 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
    76021 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
    76081 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
    76141 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
    76201 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
    76261 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
    76321 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
    76381 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
    76441 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
    76501 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
    76561 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
    76621 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
    76681 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
    76741 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
    76801 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
    76861 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
    76921 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
    76981 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
    77041 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
    77101 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
    77161 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
    77221 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
    77281 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
    77341 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
    77401 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
    77461 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
    77521 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
    77581 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
    77641 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
    77701 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
    77761 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
    77821 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
    77881 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
    77941 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
    78001 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
    78061 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
    78121 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
    78181 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
    78241 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
    78301 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
    78361 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
    78421 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
    78481 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
    78541 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
    78601 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
    78661 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
    78721 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
    78781 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
    78841 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
    78901 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
    78961 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
    79021 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
    79081 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
    79141 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
    79201 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
    79261 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
    79321 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
    79381 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
    79441 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
    79501 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
    79561 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
    79621 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
    79681 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
    79741 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
    79801 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
    79861 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
    79921 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
    79981 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
    80041 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
    80101 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
    80161 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
    80221 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
    80281 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
    80341 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
    80401 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
    80461 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
    80521 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
    80581 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
    80641 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
    80701 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
    80761 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
    80821 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
    80881 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
    80941 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
    81001 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
    81061 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
    81121 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
    81181 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
    81241 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
    81301 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
    81361 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
    81421 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
    81481 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
    81541 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
    81601 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
    81661 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
    81721 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
    81781 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
    81841 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
    81901 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
    81961 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
    82021 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
    82081 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
    82141 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
    82201 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
    82261 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
    82321 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
    82381 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
    82441 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
    82501 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
    82561 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
    82621 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
    82681 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
    82741 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
    82801 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
    82861 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    82921 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    82981 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    83041 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    83101 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    83161 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    83221 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    83281 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    83341 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    83401 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    83461 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    83521 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    83581 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    83641 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    83701 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    83761 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    83821 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    83881 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    83941 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    84001 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    84061 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    84121 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    84181 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    84241 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    84301 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    84361 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    84421 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    84481 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    84541 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    84601 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    84661 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    84721 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    84781 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    84841 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    84901 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    84961 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    85021 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    85081 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    85141 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    85201 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    85261 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    85321 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    85381 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    85441 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    85501 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    85561 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    85621 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    85681 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    85741 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    85801 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    85861 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    85921 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    85981 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    86041 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    86101 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    86161 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    86221 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    86281 gcaggatctg cccgccgcct tcgccctgtg caccatccag cgcaatcatc cttttcatca
    86341 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    86401 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    86461 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    86521 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    86581 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    86641 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    86701 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    86761 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    86821 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    86881 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    86941 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    87001 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    87061 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    87121 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    87181 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    87241 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    87301 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    87361 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    87421 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    87481 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    87541 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    87601 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    87661 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    87721 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    87781 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    87841 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    87901 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    87961 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    88021 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    88081 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    88141 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    88201 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    88261 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    88321 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    88381 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    88441 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    88501 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    88561 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    88621 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    88681 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    88741 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    88801 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    88861 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    88921 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    88981 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    89041 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    89101 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    89161 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    89221 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    89281 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    89341 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    89401 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    89461 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    89521 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    89581 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    89641 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    89701 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    89761 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    89821 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    89881 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    89941 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    90001 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    90061 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    90121 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    90181 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    90241 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    90301 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    90361 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    90421 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    90481 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    90541 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    90601 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    90661 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    90721 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    90781 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    90841 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    90901 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    90961 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    91021 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    91081 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    91141 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    91201 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    91261 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    91321 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    91381 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    91441 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    91501 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    91561 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    91621 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    91681 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    91741 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    91801 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    91861 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    91921 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    91981 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    92041 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    92101 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    92161 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    92221 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    92281 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    92341 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    92401 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    92461 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    92521 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    92581 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    92641 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    92701 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    92761 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    92821 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    92881 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    92941 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    93001 cgtcgtaaat taacgtaagg tgatcaggtc agat
//
LOCUS       DAJTNW010000002        84552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNW010000002 DAJTNW010000000
VERSION     DAJTNW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO179_000090"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO179_000090"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5530315.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO179_000091"
     CDS             complement(1672..2730)
                     /locus_tag="OO179_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5530316.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO179_000092"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO179_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO179_000093"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO179_000093"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5530317.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO179_000094"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO179_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5530318.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO179_000095"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO179_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5530319.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO179_000096"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO179_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5530320.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO179_000097"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO179_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5530321.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO179_000098"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO179_000098"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5530322.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO179_000099"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO179_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5530323.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO179_000100"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO179_000100"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5530324.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO179_000101"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO179_000101"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5530325.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO179_000102"
     CDS             complement(17699..18322)
                     /locus_tag="OO179_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5530326.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO179_000103"
     CDS             18619..18894
                     /locus_tag="OO179_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530327.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO179_000104"
     CDS             complement(18971..19213)
                     /locus_tag="OO179_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5530328.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO179_000105"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO179_000105"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5530329.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO179_000106"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO179_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5530330.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO179_000107"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO179_000107"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5530331.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO179_000108"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO179_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO179_000109"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO179_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5530332.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO179_000110"
     CDS             24840..25697
                     /locus_tag="OO179_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5530333.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO179_000111"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO179_000111"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5530334.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28234..29250)
                     /gene="ypdE"
                     /locus_tag="OO179_000112"
     CDS             complement(28234..29250)
                     /gene="ypdE"
                     /locus_tag="OO179_000112"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5530335.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIAATFCRWSNFYLPLFNVLRVR
                     RLFN"
     gene            complement(29250..30333)
                     /gene="ypdF"
                     /locus_tag="OO179_000113"
                     /pseudo
     CDS             complement(29250..30333)
                     /gene="ypdF"
                     /locus_tag="OO179_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30348..31595)
                     /locus_tag="OO179_000114"
     CDS             complement(30348..31595)
                     /locus_tag="OO179_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5530336.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31617..31943)
                     /locus_tag="OO179_000115"
     CDS             complement(31617..31943)
                     /locus_tag="OO179_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5530337.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32162..33127)
                     /gene="glk"
                     /locus_tag="OO179_000116"
     CDS             complement(32162..33127)
                     /gene="glk"
                     /locus_tag="OO179_000116"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5530338.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33331..34587
                     /locus_tag="OO179_000117"
     CDS             33331..34587
                     /locus_tag="OO179_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5530339.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34702..35028
                     /locus_tag="OO179_000118"
     CDS             34702..35028
                     /locus_tag="OO179_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5530340.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35169..36407)
                     /locus_tag="OO179_000119"
     CDS             complement(35169..36407)
                     /locus_tag="OO179_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5530341.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36743..37945
                     /gene="nupC"
                     /locus_tag="OO179_000120"
     CDS             36743..37945
                     /gene="nupC"
                     /locus_tag="OO179_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5530342.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37995..40107)
                     /locus_tag="OO179_000121"
                     /pseudo
     CDS             complement(37995..40107)
                     /locus_tag="OO179_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40315..40390)
                     /locus_tag="OO179_000122"
     tRNA            complement(40315..40390)
                     /locus_tag="OO179_000122"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40355..40357),aa:Ala,seq:ggc)
     gene            complement(40430..40505)
                     /locus_tag="OO179_000123"
     tRNA            complement(40430..40505)
                     /locus_tag="OO179_000123"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40470..40472),aa:Ala,seq:ggc)
     gene            40726..41085
                     /locus_tag="OO179_000124"
     CDS             40726..41085
                     /locus_tag="OO179_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5530343.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41087..41479
                     /locus_tag="OO179_000125"
     CDS             41087..41479
                     /locus_tag="OO179_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5530344.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41531..42946)
                     /gene="gltX"
                     /locus_tag="OO179_000126"
     CDS             complement(41531..42946)
                     /gene="gltX"
                     /locus_tag="OO179_000126"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5530345.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43205..43280
                     /locus_tag="OO179_000127"
     tRNA            43205..43280
                     /locus_tag="OO179_000127"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43238..43240,aa:Val,seq:tac)
     gene            43325..43400
                     /locus_tag="OO179_000128"
     tRNA            43325..43400
                     /locus_tag="OO179_000128"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43358..43360,aa:Val,seq:tac)
     gene            43405..43480
                     /locus_tag="OO179_000129"
     tRNA            43405..43480
                     /locus_tag="OO179_000129"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43438..43440,aa:Lys,seq:ttt)
     gene            43511..43636
                     /locus_tag="OO179_000130"
     ncRNA           43511..43636
                     /ncRNA_class="other"
                     /locus_tag="OO179_000130"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43912..44253
                     /locus_tag="OO179_000131"
     CDS             43912..44253
                     /locus_tag="OO179_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5530346.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44244..45170)
                     /locus_tag="OO179_000132"
     CDS             complement(44244..45170)
                     /locus_tag="OO179_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5530347.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45260..46258
                     /locus_tag="OO179_000133"
     CDS             45260..46258
                     /locus_tag="OO179_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5530348.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46255..46473)
                     /locus_tag="OO179_000134"
     CDS             complement(46255..46473)
                     /locus_tag="OO179_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5530349.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46475..48490)
                     /gene="ligA"
                     /locus_tag="OO179_000135"
     CDS             complement(46475..48490)
                     /gene="ligA"
                     /locus_tag="OO179_000135"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5530350.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48561..49547)
                     /gene="zipA"
                     /locus_tag="OO179_000136"
     CDS             complement(48561..49547)
                     /gene="zipA"
                     /locus_tag="OO179_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5530351.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49777..50538
                     /gene="cysZ"
                     /locus_tag="OO179_000137"
     CDS             49777..50538
                     /gene="cysZ"
                     /locus_tag="OO179_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5530352.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50723..51694
                     /gene="cysK"
                     /locus_tag="OO179_000138"
     CDS             50723..51694
                     /gene="cysK"
                     /locus_tag="OO179_000138"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5530353.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52078..52335
                     /gene="ptsH"
                     /locus_tag="OO179_000139"
     CDS             52078..52335
                     /gene="ptsH"
                     /locus_tag="OO179_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5530354.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52380..54107
                     /gene="ptsI"
                     /locus_tag="OO179_000140"
     CDS             52380..54107
                     /gene="ptsI"
                     /locus_tag="OO179_000140"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5530355.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54148..54657
                     /gene="crr"
                     /locus_tag="OO179_000141"
     CDS             54148..54657
                     /gene="crr"
                     /locus_tag="OO179_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5530356.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54699..55550)
                     /gene="pdxK"
                     /locus_tag="OO179_000142"
                     /gene_synonym="thiJ"
     CDS             complement(54699..55550)
                     /gene="pdxK"
                     /locus_tag="OO179_000142"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5530357.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQMVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55655..56029
                     /locus_tag="OO179_000143"
     CDS             55655..56029
                     /locus_tag="OO179_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5530358.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56062..56785
                     /locus_tag="OO179_000144"
                     /pseudo
     CDS             56062..56785
                     /locus_tag="OO179_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56989..57900)
                     /gene="cysM"
                     /locus_tag="OO179_000145"
     CDS             complement(56989..57900)
                     /gene="cysM"
                     /locus_tag="OO179_000145"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5530359.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58034..59131)
                     /gene="cysA"
                     /locus_tag="OO179_000146"
     CDS             complement(58034..59131)
                     /gene="cysA"
                     /locus_tag="OO179_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5530360.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59121..59996)
                     /gene="cysW"
                     /locus_tag="OO179_000147"
     CDS             complement(59121..59996)
                     /gene="cysW"
                     /locus_tag="OO179_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5530361.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59996..60829)
                     /gene="cysT"
                     /locus_tag="OO179_000148"
     CDS             complement(59996..60829)
                     /gene="cysT"
                     /locus_tag="OO179_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5530362.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60829..61845)
                     /gene="cysP"
                     /locus_tag="OO179_000149"
     CDS             complement(60829..61845)
                     /gene="cysP"
                     /locus_tag="OO179_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5530363.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62149..62940)
                     /gene="ucpA"
                     /locus_tag="OO179_000150"
     CDS             complement(62149..62940)
                     /gene="ucpA"
                     /locus_tag="OO179_000150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5530364.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63127..63927)
                     /gene="murR"
                     /locus_tag="OO179_000151"
     CDS             complement(63127..63927)
                     /gene="murR"
                     /locus_tag="OO179_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5530365.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64091..64987
                     /gene="murQ"
                     /locus_tag="OO179_000152"
     CDS             64091..64987
                     /gene="murQ"
                     /locus_tag="OO179_000152"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5530366.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64991..66415
                     /gene="murP"
                     /locus_tag="OO179_000153"
     CDS             64991..66415
                     /gene="murP"
                     /locus_tag="OO179_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5530367.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66595..67494)
                     /gene="yfeX"
                     /locus_tag="OO179_000154"
     CDS             complement(66595..67494)
                     /gene="yfeX"
                     /locus_tag="OO179_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5530368.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67590..68165)
                     /locus_tag="OO179_000155"
     CDS             complement(67590..68165)
                     /locus_tag="OO179_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5530369.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68226..68675)
                     /locus_tag="OO179_000156"
     CDS             complement(68226..68675)
                     /locus_tag="OO179_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5530370.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68662..69087)
                     /locus_tag="OO179_000157"
     CDS             complement(68662..69087)
                     /locus_tag="OO179_000157"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5530371.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69301..70170
                     /gene="amiA"
                     /locus_tag="OO179_000158"
     CDS             69301..70170
                     /gene="amiA"
                     /locus_tag="OO179_000158"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5530372.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70174..71073
                     /gene="hemF"
                     /locus_tag="OO179_000159"
     CDS             70174..71073
                     /gene="hemF"
                     /locus_tag="OO179_000159"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5530373.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71079..72131)
                     /gene="eutR"
                     /locus_tag="OO179_000160"
                     /pseudo
     CDS             complement(71079..72131)
                     /gene="eutR"
                     /locus_tag="OO179_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72177..72677)
                     /gene="eutK"
                     /locus_tag="OO179_000161"
     CDS             complement(72177..72677)
                     /gene="eutK"
                     /locus_tag="OO179_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5530374.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72690..73349)
                     /gene="eutL"
                     /locus_tag="OO179_000162"
     CDS             complement(72690..73349)
                     /gene="eutL"
                     /locus_tag="OO179_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5530375.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74531..75322
                     /locus_tag="OO179_000163"
     CDS             74531..75322
                     /locus_tag="OO179_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530376.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            76042..76437
                     /locus_tag="OO179_000164"
     CDS             76042..76437
                     /locus_tag="OO179_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5530377.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76455..76718
                     /locus_tag="OO179_000165"
     CDS             76455..76718
                     /locus_tag="OO179_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530378.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76741..77283
                     /locus_tag="OO179_000166"
     CDS             76741..77283
                     /locus_tag="OO179_000166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530379.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77422..77709
                     /locus_tag="OO179_000167"
     CDS             77422..77709
                     /locus_tag="OO179_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530380.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77748..77954
                     /locus_tag="OO179_000168"
     CDS             77748..77954
                     /locus_tag="OO179_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530381.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78266..78751
                     /locus_tag="OO179_000169"
     CDS             78266..78751
                     /locus_tag="OO179_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5530382.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78732..79010
                     /locus_tag="OO179_000170"
     CDS             78732..79010
                     /locus_tag="OO179_000170"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530383.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            79003..80463
                     /locus_tag="OO179_000171"
     CDS             79003..80463
                     /locus_tag="OO179_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5530384.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80497..81306
                     /gene="murI"
                     /locus_tag="OO179_000172"
     CDS             80497..81306
                     /gene="murI"
                     /locus_tag="OO179_000172"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5530385.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81425..82119)
                     /locus_tag="OO179_000173"
     CDS             complement(join(81425..81873,81873..82119))
                     /locus_tag="OO179_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5530386.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82638..84011)
                     /gene="eutA"
                     /locus_tag="OO179_000174"
     CDS             complement(82638..84011)
                     /gene="eutA"
                     /locus_tag="OO179_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5530387.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83986..84465)
                     /gene="eutP"
                     /locus_tag="OO179_000175"
     CDS             complement(83986..84465)
                     /gene="eutP"
                     /locus_tag="OO179_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5530388.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84490..>84552)
                     /locus_tag="OO179_000176"
     CDS             complement(84490..>84552)
                     /locus_tag="OO179_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530389.1"
                     /translation="KSVELHDKVIGHYLNIKHYQ"
BASE COUNT        21959 a        21405 c        20059 g        21129 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcgatgc
    28321 cgcgcaatgt ccgtggcggg tggcaggccc tattaccaat gtgggtacgc cagtaccggt
    28381 taagtgcacc gctccaccgt ccgtgccgcc gttactgaac atatccgcct gcaacggaac
    28441 gccaatttct gccgccacgg tttcaatcca cgcggtaagt tttggcggcg caatcagcga
    28501 tttatcgctt aacaccagca tcggcccgtt accaatctga cgatggttag ccgcgccata
    28561 atcaaagttt ttcgcccagc aggcggtatc aagcacaatg gcgacgtccg gcgacaccgc
    28621 gcgggtagca gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg ccaccagcca
    28681 cacttccgca ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca gcagatagca
    28741 actgaggcga tcatcaaagg ctttgcccat cacccgctgg tgagggagga cctgaaaagt
    28801 ggtatcgaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt cgtcataggt
    28861 gcgcgcgcca atgtccacgc gcatggcgct aacgtcattc ccctgccggt cgccgtcaag
    28921 caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca gctggcgggc
    28981 agccatgcgc acgttgccaa ccggcagcac gtcaatagcg ccttcgcggg agatgctgcg
    29041 caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggac ctgtcgattc
    29101 attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt gcaggcgagc
    29161 cgcttcttcc agcaggatct gccgcacttc ctgttccgag gaggcgatcg catctgcctc
    29221 gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa cactgttttc
    29281 ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc ttcgatgcgc
    29341 acgccccctt gccctagcaa ataaatcccc ggctccacgg tcagcaacat gcctggctgt
    29401 agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat gccgatagcg
    29461 tgaccagtgt tatgaccgaa atagtcgcca aatccagcct cggtaatgac ccggcgcgcg
    29521 gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg cagagattgc cgcgagctgt
    29581 gcctgcagga caatctgata gacgtcaaac agcgggtgag attcggcgct caccccttcg
    29641 ccattcacca gcaaggtgcg cgtcatatca gcagtagccc tgatacagcg caccaaaatc
    29701 gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg gctttgccgt gcggcagcgc
    29761 cccgcgccag ccgctggcga caatggtgtc aaaagaggct ttttctgcgc cctgctggcg
    29821 cataaaccac tccagttcag cggctatctc gcgctcgctc atccccgcct gaataaagcg
    29881 gcgaatatgc tccgctccgc gatcggcaat cccacaggcg aggcggatta tctccacctc
    29941 ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctaaccagtt tcgcattgag
    30001 ttcagacttc cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt
    30061 ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg gtaagcgtgt tcgtcgcgtc
    30121 aagcaaatgc agctggtagc cttgcgcacg ggcttccacc tcaacgaaat agcgcgaatc
    30181 taccagaatg tgcgcacttt cacggctaat aaccacatag cctgatccgg tggagatccc
    30241 cagatgcggc tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcacctt
    30301 aagccagttg cgcagcgaag cgagtaatgt catctggatt ttccttctta caggctatcg
    30361 attaacaatt tgcctttgcg gaacatcatc agacgcagga acaccaccat cagcgcagta
    30421 atgattgccc ccagcgcgat ccccgccata taaacgccca ggttagtgac cagcggccat
    30481 gcccagatag cggattccgg gaaccattgc actgcgccca gccagacagc tgcggttgag
    30541 ccgacaatcg cgccgaccat atatgacgga atagcggtga tggggctttc cagcgcaaac
    30601 ggaatcgcac cctcactaat ccccataaag gccaggaaca tcgcggtctt accctgcgga
    30661 taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcaataat ggtcgccaga
    30721 cccagaccaa tcggcggaat aacgatagcg atagaacgag cggtgaccgg taatacgtgg
    30781 tcagtggtaa agctgaaggc aacgaaacct gcggctttgt tgatcgggcc accaaggtcg
    30841 attgctgtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcggca
    30901 gtcagcacag tacggatacc gccgttgatc cagccaccaa agggagtgat gacgtagtac
    30961 atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct
    31021 aacaggaaat ccggtaactg gattttctgg ttcatccatt tcacaaggta accggcaacg
    31081 atagaaataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc
    31141 atagtgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa cccgccgata
    31201 aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaatgc agcaaacatc
    31261 ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg acagccaggc aaatttaagt
    31321 aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatctca
    31381 gcaggaattt taagccagct ataagcaatc agctggctga aagcgaggat cacaccgccc
    31441 ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc
    31501 cagaagctgc ttttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca
    31561 ggcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac
    31621 tgttgttcag aggcaatcat ctcttcgatt tctttgatga tgcccgcagc gtttttaatt
    31681 gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgttcgtt atcttccggg
    31741 gtaactgcca cggaatggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga
    31801 ttctggatgc cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc
    31861 gcttcttcca gcacctgagc ggccataaaa gtgtgagcta ggcccattgg gcaggcacat
    31921 aaggcaatca gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa
    31981 ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta
    32041 atcccgctgt acggaagtgt gacgcaggtc ggccttgtga ggggacacaa agaaaaattg
    32101 aattaactgt atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga
    32161 tttacagaat gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat
    32221 agtcatggac gatgagatac accggaatat catggacata ttctttaaag cgccctttat
    32281 cttcaaatgc ggcacggaaa ccggaggctt tgaagaactc aaggaagcgc ggcacgatac
    32341 cgcccgcaat aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac
    32401 ggcccataat gacgcaaaac agcgacaatg cgcggcggca atcggtgcag ctgtcagcca
    32461 gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg tcagctttca
    32521 caattgcgcg atacaaattc accagcccag ggccagaaag cacgcgctcc gccgaaacat
    32581 gaccaatttc cgcacgcaat atttcgagga taatggcctc ttcttcacta ttcggcgcaa
    32641 aatcaacgtg accgccttcg cccggcaagc ttacccaacg cttatcgaca tggaccagat
    32701 gcgcaacccc aagccccgtt ccggcaccgt aaaccgcaat aggcttacct tcgaccggtt
    32761 ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag
    32821 cggtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa
    32881 ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga
    32941 tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg
    33001 cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg
    33061 caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact
    33121 ttgtcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc
    33181 tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca
    33241 caattacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact
    33301 aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg
    33361 ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct gattgtggtg
    33421 atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg
    33481 atagcccagg attcacccct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg
    33541 gggttggtta tccgtttcag ccagggtcat gccggaccag accccgcctg tgaaccgctg
    33601 atcggcgcac cggttccgcc ctctgcgcta cctggactta tcgtagcatt aattctcggt
    33661 cttgctggcg gcgtcagcct ggggccggaa catccgatca tgaccgtcaa tatcgccctt
    33721 gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta
    33781 gcctctgccg gaaccatcgg cgcgctgttt ggtacacctg ttgcggcggc gttgatattt
    33841 tcgcaaacct taaatggcag tagtgaagtt ccgctatggg atcgtctttt tgcgccgtta
    33901 atggcggcag cagctggtgc acttactacc ggattatttt tccatcccca tttttcactg
    33961 cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg
    34021 gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgcgatg
    34081 atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat tctcggaatt
    34141 ctgggggtta ttggtggacc agtttcgctg tttaaagggc tggatgagat gcagcagatg
    34201 gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtaat taaacttgcc
    34261 gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt
    34321 gtcggcgtgg cattagggtt gatgctgcat gagcacgttc ccgccgttcc agcggcaata
    34381 accgtttcct gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt
    34441 ctttttatgg cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc
    34501 atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag
    34561 caacagccac cccacgataa tgtttagcaa aaaatgctct cctttatgat ttaagagtta
    34621 tggcgatgat acggggcttt acctccccgt aatattgcgt taacaggccg ctgacggctt
    34681 cgatcgtgaa ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca
    34741 tttaccccgc ttgcagcaaa tgcaggtgaa atcaccctac tgccatcgat caaattacaa
    34801 attggcgatc gcgatcatta cggtaattac tgggacggtg gtcactggcg agatcgtgat
    34861 tactggcatc gcaattatga atggcgcaaa aaccgctggt ggcgtcatga taatggctat
    34921 catcgtggct gggataagcg taaagcatat gagcgtggct atcgtgaagg ctggcgcgat
    34981 cgtgacgatc atcgcggaaa aggccgcgga catgggcacc gccattaaac gcgtcctcca
    35041 atggagcaca atgcctgatg cgacgctagc gcgtcttatc aggcctacaa accggctctt
    35101 taacgcgtag ggcggataag gcgtttacgc cgcatccgcc aatagtgcca gatgcgacgc
    35161 tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag cgccacgacc
    35221 agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag atcgcccatc
    35281 aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat accaaaactt
    35341 aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag aatgacaata
    35401 aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa gcgaatgaat
    35461 ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag tccggcagca
    35521 accagactta acccaaagac cgttgccgca gcatggctta acagcggttg cagcgtcaga
    35581 taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc cgccgcagct
    35641 gtagccatca tcaccagatt gacaaaaccg gcaatcgtca tggcgatagc cacatcccat
    35701 ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt gagcgaagag
    35761 tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc caggaagacc
    35821 gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc caggttaggc
    35881 tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa cccgccaatc
    35941 actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa agtcgcgatc
    36001 cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa accgatcgcc
    36061 gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg aacccaatag
    36121 aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt tttaccggtg
    36181 gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt ggcccaaacg
    36241 acaacccaca gtagctgata gccgaagcta gcacccgcct gaatattggt cgcaaagtta
    36301 ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc
    36361 ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt gtgcctctaa
    36421 aacatagcct ttgctatgtt tcatgctatg ctaaacgaga atgattatca aattcattta
    36481 aatggattgt ggtgatttct ctgatagacc aagattatga ttacgaaaag attgatgact
    36541 tgaatgttat ggttatgttt aatgttagca catttacata actttcagct tccatacaca
    36601 acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga cgtcattata
    36661 gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac
    36721 ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt
    36781 tgttgtgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt
    36841 tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg
    36901 tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat ttgccaacga
    36961 agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa
    37021 agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc acattcgcgt
    37081 gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg gtatgggcaa
    37141 actggaatcc tttaacgccg tcagctccct gattctgggt cagtcagaaa actttattgc
    37201 ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg ctgccacggc
    37261 aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg aaccgaaata
    37321 cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa
    37381 tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc acgaaggtca
    37441 gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg ccattatcgt
    37501 tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac tgtttgccac
    37561 cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct acatcttcta
    37621 tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat
    37681 ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc
    37741 cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctagtttcct tcgctaactt
    37801 ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc aaggtaacgt
    37861 ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg tgctgtctgc
    37921 gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat cattccccgg
    37981 cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata cccttgcaga
    38041 tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac aaactccgcg
    38101 accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat catcgcatcc
    38161 agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat atcagcctgc
    38221 aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat cgcaatccgg
    38281 aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt agaaaacgcc
    38341 tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg cttaaacaga
    38401 cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa attgactgaa
    38461 aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa ggattccagc
    38521 acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag aaacttatcc
    38581 ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata accttcaccc
    38641 tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa cgccgtgcga
    38701 atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc ctctctgcta
    38761 tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct taactgcccc
    38821 aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc accatagccc
    38881 atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt aaccatatgc
    38941 tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact acccggcaat
    39001 tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat tgagcggata
    39061 cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt atcaatgcgc
    39121 aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc acgaaagttg
    39181 ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc agcggcgatt aagccattca
    39241 ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat cagcaccgcg
    39301 agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg gttgtaattc
    39361 agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg aagattaccg
    39421 accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat aaagtcgttt
    39481 tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca gcttaaggta
    39541 aatgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39601 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39661 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    39721 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    39781 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    39841 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    39901 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    39961 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    40021 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    40081 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    40141 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    40201 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtagact gtattacaaa
    40261 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    40321 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    40381 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    40441 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    40501 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40561 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40621 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40681 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtt caaggagagg
    40741 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    40801 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg caaagcccga
    40861 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    40921 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    40981 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    41041 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    41101 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    41161 ccgttggacc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    41221 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    41281 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    41341 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    41401 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    41461 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    41521 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41581 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41641 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    41701 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    41761 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    41821 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    41881 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    41941 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    42001 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    42061 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    42121 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    42181 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    42241 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    42301 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    42361 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    42421 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    42481 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42541 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42601 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42661 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    42721 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    42781 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    42841 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    42901 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    42961 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    43021 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    43081 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    43141 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    43201 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43261 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    43321 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43381 gataccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
    43441 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
    43501 ggcgcttttt tgctatctgc gatactcaaa gattcgaacc tgcagcaggt ttgagttgag
    43561 cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat
    43621 cctgcacgac ccaccaatgc aaaaaagcgc cctaaaggcg cttttttgct atctgcgata
    43681 ctcaaagatt cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc
    43741 gcggcgacgg cccgaaaggc gagcgaagcg agtcatcctg cacgacccac caatgcaaaa
    43801 aagcgcccta aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa
    43861 gttccttgtt cttctaccga tattacctcg tacagtgacg aggactgaac aatggccact
    43921 atctcttcaa cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt
    43981 agcgatgttg cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacaa
    44041 attaaagaga ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg
    44101 attcaacagc aaatcaccct actggaaaca caactggcgc agttgcagaa acagcaggca
    44161 gaaaaggcgc aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt
    44221 gaaaaaacga ctcatatcga tatctatatc tgatacaacg gatccccttc ccgaccagcc
    44281 attagcgcat tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt
    44341 gaacggttct tcctgcgcac cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta
    44401 accaccagtg ggctaccttc aggcaatggt agcgccagtg caggaagaat actgatgccg
    44461 atgcctgcgg ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc
    44521 gcctgaatac cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa
    44581 tcctgcaaca ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccacggcc
    44641 aaagcgctat cgcgatggca aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc
    44701 agatcgccca ctgggcctgg atcgatgacg atgccaaagt cgacatctcc ctggcgaata
    44761 ctttccatca cccactgctg cggacgatca tgcaggacaa actgaatatc tggatagcgg
    44821 cgatggcttt cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg
    44881 acccgaactt ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta
    44941 agttcatcca gcagtcgctc aagacgcaaa gccaactgct gccctgcatc tgtaagcacc
    45001 acttcacgcg tggttctgtc cagcaggcga acaccagtat gattttccag ctccttcaca
    45061 ctgtgactta ctgccgactg gctcaagccg atacgctctc ctgcacgact aaaacttttc
    45121 tcctgcgcta ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt
    45181 catgaatgga tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca
    45241 tcgatcatct ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct
    45301 gatcacggtg gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt
    45361 tgaaaatctg accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc
    45421 gcgtgaggcg attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag
    45481 caccttcgtg ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt
    45541 cgacccgatg ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc
    45601 tgcaatcgcc ttcacgtcaa tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc
    45661 agcatccagc ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaatgt
    45721 tcacggtgca aggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc
    45781 gtttgtgttg gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa
    45841 atggattgcg aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga
    45901 agccgtcgtt aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt
    45961 ggtcagctgc gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact
    46021 gagcttcaat aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc
    46081 aaatggcatc ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct
    46141 gcccctgatg attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata
    46201 caaacgccag accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag
    46261 cggacgcttc aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat
    46321 ttgcattagc tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag
    46381 atactcctcc ggcaggtcaa ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt
    46441 attggctatt tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt
    46501 ccgcttcgtc gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag
    46561 cttcacccgc tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca
    46621 gttcgaccag tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca
    46681 ccacggtttt accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag
    46741 gccagtgaac accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa
    46801 agaagttgtg aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt
    46861 caatcgaagc ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg
    46921 tggcctcgcc gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt
    46981 tcgctttttc cagcgcgtta accacgtttt gtgccaattt tggccccata cgctccagtc
    47041 cggtcagttt gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt
    47101 caaccagctg atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa
    47161 caaagtgttt cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg
    47221 caaccgcttc accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga
    47281 atacaacctc acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga
    47341 tcacgtcgcc agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga
    47401 tttcatccgc attgtgtaag gttgcgttac tcaccagcac gccagcaaca tggacaggtt
    47461 ccagacgcgc aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca
    47521 caaaggtcat ctgctcctgc gccgggaatt taaacgcaac cgcccagcgc ggggcacgcg
    47581 cgacaaagcc aagctgctcc tgctgttcca gtgagttgac cttaatcacc acgccgtcga
    47641 tatcaaagcc cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt
    47701 ccgccgattc acaaagcgtt acccgatcgc tgaccggcac cccccacttt ttaaattgca
    47761 gtaaacggcc aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc
    47821 agaaaaaagt gagcggtcgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag
    47881 ctgccgcatt acgtgggtta gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa
    47941 tcttttcgaa ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga
    48001 tattctctcc gtgcagcttc agcggaatgg cgcgaatagt acgcacatta gacgtgatat
    48061 cttccccggt ggtgccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca
    48121 gaatactgac ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgt
    48181 ttttcagacg gtcctgcaca cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt
    48241 tatccagtga cagcattggt acttcatggc gtatctggct gaaagctgcc agcggcgcag
    48301 cacctacacg ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct
    48361 cgcgcagttc gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca
    48421 catgataaag atattcatga tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga
    48481 ttgattccat atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga
    48541 gttaatttgc cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg
    48601 tactcgcgca atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc
    48661 acttcatcgg caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca
    48721 ccgtaagacg gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca
    48781 ggatcaaagg ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca
    48841 tccgggctaa gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga
    48901 atgctgttaa gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg
    48961 ataatcaccg cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc
    49021 tcaggctgtg gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt
    49081 gctgaatgca caggctgcgg cgctggcgcg acctgtggcg aaactggctg ctgcaacggc
    49141 tgttcaggct gcggctgata ggcaggctgc tgcaccggct gcgctggacg cggagcatgt
    49201 tgcggcggta cctgcgcttc aggcggctgc tggaccggtt gacgcggctg cgcagacgca
    49261 taaggcggta ggtactggtg ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta
    49321 gccggggcat gattcacgcg gtgaacacga acctcaccaa cgccctcatc atcttcgaca
    49381 tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg
    49441 aacatagaag atcgttcttt acggctggtc cagaaaccat gtaccagtaa agcgattatg
    49501 gcgatcgcgc caacaatgat taatatcaga cgcaaatcct gcatcattat attctctgtt
    49561 gttctaacac cttgccacca cggcaaacat ttactcacta agagtatttg ccgattacct
    49621 caagtgcaag tgcactatta actttcacag cacaaagata gatgaaatcg tgctttttgc
    49681 tgttttttcg aacatatcct aactgtccat tgcgcaatta cccggtaaaa tacgcagaat
    49741 tttcctggga ttggtcaaaa ggagctcatc ctgattatgg tttcatcatt cacatctgcc
    49801 ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt
    49861 cggcgtttcg ttattttacc gctgctggtc aatattttgt tgatgggggg cgcattctgg
    49921 tggctcttta cacagctcga tgtctggatc ccgactttca tgagttacgt tccggactgg
    49981 ctacaatggc tgagttatct gttgtggcct ctggctgtca tctctgtgct gttagtgttt
    50041 ggctatttct tctccacgat tgctaactgg attgccgctc cgtttaacgg tttattggct
    50101 gaacaactgg aagcacgatt gactggcgct acaccgccag ataccgggat tttcggtatc
    50161 atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa aatttgtctg gtatctgccg
    50221 cgcgcaattg tattactaat tctttacttc atccctggta ttgggcaaac cgtcgcgccg
    50281 gtactgtggt tcctgtttag cgcctggatg ttagccatcc agtattgcga ttaccccttc
    50341 gataaccaca aagtgccgtt taaagagatg cgcaccgccc tgcgcacacg caaaatcacc
    50401 aatatgcagt ttggtgcttt aaccagcctg tttacgatga tcccgctgct taatctgttc
    50461 atcatgcccg ttgccgtttg tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa
    50521 cacgcgatgt ggcggtaaca atctaccggt tattttgtaa accgtttgtg tgaaacaggg
    50581 gtggcttatg ccgcccctta ttccatcttg catgtcatta tttcccttct gtatatagat
    50641 atgctaaatc cttacttccg catattctct gagcgggtat gctacctgtt gtatcccaat
    50701 ttcatacagt taaggacagg ccatgagtaa gatttttgaa gataactcgc tgactatcgg
    50761 tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga
    50821 atctcgtaac cccagcttca gcgttaagtg ccgtatcggt gccaacatga tttgggatgc
    50881 cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt gaaccgacca gcggtaatac
    50941 cgggattgca ctggcctatg tagctgccgc tcgcggttac aaactcaccc tgaccatgcc
    51001 agaaaccatg agtattgaac gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct
    51061 gacggaaggt gctaaaggca tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag
    51121 caatccagag aaatacctgc tgctgcaaca attcagcaat ccggcaaacc ctgaaattca
    51181 cgaaaaaacc accggtccgg aaatctggga agataccgac ggtcaggttg atgtatttat
    51241 tgctggcgtt gggactggcg gtacgctgac tggcgtcagc cgctacatta aaggcaccaa
    51301 aggcaagacc gatcttatct ctgtcgccgt tgagccaacc gattctccag ttatcgccca
    51361 ggtgctggca ggtgaagaga ttaaacctgg cccgcataaa attcagggta ttggcgctgg
    51421 ttttatcccg gctaacctcg atctcaagct ggtcgataaa gtcattggca tcaccaatga
    51481 agaagccatt tctaccgccc gtcgtctaat ggaagaagaa ggtattcttg caggtatctc
    51541 ttctggagca gctgttgccg cagcgttgaa actacaagaa gatgaaagct ttaccaacaa
    51601 gaatattgtg gttattctac catcatcggg tgagcgttat ttaagcaccg cattgtttgc
    51661 cgatctcttc actgagaaag aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa
    51721 aagcaccttt ttaggtgctt ttttgtggcc tgcttcaaac tttcgcccct cctggcattg
    51781 attcagcctg tcggaactgg tatttaacca gactaattat tttgatgcgc gaaattaatc
    51841 gttacaggaa aagccaaagc tgaatcgatt ttatgatttg gttcaattct tcctttagcg
    51901 gcataatgct taatgacgta cgaaacgtca gcggtcaaca cccgccagca atggactgta
    51961 ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct aacaatacag gctaaagtcg
    52021 aaccgccagg ctagacttta gttccacaac actaaaccta taagttgggg aaatacaatg
    52081 ttccagcaag aagttaccat taccgctccg aacggtctgc acacccgccc tgctgcccag
    52141 tttgtaaaag aagctaaggg cttcacttct gaaattactg tgacttccaa cggcaaaagc
    52201 gccagcgcga aaagcctgtt taaactgcag actctgggcc tgactcaagg taccgttgtg
    52261 actatctccg cagaaggcga agacgagcag aaagcggttg aacatctggt taaactgatg
    52321 gcggaactcg agtaatttcc cgggttcttt taaaaatcag tcacaagtaa ggtagggtta
    52381 tgatttcagg cattttagca tccccgggta tcgctttcgg taaagctctg cttctgaaag
    52441 aagacgaaat tgtcattgac cggaaaaaaa tttctgccga ccaggttgat caggaagttg
    52501 aacgttttct gagcggtcgt gccaaggcat cagcccagct ggaaacgatc aaaacgaaag
    52561 ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga agggcatatt atgctgctcg
    52621 aagatgagga gctggagcag gaaatcatag ccctgattaa agataaacac atgacagctg
    52681 acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc cctggaagag ctggatgatg
    52741 aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca
    52801 tcctgggcct gaagattatc gacctgagcg ccattcagga tgaagtcatt ctggttgccg
    52861 ctgacctgac gccgtccgaa accgcacagc tgaacctgaa gaaggtgctg ggtttcatca
    52921 ccgacgcggg tggccgtact tcccacacct ctatcatggc gcgttctctg gaactgcctg
    52981 ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa tgacgactat ctgattctgg
    53041 atgccgtaaa taatcaggtt tacgtcaatc caaccaacga agttattgat aaaatgcgcg
    53101 ctgttcagga gcaagtggct tctgaaaaag cagagcttgc taaactgaaa gatctgccag
    53161 ctattacgct ggacggtcac caggtagaag tatgcgctaa cattggtacg gttcgtgacg
    53221 ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct gtatcgtact gagttcctgt
    53281 tcatggaccg cgacgcgctg cccactgaag aagaacagtt tgctgcttac aaagcagtgg
    53341 ctgaagcgtg tggctcgcag gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag
    53401 agctgccata catgaacttc ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc
    53461 gtatcgcgat ggatcgtaaa gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct
    53521 cggctttcgg taaattgcgc attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg
    53581 cactgcgcaa agagatcgaa atctacaaac aggaactgcg cgacgaaggt aaagcgtttg
    53641 acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc
    53701 atttagccaa agaagttgat ttctttagta tcggcaccaa tgatttaacg cagtacactc
    53761 tggcagttga ccgtggtaat gatatgattt cacaccttta ccagccaatg tcaccgtccg
    53821 tgctgaactt gatcaagcaa gttattgatg cttctcatgc tgaaggcaaa tggactggca
    53881 tgtgtggtga gcttgctggc gatgaacgtg ctacacttct gttgctgggg atgggtctgg
    53941 acgaattctc tatgagcgcc atttctatcc cgcgcattaa gaagattatc cgtaacacga
    54001 acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc tcaaccgaca acggacgagt
    54061 taatgacgct ggttaacaag ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg
    54121 gcccaattta ctgcttagga gaagatcatg ggtttgttcg ataaactgaa atctctggtt
    54181 tccgacgaca aaaaggatac cggaactatt gagatcattg ctccgctctc tggcgagatc
    54241 gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt
    54301 gctatcaaac caacgggtaa caaaatggtc gcgcctgtag acggtaccat tggtaaaatc
    54361 tttgaaacca atcacgcgtt ctctatcgaa tctgatagcg gcgttgaact gttcgtccac
    54421 ttcggtatcg acaccgttga actgaaaggc gaaggcttca agcgtattgc tgaagaaggt
    54481 cagcgcgtga aagttggcga tactgtcatt gaatttgatc tgccgctgct ggaagagaaa
    54541 gccaagtcta ccctgactcc ggttgttatc tccaacatgg acgaaatcaa agaactgatc
    54601 aaactgtccg gtagcgtaac cgtgggtgaa accccggtta tccgcatcaa gaagtaattc
    54661 ttgccgcagt gaaaaatggc gcccatcggc gccatttttt atgcttccgc cagcggcggc
    54721 aaaatcaatt catcgctctc atgctgctgg gtgtagcgca tcacttccag tacgcgcaac
    54781 cccgcacggt gcaccgcatc gtttaacgcc ttccctttca gcaaaccact gataagctga
    54841 gcacaaaaca ggtcgccagt ccctttcagg tcggttttta cccgtgaatg ggaaatgaca
    54901 ttcacgctgt cggcactgac caccaccatc tgcatctcct gattttcttc attaccggag
    54961 gcgctggtaa tcaccaccca ttttaatgtg tctgaaagca gactttttgc ggcagcaatg
    55021 gcactgtcga gatcgcggca atttttaccg gtcaggattt ccagctcaaa gatattgggg
    55081 gtaattccct gcgccagcgg cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg
    55141 acataaattc cgctatcaat atcgccaatc accggatcga ccatgatcaa taggtcagga
    55201 tggtctttgc gtagcgcagt cagccactcg gcaaggattt tgatttgcga tgccgttccc
    55261 atatagcccg tggttacagc acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc
    55321 aaatagccgc taaaccattc gtccggaatc gcaccaccgt agaaagtgtc ataatgcggc
    55381 gtattgctca gcaataccgt cggcacggca aagacattca agccgttctg ttttatagca
    55441 ggcaccgcaa tgctgttgcc tacgcttccg tacactacct gcgactgcac ggcgacgata
    55501 tccgcctgca gcgccctact cttatcgtta aacaacaaca aactactcat ttaatttttt
    55561 ctccttgccg atgatcctca tcgtattcca accgaaactt tacctgattc tggcagtcaa
    55621 atcggctatt acaaaacaaa gataaggtaa ttcagtgaag aaaatcattt gtctggccat
    55681 tacgctgcta atgacactcc ccgcttacgc gaagttaact gttcatgaag aagcccgcat
    55741 caacgccatg ctggagggat tagcacagaa aaaggatttg atatttgtgc gcaacggtga
    55801 tgaacatacc tgcgatgaag cggtttccca cctgcgtctg aagctcggca atacccgtaa
    55861 ccgcattgac actgccgagc agtttattga taaggtagct tcgtcgtcat cgattaccgg
    55921 gaagccgtat atcgtgaaga tccccggtaa gagcgatgag aacgcacagc cttttttaca
    55981 tgcgttaatt gcgcagacgg ataaaacggt gcctgcggaa ggaaattaat ccgctttggt
    56041 aaggcattta caggaggtaa catgaaaaaa tgctttattt atcacgatga aaaatcgaat
    56101 aaattttggt ggatagatta cgaaggggat agtttagctg tcaactatgg caaggtaggt
    56161 agtattggta aattccatac aaaagaattc gataatgaag aacagtgtct gaaagaagcc
    56221 agtaaattga ttgccgcaaa aatgaaaaaa ggatatcaag aaactccaga ctttaacttc
    56281 atggatctct actattttga tgatgaagag attgggttac atcttaaaac gtcacaccca
    56341 aacttccagt gccattttac tgatccactt tatatgtgtt gctgggatga agaatctcct
    56401 tttggtagcg atgaaggtgc tgatgctcta aacgaacttg aaaatagcct ccgttctgga
    56461 ctgtgctgat ttccctcaaa tgttaattga aactatgtgg ggtatgaaat acatcgctat
    56521 ggacagtatt cttgaagagg atgtgcgtgc gcaattacta gccgatgaaa tgagctctat
    56581 ccagagcaat atgattacct acgcgactgc attcggtcag atcaaagtca tgggtaaaat
    56641 ctcccataaa cttaaaaaga tgggactcaa tgcactagcg cgtcatcagc ttaccgcaaa
    56701 aattcttcaa tggggtgacg gtcaggactc accaatactt caaaaaatga ttgatgacct
    56761 tactgctttt ccacacgaaa attaaaaact gcatttgtcg gcagcaacaa ctgccgacaa
    56821 atcagattat tgacaaagtg cactttgttt atgccggatg cggcgtaaac gcctgatccg
    56881 gcataaaaat attgctaatt caatatattg cagaaacatt gtaggcctga taagcggagc
    56941 gcatcaggca gttttgcgtt tgtcatcagt ctccaatgct attaatcctt aaatccctgc
    57001 cccctgacta aaatgctctt ccccaaacac tccggtggag aggtagcgat cgccacgatc
    57061 gcagatgatc gctaccacca ccgcaccagg gttagctttt gccacccgca gtgctccagc
    57121 aaccgcgccg cctgagctga cgccacagaa tattccttcc cgcaccgcca gttcgcgcat
    57181 ggtgttttcc gcatcgcgct gatgaatatc cagcacctca tccaccagcg cagcgttgaa
    57241 aatccccggc agatattccg caggccagcg gcgaatgccc ggaatactgc tgccttcttc
    57301 cggttgcagg ccaacaatgg tcaccggttt ggattgttcg cgcataaagc atgaaacgcc
    57361 ggtgatagtg ccggtcgtcc ccatgctgga gacaaaatga gtgatgcgcc cgccggtttg
    57421 ctgccagatt tccggcccgg tggtggtgta atgcgcataa gggttatcgg gattattgaa
    57481 ctgatcgagc agctttcctt cgccacgatt cgccatctcc agcgccagat cgcgcgcacc
    57541 ttccatgccc tgctctttgg tgacaagaat cagttccgca ccataagcac gcatcgccgc
    57601 acggcgttcc tggctcatgt tgtcgggcat cagcaatttc atgcgatagc ctttcagcgc
    57661 ggcaatcatt gccagcgcaa tgccggtgtt accactggtg gcttcgatta agacatcacc
    57721 cggtttaatt tccccgcgct tttccgcctc gacgatcatc gaaagtgccg cacgatcttt
    57781 caccgaacct gccgggttat tgccttccag ttttaaccac acttcactgc cgttatccgg
    57841 ccccattcgc tgcaacttca ccagaggcgt attgcctatt gtttgttcta atgtactcac
    57901 aatctctatc catacgtggt gttgcctgat gcgacgcttg cgcgtcttat caggcctaca
    57961 ggttacaaac cttgccatta aaaagcccgg atcgcgggaa gcgcctccgg gcgtttaaca
    58021 ttcactcaac ctatcaggcg ctttgtgcga gagcaagttc ctcatcgcgg gtttcaatac
    58081 gctcgtcgcc gttatacagc cgcgcatgtt gcagaccaac gaataaacgc tcgccatgct
    58141 gcggggcatc gtcgccatgc atcacgaccg tcagcggttc gttgtaccac cccagcggct
    58201 gcaccactaa ttgggtgtag tgacctttcg ggctggcttc cagtacctgt accggcagcg
    58261 gcgaatcgag actggtacgg cggctgatat ccacttccca cgggcgcagg aagagatcca
    58321 ccggcccctg atacgcaggt gtgtagccca gcggccagcg atgcgcgcca acatggaact
    58381 gcccgccgcg aatggttccc tgcaggcggt tcacttcgcc cataaattcg agtacaaaac
    58441 gggtcgctgg ttcgcgccat acctgattcg gcgcatcagc ctgttcaata ttcccctgac
    58501 tcatcaccac tacacgatcg gctacttcgg tcgcctcttc ctggtcgtgg gtcacaaaca
    58561 cgctggtgaa tttcagctct tcatgcagtt gacgcagcca gcgacgcagc tctttacgca
    58621 cctgcgcatc cagcgcgccg aacggttcat caagcagcag aatttgcggt tctacagcca
    58681 gcgcgcgcgc cagcgccaca cgctgtttct ggccgccgga aagctgcgcc ggataacgat
    58741 ccgccagatg cgcaagctgg accatttcca gcaattttgt cactttcgct ttgatggctg
    58801 cggcattcgg gcgctcgcga cgcggcagca ccgtcaggcc aaaagtgata ttgtcgaata
    58861 ccgtcatatg gcggaacagc gcgtaatgct ggaacacgaa accgacttta cgatcgcgcg
    58921 cgtgcaggcg actcacgtcg gtgccgtgga agcgaatatg cccgctggtt tgatgctcca
    58981 gcccggcgat aatccgcagc agcgtggttt tcccggaacc ggacggcccc agcaacgcga
    59041 ccatctgacc agaaggaata tccagtgaga tatcgttcag cacctgggtg cgaccaaaag
    59101 acttcttgat attggcaatc tcaatgctca tgatgctcct cctgctgcgc gcgtttttcc
    59161 tgattctcca ggcgccactg caacatactc tttaaaaaca gggtgataat cgccatcaac
    59221 gtcaacagcg cggcggcggt aaaggagccg acggtgttgt agtcctgctc caacaattca
    59281 atctgtaacg gcagcgaaag tgtttcgccg cgaatcgagc cggaaaccac cgacactgca
    59341 ccaaactcgc caatcgcgcg ggcgttggtc agcaccacgc cgtaaagcag cgcccagcga
    59401 atattcggca atgtgacgcg acggaacatc tgccagccgg acgcgccaag caaaatcgcc
    59461 gcttcgtctt cctggctgcc ctggcttaac atcaccggca ccagctcgcg caccacaaac
    59521 ggacacgtca caaagatggt gaccagcacc attccgggcc aggagaacat aatttgcaga
    59581 ttatgttcat ccagccagcc gccgagcggg ccgttggagc cgtagaacag cagatacacc
    59641 agaccggcaa ccaccggcga tacagcaaac ggaatgtcca gtagcgtcag cagtaactgg
    59701 cgtcccggga agttaaagcg cgtcaccagc caggccagca gaatgccgaa caccaggttt
    59761 accggtacgg caatcagcgc gatcatcacc gtcagccaga tggcgtgcag catgtccgga
    59821 tcggccagat tctgtaaaac cggcatcagc cccttgctga atgcctgcac gaagatgtaa
    59881 atcatcggca ccagcaggat gaacgccgaa accagcatcc cgatgccaat cagaaaccat
    59941 ttgccccagt taatcgggcg cgcgtcataa cgcttcaatt gggtaacttc cgccattaat
    60001 gacctaccac acgccgacca aagcgacttt gcagagtgtt aattgagaac agcagcagca
    60061 gagatgccgc gaggatcacc gaagcaatcg cgctcgctgc cgggtaatca aactcctgta
    60121 agcgcacaaa aatcatcagc gacgtcactt ccgtcttcca cgcgatattt ccggcgataa
    60181 aaatcaccgc gccaaattca ccaagactac gggtaaacga cagcgccacg cccgccacca
    60241 gcgccggaga aagctccggc agcaccactt tgcagaaact ctgccagcgc gttgcaccaa
    60301 gcgtttccgc cgcttcttca tattccgggc ctaactcttc cagcaccggc tgcacggtac
    60361 gcaccacaaa cggaatgctg gtaaaggcca tcgccaccgc aataccaagc caggtatagg
    60421 tgactttgat atcaaacttc gccagccatt caccgtaaaa accgtttacg gaaaagagcg
    60481 aggccagcgt taaaccggcg actgccgttg gcagcgcaaa gggtaaatcc atcagcgcat
    60541 caagcagcgt gcggcccgga aagcgataac gtgttaaaat ccacgccatc agcagaccga
    60601 aaacgccgtt aaaaatcgat gccacaaacg ccgacagcag cgtcactttg taggccgcga
    60661 ccacctgcgg gttggtgatc acctcccagt actgcgccca gctcatctca gaaagctgca
    60721 tcaccagcgc agagagcggc agcagcaata tcaggcacac aaacagcaga ctggtgccaa
    60781 ggcttaaagt aaagcccggc agcacgcgtc ttgaagaaac tgcaaacatt acttacgccc
    60841 cgccgctaac agcttgtcta actcgccgcc gctggtgaag tgggttttca tcacttccgg
    60901 ccatgagcca aatttgtctt ccacgcggaa cagttcggtc tgcgggaatt tatctttcag
    60961 tttgtccatg acttccgggt tattcacacg gtagtaatag tcggtgatga tggtctgcgc
    61021 ctgtgggctg tagagccagt tcaggtaggc ttttgcggct ttttccgtac cgttggcctg
    61081 cacattttta tcgacccacg ccaccgggaa ttccgccaga atattggttt tcgggatcac
    61141 cacttcaaag ccctgtgctt catactgttt acggatgttg ttcacttccg attcaaagct
    61201 aatcagcaca tcgcccaagc cacgctcggc aaaagtggtg gttgcgccac gaccgccggt
    61261 atcgaacact tcaacgtttt tcaggaactg ggtcataaac tgttcggttt tggctttatc
    61321 gccaccgtca gctttatccg ctgcgcccca tgccgccaga taggtataac gcgcgttacc
    61381 cgacgttttc gggttcggga aaatcagctt cacgtcggag cgcaccaggt cgttccagtc
    61441 gtggatattc ttcgggttgc ccttacgcac caggaagccc atggtggagt agaacggcga
    61501 gctattattc ggcaggcgcg actgccagtc agccgggatc agcttgcctt tgtcgtgcag
    61561 gatttgtacg tcggtcacct ggttataagt gacaacgtct gcttttaagc cctgcaaaat
    61621 cgccagcgcc tgttttgatg acccggcatg agattgtttt atcgtcagtt tgtcgccgcc
    61681 gttatctttt gcccattgct gttcaaacgg cggattcagg gcggcaaaca gctcgcggga
    61741 gacgtcataa gaactgttca gcagttccgt tgcctgtaca tggcccgcca gcagcagaga
    61801 agcgaccagc gcgagtgagt tctttttcag taagttaacg gccattgcgc acccttataa
    61861 atttaatgac tttctaatag ccatcatatt tataacggac gtgaaaggag taacggtttt
    61921 atataccgtt tggtgatttg gaagttaaaa agggaataag aacttggttc aacaggcgta
    61981 attgttgcag gcagtttgga cacggacagc gcggagaaac cggagcgtac acatagtacg
    62041 tgaggatttc gagcactgcc cggggccaaa atgacaaatg aaatagcctg gtgaacttag
    62101 ttcaagacga aatcctcccc acacatgcag ggaggaaaca gaggtgaatc agataccgac
    62161 gctaaccgtc tccggcagtg tgctgccgcc atcaatcaca ttctgtgtac cggttaaata
    62221 gctggattca tccgatgcga ggaaggccgc cagttcgccg acttccagcg gatcggcgag
    62281 gcgacacagc gggattgctt ttgccatttc agtcagcacc gattctggat cttccgggtt
    62341 cgactggcgg gcaatgcttt ccgccattgg cgtgcggacg tatcccgggc agatggcgtt
    62401 aacgcgaata ccggattgcg cgtactccac cgccagcgat ttagtcaggc caacaatcgc
    62461 cgctttcgtt aaggcatacg ccgtttcgcc aggatcggcc accatatcac cagtgactga
    62521 agacatcatc acaatgcgac catctttgcg cgcaatcatc tccggcagca ccgccttcgt
    62581 gacgttccat acgcctttaa tattgatatc aatatggaaa tcgcgatctt catcgctcat
    62641 atcgaggaaa ctgcccagac gacaaacgcc tgcgttattc accaggatat caatgcgccc
    62701 ttctttttcc ttcgcgcgtt tgatagctgc ggctaccgac gccgggtcac gcacatcggc
    62761 gacaaccgcc gtacagcgat gaccacgacc acacagttcg tccgctagct tttcgatctc
    62821 aggggagata tccagcaaga ttaggttcgc gccatgacgt gcaaaagttc tggcaattcc
    62881 ttcgccaatt ccctgcaatg cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat
    62941 tttaatgctc tccttgttaa tacagagcgt tacactccat tccctctgaa aatacaggat
    63001 aaacaaaata ttgctgcgga tcagctcact aattcaggaa aatctcaatc cagtcgctgc
    63061 tttatgcatc atttcaggcg ttgtgttagc tcactgctgc gctgaatcat tttcaatgac
    63121 gccacatcat tgatgttgca caagaccaac aaacagtaaa tcggtaacag aattttgcgc
    63181 cgtgcgggta gacatcgacg agctacgcca ttcggtttca ccggaaacgg tatcgagcgt
    63241 gaaatgcgcc agccgccgca gcggagagtc agccagagaa gtaatggcaa tcaccgttgc
    63301 cccctgcttt cgtgccgctt ctgcgcacaa gacaatctct tttttactgc cgctgtaaga
    63361 gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt
    63421 gtcggcttcg caggccacgc gatagccaat tttcatcagt ttgaacgaca gatcgcgccc
    63481 caccagcgcc gatccgccca ggcccgttat ctggataaac ggtgctttac tgatgacctc
    63541 aatgattttt tgcagccgcg cgtagtcgaa caacgcacag gtttgctcca gcgccagctc
    63601 tttttcgcga ttcagtttgc gggcaatcac ttccagcgaa tcatcactgg taatcgaact
    63661 gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg gcactgtatt cgccaattaa
    63721 cgccatacgc aattcagtaa atccttgcgc gccgagcttc tgggcaaatt tcacaatgct
    63781 cgactggctg ataccgagct gtttcgccat ctgccgtgaa gagacggatt tcagctcgct
    63841 cactctggcc cgcagaaagt cagcaatttt ttgctcgttc ccggtaaatt cactttccgc
    63901 attgcgtatt ttcgtcaggt acaacatctt ccactctcct gggctggcag caaaaattcc
    63961 gcttaagttc ttatgacgct cttcacactc tgcgagtgta gactcaatga ttcctttaag
    64021 tctgtcaatt cgggatatat gattccatac atctgcaatt atttgatcgt aaatagtaag
    64081 gtcaccaccg atgcaactag aaaagatgat tactgaaggc tcgaacaccg cctcggctga
    64141 aattgaccgc gtatcgacgc tggaaatgtg ccggattatc aacgatgaag ataaaaccgt
    64201 accgcctgcc gttgatcgcg tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc
    64261 ccaggtcagc ggcggcggtc gtctgattta tctcggtgcg ggaacatccg gtcgtctggg
    64321 gattctggat gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg
    64381 tttgattgct ggaggcgaat atgccattca gcacgcggtg gaaggagctg aagatagccg
    64441 ggaaggtggt gttaacgatc tgaaaaatat taatttaacg gcacaggatg tggtggttgg
    64501 cattgctgcc agcggtcgca cgccgtatgt gattgccgga ctggaatacg cacgccagct
    64561 cggctgccgc acagtgggaa tttcctgtaa tccggggagc gccgtttcaa ccaccgctga
    64621 gtttgccatt acaccgattg taggtgccga agttgttacc ggttcttcgc ggatgaaagc
    64681 aggtacagcg cagaaactgg tgctcaatat gctttccacc gggctgatga ttaaatccgg
    64741 caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg
    64801 ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt
    64861 aattgcctgc gagcgcaact gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc
    64921 cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg ttttagacaa
    64981 ggaataaccc atggccaaag agatcagcag tgaacttctg aacaccattc ttacccgtgt
    65041 cggcggaccg ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt
    65101 acatgacagt tcactggttg atcccaatat caaaacgctg gaaggcgtga agggcgtcat
    65161 tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa
    65221 agcgatgagc gagctgctgg gcgatgcacc ggtacaggat gccgcagaaa tcgccgccca
    65281 gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt
    65341 cgccaccatc ttcacgccgc tgatccccgg ttttattgcc gccggtctgc tgctggggat
    65401 agcgacgtta attgccacgg tgatgcacgt tccggcagat gctcagggca cgctacccga
    65461 cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc accttcctag tgattctggt
    65521 gggctataac gccgcccagg catttggcgg cacgggcgtc aatggcgcaa ttattgccgc
    65581 actctttttg ctcggttata accccgctgc aaccaccggt tactacgccg gttttcacga
    65641 tttctttggt ctgcccatcg atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg
    65701 ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg ccggacgatc tcgacatgct
    65761 gctgacctcg ttaatcaccc tgctgatcac cgctacgctt gcgtacctga tcattatgcc
    65821 gctgggtggc tggttattcg aaggtatgtc atggctgttt atgcacctga acagtaatcc
    65881 gttcggttgt gcggttttag ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca
    65941 gggctttatt cctgtttacc tcgcgttaat ggacagccag ggattcaaca gcttatttcc
    66001 catcctttca atggcaggcg cgggccaggt gggcgcggcg ctggcactct actggcgggc
    66061 gcaaccgcac agtgcgctac gcagtcaggt acgcggggcg attattcccg gcctgctggg
    66121 cgttggtgaa ccgctgattt acggtgtcac cctgccccgc atgaagccgt ttgttaccgc
    66181 ctgtttaggc ggcgcggcgg gcggtttgtt tatcggattg atagcctggt ggggtctgcc
    66241 gatgggctta aacagcgcct ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc
    66301 cgcacaaggc atccttccgg caatggcggt ttatgctggc gggattctgg tggcatgggt
    66361 ttgcgggttt attttcacca cgctctttgg ctgccgtaac gtcaatctgg actgatatta
    66421 tgaaacggac aatgctctat ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa
    66481 aataccctgt tctgacagaa ggtagttgat caagtatatg cggcgttaaa gcagtaataa
    66541 aaaggccagt cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag
    66601 cgccatcaac ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa
    66661 acgcaacatc gcatcacgtt tgccatcggt atcgccaaac atgctcagca gttgctgctc
    66721 aatgttatgc agacgcgcgc agtaggcgca gaagtacaga ccgtgagtgc cactggcggt
    66781 gccgtacggc aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac
    66841 gcgggtgagg tgagaggttt ccggacgttc gtcgccgtca atctcttcgt tggcctcttt
    66901 ggtgcgcccg atcatcatct cctgatcgtg aacgctcatc cggttgagct gcttcaggtt
    66961 gtgctcccaa cgctgcacaa acacatagct gccgccggaa tccacgccgt ctttgataac
    67021 cgccacttcg cggcgcgtct cttcacccgc cgggttttct gtaccgtcaa caaagccgct
    67081 caggtcacgc tcttccaccc aacggaagcc gtggatctct tctttcactt caatgcagtc
    67141 accaaaggct tccatcgccg cctgagcgac agagaagttt acgtcgtgac gcagagaaag
    67201 aatgtggatc aacacatcga actgggtcgt cggcgcaagg cctttaccgt agcccggaaa
    67261 atctttcagc tcttctgccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa
    67321 ggcaaccacc gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc
    67381 ggcaaatgtt ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat
    67441 ccaaatcgcc gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc
    67501 tcctgaaaat aagaatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt
    67561 ttcgcgacgg cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc
    67621 cagtttttca gggtatcgtc cgaaggcatt aacccttcag gaccacgcca ttccccgctg
    67681 aactggtagc taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca
    67741 ccatcagctt tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg
    67801 attttaacac cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg
    67861 ccttgatcgc cgttaatcac catcgccacg ttgtcgcctt tcatcacttc aaaaaagcgc
    67921 accacgttgc cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca
    67981 tcagcaatgg cttgttcttg cagtggtgtg gacgccgtta attcacccac gccctgctcg
    68041 ctcactttgg tggacgatcc aaaccagttc cacgggttag cggcagacca gttaactgac
    68101 gacatcgtgg aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcagcgat
    68161 ttcatgtcac ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag
    68221 tgccgttatt ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag
    68281 attaacgcca caatatccgc cactaccagc gccagaccga taccgctaac ggattcaccg
    68341 ttcagccaca gccacggttg ccagcaaagt aaaaccacct gcgccaacag caacagaaaa
    68401 tagagcacat gccaggtgcg aggaaacgaa gcccgccgac cgctcaacaa aaacgccagc
    68461 accgccggaa taccgggaat cagccccagc cagaaattat cgtgatcggg ataaaacaga
    68521 tttagcagcg cagtaccctg ctcacgcgac gcaccggcaa tgacaaacag cacccaggtt
    68581 cgcgcctgaa gcaatagcac aagccagaag agcaagggta aacgcaggcg tccgtgcgca
    68641 tcataatgga caggatggaa ctcagtactc ttcatcttca atcaaacgct tacccagact
    68701 cagcacgtcg gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc
    68761 ttccggcacg ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccagccg
    68821 gttaagcaac gcattggcaa ttccacgccc acgaaactct ggatgcacgc caagataata
    68881 agcagacccg cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgtttac
    68941 ctcagcgacc aaaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg
    69001 atcgttccac ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc
    69061 ttcctggcga aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct
    69121 gattatggcg tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc
    69181 ataatgggga gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaaccgt
    69241 cgtaacggat tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt
    69301 atgagcactt ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt
    69361 ttagctgccc tgacgttgtc aggaatgtcg caagccattg ccaaagacga acctttaaaa
    69421 accagcaacg gacacagcaa gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt
    69481 ctcgatccag gccacggcgg aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa
    69541 aaacatgtgg tgctggcgat tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt
    69601 gatgcgcgtt taacgcgttc tggcgatacg tttatcccac tttacgatcg cgttgaaatc
    69661 gcccataaac atggcgcaga tctgtttatg tcaattcatg ccgatggctt taccaacccg
    69721 aaagctgccg gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg
    69781 aaatacctgt ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac
    69841 aaggatcacc tattgcagca agtactgttt gatctggtgc aaaccgatac tattaaaaac
    69901 agcctgacgc ttggctcgca tattctgaaa aagattaagc cggtgcataa actgcacagc
    69961 cgcaacaccg aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg
    70021 gtggaaacct cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcgttt
    70081 cgtcagaaaa tcgccacagc gattgctgaa ggcgtgatca gttatttcca ctggttcgac
    70141 aaccagaaag cacattcgaa aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa
    70201 cagtttctgc tcaaccttca ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca
    70261 gaatttgtcg aagatagttg gcagcgcgaa gctggcggcg gcgggcgcag tcgggtgttg
    70321 cgtaatggtg gtgttttcga acaggcgggc gtcaactttt ctcacgtcca cggtgaggcg
    70381 atgcctgctt ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg
    70441 ggcgtttcac tggtagtgca tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg
    70501 cgttttttta ttgccgaaaa accgggtgca gagcccgttt ggtggtttgg cggcggcttc
    70561 gatttaaccc ctttctatgg ttttgaagaa gacgccattc actggcaccg caccgcccgt
    70621 gacctgtgcc agccatttgg tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat
    70681 tacttctacc tcaaacatcg caacgaacag cgcggcattg gcgggctgtt ctttgatgat
    70741 ctgaacacgc cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac
    70801 accgacgctt atttaccaat tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag
    70861 cgcaattttc agctctaccg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc
    70921 ggcacgctgt ttggcctgca aactggcggg cgcaccgagt cgatcctgat gtcaatgccg
    70981 ccactggtac gctgggagta tgattatcag ccaaaagatg gcagcccaga agcggcgtta
    71041 agtgagttta ttaaggtcag ggattgggtg taactccctc acccccattc ccgcatccgc
    71101 tgatgcaacg tcaacgactg cttctcggca aacagctgct ggtaatccgt ggcaaattgc
    71161 cccagatgcc agaatcccca ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac
    71221 gggcttatca gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca cgcgtttggg
    71281 ccaatgccta aaatagcgtg aaacgcgttt tgtagcgtgc ggcgactgac atgcagttga
    71341 ttacacaaat ccagcaccgt caccggttcg gacatgtttt ccagcacata ttcacgggcg
    71401 cgggaaagca accgacggta actctgatga ctgatgcttt ccgccgtcac cattggttgc
    71461 gcttcttcca gcatcgcccc catcgccatt agcaaattat cccccagcac ttttcgcact
    71521 gcaggctgat ggagattttc cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag
    71581 ccccacagcg cggctttatg ctgctctttc acttccagcg ccgactggct acgcagcata
    71641 tgtaataccc gatccggatt gtgcaaaaag ttagcctgac aggtgatgac atcttcagac
    71701 agcactacgc ccaggatcgt ataatcgtcc ggcgtactta attcaaactc ggttccaccg
    71761 gggcgcgtcg ctatttctgc gcttcccaga cattgcgaac cgataaatcc ctgctcaccg
    71821 cgcgtcgccg gaatgccaaa ccagaacgag ttcggccaga ccaggcacga ctgacgcagc
    71881 gccagaccgg tgtattcacg aaaaacctga atatcatcga gtagaatttc cgtaaattca
    71941 ccatgaaact tgcccggatg cagctgatcg taaatctgct gctaggcggt aatcgtcaaa
    72001 gcatgttcat agacatccgt tgtctgtcgt tgatgaacat tatccacttc gaccttcggc
    72061 gtgagcttca ggttttcggg taagggttca tgataaagat ggtgcaaatt ggctgtacgg
    72121 gtctttttca tgatgttaat gccgggtgtt gtaggacacc cggcacctcc gacaggttaa
    72181 tggggcttga gacgataacg actactgcgt ttacgtaacg tcccggcaga aaagagctgt
    72241 tcgagcgcat ttctggcttt ttccagcggc cagccaaagt gggcggccac ttctcctgcc
    72301 gtcattccct gacatactga tgttaacagc gccaacaact cgtcagcaga ttccgcgacg
    72361 ataactggcg cgtcgggttc ctttacgggt tgcttcggct ggcggttaaa gccagtaacc
    72421 agccactggg tgtcatcgtc tggccgaccg atctccttac ggctgatgac acgcccggta
    72481 cgcatcgcgg cagcacaacc tgcgtccagc gccgcacgac acgccgccag atcgccttcc
    72541 accaccagcg ttaagcgacc agggtcaagc acttcgtgac tgagcagacg aacgttagct
    72601 gctttgagca tggcatccgc tgcatctatc gcggcgacca tgccgtccac ttccagcaat
    72661 cccagtgcat tgatcatcgg caacctccgt tacgcacgct ggattggatt acgcgcgatt
    72721 tccagcactg catcggtaaa ggcgttacag gctgctttac acgcggcctg gctaccggtt
    72781 aaaaatgctg ccgagtagtt ggtttcagac ggtggcggga cgtaggtcac cagctgtacg
    72841 tcggcagatt tcaacgcggc atcaatgccg taggtcgctt ccagcggcgg tgccaccaga
    72901 tacgccatcg gatcgccaag cgtgatcccg gcggttgatg aaagataaga accggtacgc
    72961 gaaaccacat gcgccaggaa ggcggtgttt tctgcgtcat tggcccactg gaaagctgcg
    73021 ccattttcaa tatgtgcaac catcgcatcc agaccggcac gcacttccgc cgggtttggc
    73081 ccaccaagca taatcagcac ttcaccggca gtcggtgacg ggccgtgtgc cgctcccgca
    73141 tacagcgagc gaccatatac cacttcgacc atcgcttgtt tggtcgcttc gtcagccgca
    73201 atgtaggtta cgtcgtcaga atcggcagaa atcagcccga ggctacgaat atgcggcggc
    73261 aattttagtt cacgcgcaaa ttcagcgtta acggaggcaa tcacccgcat ggcggttacc
    73321 gacggtcgaa tcaaatctaa agctggcatg atgcctcctt atcgggtcat gttgatgccg
    73381 gatgctttct gctccagcat acgtttcgcc aaatccacaa tgacggcaca gcagcacgaa
    73441 agagaagcaa aagtcagcta cctggtacac taggcagcgg gtcagcaaag gggggcaaat
    73501 gccccttttt tcatgtcaga taatcagatc attacctcaa taaactaata atcacatcgc
    73561 ggttacaacc agtttgatag tgatagctca aacccagacg tggcgctggc tgcaagcaaa
    73621 ataagccgca aaaatgcgac cccatgttta ctagccgcaa aaatgcggtc ctatttgcca
    73681 gaacctttta ttattcaatt ggttagtgca agaaaaagcg tgattactgt tcaagaagta
    73741 tccagaaagg tcatgatttt gacgctgaaa aaaaataggt gggcattttt gcccactcac
    73801 tagccgcaaa tttgcccact cactagccgc atttttgcgg cccaaagtaa taaatacaga
    73861 gaaataataa atacagatca gcgccaagat ttttcgtctt ttcggcggaa tcatgagact
    73921 agtgcttacg tcctgataac ctccagagca gaccttaact tctgtgcagc aggactacgc
    73981 tcattgccgc tcggctaggt caaatcctca cgataaagct gttcggattg tgaccataat
    74041 gttaaaggac tctttaaatc attccttgac gaattaacta gctggcttaa gcggaatagc
    74101 atgcaggata aaaacaaaac cagcttagca gtggggagag cttcgcttcg ctcgtctcct
    74161 ggggccaatg ctcaatgaaa cgacgtaagg cacggagcgc tcaccatagg tagacacata
    74221 aacatctgag agcgcacctt ctatcgcgct acgcgctcaa taaaaaccaa cgacaccgcc
    74281 acactgcaag accaatggtt tttatcgcgt atacagacca gacaggaaac agcagcaaag
    74341 cagcgtatag ccaggtgcag acagcacact cacacgacgc acagcaggct taagtgatca
    74401 ctcaggaaac tttatccccg ccgccgatca ccgcgaacta tcatgaccgc gccaaattcg
    74461 aaaggaatgg cccatagaac gcgatgaaca cacataagcg ctacacttac ttaacgtcgc
    74521 cacagagcga ttgagagcgc ttaaaatcga tgcgatttca ttaaattttt ctcactcgcc
    74581 tgaatggtgg cgatcaggac tgatcgcaac attgccccgc ctcgatattg agcgcataat
    74641 agatacatac attaagcgga aaagagataa gtttattgtg aaaatcaggg ggttacatat
    74701 catgcgcata tcagaacaac aacggcagat cctcgactat ctggcaaaaa acgaatcaat
    74761 caccgcacga caagccgcag atctcgttta tggtggcaat gttacccgcg tccaggttga
    74821 cgccgcccgc cgctcactca tgacgatggt tagtaatggg ctaatcagga aacaaggccg
    74881 cgctttcgta gctgaaagac atatcaatca cgcttatagc caggacgttg aagaattgcg
    74941 ccgccagatc ctttgggtat tcagcaaaca gggtcaggag tatttccttt ctgaacaccc
    75001 aaatttcgcc gccaaagagt taacagtaag cggattgtcg tgcgcactgg attatcttgt
    75061 cggtgaaggt tgcgttactt acaagcagct atggcacgcc ctgaatacgc ttgtagaaga
    75121 tggtttactc attgccacgc gtgagaacat cgaatactgg ataatgccgc gttcaggtcg
    75181 ccgcaatcat gcacggtata tttcaggcaa cagatacaga acgccggact ttaatccgac
    75241 acaggaagat cgggaccgtg ccgaaatgat gtgcagggaa caggagaaaa gaattatgga
    75301 taagttattt tcgatgcagt gaacacaagc gggcgcaatg cccgttttta ttgccttatt
    75361 ccacgcctga cgaatcaatt tgatcacacc tgagaaaatg catagccaga agttaaggcc
    75421 gttatattgc gattgggagc gttttaaacg gtatcaattt aattaattct gatgtggtga
    75481 tttgcaggca aaaaaaaccc gccggagaag cgggaaatag agacatatcg ttgtagttat
    75541 taacagatca ctttgaggtc gagggcggct aaccctcgct aatattatcc cgctattacc
    75601 ttgcgggtgc aaataatctt ttattgcttt gatgatttaa taaatagcac tttttgctaa
    75661 caaaaactgc tgtcaatata tggccaaaaa tgtcaacaga aaaaataagg tttataatat
    75721 taagtatcac atgggtaatg ggttcaggga atcggaatcc accgcctcac ggaaattcaa
    75781 aatttgcttc ttctttcctc cgtgggcggc ttctttttcg tatcgaggta tttatgaaag
    75841 caataacatt agaacagttc gcagcaatgg cgaatgtatc gaccagaacg ctgaggcgct
    75901 ggctaaaaaa accatcaacc ggagtccctt tgccatttac cccacgcggt actcgccaat
    75961 tattctggga gcacgaatgc aaagaatggt tgtacagaac agaacgataa taatcacgca
    76021 tcacattttg aggtaaacat catgaccaat atcgaaagat tagaacgctg tgccgatatt
    76081 atgcggcgca gatggattta tgaccctgaa gcgggtttat tgtttagccg tgaaacaaaa
    76141 gacgttatca aaggcagcat gaccaataaa gggcatttgg ttgtaacggt acatgaaggt
    76201 aaattcaaag tgacactcac ctatcagaaa gcctgctatg tatacacata cggcgcttat
    76261 gacgagacat tatatgaagt gcaccacgtt aatttgaaca aacaggataa ccgcatcaag
    76321 aatattcgcc tgatgccgaa agaaaagcac cgccggatac ataaaaacgt ccgcaaactt
    76381 attcggctgg gccacacaca atggcttcaa ccgcaattaa ttgaacaatc actataaaca
    76441 caggagtaaa taccatgcat tcattagaca gttattttca gcgcacgaca gcccctaaat
    76501 cagccgccca ggaacgccgt gaggaattcc atgagaaagt gatgcgttct gctgattata
    76561 ttgcggacaa gttcgtggaa acggttcgtc cacttgttga tgaagtagct gataaacttc
    76621 agtctgaaat gccggaagat atggaaggca ctgcaaagcg gcgactcatt tgcgaattaa
    76681 gtcgtcgttt tggcgtgtca ataagcgctt ttaaataatc gaaaaaaggg gggaaagata
    76741 atggacgcat ttgaggtact ggcaaataaa ggcgctgaac tggtagcaca gcgtgataaa
    76801 accgccaacg aaggggaacg cgcactgcta aataaacaga ttaaagcgat ccgtatggcc
    76861 cagtttaaat taatcagtaa tgaagttatc gaaacaatta aaccgcagat cccacaaatc
    76921 gttgctgacg ctattaaagc cgccggactg gagaaacgca ttgccgggct aaaaacgaac
    76981 gcagagaaaa tggagatgca caaacagagc ggtgctccga atcccgatga gtacttctca
    77041 ccggatgttg agtttatggc gcaggttgag gaacgtctgg gtagctgtct caccgaggaa
    77101 cagcgtcgct attttgatgg agtggatagt tcggcaggta ttgacctgaa ctcgtatttc
    77161 ggtcgtgaaa ttgagcattt tgatgccgcc ccgtctatgc cggagccaga accagaaatg
    77221 gccaccgatc ctgaagctga gcaacggaaa cgcgtattca atgcgatagg gattcggtac
    77281 tgatcggata aaagaaagcc gggaattacc ccggctgact taacaatgtg accaatgaac
    77341 aaatcggaca agacaaacag gataagcagt ctcgaaacaa tgttcgggaa ctatattagc
    77401 acaagtccgg agaaaatcat catgttggca ctacgcatca tgcagggcat cgctaagacc
    77461 ctcgcagaac atgtcttaga cctgaaacat tcgccactga gcaaacaggc catgaagcgc
    77521 caaacgcttc gtctgtgggc tgaatattcg cttggaacta tcaataaaat catcgacatg
    77581 aaatcggggc cgagcaacca gagcgcggag gaaatggagt tcatcaggcg tttaattctc
    77641 attcgcagag acattcatag ccagttacac agcgtgggaa tcgatattaa tgatgggacg
    77701 ggcgattaat tttcggacaa gtactatgcg cgcgcgagga gctgaacatg aagcaagata
    77761 ttgcagacag gctggagata ctcgaaggcc agcgggcaga agcgaaacaa ctacgtaagc
    77821 aggccagaag agcgcacagg aacaacgaag cagagttact aaccaaatac atcagtttca
    77881 ccaattactg catctacgag tgttacaagg aagatgcaga ggactggctg gattcattgc
    77941 cagaacagta ctgagcagaa taacaccacg tcatgttgtt ctgaggtgag catatcacct
    78001 ctgttcaggt ggccaaattt agtgtgccac ttcagcatca ccgttgaaat cacaataacg
    78061 atttttttgt aaatatgctc tcacatatgt aggaatagac caggttggca ttgaaacaca
    78121 tctaccatga ccataataaa gtaatttttt gatatgttta tggttttata ttaattaaaa
    78181 tatatctaaa attgatagtt ttaaaattgc aaaatccaga ttgtgtttgt aatgtttaat
    78241 ctgattgtat tgagtggcgt ttattatgaa aaataaatca ggtaattctt gttatagaga
    78301 agcagtcgat attcatagct cacttgagct ttcatttata ttgcataaat atcatttgcg
    78361 tgtagatgag aagaaatctc gaaatgtatt cagcgccata cgtgatgagc tcatccttga
    78421 tggggattca cgacaaaact tagctacttt ctgtcaaaca tggcttgatg atgaaatccg
    78481 tgagttgctt aatttagcta ttgataaaaa catgatttat aaagatgaat atccacaaat
    78541 ggcggaacct gaaggtcgct gtgtgcgtat gctttccgat tcgtggagtt tccccgattc
    78601 gcgacacacc ttaggttgct caaccattgg ttcatctgaa gctgctatgc tcggccgctt
    78661 ggcgttaaaa tggcagtggt gtaaaaaacg cgaagtacaa ggtaaatcaa ccgaacctga
    78721 tttgcgatcc tgtgcaaata tgctggcata aattctgccg ttactgtgat gtgaaattgt
    78781 gtgaaattcc acttgatgat catcacctga taatgaatgc taaagaggtt ttgaagcatg
    78841 tcgatgaaaa cactattggt gtcgttacga ctttaggtat tacttatttt tgtaaagcag
    78901 attgcgttag tgtttttaag ataagtgtta aagttaatat tagtttttat atttacatcg
    78961 taattatatg tttctatttt aaaaatgttc gagagtataa aaatgtctaa tgctaatatc
    79021 gcacccaaaa gtaagaaaat gacagtgggg acactcgcta tcatgaatat aactgctgta
    79081 gtaagtctac gatttatacc ttctgaagca gaatacggct taggtgccat tttttactat
    79141 acctttgctg caattatgtt tttagtacct gttgcctttg ttgcggctga gctagctaca
    79201 acatatccac aaaaaggggg ggcatttcga tggataggag aagcatttgg ggctcgctgg
    79261 ggttttgtag gaatgcttat gacctggcta caagtcatcc cctactttcc aacagtactg
    79321 acatttggtg cagtctcggt tgcatttatt gatgttgata tgggggtggc agaatcaatt
    79381 gccagtaata aatggtatat tttatttttc gtactatttg tttactgggg agctatttgt
    79441 gttgcattac gtggcgttgg tattttctcg caggtatcta agtggtgcgg tattataggg
    79501 acaattattc cagctattat ccttgttatt cttggtttct cctatctgtg gttcagcggt
    79561 aaaccagcgg tgatcccatt aggttggggt gatcttttcc ctaatatagc taacttccag
    79621 aatattgttc ttgccgccag catatttctt gcttatgcag ggatggaaat gaatgcggtc
    79681 catgttaacg atttagataa tccaactaaa aaatacccaa ttgcaattac catcgcttct
    79741 ataggtacta ttgcaatatt cctgctcagt actttagggg tcgcctttat tattcctaca
    79801 gataaaatta gtctaactca aagtttgttg gttgcctacg atatgctgtt cgaatgggct
    79861 ggagtgccat ggcttggctc tgtgatggca tttatgctag caattggcgt actgggtggg
    79921 gtggttactt ggattgcagg gcctaataca ggtgtattag cgatcgcgaa agctggttat
    79981 ttacctaagt ttttccaaaa aaccaatcgt catggaatgg ggcaccatct aatgtttgta
    80041 caaggtataa ttgtctcagt tctttctgtt acttttgtga taatgccttc agttcaagca
    80101 gcttttcaga ttttatcaca gttaacagtt atgctctatt tagttatgta tatgctcatg
    80161 ttcgctagtg ctatctatct acgacattct caacctgata caaaacgccc atatcgagcc
    80221 cctgctttat ttttctgggc aacacttgga ttcttagggt cttttctggc ttttgtatta
    80281 agttttattc cgcccgagca aatcccaact ggtagtccaa ccagctatgt tctttatttg
    80341 ttagtattag ttgccctatt tttattaatt ccaatattaa tttacgcctt ccgaaaatct
    80401 gactggaaag atccaaactc tgatttcgaa ccattttctt gggaaaaaag aactagtaat
    80461 taaattttcc ataagctaca aaagatgagg ttaaatatgt ttagtgctga atctccaatt
    80521 ggtgtaatag actcaggtgt aggtggattg acatcggtaa aggaaataat taatttgctt
    80581 cctggtgaag atattatcta ttgtggagat aatatgaatg caccttatgg caaccgttct
    80641 gccgatgata tcatcagttt gactaaaaaa atgctgactt ttttacagtc aaggaatgtc
    80701 aaactagttg ctgtcgcatg taatactata tcctcaactt tggaatccga agaatacagc
    80761 ggatatgcta agagctttcc gtttcctatc ttatccatca ttgaacctgc agtagaagat
    80821 gtgattcgcc agcaatataa aaatgttggt attatcgcta ctgagtttac tattaaaacg
    80881 gggtgtcaca aagagctaat aaaaaaattg aattcaacaa taaatgtatt tggagaacca
    80941 agcaaaaatc ttgcaatgtt aattgaggaa ggtaatctta atgctcctgc aatcttgaat
    81001 gacatcaaaa aacatgttaa ccatttaatt tccctccatc cagtaaatga aattattctc
    81061 ggctgtactc actatccaat tgttcagaat ttttttgaag cagttgcacc ggatataaaa
    81121 tttattaatc ctgcacatga tcaggctatc tctattaaaa atcacttggg acagctaaat
    81181 ttattaaact cctcaaatat aggaactttg caaataaata ctagtggaag tatggagatc
    81241 tacaaaacgg tactagggga attgagcatt acgaaacctc atacattcag tatcaggcag
    81301 ttttaaattt atccggtgca ccgtaaaatc ccgtccttct gaactgcacc ccaaaagttg
    81361 gacaccaact gagtaaggtg cattttatgg acaaacccag ttacatactg ggtagtgcat
    81421 ccaattagta gaacatgtgt ttttcgataa acgctccgat cactttttcg tggatctcaa
    81481 ctgagcgtga gaagcagatt gttttacgac ccaaccgctt aatgcgggtg cgtagcgtca
    81541 gattattacg ctcaatgcgt tgggtgaata ttttgccggt cagatgctta ttcttcggca
    81601 cctcccggcc atagctgccc cagtcatcgc tggtcagcat gccgatgttg aagggtgtaa
    81661 gcagtgccag tagctcccgg cacgtttgat cggttcgggg accaaaagtg taggccagta
    81721 caccgcctgt tttggtgttg tacgcgtacc cgagccaatg ttgccgggct ttactgccaa
    81781 cgtagctcca ttgctcatca agctcgcaga taaacgccac atcagcatgg gcaacaggcg
    81841 aagaagttat tcgctttggc gcgagttttt taaagtccgg atgacggtgt tactaccaat
    81901 tttcagtgtc ctggcggtat cgcgaacccc ggcaccatta aaggccattt cagttatcag
    81961 ctctttcata cccggcttac gtgcttgata agtataagtg aactgaaaca cacggtggca
    82021 gtcacggcag cgaagtctgt cacggccttt agggttctga ccatggcggt aaacctgagc
    82081 tgactgacaa cggggacaat gaatattaac gctggtcatg agataacctc aaaagcccgt
    82141 attatacatc agattcaact aattagaggc atcacccatc cagcattata aaacatacta
    82201 acaataacta aattataata atcacaactt acaatcaaat aataaatgca taagattaac
    82261 aatgattttt tcgggttata tttatctaaa aaagcagaat attatgtaac ttattatttt
    82321 ctaagcgaaa atttattgtg cggtcagact aacaaacttg cgtaccaccc gaagaacatg
    82381 cgaagattta agtattcttt cttttttggt ttttattttt cattttctct tttcttctct
    82441 ttccggttct gttctggttt tggttttttc ctttttattt ttattctttc ttcagggtta
    82501 tgctaaaaca ccaaaacatt tttcaccaca aaagcatcca atcatcttgc cagaccaatc
    82561 accttaaccg gatacactgc cggatataat cccactaaca cccagacacc aagcaccata
    82621 cctgataact accgtattca ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc
    82681 gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac aatcacttcg
    82741 tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acaacatgcc cagagcttta
    82801 ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa
    82861 tcgaccagcg cgttgatgac cgtcagaacc gcggcgtaac gcacaggcag cgaggcggga
    82921 agcgccagca cgtacacgtc agttttggga tcaagatcca gctgaagcag cgcctgttgc
    82981 caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc
    83041 agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg tgcacccgcg
    83101 ccaatcaccg tggagcgtac ggtttgcgcc ggaaactgca cattcatctc acgcaggcgc
    83161 ggatggtcat gcagcgccgt tgccagaagt gggccaatat cggcaaaaca gaacgggttg
    83221 gcgggctggt ggcgataaca ttcgcccacg cctccagaaa gcgtaatgat ttcgggcgta
    83281 acacctgcgg gcagcaaacc ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg
    83341 tcaatcactt cgacaatcag cgccgccatc cgccgagtaa cctgcaccag ctgcgcgccg
    83401 gtcagcgaac gggcgtcagt gcctgcaccg aagcactcat ccacaatcat ctgccccggt
    83461 ttatgagcgt aaaccacgcg cccctggctg tcggtttcca gcaggcgacc accgacgttg
    83521 agacaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg
    83581 ccgatatcga tattcagtac ccgacacagc cgttgttcag aaagggtttg cgccccggct
    83641 ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc
    83701 agcgactgtt tttccttcat cactttttcc atcagctgtg cgaccaggct ttcggtaaac
    83761 tggccttccg gcagctgggc aatgatggtt tcgcggatgc gctggctttc ggttttgtcg
    83821 gctggcacgc tggcgggcac ggacgccgtt accgggatcg attcatcaca ttcggtgatg
    83881 gtaaaaccca gtagatccgc cacttcacgg gcttccgggg taataatgct ggcgcgcaga
    83941 accacggaca ttgcctgttc gccgcgcgcg tgtgcttcac gaatatcatt cgctgtgatg
    84001 agttttttca cctgcttcct cctgtttggt gagagatgcc agataatcca ccagttgctg
    84061 cacgctttgc gggtcgtggc tgtttagctc gaaaatcggc tcttcgaaac ccgtctcaag
    84121 caataacttt cgcgttgcgg cgacgtcagc atctggcatg tccgttttac tgatgacggc
    84181 gatttgccgt ttgctgacgc caatatccag caacccggca ggcaaccgac tttccgggtc
    84241 atttgcgcca tggacataga tcagcatgtc tacatcctgt agcgtggtaa ttaaggcgtg
    84301 ataccagcgg ggatggctaa aatattcacc cggcgtatca atatcgccct tatcattaaa
    84361 ttccacggcc agtgtttttc tggcgagggt ataattcccc tgcaacgcat taaataacgt
    84421 tgtttttcct gccccgacgc tacccacaaa cgcaattcgt ttcatgaatg cctcggtaat
    84481 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatgcag
    84541 ctccaccgat tt
//
LOCUS       DAJTNW010000003        78784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNW010000003 DAJTNW010000000
VERSION     DAJTNW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..228
                     /locus_tag="OO179_000177"
     CDS             <1..228
                     /locus_tag="OO179_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5530390.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OO179_000178"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OO179_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="OO179_000179"
     CDS             1366..1689
                     /locus_tag="OO179_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5530391.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO179_000180"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO179_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5530392.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO179_000181"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO179_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5530393.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO179_000182"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO179_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5530394.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO179_000183"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO179_000183"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5530395.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO179_000184"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO179_000184"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5530396.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO179_000185"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO179_000185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5530397.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO179_000186"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO179_000186"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5530398.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO179_000187"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO179_000187"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5530399.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQHTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO179_000188"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO179_000188"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5530400.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO179_000189"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO179_000189"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5530401.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO179_000190"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO179_000190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5530402.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO179_000191"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO179_000191"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5530403.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO179_000192"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO179_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5530404.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO179_000193"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO179_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5530405.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OO179_000194"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OO179_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5530406.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OO179_000195"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OO179_000195"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5530407.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OO179_000196"
     CDS             complement(21228..23060)
                     /locus_tag="OO179_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5530408.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO179_000197"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO179_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5530409.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OO179_000198"
     CDS             complement(23808..24302)
                     /locus_tag="OO179_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5530410.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OO179_000199"
     CDS             complement(24385..24840)
                     /locus_tag="OO179_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5530411.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OO179_000200"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OO179_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5530412.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OO179_000201"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OO179_000201"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5530413.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OO179_000202"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OO179_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5530414.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO179_000203"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO179_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5530415.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO179_000204"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO179_000204"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5530416.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO179_000205"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO179_000205"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5530417.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OO179_000206"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OO179_000206"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5530418.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OO179_000207"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OO179_000207"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5530419.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OO179_000208"
     CDS             33416..34309
                     /locus_tag="OO179_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5530420.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO179_000209"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO179_000209"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5530421.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO179_000210"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO179_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5530422.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OO179_000211"
     CDS             complement(36225..36941)
                     /locus_tag="OO179_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5530423.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO179_000212"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO179_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5530424.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO179_000213"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO179_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5530425.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO179_000214"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO179_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5530426.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO179_000215"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO179_000215"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5530427.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO179_000216"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO179_000216"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5530428.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO179_000217"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO179_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5530429.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO179_000218"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO179_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5530430.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO179_000219"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO179_000219"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5530431.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO179_000220"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO179_000220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5530432.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OO179_000221"
     CDS             complement(45790..46449)
                     /locus_tag="OO179_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5530433.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO179_000222"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO179_000222"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5530434.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OO179_000223"
     CDS             complement(47344..48357)
                     /locus_tag="OO179_000223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5530435.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO179_000224"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO179_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5530436.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OO179_000225"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OO179_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5530437.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OO179_000226"
     CDS             complement(50650..51828)
                     /locus_tag="OO179_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5530438.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO179_000227"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO179_000227"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5530439.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OO179_000228"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OO179_000228"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5530440.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OO179_000229"
     CDS             complement(55610..55888)
                     /locus_tag="OO179_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5530441.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO179_000230"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO179_000230"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5530442.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OO179_000231"
     CDS             complement(56470..57279)
                     /locus_tag="OO179_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5530443.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO179_000232"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO179_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5530444.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO179_000233"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO179_000233"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5530445.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO179_000234"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO179_000234"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5530446.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OO179_000235"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OO179_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5530447.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OO179_000236"
     CDS             complement(60980..61870)
                     /locus_tag="OO179_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5530448.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OO179_000237"
     CDS             complement(61857..62381)
                     /locus_tag="OO179_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5530449.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OO179_000238"
     CDS             complement(62378..62848)
                     /locus_tag="OO179_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5530450.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OO179_000239"
     CDS             complement(62845..63393)
                     /locus_tag="OO179_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5530451.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OO179_000240"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OO179_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OO179_000241"
     CDS             complement(64140..64862)
                     /locus_tag="OO179_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5530452.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="OO179_000242"
     CDS             complement(64922..66154)
                     /locus_tag="OO179_000242"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5530453.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="OO179_000243"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="OO179_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67426)
                     /locus_tag="OO179_000244"
     CDS             complement(66863..67426)
                     /locus_tag="OO179_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5530454.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OO179_000245"
     CDS             complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OO179_000245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5530455.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OO179_000246"
                     /pseudo
     CDS             complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OO179_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OO179_000247"
     CDS             complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OO179_000247"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5530456.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72432..72716)
                     /locus_tag="OO179_000248"
     CDS             complement(72432..72716)
                     /locus_tag="OO179_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5530457.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73088..74428
                     /gene="fadL"
                     /locus_tag="OO179_000249"
     CDS             73088..74428
                     /gene="fadL"
                     /locus_tag="OO179_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5530458.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75179..75853
                     /locus_tag="OO179_000250"
     CDS             75179..75853
                     /locus_tag="OO179_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530459.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OO179_000251"
     CDS             complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OO179_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5530460.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77084..78016
                     /locus_tag="OO179_000252"
     CDS             77084..78016
                     /locus_tag="OO179_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5530461.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78090..78164
                     /locus_tag="OO179_000253"
     tRNA            78090..78164
                     /locus_tag="OO179_000253"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78123..78125,aa:Arg,seq:cct)
     gene            78326..>78784
                     /gene="intS"
                     /locus_tag="OO179_000254"
     CDS             78326..>78784
                     /gene="intS"
                     /locus_tag="OO179_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5530462.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19966 a        21038 c        19326 g        18454 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgtgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc actctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt
    67501 atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt
    67561 cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa
    67621 agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct
    67681 taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac
    67741 agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccact tttgacaata
    67801 taatatataa acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat
    67861 tcaactctat tttctctatt atctgtatta ataaatgaca atgagtgtca aacagatatt
    67921 attcatttca acatgtaact ttgggaaaaa tcaagatacg cgcagaagaa taatttccga
    67981 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
    68041 cagatatccg ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt
    68101 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
    68161 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
    68221 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
    68281 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
    68341 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
    68401 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
    68461 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
    68521 agtggtcaga ggacgaaccg aatcactggc ggaatcgagg gctgcgtcgc cgtgacgcat
    68581 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
    68641 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
    68701 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
    68761 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
    68821 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
    68881 gggaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
    68941 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
    69001 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
    69061 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcac ccctgccctt
    69121 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
    69181 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
    69241 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
    69301 taattttagt cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa
    69361 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
    69421 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
    69481 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
    69541 gcgccgatgt acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga
    69601 agaaatgcag gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
    69661 gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg
    69721 ccaccatctg ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
    69781 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
    69841 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
    69901 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
    69961 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
    70021 atgccgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
    70081 tactacgcaa agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag
    70141 cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
    70201 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
    70261 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
    70321 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
    70381 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
    70441 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
    70501 cgggtaacaa tccaagttgt acttcagaca aaccgagcac cgttttagga tcgtcagtac
    70561 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
    70621 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
    70681 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc
    70741 cggcgataaa attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt
    70801 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca
    70861 ttttctcatc cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag
    70921 taaacgctga tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
    70981 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
    71041 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc
    71101 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
    71161 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc
    71221 ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag
    71281 cggtgttgac caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
    71341 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat
    71401 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
    71461 gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
    71521 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
    71581 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
    71641 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
    71701 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
    71761 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
    71821 cagcacgtgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
    71881 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
    71941 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
    72001 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
    72061 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
    72121 caccatcttc cctaaatcaa ccgtgggaat gccatgaaaa gccgtcgcct ggcgggcaaa
    72181 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa
    72241 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
    72301 tcttaaccat ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac
    72361 agtgaagaca gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    72421 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    72481 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
    72541 cacggttcgg attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct
    72601 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    72661 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    72721 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    72781 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    72841 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    72901 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
    72961 tagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    73021 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    73081 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    73141 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    73201 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    73261 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    73321 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    73381 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    73441 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
    73501 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    73561 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc
    73621 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    73681 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    73741 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    73801 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    73861 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    73921 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    73981 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    74041 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    74101 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    74161 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    74221 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    74281 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    74341 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    74401 cggtactaac tttaactacg cgttctgata acgcgttcgc ctagataaag tcacctgcta
    74461 tgcaggtgac tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg
    74521 agttttgcaa acagcctgat ggcagcttgc cctttcaaat cagtaagtag tatccacaga
    74581 caaacctgaa gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac
    74641 ttttctgcat ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt
    74701 aaagataggt tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata
    74761 tttgtaacaa atcaatcaac atggaataaa atcatgctac catctatttc aatcaacaat
    74821 accagcgcag cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta
    74881 gtacaagagt tcaaaaacca tttttaatgg taaggaagga ataagcacct gtattaaaca
    74941 tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata gatctaatat
    75001 taataattcc tcagttactt atattgatat tggctccaat gatacagacc atattactat
    75061 tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt
    75121 ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat
    75181 gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct tcgataagat
    75241 atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaaaa gcgaaccaac
    75301 ggacttattt acctggtacg gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa
    75361 aaataaaaac ggaaatgatt acttatttct cggttactac gatcaggacg actaccacat
    75421 tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg aaaacgccag
    75481 gagtgcttca gcgtggtttg aaagcagtaa agctatcatg gcagaatcat ttgcaactgg
    75541 ttccgatcat caggttgtaa acgagctcaa cggggaaaga ctgagagaac ctaacgaagt
    75601 ttttaaacgt ttaggtcgag caataagata taattttcaa gtggacgatg caaaatttag
    75661 acgcgataat gtaaaagaaa taatttctac tttattcgct aacaaagttg acgttgacca
    75721 tcctgaaaat aaatacaaag actttaagaa tctcgaagat aaagttgaaa aaagacttca
    75781 aaatcgccag acgaaatatc aaaatgaaat taatcaacta tctgcactag gtgttaattt
    75841 tgatgatatt taaaattatt tttccagttt tttgattttt caacgcctga atatcggaaa
    75901 tagtcagtga caaacatcag tatttttaca tcattacgtc cgggtcattg tcggtttatc
    75961 tccttttact tgtggtaatt gagggataac aacaaaaaag gtgagttttg cgactcacct
    76021 tttttatttg tttcttattc agaatcgata tcttttaaat catcctgaat cgcttgcgcg
    76081 ttcggatttt cctgcggttt gagttcgccg ccattagcga tgaaatcatg acgctggaag
    76141 tacgcttcgc gcaccataat ataaggatcg gacgactgac gcagcagacc atcggaatcc
    76201 agcagctgag cgcgggtttc gatcccttca agcgtccatt tacccacaga catcggccag
    76261 gtcagccagg aaagaaccgg gtaaagagca tccgccatat caccaccgtc atcacgcagc
    76321 gtgaagctac cgtagaacgg taactgaacg taaggcccat aacccacgcc ataatgacca
    76381 agcgtactac cgaagcggtg aggttcagtc cgttgcagtt tcgggttcgc catccctgca
    76441 acatcaataa agccgcccat ccccaaaatg gtgttcagga aaaagcgggt aaagtggacc
    76501 atcccctgat aagggtcgcc ctgcaagaag tagttaacca tcaccgcagg ttcttcaagg
    76561 ttgccagtaa agttgctcaa accattacgc gccggttgcg gaacataatc acgccaggcg
    76621 acagcgaccg gtcgaacaat atacgggtct aatacgttga agttgaagtt gtacatggtg
    76681 cggttgaacc cttctaacgg gtcagaacgc ccttgctgat ctgtaccgga actcgcacac
    76741 cccaccagaa gcgtagttcc cagagcaagc gccgacaggc gaagcttcat aaatgtctcc
    76801 ctgttttttt atggcttatg cagtttgcca tccatgacgg aacgataccg tatccgcctg
    76861 tttaggtgtg ggcgattgta acagcacgtc aactgatgtc cagacgccct gatttgctga
    76921 tttgatcata gcctggtaat cgccgccctg taggctactt gattctatag aaacagaaaa
    76981 aggcaaacgc agccttttct acattttcag agtaactccc gccagttgcg agcaaaaaag
    77041 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
    77101 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
    77161 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
    77221 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
    77281 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgccg aactggaagg
    77341 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
    77401 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
    77461 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
    77521 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
    77581 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
    77641 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
    77701 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
    77761 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
    77821 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
    77881 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
    77941 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
    78001 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
    78061 agtccattag cgggtatact cgccgcattg tcctcttagt taaatggata taacgagccc
    78121 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accatttatc agttcgctcc
    78181 catccgtacc agtccgcaaa atcccctgaa tatcaagcct tccgtatatt cacagttcgt
    78241 catggttcgc gtcagatcgt tgacagccgc actccatgac gggtaaaaag tggataaaat
    78301 aattttaccc accggatttt tacccatgct caccgttaag cagattgaag cagcaaagcc
    78361 gaaagaaaaa ccataccgcc ttctcgatgg taatggcctg tacctttatg tccctgtatc
    78421 cgggaaaaag gtatggcagc ttcgctacaa gattgacggt aaggagaaaa tcctgaccgt
    78481 cggaaaatat ccgcttatga ctttgcagga ggcaagggat aaagcatgga ctgcgaggaa
    78541 agacatctcg gctggcatcg atccggtaaa ggcgaaaaag gcttcgtcta acaacaattc
    78601 atttagtgcg atttacaagg aatggtacga acacaagaag caagtctggt cagtagggta
    78661 tgcgactgaa cttgcaaaaa tgtttgatga cgacatttta cccatcatcg gcggccttga
    78721 gattcaggat attgagccga tgcaactgct ggaagtaatc cgcagatttg aagatcgcgg
    78781 tgca
//
LOCUS       DAJTNW010000004        75780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNW010000004 DAJTNW010000000
VERSION     DAJTNW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(123..343)
                     /locus_tag="OO179_000255"
                     /pseudo
     CDS             complement(123..343)
                     /locus_tag="OO179_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(336..557)
                     /locus_tag="OO179_000256"
     CDS             complement(336..557)
                     /locus_tag="OO179_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530463.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(547..747)
                     /locus_tag="OO179_000257"
     CDS             complement(547..747)
                     /locus_tag="OO179_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5530464.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            877..1056
                     /locus_tag="OO179_000258"
     CDS             877..1056
                     /locus_tag="OO179_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530465.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1181..1510
                     /locus_tag="OO179_000259"
     CDS             1181..1510
                     /locus_tag="OO179_000259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530466.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1560..1748)
                     /locus_tag="OO179_000260"
     CDS             complement(1560..1748)
                     /locus_tag="OO179_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5530467.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2123..3351)
                     /locus_tag="OO179_000261"
                     /pseudo
     CDS             complement(2123..3351)
                     /locus_tag="OO179_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3600..4721
                     /gene="roxA"
                     /locus_tag="OO179_000262"
                     /gene_synonym="ycfD"
     CDS             3600..4721
                     /gene="roxA"
                     /locus_tag="OO179_000262"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5530468.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4770..5996)
                     /gene="pepT"
                     /locus_tag="OO179_000263"
     CDS             complement(4770..5996)
                     /gene="pepT"
                     /locus_tag="OO179_000263"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5530469.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6246..7382
                     /gene="potA"
                     /locus_tag="OO179_000264"
     CDS             6246..7382
                     /gene="potA"
                     /locus_tag="OO179_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5530470.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7366..8223
                     /gene="potB"
                     /locus_tag="OO179_000265"
     CDS             7366..8223
                     /gene="potB"
                     /locus_tag="OO179_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5530471.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8220..9014
                     /gene="potC"
                     /locus_tag="OO179_000266"
     CDS             8220..9014
                     /gene="potC"
                     /locus_tag="OO179_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5530472.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9011..10057
                     /gene="potD"
                     /locus_tag="OO179_000267"
     CDS             9011..10057
                     /gene="potD"
                     /locus_tag="OO179_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5530473.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10213..11034)
                     /gene="cobB"
                     /locus_tag="OO179_000268"
     CDS             complement(10213..11034)
                     /gene="cobB"
                     /locus_tag="OO179_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5530474.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11050..11961)
                     /gene="nagK"
                     /locus_tag="OO179_000269"
     CDS             complement(11050..11961)
                     /gene="nagK"
                     /locus_tag="OO179_000269"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5530475.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(11990..13234)
                     /gene="lolE"
                     /locus_tag="OO179_000270"
     CDS             complement(11990..13234)
                     /gene="lolE"
                     /locus_tag="OO179_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5530476.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13234..13935)
                     /gene="lolD"
                     /locus_tag="OO179_000271"
     CDS             complement(13234..13935)
                     /gene="lolD"
                     /locus_tag="OO179_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5530477.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(13928..15127)
                     /gene="lolC"
                     /locus_tag="OO179_000272"
     CDS             complement(13928..15127)
                     /gene="lolC"
                     /locus_tag="OO179_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5530478.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15389..16462
                     /locus_tag="OO179_000273"
     CDS             15389..16462
                     /locus_tag="OO179_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5530479.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16590..20036
                     /gene="mfd"
                     /locus_tag="OO179_000274"
     CDS             16590..20036
                     /gene="mfd"
                     /locus_tag="OO179_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5530480.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20179..21141
                     /gene="ldtC"
                     /locus_tag="OO179_000275"
     CDS             20179..21141
                     /gene="ldtC"
                     /locus_tag="OO179_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5530481.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21224..21436)
                     /locus_tag="OO179_000276"
     CDS             complement(21224..21436)
                     /locus_tag="OO179_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5530482.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21721..22353
                     /gene="comR"
                     /locus_tag="OO179_000277"
     CDS             21721..22353
                     /gene="comR"
                     /locus_tag="OO179_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5530483.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22415..22954)
                     /locus_tag="OO179_000278"
     CDS             complement(22415..22954)
                     /locus_tag="OO179_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5530484.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23181..24485)
                     /gene="ndh"
                     /locus_tag="OO179_000279"
     CDS             complement(23181..24485)
                     /gene="ndh"
                     /locus_tag="OO179_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5530485.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24893..25435)
                     /locus_tag="OO179_000280"
     CDS             complement(24893..25435)
                     /locus_tag="OO179_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5530486.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25458..26483)
                     /gene="nagZ"
                     /locus_tag="OO179_000281"
     CDS             complement(25458..26483)
                     /gene="nagZ"
                     /locus_tag="OO179_000281"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5530487.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26494..27318)
                     /gene="thiK"
                     /locus_tag="OO179_000282"
     CDS             complement(26494..27318)
                     /gene="thiK"
                     /locus_tag="OO179_000282"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5530488.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27299..27940)
                     /gene="lpoB"
                     /locus_tag="OO179_000283"
     CDS             complement(27299..27940)
                     /gene="lpoB"
                     /locus_tag="OO179_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5530489.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(27955..28332)
                     /locus_tag="OO179_000284"
     CDS             complement(27955..28332)
                     /locus_tag="OO179_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5530490.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28335..28694)
                     /gene="hinT"
                     /locus_tag="OO179_000285"
     CDS             complement(28335..28694)
                     /gene="hinT"
                     /locus_tag="OO179_000285"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5530491.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29033..31218
                     /gene="fhuE"
                     /locus_tag="OO179_000286"
                     /pseudo
     CDS             29033..31218
                     /gene="fhuE"
                     /locus_tag="OO179_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31278..32711)
                     /gene="ptsG"
                     /locus_tag="OO179_000287"
     CDS             complement(31278..32711)
                     /gene="ptsG"
                     /locus_tag="OO179_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5530492.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33006..33803)
                     /locus_tag="OO179_000288"
     CDS             complement(33006..33803)
                     /locus_tag="OO179_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5530493.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33814..34818)
                     /gene="holB"
                     /locus_tag="OO179_000289"
     CDS             complement(33814..34818)
                     /gene="holB"
                     /locus_tag="OO179_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5530494.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34815..35456)
                     /locus_tag="OO179_000290"
     CDS             complement(34815..35456)
                     /locus_tag="OO179_000290"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5530495.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35446..36468)
                     /gene="yceG"
                     /locus_tag="OO179_000291"
     CDS             complement(35446..36468)
                     /gene="yceG"
                     /locus_tag="OO179_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5530496.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36471..37280)
                     /gene="pabC"
                     /locus_tag="OO179_000292"
     CDS             complement(36471..37280)
                     /gene="pabC"
                     /locus_tag="OO179_000292"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5530497.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37400..38641)
                     /gene="fabF"
                     /locus_tag="OO179_000293"
     CDS             complement(37400..38641)
                     /gene="fabF"
                     /locus_tag="OO179_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5530498.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38728..38964)
                     /gene="acpP"
                     /locus_tag="OO179_000294"
     CDS             complement(38728..38964)
                     /gene="acpP"
                     /locus_tag="OO179_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5530499.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39175..39909)
                     /gene="fabG"
                     /locus_tag="OO179_000295"
     CDS             complement(39175..39909)
                     /gene="fabG"
                     /locus_tag="OO179_000295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5530500.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(39922..40851)
                     /gene="fabD"
                     /locus_tag="OO179_000296"
     CDS             complement(39922..40851)
                     /gene="fabD"
                     /locus_tag="OO179_000296"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5530501.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40867..41820)
                     /gene="fabH"
                     /locus_tag="OO179_000297"
     CDS             complement(40867..41820)
                     /gene="fabH"
                     /locus_tag="OO179_000297"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5530502.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41888..42958)
                     /gene="plsX"
                     /locus_tag="OO179_000298"
     CDS             complement(41888..42958)
                     /gene="plsX"
                     /locus_tag="OO179_000298"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5530503.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43039..43212)
                     /gene="rpmF"
                     /locus_tag="OO179_000299"
     CDS             complement(43039..43212)
                     /gene="rpmF"
                     /locus_tag="OO179_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5530504.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43264..43785)
                     /gene="yceD"
                     /locus_tag="OO179_000300"
     CDS             complement(43264..43785)
                     /gene="yceD"
                     /locus_tag="OO179_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5530505.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            43984..44568
                     /gene="yceF"
                     /locus_tag="OO179_000301"
     CDS             43984..44568
                     /gene="yceF"
                     /locus_tag="OO179_000301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5530506.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44680..45639)
                     /gene="rluC"
                     /locus_tag="OO179_000302"
     CDS             complement(44680..45639)
                     /gene="rluC"
                     /locus_tag="OO179_000302"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5530507.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46212..49397
                     /gene="rne"
                     /locus_tag="OO179_000303"
     CDS             46212..49397
                     /gene="rne"
                     /locus_tag="OO179_000303"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5530508.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49593..50546)
                     /gene="flgL"
                     /locus_tag="OO179_000304"
     CDS             complement(49593..50546)
                     /gene="flgL"
                     /locus_tag="OO179_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5530509.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50558..52200)
                     /gene="flgK"
                     /locus_tag="OO179_000305"
                     /pseudo
     CDS             complement(50558..52200)
                     /gene="flgK"
                     /locus_tag="OO179_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52266..53207)
                     /gene="flgJ"
                     /locus_tag="OO179_000306"
     CDS             complement(52266..53207)
                     /gene="flgJ"
                     /locus_tag="OO179_000306"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5530510.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53207..54304)
                     /gene="flgI"
                     /locus_tag="OO179_000307"
     CDS             complement(53207..54304)
                     /gene="flgI"
                     /locus_tag="OO179_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5530511.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54316..55014)
                     /gene="flgH"
                     /locus_tag="OO179_000308"
     CDS             complement(54316..55014)
                     /gene="flgH"
                     /locus_tag="OO179_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5530512.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55067..55849)
                     /gene="flgG"
                     /locus_tag="OO179_000309"
     CDS             complement(55067..55849)
                     /gene="flgG"
                     /locus_tag="OO179_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5530513.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(55987..56738)
                     /gene="flgF"
                     /locus_tag="OO179_000310"
                     /pseudo
     CDS             complement(55987..56738)
                     /gene="flgF"
                     /locus_tag="OO179_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56758..57966)
                     /gene="flgE"
                     /locus_tag="OO179_000311"
     CDS             complement(56758..57966)
                     /gene="flgE"
                     /locus_tag="OO179_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5530514.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(57991..58686)
                     /gene="flgD"
                     /locus_tag="OO179_000312"
     CDS             complement(57991..58686)
                     /gene="flgD"
                     /locus_tag="OO179_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5530515.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58698..59101)
                     /gene="flgC"
                     /locus_tag="OO179_000313"
                     /pseudo
     CDS             complement(58698..59101)
                     /gene="flgC"
                     /locus_tag="OO179_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59105..59521)
                     /gene="flgB"
                     /locus_tag="OO179_000314"
     CDS             complement(59105..59521)
                     /gene="flgB"
                     /locus_tag="OO179_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5530516.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59676..60335
                     /gene="flgA"
                     /locus_tag="OO179_000315"
     CDS             59676..60335
                     /gene="flgA"
                     /locus_tag="OO179_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5530517.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60411..60704
                     /gene="flgM"
                     /locus_tag="OO179_000316"
     CDS             60411..60704
                     /gene="flgM"
                     /locus_tag="OO179_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5530518.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60709..61125
                     /gene="flgN"
                     /locus_tag="OO179_000317"
     CDS             60709..61125
                     /gene="flgN"
                     /locus_tag="OO179_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5530519.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61165..62700)
                     /gene="murJ"
                     /locus_tag="OO179_000318"
     CDS             complement(61165..62700)
                     /gene="murJ"
                     /locus_tag="OO179_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5530520.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62867..63733)
                     /locus_tag="OO179_000319"
     CDS             complement(62867..63733)
                     /locus_tag="OO179_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5530521.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63735..64382)
                     /locus_tag="OO179_000320"
                     /pseudo
     CDS             complement(63735..64382)
                     /locus_tag="OO179_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64393..64977)
                     /gene="rimJ"
                     /locus_tag="OO179_000321"
     CDS             complement(64393..64977)
                     /gene="rimJ"
                     /locus_tag="OO179_000321"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5530522.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65213..66421
                     /gene="mdtH"
                     /locus_tag="OO179_000322"
     CDS             65213..66421
                     /gene="mdtH"
                     /locus_tag="OO179_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5530523.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66485..67132
                     /gene="grxB"
                     /locus_tag="OO179_000323"
     CDS             66485..67132
                     /gene="grxB"
                     /locus_tag="OO179_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5530524.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67266..67826
                     /locus_tag="OO179_000324"
     CDS             67266..67826
                     /locus_tag="OO179_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5530525.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            67932..68978
                     /gene="pyrC"
                     /locus_tag="OO179_000325"
     CDS             67932..68978
                     /gene="pyrC"
                     /locus_tag="OO179_000325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5530526.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69052..69297
                     /gene="dinI"
                     /locus_tag="OO179_000326"
     CDS             69052..69297
                     /gene="dinI"
                     /locus_tag="OO179_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5530527.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69587..69841
                     /gene="bssS"
                     /locus_tag="OO179_000327"
     CDS             69587..69841
                     /gene="bssS"
                     /locus_tag="OO179_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5530528.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            69956..71074
                     /gene="solA"
                     /locus_tag="OO179_000328"
     CDS             69956..71074
                     /gene="solA"
                     /locus_tag="OO179_000328"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5530529.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71122..71235
                     /locus_tag="OO179_000329"
     CDS             71122..71235
                     /locus_tag="OO179_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5530530.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71496..72062
                     /locus_tag="OO179_000330"
     CDS             71496..72062
                     /locus_tag="OO179_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5530531.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72066..72641
                     /locus_tag="OO179_000331"
     CDS             72066..72641
                     /locus_tag="OO179_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5530532.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72683..73735)
                     /locus_tag="OO179_000332"
     CDS             complement(72683..73735)
                     /locus_tag="OO179_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5530533.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            73960..74880
                     /gene="lpxL"
                     /locus_tag="OO179_000333"
     CDS             73960..74880
                     /gene="lpxL"
                     /locus_tag="OO179_000333"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5530534.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75052..75249
                     /locus_tag="OO179_000334"
     CDS             75052..75249
                     /locus_tag="OO179_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5530535.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75273..>75780
                     /locus_tag="OO179_000335"
     CDS             75273..>75780
                     /locus_tag="OO179_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5530536.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKV"
BASE COUNT        18473 a        20052 c        18749 g        18506 t
ORIGIN      
        1 gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat gcgcaatatt tccagcatgt
       61 tttcactgtg agaaagtgca agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca
      121 tattaatccc cgtccgtcgt ttttcttaaa ataacctctg tcacgaaatc agcataatcg
      181 gcggcgatat tcagtacatc aagcccgttg atttatattc tctcgtggaa agtaagaaaa
      241 aagccgctct aataagttct ggcattgacg aaagcgcatc agccgcatca tcaggaacgc
      301 cggaaaattc ctgtttcagg gaattaaaac gatcatcacg cataaccccc cccattttca
      361 caatcggcaa caagaatatt tttagcgtca ttcagtgacc gcgttgcgat gtatcggata
      421 cattccaggg cgaactgtgt ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg
      481 cattcaatat caataagcgc gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt
      541 gtggttttat tgtgcatagg tttccccctg acgaatacgg gcggcgaata cagcaacaca
      601 accggacggg caacggctac gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg
      661 ggattctccg gcactcagtg ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag
      721 gtgtggtata tgatttacgg caaccataac ggctcctagt ttacgttgtt ggttagaagc
      781 cccgttactg ctcctacagt gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc
      841 actacacata aatacattac ctgaggtgta attaacgtgt caacacacaa aaacgaaagg
      901 cgtggtaatc ctccgttcca gttccgttta gatcctgact tacgggaaaa gatggaaaag
      961 gcacaacaac aggacggcga tgattctctt gctgcctgga taaagcgaat aatccgcaaa
     1021 gaattacagc aacgcggcat agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag
     1081 gtactacggc gcattgcttt acaggcgcac acaatgacca acgaagaatc aaccgaaaaa
     1141 acatcacctg cacgaaaaag acgacgcaga aagaaagagc atgaatcaga acgattcgcg
     1201 ccgtgttctt ttgcccttga gaagtacctt aaagaatact catcaaagga gagaacttcg
     1261 cagttatggc aacgcactga accagactga ttgcattgcc caccagcctg atagcggcta
     1321 tcatccccgt gcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca
     1381 gaaaggcggc tccatttcgg ggccgctttt tttatgcctg aaaaacccca attttgtggt
     1441 tttccagttt caccagggcg aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc
     1501 atggttatag ctctgtgttc agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat
     1561 taatgaactg ccttgcagct atccttccag gccagaacct cggataaaga ccagccaacg
     1621 gaacgaccgc caagtttacg acgtgatggg aattgtccgg ccttttccag gcggtagcgg
     1681 catgagcggc taaggcctgt tagcttttcg cattcttttt cacgtataaa ccgatcagtg
     1741 cttaacacta ttgccccctt tcgtttctta aagagttatt tcgtgttcta ttgcgttggg
     1801 atgtgtctga ttgtgtcagg atgattcaga gttggcaaat gttgaggccg catggtttac
     1861 agaaagagga ataatcagga taaaatcctt taaattcatg ttaatacaaa ggcataaaat
     1921 attgtttcat gccttttttc tcgctattta aagagtgatt cgctagtgta taaaaaatca
     1981 gcaacacatt aaaaatcagc tatttataaa tctgtacact ttttcacctc ttgttcgtag
     2041 ttgttccgct ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc agtatgcgca
     2101 tactgaaaaa acacgaaaaa aattattttc ttctggctac tggtagcgtg gttacgtttt
     2161 catgtgttcc cgccagtatc cctaaccgct ccgtccacat atccagcgca ttgcgtttag
     2221 catccagata acgggaatga ttataaactc gctgcattcc tggcatctgg tggcctgtaa
     2281 gctgctccac gacgtgagga tccacgccta aatcgttcag catcgttgta aaggtgcgcc
     2341 ggatgtcatg tagtgaccag tgagggtgat ttagcctcct gtgcgctaat ctgccgtact
     2401 gcgacacgct tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc ctgttctgct
     2461 ccaccagctg cgtgacgaac ggcagtattg cttccggtat gggccggaat attgccacct
     2521 tcgttttgct gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc cactcgctga
     2581 tctccgataa cctcagttct accgtccggc atccgaacac aatcaggagg cggattaacg
     2641 cgatgtagta gggggagaat atttttttgt ccagtgcctg caataattcg cccagttctt
     2701 tggtgcttaa gacacgctcg cttatatccg gttttttccc aacgtccgcc acactcatat
     2761 catcaagaac gttgctgatt gcatagcgcc gcctccggca gaacttaagc gcctgtttgc
     2821 acgtctgtag caagaatccg gcagtaacag gcgttcgctt tgccacctgg tcaaaacagg
     2881 ccagccagtg ccgtagctcg catttatcca gcggcatagc accaatgtgc tgtattacgt
     2941 gattattaag gcgctttttc agggcggcat aatccacgcg gttttccttt gcgtacgact
     3001 caagccagta ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc tgtacggtgt
     3061 aattaagctc atgacgtgga ttttcccctc tgccagccat gcgcgacact gggcggcttt
     3121 ttccctggct gatttcaggt tcagatcagg ataatttccc agcttaatgc gttccggtgg
     3181 tgcccccctt cccgttccgg ccctgtaagt gaaataccag gttaaaaggc cactggttga
     3241 atgcctgacg ctcaggtttc caccgtcatt aagaaaggct gttttttggg cgggtgtgcc
     3301 gttgattttc ctcagctgtg tatcgctcag tttgttaagt gctctgctca taattttgat
     3361 tccggtcatc acaattaact acaccattaa ctacaccgat cgttgcacaa agggctacaa
     3421 cgtgaaacaa gctggaacaa ggaaaatcac aaatctgttt ataatcaaaa acataatgaa
     3481 caatctgaaa cattatgaaa cggcaaaaaa cactaattga taaaatatgt ccatacttca
     3541 cgcattacgt taagcatccg ttataatcgg ttgcagatac cagcctgtgg atgcttaaca
     3601 tggaatacca actcactctt aactggcccg attttcttga acgtcactgg cagaaacgcc
     3661 cggtggtgtt aaaacgcggc tttaataatt ttattgaccc gatctctcca gacgagttgg
     3721 cgggtctggc gatggaaagc gaagttgaca gtcgactggt cagtcaccag gatggcaaat
     3781 ggcaggtcag ccacggcccg ttcgaaagct acgatcatct cggtgaaacc aactggtcat
     3841 tactggtaca ggcagtaaac cactggcatg agccgaccgc cgcgctgatg cgaccgttcc
     3901 gtgaactacc ggactggcgt attgatgatc tgatgatttc tttttctgta cctggcggcg
     3961 gcgtcggccc gcatctcgat cagtacgacg tgtttatcat tcagggtacc ggacgtcgtc
     4021 gctggcgagt gggcgaaaag ctgcaaatga aacagcactg cccacatccg gatctgttac
     4081 aggtcgatcc gttcgaagcc atcatcgatg aagagctgga gcctggtgat attctttata
     4141 ttccgccagg attcccgcat gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg
     4201 gttttcgcgc gccaaatacg cgggaattaa ttagcggatt tgccgattat gtgctgcaac
     4261 gtgaactggg cggcaactac tacagcgatc cggatgttcc acctcgtgct catcctgcgg
     4321 atgttctgcc gcaagagatg gataaactgc gtgagatgat gctcgaattg atcaaccagc
     4381 cggaacactt taagcaatgg tttggcgagt ttatatccca gtcacgtcat gaactggata
     4441 tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta cgatgcgctg aaacaaggtg
     4501 atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca
     4561 atggtgagaa gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc agcaacattg
     4621 cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc gcgatgctcg
     4681 cggcgctggt caatagcggg tattggttct tcgaagggta agtttggatt taagccggat
     4741 gcagcatcac atgccgcatc ccttttcgct tacttccgtt gcgccgttaa ctcggcaata
     4801 cggacgatca cctgcaccgc tttttccata ccttccagaa tcacaaactc atgcttacca
     4861 tgatagttgt aaccgccagt gaacaggttc gggcaaggta atcccataaa cgacaactgc
     4921 gcgccgtcgg taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc
     4981 gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag
     5041 taactgtctt caatcaccaa ttcaatgtag caatcaggat gtaacccttt gcccactttt
     5101 ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg
     5161 atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa
     5221 ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt
     5281 gccgccagcg acagcgcatt taccatcact cctttcgccg tgcccggatg aacattgtta
     5341 ccgacaattt ttatattgac cgatgcggcg ttgaagtttt caaactccag ttcgcctacg
     5401 ccaccaccgt caacagtgta agcccagcgg gcatcgaagg catcaacatc aaaatgtttc
     5461 gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg
     5521 tttttctgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatca
     5581 gcacctaaca aggttttacc atcggtggta atcaacgtct gacccagtag ctgatgcagc
     5641 accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg
     5701 cgatagtttt caacaatttg cggattaaca tttttgccgc tgcaatccgg tgaggtatcc
     5761 acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc
     5821 atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatttcttc gagctgctct
     5881 ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgct
     5941 tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag
     6001 tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag
     6061 gtcactttta cccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct
     6121 aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc
     6181 tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg
     6241 tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc
     6301 aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga
     6361 ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg
     6421 ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg ctggataacg
     6481 aggacatcac ccacgttccg gcggaaaacc gctatgtgaa cactgttttc caaagctatg
     6541 cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa
     6601 cccccgctgc tgaaattacg ccccgcgtga tggaagccct gcggatggtg cagctggaaa
     6661 ccttcgctca acgtaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg
     6721 ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg
     6781 attacaaact gcgtaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggca
     6841 ttacattcgt tttcgtgact cacgaccagg aagaagcact caccatgtcg gacaggattg
     6901 tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc tacgaagagc
     6961 cgaaaaacct gtttgttgcc gggttcattg gcgaaattaa tatgtttaac gccacggtga
     7021 tcgagcgact cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct
     7081 acgttaactt cgccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact
     7141 tacgtgttga agagattaac gacgacaacc acgctgaagg gctgattggt tacgttcgtg
     7201 agcgtaacta caaaggcatg acgctggagt cggttgttga actggaaaat ggcaagatgg
     7261 tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct ctcgaccaaa
     7321 aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt
     7381 aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg
     7441 cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa
     7501 atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta
     7561 ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctatcca
     7621 tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg
     7681 attgttccgt tctggaccaa ctcattgatt cgtatctacg ggctgaaaat tttcctcagc
     7741 accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgacac accaatccgt
     7801 atcatgttca cgcccagtgc ggtgattatc ggtctggttt acattctgct gccgtttatg
     7861 gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc
     7921 gatctcggtg ccagcaagtt acagactttt atccgcatca ttattccact gacgatgccg
     7981 gggattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc
     8041 gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt acagttcctc
     8101 aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc
     8161 ctgatgttgc tggtttactg gcgcgcttct cgtttgctga ataagaaggt ggaactcgaa
     8221 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
     8281 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
     8341 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
     8401 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
     8461 cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc gttcgttagc ggaatgctgt
     8521 ttgtggtaat gatgtcacca gatatcgtga tggcaatttc gctgctggtg ctgtttatgc
     8581 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
     8641 catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag
     8701 cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt ctgccactgg
     8761 caatgccagc ggtggcggcg ggctgggtgt taagctttac cctgtcgatg gacgatgtgg
     8821 tagtttcttc attcgtcacc ggaccaagtt atgaaattct gccgttaaaa atttattcga
     8881 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
     8941 cgctggtgat ggttattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag
     9001 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
     9061 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
     9121 cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc
     9181 gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa acgtacaaag acggtgccta
     9241 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagagg ggatgatcca
     9301 gaagatcgac aagtcgaagt taacgaactt cagcaatctc gatccagaca tgctcaacaa
     9361 gccttttgac ccgaataacg actactccat tccgtatatc tggggtgcga cggcgattgg
     9421 tgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
     9481 agagtacaaa ggcagcctgc tgttgaccga cgatgcccgt gaagtgttcc agatggcgct
     9541 gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatataa
     9601 cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac tccgataacc cggctaaccc
     9661 gtacatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
     9721 ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa ggcggcatat tctggatgga
     9781 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt
     9841 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
     9901 ccttgcggcg cgtaagctgt taagtccaga agtggcaaac gataagacgc tctacccgga
     9961 tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt ggcgcagcca gcagcattta
    10021 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
    10081 accgcccgtt ttttagttag caaggatgtg gctaatgagt caggacagta aagttttatc
    10141 ccgctctttc gtcggtattg tggtccgctt gtaggcctga taagcgtagc gcatcaggca
    10201 atgcttcctg ctttataatc cctccagcaa cttttcaaca aattctggca ccacctggct
    10261 tgccgggccg taatatttct cggcaaattc attaccaacc tgactcggtt caagattcag
    10321 ttcaacggtg tgagcaccat gtagtttggc ttcgtgtaca aacccagccg ccggatagac
    10381 atgcccggaa gtaccaatgg caataaagac atccgccatc gacaacgcca tataaatttc
    10441 atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa
    10501 ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga gaacctgacc
    10561 actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat tggtattacc
    10621 tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat cgccgagagc
    10681 gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg gctgctgcag
    10741 ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc
    10801 cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt
    10861 acgaatacct gattccgcag aaattcctgc ccctgtcagt acgagtactc ttggtttttc
    10921 cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag
    10981 gcgacgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac
    11041 ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc
    11101 gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac aaggcaacct
    11161 gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag
    11221 gtcagggtca acaatggtca ggatatttcc cagagaaacc gctaataaat ccagataacg
    11281 ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa tttcgggagc
    11341 ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac cagacagata
    11401 attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat ccaaccccat
    11461 catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt
    11521 accagtaatc ggtttgccgt tgaaaaccag cccgccgcca acgccggtgc cgagaattaa
    11581 ccccatcacc agtggatatt gcgtaaattc atcatcccag gcttctgaaa gggcaaaaca
    11641 gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag
    11701 cggtttgccg ctggcagcgg ggacattagc ggcatacagc ttaccatctt ctgtttccgg
    11761 cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt cagcttcagc
    11821 taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg
    11881 cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc
    11941 aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc
    12001 taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa
    12061 gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg
    12121 gcaggaagtc aataaaataa atatcgctgg agaggaactg atgaccgatc agcttttcaa
    12181 tccactcaat aatcggggta agttgcagtg aaacaactac gccgataacc accccacaca
    12241 ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat
    12301 ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc accgccatca
    12361 ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca
    12421 taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca atccaacttt
    12481 taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta ttggcgttga
    12541 aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt
    12601 gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa
    12661 taccggcaat gtgcaaacgc acacgttttg gctgcatcag tttatgctcg ggattcgagt
    12721 tggggatcat gatcgacacc caatcgccct gcttcacttt cagcgcatcc gccacgcctt
    12781 tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcc ccctgaacaa
    12841 acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt aacgcccttc acctggattg
    12901 cgcgcagatt cgctccactt tccaccagac cggtgaaatt gatatacggc gcagcggcgg
    12961 caatacccgg cactttctgc acgttatcca gtgcttcctg ccagttagtc catggctgat
    13021 ttaccgcttc gatttcgcca tgcggcacca ccgccagaat gcggttattc agttcgcgtt
    13081 caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg gcaatgccaa
    13141 tggtagaaat gacagagatt agcgacacca tgccgccgcg tcgccgtccg cgactaaaac
    13201 gcaggccaat caataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc
    13261 cgccgtcaga cgtccatcac gcatttccag ttggcggctc atacgtttcg ccagttgcag
    13321 gtcgtgagtg accaccagga aagcggtgcc ctgcaagcga ttcaactccc caagcaactg
    13381 gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg ccagtaccag
    13441 acgcggatta ttgaccagcg cacgggcaat cgctacacgc tggcgttcgc cgccagaaag
    13501 ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca tctccagtgc
    13561 acagctgttg atttcagcgg gctttttctt gccaatcagc agtggcatag ccacgttttc
    13621 cagggcagta aaatccggca gcaggtggtg aaactgataa ataaagccca gcttctggtt
    13681 gcgcagttct gctttcgccg ctgaagacag tttgctcatc ggctgaccgt taaagatcac
    13741 atcgccggag gttggcgtat ccagcccgcc taacaggtgc agcaaggtac ttttaccgga
    13801 accagagcta ccgacaattg ccatcatttc accttcgccc acgctgaaac tgacattgag
    13861 cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt cgcattgcaa
    13921 caggatctta ttcataacgt aaagcctcag cgggttgtgt ggcggcagcg cgccatgaag
    13981 ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca atgacctgta
    14041 aaggttcgat agccaccggc agcgccgcgc catcaagcag gacgccgatt atcggcatca
    14101 gattatttaa ctggctggca agcagtgcgc caagcgccgc tccgaggatt gcaccgataa
    14161 tcccggcgct agccccttgc accataaaga ccatcatgat ttgtcgcgga gttaagcctt
    14221 gcgtttgcag gatcgcgact tcgccctgct tctccatcac catcagcccc agcgaggtaa
    14281 taatgttaaa cgccgcaaca gcgacaatca ggctcagcag taagcccatc atattttttt
    14341 ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc catttgctgc
    14401 cttcaggcag tttttgctga cttaaagagt caactctcag cggctcatcc agccacaaac
    14461 gccagccggt aatattggct gccggataac gcatcagacg cgatgcatcc tcaatattca
    14521 ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc acattgaaca
    14581 ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggc accatcacgc
    14641 ggatttgatc accgcgattg acgcccaact gtgaggcaag ttgttcgccg aggatgacat
    14701 tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc gttagtggat
    14761 ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca
    14821 gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt ttgactgccg
    14881 tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt gcctgtggca
    14941 tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact gacaatactg
    15001 tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag acgaaacgac
    15061 cgaagcgatc cactgcacgc ccacgcatgt aacgcaggcc aataaataga gcgacaggtt
    15121 ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc gaaagcaaga
    15181 tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc cccgtaaatc
    15241 aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa tctgaaaaat
    15301 catccaatac agacagatac ccattacacc tcgggtcata accagcagga tcaaccgcat
    15361 gtaaattaaa aaagagcgtg ttgttctgat gaaacaaaaa gagctatgga ttaaccagat
    15421 caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct tttatccgca
    15481 tatgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga tctatttcaa
    15541 tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct atttgattcg
    15601 ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct ggaacatctt
    15661 ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc tgaaccagtg
    15721 gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg attccaccgg
    15781 tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt atgcgttaat
    15841 tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat tcgccttgtt
    15901 tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt
    15961 gatacgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacaa taatgacctg
    16021 tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta ccgttctggc
    16081 ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat
    16141 catgaagctg ttttatcagt acgagcaacg attcggtatg cgctccacca gcctgctgaa
    16201 tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg ttgaaatatt
    16261 cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac
    16321 actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg gcttgctggt
    16381 aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt cactgcccgc
    16441 tgccgtcagt tactctcgtt aaaccatcac cccagccggg gcgattcaat ttgctaaacc
    16501 atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca tatgttgagg
    16561 catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat acgctgcccg
    16621 tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt gcaacgctgg
    16681 tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca gatatgcaaa
    16741 atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg atgaatctgg
    16801 cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt atctcctcgc
    16861 gcctttccac cctttaccag ctaccgacga tgcagcgtgg catactgatt gttccggtga
    16921 atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg ctggtgatga
    16981 aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc gccggttatc
    17041 gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg ttgctggatc
    17101 tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat gaaatcgaca
    17161 gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa gcgatcaatc
    17221 tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc cgcagccagt
    17281 ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag
    17341 gcacattacc tgctgggatc gagtactggc agccattgtt cttcagcgaa ccactgccgc
    17401 cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat ctggaaacca
    17461 gtgccgaacg tttccaagct gacacgctgg cgcgttttga gaatcgcggc gtcgatccga
    17521 tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc ttctcagagc
    17581 tgaaaaactg gccccgagtg cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg
    17641 ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcgcaacaa aaagcgccgc
    17701 tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa
    17761 gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac
    17821 gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg
    17881 aacatggttt tgtcgataag gcgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg
    17941 gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc gatacactga
    18001 tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag cacggcgtcg
    18061 gtcgctatgc cggaatgacc acgctggaag ctggcggcat tactggcgag tacctgatgc
    18121 tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat ctgattagcc
    18181 gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc gatgcgtggt
    18241 cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata
    18301 tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt gagcagtatc
    18361 agttattctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg
    18421 cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc ggcgatgttg
    18481 gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc
    18541 aggtagcggt gttggtgcct accacccttc ttgcgcagca gcattacgac aacttccgcg
    18601 accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag
    18661 agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg atcggtacgc
    18721 acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt gtcgatgaag
    18781 aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg aacgtggata
    18841 ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg agcggaatgc
    18901 gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc
    18961 gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg cgcggaggac
    19021 aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa cggctggcag
    19081 aactggtgcc tgaagcacgg attgccatcg gtcacgggca gatgcgcgag cgcgaactgg
    19141 aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt accaccatta
    19201 tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgt gcggatcact
    19261 tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat catcaggcat
    19321 atgcatggct gctgacgccg catccaaaag cgatgactac cgatgcacaa aaacgtcttg
    19381 aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg cacgatctgg
    19441 agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggttcaatg gaaaccatcg
    19501 gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg
    19561 agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg atgccgtcgc
    19621 tattgccaga tgatttcatc cctgacgtga acacgcgtct gtcgttctac aaacgtattg
    19681 ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc gatcgcttcg
    19741 gcctgctgcc agatccggcg cgtaccctgc tggatattgc ccgtctgcgc cagcaagcgc
    19801 agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc gaatttgccg
    19861 agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag ccgcagcatt
    19921 accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag cggaaaacgc
    19981 gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc gcttaatgta
    20041 aatcgggggc gtttctgcgc ccccatctgc aacatttaca aattctttgc actttcctgc
    20101 actatccgac actgtcacca tccataattc aggcttatct gtttattaca ataaccttat
    20161 atttattatg tatttttggt gatgatcaaa acgcgttttt ctcgctggct aacgtttttt
    20221 acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg gccgctgccg
    20281 ccagcgagca gtcgtctggt tggcgaaaac aaatttcatg tggtggaaaa tgacggtggt
    20341 tctctggaag ccatcgccaa aaaatataac gtcggctttc tcgctctgtt acaagctaac
    20401 cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt taacgatccc gttgcaaacc
    20461 ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca ttgcggagct gcgtctctat
    20521 tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa taggtattgg tcagttaggt
    20581 ggtgacacgc tgacaccgac aatggtgacc accgtttcag acaaacgtgc aaacccaacc
    20641 tggacgccaa cggcaaacat ccgcgcccgt tataaagcac agggaattga gttgcctgcg
    20701 gtagtgccgg ctggaccgga taacccaatg ggccatcatg cgattcgtct ggcggcctat
    20761 ggcggcgttt atttgcttca cggtacgaac gccgatttcg gcattggcat gcgggtaagt
    20821 tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca ggtcacccca
    20881 ggcaccaaag tgaatatcat caacactccg ataaaagtct ctgccgaacc aaacggtgcg
    20941 cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc gcagctgctg
    21001 ccaattacgc tgaatagcgc aatgcaatca tttaaagatg cagcacaaac tgacgctgaa
    21061 gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg ccgtcatcaa
    21121 gtgagcccac aaactctgta aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc
    21181 ggggaaaaaa tcagcagtag caggcattaa tgagggttaa tgcttattta taaattactg
    21241 ctgttccatg gagggtattc ggaccggtta cagaagtaat acggaaagat tttgcgccca
    21301 tctcatccgc tttttgcgcc agctgctctt ccagcgatcc cagatttgtc cccgcgttag
    21361 cactgatagt accgactttt tgttggcctt ctggcgttga ctgaacttcg acagccgcaa
    21421 agttggcaaa tgacatggag cttaaaatcg ccgcagcgat gaggttttta cgtttttcat
    21481 aatagtggcc ttatgcagat gaatgacgtc gcaagaaatt acttaacgat cgataactaa
    21541 atgatagatg tgatctggat cacatacaag atatttttta taacaatcat taattaattt
    21601 aaaaatcttt aaatttcagt caaatagaaa gcaattattt ttatgggatc tggactggtg
    21661 aacggaggtg attattttta taataacctt tcattcacag tcaccaggta caacggcatc
    21721 atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac
    21781 agggatgccg cgcttgataa ggccatgaaa ttgttctggc aacacggtta tgaagcgact
    21841 tctcttgcgg acctcgtcga agcaaccgga gccaaagcgc ccacgctata cgcggaattt
    21901 accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct
    21961 aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat
    22021 tttgcggcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg cttcatgatc
    22081 aacaactgca ccaccctctc cccagattca ggagatatag ccaatacgtt gaaatcacgc
    22141 catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc gcgcggggaa
    22201 atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat tattcagggg
    22261 atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat tgccggaacg
    22321 actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaggcccc tcagcctgag
    22381 cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc aaaatcagtt
    22441 gcccgttact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg attttgccct
    22501 gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac ttgtcataca
    22561 ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga gagccctgga
    22621 tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca tctttaccgc
    22681 gaccaccacc aaactgatgc cctatcacgc caccagcaac agcgccgagc accgacccgg
    22741 taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg cgacactcct
    22801 gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga gcgtattgcg
    22861 ggccccgttc aaacacgttc agactggcca ctgccgctac gcccaacgca gctgcgacac
    22921 caatcccgat acccgccaac attgatttat tcacgggaac acctccttct ttgcagttat
    22981 gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa
    23041 tgactggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc gacgcttaac
    23101 gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc gtttatacca
    23161 catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac gcggttaata
    23221 ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat ctgatgcatt
    23281 cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat tgagccgcgc
    23341 gtcaggttac ccatcaggct accaacggtg gagaagttcg acagcgatac cagcgaacca
    23401 tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag aatgttgttc
    23461 attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc
    23521 ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg agtggtttgc
    23581 agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc gatatctttc
    23641 aggaagtctg gcgctttgat cccggctgcc cataccatca gatcagcctc aatatattcg
    23701 ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg cgtcagcacg
    23761 cgaacgccaa gtttcgttag ctcgttgtgg gccgcagtag agatacgtgg cggtaatgca
    23821 ggcaaaatac gttctcccgc ttctaccagc gttacgttca gggcttcgtt ggtcaggcct
    23881 ttgtaaccgt agctgtgcag ttgcttgatc gcgttgtgca attcagcgga gagttctaca
    23941 cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc catttgcgcc caggttggca
    24001 gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg gtgcgggtta
    24061 tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga aatcgttaga ggtgctaccc
    24121 agcgccatga ccagggtgtc ataggcgatt ttacgttccg gaaccaacag ttcacctttc
    24181 tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac gatcaatatc aatgacggaa
    24241 cccaactgga actggaaacc atgattgcgc gcatgggcca gatagctcaa cgcatcgacg
    24301 ccttcatcaa gcgagccagt cgccacttcg tgcagcagtg gtttccacag gtggctgtgg
    24361 ttacgatcga ccagcgtaat tttggctttt ttcttacgtc ccagcttatg ccccagctgt
    24421 gttgccattt ccagcccacc agcaccgccg ccgacaatca caatcttttt caatggcgta
    24481 gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat taacatagct
    24541 cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa taacatgaat
    24601 ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgcttaaa agataggtaa
    24661 ctttaccggg ggagatcaaa aaatagagtt tattgtttat tatttgagca aattcggaag
    24721 ggtaatatag ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag atgcgcagca
    24781 tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg gacgccttat ccgacttaca
    24841 gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga
    24901 gggttttgaa cgctttaatg cgctgtaaat gcggggagat attcttgaat ttgtgcgtct
    24961 gctcttcgtc ccagacaatc tcgtaataat gatgcaactc ttcagatgtc cgctggctgt
    25021 taagtgcttc atcattacgc gacaaaataa ccagacaacg atcgcgattc ttctcgcgga
    25081 agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt
    25141 ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa
    25201 aaccaatccg ttccgcccag tatccgccca ggccaacgcc gcaaattagc ggacgctcgt
    25261 caacgttcag ttgcaacatt ttgtccactt ctttaagcag atgctgcata tcatgtttcg
    25321 gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga
    25381 ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt
    25441 cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga
    25501 ttcagacggg tgctgatcgc tttccagcga gccgagtcca tcagttcctg tcgcgaaaat
    25561 gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac
    25621 acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc cgcatccagt
    25681 gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat cgataaatcg
    25741 tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca aaacggtttt cagccagtag
    25801 ggagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc aggcataatg
    25861 gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt agcgcgaatc
    25921 tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt tactgcaccg
    25981 tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc atcaataaac
    26041 cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc gccaatcgcc
    26101 gcgctgatat gcccgacatc cagcactggc gcaaagctga tatcaatatc catagcgatc
    26161 atttcgctgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc ttccattccc
    26221 aacagcgcag caaaggattg tgctgccggt aagcgggtaa aaccttcgcg aaaacgctgc
    26281 acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgatgc tgcgcggatc
    26341 tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc
    26401 cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc gtaaccttcg
    26461 acatccaaca ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca
    26521 ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc ggtactcaaa
    26581 ccaccctcct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg
    26641 cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt gctgttcagt
    26701 attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccag catactccca
    26761 gtcgatgagt tttaaccctg atgcgctatg cactaaattt ccggcgtgga catccatatg
    26821 caatggactt aagcgtaaag gtcgtggttc ccgcgctttg cgcagacgtt ttaacattcg
    26881 cagccaaccc acagtccgcc gcgccggatc gctttgctgc cagtacagtt ccagtaaggg
    26941 caacagcgtt attcgccagc caaaacgtgg ttgttgatgt agataataca gcaagcctgc
    27001 cagttcgttg gtatccggca aatacgtttt taccgcgccg ggcagatagt cgactaccat
    27061 ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt gtgataaagc
    27121 ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct gacgcacaac
    27181 aaaacgctgg ccctgatgtt caatgagaaa actaccgcca ctaagcccac tattaaacgt
    27241 cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt
    27301 attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc accagcatca
    27361 gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac agcacgtaat
    27421 gagcgccgac attgcgggca atgcctatgg ctttactacg ggtgcctaaa ctgtcctgcg
    27481 gcgacaaacc taactgttgc ttagccatcg acagttgctg ggcggaaacc agggtaaatt
    27541 tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac
    27601 cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc accccgtcag
    27661 ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa tggcgaatat
    27721 gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg gcctggctgc tgcgggatcg
    27781 tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt tccggcgctg
    27841 gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg agaaacatcg
    27901 ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac ccctttacaa
    27961 atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac
    28021 cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca tctccagccc
    28081 tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc tttcgttata
    28141 aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct tttctgcact
    28201 gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat tcaccggaat
    28261 ttccggatgt gaacgacagc ccaccagcag taacaacacc agagacacca gcccaaagca
    28321 tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg acggccaccc
    28381 aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt attcatgatc
    28441 agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc tacggtgatc
    28501 atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg aatgagaata
    28561 ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc
    28621 agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt gctgaatata
    28681 gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc atgctacgcc
    28741 gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa tcttaactca
    28801 cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt cccatttctg
    28861 cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata tgaatgcgta
    28921 tatttctcat ttgcatttgc atttgcagca aaattattcg cacactaaat ataaccgctc
    28981 atcaccctcc tccggcatga gcctgacgac aacataaacc aagagatttc aaatgctttc
    29041 aacacaattt aacagggata atcaatatca agtcatcacc aaaccgtcac tacttgccgg
    29101 ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca ctgaagaaac
    29161 ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt acagcgtaac
    29221 gtctacctct gcgggtacca aaatgcagat gactcaacgt gatattcctc agtcggtcac
    29281 tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg aagtgatgga
    29341 aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt attattcccg
    29401 cggattccag atcgataact atatggttga tggtatcccc acctattttg aatcgcgctg
    29461 gaatctgggc gacgcacttt ctgatatggc actgtttgaa cgcgtagaag tagtgcgtgg
    29521 cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata tggttcgaaa
    29581 acacgcgacc tgtcgtgaat ttaaaggcga tgtctcggcg gaatacggta gctggaacaa
    29641 agaacggtat gtggcggatt tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg
    29701 aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaata gtgaaaagac
    29761 cttcttctcg ggcattgtcg atgctgattt aggcgatctt acgacgcttt cagccggtta
    29821 cgaatatcag cgcattgatg ttaatagccc aacctggggc ggtttaccgc gctggaatac
    29881 tgatggcagc agcaacagtt acgatcgcgc acgcagtacc gcacctgact gggcgtacaa
    29941 cgataaagag atcaacaagg tctttatgac cctgaagcag cggtttgctg atacctggca
    30001 agcgacactg aatgccaccc actctgaagt cgaatttgac agcaaaatga tgtatgtcga
    30061 tgcctatgta aacaaagcgg atggtatgct ggttgggcca tacagtaatt atggacctgg
    30121 ctttgatcat gtcggcggca ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga
    30181 tttgttcgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg
    30241 cagttacagc aaacaaaaca atcgttcagt tcatgggcca acatcttccc ggatgaaatt
    30301 ggcagtttct acaactttaa tggcaatttc ccacaagccg actggtcatc acagagcctg
    30361 gcgcaggacg ataccacaca tatgaaatcg ttatatgctg ccactcgtgt cacccttgcc
    30421 gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact
    30481 tacagcatgg agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac
    30541 aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt
    30601 tcaggcaaat atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac
    30661 tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc
    30721 gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg
    30781 gatgggacag tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg
    30841 cagctgacat ttggcgcaac gcgctatctt gcagaggata acgaaggaaa cgccgttaat
    30901 cctaatctgc cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca
    30961 gagttgacag tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca
    31021 ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc
    31081 taccaggtga cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc
    31141 tacgatacca acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc
    31201 ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggct gccttagtct
    31261 ccccaacgtc ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta
    31321 tcggatttag taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg
    31381 cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga
    31441 cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga
    31501 gccggtgcca tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc
    31561 ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca
    31621 taaccgatac cgacgatcgg taacagccac agtttgctgc tgttaccaga cagaacgatg
    31681 aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt
    31741 gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg
    31801 aagaagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc
    31861 ataataccgc ccactttcgc acggttttct ggtttagcag agtgccagat agcaattgcg
    31921 gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg
    31981 tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat
    32041 tcaccaatct gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggtaccagg
    32101 caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac
    32161 tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca
    32221 gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca
    32281 agatactcag gcagcttaat acggtagaaa cggttaaaca tgtacgctgc aatcgcaccg
    32341 gagataatcc ctccgagtac gccagtatcc gccaagtgtt tagaggcgat ttcttcagca
    32401 ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca
    32461 acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc
    32521 gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat
    32581 acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata
    32641 ggtagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca
    32701 ttcttaaaca taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt
    32761 ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca
    32821 gagtaaaata attcagtgcc aatttgtttg acggctatca cgtttcaact tcagatcatt
    32881 ggagaagttg cacattttga caaaacatat gtcacaatat ttcaaacgcg gttattcacc
    32941 gcccaatttt cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa
    33001 ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta
    33061 tcggtggtta cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct
    33121 gcaacgtcac gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg
    33181 taaggtgagt cagtttccac cagtaatcga tccaggggga cataacgcgc agcatcgcgc
    33241 agttgctccg cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc
    33301 agtaatttac ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc
    33361 gtcacttttt cttcgcgcag aattgccagc gtatcggcac gggcgtcacg ggtatggacg
    33421 ataaccggct tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga
    33481 cgtactttag tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca
    33541 ccctcttctg ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt
    33601 ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt
    33661 aaataacccg gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct
    33721 ttcgccagaa cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg
    33781 cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac
    33841 gcccggttgc aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt
    33901 gatgcctgtg acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg
    33961 caggcgcgag ggagaaagat ggtttgccag ttcggcgacc agtcccggca catcaacatt
    34021 ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca
    34081 gtgtaaacgc gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc
    34141 cgatggcacg ctatatgcca acgcctgaca caatgtttca cgagcctgcc agttatctcc
    34201 ctgaaacaac gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa
    34261 tgcatcctgt gacattgtca cttcgcgtga aagccaagtc acggcgtact gttctggagg
    34321 cggcgcaagg taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc
    34381 gcgggtagcc aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc
    34441 gttagccgcg gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc
    34501 taggcgtgcg tgctcattca gcttttcggt gacctcacgt accgcatcaa tgcccagcgt
    34561 atttttccct ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa
    34621 ctgacatcca cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata
    34681 acggctcagg gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag
    34741 cgcatggtga cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa
    34801 ccatggatac catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt
    34861 gcatccatca cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt
    34921 gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca
    34981 atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg
    35041 agatagagcg ttaagtctgg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc
    35101 atatgttggt caataccacg tcctccgccc tgatacgcct gagtggaaag atcgtggcga
    35161 tcgccaatca cccaggtgcc gttagccagc gcaggtttga tgaccgtttc taccagttga
    35221 acgcgcgcgg cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc
    35281 gatttgatat ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc
    35341 cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg tctcaaccac cacattacgc
    35401 gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt
    35461 ttccttaagc actttcagat aatcctgcac agacttgtta tgactggcaa gattggtatt
    35521 aaacgtgtga ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg
    35581 cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt
    35641 aatggtatag gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt
    35701 ataacgctct cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg
    35761 taaacggttg ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt
    35821 ttcgataatt gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc
    35881 acgcccttcc caggcgctat cgaccgcttt caccattttc ttgtgaactc gcttgagtaa
    35941 cgcgacatcg gtggtattgg cggtatacat caaggtgtct ggccagaacc aaccttcaat
    36001 ccactccggg ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag
    36061 cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc
    36121 ttctaccagt cgcagcggga actgtgcttc tttaccgctt tccaacaatt tcagcatctc
    36181 gcgcacggtc atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg
    36241 ttcgatacgc agcagccatt gaaaaacccg tgggcgattg atgatcttat cagcataaag
    36301 ctgttcaccg agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt
    36361 gataagcaat ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat
    36421 acccagtacc accagcaata acaagataat caataacact tttttcatga ctaattcggg
    36481 cgctcacaaa gtggggctaa atattcatat aacgttgctg acgaaaaggt gacatcgcca
    36541 caggcacgta cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc
    36601 aacgcctcct ccagcgaggc ttgcacttcg acaagctgat aagaggattg tgccagtaaa
    36661 cggatacaga attgtcgcat aatgccgtta acacctgcct gatccagtcg cggcgtataa
    36721 actacattgc ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg
    36781 ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg aatcaatact
    36841 tgctcaagcc gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc
    36901 gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataagcc
    36961 gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg tgctgtaccc tcgcccgcca
    37021 ctaccgcgac tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc
    37081 atctcttgtt caagctgagg ccagaagtca caggaaatca tcaaccgctg acaagcatcc
    37141 tgtagtcgca ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg
    37201 gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta
    37261 taaccgttaa ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt
    37321 tcactacgga acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat
    37381 atgggtggaa aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga
    37441 agccgaagga gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta
    37501 cgaaatccag atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct
    37561 gatcgcgcag cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt
    37621 gaccggtcat agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg
    37681 ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa
    37741 cgtagccaat ctgactcgct tcaatgcctg catcacgcag agcatttgcc attgccagag
    37801 ctgcgcctgc gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa
    37861 agccgacgag ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa
    37921 gtaccagcat accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc
    37981 ggctcgccgc ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac
    38041 caacgcccag cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc
    38101 catacgcgat aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg
    38161 cgatagagat gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt
    38221 tcacaatcgt tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag
    38281 atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa
    38341 tgcgggttgc gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag
    38401 cgacaattcc atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt
    38461 cctcacagtt aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga
    38521 aatggtcgat taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta
    38581 cggtattgcc gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca
    38641 cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac aaaaagataa aactcaggcg
    38701 gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt aatcaatggc
    38761 agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat caaactcttc
    38821 ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt cttcaacgaa
    38881 agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga taattttctt
    38941 aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg ctttcgcgat
    39001 ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca ggtcttacca
    39061 cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt gcactaatta
    39121 ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac
    39181 catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga
    39241 tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac
    39301 ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat
    39361 aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc gcgccagtga
    39421 tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc
    39481 catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt tcatcatagc
    39541 gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttagtttcga taatatcgtt
    39601 ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg cattattgac
    39661 caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt cgatagatgc
    39721 cgggtcggtc acattcaaca tcagaccttt gccgttggca cctaaataat cactgatcgc
    39781 ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt
    39841 ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga tttttccttc
    39901 aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg aaggttcgtt
    39961 cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa gcaccttgcc
    40021 cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa cagacttcgt
    40081 ccactgaacc gggttataca actgacgtac cagtgcgtca cggatggcat caccattggt
    40141 ttcgcatttc acatcaacgt tattcacaac agaaactgtt ggtgcgttaa aggtgatttt
    40201 cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt gagacggtac
    40261 gctcactggt aacggcagcg cgcgttttgc gccagcagct ttacaggcag cgccagcacg
    40321 ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa agtttaccgg
    40381 agaaacgacc tgaccttctg cagcttcttc acacgctttc gcaataggcg catcatccag
    40441 accgatgatt gccgccatag cgcccgtgcc ttccggtacg gcttcttgca tgaacttacc
    40501 gcgcatctca accagacgca ccgcatcagc gaaatcaatc acgccagcgc aaaccagcgc
    40561 ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc cctgctgctg
    40621 ccatacgcga tacagcgcaa cagatgcagt caacagcgct ggctgggttt gccaggtttt
    40681 attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc
    40741 agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat cagccagcat
    40801 tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca tgttttaatc
    40861 cttatcctag aaacgaacca gcgcagagcc ccaagtgaat ccaccgccaa aggcttcaag
    40921 cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca gcgcgcacgg
    40981 gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat tgtccatcga
    41041 cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat gcggaaccag
    41101 ccagtccagt tgagaacggt caagattatt cgccgccagc gtctcatcaa cgatgtgcgc
    41161 cagttccgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat gaattgaatt
    41221 ctctggattc acgcggtcgg cgtttggcag cgtcagcaat tcaccataac taccgtcggc
    41281 atgcagatgg gtggaaatga ttcccggctc ttcagaggca gccagcaccg cagcgcccgc
    41341 gccatcgcca aaaataataa tagtcccacg atctgttgga tcgcaggtgc gcgccagtac
    41401 atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt gatcggctac
    41461 gcttaatgca taggtgaaac ctgcacaggc tgctgcaacg tcaaatgccg ggcaaccttt
    41521 aatgtccaac atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt
    41581 cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct caattgcgcg
    41641 tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc
    41701 gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt tttccaaatc
    41761 ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct tcgtatacat
    41821 gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt tcgctggcag
    41881 cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa ccagctgggt
    41941 atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc gcctgcactg
    42001 cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt tttatcaccg
    42061 tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg tgactgaatc
    42121 gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac gaccgttttt
    42181 tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct tccatcgtct
    42241 ttaacgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg ccagttaaca
    42301 actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta agcaccgctg
    42361 aggcatcccg aatactgtca agacccttta cttcttcttc accaatattg agcaacgcca
    42421 cgcgaggatt gggaatttcc accacctctt cagccagaac tgagcccata atggcaaatt
    42481 gcaccaacat tgtgctgtca caatcgacgt tggcccctaa atcgaggacc accgttttgc
    42541 ccttttgctg atgtggtaat accgtcacca gcgccggacg ctcaatcccc tccaggggct
    42601 tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg acacaggctt
    42661 gcgctcgacc ttctttcacc agctccaggg ccatacgcat tgaactccca cgactggcgc
    42721 ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga ataatctgca
    42781 gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg tcgggattgc
    42841 cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct gcaggcactg
    42901 tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga cgtgtcaagg
    42961 tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct cactaagctt
    43021 catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc
    43081 agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca ggctggtgac
    43141 tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg gtttattctg
    43201 ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa cggggagcac
    43261 caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt gcacttcttc
    43321 aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag aatcatgcac
    43381 cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca gatcgatttc
    43441 accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag cctgttcgtc
    43501 tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg gcttcccgca
    43561 acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg cgagacgttg
    43621 gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga ctacggattc
    43681 ggcgacgcgc tcaacctgat caggggtata gataccctgg taatcaaggc gtttttgagc
    43741 cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc gcgcatatta
    43801 actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt tgccaataat
    43861 tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga acatcaacct
    43921 gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct gatggaaaaa
    43981 aatatgccta aacttatttt agcctccacc tcgccatggc gtcgcgctct tctggaaaaa
    44041 ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg aaaccccacg cagcgacgaa
    44101 tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct ggcgtcacgt
    44161 tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg cgaaatcacc
    44221 ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag cggcaatatc
    44281 gtcaccttct atactggact ggcgttgttt aattcggcga atgggcatct acaaacagaa
    44341 gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg cggagattga taattacgtg
    44401 cgtaaggagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt tggcattacg
    44461 ctgtttgagc gtttagaggg acgtgaccct aacacgctgg ttggtttacc gctaatcgcg
    44521 ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga tgggatagtt gtttgcgccg
    44581 gatgcggtta cgtcgcatcc ggcaatcggt gcagacggcc tgatgcggcg taaacgcctt
    44641 atcaggccaa ccgcccatta tcaggcttat atgcttgtat tagcgcgcgt tacgcagctt
    44701 ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg atacgcatca cctcaccggt
    44761 ccccggatgg gtaaacttca acgctgcggc gtgcaggaac agacgattta atcccgtgcc
    44821 tgcttcagtg agctgtctgt caaattcacg gtcaccgtag cgatcgtcaa aggcaatcgg
    44881 atgacccgca tactgtgtat gcacacggat ctgatgagta cgtccggtta ccgggctaca
    44941 acgcaccagg gtggcaaatg catagcgttc ttccacttta aagcgtgttt ccgacggttt
    45001 gccttcctga ctgacacgca cgatacgttc gccgctttgc agaatatttt tcaataacgg
    45061 cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca ggtaatcttt
    45121 ttgcattcct ttttcacgta attgctcatg cagagaacgc aacgccgagc gttttttcgc
    45181 taccagcaac acacctgaag tgtcccggtc gagacgatga accagttcaa ggaaccgcgc
    45241 ttccgggcgc aacgcccgca aaccttcgat aacgccgaag cttaaaccac tgccgccatg
    45301 taccgccgta ccggaaggtt tattcagcac caggatatga tcatcttcat ataagatgac
    45361 gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt cccgctcagc
    45421 aacgcgaacc ggtgggatac gcacctcatc acccgcttcg agtttatatt caggcttaat
    45481 acgttttttg ttcacccgca cttcaccttt acgcaaaata cggtaaatca tactttttgg
    45541 tacgcctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt cgtcagcggt
    45601 gatagcaaca atttttacgg atggagtctc tgttttcatg gtgggcgatt ctaattagcc
    45661 aacaggattc gcgccactca tttttctatg cttatattta gttttgcacc ttattacttc
    45721 actacgtgat cactttattg atggttatta aaccaatcac cagcaagaag tgaaaaaact
    45781 gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct tgcagtggaa
    45841 taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg aataacccat
    45901 tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt tcaggcagtt
    45961 agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag agtaataccc
    46021 agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa ccgacactct
    46081 gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca cgaggccatc
    46141 ggttcacacc cggaaaggcg ttactttgcc cgcagcttag tcgtcaatgt aagaataatg
    46201 agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag ttgcgcgttg
    46261 cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg cacgagcaga
    46321 aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg gaagctgctt
    46381 ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt gcccgcgaat
    46441 atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg ttgcgtgaag
    46501 gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc gcggcattaa
    46561 ccacctttat cagtctggcg ggtagctatc tggttctgat gccaaataac ccgcgcgcag
    46621 gtggcatttc tcgccgtatc gaaggcgatg accgtaccga attaaaagaa gcactggcaa
    46681 gccttgaact gccggaaggc atggggctta tcgtgcgcac cgctggcgta ggcaaatctg
    46741 ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca ctgggaagcc atcaaaaaag
    46801 ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta atcgttcgcg
    46861 ccttccgcga ttatttacgt caggatatcg gcgaaatcct tatcgataac ccgaaagtcc
    46921 tcgaactggc acgtcagcat atcgctgcat taggtcgccc ggatttcagc agcaaaatca
    46981 aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg cagatcgagt
    47041 ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc cattgttatc gacagcaccg
    47101 aagcgttaac agccatcgac atcaactccg cacgcgcgac ccgcggcggc gatatcgaag
    47161 aaactgcgtt taacaccaac ctcgaagccg ccgatgagat tgctcgtcag ctgcgcctgc
    47221 gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgccagta cgccaccagc
    47281 gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgtgcgcgt attcaaatca
    47341 gccatatttc tcgctttggc ctgctggaaa tgtcccgcca gcgcctgagc ccatcactgg
    47401 gtgaatccag ccatcacgtc tgcccgcgct gctccggtac cggtaccgtg cgtgacaacg
    47461 aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga agcgctgaaa gagaacaccc
    47521 aggaagttca cgccattgtt cctgtgccaa tcgcttctta tctgctgaat gaaaaacgtt
    47581 ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg ctgcgtgatt gtgccaaacg
    47641 atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg caaaggggaa gaaacgccga
    47701 ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg tctgaagaag
    47761 agttcgctga acgtaagcgt cctgaacaac ctgcactggc aacctttgcc atgccggatg
    47821 taccgccagc gccaacccca gctgaacctg ccgcgactgt cgtagcccca gcaccgaaag
    47881 ctgcaacggc aacaccagca gctcctgcac aacctgggct gttaagccgc ttcttcggcg
    47941 cactgaaagc gctgttcagc ggtggtgaag aagccaaacc gaccgagcaa ccaacaccga
    48001 aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa acctcgtcag agcaaccgcc
    48061 gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac tgaaggcagc gataatcgcg
    48121 aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac tgccgagacg cgtgagagcc
    48181 gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga cgagcagcaa gcgccgcgtc
    48241 gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa gcgaaggcgc
    48301 tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta cgtccggttc
    48361 agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa agcgtagccg
    48421 aagaagcggt agtcgcaccg gtggttgaag aaactgccgc tgccgaacca attgttcagg
    48481 aagctccagc tccacgcaca gaactggtga aagtcccgct gccagtcgta gcgcaaactg
    48541 caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc atgccgcgtc
    48601 gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca gcgtcgtcgt cgctatcgtg
    48661 acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcacg tctccggaat
    48721 tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat gtacaagttg
    48781 aagagcagcg cgaacaggaa gaagtacaag tgcagccgat ggtgactgag gtccctgtcg
    48841 ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga agaaatggct gaagtcgtag
    48901 aagcccccgt accggttgcc gaaccgcaac cggaagtggt tgaaacgacg catcctgaag
    48961 tgattgccgc cgcggtaact gaacagccgc aggtgattac cgagtctgat gttgccgtag
    49021 cccaggaagt cgcagaacac gcagaaccgg tggttgaacc gcaggaagag acggcagata
    49081 ttgaagaagt tgccgaaact gctgaggttg tggttgctga acctgaagtt gttgctcaac
    49141 ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga aacggtggcc gcggttgaac
    49201 cagagatcac cgttgagcat aaccacgcta ccgcgccaat gacgcgcgct ccggcaccgg
    49261 aatatgttcc ggaggcaccg cgtcacagtg actggcagcg ccctactttt gccttcgaag
    49321 gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc ctctgccgct cctgcgcgtc
    49381 cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc cctggtaact gccagggctt
    49441 ttttatttca tgtttgaatc aacatattaa aattcatcaa atggcatcct tgctaaccaa
    49501 caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc cagagcgggc agacaaaaca
    49561 tacccagttt catgatatgt tttaaagcga gcttatttgt tgagctggaa gagcgacaat
    49621 ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa
    49681 gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct
    49741 aaagcgcgat cgctacctaa ggaatccagc gactccagtt tgttcagctg cgtgcctaat
    49801 tccgcgcgaa cggtaaggac attattcagc gagtttttca gtccacggtt ggttttatcc
    49861 aatgccgcag cggcgatttc tttatccgct tcgctatccg cgaccggcgt tttcagggct
    49921 gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc
    49981 gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc
    50041 gaacgcgaag catcgacctg ttgcttaata ctttctgttc cgccaacata atcaccgtta
    50101 gcctcactaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg
    50161 tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc
    50221 gaggcccggt catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca
    50281 ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg
    50341 gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaaca
    50401 gcttgtgatg cagcaatggg atcgtctgaa gggttaacga ctcgcttacc cgtcgacatc
    50461 tgctcgccgt acttcatcca ttctgcctga gaattggtga tgccgcgcat gttttgctgg
    50521 tacatcatct gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat
    50581 caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac
    50641 gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg
    50701 aaagctgcgt caccacatta ccttgcgtgg tgctactggt tttcaacgtc gcggttttat
    50761 taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg
    50821 ttttactgtt gctttgcaga tccagcaggg cctgaccgtt gcggttgtcg ctatcacccg
    50881 catcttcttc gctcgccatc gcgattttcg cttcgtcggt gattaataca tccatgttga
    50941 cgatggcgtc acttactggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa
    51001 acgtcaactc cagaccatca aatgccactt taccgttggc atctggtgtc gccgtaaaag
    51061 tggtattgct ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat
    51121 ctgtcgccag taccacggag gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt
    51181 ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc
    51241 cgttagcatc aaatccggct ttgtgttggc tgttgaaagc ctcggcaaat gccagcgcca
    51301 gttgtccaag cgtattacgc gtctggtcca gatcctgaga acggaatgtc agaatgccgc
    51361 ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa
    51421 cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg aactgccgcc agttgccgcg
    51481 ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg atgttataag taccgccatc
    51541 ctgaacgctg acttctacgc caacaatctg gtttaattcg ctcaccagtt gatcgcgttg
    51601 atccagcaga ttgttaggtg acgcccctgc ccccacgcct gtcaggcgcg agatttgatc
    51661 gttcaggctg gcaatttgtt tagcgtaatt gttgatctga tcaacgctgg cacctatcgc
    51721 gatattgacc tgtttgtcct ggtcgcgcag atattgatcg gtggttttaa actgattcac
    51781 caacccttct gatttcccaa tcagcgcctg gcgcgccgcc gggtcttccg cgttactcac
    51841 cagcgtttgc aggctggtga agaaatcctg catctgtgtt gccagcgaag aggtactggt
    51901 ggagagcata ttgtcgattt tcgacatctg ctcatagcgg gcggtcaggc cgctactttg
    51961 cgtctgcgca gcacgtaact ggttggtaat aaacgtatca tactcacgct gcacaccaga
    52021 aacgtagacg ccattgccaa cccagccgcc agcgcccaac gtgctattgg cctgcgccat
    52081 aatagtggtt tggcgggtat atccggcaac gttatagctg gagatattat tacttgccgt
    52141 atttaacgcc gcctgggccg cgttcagtcc gctcatggcg ttattgatca agctggacat
    52201 ggaggttcct tataagcctt caattacaga gtattatcgg cagcgacccg ccggacttga
    52261 gttattcaga acagattatc aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc
    52321 gatttcatct gctggatcat gttggtgagt ttacgggcat agtgaggatc ggtagcatag
    52381 cccgcgtcct gtaggacctg cgccccctgt tccgcactcg cggcggtcgt cacggcggcg
    52441 tagcgcgggt tacgtgttaa cagcccaacg taatccgaca atgcttccag atacgagctg
    52501 tagacccgaa acttcgcttt tactttcttc gcttcgccgt tttcatattc agtcgtggtg
    52561 atttcagtga cttgcccttt ccagttgcca gaggctttga caccaaacag gttatagctt
    52621 ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac cagattcaag cgcagcctga
    52681 gcgaggatca aatgatgtgg cacaccgctt tgctggcttg ccagttgggc gggcagcgag
    52741 agttgcgcga ggaatgctct actgtcaccc ggcagcgaat catcgtagtt acgtggtacg
    52801 gccttttgca ccagctgcga aagcgtctgg ttttgataac gcaccacggt ttcgagcggg
    52861 aatttcatcg gtgctgctgg cgtggactcc tctggcaatg gttgttctgg cgtcatctgt
    52921 ttaaccatca tctctgcaag ccccagacct ttgcccgccg tcatctgttg ggcaatttgc
    52981 tggtcataca tactggtata cagacgagtg tgctcgctgc tgaacaggcc atcttttggt
    53041 aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca tcccttccac ctgacgggcc
    53101 accggacgga tatttgccgc cggatcttcg ctcgctttcg cctttagttc gttgagtgat
    53161 tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc tgatcatcag atgatttcca
    53221 gttttgcccg cagacatccc gcactttgca ttgattgcaa aatagacatc agatccatcg
    53281 gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt gaggctggcg ctggaacgta
    53341 cgctttgcag cgaaccgccg ctctggcgta aatcgatctg cgtttgtgga gtaacaacag
    53401 tctgtccacc accaaacggt gtatctggct ggctgacatt ggcctgacga ttaactgtta
    53461 ctgagagatt tccctgcgct accgcgcagc tgtcgagggt cacttcgcga ttcatcacca
    53521 ccgaaccggt gcgcgagtta atcactactt tagcgtcctg cggggtgaca ttaacctgca
    53581 tattctggat atcggcaagg aaacggacct gggaactgtt gccacttggt acgcgcacct
    53641 gaatagtccg cgcatctaac gcggtggcgc tgccatatcc acgcacgcgg ttgatggtgt
    53701 cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc
    53761 cgacgccaaa ctggctgggc aattcacgtt caataaccgc accattggtg atccgtccac
    53821 cgttcagttg gttaacctga acactgctac cgcctgcgga ggctcctgcg ccgccaacca
    53881 gaatattgcc ctgcgccagc gcatacacct gactgtcaac gcccttaagc ggtgtcatca
    53941 acaacgtacc tccacgcaag cttttggcat tacccatgga agaaaccacc acatcgatgg
    54001 tttgcccctg acgtccaaac ggaggaagtg acgctgtcac cattaccgca gcgacgtttt
    54061 ttagctgcat attggtgccc gtcggaacgg taattcccag ctgtgagagc atgttattaa
    54121 gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc agtgccatcc agccccacta
    54181 ccaagccata gccaatcagt gagttttgcc ttaccccctg aacactggtg agatcgcgaa
    54241 tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat taatgcagag agaaatttaa
    54301 tcaccacagc ctcacttaca ttggcgacag gttaaggaag aaacgctgca accagcccat
    54361 attttgcgct tcgttaatgt agccattgcc tacgtattca atacgcgcat ccgccacctg
    54421 agtagacggt acggtattgc tgccgctgat agtgcgtgga ttaaccacgc ccgagaagcg
    54481 aataaattcg gtaccctgat taatggcaat ctgtttttca cccaccacat gcaggttgcc
    54541 gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg ctaaaggtat tgctggcatt
    54601 ggccccgccc tttccgttga acgtgttacc accggaggct tcgacatcgg cacgagcgtt
    54661 accaaacagc ccctgcaaat agcgcggcac agtatcaaag ccaaaattag ttttaccgtc
    54721 acggctggca ttcgcagagg agcttttgct ggcgctgacg ttctcctgca acacgatggt
    54781 cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac agcggttgat agccatagtt
    54841 aatcggctga gcagactgga aaatagaacc gttggcgacg ggcgtcggac cgggaaccgg
    54901 ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt atccaggcgc agccggttag
    54961 tgaaagcacc aacaagctgg aaatggcata agtatgcgca gcgttttttt gcatggctat
    55021 catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt tatgccttag agttgcgtca
    55081 gtttttgcag catctgatcg gtggtggaca ccgctttact gttgatttcg taagcgcgtt
    55141 gcacctgaat catattgacc agttcttccg ccacgttgac gttagacgtt tcaacatacc
    55201 cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct ttcgttcggt gcaccggagg
    55261 attgcgtttc ggtgtagagg ttttcgccaa tgctctccag tccggtatca ttcataaagg
    55321 tggtgagatt gagctgccca acctgaaccg gagctgcctg gccttgttgg gttacgctga
    55381 ccacgccatc acgaccgatg gtgatactta acgcattcgc cggaatggtg atcgctggct
    55441 gcacctgaaa accaccagcc gtcaccagct gcccgttctg atccacctgg aaagagccgt
    55501 cacggatata ggctgatgag ccatctggca acattacctg gaaaaagccc tgccctttaa
    55561 tcgcgacatc tttgctgtta ttggtctgcg acagattgcc ctggctgtgt aagcgttcag
    55621 tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga gggtaaggtg gtttgttcgg
    55681 aagactgtgc ccccggctgg cgaatggttt gataaagcag atcttcaaac accgcgcgct
    55741 gacgcttaaa accgttagta ctgacgtttg ccaggttgtt ggcaatgacg tccatattgg
    55801 tttgctgggc gtcaaggccc gttttggcga tccataatga actgatcata taatgtcctg
    55861 tagtggattg ctgacaagca gagcagaagc acgcgacagt gtgaatttcc cctcacccta
    55921 accctctccc caaaggggcg agggaatagt ccgcgcgctt ttattgcaat gtcatcaatc
    55981 gttcaattaa ctcatcgaca gcagttggtt ggcacggcct gcgttatcat cgacgctgct
    56041 gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc tcattgccgc
    56101 aacggcattg acgttactgc cttccagaac ccccgacatc acacgcaagg ttggatctgc
    56161 ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt aaacgaaaaa tgccgtcgtc
    56221 accgcgctgc acttcgctgc ccgtggcttt caccagtttc agacgcccta ctggcgcaac
    56281 tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg ccatcggcag caatcgtgat
    56341 ttccgcccct tccggcacag caattggccc cgcctcgcct atcaccggat gcccctgaat
    56401 tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc cttcgctgcc
    56461 gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg
    56521 taatccatct tgccgggcgt catgtctgca cccggtgttg acgccgttac cagcgtgcgc
    56581 gtgggcagtg aaagcccttc caccggcact gcgcgcaagg cgtttaactg cgcgcgaaaa
    56641 ccgggcgtag aggcattggc cagattactg gcggttaccg cctgttgatt cagcgtctgg
    56701 ctggctgctc ccatcgcagt ataaattgcg tgatccattg agctatcccg tcagcgatta
    56761 gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt ttgatggtct gggcgttaga
    56821 cttatagtta cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga
    56881 cgcttccagc gcaccgttgg tcagggtgcc aaagtttccc gtcccggctg tccccaacag
    56941 cgccacgcca gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc
    57001 gttgttggca aagttcgcca gtacaatctg ccccagcagt tgggtttgtt cgttggaatt
    57061 gttgccgaca accgtaccgt catcattgat ttgataactc accagatcgc ccggtttgta
    57121 gccattctgg gtggttgcca caatgttgtt agcgccggta ttttgctgca tggagttgag
    57181 gaagctcaga ctaaacgtgg cgggttctgc accgttaatt gcgccggttg cgatattatt
    57241 cgccatcgca ccatccacta atgtgccatt agcattaaat tccagtgttg tcgctgtctt
    57301 cgcaatgctg tttggatcac tgctatcctg ggtgtagacc tgccagttat tatccccggt
    57361 cttcacaaaa tagacgctca tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga
    57421 accttttttg ttatagctat ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg
    57481 atcactggaa ttcaggttga tctgcatcga cgccgtggtg gtagttttcg ctgccatcag
    57541 ggtattcggg atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc
    57601 ggttaccggg taacccgtca gctgtaaacc ttgcgtattc aacaggttac ggttttcatc
    57661 cagcttaaat tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa
    57721 aaaaccgttc tggctgatag caacgtccag acctcgcccg gtgttggtgg tcgtgccatc
    57781 ggtaaagtcc tgagtgatac cggcaacttt tacccccagt cccactttcg aaccggcaaa
    57841 catatcggca aaagaggccg tgcctgattt aaagccgtag gtggcggagt tggcgatatt
    57901 gttgccaata acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa
    57961 ggccatgact gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt
    58021 ggtggtgccg taagtgccga gatccagcgt attaccgctg ttgccgcgga tcacaccctg
    58081 caccagagca aactgcagcg gctgggcaac cagttgtgta ccaccgttac tggcgctaat
    58141 cgctacattg taagaaccgt tcggcgcagt gctgccatca gtcaacgaac cgtcccaggt
    58201 gaaactgtga actccggcgg tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt
    58261 tttatcggtg atggtggccg taactttgtc tgccgcctgt tgcagctcaa caccaaacgg
    58321 cgtggtcgtg gtcacagccc cttcttcact gccggttccc gcaagaacag ttgtgccggg
    58381 gatcatcacg ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg
    58441 tccggaaata gatccgagcg tggtattgag tttttcaatc ccgctgaccg tgctgatttg
    58501 tgccaattgc gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag
    58561 ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag
    58621 cgaactactg ctggtggtac tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat
    58681 ggacatagct ttctccttta ttgaccgagc gtaagggttt tcagcatcat gcttttcacc
    58741 gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc
    58801 tccccgacga catcaacgtt cggcattttt acgtagccct ttgcatctgc cagcggatta
    58861 cctggttcat aaaccagttt gtccggggcc tgactttctt aacatcggca acctttacgc
    58921 cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg
    58981 gctgtccatc aggaccggtc acgctatcag catttgccag attactggcc gccacgttca
    59041 ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca
    59101 tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc
    59161 taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat
    59221 cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag
    59281 gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt catcgtcagt gcaaccacac
    59341 tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc
    59401 gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct
    59461 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
    59521 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
    59581 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
    59641 tacaattcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg
    59701 cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca tcgcaattgc
    59761 acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
    59821 cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc aataattccc
    59881 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
    59941 ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcctattgtg cggggaggaa
    60001 agctggaagc tggcaatgtc aaactgaaac gtggacgcct ggataccctg ccaccgcgta
    60061 cggtgctgga tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc
    60121 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
    60181 atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
    60241 cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg
    60301 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
    60361 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg
    60421 atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc accgacgcgc
    60481 cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa
    60541 gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg
    60601 aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg
    60661 atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt
    60721 gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa
    60781 gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa
    60841 caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa gaaagaaccc
    60901 aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag
    60961 caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat
    61021 caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt
    61081 cagacctcaa caccccatcg cggcagtaaa aagatttcga tctgaatatt atctccggcc
    61141 tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa
    61201 gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa
    61261 acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc
    61321 taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca
    61381 gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt
    61441 cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa
    61501 cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt
    61561 aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa
    61621 acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt
    61681 accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact
    61741 cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt tgtattcatc
    61801 atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac
    61861 gcccagcaca ccggagggaa actccattaa gcggtcggcg taatacatcc aagacaccga
    61921 accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga
    61981 gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccag catcgtggaa
    62041 gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag
    62101 ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta
    62161 cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg gcgcaaacgc
    62221 cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat
    62281 cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc
    62341 tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc
    62401 gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga caaagacccg
    62461 cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga
    62521 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
    62581 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
    62641 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
    62701 cggtgttcta atccggaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
    62761 atagtctacc tagattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
    62821 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cactgcctat tcgccggcgg
    62881 tttgcggaac tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc
    62941 cacgttgctc aagagtgctc tgccaaccag gaatcggttt attcactaca ccctgcccgc
    63001 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct
    63061 gcacggtttc acgctgactt ccggcacggc gatgcataca agtggtgatc tgcaaaggac
    63121 cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac
    63181 cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat
    63241 aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat
    63301 ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa ctcaccgtag agtggtgcga
    63361 aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc
    63421 tttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat
    63481 tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggagtgcaca aaaaccgcat
    63541 cacaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag ctgtcacaaa
    63601 ttggcagggc tttggcgcgc gtaggcgacc aggctccttg taacgtccag tcagacgctg
    63661 ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccact acgccgatac
    63721 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ttgtttcagt
    63781 tctgccactt cgatttccag ggcttcgacg cgggtctata aatcaccgtc aaccatgttc
    63841 gacatagccg ttaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg
    63901 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc
    63961 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg
    64021 gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac
    64081 gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
    64141 tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga
    64201 ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttt acggttcgtt
    64261 ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc
    64321 acctgctttt ccagcaagca accaattacg cgagcttcca aggcagtaag ttgatatttc
    64381 atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg
    64441 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
    64501 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
    64561 atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag
    64621 tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc
    64681 gcgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa
    64741 gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccta gcctggcctg
    64801 ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg
    64861 gcgattctct gcgtaataat ccgccagacg ccaggcatca cgatcatgca ccagacgcac
    64921 gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct
    64981 atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac
    65041 cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac
    65101 tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct
    65161 tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg
    65221 cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt
    65281 gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg
    65341 ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg
    65401 tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat
    65461 gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg
    65521 gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct
    65581 ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt tgatgatgca
    65641 ggacagtgcc agtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt
    65701 tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta tgtgcggcgt tcaatgcgtg
    65761 gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg
    65821 cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct
    65881 ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcgccctc
    65941 tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat
    66001 cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat
    66061 aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctacaacaac ttttcaccct
    66121 gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc
    66181 ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc
    66241 gattggcggc gctattggtt atatcggtgg tggctggctg tttgacctgg gcaaatcggc
    66301 gcaccagcca gagcttccgt ggatgatgct gggaattatt ggcatcttca ctttccttgc
    66361 gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt ttgctggaac gcgacgcctg
    66421 atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag
    66481 tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt
    66541 cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc
    66601 cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat
    66661 gccagaaagc atggatatcg ttcactatgt cgataaactc gacggcaaac cgttactgac
    66721 cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa
    66781 actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc ccgccgcgcg
    66841 caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca
    66901 ctctgacggt ctgattaaga atatcagcga tgatttgcgt gcgctggaca aactgatcgt
    66961 caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctact
    67021 gcgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgcta attaccgcga
    67081 taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc
    67141 cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt
    67201 tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga
    67261 ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct
    67321 gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac
    67381 ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga
    67441 caaacttgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg
    67501 ccaatctgga catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga
    67561 aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg
    67621 tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt
    67681 tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg
    67741 gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg
    67801 aaattgtcat tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt
    67861 gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag
    67921 catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc
    67981 accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt
    68041 atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg
    68101 tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg gcacgatttc accccattga
    68161 tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaatgaag
    68221 ccgtgttcac cgctgcaaaa ctttacccgg caaacgcaac cactaactcc agccacggcg
    68281 tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc
    68341 tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct
    68401 ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg
    68461 agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg
    68521 ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc
    68581 gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg
    68641 aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac
    68701 gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg
    68761 gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt
    68821 ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc gaactggtac
    68881 gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc
    68941 tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca
    69001 ttaggttatt ttacctgtat aaataaccag tatgttcaac agggggctat tatgcgaatt
    69061 gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc
    69121 gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt
    69181 tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt
    69241 agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat
    69301 gcagtgattt ttgtttttgc cggatcgccc cggctttttt attttactta cgaattttga
    69361 gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga
    69421 catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt ccccccttta
    69481 aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat
    69541 tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa
    69601 tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga
    69661 tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga
    69721 agttggtcag acgctctggt taaccactga tgttgccaga caatttattt cgatattaga
    69781 agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta
    69841 acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg
    69901 tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa
    69961 atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac
    70021 ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag
    70081 ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc
    70141 gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg aagatgatcc
    70201 catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat ttctcgccaa
    70261 cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat
    70321 tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga
    70381 ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc
    70441 gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt
    70501 aactattgaa acggttgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac
    70561 atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag tatttgcctg
    70621 gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact
    70681 gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa
    70741 acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggc
    70801 cagtgatggg tcagaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg
    70861 cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac
    70921 cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt
    70981 tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gaccaaaaaa gcgattttga
    71041 tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg
    71101 ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc
    71161 gagcatttga tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat
    71221 gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca
    71281 tttagcgtga aaaattgaat gatattattc ctgcttctac agcactcacc agacatctct
    71341 gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa ttattgaaat
    71401 aaaaataaat atcattcaat ttgttatttt tgtgttgcga ttcattctcc gacacgccat
    71461 tttctttctc tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac
    71521 gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt
    71581 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac
    71641 ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt
    71701 ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg
    71761 ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct
    71821 atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
    71881 cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg
    71941 cgtggagcgt cgtagtactg tctgttatgc acggatttat ggccctgaag catcatttca
    72001 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
    72061 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt
    72121 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaacttcc
    72181 gcatacagca tcttggctat agctggttat atggcacctt taaagatttc gacggtactt
    72241 ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca
    72301 gcgtcgatac taaccacgcc gaacgcgata aacatcttcg cagtgcggat tttctcaata
    72361 ccacaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac
    72421 tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga
    72481 aattaattgg tcagggcgac gacccatggg gcggtaaacg tgcaggcttt gaggccgaag
    72541 gcaaaattaa gctcaaagac ttcaatatca agacagattt aggtccagct tctcaggaag
    72601 tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat gttgccggat
    72661 ggcacgcatc cggcaatgat atttattctg ttggatcagg aatacccagt gttgtattca
    72721 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
    72781 gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
    72841 atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt
    72901 tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata
    72961 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc
    73021 cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt
    73081 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
    73141 acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
    73201 cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat
    73261 agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg
    73321 ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg
    73381 caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca
    73441 acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa
    73501 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
    73561 acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct
    73621 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcag
    73681 ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
    73741 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
    73801 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg
    73861 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
    73921 acatacaatt gcccgtatag gttgaaaaac aggattgata tgacgaatct acccaagttc
    73981 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
    74041 ttggtcgtgc aattgcccta cccgattatc taccgcctcg gttgtggatt aggaaaactg
    74101 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
    74161 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
    74221 atgggcctga tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg
    74281 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
    74341 ttagttggca tccattttct gacactggag ctgggcgcgc ggcagtttgg tatgcaggaa
    74401 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
    74461 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
    74521 gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc atgattacgg cccgcgctca
    74581 agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg
    74641 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
    74701 aaagggtatc aattgattat gctgccacca gagtgttctc cgccactgga tgatgccgaa
    74761 actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
    74821 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
    74881 atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat
    74941 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgtt
    75001 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
    75061 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
    75121 tttcttaccg gggccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag
    75181 cttggtgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt cagtataatg
    75241 ggttcctaaa cacatcattt taggtattga atatgagacg ggcaatcagc tacatcagat
    75301 tttcaagtga acgacaactg aaaggggact ctgtacgccg acaatctaag ctggttactg
    75361 actggcttga taagaaccct gagttttact tggattcatc cttgagcttt aaagatttgg
    75421 ggaaaagtgc ttttagtggt aagcacctga aaggtggtct aggtgacttt ttaaccgcta
    75481 tcgagaaagg gcttgttaaa gctggtgata cgctactaat cgagagttta gaccgtctgt
    75541 cacgtcagga tattgatatt gcttctgagc tattacgccg tatcttaagg gctggtgtag
    75601 atgtagtcac actgagcgat ggagaacatt acacacgaga aagtttaaaa gacccgttag
    75661 ccctgattaa aagcattctg ataatgcagc gggcgcatga ggaaagtttg agaaagtctg
    75721 agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat ctccgaaggt atcaaggtat
//
LOCUS       DAJTNW010000005        75611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNW010000005 DAJTNW010000000
VERSION     DAJTNW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(435..641)
                     /locus_tag="OO179_000336"
     CDS             complement(435..641)
                     /locus_tag="OO179_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5530537.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO179_000337"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO179_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5530538.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO179_000338"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO179_000338"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5530539.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO179_000339"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO179_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO179_000340"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO179_000340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5530540.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO179_000341"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO179_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="OO179_000342"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="OO179_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5530541.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="OO179_000343"
     CDS             8989..9093
                     /locus_tag="OO179_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5530542.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="OO179_000344"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="OO179_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="OO179_000345"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="OO179_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5530543.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OO179_000346"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OO179_000346"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5530544.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OO179_000347"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OO179_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5530545.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="OO179_000348"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="OO179_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5530546.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="OO179_000349"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="OO179_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5530547.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="OO179_000350"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="OO179_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5530548.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="OO179_000351"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="OO179_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5530549.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="OO179_000352"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="OO179_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5530550.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="OO179_000353"
     CDS             complement(18055..18381)
                     /locus_tag="OO179_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530551.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OO179_000354"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OO179_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5530552.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="OO179_000355"
     CDS             complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="OO179_000355"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5530553.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="OO179_000356"
     CDS             complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="OO179_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5530554.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24549..25349
                     /gene="nagB"
                     /locus_tag="OO179_000357"
     CDS             24549..25349
                     /gene="nagB"
                     /locus_tag="OO179_000357"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5530555.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25409..26557
                     /gene="nagA"
                     /locus_tag="OO179_000358"
     CDS             25409..26557
                     /gene="nagA"
                     /locus_tag="OO179_000358"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5530556.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26566..27786
                     /gene="nagC"
                     /locus_tag="OO179_000359"
                     /gene_synonym="nagR"
     CDS             26566..27786
                     /gene="nagC"
                     /locus_tag="OO179_000359"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5530557.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27834..28586
                     /gene="nagD"
                     /locus_tag="OO179_000360"
     CDS             27834..28586
                     /gene="nagD"
                     /locus_tag="OO179_000360"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5530558.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28983..30647
                     /gene="asnB"
                     /locus_tag="OO179_000361"
     CDS             28983..30647
                     /gene="asnB"
                     /locus_tag="OO179_000361"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5530559.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31026..31102
                     /locus_tag="OO179_000362"
     tRNA            31026..31102
                     /locus_tag="OO179_000362"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31060..31062,aa:Met,seq:cat)
     gene            31111..31195
                     /locus_tag="OO179_000363"
     tRNA            31111..31195
                     /locus_tag="OO179_000363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31145..31147,aa:Leu,seq:tag)
     gene            31219..31293
                     /locus_tag="OO179_000364"
     tRNA            31219..31293
                     /locus_tag="OO179_000364"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31251..31253,aa:Gln,seq:ttg)
     gene            31328..31402
                     /locus_tag="OO179_000365"
     tRNA            31328..31402
                     /locus_tag="OO179_000365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31360..31362,aa:Gln,seq:ttg)
     gene            31418..31494
                     /locus_tag="OO179_000366"
     tRNA            31418..31494
                     /locus_tag="OO179_000366"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31452..31454,aa:Met,seq:cat)
     gene            31543..31617
                     /locus_tag="OO179_000367"
     tRNA            31543..31617
                     /locus_tag="OO179_000367"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31575..31577,aa:Gln,seq:ctg)
     gene            31655..31729
                     /locus_tag="OO179_000368"
     tRNA            31655..31729
                     /locus_tag="OO179_000368"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31687..31689,aa:Gln,seq:ctg)
     gene            complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="OO179_000369"
     CDS             complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="OO179_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5530560.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33204..34628
                     /gene="miaB"
                     /locus_tag="OO179_000370"
     CDS             33204..34628
                     /gene="miaB"
                     /locus_tag="OO179_000370"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5530561.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34781..35821
                     /locus_tag="OO179_000371"
     CDS             34781..35821
                     /locus_tag="OO179_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5530562.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35818..36285
                     /gene="ybeY"
                     /locus_tag="OO179_000372"
     CDS             35818..36285
                     /gene="ybeY"
                     /locus_tag="OO179_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5530563.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36375..37253
                     /gene="corC"
                     /locus_tag="OO179_000373"
     CDS             36375..37253
                     /gene="corC"
                     /locus_tag="OO179_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5530564.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37278..38816
                     /gene="lnt"
                     /locus_tag="OO179_000374"
     CDS             37278..38816
                     /gene="lnt"
                     /locus_tag="OO179_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5530565.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39213..40121
                     /gene="gltI"
                     /locus_tag="OO179_000375"
     CDS             39213..40121
                     /gene="gltI"
                     /locus_tag="OO179_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5530566.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40291..41031
                     /gene="gltJ"
                     /locus_tag="OO179_000376"
     CDS             40291..41031
                     /gene="gltJ"
                     /locus_tag="OO179_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5530567.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41031..41705
                     /gene="gltK"
                     /locus_tag="OO179_000377"
     CDS             41031..41705
                     /gene="gltK"
                     /locus_tag="OO179_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5530568.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41705..42430
                     /gene="gltL"
                     /locus_tag="OO179_000378"
     CDS             41705..42430
                     /gene="gltL"
                     /locus_tag="OO179_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5530569.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42548..43483
                     /gene="rihA"
                     /locus_tag="OO179_000379"
     CDS             42548..43483
                     /gene="rihA"
                     /locus_tag="OO179_000379"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5530570.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43567..45228
                     /gene="hscC"
                     /locus_tag="OO179_000380"
                     /pseudo
     CDS             43567..45228
                     /gene="hscC"
                     /locus_tag="OO179_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="OO179_000381"
     CDS             complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="OO179_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5530571.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46736..47443)
                     /locus_tag="OO179_000382"
     CDS             complement(46736..47443)
                     /locus_tag="OO179_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5530572.1"
                     /translation="MNNEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47545..48099
                     /locus_tag="OO179_000383"
     CDS             47545..48099
                     /locus_tag="OO179_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5530573.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48109..49536)
                     /locus_tag="OO179_000384"
     CDS             complement(48109..49536)
                     /locus_tag="OO179_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5530574.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49533..50240)
                     /locus_tag="OO179_000385"
     CDS             complement(49533..50240)
                     /locus_tag="OO179_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5530575.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50404..51381
                     /locus_tag="OO179_000386"
     CDS             50404..51381
                     /locus_tag="OO179_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5530576.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51451..51933)
                     /locus_tag="OO179_000387"
     CDS             complement(51451..51933)
                     /locus_tag="OO179_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5530577.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52168..54750
                     /gene="leuS"
                     /locus_tag="OO179_000388"
     CDS             52168..54750
                     /gene="leuS"
                     /locus_tag="OO179_000388"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5530578.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFIPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54765..55346
                     /gene="lptE"
                     /locus_tag="OO179_000389"
     CDS             54765..55346
                     /gene="lptE"
                     /locus_tag="OO179_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5530579.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55346..56377
                     /gene="holA"
                     /locus_tag="OO179_000390"
     CDS             55346..56377
                     /gene="holA"
                     /locus_tag="OO179_000390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5530580.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56379..57020
                     /gene="nadD"
                     /locus_tag="OO179_000391"
     CDS             56379..57020
                     /gene="nadD"
                     /locus_tag="OO179_000391"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5530581.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57044..57655
                     /locus_tag="OO179_000392"
     CDS             57044..57655
                     /locus_tag="OO179_000392"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5530582.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57915..58232
                     /gene="rsfS"
                     /locus_tag="OO179_000393"
                     /gene_synonym="rsfA"
     CDS             57915..58232
                     /gene="rsfS"
                     /locus_tag="OO179_000393"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5530583.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58236..58703
                     /gene="rlmH"
                     /locus_tag="OO179_000394"
     CDS             58236..58703
                     /gene="rlmH"
                     /locus_tag="OO179_000394"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5530584.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58734..60635
                     /gene="mrdA"
                     /locus_tag="OO179_000395"
     CDS             58734..60635
                     /gene="mrdA"
                     /locus_tag="OO179_000395"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5530585.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60638..61750
                     /gene="mrdB"
                     /locus_tag="OO179_000396"
                     /gene_synonym="rodA"
     CDS             60638..61750
                     /gene="mrdB"
                     /locus_tag="OO179_000396"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5530586.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61761..62849
                     /gene="rlpA"
                     /locus_tag="OO179_000397"
     CDS             61761..62849
                     /gene="rlpA"
                     /locus_tag="OO179_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5530587.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62988..64199
                     /gene="dacA"
                     /locus_tag="OO179_000398"
     CDS             62988..64199
                     /gene="dacA"
                     /locus_tag="OO179_000398"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5530588.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64310..64573
                     /locus_tag="OO179_000399"
     CDS             64310..64573
                     /locus_tag="OO179_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5530589.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64674..65315
                     /gene="lipB"
                     /locus_tag="OO179_000400"
     CDS             64674..65315
                     /gene="lipB"
                     /locus_tag="OO179_000400"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5530590.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65574..66527
                     /locus_tag="OO179_000401"
     CDS             65574..66527
                     /locus_tag="OO179_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5530591.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66736..67701
                     /gene="lipA"
                     /locus_tag="OO179_000402"
     CDS             66736..67701
                     /gene="lipA"
                     /locus_tag="OO179_000402"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5530592.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="OO179_000403"
     CDS             complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="OO179_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5530593.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68134..68922)
                     /locus_tag="OO179_000404"
     CDS             complement(68134..68922)
                     /locus_tag="OO179_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5530594.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69015..69398
                     /gene="crcB"
                     /locus_tag="OO179_000405"
     CDS             69015..69398
                     /gene="crcB"
                     /locus_tag="OO179_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5530595.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="OO179_000406"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="OO179_000406"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5530596.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="OO179_000407"
     CDS             complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="OO179_000407"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5530597.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70984..72369
                     /gene="dcuC"
                     /locus_tag="OO179_000408"
     CDS             70984..72369
                     /gene="dcuC"
                     /locus_tag="OO179_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5530598.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLDRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72410..72751)
                     /locus_tag="OO179_000409"
                     /pseudo
     CDS             complement(72410..72751)
                     /locus_tag="OO179_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73089..73598)
                     /locus_tag="OO179_000410"
     CDS             complement(73089..73598)
                     /locus_tag="OO179_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5530599.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73761..74006
                     /locus_tag="OO179_000411"
     CDS             73761..74006
                     /locus_tag="OO179_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5530600.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73981..>75611
                     /locus_tag="OO179_000412"
     CDS             73981..>75611
                     /locus_tag="OO179_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5530601.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHSTV"
BASE COUNT        18609 a        18989 c        19203 g        18810 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat
    20521 gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc
    20581 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20641 agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20701 acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20761 cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc cttaatgacg
    20821 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    20881 tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac ctgacggctc
    20941 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctgatag
    21001 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg
    21061 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    21121 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    21181 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    21241 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21301 tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag acgacggttg
    21361 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    21421 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21481 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21541 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc
    21601 gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21661 gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21721 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21781 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    21841 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21901 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21961 ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag atcttcatcg
    22021 atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    22081 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    22141 gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg
    22201 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca
    22261 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22321 agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22381 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
    22441 tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22501 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    22561 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22621 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22681 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    22741 tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag ctgacgcagc
    22801 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22861 gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg
    22921 tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat
    22981 acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    23041 cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga tctcgtcttc
    23101 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    23161 gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt tctggctggc
    23221 ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag agaagccggc
    23281 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23341 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23401 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    23461 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23521 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    23581 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23641 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23701 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23761 ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac
    23821 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23881 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23941 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    24001 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
    24061 gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    24121 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    24181 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    24241 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg atgttctgta
    24301 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta
    24361 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24421 ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg cctgtctgcg
    24481 caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt tacttattga
    24541 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24601 gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg tttgtactgg
    24661 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24721 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24781 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24841 atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc gatgccgagt
    24901 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24961 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    25021 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    25081 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag
    25141 aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc gccgttgaag
    25201 gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa gcgatcatgg
    25261 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25321 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc cttgctcagg
    25381 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25441 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25501 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25561 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
    25621 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25681 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
    25741 gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25801 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25861 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
    25921 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25981 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    26041 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    26101 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
    26161 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
    26221 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    26281 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26341 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    26401 gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac gtctcggcac
    26461 gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa
    26521 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26581 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26641 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    26701 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26761 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26821 ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct gtttgatctc
    26881 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26941 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    27001 gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    27061 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    27121 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
    27181 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    27241 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    27301 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27361 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27421 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27481 aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc
    27541 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg tgaaatcacc
    27601 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca agcgctgaag
    27661 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    27721 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27781 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27841 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27901 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27961 actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
    28021 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
    28081 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    28141 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca
    28201 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    28261 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
    28321 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
    28381 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc ggcgataacc
    28441 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28501 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
    28561 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
    28621 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
    28681 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    28741 caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa taaaacccgc
    28801 taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt caccaatcga
    28861 caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca gtgctgcttt
    28921 gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg
    28981 ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga
    29041 aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg
    29101 ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg
    29161 cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt gaaatctaca
    29221 accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact
    29281 gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg
    29341 gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc
    29401 atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg tatgtggcct
    29461 cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct
    29521 atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg
    29581 atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg
    29641 ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt ggtctggatt
    29701 cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac
    29761 gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg
    29821 atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact
    29881 tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg
    29941 atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga
    30001 tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt
    30061 acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg
    30121 ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag
    30181 cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg
    30241 ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat
    30301 acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca
    30361 gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa
    30421 ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg
    30481 agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt
    30541 ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa aaaatggacg
    30601 atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcggtact
    30661 gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc tgatatgcag
    30721 taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta
    30781 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa
    30841 agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa tatagcgcca
    30901 cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg
    30961 acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa
    31021 aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    31081 tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt agacgcacca
    31141 gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac
    31201 cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg
    31261 cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg gttcaccgcc
    31321 cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    31381 tcgaatccag gtactccagc catcgaagaa ataatctggc tacgtagctc agttggttag
    31441 agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accaaattct
    31501 gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat cgccaagcgg
    31561 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt
    31621 tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc ggtaaggcac
    31681 cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac attaaaaaag
    31741 ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc gatgttgaca
    31801 cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct acggctggca
    31861 cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc tgacgtttca
    31921 acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc agcggcggca
    31981 gattattact gaatccggca taaaacagat ccataccact ttgcataatg aagttatccg
    32041 ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct tcaccgtagc
    32101 tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca agattcaccc
    32161 cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca agccccggct
    32221 gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca agcggtgtaa
    32281 cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc gccatattca
    32341 tattctgcaa ctggcgaata cgcgccggag agtcatacca caccagcgat gcccagttat
    32401 caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc
    32461 cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac tgccaggcat
    32521 gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca ccaatcacca
    32581 gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca ttatctcgat
    32641 gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct tccagcgcct
    32701 gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac ggcaacttta
    32761 attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc agtctgcggt
    32821 aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta agcaatgata
    32881 ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg acaaacggtg
    32941 caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc cccagcgcca
    33001 gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga tttgtcatgg
    33061 tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc aagggctgat
    33121 aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg cattcctggc
    33181 tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc tggggctgtc
    33241 agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc cacggctatc
    33301 aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc tcaatccgcg
    33361 agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta aaagagaaga
    33421 atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc gagcacattc
    33481 gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac cgtctgccgg
    33541 agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc ttcccggaaa
    33601 tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca
    33661 tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc cgtggtgaag
    33721 aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg gctcagggcg
    33781 tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag aactacgacg
    33841 gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac gggatcgatc
    33901 gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc gaagtgtacc
    33961 gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt tccgatcgca
    34021 ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc atccgtaaac
    34081 tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc ttccctggcg
    34141 aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat ttcgacatga
    34201 gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg gttgatgatg
    34261 ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc aatcagcaag
    34321 cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta gaagggactt
    34381 cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg gtcaacttcg
    34441 agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac gtctacccga
    34501 actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc atggcagaaa
    34561 caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg ggttattatc
    34621 agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct ttatccccat
    34681 ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta taggccggtg
    34741 aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag acactcgcga
    34801 aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc cgtttgatga
    34861 caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg ataaccactt
    34921 taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc gtagcctgta
    34981 tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac agatccacct
    35041 tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg agtacggcaa
    35101 agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa accaggcgca
    35161 gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg cgggtacggg
    35221 taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg
    35281 tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga
    35341 tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt ttgaaatgct
    35401 gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg cgccgctggc
    35461 ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga gccagaacac
    35521 taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa aagcggttat
    35581 caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct tacgtcatgc
    35641 cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca gcgaagacgt
    35701 ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg aagaagcaga
    35761 acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg aacaaaaatg
    35821 agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt accggaagag
    35881 agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga gtcggaagtg
    35941 actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta tcgcggtaag
    36001 gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat ggagatgtcg
    36061 ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca ggagcaaggc
    36121 aaaccactgg aggcgcattg ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt
    36181 tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac agagattatg
    36241 cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc caggcatttg
    36301 actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac taacatgaga
    36361 acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata agcaacaaga
    36421 agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg
    36481 aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa gatacgcgcg
    36541 atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc atgatccccc
    36601 gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt gatgtcatca
    36661 tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac attgaaggga
    36721 ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg
    36781 acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga
    36841 aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc ggtggggttt
    36901 ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt gaagacgagt
    36961 atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg accgtgcgcg
    37021 cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc gatgaagaag
    37081 tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg cgtggcgaaa
    37141 ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg cgtattattc
    37201 aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa taaaaccgaa
    37261 actggataga taactacatg gcttttgcct cattaattga acgccagcgt attcgcctgc
    37321 tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac gacgtctggc
    37381 ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt cgcccactcc
    37441 agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt attaactggg
    37501 tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc ttcctggtgg
    37561 tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgtc
    37621 tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc tggcaagtga
    37681 ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc ggctatagcc
    37741 agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc attaacttcc
    37801 tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac tggcgtccgc
    37861 tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc caatggttta
    37921 ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt ccgcaatcgc
    37981 tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac gcaacggcac
    38041 cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc gatctggaaa
    38101 ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa ggtagctcgc
    38161 tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat acctacaaca
    38221 ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat cgctataaca
    38281 aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg cgtccgttag
    38341 caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc cagccgccgc
    38401 tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt ctcgccgagc
    38461 aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc aacgatgcgt
    38521 ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg cgtgcgctgg
    38581 agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg attggcccgc
    38641 agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc actaacgtga
    38701 cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg tgggtgctga
    38761 cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt aaataatccc
    38821 ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc ggcatcagtg
    38881 cattatccgg ataacaatat tctcctatcc aactttggca catctattgc tttgttatac
    38941 aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat ccaccggtag
    39001 caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac ctcgcaaaaa
    39061 caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc taacaggcac
    39121 aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg ggtatccatg
    39181 cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct gccacagcaa
    39241 tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcag
    39301 gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt
    39361 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg caggattact
    39421 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac
    39481 tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
    39541 aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
    39601 ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa gattttgccg
    39661 acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
    39721 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    39781 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gctctgctgg
    39841 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc aagccgcagt
    39901 ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    39961 atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt
    40021 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    40081 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    40141 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc
    40201 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    40261 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    40321 ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag tggttttcag
    40381 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    40441 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    40501 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag
    40561 ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt
    40621 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    40681 cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc gatgggcctg
    40741 acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    40801 ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gaccatcggt
    40861 ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag
    40921 tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat gctggtgatg
    40981 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    41041 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcaccc
    41101 tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg ctggcggtga
    41161 tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt aacgtattcc
    41221 gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg ggatttctgc
    41281 aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg atggtggcgt
    41341 tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt
    41401 cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag tcgatgaaac
    41461 tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact cagggcatcg
    41521 tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    41581 cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt gccggatttg
    41641 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    41701 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    41761 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg
    41821 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac
    41881 cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gctcccgcgt
    41941 cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac
    42001 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa aagcattgaa
    42061 attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg
    42121 cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta ttgcgatgct
    42181 gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    42241 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    42301 ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg aagactcgcc
    42361 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    42421 cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc tttatcgttt
    42481 cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg
    42541 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg acgctatcgc
    42601 aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    42661 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    42721 caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc
    42781 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc
    42841 accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa gtgcggaacc
    42901 tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc
    42961 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    43021 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    43081 ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag cgcaaatcca
    43141 cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    43201 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    43261 gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta ccactgttga
    43321 gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg ttgttgatta
    43381 ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg atcgtcaggg
    43441 ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt cattgattaa
    43501 gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt tcaagatata
    43561 aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac caatagttta
    43621 attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta
    43681 acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta
    43741 tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagc
    43801 tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt attacgctca
    43861 ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt gatctccgtt
    43921 ccggcttatt tcagtgatga acaacgcaag catacccgtt tagcagcgga cttagccggg
    43981 ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta tggcctgcat
    44041 acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt tgacgttacg
    44101 gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga caacttcctc
    44161 ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc ggatgtcaca
    44221 aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga agcggcaaaa
    44281 tgtagcaatc aatcgccaat gcacattcgc tggtagtacc aggatgaaac gcgggaatgc
    44341 gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg
    44401 ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga cagtctggtg
    44461 ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc
    44521 aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc agcaatccag
    44581 gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga tatttgccct
    44641 tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc gccgattatt
    44701 gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa
    44761 caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa aaacaacatt
    44821 ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc gattgatatt
    44881 cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga agacggcagc
    44941 gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca gattgaagag
    45001 agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat caatcgcacc
    45061 tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt
    45121 ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat
    45181 gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc acggctgccg
    45241 gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac
    45301 atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat tttgataatg
    45361 ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt ttcaggtgtt
    45421 ggctcatcga catcttcatc ctccggctcg agttttactt tcgtcaacag catccgggtg
    45481 ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag acgggccagc
    45541 atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata caaatccagt
    45601 aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc ccatgctttg
    45661 cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt tccgctatgt
    45721 aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata tttcagcggt
    45781 tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa cgcgaaataa
    45841 tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt agatgggtaa
    45901 ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca gtacaaacgt
    45961 tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg ccactgtttg
    46021 gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg caacagtttg
    46081 cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa aaacgcagtg
    46141 acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc aacagtctgg
    46201 ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc accagcctga
    46261 atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc attaaacgaa
    46321 tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata tcgcgcttct
    46381 atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt ccaggtattt
    46441 aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc tgaatccagt
    46501 aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc ttcatcatct
    46561 gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg gcgtaatgct
    46621 ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag ataagcctgg
    46681 cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca tgttttcata
    46741 ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat ggctgccagg
    46801 gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg
    46861 cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct tcgcactgag
    46921 aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc cagctgtgat
    46981 agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct tcttccgtca
    47041 gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa tcccaggctt
    47101 tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta taaatcaacc
    47161 ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag cgttgccatg
    47221 tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga taaatagtct
    47281 gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat tcttttatcc
    47341 cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt atttccatgg
    47401 gcgcagaaag cgcaaaaaga agatattgtt cttcattatt cattttttaa taatccctta
    47461 agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc tttcaaaata
    47521 aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt cgcaattttt
    47581 atagttgcag caattacctg tattagccaa cccaagaaaa cgatgttgcg tgataaagcc
    47641 atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt aaccacggca
    47701 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
    47761 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
    47821 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tgaggtggat ctacctgaaa
    47881 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
    47941 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
    48001 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
    48061 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
    48121 atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
    48181 gcccagacag cccagcccgc catcatcaac gggcattggc tgtggttgcg gctccggctc
    48241 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
    48301 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
    48361 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
    48421 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct tcgtgcaaaa ccatctcgac
    48481 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
    48541 cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt taaccagatc
    48601 acgcgcttca atcatcatca ccactaatgg gtaataatag acccgatgct gacggcacca
    48661 ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt aacaaagttc
    48721 tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat aataacgcgc
    48781 atcttcattg tcaggttcag cttcccgcca cgaccgggcg taatccagga gttcggggat
    48841 tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
    48901 gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
    48961 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa ctggcagatg
    49021 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
    49081 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
    49141 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
    49201 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
    49261 ggcggaggac agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat
    49321 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
    49381 aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
    49441 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
    49501 ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc cttaagcgtt
    49561 tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta atacgcatca
    49621 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
    49681 aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata ttgccgacca
    49741 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
    49801 atacgcgact ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
    49861 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
    49921 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga
    49981 tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc tgccaaccga
    50041 gcggcatgac cgttctcgat cattcggtga attatgcgca gcaggtcatc acggtcatca
    50101 attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggggatgaa
    50161 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
    50221 ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga atgtaaacta
    50281 catttcgagg ctcagttatt tatacttttc tgacgcaaca taatacgcat aaaataaggc
    50341 ttacgcaaaa atggaataca taaaaatata atatatttta tctacaacca taaggaaatg
    50401 ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
    50461 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
    50521 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
    50581 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
    50641 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
    50701 gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg
    50761 gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttgga
    50821 tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt tttctggtac
    50881 aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga tctttataaa
    50941 gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
    51001 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
    51061 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
    51121 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
    51181 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
    51241 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
    51301 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
    51361 gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat tctgcccata
    51421 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
    51481 gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
    51541 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
    51601 atgatgattg aggtcctgga aaatttcgcg ccattcaagc tgcgttttat cggtgatgtc
    51661 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
    51721 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
    51781 cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
    51841 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca gtgacgcgac
    51901 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
    51961 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
    52021 taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
    52081 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
    52141 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
    52201 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
    52261 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
    52321 cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat gctgggcaaa
    52381 aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
    52441 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
    52501 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
    52561 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataaa
    52621 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
    52681 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
    52741 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
    52801 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
    52861 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
    52921 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
    52981 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
    53041 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
    53101 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
    53161 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
    53221 tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc agatctttct
    53281 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac
    53341 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
    53401 aaagtgaact accgcctgcg cgactggggt gtttctcgcc agcgttactg gggcgcgccg
    53461 attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga ccagctgccg
    53521 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
    53581 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
    53641 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
    53701 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
    53761 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
    53821 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
    53881 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
    53941 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
    54001 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
    54061 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
    54121 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
    54181 cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
    54241 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac tatcgctaaa
    54301 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
    54361 ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc tctgatgcag
    54421 gaagcactgc aggccgttgt ccgtatgctc aacccgttca tcccgcacat ctgcttcacg
    54481 ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
    54541 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
    54601 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
    54661 gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat ttacgtacca
    54721 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
    54781 cactgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgaca
    54841 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
    54901 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
    54961 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
    55021 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
    55081 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcat
    55141 tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
    55201 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
    55261 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
    55321 gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
    55381 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
    55441 ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag aacaccacac
    55501 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
    55561 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
    55621 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tattgatcgt
    55681 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
    55741 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
    55801 tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg tgctctgcta
    55861 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
    55921 gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
    55981 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
    56041 tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg
    56101 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
    56161 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
    56221 cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
    56281 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
    56341 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
    56401 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
    56461 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
    56521 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
    56581 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
    56641 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
    56701 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
    56761 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
    56821 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
    56881 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
    56941 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact tacattaacc
    57001 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
    57061 tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
    57121 cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag atgatgtttc
    57181 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
    57241 cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggcga
    57301 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
    57361 gtgcaatgac tggcagcatg ctatccccac gaatggtgaa ggatttcagg cattttcgca
    57421 acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga atattttaat
    57481 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca tgcctgccga
    57541 gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
    57601 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaacg catgactttt
    57661 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
    57721 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    57781 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    57841 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    57901 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    57961 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    58021 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    58081 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    58141 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    58201 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    58261 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    58321 aagatatgcc cttcgagctg attgaaattc cggccggcaa acgcggcaag aatgcggaca
    58381 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    58441 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    58501 aacgctggaa gctggatggt cgcgacgtaa gtctactgat tggcgggcct gaagggttgt
    58561 cgcctgcctg taaagcggcg gctgagcaga gctggtcgct gtcggcgctt accctccccc
    58621 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    58681 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    58741 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    58801 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    58861 aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt aagctggtgc
    58921 ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg gccctcaacc
    58981 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
    59041 acgctttgcg cagcgtggta gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    59101 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctaacc gaagtacaag
    59161 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    59221 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    59281 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    59341 atgatattgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    59401 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    59461 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    59521 aaacgctgct ggcgggtagc cgcgcagctg tggttgttac cgaaccgcgt acaggtgggg
    59581 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    59641 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
    59701 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattaagcg
    59761 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctaccagggt
    59821 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    59881 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    59941 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    60001 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    60061 ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca gcgaccccaa
    60121 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
    60181 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
    60241 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    60301 ctaaccgccc taatggtacg gcgaataaat actttgccag cgcaccgtac aaaattgcgg
    60361 cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    60421 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    60481 cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg gttggtacgc
    60541 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    60601 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    60661 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgattttac tggcattgct
    60721 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    60781 taaaatcggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    60841 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    60901 ggtagatgct ttcggagcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    60961 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    61021 caaccgcgac gtttgcccgc catcgttgaa gaatactggc atcgcgctgg tgctgatatt
    61081 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    61141 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    61201 agtgctggta gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    61261 ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg gctatcacat
    61321 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    61381 cactcagtca cagcttgaat ttctccccga acgccatact gactttatct tcgcggtact
    61441 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    61501 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    61561 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    61621 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    61681 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    61741 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    61801 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    61861 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    61921 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    61981 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    62041 tgacggcctc tggcgaagct ttcgatccga caaagctgac ggcggcccat ccaacgcttc
    62101 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    62161 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc gctttctcgc gcggcagctg
    62221 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    62281 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    62341 tgcctgcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    62401 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    62461 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctagtgta ctggaaggca
    62521 gcgaaccgac gcctgcgcca cagcccgttg ttacagcttc gtcgacaacg cctgcaacct
    62581 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    62641 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    62701 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    62761 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    62821 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    62881 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    62941 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    63001 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    63061 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    63121 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    63181 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    63241 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    63301 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    63361 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    63421 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    63481 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    63541 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    63601 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    63661 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    63721 acactgacaa agcaggttac aatcttgttg cttctgcgac tgaaggccag atgcgcttga
    63781 tttctgcggt gatgggtgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    63841 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    63901 cctctgaacc ggtttggttt agtgattctg atcgcgcttc gttaggggtt gataaagacg
    63961 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    64021 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    64081 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    64141 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    64201 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc
    64261 ccgccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa
    64321 acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt
    64381 acctgagctg gttgatcagg tggttgaagt ggtacagcgc catgcgccag gtgactacac
    64441 cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc
    64501 cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg
    64561 catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact cccctctcct
    64621 cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc
    64681 aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccgggcta
    64741 tgcatgaatt caccgatacc cgcgataata gtacccttga tgaaatctgg ctggtcgagc
    64801 actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg
    64861 atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccgggacaac
    64921 aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga
    64981 ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc
    65041 gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg ggtttacgaa
    65101 ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat
    65161 ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga
    65221 aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac
    65281 taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg gcccaattta
    65341 cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt
    65401 ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac cccattgatt
    65461 aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt agacataaga
    65521 ttattgtaat tttttaaata ttattttccc atgaataaaa ttggagtaag tgcgtggata
    65581 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    65641 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    65701 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    65761 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    65821 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    65881 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    65941 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    66001 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    66061 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    66121 ttctgttcac cgacaatatg gtgttaattt gccgcaaggg aaatccacta ctctctttag
    66181 aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg gaagggcaaa
    66241 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    66301 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    66361 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    66421 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc atcctgcgtg
    66481 atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    66541 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    66601 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    66661 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    66721 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    66781 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    66841 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    66901 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    66961 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    67021 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    67081 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    67141 gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg tattactgcc
    67201 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcga
    67261 atggatcgtg ctctggatat tctgactgca acgcccccag atgtgttcaa ccataacctg
    67321 gaaaacgtac cgcgtattta ccggcaggta cggcctggcg cagattacaa ctggtcgctg
    67381 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    67441 gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    67501 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    67561 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    67621 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    67681 aaaggaatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    67741 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    67801 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    67861 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    67921 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca
    67981 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    68041 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    68101 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    68161 acggttgttt aagatgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    68221 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    68281 aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    68341 ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg
    68401 aacccaggcg gcaggaagta ccaggatttc agctccctgt aaagcctgtg ccagcgccag
    68461 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag
    68521 taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc
    68581 atagagatgc agtttggcat aacgggcgac gatgtttcct gcctgaagtg ccaccagcat
    68641 attccatgcg cgccccggcg ttgaaggaag atgaatcgtc agaattgtcg tcatcatgtt
    68701 acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    68761 aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacaaacag
    68821 cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    68881 ccacacagat gtaacagcaa actgtcctgc agcaaccagc attgccacct cctgtcagag
    68941 tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat
    69001 agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa
    69061 gcgtagcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcg attccgttgg
    69121 ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca
    69181 gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg
    69241 gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct
    69301 ttggctgggc attactgaac gttttcgtca accttctggg gtcttttgcc atgaccgcac
    69361 tggcattctg gctgttttcg gcctcaaccg tacactaaag accaaaaaaa acccgctgat
    69421 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    69481 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    69541 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    69601 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    69661 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc agtacaccaa
    69721 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    69781 gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    69841 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    69901 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    69961 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    70021 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    70081 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    70141 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    70201 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    70261 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc tcatctgcgt
    70321 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    70381 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    70441 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    70501 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    70561 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat gacaaaatgc
    70621 aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt aacgaagtat
    70681 ataatatata tcgtcaacca tgctcatatt agattatttt ttgagcgcac agccgcagct
    70741 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    70801 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    70861 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    70921 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    70981 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtggctcgc
    71041 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    71101 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    71161 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    71221 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    71281 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    71341 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    71401 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    71461 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    71521 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    71581 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    71641 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    71701 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    71761 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    71821 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    71881 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    71941 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    72001 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    72061 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    72121 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    72181 gcgtcaaacc tggatcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    72241 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    72301 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    72361 gacaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    72421 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    72481 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    72541 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    72601 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    72661 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    72721 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    72781 ccgggagaac attggattat tcgggatctg atgggattag atttggtggg gcttgcaagc
    72841 ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt
    72901 tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca tagcttcaaa
    72961 aaacgttcaa aaccgataac aaccaggctg tcaccaaatg actcatatca caaatcagct
    73021 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggct
    73081 catggttgtt atacggcttt tttcgcatct tgtggttcca acaaaggagc tacaggctct
    73141 tcctgtttat gaacaccata cattgacaaa atctcttggc gagtttctgg aggtaattta
    73201 tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac ccagcgttgc caatagggtg
    73261 tcaacggaga tggaggggcg ttcttgcatt aatccggaaa gcccatattt agccatgttt
    73321 agctctatct ctcggacttc attttctcct agtttattga ttaacaggga gaacattttc
    73381 aatgtatcag cattcatttc ttcgatagca attttttgca tagccggtat tgccgggata
    73441 tattcaaccc caggcccgcg acgcccttct tgtgaacgct tcggccatcc ttctttcttt
    73501 gctcgtttat gcaccccctg tattgtccca ggcattccat ccagttcagt aatttgtttg
    73561 ggtgtcatcc actggtttat ctcatttgcg cttttcataa actaagttta ccagatttat
    73621 ctatataaaa atcaatggat tacaaaataa accacattaa ctgatataaa ccggttttgt
    73681 ttatttggta atgtataaaa cagataatca agacgaatta tttgttttgg taacccttta
    73741 tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga ttggcatcgg gccgacgtga
    73801 ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt gtccagacgt tcaggttatt
    73861 caaccgggac gcttgcaaat gcattagtca ggccatggcc taaaggtgaa cgcattattg
    73921 ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag tagatatcta agggatcaca
    73981 atgagagagt ggatatcagc aattgaatgc atgggtttat ctggtttacc tggttcatta
    74041 atgggggttc acaaaagagc tcagagagaa ggatggctca agagaagaag agaaggaatt
    74101 agaggggttg cttacgaata ccatatatct tctcttccta acattgctcg agctgaagtt
    74161 ttgctccaac aaggaaaatt taaaaccagt cagggatgtt ttgaaatcgc ccgccccacg
    74221 ctggaagctc atgattatga tcgtgaggca ctatggagca aatgggataa agccagcgat
    74281 tcccagcgca gcctggctga aaaatggttg ccctcaattc aggccacaga cgaaatgctg
    74341 aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta ccaggtcagc
    74401 gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc tgactgggcg
    74461 gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg ttcacaaaag tgaatttgac
    74521 gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc cgccttccgc
    74581 aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat tccctcccgt
    74641 gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc ctgtcgtgaa
    74701 ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga acacctggac
    74761 gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg ctggtttaac
    74821 ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg aaaaattctg
    74881 ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt catggatgtt
    74941 gtgactcgct acagtatccc ggaggatttt cacatcacca ttgataacac ccgtggtgct
    75001 gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt aaaagaggac
    75061 gatccaaaag gactgttttt actgatgggt gcgaaaatgc actggacaag cgttgttgcc
    75121 ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg tgggcttgag
    75181 gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa tccgcaggca
    75241 aaacctgata actatggcga ccacgctgtt gatgcagagc tgtttctgaa aacctttgcc
    75301 gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg cgggggcaaa
    75361 ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg taagccaacc
    75421 gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc acgcaaaggc
    75481 gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta ttacaacatg
    75541 gcattaatga atgccggagt gaaaaaagtt gtggtcagat ttgatccaca gcagttacac
    75601 agcacggttt a
//
LOCUS       DAJTNW010000006        71815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNW010000006 DAJTNW010000000
VERSION     DAJTNW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(1342..2937)
                     /locus_tag="OO179_000413"
     CDS             complement(1342..2937)
                     /locus_tag="OO179_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5530602.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3292..5463)
                     /gene="glcB"
                     /locus_tag="OO179_000414"
     CDS             complement(3292..5463)
                     /gene="glcB"
                     /locus_tag="OO179_000414"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5530603.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5485..5889)
                     /locus_tag="OO179_000415"
     CDS             complement(5485..5889)
                     /locus_tag="OO179_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5530604.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5894..7113)
                     /gene="glcF"
                     /locus_tag="OO179_000416"
                     /pseudo
     CDS             complement(5894..7113)
                     /gene="glcF"
                     /locus_tag="OO179_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7124..8176)
                     /gene="glcE"
                     /locus_tag="OO179_000417"
     CDS             complement(7124..8176)
                     /gene="glcE"
                     /locus_tag="OO179_000417"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5530605.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8176..9675)
                     /gene="glcD"
                     /locus_tag="OO179_000418"
                     /pseudo
     CDS             complement(8176..9675)
                     /gene="glcD"
                     /locus_tag="OO179_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9926..10710
                     /gene="glcC"
                     /locus_tag="OO179_000419"
                     /pseudo
     CDS             9926..10710
                     /gene="glcC"
                     /locus_tag="OO179_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10834..11975)
                     /locus_tag="OO179_000420"
                     /pseudo
     CDS             complement(10834..11975)
                     /locus_tag="OO179_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12337..14067
                     /locus_tag="OO179_000421"
     CDS             12337..14067
                     /locus_tag="OO179_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5530606.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14064..14978
                     /locus_tag="OO179_000422"
     CDS             14064..14978
                     /locus_tag="OO179_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5530607.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15011..15259
                     /locus_tag="OO179_000423"
     CDS             15011..15259
                     /locus_tag="OO179_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5530608.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15259..16430
                     /locus_tag="OO179_000424"
                     /pseudo
     CDS             15259..16430
                     /locus_tag="OO179_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16466..17545)
                     /gene="lptG"
                     /locus_tag="OO179_000425"
     CDS             complement(16466..17545)
                     /gene="lptG"
                     /locus_tag="OO179_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5530609.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17542..18612)
                     /gene="lptF"
                     /locus_tag="OO179_000426"
     CDS             complement(17542..18612)
                     /gene="lptF"
                     /locus_tag="OO179_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5530610.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18643..19203)
                     /locus_tag="OO179_000427"
     CDS             complement(18643..19203)
                     /locus_tag="OO179_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5530611.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19215..20039)
                     /locus_tag="OO179_000428"
     CDS             complement(19215..20039)
                     /locus_tag="OO179_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530612.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20039..21388)
                     /locus_tag="OO179_000429"
     CDS             complement(20039..21388)
                     /locus_tag="OO179_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5530613.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21434..22192)
                     /locus_tag="OO179_000430"
     CDS             complement(21434..22192)
                     /locus_tag="OO179_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530614.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22224..22937)
                     /locus_tag="OO179_000431"
     CDS             complement(22224..22937)
                     /locus_tag="OO179_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5530615.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23111..23803
                     /locus_tag="OO179_000432"
     CDS             23111..23803
                     /locus_tag="OO179_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5530616.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23852..25351)
                     /gene="pitB"
                     /locus_tag="OO179_000433"
     CDS             complement(23852..25351)
                     /gene="pitB"
                     /locus_tag="OO179_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5530617.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25643..27502)
                     /gene="gss"
                     /locus_tag="OO179_000434"
     CDS             complement(25643..27502)
                     /gene="gss"
                     /locus_tag="OO179_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5530618.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27707..28573
                     /gene="yghU"
                     /locus_tag="OO179_000435"
     CDS             27707..28573
                     /gene="yghU"
                     /locus_tag="OO179_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5530619.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28696..28944)
                     /gene="hybG"
                     /locus_tag="OO179_000436"
     CDS             complement(28696..28944)
                     /gene="hybG"
                     /locus_tag="OO179_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5530620.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29078..29419)
                     /gene="hypA"
                     /locus_tag="OO179_000437"
     CDS             complement(29078..29419)
                     /gene="hypA"
                     /locus_tag="OO179_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5530621.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29412..29900)
                     /gene="hybE"
                     /locus_tag="OO179_000438"
     CDS             complement(29412..29900)
                     /gene="hybE"
                     /locus_tag="OO179_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5530622.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29893..30387)
                     /locus_tag="OO179_000439"
     CDS             complement(29893..30387)
                     /locus_tag="OO179_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5530623.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30387..32090)
                     /gene="hybC"
                     /locus_tag="OO179_000440"
     CDS             complement(30387..32090)
                     /gene="hybC"
                     /locus_tag="OO179_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5530624.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32087..33265)
                     /gene="hybB"
                     /locus_tag="OO179_000441"
     CDS             complement(32087..33265)
                     /gene="hybB"
                     /locus_tag="OO179_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5530625.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33255..34241)
                     /gene="hybA"
                     /locus_tag="OO179_000442"
     CDS             complement(33255..34241)
                     /gene="hybA"
                     /locus_tag="OO179_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5530626.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34244..35362)
                     /gene="hybO"
                     /locus_tag="OO179_000443"
     CDS             complement(34244..35362)
                     /gene="hybO"
                     /locus_tag="OO179_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5530627.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35551..35838)
                     /locus_tag="OO179_000444"
     CDS             complement(35551..35838)
                     /locus_tag="OO179_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5530628.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35957..36844)
                     /locus_tag="OO179_000445"
     CDS             complement(35957..36844)
                     /locus_tag="OO179_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5530629.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37001..38041
                     /gene="gpr"
                     /locus_tag="OO179_000446"
     CDS             37001..38041
                     /gene="gpr"
                     /locus_tag="OO179_000446"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5530630.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38081..38575)
                     /locus_tag="OO179_000447"
     CDS             complement(38081..38575)
                     /locus_tag="OO179_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5530631.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38766..39650
                     /gene="yghA"
                     /locus_tag="OO179_000448"
     CDS             38766..39650
                     /gene="yghA"
                     /locus_tag="OO179_000448"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5530632.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39922..40347)
                     /gene="exbD"
                     /locus_tag="OO179_000449"
     CDS             complement(39922..40347)
                     /gene="exbD"
                     /locus_tag="OO179_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5530633.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40354..41088)
                     /gene="exbB"
                     /locus_tag="OO179_000450"
     CDS             complement(40354..41088)
                     /gene="exbB"
                     /locus_tag="OO179_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5530634.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41340..42527
                     /gene="metC"
                     /locus_tag="OO179_000451"
     CDS             41340..42527
                     /gene="metC"
                     /locus_tag="OO179_000451"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5530635.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPKGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42667..43326
                     /locus_tag="OO179_000452"
     CDS             42667..43326
                     /locus_tag="OO179_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5530636.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43366..44265)
                     /gene="yqhC"
                     /locus_tag="OO179_000453"
     CDS             complement(43366..44265)
                     /gene="yqhC"
                     /locus_tag="OO179_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5530637.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44459..45622
                     /gene="yqhD"
                     /locus_tag="OO179_000454"
     CDS             44459..45622
                     /gene="yqhD"
                     /locus_tag="OO179_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5530638.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45727..46554
                     /gene="dkgA"
                     /locus_tag="OO179_000455"
     CDS             45727..46554
                     /gene="dkgA"
                     /locus_tag="OO179_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5530639.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46754..47680
                     /locus_tag="OO179_000456"
     CDS             46754..47680
                     /locus_tag="OO179_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5530640.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47731..47988
                     /gene="yqhH"
                     /locus_tag="OO179_000457"
     CDS             47731..47988
                     /gene="yqhH"
                     /locus_tag="OO179_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5530641.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48031..50250)
                     /locus_tag="OO179_000458"
     CDS             complement(48031..50250)
                     /locus_tag="OO179_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5530642.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50361..51773)
                     /gene="ftsP"
                     /locus_tag="OO179_000459"
     CDS             complement(50361..51773)
                     /gene="ftsP"
                     /locus_tag="OO179_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5530643.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51848..52585)
                     /gene="plsC"
                     /locus_tag="OO179_000460"
                     /gene_synonym="parF"
     CDS             complement(51848..52585)
                     /gene="plsC"
                     /locus_tag="OO179_000460"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5530644.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52819..55077)
                     /gene="parC"
                     /locus_tag="OO179_000461"
     CDS             complement(52819..55077)
                     /gene="parC"
                     /locus_tag="OO179_000461"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5530645.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55623..56105)
                     /locus_tag="OO179_000462"
     CDS             complement(55623..56105)
                     /locus_tag="OO179_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5530646.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56158..56550)
                     /gene="ygiW"
                     /locus_tag="OO179_000463"
     CDS             complement(56158..56550)
                     /gene="ygiW"
                     /locus_tag="OO179_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5530647.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56702..57361
                     /gene="qseB"
                     /locus_tag="OO179_000464"
     CDS             56702..57361
                     /gene="qseB"
                     /locus_tag="OO179_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5530648.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57358..58632
                     /gene="qseC"
                     /locus_tag="OO179_000465"
     CDS             57358..58632
                     /gene="qseC"
                     /locus_tag="OO179_000465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5530649.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58678..59010)
                     /locus_tag="OO179_000466"
     CDS             complement(58678..59010)
                     /locus_tag="OO179_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5530650.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59329..59910
                     /gene="mdaB"
                     /locus_tag="OO179_000467"
     CDS             59329..59910
                     /gene="mdaB"
                     /locus_tag="OO179_000467"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5530651.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59941..60255
                     /locus_tag="OO179_000468"
     CDS             59941..60255
                     /locus_tag="OO179_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5530652.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60303..62195)
                     /gene="parE"
                     /locus_tag="OO179_000469"
     CDS             complement(60303..62195)
                     /gene="parE"
                     /locus_tag="OO179_000469"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5530653.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62224..62805)
                     /gene="yqiA"
                     /locus_tag="OO179_000470"
     CDS             complement(62224..62805)
                     /gene="yqiA"
                     /locus_tag="OO179_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5530654.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62805..63632)
                     /gene="cpdA"
                     /locus_tag="OO179_000471"
     CDS             complement(62805..63632)
                     /gene="cpdA"
                     /locus_tag="OO179_000471"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5530655.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63657..64079)
                     /locus_tag="OO179_000472"
     CDS             complement(63657..64079)
                     /locus_tag="OO179_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5530656.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64080..64709)
                     /gene="nudF"
                     /locus_tag="OO179_000473"
     CDS             complement(64080..64709)
                     /gene="nudF"
                     /locus_tag="OO179_000473"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5530657.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64914..66395
                     /gene="tolC"
                     /locus_tag="OO179_000474"
     CDS             64914..66395
                     /gene="tolC"
                     /locus_tag="OO179_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5530658.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66543..67214
                     /locus_tag="OO179_000475"
     CDS             66543..67214
                     /locus_tag="OO179_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5530659.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67220..68380
                     /locus_tag="OO179_000476"
     CDS             67220..68380
                     /locus_tag="OO179_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5530660.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68418..69206)
                     /gene="ygiD"
                     /locus_tag="OO179_000477"
     CDS             complement(68418..69206)
                     /gene="ygiD"
                     /locus_tag="OO179_000477"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5530661.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69349..70122
                     /gene="zupT"
                     /locus_tag="OO179_000478"
     CDS             69349..70122
                     /gene="zupT"
                     /locus_tag="OO179_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5530662.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70180..70350)
                     /locus_tag="OO179_000479"
     CDS             complement(70180..70350)
                     /locus_tag="OO179_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530663.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70611..71264)
                     /gene="ribB"
                     /locus_tag="OO179_000480"
     CDS             complement(70611..71264)
                     /gene="ribB"
                     /locus_tag="OO179_000480"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5530664.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71368..71516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71638..>71815
                     /gene="ubiK"
                     /locus_tag="OO179_000481"
     CDS             71638..>71815
                     /gene="ubiK"
                     /locus_tag="OO179_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5530665.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQAQVL"
BASE COUNT        17937 a        19113 c        17707 g        17058 t
ORIGIN      
        1 acgtaccgtt taaacaaaga caatacgtct ctttatttct cataaaaccc caatccctta
       61 cagggcttat tgaaaaattt aaaaatcaca aatttctcaa tactcgaaat gagatcacat
      121 aagaaatcaa aaagccttct catctatttt aagttttccc aacggaaaat aagagctttc
      181 attaatactg aaccacttta ctgttctatt tgttcaggta ttgcattacg gtgtgctgag
      241 atccatcggt gcatccctgt atcccgcgat gagactaaaa tagtcgtgat gtaagctcgt
      301 atcagattct tagtgaatac cacatttgtt ataattaagt gaaaagatac attgctgatt
      361 tacattattt ggtttacaaa atgtcatcac ttgcagagtt atcgctgaca gataccttta
      421 atctcttact actttcgtgt tattgcaata caaccatccg taaagcaggg ctatttttag
      481 caggaaatac tcaggtgtca acattcgaac tccaactctg aaatattcat ataagttaat
      541 gattttaatg attttaatga ttttaatgat tttaatgatt ttaatgattt tttcatttat
      601 tcacacctaa taaaagtatt caaattcaga tgatttttct gctcaacaga cacaaacagg
      661 caataatact ctccaaagcg aacaatattt ggcattacga tttaatgctt cataatcacc
      721 aaattattta accataaatt ttcttatttc taacaaaatg attggtgtgt tgttggagtt
      781 aaaatacata cataatatta acatctgcca atatttcact ttccgcaaga cggatgattt
      841 cgaatcctca tccacgttac cctatcccaa atatctttat taataggcag taacgaacac
      901 tgttatagca agattaactt attaacctaa cggcatcttt tatgtaacaa gtggagataa
      961 attgcttaaa attgatcctc gacaaatctt aaaagttctc attattctgc aaaatcagct
     1021 ttgcgaataa tcttatgttt ttgggagttt tgggaatttt gatctactca ttgttctagt
     1081 gaaaaatata aataagagaa aaaacgccat tcgtcatgaa agcaacagat gaagcaatgt
     1141 aataacatac taaaattgat ctcatgaaag tgggtacttt ataaccagca cagaagccaa
     1201 gttgtataaa tattaaacaa ttcgcatcgt aaaacggaga ctacgtataa agaagccaat
     1261 cgtaaaaacg gttccctggc gagataaacg acagacaaat ggaaagccgg gtgatattta
     1321 ccttcatccg gctttattga tttacgagac taacatcccg gtaaacacat acgcctgcag
     1381 cagggtgata acgccgataa cgctggcaaa aatcagactg tgcttcacgg tgtagcggaa
     1441 cagttcagat tctcggccca ccatgcccgt cgcggcgcag gccacggcga tagattgcgg
     1501 ggagatcatc ttgccagtta cgccgccgct ggtatttgct gccaccagca gagtgtcaga
     1561 gacgttgatt tgctgcgccg tggtcgattg cagtgaacca aacagggcgt ttgaagaagt
     1621 gtccgacccg gtaaggaata cgcccagcca gccgagaaac ggtgagaaaa acgggaacat
     1681 cacgcctgta cctgccagta ccagcgccag cgtggtggac atgccggaat agttggtgac
     1741 gaaggcgaat gccagcacca tgccaatcga caatatcggc cacttcagac taattagcat
     1801 ttcggcaaag acgccaatcc ctttcttgat gcccacgccg aggatgaaga tagagataat
     1861 cgccgcaata aaaatagcgg tgccgccagc cgagagaggg tcgaatttaa acaccgcatc
     1921 cattggcgtt ggttgggcga caatgggtgc cgctttcaac acttgttgat gcaaatgagg
     1981 gatctggaaa ttaatcacca gtgaataaaa cgcgccgccc ggagcaaata acgctttaaa
     2041 cggcttcatg gtccagatgg tgaccagcac cgttaagatt aaaaacggtg accacgcacg
     2101 aatgatttgc cccagactat attctgaagg cacgggaccg ccagaagatg gcttatttac
     2161 caccatcgca cctgcggatt gccccatgct gattgccgtt tcggtatttt tcggccgcca
     2221 gactttaaga aataaagcga gtgagacgat actcaccagc gccgaagtaa tatccggcag
     2281 ttccggacca atatagttag aggtaaagaa ctgagtgaca gcgaagcttc ccccagcaac
     2341 cagcgccgct ggccacgtct ctttcacccc tttccagccg tccatcattg ctaccagcca
     2401 gaacggcaca agaaccgaca ggaacggtaa ctgacgtccc gccattgcgc caatgtggaa
     2461 cggatcgatt cccgttacct gaccggcgac cagaatcggc acgcccaacg caccaaacgc
     2521 caccgtcgca ccaaagccag cagctccttc cagcaacgca ccaaaggaga aaccaatcag
     2581 taacacctgc aaacgctgat cgtcggtgat ggagataacc aagctgcgga taatatcgaa
     2641 ctgcccgctg gcaacggtta atttatacag gaacaccgcc gcgacaataa tccacgcaat
     2701 tggccataat ccataaataa agccatagcc cgcagcagca aatgccatat caatcggcat
     2761 tttaaaggcg aatatcgcaa tcaggataga taatataagg gttattgctc cagcgacatg
     2821 tcctttcaga cgtaataccg cgagtgcaac gaagaagaat attatcggga tcagggcgac
     2881 cagagcggat agccccagtc ctcccatcgg catatacatt tgggtccagg taaccatatt
     2941 gttcagtctc ttattatctt tatatgcttg atatacttaa ggttgtaata agcaaaagag
     3001 gactgaactg taaaatatag gcgttatact ttacagcaac agtacgccgc taacgcaatt
     3061 gctacctctg gcataacaag tatatcgggt aagggtttct gttccgcaca cgcaggcgca
     3121 gagtatcgtt aagatgtcca tattgttgtt ttaggcccgc tagtaatgcg ctacgggtat
     3181 ttaatattgt taaaccctga taatcgctcc ggttatttcc gggataaatg tactaccgca
     3241 gttactatca tagccccgac aataaaactt gccggggctt ttttgacgct attaatgact
     3301 ttctttttcg cgtaaacgcc aggcgtgtaa taacggttcg gtataaccgt ttggctgttt
     3361 cacgccgagg aagattaaat cgctggcagc tttaaaagca cacgagttag cgaaattccc
     3421 cgccatcgga cgataagccg gatcgccagc attttgctga tcaaccactt tcgccatatt
     3481 ctccagcgac gcctgcacct gttctttggt cagaataccg tgacgtaacc agttggcgat
     3541 atgctggctg gagatacgca gcgttgcgcg gtcttccatc agcgccacgt tgtgaatatc
     3601 cggcactttt gaacaaccaa tcccctgctc cacccaacgc accacgtacc ccagaatccc
     3661 ctgcacgttg ttatccagct cttgttggat ctcttgcgcc gaccagttag cgttttcagc
     3721 caccggaata gtcagcagat cgtccagcag cggttcaaat tcagcactga actcggtctg
     3781 ggcaatgttg gcttgcacgc tctgtacgtt ggtatggtgg tagtgcagcg catggagcgt
     3841 agcagcggtt ggtgacggaa cccaggctgt gtttgccccg gcacgcagtt ggtcgccctt
     3901 ctggctgtac atgtctgcca tcaggtccgg cattgcccac atgcctttac cgatttgcgc
     3961 tttaccgcgc agcccacaga acagaccgga aagcacgtta ttacgttcgt aggctttgat
     4021 ccacggcgtc gatttcatct ggtttttacg cagcatcggg ccagcttcca tcaccgaatg
     4081 catttcatcg ccggtacggt cgaggaaacc ggtattgatg aacgccacgc ggttgcgcgc
     4141 ctgagcgata cagctacgca agttcagcga ggtccggcgt tcttcatcca taatgcccat
     4201 tttcagggta ttcggtgcca taccgagcat tgtctcaatg cgggtaaaca gtttgttggc
     4261 gaacgccact tcctgcggac cgtgcatttt cggtttcaca atatagacgc tgccagtgcg
     4321 cgagtttttc tgcactttta aatcatagag ggcaatcgcg ccagtcatga cgccatcaag
     4381 aataccttcc gggatttcat tgccttcgct gtcccaaatc acaggaatgg tcatcaaatg
     4441 acccacgttg cggataaaca gcaacgagcg tccgtgcaga gaaatttcag agccatcggc
     4501 ggcggtgtac tgacgatcgt cattcagttt acgtacgatt tgccgaccgt ttttctccat
     4561 tttctcttgc agagtcccct gcatcaggcc cagcagattg cggtacagca ggattttatc
     4621 ttccgcatca accgccgcga ccgaatcttc gcagtcgaga atggtactga tagcagcttc
     4681 gacgataaca tcgttgatgt gcgccggatc gtctttgcca atccgcccgt tggcatcaat
     4741 ttgcagttca atatgcaggc cgttattttt cagcaaaatg caggtcggcg cagcggcatc
     4801 gccacggtaa ccgacaaact gtgctggagt acgtaacgtg gtttctttac catttttcaa
     4861 ctggatgcgt aattgtttat cgaccacctt aaacgccacc acatcctgat agctgccgtt
     4921 ttccagcggt agtgattcat cgaggaaacg tcgaacccag gcgataacct gctcaccgcg
     4981 ctgcggatcg tagccactga ccatcgcccc ttcctgcggg atgatgtcgc tgccgtataa
     5041 cgcatcgtac agtgagcccc agcgagcgtt cgccgcgttc agcgcgtagc gggcgttcat
     5101 tgccggaacc accagctgtg gccccgcctg actggtgatt tcgctgtcaa tgcccgtggt
     5161 ttccaccgtc acgcgctccg gttgcggcac caggtagccc agttcacgaa ggaaagattt
     5221 ataggccgct ttatctttta ccggccccgg attgctgcga tgccactcat caagcgccgc
     5281 ctgaatgcga tcgcgttctg ccagcaactg acgattttct ggtgccagat cgtgaacgat
     5341 ctcatcaaca ttgtgccaga atgcagcagc gtccagttcc acgcccggca aaacttctcc
     5401 atccacaaaa cgtttaaaat tggcgtcaat gcgtaaacgg ccctgggtta tggtttgact
     5461 cattgtttct ctcctcgttt tcgcttattt cgccaacacc gctgctgcca ctttcgcgac
     5521 ctgcgcatcc tgtgctccgg ttaaaccaga aacgcccacg gcaccaataa tttgcccatc
     5581 aacaacaacc ggtacgccgc cttccagcga cgtcagtaac ggcgcagtca cgaacgcggt
     5641 acgtccgttg ttcaccatct cttcatagcc cttagtttca cgacgcccca gcgcggcggt
     5701 acgcgctttc tcctgggaga tgtaagccgc aatcggcgcg caatcgtcca tgcgacttaa
     5761 cgccagcaga tgaccgccgt catcggcaac agcaatggaa acagaccagt tatttttctg
     5821 cgcctcttcc tgacctgcgg caataattgc actcgccatt tgctggctaa gaatgacttt
     5881 agttttcatt tttttattcc ttttcaaggg cttgttctac aatttcaatc cagtgacgca
     5941 cagaggtacg accggcgctc gccagatgcg tctggcaacc aatgttggcg gtgacgatca
     6001 tttccggttt gccgctttcc agcgcattca ttttgttatc ccgcagctgg cgtgccagat
     6061 cgggatgcgt taacgcatat gttcccgctg aaccgcagca cagatggctg tcgggaacgt
     6121 ccgttaaggt aaatccaaga cgaagtaaca ctttttccac ttcgccgttc agcttttgcg
     6181 catgttgtag ggtacacggg cagtggaagg ccagtttttt atcgccgcga attgccagtt
     6241 tttccagcgg ttcctcgcgc agaagttcga ctaaatcgac cgccagttca ctgacctgac
     6301 gcgctttatc ggcatataac gcatcgtttt tcagcatctg cccatactct ttgacgaacg
     6361 cgccgcaacc gctggcggtt tgcaaaattg cctcggcacc tgcttcaatc gcgggccacc
     6421 aggcatcaat attattgcgc gcccgtgcca accctttctc ctgcgcatta agatgatagt
     6481 ccaccgcgcc acaacagcct gcttcgttag ctgacatgac gctgatcccc agacgatcca
     6541 gcactcgcgc agttgccgcg ttggtgttgg gcgaaagcgt aggctgggcg cagccttcca
     6601 gcattaaaac ccgacgctta tggcgcagcg gcggacgcgg tttagctttc accgtttcag
     6661 caggcagttt tgctctgacc tgttccggta aaaacggtcg cagcaccagc cctacctgcg
     6721 tcagcgcacg gaagaccgcc ggacgcggca ctacctggcg caatccttcg cgcagcattc
     6781 gctccggcag tgggcgtttc actttctgct cgacaatatc acgcccgata tccagcaaat
     6841 tgtgatagcg cacaccagaa ggacaggtgg tttcacaatt gcggcaagtg aggcagcgat
     6901 cgagatgctc ctgtgtttta agcgtgactt cgttgccttc cagcacctgt ttaatcagat
     6961 agatgcgccc gcgcggcccg tccagttcat cgcccagaag ctgataggtt gggcaggttg
     7021 cggtacaaaa tccgcagtga acacaggcgc acaggatgct gtcggcttcc agcgcgcgtt
     7081 ctgccgcatc tcttcggtta atcgggtttg catagcctgc tcctcaaagt tccgcgtaca
     7141 tgcgaccggg gttaaacacg ccgcaagggt cgagctgctg tttaagctgc tggtgatagc
     7201 ggaataaagg agccgatagc ggggcaaagc caccatctcc ggcactaaag cgggtcgcat
     7261 gaccgccagc gttgcgggcg atgcgatgga tttgattgtc atcggctgtc gatttcagcc
     7321 agcgtaacgc cccgccccag tcgatcagtt gctcgccggg taaatccatc atcggcgcat
     7381 cgctgggtaa tgaaatgcgc cataaggtac ctggtaacga gaagaacggc agttgttgtt
     7441 cacgcaattg ctgccagaac tgacccgcaa cctcttcgcc acccagcagt tcacgcgctg
     7501 cttttaccga tccttcgccg ccctcaaggc ggatccacaa cgcattgtcg aagtaacata
     7561 agccactaat gggtaatggc tggagttgcc actcggcgat ttcactcatg gcttcttgca
     7621 ggctgatttc ccgacgcagg ctcagagagg cacgcggtcg cggtaacact ttcattgaga
     7681 tttcagtgag cacgccaaga caaccatagc ttccggccat taaccgtgag agatcgtatc
     7741 cggcaacgtt tttcatcact tcgccaccaa aacgcagatg ttttccagtg ccggtaatga
     7801 tgcgcgtgcc gaggacaaaa tcgcggaccg aaccgctcca cgggcgacgc ggccccgcca
     7861 gcccgcaggc gaccatcccg ccccaggtgg cttcttcacc ataatgcggc ggctcgcagg
     7921 gaagcatttg cccggcgctc tccagcgccg cttcaatcgc caccagcggc gttccggcac
     7981 gagcggttat caccagctcg gtcgggtcgt aattaacaat gccgcgatga caacgcacat
     8041 ccagcgtttg cccggtgact gggcgaccta aaaaggcttt gctattgctg ccctgaatca
     8101 ccagcggcgt tttatcgcta attgcctgat tcacctgctc cagcagcgcc tgactgtaat
     8161 cacactcgcg tagcatcaaa agcgctccag ttcaggaaaa ggtaaatgac cgtgatgcac
     8221 atgcatggca ccaaattcag cacagcggtg tagcgtggga atgtttttcc cagggttcag
     8281 caaaccatcg gggtcaaacg ccgccttgac cgcatggaag gtcgtgattt catcgctgtt
     8341 gaactgggcg cacatttgat tgattttttc tcgcccgatg ccatgttccc cactgatgct
     8401 gccgccaact tcaacgcaga gttcgaggat cttcccgccc agctcttccg cgcgggcaaa
     8461 ttcaccaggt tcgttggcat cgaaaaggat taacgggtgc atgttgccat ctccggcatg
     8521 aaagacgttg gcaacacgta aatcatattg ctgcgataaa cgggcaatgc cttccagtac
     8581 gccaggcagg gcgcgacgcg ggatggtgcc atccatgcag tagtaatccg gggagatacg
     8641 tcctaccgcc gggaacgcat ttttgcgacc ggcccagaaa cgtacgcgct ctgcttcgtc
     8701 ctgtgccaaa cggacgtcag tcgcgcccgc tttcaacaag atgtagttaa cccgctcgca
     8761 gtcttcctgt acgtcagact ccacgccgtc cagttcgcat aacaaaatcg cttcggcgtc
     8821 gacgggataa ccggcatgaa taaaatcttc cgccgcgcgg atcgacaggt tatccatcat
     8881 ctccagcccg ccggggataa tgccattggc gatgatgtca ccaaccgcaa gtccggcttt
     8941 ttctaccgag tcaaagctgg ctaacagaac ccgcgccacg ggcggcttcg gcagcagttt
     9001 taccgtcact tcggtggtca cgccgagcat accttccgat ccggtgaaca gcgccagcag
     9061 gtcaaaacca ggtgaatcca gcgcgtccga tcccagcgtc agcgcctcgc cgtccagcgt
     9121 ttgcacttca attttcagca ggttatgtac ggtcagacca tatttcaggc agtggacgcc
     9181 accggcattt tccgccacat tgccgccaat tgaacaggcg atttgtgagg aaggatccgg
     9241 cgcgtaatag agattatgcg gcgcaacggc ctaggagatc gccaggttac atacgcccgg
     9301 ctgcacgcgc gcgcggcgac caacggggtt aatgtcgagg ctctctttaa agcgcgccat
     9361 caccaacaac acaccttttt ccagcggcag cgcgccacca gaaagcccgg tgcctgcacc
     9421 acgggtcacc accggtacac gcaggcgatg gcagacagcc agaatcgctg tcacctgttc
     9481 catttgctta ggcagaacaa ccagtaatgg acgcgtgcga tacgcgctca acccgtcaca
     9541 ctcgtaagga atgatctcct catcggtatg caggatctca agtccaggga catgctcacg
     9601 cagtgccatc agtaccgatg tgcggtcgac atcgggtaaa gcgccatcaa gacgctcttc
     9661 gtacaagatg ctcatgagta ggcttcgctt tgttgtgttg tgtggcagct gatttttgcg
     9721 cgctgcttct gtgaacagtt attaagcggg cttttcgttt tcgtctatct ctttagctac
     9781 cggtcagacc attttttttc cagctctgtg accttgtctt ggttaactta atgttaaatt
     9841 gatgtaacat aatcacttgc gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg
     9901 tcctacctgt gcacgaggtc cgggaatgaa agatgaacgt cgccctattt gcgaagtggt
     9961 tgcagagagt atcgaacggt taattatcga cggcgtactg aaggtcggtc agccgcttcc
    10021 ctcggaacgt cgactgtgtg aaaagctcgg cttctcacgc tccgcactgc gtgaagggct
    10081 gaccgtgctg cgcgggcgcg ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc
    10141 acggcttaat cgggagcagg acaccagccc gctgatccat ctgttcagta cgcagccgcg
    10201 aacgctgtac gatctgctcg acgttcgcgc attactggag ggcgaatcgg caaggctggc
    10261 ggcaacgctg ggaacgcagg ctgattttgt tgtgataacc cgctgttatg aaaaaatgct
    10321 cgccgccagt gagaaccaca aagagatttc gctgatcgaa catgcgcagt tggatcacgc
    10381 tttccatctc gccatttgtc aggcttctca caatcaggtg ctggtgttta cgctgcaatc
    10441 attgaccgat ctgatgttta attcagtgtt tgccagcgta aataatctct accatcgacc
    10501 acagcaaaaa aagcagatcg atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg
    10561 gctgccgcac gtcgctcagc gcgcagcgcg cagcgcgcag cgcgcgatca tgtacggacc
    10621 gtgaaaaaga atctccacga tatcgagctg gaaggccacc atttgattcg ctcggcggtg
    10681 ccgctggaga tgaacaaggt gggaatgtga gcagagtttt accctatccc caaggcgagg
    10741 ggaccgcccc tgcacttaac aggatctggt gttaaatctg ttcatctata ccctctcccg
    10801 aatgaccgcc cctgcgctaa acaggatctg gtattaaatt tgcttttcat acaatcggta
    10861 acgcttgtac ggctccgccc caatgcgttc cagcatgtta ttcatgcctg tattggtttc
    10921 gaggatccat gacatctcca gcgcatcgat cttccggcgg gcaaacggat cgcgtaaggc
    10981 ttcaatcaat aacagcgcaa tcaccgggcc gatgcggctg aactgatact cgtcgcgcac
    11041 gcccatcagc ggtactcgcg cagttcgcac accgctgact ttcaaacgcc acagcaattt
    11101 tgcccagccg aagggaaaga gcgatccgtt cagatcggca atcgcctcgt tgatgttagg
    11161 caagccacaa taaacgcgca gggtgcagaa tcaatctcag cgatgtagat catatcgtcc
    11221 ggcaccagat atttaagttg gtcgcccatg gtcgcgaatt catgttcggt aaacggcaca
    11281 aatccccagt tgtgctgcca gccagagttg aaaatctcac gcaggatctg catctcttcg
    11341 gcaaaccgct gacgattgat gcagcgaatg gtcacctttt tgcgcacctg atccatcagt
    11401 ttttttagcg ccggagagaa agtgagatcg gttcgctgca tccaccacgc cagtaaatca
    11461 atgcctttgt gataacccag ttgttcaata tgcgcggcat accacggttt gccgtgtggc
    11521 atcatcgcac agggtggtgt gtcaaaacct tcaatcagta atccgctttc ctgattgata
    11581 ttcaggctga aaggcccgct gatcttactt gcaccttgtg acttcaacca cgcttccgct
    11641 gcgccaaaca acgcggcaaa atcctgcgga tcatcaatgg cgtcaatcat gccgaaatga
    11701 ccggtatctt caccgtaacg ctcacggtgc aaggtatcta tttgcgcggt aatacgccca
    11761 actatctgcc ccgctttttt tgccacccac gcctgccaga tgatatggtc cgtccccgga
    11821 tttttcgcag acaaatgctc gttgcgttca atgaataaag ggggtatcca gtttggatcg
    11881 tcgggataaa gtgatgacgg aaaagcgata aatgccttaa ggtcattttt attaattact
    11941 ttttcaattt taatcatcag cagatcgcat tccatatctc tgtggataac aaataattaa
    12001 tggtgaaaaa caggttgcaa cagcaacgag cggcatctta ataatcaact caatgaatac
    12061 aaacgacgaa taaatagtat taattaatga ttatgataag gtcagaggct acacagaaac
    12121 gcctcaatgc cttttgtcaa tttaaggcga ccggacaagg cttcttctta atatattcat
    12181 aaattaaata tcattcataa taaaaacaaa aaacttaaac tcattattta aatcaatgtt
    12241 cattttcaca ccgatgaaaa tactatctca tttatctttt atgcctatat aatccaccgc
    12301 attattgtca ttttattttt gatatccaat tttttcgtgg tgtatatgtc taataaaatc
    12361 tttacgcatt ccctacctat gcgctatgcc gattttccaa cgctggttga tgctttggac
    12421 tacgccgctc tgagtagagc cggaatgaat ttttatgaca gacgttgcca acttgaagat
    12481 caactggaat atcagacatt aaaaacgcgt gccgaagctg gtgcgaagcg gttgttatcg
    12541 ctgaacctga aaaaaggcga acgcgtggcg ctgattgccg aaacgagtag cgggtttgta
    12601 gaggcttttt ttgcctgtca gtatgctggc ttagtcgccg tcccgttggc gattccaatg
    12661 ggcgtcggtc agcgggattc ctggagcgcc aaactccagg gtttactggc aagttgccag
    12721 cccgcagcca ttatcactgg tgatgagtgg ttgccactgg tcaatgccgc gacgcataac
    12781 aaccccgaat tacatgtttt aagccacgct tggtttaagg cattaccgga agccgatgtt
    12841 gcgctccagc gtccagttcc gaacgatatc gcctacctcc agtacacctc cggcagcacc
    12901 cgttttcccc gtggcgtcat tatcacccat cgcgaagtga tggctaatct acgtgctata
    12961 agccacgatg gcattaaatt acgccctagc gaccgctgcg tctcctggca gcctttctac
    13021 catgatatgg gactggtcgg ctttctcctg ccccccgtcg ccacgcaact ttcagtagat
    13081 tatttgcgca ctcaggattt tgccatgcgt cctttgcaat ggcttaaatt gatcagtaaa
    13141 aatcgcggca ccgtttccgt tgcgccgccg tttggctatg aattgtgcca acgccgcgtg
    13201 aatgaaaaag atctcgctga actggatctt tcctgctggc gcgtcgctgg tattggtgct
    13261 gagccgatct ccgcagaaca actccatcaa ttcgctgaat gtttccgtca ggttaacttt
    13321 gacaataaaa ctttcatgcc gtgctacgga ctggcagaaa atgcgctggc tgtcagcttc
    13381 tctgatgaag cctccggggt tgtggttaac gaagtggatc gcgacatcct cgaatatcag
    13441 ggtaaagccg tcgcgccggg tgcagagaca cgcgccgtat cgactttcgt caactgcggc
    13501 aaagcgttgc cggaacatgg tattgaaatc cgcaatgaag caggtatgcc ggtcgcggaa
    13561 cgtgtggtag gccatatttg catctccggt cccagtctga tgagcggtta ctttggcgac
    13621 caggtttcgc aagacgagat tgccgcgacg ggctggttag acaccggcga cctcggttat
    13681 ctgctggacg gttatctgta tgtcaccgga cgcattaaag atctgattat tattcgtggc
    13741 cgtaatatct ggccgcagga tattgaatat atagcggaac aggaaccgga aattcattct
    13801 ggcgatgcga ttgcttttgt taccgcccag gaaaaaatca ttttgcagat ccagtgtcgg
    13861 atcagcgacg aagaacgtcg cgggcagctt atccacgcgc tggcggcacg gatccaaagc
    13921 gaatttggcg tgaccgcggc tatcgatctg ttgccgcccc acagtattcc ccgaacgtcc
    13981 tccggcaagc ctgcccgtgc ggaagcgaaa aaacgttatc agaaggctta tgctgccagt
    14041 cttaatgtgc aggaatccct ggcataaatc aaactgtcgc ggtgacgggc gctaccgggt
    14101 ttatcggtaa atatattatc gataacctgc tcgcccgcgg ctttcacgtt cgcgcattga
    14161 cgcgtactgc ccgcgctcac gtcaatgata atctcacctg ggtgcgcggt tcgctggaag
    14221 atactcattc acttagcgag ctggttgccg gagccagcgt agtggtccat tgcgccgggc
    14281 aagtgcgcgg gcacaaagaa gagattttca cccgctgtaa cgttgacggc agcctgcgcc
    14341 tgatgcaagc agcaaaagag agcggctttt gccaacgttt tctgtttatc tcttcgctgg
    14401 cggcgcgcca tcccgagctc tcctggtatg caaaatccaa atacgtcgcc gagcaacgac
    14461 tggcagcaat ggcggacgaa attacgctgg gcgtttttcg cccgacagcc gtgtatggtc
    14521 ccggcgataa agagttaaaa ccgctgtttg actggatgct acgcggcctg ctgccacgac
    14581 ttggtgcgcc agatacacaa ctctcttttc ttcacgtcac cgattttgcg caagcagtgg
    14641 gtcagtggtt aagcgccgaa actgtacaga cgcaaaccta tgaattatgc gatggcgtcg
    14701 ccggtggcta tgactggcaa cgcgtacgac agcttgccgc caaagcccgt tgtggttccg
    14761 tgcgaatggt tgacattcct ctgccggtac tcacaggcct tgcggatatc agtaccgcgt
    14821 tgagtcgcct ggcaggtaaa gaacctatgc tgacccgctc gaaaattcgt gaattaaccc
    14881 acgccgactg gtcggcaagt aataaccgta tttctgaaga tatcaattgg tttcccggga
    14941 ttagcctgga acacgcatta cgcaacgggc tattttgatt gtcttgtttt aaaggtgtgt
    15001 taacgtttct atggtaaatc gtgaaatagt aatggattac atcctttcct gtttacagga
    15061 tttggtcgaa aacggtgtgg aaattaagcc cgacagcgat ctcgtcaacg atcttggcct
    15121 ggagtcgatc aaagtgatgg atctcttaat gatgctggaa gacagattcg atatttccat
    15181 tcctattaat attctgctgg atgtcaaaac ccctgcgcaa ttaatggaaa ctctacttcc
    15241 atggctggag aataaataat ggggctatac gataaatatg cgcgccttgc tggcgagcgt
    15301 ctgcaattta gtgacaacgg gttaacaccg tttggcacct gtattgatga agtgtattcc
    15361 gccaccgaag gccggatcgg caataaaaaa gtgattctgg ccgggaccaa taattatctc
    15421 ggtttaacct ttaatcatga tgccatcagc gaaggtcagg ccgcgttagc ggcccaaggc
    15481 accggcacca ccggttctcg tatggcaaac ggcagttatg cgccgcacct ggcgttagag
    15541 aaagagattg ccgagttttt caatctccct accgctatcg tttttcaacc ggttataccg
    15601 ctaatttagg cgtcatcagc gcacttgccg accataacgc cgtggtgtta ctggatgccg
    15661 acagccacgc cagtatttat gacgcctgtt ctctcggtgg cgcggaaatt attcgtttcc
    15721 gccataacga tgctaaagat ctggaacggc gcatggttcg cctcggtgaa cgggcgaaag
    15781 aggcgattat catcgttgag ggcatctaca gcatgctcgg tgacgttgcg ccgctggcgg
    15841 aaattgttga tatcaaacgt cgtctcggtg gttatttgat tgtcgatgaa gcgcattcgt
    15901 ttggcgtgtt aggcgcaacg gggcgcggac tggctgaagc tgtcggcgtt gaagatgatg
    15961 tggatattat cgttggtact ttcagtaaaa gtctggcctc gattggcggt tttgctgtcg
    16021 gttcggaagc gatggaagtg ctgcgctacg gcagccgccc gtatattttt accgcctctc
    16081 cttctccgtc ctgcattgcg accgtgcgct cgtcgttaag aactattgcc acacaaccag
    16141 agctgcggca aaagctgatg gataacgcca accatctgta tgacggtctg caaaaactgg
    16201 gttatgagtt aagttcacat attagcccgg tagtgccagt aataatcggt tcgaaagagg
    16261 aaggtttacg catctggcgc gagcttatct cgccgggcgt gtatgtgaat cttatcctgc
    16321 cgcctgccgc gcctgcaggc atcacgctgc tgcgctgtag cgtaaatgct gcccacagtc
    16381 gtgagcaaat cgatgccatc attcaggcat ttgctactct gaagcagtaa tgtgagcccg
    16441 ccgtggtgtt tgcgccacgg cgggtttaat gttgtctgtc ataccaatac accaggctca
    16501 acgccaccaa cacaaacgct accggtaaac cgagcgcggt aacaggtgcg cttaacgcaa
    16561 atagcaatcc cagattaacc atgatttgat agctgatcca ggtgagtaag cccacgatga
    16621 cgcctacagc gagacggcta cccatcccag gcgaacgcgg ggcactaaag gtaaacggca
    16681 cagccagcaa aatcatcgcc agggtcagga tgggttgccc cagtttttcc cacagtgcaa
    16741 ggcggtattc gctgctgggt tgcccggttt cctgcaaata atggatgtaa tgattaagct
    16801 gacggacaga aaaagtattc cccggcattg ataactcttc aagattcata ctggtgaagg
    16861 ccgattgcca ggcaagatta tctaatgttt ccagcgtttc tttgccattc agccattttt
    16921 tatgattcac accatgcagg acccacgttt tgtcgtcttc aatggtggca ctgcgtgcat
    16981 aaatgtagga ttcaagcgag agatcgtcgc gataatgaaa tatctccacg cccaccggct
    17041 ggccctgctc gttcagcgat ttcaccgtca caaattcatt accacgcctg gcccacagca
    17101 tattgccggt aatgtcatcg tcctccccca acgcggtggc ggtcgatttg atttgcaacg
    17161 cctgttgctg caatggcgac gccacccact catctatcgc gcctaacgaa acagtccaca
    17221 atatcccggc aaccagcgcc accagtgcaa tacggaagat agaaaacccc gtgctgcgaa
    17281 tggcggtaag ctcactgttt ttcgataact gccccagtcc aacaatacca cccaataacg
    17341 cgataaacgg cgaaagttcg accagtgtgc gcggtaaggt cattagcacc accagcaccg
    17401 cctgagtcca gcgataaccg cccgggctga caccatccag ttcgttaatc aggttgaagg
    17461 tggtaaaaag cggcagcaat agccctgcag cggcggcaaa accgagaaag agatgacgga
    17521 ttaaatagcg actgaaaaca ttcatcggga aaatttctgc agcaaagaga agtcacgtgc
    17581 gaccagtatc agcaggccca ttagcatgag tccgggcact aaccacaaac caggaatgag
    17641 gggaatcgcg ccgttagcca ccagtgtacg gcagacgtcg ccgccataaa aaatggcaat
    17701 aaacagcaac gtcagtggca ataacgttga aaatcgccct tgccgcggtt ttacccgact
    17761 taatgaaatg gccagcagcg ccatcaacaa tgcggtcagg ccacggcttt gtcgccattg
    17821 cagttcggca tgatcggcag gaaaggcgga gcgcgccagc tccgtgaccg atttcgcttt
    17881 gcgtttgacg ttcgggcttt gatccagcgg attcagatgt aattgcagat tacggtagat
    17941 ctgttcgttg tcgtcacgcc cctgatgatc gagaagatag gcagtgccgt tgtgcaacat
    18001 aacggtcggt ttctcgggtg atgggtcaac cacatcaacc gaaccggcgc ggaaaatacg
    18061 ggttcgattg gcagtagaag tataaatcag cgcgtcagtc aggcgattat tatcctgatc
    18121 aaccgtctgc gaaaggatca ttcgtccgtt atcgttagtg ttaaatttct ttgcccgcaa
    18181 ctggcgaaca tccagctccg actgcgactg ctgctccagt tgataaatct gcgcataagc
    18241 ccacggacga ccatacatcg acagaagggt gacaaaaata cttagcggta tcgccagata
    18301 aagaacggct ttgtacaagc gtcccggact gccccccgcc gcagagatag cggtaatttc
    18361 cgagtcggta tacatctgcc ctagcgtcac gccaactgac acatacagac caacaggtaa
    18421 caacatctcc agtgcaatca gtactttgta gaaaacgata tccagcacaa catcaagagc
    18481 taatgtccca tttgccgctt cggtcagata acgctgtgcg gagtagctgg cgaaaataaa
    18541 gatcagaaaa ccgacgataa tcagcaccag acggcgcgta ccacgcatga tgtagtgttc
    18601 aaccagcttc atctcactaa cgccctcaat cctttgagtt ttttattcat tgatcagttt
    18661 ctgcgccaac gcaatgacat gatctacctc ttccggcgtc aggctgccgt cctttgccca
    18721 ctgaatttgc ccggctttat tcagcaccag aatggttgaa ctctgctccg gcaagcgcca
    18781 ggcaacgcgt ccctgtccat tgccgtcaat aacgaattgc gcccacggat agcggcgttt
    18841 atttttttcg attttgccaa caacaaaata gccagtacca aagatggcat catcggtgtt
    18901 cacaatggtg gtgggctgaa aacgatcctg cggaaagttt gccgcttcaa ccgctttaat
    18961 taacagtgag ttctttttct tcgccgactt acgcccggcg atatactgaa taatgcggac
    19021 ttttcccgct aattgtgtgc tgttccactt ttggtagtga atttcgtcgt cgctattcag
    19081 aagcagctcg ccgccttcct ggatataaac aggggtaacc gttttgccag tgacgaaatt
    19141 gtgtgcctgc acacccgggg ctaagagcat gataatcaac gcaataatca aacgagatga
    19201 catcattgct ccggctatag cggaaaacgc gaaatcagca gctctaagcg ctgacaaata
    19261 tcgctatcca gcgccaacgg tgcataatac gactggcggt tttccatatc atccgccacc
    19321 atgtcgtcgt gtttatctcc catcaggcca ttaatggcgg cgaggaaacg ggaaatctgt
    19381 aacgcctctt tattttcagg atcaagcgat cttaaggtgt cgtaataaga aacctgctgg
    19441 ctgatgattt ttcgcacatc atgaaattcc ctgccggtta gtcgcggttt ggcaataatc
    19501 tctttcaggg tatgcatttg cttttgccga taggcaataa aactgcttac gctttctaac
    19561 tggctatggc gtaatgaata gttcaccaac gaccagaccg gagagcttaa ataaatccgt
    19621 aacaggttgc cgtaatacga cacaatattt ttcttaccgt tacgaaagcc gtcctgaaaa
    19681 cgccccagaa aaacggtcgt tttgccgaat aaaaatccgg cctgatgctt ctttaaatac
    19741 agccgttgcg caaaacgcag gtgcttgtag cgcgctcgca tgtatttaaa ttgtttgcgc
    19801 tcatcagcag tcagctcatc gcctttggct tgtttgcgaa ctaaacgcag taattcctga
    19861 cgatcaacgc cattgagcca gaattgcagg cataacgcgt aattatcatg aatgatttcg
    19921 gtggtacaac ttagtgcaat agtcggcggc aaactaacct gtgcatcaac gacatcatct
    19981 tcttcaatcg caacaaatag acgctcgctc gcctcaagcg ggaaatattt tctcatcatc
    20041 aggatttctc tccggctgcg ttataaagca aggaatggcg ctgaaaaaat gcctttaagg
    20101 ttggaattaa cgagagcgcc acgtccagac attgcccacc gaagtaagca aacgctgctc
    20161 ccagcacacc aaacagatga cagaacatga acagcaatac gatgtagccg attgaggcga
    20221 tggtttgcgc cacgagaaaa gcgcgctgtt ttcccgccat caatagcaat gactcctgcg
    20281 ggaagcccag catcgaaatc acaattgcgc ccagcatcac ctgaatcagg tcatacgctt
    20341 cgagatactt aacgccaaac accagcgaaa tgagcggctt gccgacgatc aacaccgcga
    20401 gagccaccag aataccgatt ccgcccgcca gtaaaccgga cttcacacct aacaaccacg
    20461 gtctggtggt gcgcggatct aaacgcatca cctctgggta aaaactttta cccagcaaac
    20521 ccgctggcgt tccggcggcg tcgaagaatg tcatggcgat tttaaataac ccggcggcag
    20581 cgggtcctaa cacgatcccc accaacactg tgctgcacga gttacgcgcc gaccagatgg
    20641 agtgggcaat gttggttgac cagacaaaac tccacgcgcc tttaatgtgt cgggcagact
    20701 caaacagatt caatttgaag gcgttatgga tatttctacg gcgtaattcg cgcgcggcaa
    20761 accaccagta catggtgccg ccaaccagat tcgacacgta ccaggcaata acaaaacccg
    20821 caaaaccaaa gtcaaaatac caggctgcga cgctccccgc tgcgcgcaga aaaggtttcg
    20881 tcgcctgctg tacagcaatc aaatcaaagc gatctaccgc acgcagaatg ccggtcggcg
    20941 tggaggaagc cattgaagga atgagcgtgc aatacagcgc cgccagccaa aagctttggt
    21001 catccagacc taatgaatgg gaaaggaatg gcagtaaggc aatgccgccg acaatcgcca
    21061 ccgcgccgct gacgatatcc agcgagaagg aaaatgagac gacattgcga aattgctgcg
    21121 gattattgtt ggtcaaggct ggcgttccgt actgaaccac cagctgccat gtctgaaact
    21181 taataaaatc gctgatcgac ttggcgtacg attgcacaat caccagtaca ccaaacatgg
    21241 cgggcgtcat ccctttaccg gcacacgaga gcgccaacag ccccagcaag gcgctctcga
    21301 cattactgga gcctaaccag gcgctattgc gaataatggt gcgaaacgcg ccatctgcaa
    21361 accaatgttt gatgttaaaa cccgccaatt cagcctgacc ttatcgttga tagtaaaaaa
    21421 tatcccgcca gccttaagtt aaacttcggc ggtcagaaac gatggcaacc agagaaaccg
    21481 ccttctgtac ctgttccagc acttcgctgt agggcgctct ggaatcaatc tcaagaattt
    21541 ttgtgccgtt atagccaatc gtcgacatga cgccgatttt gtcctgcagc tcggcatact
    21601 catggtcagg cttgcgggag atggcagtat caatatcaat ccccaaacga atgattaatt
    21661 caggacggta ttgcgccatt ttttggtata aactccgttc gcgctgcgcc agaaacatgc
    21721 tgattttccc ggtcgcacgt tcgacgccga tccccggtcc gtcataataa aagcccgaaa
    21781 tttcagcctg cgggaagcga tcgctgacca ccagaacgcc actttgcgcc agtcgctgaa
    21841 ccttgcgtag attcgccatt cttcgcagcg agaagcagta cataatcacc gccgcccata
    21901 gcgccggaga tttggttttc atgctttggg ttttcgagga tttggccgcc aatcgccgtt
    21961 ccagccagac gccaaccaac ggcaatcgtt tgattttgtc gccgtcttcg ccggagagca
    22021 gccccagata gcggcgctcg gtttgccagt gttgttgcag cgatttcacc aggtcggtgg
    22081 tcagcgtgga tttaccggtg ccatcacacc cgaccaccgc aatcagcccc ggaatgtagt
    22141 tgggctgcgg cgcggttgta ctattaacag tttgtgtttg tagtgcatcc attcgttaaa
    22201 tcatccccta tccattttgg tgatcaggaa agcgcggcgt gaattgcgcg tagtgacgct
    22261 tgcataattt catcggctgg ttgccgacca tccagttcaa ggatcttggc gccattgaat
    22321 gtcagttgcg gcgtaacggc gattttttcc tgtaacgctg ccagttggtg gtcaggttta
    22381 cgcgcaaacg cggtttgttc atcaatgcca agacgaatca acaatacggg taaataagac
    22441 gccatccatt ggtacagctt caactcgcgc tgccttaaca tttttatcca accgttaccg
    22501 cccgtggttt ttgccaattg cgggccatca aagcgaaaac ccggcacttc aacctgcggg
    22561 tagcggtcgg tgatgagcag atagccttgc tggcttttac acaacatttt gcgaaacttg
    22621 tacgcccgcc agcaggaaag cagatagata accagcgcag taatattgcc aggcggagtt
    22681 aagggctttt cgtgtacatg tgccgcttta cttcgcagat aacacccaca aggtgcgcca
    22741 ataacaggga gctgtgaaat ccattcgcca attcgcccgg acgattgccc gagataaatg
    22801 tgttctgttg gcattcttgc tgccagttca tttaccaggc ttgccgtgag ggtcgattta
    22861 cctgaaccat cacatcctac aatggcaata acacgcactg gcgtggaatt aattattgac
    22921 atattacgtt gattcacgaa aaacccggca gtaaataatg tattgaatat tacgtggtcc
    22981 gtgctcagaa tatccgttca accttattgt gacaatggct aattgtgaca ttgacctgaa
    23041 ttacgctttc ataaaaacat attaaccaaa taaatatttt taatggatat ttaaattaaa
    23101 ggatatattc atgcagtcaa taacacctcc attaattgcc gttattggta gcgatggttc
    23161 aggcaagtca acggtgtgtg aacatcttat taccgttgtc gaaaaatatg gtgctgccga
    23221 aagagttcat ttaggaaaac aggccggaaa tgtcggtcgt gcagtgacaa aattaccttt
    23281 gatgggaaaa tccttacata aaacaattga acgaaatcag gtgaaaacag caaaaaaatt
    23341 gcctggacca gttccggcgc tggtaattac agcgtttgtc gcccgtcgct tactgcgctt
    23401 tcgtcatatg cttgcctgtc gtcgtcgcgg gttaattgtt ctaaccgacc gttatcctca
    23461 ggaccaaatt cctggcgctt acgatggtac ggtgttccca cctaacgttg aaggtggtcg
    23521 ttttgtctca tggctggcaa gccaggaacg taaagcgttt cactggatgg cgagccataa
    23581 gcctgatctg gtcatcaaac tcaatgttga ccttgaagtt gcctgtgcac gtaaacccga
    23641 ccataaacgg gaatcgctgg cgaggaagat tgccataacg ccacagttaa cctttggtgg
    23701 tgcacaactg gttgatatcg atgccaatca gccactggaa caggtgttgg ttgatgcaga
    23761 aaaagcgatt acggatttta tgaccgcgcg tggttatcac tagtcaaaaa tggaaatgcc
    23821 cgatcgccag gaccgggcat tttcaggaag gttaaatcaa ctgcaatgct atccagtaca
    23881 gcccaccaga aagaaaaatt gccgccggta aagtaaacac ccacgccatc aggatgctgg
    23941 ttaccgtttt acgctgtaac ccaccgccgt ccaccaccat cgtccctgct accgcagacg
    24001 agaggacgtg tgttgtggag acaggcatcc caatataact ggcaaggccg atagacactg
    24061 ccgccgtcat ttgtgccgcc atgccttgcg catacgtcat gccgcgctta ccaatcttct
    24121 caccgatggt catcgctaca cgacgccagc caatcatggt gccaatgccg agcgccagtg
    24181 ctaccgccat gatgatccac accggagcgt actcaatggt gcttaacata tcgctgcgca
    24241 gttttttcag caggttctgg tcttctttac tgacgcctgg cagtttcgcc agcttcgcgg
    24301 aggtatcgga gatgcacagc ataatgcggc gcagctggct gcgctgactc acgcttaacg
    24361 gctcgtaact ttccatattg cctggcagca tcgttttaac gcgcgcaatc gcatcaaagg
    24421 tatttgccgg atgacagtga aactctgtta cttgcgtgcc atcagtcgat gctgcaggca
    24481 atggaggttc catcgcaatc aacttctgcg gcagttcagg atgctgttgc aggtagtgtt
    24541 cgaagttggt aacggcatcg cgggtacggg taatttcata gccggacgca ttcatattga
    24601 cgacgaagcc agcaggggca atccccacca gtaccagcat taccaggccg atccctttct
    24661 gcccgtcgtt cgcgccgtgc gaaaacgcca cgcccgcagc ggaaacaatc agcgcaatac
    24721 gcgtccagaa tggcggttta cgtttgccct ttttcttttt gcgatcttcc ggaatgcggt
    24781 gaatacggtc acgctttttc gtcccactcc agaaacggcg cagcaggaaa atcagaccac
    24841 ccgcaatgac caggccgacg ataggggaaa caatcagcga ggagaaaatt ttggttactt
    24901 cacgcaggtt taacgcatcc atcaccgatg agccggttaa cagcgcgttg gttaaaccga
    24961 tgccgataat cgcaccaatc aaggtgtgcg aactggaggc cggtaaacca aagaaccacg
    25021 ttcccaggtt ccagataatc gccgccagca gcatggaaaa gaccatcgcc aaaccgtgag
    25081 ttgaccccat attcagcaac aaatcgattg gtaacatatg gacaatggca taggcaacgc
    25141 taagtccgcc caataacacg ccaaaaaagt taaaaaatgc cgccatcacc acagcaagtt
    25201 gtggctgcat ggcacgagta taaataacgg ttgccaccgc attcgccgtg tcatgaaaac
    25261 cattgattgc ttcgtagaac aacacaaatg ccagagcaag taataacaaa agccccgtat
    25321 atatatcaag gccaacaaat aaatttagca tatattagat tacgccattt tgaatttacg
    25381 aacggacgca ttatcagtga ctttaacggc atgggcaaag tgaaatatca ttttttaacg
    25441 ataaaaagag tgttatttct tgtgtatcag acaataagca ttcattcaaa acctttagaa
    25501 ataatcaaaa cacaggtatt ccactggtta atatattcac atatgaaatg aataaatatt
    25561 ggaatatata aatattgaat attttgatta atccccgtac taattattct tcataatcag
    25621 tacggggttg ctacaacatg tattactttt tcaccacaat taacggttca atatcactct
    25681 cttttttgat gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag ttgccgccaa
    25741 cggtgaaggt acatacctga atgtatttac cgtccacttt cggcaaacac cacagttgct
    25801 gatagatgtt tttctgctcg gcaaatttac cgctggtttt gtccagcacc tcttcatgat
    25861 ggctgaccag gtcgatattg ctaccacagc gaccggcgat cggtttcact gcgtaacctg
    25921 ttttcaccaa ttcatcatta acagtgaaat cggtatccag caggtaacga tggtgcggga
    25981 acagcgacca gaggatcggc agaatcgctt tgttgccggg gatcaccgtc cacagcggct
    26041 caaagaccag cacttccggg cgcagcaata cgtcgataag acgcacttcg ttttgcggat
    26101 gaccggtacg gattggcacc gcagcaaact cacggtcgct aacttcacga atctgatcaa
    26161 acgcggtttc ccacgcccag gttttccaca cgcagttaac cagtcgccct tccccatcaa
    26221 tcagttgccc ggcagcatcc cagcccagtt catccagccc gcgcaagata cgcgtttcaa
    26281 agcccgcctg gtgcagcgcc tgctccataa actgcgcgtg atagttttcc tcgatatctt
    26341 tgtcctgcat gatatggaca aacggacgtg cacgactgtg tttccaggca ccagccagtt
    26401 cgttaatcag cccttccgcc ggattgaagc cgttgccttt atagccctgc tccgcccaac
    26461 gttcgaggat caagcccgct tcggtatgac aggaggcgga gtcggcgttg tattcgtaaa
    26521 ccttcaggcc acgctcatcc atgcagaaat ccatacgacc agtgatcata tggtgacggc
    26581 gacgctgcca ggagagacgc aaacgtggcc agaggatttt cggaatgtcg aacagcgcca
    26641 gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag atacatcaga tgcagctcgt
    26701 tggtggcttt aattagctcc tgctcggcac tctcggtaat ggtgtagtaa tgatacggat
    26761 cctgattgat cacctgaccg ttggcctgca cataggcgtt ttgtagcgga tctttttcat
    26821 ccagccattt accgtcaaac tggcctttgt tttccagacg cgctccactg attttcagca
    26881 gctcgcctgc aatttccggc tgcggtaagc tgtattcagt atcttccgtc tggatcatcc
    26941 agcccagaat ggtggtgtca tcaaaagtgt ctttaagggt atagcagccg ttttcgacca
    27001 ccatctccag ctcgcgcgtc cactgttgcc cttgcggcaa cggggaatga atcacgttct
    27061 gttccgcaat acggactttg ttgccatgca attgggtaat gatggcgaca tggccagtgt
    27121 ctttaaattc accgccttta tcccagataa gaagcgcacc cgcgaccggc gcacgcggcg
    27181 agccgttagg aaatgcctgc aatggcagga tgttgtcatt aaccacttct cgcaggaagc
    27241 gcagcgagaa aatctcccac gccataccca cgtcagtaaa gaccacaccg taattcagaa
    27301 agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg gcccatatat tcgtcgtcga
    27361 tatagctacg gaatacggcg tcatcttcgt attcctgcgg atcgagagaa ctgtaatctg
    27421 aagagtagat tgctacccca cctggggcgt agcccaataa tgtcccgaac ggggcatcct
    27481 ggctggtcgt tcctttgctc atcactttac ctttaacaat acaacctaag cagttggcgt
    27541 aattgttgtg ctctgattac ctgctcagcg acattaaccg gacagaggtc agactaatca
    27601 tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg tacagcaagt
    27661 gacctgctac actgcttcaa cactaccact cagaaggcaa ctcactatga cagacaatac
    27721 ttatcagccc gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt tcgccaatat
    27781 caatcgcccg ttttctggtc cgacgcatga aaaaacgctg cccgttggca aacatccatt
    27841 gcaactttat tcgctgggaa cgccgaacgg tcagaaagta acgattatgc ttgaggagct
    27901 actggcgctg ggcgttactg gtgcagagta cgacgcctgg ctgattcgta ttggcgatgg
    27961 cgatcaattc tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc cggcgctgcg
    28021 cgatcatacg cataatccgc cgatccgcgt gtttgaatct ggttcgatcc tgctttatct
    28081 ggcggagaaa tttggctact tcctgccgca ggatctggca aaacggactg aaacgttgaa
    28141 ctggctgttc tggttacagg gtgccgcgcc gttcctcggc ggtggttttg gtcactttta
    28201 ccattacgcg ccggtgaaaa ttgagtacgc catcaaccgc tttaccatgg aagccaagcg
    28261 cctgctcgac gtgctggata agcaactggc gcagcataag tttgttgcgg gcgatgagta
    28321 caccattgcg gatatggcga tttggccgtg gtttggcaac gtggtgctcg gcggcgtgta
    28381 tgatgccgca gagtttctgg atgcgggaag ttataaacat gtgcaacgct gggcgaaaga
    28441 agtgggcgaa cgtccggcag tgaaacgtgg gcgtattgtt aaccgcacca acgggccgct
    28501 gaacgagcag ttgcatgagc gccatgacgc cagtgatttt gagacaaata cagaagataa
    28561 gcgtcagggg taagggttgg tgttcgtcgc agcaagccat ccaggccgga taaggcgttc
    28621 acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc ttatcaggcc
    28681 tacaaaagct aatcatcacg cgctggtaat gtcgtaatcc atttggcgca gtgcgtctaa
    28741 tgtggctttg gcttcatctt cgtcgatgat gctcatggca aatccgacgt gtaccagcac
    28801 ccactggccc agcagatcgg cagggttacc ttcgcaaatc agggcgatat tcacgtcgcg
    28861 cttgatacca catacttcaa cctgcgcaag ctggtgaata tcttcaccga cagccagcac
    28921 ctggcctgga acgccaatac acattgttaa ctccggttat ttcgattgcc ggacaaacgc
    28981 gaaaaagaac ctttagcggg tgttccctct caccctaacc ctctccccgg aggggcgagg
    29041 ggaccgtccg tgcgcttttt aggcgttgtc ctcagtctta ttcaacttca atacttttga
    29101 cgatcagcga atcgccggta tcgacacgca accgctcgcc gtgacagagc ggacactgcg
    29161 catcgtgctg atgaatctcc accacctggc tgcaatccca gcaccaggcc tgggcgggtt
    29221 tatagacgat atgtaaatcg cacccttgcg ccaccgttcc gtggcagaca atttcaaaac
    29281 taaaacggac ggcgctctcc tcaacgcagg agagcgcgcc aatttccagc cacacggcgg
    29341 tgacgcgctt aacatcgtgc tgctccgcct gccgttggat aatttcaacg gcgctctggc
    29401 aaagcgacaa ctcatgcatt ttcgctcctc ctgcggccaa acagcagggc gcgacgccct
    29461 gcgtgtggta catccggatt ggtgactggc aacgaaagga tcattcgtgc gcagtcatcc
    29521 gtcaggcgtt ggccttcttc aatcgacatg ctgtgcgaaa gcggcgacat cagcgaacag
    29581 gagaggtatt gcgaaacacc gtccagttcg ccaacggtaa aggtcatggt gccatacggc
    29641 agttgcagac caattttttc actcactttg cgcagcggcc agagttgatc cgggccgggg
    29701 aagataactg cactcagcat ccacggggtg atcacacacc ccgtccactg accttcgaac
    29761 agcgtaaaat cagaaacata caccggcatt gaaggatgca gaaaagagag atcgtgcatc
    29821 gaacgccggg caatttcttc aaacgctgcc tgtacttgcg ccttcgggga ggtctggaaa
    29881 cctgctatct cttcagtcat gaatcgcctc ccgtgggatg gcttccacgc ctgattcgcg
    29941 cagcgcagcc agaacctgct caagcgcagg ttcaatcatt gcttcaaccg tcggcgttaa
    30001 gccgatgtgt ggctccagcg attccgggat cacgccgacc agggtcagct ttttcggaaa
    30061 ctcaccggtg aagcgcaggg ccgacaatac gtcggccagg ccaagctgat gcggagagat
    30121 tttgttggta aacaacgccg gaacttcttc atcccgcagg atcatcatcg ttcccggagt
    30181 gttctttttc gacacaatgg catccgcaat aatcagatga tcgcgatttg ccatgtcgcc
    30241 aagcagctcc attcccgccg tgccgccatc gaggatctca acataatccg gcagaatgta
    30301 tcgttgctct aacgcttcga caatccgcac gccgatggct tcatcggtca gcaaaatatt
    30361 gccgacccct aagactaaaa tacgcattac agaaccttca ctgaaaccac ttcgttgccg
    30421 tcggcatcca ctacgtgtac cgcacaggcc atgcacgggt cgaaggagtg aatggtacgc
    30481 accacttcca gcggtttatt cggatcggca accggtgtac ccaccagcga ctgctcgtaa
    30541 ggaccgacgt catcattgaa gttacgcgga ccagagttcc aggttgatgg aacaaccgcc
    30601 tggtagttgc tgatgatacc gtctttaata accatccagt gagagagcat accgcgcggc
    30661 gcttcgagga agccaacacc tttgaactca cccgttgccg gaatgttcgg cttcacaaag
    30721 gtggtgtgat cgcctttgcc gatattggtg atcagtgcac tgtattggtt ttgcaggata
    30781 tcctgcaatt cacagcagtg aacggtacga ccaataatac ggcccagcgt ggagtgcagc
    30841 tgtgccactt ccagcgtgtt gccagtcagt ttctgataaa tcgcaacgat ttcattcagt
    30901 ttgttctggg tagattcgcg acctgccgcc agtttcacca gcatattagc cagcggaccc
    30961 acttctaccg ttttgccgta gaaagtcggt gatttcaccc aggaatattt cccgtcgtca
    31021 gaccaaccat cataagccgg aatggtggta ccttcccacg gcgcctgtgg cgcttcgtct
    31081 ttataccagg agtgcttcgc gctttcctga atgcctttga tcaggtattc atcggaatga
    31141 gaagtgatcg gacgatacga ggacagatcc gcattctcaa tgtagcctcc cgggaacagg
    31201 aagctgccgt ttttgctgtc ggtcgggaat tccggcacgc tcaggtagtt caccgcacct
    31261 ttaccgcgcg tcagccattc cgggtagaac gcggcgataa ccgcggtatc aaccttataa
    31321 acctgctcaa caaagtcgct cagtttgtcg atgaaagact tgatgtacat caggcgctca
    31381 aggttcaaca cgcccaaccc gtcgaggttg attgggttcg cgacaccacc cactgccagg
    31441 ttctgaatgt gcggcgtttt accgcccagc agcgccacga cgcggttagc gtcacgctgg
    31501 cactccagcg cctgcaggta gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc
    31561 atcgccgggt gaccccagta gccattggcg aaaataccca actgaccgct ggcaaccaga
    31621 tctttgatct tgttctgaac tttagtgaac tcttccggac tgttcagatg ccaggtcgaa
    31681 acgcctttca gcatttcgga ggctttggtt gggtcagctt gcagtgcaga agtgatgtcc
    31741 acccagtcca gcgccgaaag ctgatagaaa tgaacgatat ggtcatgcgt ggtgtgcgca
    31801 gccagaatga tgttacggat gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactt
    31861 tctgccgcac gaacggaaga cagcgcgtga gtggtagtac atacgccaca gatacgttgc
    31921 acaatcatcc atgcatcgcg cggatcgcgg tttttcacga tctcttccat gccgcgccac
    31981 atggtaccgg aagcccatgc tttcgaaacg acgccatttt cgatttcgca atcgatgcgt
    32041 aaatgcccct caatacgggt taccggatca atagtaattc tctggctcat gctttgctcg
    32101 cctcatgacg attatgatcg ttttgtttta aaggaggaag tatcggcagt agacgaatga
    32161 gtacgatgta agcgcaaatc tcaatagcca caaaaccaat agaaatcaac agttcttccc
    32221 aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc caccaacgaa taggtcagac
    32281 gccaggttgc acaacctaac agtgcgctca gtgctgacag gaacagcatg cgggaatcat
    32341 tacgcagctt cgccacacgc agaacgacca gcgggaagag catcagcagg acttcaatcc
    32401 agaacatcac ggagtagaag tcaccggcaa acgctaacga cagcttgtcg cgatagataa
    32461 gctcgccaaa gcgcagcccg atgaaaatcg ccagcaacac actgatggtg ttggtcagct
    32521 tgacgaacag gctcttttca tccggaccgt tgccacgcag acccgcctgc accagtgaac
    32581 cttcaaagat gacaatcgag aagcccatga tgaacgccgt cagcagcgag aacagcggca
    32641 acatttcata gctctgccag agcggatgca ccttgtagcc cgccgagatc atcagcgacc
    32701 ccattgaaga ctggtgcatg gtcggcagca gcgcaccgag cgcgatgatg aagaacatca
    32761 ccttgttcag acgttgtagc gacaccttcc agcccagacg ttcaaacagt gccggagcaa
    32821 actccagagc catcacgccg atgtagatgg tcatacagac cgccgtctcg aacagtaccg
    32881 agttcacgtt gaagtgaccc ggaatgtaga agtacggcag gttccagtag cgacccacgt
    32941 cgatagtgat cgacaagcca cccagtgagt aaccaaacag actcgccaac agcgccggac
    33001 gcaccagcgg atggtattgc ccacggttaa agacgtatac cgcccacgcc agcgcccagc
    33061 cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc gatccacacg ccccacggga
    33121 agccgccgtt caggtcagag acagagccca gaccgaacac cagacgcttc acaatcagga
    33181 gcatacagat gacgattaac ggtccaaaaa tcatgaccgg tttactgatg attttgccgc
    33241 ccagcggttg tggatcatga ctcatgatcg tctcctccgt cgtgatggtc gtttttggtg
    33301 ttgcgacgaa ccagcacggt taagcccgcc agcacagcca gtggtagcat catgccttta
    33361 tacagggtgt gttgaatatt ttcggaacgc gcaccggtag aaagatcgtc cagtttcggc
    33421 aggtcgagat tttcataagg cacacccgtc agtaccagaa cctgagtacc gccgccctct
    33481 ttctcgccgt acagatgcgg ataatatttc ggcaccgtat gcaggtaagt gtcgccagat
    33541 ttcagcgtct gacgcggata gtggtattcg ctgccaggct tcagcgccag acgttttttc
    33601 gcctccgcca tcagctcttc acgcgtaccg aaaatcaccg cgcccgccgg gcacacttct
    33661 acgcagccag gcagaccgcc tttatcgaga cgttccacac ctttctggtt gcacagctcg
    33721 cacttatgca gcgcaccaaa cgggttgttg tagtcgtact tcggcacgtt gtacggacag
    33781 gcgaccatgc agtaacggca accggtgcac acgtctttgt cgtaatggac aatgccggtt
    33841 ttcggatctt ttttcagtgc agagaccggg cacacagaga cacagttcgg atcgacgcag
    33901 tgcatacact gtttcttaat gtacgcgtag ccgttctcct cctggtcttt gttgacccct
    33961 gtgccgctgg tccacacctg aatgatgtta ttggtatacg gcgacagttt gtcgttgttc
    34021 gaccaggtct gttccccttg cgggttacgt tcagggaagt tgatatcctg acacttggtg
    34081 acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca acatccccag cgatcccgga
    34141 attggcgggc ggttttcagc agccgcatga ctgacagacg gcagcgcgcc cgtcagcaat
    34201 gccccgcagg aggctgcttt aataaaatta cgtctgttca cggttattct ccccgtgagt
    34261 cagcgttatc tttcttttgc tgacgaccca gttcacgcac cgccatcacg ctgacaccgg
    34321 caaccagccc aaccacaccg ccgagcaaac caatagcgcc tgcagagacg ttgccgccct
    34381 ctttagcgtt aacatccggt ttctgtgaac gcggagtctg attttcgacg ttggcaagct
    34441 gatggatgcc tttatggaag ccgatacctt tttcgttaca gccatagcac gggtgaccaa
    34501 tcgccaccgg ccacacaccg ccaacatcgc agaattgcag cgttgagcag ttgccgtaag
    34561 tttctggccc tttacagccg aggtggtaca ggcaccagcc ttcgcggtgg ccttcatcac
    34621 cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg acgttcgcag tgttcgtgaa
    34681 tcagacggcc ataggcgaag gtcggacggt ttttgtcatc cagtttcggc ggtttgccgt
    34741 aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg gttcggcggg cagcccggaa
    34801 tattgataac ggttttgcct ggcagaactt cttgcaagct gactgcgcca gttgggttaa
    34861 ctccagctgc ggcaacaccg ccccacgcgg agcaggaacc aatagcgata atggctgctg
    34921 agccttccgc cgctttgcgg atgtgatcca caatcggctc accggcaacc atgcaataaa
    34981 taccgttatc ttttaatggg atggaaccat ccaccactaa cacatactgc cctttgtact
    35041 tctcgagagc gttatgtttg ttctcttcga cctgatgacc gaaggcggcg gaaagcactt
    35101 cgtgatattc cagagagata gtctccagca cgaggttttc taccgttgga tgcgttgcac
    35161 gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc aatccagata actggcggac
    35221 gctgcgggtt agtaaccgat tcggccatct ctgcagcggc tttgctactt aaccccatgg
    35281 tggcggctaa tgctgcacaa agcttcatga aatcacgacg gttaatgccg tgagaatgga
    35341 tgagggtgtt atctccagtc atttatagtt attccgttgc gaggacctgg catatatttt
    35401 gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt taccacacat ttattatggt
    35461 tatcgatatc atgatactgc ggcggggcgc taaaacgaag ggaagaagca ttagtgtaat
    35521 taattaaagc aagataatac gtatgtttga tcaattttcg aggcaataac cggccataaa
    35581 aaagcgcaac gtagaacagg aattattctc acgaaagaaa tccattacca tctctttatc
    35641 cagtccatag cggcgcgtca gaatacccgc ttcccaggcg ctaagctggc gatctccggt
    35701 cttttcgtac atccggtgtg agtagcctaa tacaaaaccg cgtttataat cggcacagaa
    35761 ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt aatcccgcca ttaagccttt
    35821 accaaaatgg ttattcatcg ttcacctcat tcgttatata tcgccatata tagcgatatt
    35881 ttaggcaaag aacgactttt gcgcacgcta ttttttcata cttgtttaag acgtaatccc
    35941 gtaagagata aacctatcag gagagatatt tatcgaacca tttcagtgtc ctttgccagg
    36001 caagatcggc ggcagatttg tcataacggg gcgtggaatc attatggaat ccgtgattaa
    36061 cccccggata gatataagcc tcataaacct tattattgtc tttcaacgcc gcctcgtaag
    36121 gaggccagcc ctcgttgatt cgggtgtcca gttccgcgaa gtggagtagt aaaggcgctt
    36181 caatcttcgc cacatcggca gtgggtgcct gacgaccata aaacggcacc gcgcaggcca
    36241 gttccggata cgcgacagcc gccgcgttcg atacgccacc gccatagcaa aatccggtaa
    36301 tacccacttt acctgtcgct tgcggatagc gttgcataaa ctcaattgcg gcaaagaaat
    36361 cattcatcag tttggttgga tcaacctgct gttgcagctc acgaccttta tcatcatttc
    36421 ccggataacc tccaacggaa cttaatccgt caggtgccag ggcgatatac cccgccttcg
    36481 ccactcgccg tgccacatct tcgatatacg gattcagtcc acgattctca tgcaccaccc
    36541 ccacggctgg cgttttgccg ctcatttttg cgggtttcac cagataaccc cgtacctcgc
    36601 cgtgaccatt tggcgaagga tacgtgatgt actcagcaac aatctccggg tcggtaaact
    36661 ctacctgagt cgccagcgca taatttggct tgagcaaatc aaacaacgct aatgccgtca
    36721 tcccgcccac cgcatacttc gccgcaaggt tgaggaactc acgtttcgaa attttcccgt
    36781 gaacgtaata gtcgtagtaa tccaacaact cttgtgggaa atctttggcg gttaaacgcg
    36841 gcatcgttgc attcctcaac tggtgttttt ttaagcaaag cataagcgcg tgtttttgcc
    36901 cagtttttcg tcactctgtg agccagacta cgggatacgc gctggcgaat cgctaaacta
    36961 gaaacattgt ttcgaaattg aacggaggaa aggagaggtc atggtctggt tagcgaatcc
    37021 cgaacgttac gggcagatgc agtaccgcta ttgcggaaaa agtggtttac gcctgcccgc
    37081 attatcgctc ggtttatggc acaatttcgg tcacgttaac gcgctggaat cacagcgtgc
    37141 aatcctgcgt aaagcgtttg atttaggcat tacgcacttt gatttagcca acaattacgg
    37201 gccgcctcca ggaagcgcag aagagaactt tggtcgcctg ctgcgcgagg attttgctgc
    37261 ttatcgcgat gaactgatta tctctaccaa ggctggctac gatatgtggc ccggcccgta
    37321 cggctctggc ggttcacgta aatacctgct cgccagcctc gaccaaagcc tgaagcgtat
    37381 ggggcttgag tatgtcgata tcttttactc tcatcgcgtc gatgaaaata cgccgatgga
    37441 agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt aaggcgctgt atgtcgggat
    37501 ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag ttgctgcacg agtggaaaat
    37561 tccgctttta attcatcaac cttcgtacaa tttactgaac cgctgggtgg ataaaagcgg
    37621 cctgctggat accctgcaaa ataacggcgt gggctgtatt gcctttactc ctctggctca
    37681 gggattgctg accggaaaat atctcaacgg cattccgcaa gattcacgga tgcatcgtga
    37741 agggaataaa gttcgtggtc tgacgccgaa aatgcttacc gaagccaacc tcaacagcct
    37801 gcgcttattg aatgaaatgg cacagcagcg tggacaatca atggcgcaaa tggcgttaag
    37861 ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt ggtgccagcc gcgcggagca
    37921 actagaggag aacgtgcagg cgctgaataa tctgacattt agcaccgagg agctggcgca
    37981 gattgatcag catatcgccg atggcgagct gaatctgtgg caggcgtcgt ccgataaatg
    38041 acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga tcagtgatta tgacgagtca
    38101 gtcggtcaag atagcccatc acaaatgcag aaagcacaaa cgtcagatgg ataatgacgt
    38161 accacatcag tttgttatcc gggacatttt tcgcatccat aaagacgcgc agtaagtgaa
    38221 tggaagaaat tgccacaatc gacgctgcta ctttgttttt cagcgacgtt gcgtccattt
    38281 tccccagcca cttcagcttc tctttgttct cggagatatc cagctgtgag acgaaattct
    38341 cataaccgga aaacatcacc atcaccagca aaccgccaac cagcgtcata tccaccagcg
    38401 acagcaacac gaggatcaaa tctgattccg ccatcgagaa gatattcggc agtacgtgaa
    38461 taatctcctg gaagaacttc agcgccaggg caactaacgc cagcgaaagg ccaaagtaca
    38521 cgggggcaag cagccagcga gaagcataca ttgcattttc aagaaaacgt tccatacatt
    38581 cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt gagtaaacag cacgcaacag
    38641 caaatcaaca tatacgttaa acctttgtcg cataattctg aggtttaggg atcttcctgg
    38701 ttcccttatc aacgcatcga gagcgtctac ccttaaagga cgttgaacct gaagggagaa
    38761 aaacgatgtc tcatttaaaa gacccgacca cgcagtatta cactggtgaa tatcccaaac
    38821 agaaacaacc gacgccaggc atccaggcga agatgacacc gataccggat tgcggcgaga
    38881 aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc actggtgaca gggggcgatt
    38941 ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga aggggctgac gtggcgatca
    39001 gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg
    39061 gacgcaaagc cgttctgctg ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg
    39121 ttcacgaagc gcacaaggcg ttaggcgggc tggatattat ggcgctggtc gccgggaaac
    39181 aggttgccat tccggatatt gcagacctca ccagcgaaca gtttcaaaag acctttgcca
    39241 ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg
    39301 caagtgtcat caccacttcg tcaatccagg cataccagcc aagtccgcat ttactggact
    39361 atgcggctac gaaggcggcg attctgaact acagccgtgg cctggcaaaa caggtcgcgg
    39421 agaaaggtat tcgggtgaat attgtcgcgc caggccctat ctggacggca ctgcaaattt
    39481 ccggcggaca aacgcaggat aagatcccgc agtttggtca gaaaacgccg atgaagcgtg
    39541 cggggcaacc ggcggaactg gcccctgtat atgtttatct ggcaagtcag gagtcgagct
    39601 acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca tttaggttaa aaaaatgccc
    39661 ggttgtgaaa agcaaccggg catcattgtg aattcactct tacctgatgt ggcgaaaacg
    39721 ccttatccgc ctacaaacgc atgaagattc aacatgttgc aatagatttt gtaggcctga
    39781 taagcgtagc gcatcaggca ttgagcacct aatgcctgat gcgtcgtgaa cgccttatcc
    39841 ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat tttgtaggcc tgatgagcgt
    39901 agcgcatcag gcaattctac tttacttcgc tttggcggtt tcttcgccga ccagacctat
    39961 cttcaggtaa cccgcctgat gcagcgtatc cattaccttc atcaacgtct cgtaatcgac
    40021 ggttttgtcc gctcggaaga agatggtggt gtctttcttg ccttcggtta acgcattcaa
    40081 cgccgtaatc attgtttcat cggtgaccgg atcgttaccg ataaacatcg agttgtctgc
    40141 cttcaccgac agataaaccg gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg
    40201 caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc accataaaga taatcagcag
    40261 aaccaacatc acgtcgataa acggcgtcac gttgatatca tgcatttcgc cgttatcgtc
    40321 gaggttttcg ttaagatgca ttgccatcgg acattatcct gcgcgtaatt tttgtgcgac
    40381 acgaaccgga tgcgcagcgg cgctggcttc cagatccagg tcacggcttt gcagcaacaa
    40441 tacctgcgct gcaacatcac ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac
    40501 gttatagata acgaccgcag gaatcgctgc cacgaggccg attgccgttg ctaacagagc
    40561 ttctgcgata cccggcgcaa cgactgccag gttagtggtc tgcgtttgcg cgataccaat
    40621 aaagctgttc atgatgcccc agaccgtacc aaacagacca acgaacggag aaatcgcgcc
    40681 gatggttgcc agatagccgt taccgcggcc catttgacga cccactgcgg cgacccgacg
    40741 ctccagacgg aagctggtac gttctttaat accttcgtta tcgtcgctgc cttctgacag
    40801 ttccagctca ttctgcgctt cattgagcaa atgcaggctt aagcttttgc taccaaaatc
    40861 agcggcgata tcgttggcct ggtttaagga acgtgcttca gccagcagtt gctgctcgcg
    40921 cttaaggcga cgcttctgat tgaagaactc tacgctctta ctgaagaaga ttgcccaggt
    40981 gactacagag gccaaaataa gcccaatcat cacgcactta acgacaatat cggcgtactg
    41041 atacataccc cagacggaaa ggtccgtctg cattaaatta ttacccactg tgtatctcca
    41101 ggacgcaagt cacaaaatct gcgcataata atatcaaaac gacgtcgaat tgatagtcgt
    41161 tctcattact atttgcatac tgccgtacct ttgctttctt ttccttgcgt ttacgcagta
    41221 aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg
    41281 cgcccgcgaa tattcatgct agtttagaca tccagacgta taaaaacagg aatcccgaca
    41341 tggcggacaa aaagcttgat actcaactgg tgaatgcagg acgcagcaaa aaatacactc
    41401 ttggcgcggt aaatagcgtg attcagcgcg cttcttcgct ggtatttgag agtatggaag
    41461 ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt gttctatgga cggcgcggaa
    41521 cgttaaccca tttttcctta caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg
    41581 cgctatttcc ctgcggggcg gcagcggttg ctaattcgat tcttgctttt gtcgaacagg
    41641 gcgatcatgt gttgatgacc aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa
    41701 tcctcagcaa actgggcgta acgacatcgt ggtttgatcc gctgattggt gccgatatcg
    41761 ttaagcatct gcagccaaac actaaaatcg tgtttctgga atcgccgggt tccatcacta
    41821 tggaagtcca cgacgttccg gcgattgttg ccgccgtgcg cagtgtggtg ccggatgcca
    41881 tcattatgat cgacaacacc tgggcagccg gtgtgctgtt taaggcgctg gattttggca
    41941 tcgatgtttc tattcaggcc gccaccaaat atctggttgg gcattcagat gcgatgattg
    42001 gcactgccgt gtgcaatgcc cgttgctggg agcagctacg ggaaaatgcc tatctgatgg
    42061 gccagatggt cgatgccgat accgcctata taaccagccg tggcctgcgc acattaggtg
    42121 tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc cgaatggctg gcagaacatc
    42181 cgcaagttgc gcgagttaac caccctgccc tgcctggcag taaaggacac gaattctgga
    42241 aacgagactt tacaggcagc agcgggctat tttcctttgt gcttaagaaa aaactcaatg
    42301 atgaagagct ggcgaactat ctggataact tcagtttatt cagcatggcc tactcgtggg
    42361 gcgggtatga atcgttgatc ctggcaaatc aaccagaaca tatcgccgcc attcgcccaa
    42421 aaggcgagat cgattttagc gggaccttga ttcgcctgca tattggtctg gaagatgtcg
    42481 acgatctgat tgccgatctg gacgccggtt ttgcacggat tgtataacat tgccactttt
    42541 ggacaatttt gcagacattt tattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg
    42601 cgtcccggac gattcaggag tacaataggc agataaaggc ttaaacgctg ttccacagga
    42661 aagtccatgg ctgttattca agatatcatc gctgcgctct ggcaacacga ctttgccgcg
    42721 ctggcggatc ctcatattgt tagcgttgtt tactttgtca tgtttgccac gctgttttta
    42781 gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca gcttgttgat actggcaggc
    42841 gcattgattg cccaaggggt tatggatttt ctgcctacga ttgcgattct gaccgccgca
    42901 gcaagtctgg gctgctggct aagttatatt caggggagct ggttagggaa taccaaaacg
    42961 gtgaaaggct ggctggcaca gcttcctgct aaatatcacc agcgcgccac ctgcatgttt
    43021 gaccgccacg gtctgctggc gctgctggct ggacgttttc ttgcatttgt ccgtacgctg
    43081 ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg
    43141 ttaagtggat tgctgtgggt cagcgtggta accagttttg gctatgcctt aagtatgatt
    43201 ccgttcgtta aacgccatga agatcaggta atgacgttcc tgatgatcct gccaattgcc
    43261 ttgttaaccg ctggcttgtt aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac
    43321 gcctgacgat tttccccgtt cccggctgct gtaccgggaa cgtatttaat tcccctgcat
    43381 cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg aagtaacgtt taaactcacg
    43441 gctaaattgc gatgcgcttt catagccgac gcgcatcgct gctgcgctgg ccttcatgcc
    43501 gtcatggatg atcatcatcc gcgccttatg cagacggtaa ttcttcaaat actgcaacgg
    43561 cgaggtgctg gtgacagact taaaattatg gtggaacgcc gatacgctca tgttggcttc
    43621 tgccgccagt tgctcgacgc tcaggttttc ggtgtattta ttctcaattc gtttcagcac
    43681 gcggctaatc agactgaagt gagtctggcg actgaccagc gccagtaacg cgccgccgca
    43741 aggtccggtc agcacgtagt acagaatttc gcggatgatc tgtttgccga gaatacgcgc
    43801 atccagtggt cgctccatca cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga
    43861 taacgtggcg gagttaatcc cgctggctgc catcgacggc tggaaatgct catcttcgcc
    43921 aatgtccatc aacagttcct gtaactgcaa aatatcgaca ttgagacgca accctgccag
    43981 cggcacctct gacgtggcat aggtttcgca ctcaaacggc aacggcaccg tcagcagcag
    44041 gtattcattg gcatcataac gaaacacgcg ttcattgata taaccgattt tatgcccgga
    44101 aaagagaatt atgatgccag gctcgtacat caccggtgta cgtgcgaaag gcgtctcgcc
    44161 atacaacaaa cgcacatcgg gcaacagttc tgacaaacta ttttctttat ttttcagttt
    44221 attaacttta tccgccagca agcgacaaat ctcttcacgt ttcatatcgc gtaatttctt
    44281 aggaataatg cggcaatttg attgtgcgca attttgtagc atttctccag cactctggag
    44341 aaataggcaa gacattggca gaaatgagca ttgagagcca gggcgctggc gatcacaatg
    44401 aaaaacatca ggcagatcgt tctctgccct catattggcc cagcaaaggg agcaagtaat
    44461 gaacaacttt aatctgcaca ccccaacccg cattctgttt ggtaaaggcg caatcgctgg
    44521 tttacgcgaa caaattcctc acgatgctcg cgtattgatt acctacggtg gcggcagcgt
    44581 gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg aaaggcatgg acgtgctgga
    44641 atttggcggt attgagccaa acccggctta tgaaacgctg atgaacgccg tgaaactggt
    44701 tcgcgaacag aaagtgactt tcctgctggc ggttggcggc ggttctgtac tggacggcac
    44761 caaatttatc gccgcagcgg ctaactatcc ggaaaatatc gatccgtggc acattctgca
    44821 aacgggcggt aaagagatta aaagcgccat cccgatgggc tgtgtgctga cgctgccagc
    44881 aaccggttca gaatccaacg caggcgcggt gatctcccgt aaaaccacag gcgacaagca
    44941 ggcgttccat tctgcacatg ttcagccggt atttgccgtg ctcgatccgg tttataccta
    45001 caccctgccg ccgcgtcagg tggctaacgg cgtagtggac gcctttgtac acaccgtgga
    45061 acagtatgtt accaaaccgg ttgatgccaa aattcaggac cgtttcgcag aaggcatttt
    45121 gctgacgcta atcgaagatg gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg
    45181 cgccaacgtc atgtgggcgg cgactcaggc gctgaacggt ttgattggca ctggcgtacc
    45241 gcaggactgg gcaacgcata tgctgggcca cgaactgact gcgatgcacg gtctggatca
    45301 cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat gaaaaacgcg agaccaagcg
    45361 cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc actgaaggtt ccgacgatga
    45421 gcgtattgac gccgcgattg ccgcaacccg caatttcttt gagcaattag gcgtgccgac
    45481 ccacctctcc gactacggtc tggacggcag ctccatcccg gctttgctga aaaaactgga
    45541 agagcacggc atgacccaac tgggcgaaaa tcatgacatt acgttggatg tcagccgccg
    45601 tatatacgaa gccgcccgct aagcttttta cgcctcaaac tttcgttttc gggcatttcg
    45661 tccagactta agttcacaac acctcaccgg agcctgctcc ggtgagttca tataaaggag
    45721 gaacgtatgg ctaatccaac cgttattaag ctacaggatg gcaatgtcat gccccagctg
    45781 ggactgggcg tctggcaagc aagtaatgag gaagtaatca ccgccattca aaaagcgtta
    45841 gaagtgggtt atcgctcgat tgataccgcc gcggcctaca agaacgaaga aggtgtcggc
    45901 aaagccctga aaaatgcctc agtcaacaga gaagaactgt tcatcaccac taagctgtgg
    45961 aacgacgacc acaagcgccc ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt
    46021 gattatatcg acctctactt aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa
    46081 gcatggaaag gcatgatcga attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc
    46141 aacttccaga tccatcacct gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata
    46201 aaccagatcg aacttcatcc gctgatgcaa caacgccagc tacacgcctg gaacgcgaca
    46261 cacaaaatcc agaccgaatc ctggagccca ttagcgcaag gagggaaagg cgttttcgat
    46321 cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa ccccggcgca gattgttatc
    46381 cgctggcatc tggatagcgg cctggtggtg atcccgaaat cggtcacacc ttcacgtatt
    46441 gccgaaaact ttgatgtctg ggatttccgt ctcgacaaag acgaactcgg caaaattgca
    46501 aaactcgatc agggcaagcg tctcggtccc gatcctgacc agttcggcgg ctaacgtgca
    46561 aattctcccg gtggcggtaa tgttccgcta ccggactttt cagaaatcat ttattcccct
    46621 cgcgtcccgc ccgttgttac tcttccttgt tcaggaatgc caaatataag gacatcatca
    46681 tgcagagccg gaagctctta aaagaacaac tcatctatat ccgggataaa cgcaacggag
    46741 aggtgaaaaa cagatgaaaa taatacttct gtttttagca gccctggcaa gttttaccgt
    46801 acacgcgcag cctccttcac agaccgtaga acaaacagtc cggcagattt atcagaacta
    46861 taaatcagat gccagtacgc cttatttcgg tgaaaccgga gagcgggcga taacttcagc
    46921 gcgtattcaa caggcgctta ccctgaacga caatcttaca ctaccgggca atattggctg
    46981 gctggattat gatccggttt gtgattgtca ggattttggc gatctggtgt tagaaagcgt
    47041 agcgataacc caaactgacg tcgatcatgc cgatgccgtt gtgcgctttc gtatctttaa
    47101 agatgataaa gaaaagacca cacagacact gaaaatggtg gcggaaaatg gtcgttgggt
    47161 cattgatgat attgtcagca atcatggcag cgtcttacaa gcagttaata gcgagaatga
    47221 aaaaacgctg gccgctttag cttcattgca gaaagaacag ccagaatcct ttgttgctga
    47281 actctttgaa catattgctg attatagctg gccgtggacg tgggtggttt ccgactctta
    47341 ccgccaggcg gttaatgcct tctataaaac caccttcaag acggccaata atcccgatga
    47401 agatatgcaa atagaacggc aatttattta cgacaatccg atctgttttg gcgaagagtc
    47461 gctattttca cgcgttgatg aaattcgagt cctggagaaa accgccgatt ccgcccgcat
    47521 tcatgttcgt tttacgctga ccaatggcaa caatgaagag caagagctgg ttttacagcg
    47581 gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg aacagcggca gcctacttaa
    47641 gcagattgag gcaaaaaccg ccgccagatt aaagcaatga gctgaattaa ataacaatta
    47701 gccggaacaa taaataaaag ggaacactat atgaaaacga ttttcaccgt gggagctgtt
    47761 gttctggcaa cctgcttgct cagtggctgc gtcaatgagc aaaaggtcaa tcaactggcg
    47821 agcaatgtac aaacattaaa tgccaaaatc gcccggcttg agcaggatat gaaagcacta
    47881 cgcccacaaa tctatgctgc caaatccgaa gctaacagag ccaatacgcg tcttgatgca
    47941 caggactatt ttgattgcct gcgctgcttg cgtatgtacg cagaatgata aaaaaatccc
    48001 cggcagcatg tcagttgccg gggatttttt ttaacgtcca accgccgctt tagggcgttt
    48061 cttcgcacca gcattcaccg gacgagattg cgtagacgac gctttttttg ccgtagcagg
    48121 cgtctgacgc tgagtcgcca tcggcgtatg tttagtcaac gccggacggg tattgcggtt
    48181 ctggcgacga gcttcacgca tctcttcaat ggttggcgca ggcactaagc aatcgcgacg
    48241 gctgccaatc agatgctttt tgcccatcgc ttccagcgcc tgacggatta acggccagtt
    48301 tgccggatcg tggtaacgca acaacgcttt atgcaaacga cgctgtttgt cgcccttcgg
    48361 tacgaagacg ccttcgctct tataaccaat cttcgccagc ggttttttcc cggtgtaata
    48421 catggtggtt gagttcgcca gcggcgatgg gtagaagttc tgtacctggt cgagacggaa
    48481 acgatgcttt ttcagccaca gcgccaaatt caccatatct tcatcacgcg taccggggtg
    48541 cgcagagatg aaatacggga tcagatactg ctctttacct gcctgtttcg agtaggtatc
    48601 gaacagctct ttaaagcggt catagctgcc catgcccggc ttcatcatct tcgacaacgg
    48661 cccttcttcg gtatgttccg gagcaatctt cagataaccg ccgacgtgat gggtcgccaa
    48721 ctctttgata tagcgcggat cttccacggc gatgtcataa cgtacgcctg aggcgataag
    48781 gattttttta atgcctttca gatcacgcgc acggcgatag aggttgatcg tcggttcgtg
    48841 gttagtgtcc atgtgcggac aaatatccgg ataaacgcac gacaaacggc gacaagtttg
    48901 ttcagcgcgt ggcgatttgc agcgcaacat atacatgttg gcagttggcc caccgagatc
    48961 ggaaatcacg cccgtaaaac ctggaacggt gtcgcggatc gcttcgatct cattaatgat
    49021 cgaatcttcc gaacggctct gaataatgcg cccttcgtgc tcggtaatag aacagaaaga
    49081 acagccgcca aagcagccgc gcataatgtt gaccgaaaaa cggatcattt cgtaagccgg
    49141 aatacgggca ttgccatagg ccggatgtgg cacgcgcttg tacggcagcg caaaaacgct
    49201 gtccatctct tcggtagaaa gcgggatagc aggcgggttg atccacacat agcggtcacc
    49261 gtgtttttgc atcaatgcgc gggcgcagcc tgggttggtt tcgtggtgca gaatgcgcga
    49321 agcatgggcg tacagcactt tatcgccctt cactttctcg aaagaaggca gcaatacgta
    49381 ggttttttcc cacggtttct ggcgcggtgg ctgcacgatt acagctttcg cttcctgctt
    49441 tttcggcgca acaggtttgt tatccgcaca cggcaaatct tcgccatacg gatgcgggat
    49501 tggggcgatt ttccctgggg tatcaagacg ggtggaatcc acaccgctcc agccaggcag
    49561 cgcctctttt acgataattg cggtattacg cacatcgcgg atttcactaa tcggctcgcc
    49621 catcgccaga cgatgcacta cttccaccag cggacgttca ccgttaccaa acatcagcat
    49681 gtcggctttc gaatctacca gaacggaacg gcgcacggta tcggaccagt aatcataatg
    49741 cgcggtacgg cgcaggctag cctcaatacc gccgaggatc accggcacat ctttccacgc
    49801 ctctttacaa cgctgggtat aaaccagcgt ggcgcgatcc ggtcgcttac ccgcgacatt
    49861 atctggcgtg taggcatcat catgacgtaa acggcgatcg gcggtatagc ggttgatcat
    49921 cgaatccatg ttgccagcag taacaccaaa aaacagattc ggtttaccca gacgcataaa
    49981 gtcgtctttg ctgctccagt ccggctgggc gatgatcccg acgcgaaagc cctgcgcttc
    50041 cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga tcgacatacg cgtcgccagt
    50101 aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc atctcttcac gagacatcgg
    50161 caaaaacggt gccggtccaa aacaggcggc ccagtactgc ggccaggaga acaggtcgcg
    50221 atccggttgg atcagggaga tagagctcat tttgcttcca gaaatgataa aaaaataatc
    50281 aaaggccggg gattataagc cggaacgaaa gagaaatcga aaggtattcc atactcgccc
    50341 tcctcgggcg agtatgaaga ttacggtacc ggattgacca acagttgccc aatcgagcca
    50401 cggtccgcca tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc
    50461 tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc tttccagcct
    50521 ctgtcttccg ggaacggcat cgcgccattc acgttacgga tctggaacat tacgccttca
    50581 atatggaacg cttgcggctc gtccgcgcgt accgtccagc gttcccacgt tccttgctgc
    50641 gcggtgacat caatacggtt gacgtcccac agctgtccat taatacccgg gtcatcaccc
    50701 agactgatat cgcgactgcg aattggcgaa ccagccatga tttcagttgg cagcaagcgc
    50761 atcggaagac tgtctgtgac cagcggcaga aggccggttg ggcgcagcgt tagcaccaag
    50821 gtagaaacca gaatactgga tggctcaaag aagccgcgaa tacgatcaac aatgctcgcc
    50881 gcttcaccac aggtgatcga cacttcatcg ccgttgctca tatccaccag aatctcgcgg
    50941 cgctcgcccg gtgccagcga aagttgcttc accgatacag gagcaggcag gaatccctga
    51001 tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct gtagttgata gcgacgagag
    51061 ttcgacgcgt tcagcagtcg caagcgcacc cagccacgcg agacttcaac gtacgggctt
    51121 tgtacaccgt taaccagcag cgtatcacca acaaagccgc cgcttcccgg ttcgttgtat
    51181 tctggcgtac caaagttatc cagccgttta tcctggataa tgaccggaaa atcatccaca
    51241 ccataatggt tggggatagg cagcgacttg ctgacttcat cttccaccag ccacattccg
    51301 gcaaggccgt tatagacctg ctgagccgtg cggttgggag tattggcgtg ataccacaga
    51361 gtagctgcgt tctggcgaat aggcagtacg ggtgcccagt cagcgtttgg cgacatcatc
    51421 cgtgccggac cgcccatcag cgggcctggt acctgtagcc cggccaccgt cattgagaca
    51481 ttttctgtca ggcggttgct gtaaataagc ttaacatcgt cgcccttcca gacgcggata
    51541 gtcggcccca ggtaacgacc attgattccc cagaccgacg cgcgtgtccc tggcgtaaat
    51601 gaccagtgcg cacgttgtac agtcataaac agcggttgcc cacggcgaga ttcaagtagc
    51661 ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct
    51721 gcacaaagtg caatccccga tgcctgaatg aactgacgcc gactgagtga catatttgct
    51781 ccatgtaaaa ctggctaaag taataccgtg caggaaagtg ttccccgcaa aaatacagat
    51841 tgccgactta aacttttccg gcggcttcgc gttctgcgac ttctttatcg agctcggcga
    51901 ttttttgttc cattatcgaa cgacaatggg cagccagctc acgaacctga tctttgccat
    51961 actgactgac gtcaattggc ggcagcattt cgacaatcac cagaccgttg tgcagtcgat
    52021 taagattaat cttattcgaa gttgtagaga cgcacacggg aataatcggg acgcccgccg
    52081 caattgccgc gtgaaatgct ccagtcttga acggtagcag gccgcgacca cggctgcggg
    52141 ttccttccgg gaacatccag atggaaatac ggcgtttttt gaaatgattc acgacttccg
    52201 caatggtgcc gtgagcttta gtgcgattgt ttctgtcgat caataagttg ccggttaacc
    52261 agtacaactg cccgaagaag gggatccaca gcaagctctt tttacctacc gtcaccgtcg
    52321 gcggttgcac aatgttcgat gctgtcacca tgtcatagtt gttctggtgg ttagcgatat
    52381 agatagcatt gccgtagttt tcagcatctg caggtttgcg gcactcaact ttcaggccga
    52441 acagtggcgc aagacggcca aacatatgcc caaaggtggc cacatgtttc gggttacgcg
    52501 ggctgaaaag gcagtaaatg gagccgaata cacagactaa gatgctgtaa atcacggtaa
    52561 taataagacg aaagatatat agcataacac cctctgaagg tctgagagac tggcattgta
    52621 cgtcacctga aatcaggtta gggcattgtt aaaagccgct ctctggaaaa gcgtattgtt
    52681 aatcgcaacg catgaataaa caacggtttc aagggaattt ttatcgtttc cgggggttga
    52741 atacgctgcc ggatgacgac ttaacgtttc atccggcgtt ccttgctagc gggaggaaac
    52801 agcgccctcc ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc
    52861 gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc
    52921 gccagtgact ttctgtagct cttccggacg cagtttaatt ttgcgtttcc caacatgaat
    52981 ggtcagcgtg ctttgcggcg gcagaacgta cagttgcgcc agaccatcct cgccacgcgc
    53041 ggcttctgcc gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag
    53101 atcacttacc gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga
    53161 agcatcttca atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc
    53221 cttacccgca cggttacgcg ctaccagatc gttaaaggtg cagacgaaac cgtaacccgc
    53281 atcggaagcc atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc
    53341 cccaggcggc aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag
    53401 cgtaatcggg tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt
    53461 actcttacct ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc
    53521 gtcgatatca tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac
    53581 aggttcagac ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa
    53641 cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag
    53701 gttattcatt ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct ctttttccag
    53761 ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag
    53821 ttcgaggatc gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt
    53881 cggttcatct tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag
    53941 caaaccttcc aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg
    54001 gcgcacggta tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac
    54061 cgccggacga ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc
    54121 ggtggtagcg aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac
    54181 aatcaccaga cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg
    54241 cagctttttg ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg
    54301 atgcggtaat gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg
    54361 cactgaacca cgtccgttct cgtagatttt acgaatctcg gcgcgcgaag tgataatttc
    54421 cgcttcagtc ggataatccg gcccctgcac gatatccagc agctgatcga gcgtagtttt
    54481 cggctggtcg attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat
    54541 atcggtcgcc atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg
    54601 ggcaggtagc attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt
    54661 cccctgcccc agctcgctca atagcagctc ggaatatttc gacaaccggg attcggtgta
    54721 acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac
    54781 cagcggataa cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcgct
    54841 atcgccgtgc ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa
    54901 tttggcgctg gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg
    54961 tttcagacca tcaccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt
    55021 taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa
    55081 ttctgattcc tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca
    55141 ttatctgccg cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga
    55201 cggcgataaa ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca
    55261 tagagggcag cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg
    55321 cgattcgaga agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac
    55381 tgtaagtata cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc
    55441 cataagtgcg gcttgctcat ggatatcccc gagacggctt acggcgcacc ggataataaa
    55501 tttcgcgtat acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg
    55561 tgacattgtt cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc
    55621 catcacgcca acggcacata aacatccgtt tccagtcgct gctctgtcac cccttcggca
    55681 agattggtgt agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc
    55741 cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg
    55801 gccacggcat aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga
    55861 atcggttcgc taacgctgcc gcagatatca aagcgaaacg cttccggtgc ggtggtggca
    55921 ggggcgtccc aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct
    55981 ttacgccaca tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc
    56041 ggcaacatcg ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt
    56101 gtcattttgc ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta
    56161 cggatttact ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac
    56221 ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg
    56281 atcgatatca acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat
    56341 gcgttcaacg atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct
    56401 ttctacagtc gttacgctgc cgttcggccc ctgaaatcct ccggcctgac tttgcgttac
    56461 cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat
    56521 tactgcgatt actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg
    56581 agcatttaac aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg
    56641 tacagagcgc gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa
    56701 aatgcgaatt ttactgatag aagatgacat gctgattggt gacggcatca aaactggcct
    56761 tagtaaaatg ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct
    56821 ttatagcgca ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg
    56881 cgatattttg cgcgaatggc gagagaaagg tcagcgtgag ccggtactga acctgaccgc
    56941 gcgtgatgcg ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg
    57001 taaacctttt gcgttgatag aagtcgccgc caagctggag gctctgatgc gccgaaccaa
    57061 cggccaggcc agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat
    57121 cgccacgctg gctggcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt
    57181 actgatgcgt aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac
    57241 ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa
    57301 actcggtagt gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg
    57361 aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc
    57421 tcggtgacct ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat
    57481 gaattgttcg acacccaact gatgctgttt gccaaacggt taagtacgct cgatctcaac
    57541 gaaatcaacg cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt
    57601 gatgacgatg cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat
    57661 ggcgataacg gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa
    57721 ctggtcggtg aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat
    57781 cgcatcgttg ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc
    57841 gggcaattga tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta
    57901 ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct
    57961 gactcggaaa aaccactaaa cgcgactggc gtacccagcg aagtgcggcc actggttgag
    58021 tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc
    58081 tccgatgcag ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt
    58141 gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag cactgctcca attacattcc
    58201 gggatcgatc gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca
    58261 ctggataacc ttcaggacgt cgcggagatc ccgcttgaag atctcctaca atcgtcggtg
    58321 atggatattt accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgcc
    58381 cacggcatca aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg
    58441 gataacggcc ccggtgtgac accagaggca ctggcgcgaa ttggcgaacg cttctatcgc
    58501 ccacccggac aaaccgctac cggcagtgga cttgggctat cgattgtcca gcgaatcgcc
    58561 aagctgcatg acatgaatgt tgagtttggg aatgcggaac aaggtggatt tgaggcgaag
    58621 gtaagctggt aacttctctg tctatgagag ccgttaaaac gactctcata gattttacta
    58681 atagcaaaat ataaaccgtc cccaaaaaag ccaccaacca caaaccagac aaataacgag
    58741 cgagaataca tatagccaaa actcgaacat ttcccgcata aagagttttc ttaagataag
    58801 aataataagt ggcgtaagaa gaaaaaatgc tgcaatcatt gcttgcaata tacgatcttc
    58861 gcctgaaacg gaaggtatac tgcaaatatt gcctcccgac tcaagcatcc agtcatactt
    58921 attgaaagca aaaaaagaat ctattaacca ataagtaagc attatatata gcaatgcttc
    58981 aaatattgtt ttaattttct gctttaacat atatcagtac gctcataaaa atccatttat
    59041 aattatcaac atgacacgtt gatattttca tcttttactt attcaaagcc atattttcaa
    59101 tccccaaaaa aaccgaacca aacgtgtaaa ttattccggt gcgagtaaga attatgagga
    59161 atggctatca gtattgtcat tttcagaaaa tatttatcct gcatcggtga gtcagagcta
    59221 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt cacagtaccg
    59281 ctctgtgacg tactatagtc gacaaacgtc tcaccttgag attaaaaaat gagcaacatc
    59341 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
    59401 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc
    59461 gacagcgact acaatgtcaa agcggaagta caaaactttc tctgggctga tgtggtgatc
    59521 tggcagatgc caggctggtg gatgggcgcg ccgtggacag tgaaaaaata tattgatgat
    59581 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg
    59641 tcgaaaaaat acggttccgg cagcctggta cagggcaaaa aatatatgct ttctctaacc
    59701 tggaacgcac caatggaagc cttcaccgaa aaagatcagt tcttccacgg cgttggcgtt
    59761 gacggtgtgt atctgccgtt ccataaagca aaccaattcc tcggtatgga accgctgccg
    59821 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
    59881 cgcaagcatc ttgtggaaat ttttggttaa ctagagctca ggctttagaa ggagttaacc
    59941 atgcttaccg taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcggta
    60001 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
    60061 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc
    60121 gtgatgattg agcagtggga aagcatcgcg caccttgaag cgcatctgca aaccccgcac
    60181 atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg agatgaatat ccgtattctg
    60241 cagccaggga tttaatcctg ccttgtttgc ccggccatcc tgaccgggca atgttctttc
    60301 ctttaaacct caatctccgc catgtcgcct ttctcttgca accagttgcg gcgatcttcc
    60361 gagcgtttct tcgccagcag catatccatc atcgcgtcag tacgctgatc gtcttcatca
    60421 tcgatagtca actgcaccag acggcgagtg ttcggatcaa gcgtggtttc acgcaattgc
    60481 atcgggttca tttcccccag acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt
    60541 tttaattgct caagtacgcc ctctttctct tcttccgtca gcgcgtaata aacctctttc
    60601 ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac
    60661 gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg caatgtgcag accatcagag
    60721 tccgcatccg cgaggataca gattttgcca taacgaagct ggctcagatc gtcgctgtca
    60781 ggatcgatac cgatcgctac cgaaatatca tgcacttcct gcgaggccag cacttcgtcg
    60841 gaagaaactt cccaggtgtt aaggatctta cctttcagcg gcatgatcgc ctgatattca
    60901 cgatcgcgcg cctgcttggc agatccgcct gcggagtcac cttccactag gaacagttcg
    60961 gtacggttaa ggccctgcgc ggtacaatca gccagtttgc caggcagcgc cgggccgctg
    61021 gtcagctttt tacgcaccac ttttttagcc gcacgcatac gacgctgggc gctggaaatc
    61081 gccatctctg ccagcaactc cgccgcctga acgttctggt tcagccacag gatgaaggca
    61141 tctttcacca cgcccgaaac gaatgccgca cactgacgcg aagagagacg ctctttggtc
    61201 tgcccggcaa actgcggatc ctgcatttta accgacagaa cataggcgca gcgatcccag
    61261 atatcttccg ccgacagctt cacgccgcgc ggcagaatgt tgcggtattc acagaactca
    61321 cgcatcgcgt ccaacaggcc ctgacgcaga ccattaacat gggtaccgcc ctgcatcgtt
    61381 gggataaggt tgacgtagct ttcggtcagc agttcaccgc cttccggcag ccacagtagc
    61441 gcccagtcca cagcttcagt atcgccagcg aaattaccga taaacggttt ttctggcagc
    61501 gtcggtaaac cgtttaccgc ttccgccagg taatcattca gaccgtcctg atagcaccag
    61561 cgctgttcgg tgttgttgat ctcatcttta aaggtaatct caacgccggg gcacagtact
    61621 gctttggcct tcagcacatg cgtcaggcgt gaaacagaaa aacgcgggct gtcaaagaag
    61681 gtttcatccg gccagaagtg cacgctggta ccggtattgc gtttaccgca agtgccgaca
    61741 acctgtaaat cctgcacctt ttcgccattt tcaaaggcga tgttataaac ctgaccatcg
    61801 cggcgcacgt taacttctac gcgcttcgac aaggcgttaa ctaccgaaat ccccacgcca
    61861 tgcaggccgc cagagaactg gtaatttttg ttagagaatt taccgcctgc atgcagacgg
    61921 caaagaatca gttcaaccgc cggtactcct tcttccgggt gaatatccac cggcatcccg
    61981 cgcccatcgt caatcacttc taacgactgg tcagcatgta agattacatc cacgcgtttt
    62041 gcgtgacccg ccagtgcttc atcgacactg ttatcaataa cttcttgccc caaatggtta
    62101 gggcgagtgg tatcggtata catccccgga cggcggcgaa ccggctcaag cccggtgagt
    62161 acctcaatgg catcagcgtt ataagtttgc gtcatggttt aagttagcaa ttcgagttga
    62221 tcgtcagaga tggtgcagac caagaaaatc gacgatcggg ttgaaataat cttcgaagcc
    62281 cgtgaatgcg tggttgccgc cttctatgac agtctggcgg caggaagcgt agtacgccac
    62341 cgcctggcgg taatccagca cttcatctcc cgtctgttgc agcagccaga tcaaatccgg
    62401 cgcttccagc gggtcaatct gcatgacttt gagatcgtaa atatggcgtg actctagcac
    62461 atattgctgc ccggtgtagg ggttctcgtt ctgaccgaga tagtccgtca gcaattcaaa
    62521 cgggcgtacc gccgggttta ccaccactgc gggcagcata aaacattgcg acaaccaggt
    62581 ggcgtaatat ccccccagtg acgaaccgac aatacccagc gaatcgccgc catgttccag
    62641 gacaatggat tccagcagtt ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat
    62701 catctcaacg tcagggtgat gttccgccag ccagtttttt aacaagctcg cttttgcaga
    62761 gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta gacatcagta gccttctgaa
    62821 gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca
    62881 tcagcatgta actcgagagt acgccagccg ggcgcgatag tatccagcgt aaagttggaa
    62941 cagtgcggct taaactgcac acaggtcgac ggcgttgcca gcaggcggcg accgttccag
    63001 tcgagatcca gctcctgatg aatatgaccg cacagcaagt atttgacgtg cggaaacttc
    63061 gccagcacgg tatccagttc gcccgcgtta cgcagactgt gttgatcgag ccaactacaa
    63121 cccgcaggta gcggatgatg atgcagcagc agcaacgtat ggcgttctgg cgcatcggcc
    63181 agtttacgtt ccagccactc aagctgaaac tcgctcagct caccgtgcgg cacgccaaac
    63241 acctggctat ccagcaacag gatttgccat tgctcaccaa taaacacccg cttcgccggg
    63301 gagatacccg catcctgtaa cgcgctgtac atcgcgggct ggaaatcgtg gttgcccggc
    63361 agccagacgc agggcgcacg aaaacttgcg atgccttcag cgaaatgctg ataggccgca
    63421 gaggattgat cctgcgctaa atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt
    63481 ggacgaatcg cctccagcac cgcctggtag ctttcccagg tgtttacccc taacagggct
    63541 tcgtgctttt gtgcaaacag gtgagtatcg gtaatttgta aaatccttcc tctggcctca
    63601 ccagccagag gaagggttaa caggctttcc aaatggtgtc cttaggtttc acgacgctag
    63661 taaaccggaa tcgccatcgc tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac
    63721 tggttaattt gatgcttttc gtcgcgttga tgcaactttt tattaggata atcataccgc
    63781 gctttgaagc gaaaaatctg ctggcttgaa cacacttcag ccaccatcgc gtcatgatac
    63841 agacgcaccg tcattgacgg aaggctccag taactgatcg cgggcgcagt ctgttctatt
    63901 gtcactaggg tagtgtatcg ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc
    63961 acctgatagc ttacagtttc gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt
    64021 gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt
    64081 tatgcccact cattttttaa cgcttgatga tgcagctgca gccattgcaa agcgatgacc
    64141 gacgctgcgt tgtcgatttt cccctcttct acccactggt atgcctgttc ccggcttacc
    64201 acatgaacgc gaatatcttc gttttcatca gccagaccat gaataccgct tgcggtcgtg
    64261 gcgtccactt cgcccaccat aattgacgaa cgctcactgg tgccccccgg gcttgccagg
    64321 aaacttaaca ccggtttggt ccgtttgact atcagtcccg cctcttcaat cgcttcgcga
    64381 cgggcaacat cttccacact ttcaccctct tcaatcatcc cggcaaccat ctccagtagc
    64441 cagggggttt cgctggtgtc gtacgcggca atccgaatct gctcaatcag cacaacttca
    64501 tcacgcactg ggtcaaaggg tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc
    64561 cgtacctcat gactcatttg cccgttgaat agacgatgac gaaatctata aagatctaat
    64621 gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt ctacatcgtt tttgccaaat
    64681 gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc tagcactaat agtgaattaa
    64741 atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc atatgttaaa ttgaggcaca
    64801 ttaacgccct atggcacgta acgccaacct tttgcggtag cggcttctgc tagaatccgc
    64861 aataatttta cagtttgatc gcgctaaata ctgcttcacc acaaggaatg caaatgaaga
    64921 aattgctccc cattcttatc ggcctgagcc tttctgggtt cagttcgttg agccaggccg
    64981 agaacctgat gcaagtttat cagcaagcac gccttagtaa cccggaattg cgtaagtctg
    65041 ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac
    65101 agctaggttt aggtgcagat tacacctata gcaacggcta ccgcgacgcg aacggcatca
    65161 actcgaacgc gaccagtgcg tccctgcagt taactcaatc catttttgat atgtcgaaat
    65221 ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca ggacgtcaca tatcagaccg
    65281 atcagcaaac cttgatcctc aacaccgcga ccgcttattt caacgtgttg aatgctattg
    65341 acgttctttc ctatacacag gcacaaaaag aagcgatcta ccgtcaatta gatcaaacca
    65401 cccaacgttt taacgtgggc ctggtagcga tcaccgacgt gcagaacgcc cgcgcgcagt
    65461 acgataccgt gctggcgaac gaagtgaccg cacgtaataa ccttgataac gcggtagagc
    65521 agctgcgcca gatcaccggt aactactatc cggaactggc ggcgctgaat gtcgaaaact
    65581 ttaaaaccga caaaccacag ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc
    65641 tgtcgctgtt acaggcacgc ttgagccagg acctggagcg cgagcaaatt cgccaggcgc
    65701 aggatggtca cttaccgact ctggatttaa cggcttctac cgggatttct gacacctctt
    65761 atagcggttc gaaaacccgt ggtgccgctg gtacccagta tgacgatagc aatatgggcc
    65821 agaacaaagt tggcctgagc ttctcgctgc cgatttatca gggcggaatg gttaactcgc
    65881 aggtgaaaca ggcacagtac aactttgttg gtgccagtga gcaactggaa agcgcacatc
    65941 gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat taatgcttct atcagtagta
    66001 ttaacgccta caaacaagcc gtagtttccg cgcaaagctc attagacgcg atggaagcgg
    66061 gctactcggt cggtacgcgt accattgttg atgtgttgga tgcgaccacc acgctgtaca
    66121 acgccaagca agagctggcg aatgcgcgtt ataactacct gattaatcag ctgaatatta
    66181 agtcagccct gggtacgttg aacgagcagg atttgctggc actgaacaat gcgctgagca
    66241 aaccggtttc cactaatccg gaaaacgttg ccccgcaaac gccggaacag aatgctattg
    66301 ctgatggtta tgcgcctgat agcccggcac ccgtcgttca gcaaacatcc gcacgcacta
    66361 ccaccagtaa cggtcataac cctttccgta actgatgacg acgacggggc ttcggccccg
    66421 tctgaacgta aggcaacgta aagatacggg ttatctgccg cattcttccc ccttctcgct
    66481 tcaatttcga ccagccatcc tctattctga tgggtattta ccactggtcc cggaagacaa
    66541 aaatgaaacg gacaaaatcc atacgccacg catcgttccg caaaaactgg agcgcacgcc
    66601 atctgacacc agtcgctctc gcggttgcca ctgtttttat gctggctggc tgtgaaaaga
    66661 gtgatgaaac agtgtctctc tatcaaaatg ctgacgactg ttcagctgca aacccaggca
    66721 aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc
    66781 cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg tgaaggtcag tgtcagcaag
    66841 caccagccca ggctggcatg gcaccagaaa accaggcgca ggctcagcaa tccagcggga
    66901 gtttctggat gccgctgatg gccggttaca tgatggggcg tctgatgggc ggcggcgcgg
    66961 gatttgcaca gcagccgctg ttctcctcga aaaacccggc cagtccggct tacggtaaat
    67021 ataccgacgc gacgggtaaa aactatggcg cagcccagcc aggccgcacc atgaccgtac
    67081 cgaagacggc aatggcacca aaaccggcga ccaccactac cgttacccgt ggcggttttg
    67141 gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc aaccggcacc tcttctcgtt
    67201 caatgggtgg ctgataccga tggaaagagt cagtattacc gagcgcccgg actggcgtga
    67261 gaaagcccac gaatacggtt tcaattttca caccatgtac ggcgagccgt actggtgtga
    67321 agatgcttac tacaagttga ccctcgccca ggttgaaaag ctggaagaag tcaccgccga
    67381 actgcaccag atgtgcctga aagtggtgga aaaagtgatc gccagcgatg agctgatgac
    67441 caaattccgc ataccaaaac acacctggag ttttgtgcgt caatcatggc tgactcacca
    67501 gccatcgctt tattcgcgtc ttgatctggc gtgggatggc actggcgaac ctaaacttct
    67561 ggaaaataac gccgatacgc caacgtcact atacgaggcg gcgttctttc agtggatctg
    67621 gctggaagat cagcttaacg ccggtaactt gtcggagggc agcgaccagt ttaacagtct
    67681 gcaagaaaaa ctgatcgatc gcttcgttga gctgcgtgaa cagtatggct tccagttgct
    67741 gcatctcacc tgctgtcgcg acacggtgga agatcgcgga accattcagt atttgcagga
    67801 ctgcgcaacc gaagctgaaa tcgccaccga gttcctctac atcgacgaga taggtttagg
    67861 cgaaaaaggc cagttcacgg atttacagga tcaggtgatt tctaacctgt tcaagcttta
    67921 tccgtgggaa tttatgctgc gcgaaatgtt ctccaccaag ctggaagatg ctggcgtacg
    67981 ctggctggaa ccggcgtgga agagcattat ctccaacaag gcacttctac cgctactgtg
    68041 ggagatgttc ccgaatcacc cgaacctgct gcccgcttat tttgcggaag atgatcatcc
    68101 gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat
    68161 cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg tatggcgaag aagggatgat
    68221 tgttcagcaa ttccacccgt taccgaaatt cggcgacagc tatatgctga ttggtagctg
    68281 gctggtgaac gatcaacctg ccggaattgg cattcgtgaa gaccgtgcgt tgatcaccca
    68341 ggatatgtct cggttttatc cacatatttt tgttgaataa gccacgatac cggatggcac
    68401 tcgccatccg gtaattgtta gcctatctgc accgacagca tacttaaact gcccatttct
    68461 ataccatcga ccggaatggt aattggctcc tgcccatccc acacacctaa cacatacaac
    68521 aacggcagat agtgctctgg cgttgggttc gataacgcgc caccttcatg gtcgagataa
    68581 ttcaccagag gatgctgctc taccggccct tgccacgtca gattcgcttt cacatactca
    68641 ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt gccacttcac tgtgcgcagg
    68701 ttatgcacca cgttaccgct ggcgaccaac attattcctt catcacgcag cgccgcgagt
    68761 ttgcgcccca tttcgaaatg ccaggcgaca ggtttgctac tgtcgatact caactgcacc
    68821 atagggatat cggcgtcagg atacatctta atcagcacgc cccacgagcc gtggtcaaag
    68881 ccccaggctt ctttatccag cgtcaccggg atcggcgcta acagctcaac cagacgctgt
    68941 gccagcgcag gcgaacccgg agcaggataa tgcgtatcgt acagcgcctg cgggaagcca
    69001 ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac
    69061 cagtgagccg aaaccaccac aatcgcttgc gggcgtggca atgtcatccc caacttctgc
    69121 cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg acctaaaaac
    69181 aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt cattttgata
    69241 tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg atgatcatca
    69301 gttattttga cgatctgcct gaaggtgaag atttataagg agttgtcgat gtcagtacct
    69361 ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct cggcgttctc
    69421 ggacaaaaac cctcgaaccg cttactggcg ttttcgctag gttttgcggc ggggatcatg
    69481 ttgctcatct cattaatgga aatgcttcct gccgcactag cagctgaagg aatgtcgccg
    69541 gtgttgggtt atggaatgtt tatcttcggt ctgcttggct actttggcct ggaccgcatg
    69601 ttgccgcatg cgcatccgca ggatttaatg caaaaatcgg tgcagctgtt gccaaaatcg
    69661 atcaagcgca cagccattct gctcactctc ggcatcagtc tgcataactt cccggaaggg
    69721 attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggcat cgcactggcc
    69781 gtcgcgttgc acaatatccc tgaaggtctg gcagtggcag gcccggttta tgcggcaacg
    69841 gggtctaaac gcaccgcgat tctgtgggcg gggatttctg gactagcaga aattcttggt
    69901 ggtgtgctgg cgtggttaat cctcggtagc atgatttccc cggtggtcat ggcggcaatc
    69961 atggcggcgg ttgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc
    70021 aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg
    70081 ggattcagtt tagtgctgct acaaacggcg ggaattggtt aaaaacaaca tcagaagttg
    70141 tccagcacat ggacaacttc tgccagcttc acgaaagcct tattctacct tttcggctgt
    70201 ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg
    70261 gcgaaacgcc cgacaataac gtttcaaatg caggaaataa cccaccacaa ccagagcaat
    70321 caatacaatc cagtaccagg cgataaacat ccttttaccc ttaatattta acaatactaa
    70381 acatttagcg cataaatttc acatatcctt tttcggatat attcatatgg tcgcataata
    70441 acggaacaat tatatgattt tgatcataat gaaaaagaat gcctgatatt aatgaaattt
    70501 tctcatagaa agagaagcgg aattaacatc ttgcatagca gggcaaaata aaaaccgaag
    70561 ccagctacaa cttcggtttc ttgattaagg cagtaattaa gcagcggttt tcagctggct
    70621 ttacgctcat gtgcctgacg gtatgccacc aggtcttcaa tagtcacgag cgccatattg
    70681 tgtttattgg caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc
    70741 agctcacaca gcacaccagc cggtttaaag cctgccagcg tcatcaaatc aatagtcgct
    70801 tcagtatgac cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg
    70861 cccgggcgat tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta
    70921 atacgatcag cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta
    70981 aaaccggtgc cataggcgct ggtgttattt tctaccatca ttggcagatc aagttgttta
    71041 cggcgatctt cagtgatgca caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc
    71101 atctgctcaa cggtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct
    71161 tcatcatcaa gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca
    71221 cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac
    71281 taaaattatg gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa
    71341 cgtgagcggg tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg
    71401 gccccggaat tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct
    71461 ttcaactgag ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat
    71521 tcactataac gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg
    71581 acttaccctt atcgcactac aatggtactc aacacctatc agtacaggga aactacaatg
    71641 attgacccga aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga
    71701 atcagggagt tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agctcagctg
    71761 acgcgcctcg accttgtaag ccgtgaagag ttcgacgtcc aagcgcaagt cctgt
//
LOCUS       DAJTNW010000007        70707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNW010000007 DAJTNW010000000
VERSION     DAJTNW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..795
                     /locus_tag="OO179_000482"
     CDS             <1..795
                     /locus_tag="OO179_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5530666.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO179_000483"
     CDS             806..1897
                     /locus_tag="OO179_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5530667.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTQFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO179_000484"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO179_000484"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5530668.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO179_000485"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO179_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5530669.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO179_000486"
     CDS             4859..5254
                     /locus_tag="OO179_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5530670.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO179_000487"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO179_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5530671.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO179_000488"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO179_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5530672.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO179_000489"
     CDS             complement(6563..7603)
                     /locus_tag="OO179_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5530673.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO179_000490"
     CDS             complement(7676..8311)
                     /locus_tag="OO179_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5530674.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO179_000491"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO179_000491"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5530675.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO179_000492"
     CDS             complement(8937..9380)
                     /locus_tag="OO179_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5530676.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO179_000493"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO179_000493"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5530677.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO179_000494"
     CDS             complement(9720..10223)
                     /locus_tag="OO179_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5530678.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO179_000495"
     CDS             complement(10217..10741)
                     /locus_tag="OO179_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5530679.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO179_000496"
     CDS             10950..11945
                     /locus_tag="OO179_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5530680.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     HYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO179_000497"
     CDS             11954..12832
                     /locus_tag="OO179_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5530681.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO179_000498"
     CDS             12913..13920
                     /locus_tag="OO179_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5530682.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO179_000499"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO179_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5530683.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO179_000500"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO179_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5530684.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO179_000501"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO179_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5530685.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO179_000502"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO179_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5530686.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO179_000503"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO179_000503"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5530687.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO179_000504"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO179_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5530688.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO179_000505"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO179_000505"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5530689.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO179_000506"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO179_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5530690.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO179_000507"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO179_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5530691.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO179_000508"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO179_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5530692.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO179_000509"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO179_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5530693.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OO179_000510"
     tRNA            complement(27654..27730)
                     /locus_tag="OO179_000510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OO179_000511"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OO179_000511"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5530694.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OO179_000512"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OO179_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OO179_000513"
     tRNA            complement(31514..31600)
                     /locus_tag="OO179_000513"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO179_000514"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO179_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5530695.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO179_000515"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO179_000515"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5530696.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO179_000516"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO179_000516"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5530697.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO179_000517"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO179_000517"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5530698.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO179_000518"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO179_000518"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5530699.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OO179_000519"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OO179_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5530700.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO179_000520"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO179_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5530701.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OO179_000521"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OO179_000521"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5530702.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO179_000522"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO179_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5530703.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OO179_000523"
     CDS             complement(41212..42177)
                     /locus_tag="OO179_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5530704.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO179_000524"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO179_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5530705.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO179_000525"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO179_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5530706.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OO179_000526"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OO179_000526"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5530707.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OO179_000527"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OO179_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5530708.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO179_000528"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO179_000528"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5530709.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO179_000529"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO179_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5530710.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO179_000530"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO179_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5530711.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO179_000531"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO179_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5530712.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO179_000532"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO179_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5530713.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO179_000533"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO179_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5530714.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO179_000534"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO179_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5530715.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OO179_000535"
     CDS             49715..50680
                     /locus_tag="OO179_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5530716.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OO179_000536"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OO179_000536"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5530717.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OO179_000537"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OO179_000537"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5530718.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OO179_000538"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OO179_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5530719.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OO179_000539"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OO179_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5530720.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OO179_000540"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OO179_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5530721.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OO179_000541"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OO179_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5530722.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OO179_000542"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OO179_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5530723.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OO179_000543"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OO179_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5530724.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OO179_000544"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OO179_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5530725.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OO179_000545"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OO179_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5530726.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58505
                     /gene="yrbL"
                     /locus_tag="OO179_000546"
     CDS             57879..58505
                     /gene="yrbL"
                     /locus_tag="OO179_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5530727.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OO179_000547"
                     /pseudo
     CDS             complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OO179_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OO179_000548"
     CDS             complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OO179_000548"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5530728.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OO179_000549"
     CDS             complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OO179_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5530729.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62541..63470)
                     /locus_tag="OO179_000550"
     CDS             complement(62541..63470)
                     /locus_tag="OO179_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5530730.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64145..68605
                     /gene="gltB"
                     /locus_tag="OO179_000551"
     CDS             64145..68605
                     /gene="gltB"
                     /locus_tag="OO179_000551"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5530731.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68618..70036
                     /gene="gltD"
                     /locus_tag="OO179_000552"
     CDS             68618..70036
                     /gene="gltD"
                     /locus_tag="OO179_000552"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5530732.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70220..70699
                     /locus_tag="OO179_000553"
     CDS             70220..70699
                     /locus_tag="OO179_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5530733.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17318 a        19177 c        17549 g        16663 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc aattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccattatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc actttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgataaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatct cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggtggcg ataaagagat ccgccgtgag ttaaagtact
    58021 acgcgcatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg
    58081 ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgccgatttt gacggcaagc
    58141 cttccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac
    58201 tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt
    58261 cgctgaagcc gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg
    58321 tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt
    58381 gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg
    58441 ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag
    58501 cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta
    58561 aaatccacgc ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg
    58621 gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt
    58681 tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga
    58741 tattcaggta aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta
    58801 atccagcttc cagccctttt cgtacccagc tacgcccatc ccataaaaag agattttggc
    58861 cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc
    58921 cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc
    58981 tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga
    59041 atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac
    59101 cgctgagaag ggaacaggcg caacgctaaa caacgcgatg cccccgcccc agaaaacggc
    59161 gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct
    59221 tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct
    59281 tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca
    59341 accacgatat catcgacagg acacggcaca tgctccgcgc ccatctcttc cagcacttct
    59401 gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc
    59461 atcgccgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc
    59521 agcgccttta attcacggtc aacggtgcat tcgctaccaa gactggcaaa attgcttaaa
    59581 ttcttcgccg cgccaacccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc
    59641 tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacattgcgc
    59701 gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt
    59761 gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca
    59821 tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt
    59881 gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca
    59941 tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc
    60001 ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag
    60061 atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt
    60121 ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc
    60181 ttcaatccat tcaccgacgt tatcttccca ggcgggaagg tcaggagact gaatttgctg
    60241 acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc
    60301 ttcctcaaca atgccttttt tatcctgcgc cgtcagattc aactccagca cgctgacata
    60361 gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag
    60421 ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc
    60481 tgtcgtcacc gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt
    60541 tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc
    60601 ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg
    60661 ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc
    60721 cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat
    60781 ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag
    60841 ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa
    60901 cgcatcatcg acctcttctg ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc
    60961 tttgccctgt tcactggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga
    61021 aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg
    61081 gtaatacatg gcgaagattt tatccagctc atcctgcgga atgccgatac cagaatcttc
    61141 cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg
    61201 ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc
    61261 gtcggtaatg acctgatgcg gtaatggcag cgtaggctcc aggttaaagc gcaatccttt
    61321 ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac
    61381 cggctgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt
    61441 aaagatattc cccagcgtga cggccgaaac atggatagtt ttgagatatt tttcctgctc
    61501 ggcggtgagt tcggtatcca gcagaatgcg gctcaggccg acgataccgt tcagcggtgt
    61561 acgcaattcg tgactgatgg tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag
    61621 cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg
    61681 tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc
    61741 cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc
    61801 ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac
    61861 cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc
    61921 tttatcttcg ttacgataaa aaaccaagtc gggtgaagca tcaaggaagg aacgtaagaa
    61981 tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag
    62041 ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcaa tttcctgatt
    62101 tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc
    62161 ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac
    62221 caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat
    62281 aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat
    62341 ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat
    62401 catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca
    62461 cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat
    62521 gcggaattcc cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat
    62581 ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt
    62641 tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag
    62701 atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtata atcctccagt
    62761 tcaataccgt tcaaacgcac cgcttcccag gctttcgcca taatgctgcc tttcacaata
    62821 tgcagcggat gcagctttat gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc
    62881 aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca caccttcagc
    62941 ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg gccgcggttg
    63001 atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca cacttcgtaa
    63061 ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg
    63121 gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata catagaacgc
    63181 agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta
    63241 gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc
    63301 gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc gatggtaccg
    63361 tcacggttag ggcagctaaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa
    63421 cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag
    63481 accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg
    63541 gtcaatcgtc ccggcctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca
    63601 tgcacaatca cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg
    63661 attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta taaaatgctg
    63721 gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt
    63781 gactgtttct ctaacgactc tccttttagc cttaaagata aaatctattt taatttcagc
    63841 tatttaataa agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac
    63901 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
    63961 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
    64021 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
    64081 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
    64141 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
    64201 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
    64261 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
    64321 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
    64381 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
    64441 ccgcatcgtt gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
    64501 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac gcattgagca
    64561 aatttttgtg aacgccccgg caggctggcg tccacgcgat atggagcgcc gtctgtttat
    64621 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
    64681 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
    64741 tctggatctt gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc
    64801 cactaacacc gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg
    64861 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
    64921 gacgccgctg atccctgacc tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga
    64981 ctccagttcc atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
    65041 cgccatgcgc ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact
    65101 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc cggcgggcat
    65161 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
    65221 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
    65281 ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
    65341 cgacactcgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
    65401 ccacccttat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
    65461 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
    65521 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
    65581 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
    65641 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
    65701 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
    65761 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
    65821 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
    65881 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa
    65941 agccgaaaaa atggtacgta gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc
    66001 taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga cccgtctggt
    66061 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
    66121 gcaccacttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt accttgccta
    66181 tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc gtaccgtgat
    66241 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
    66301 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga
    66361 tgtagtgggc ctgtgcttcc agggcgcggt cagccgcatt ggtggggcaa gctttgaaga
    66421 cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca agccgattag
    66481 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt
    66541 ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc
    66601 gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg
    66661 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
    66721 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
    66781 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
    66841 tggcactaac aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc
    66901 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
    66961 cgaaggtggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
    67021 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgaaga
    67081 cttagcgcag cttattttcg acctcaagca ggttaacccg aaagcgatga tctccgttaa
    67141 gctggtgtcc gaaccaggcg ttggcaccat cgcgactggc gtggcgaaag cttatgcgga
    67201 cttgatcacc atcgcgggct atgacggcgg caccggcgca agcccgctgt cgtcagtgaa
    67261 atatgcaggc tgcccgtggg aactcggtct ggtggaaacc caacaggcgc tggttgccaa
    67321 cggcttgcgt cataaaattc gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat
    67381 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtggc
    67441 gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac
    67501 tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgactaatta
    67561 ctttgagttt atcgcccgtg aaacccgcga gctgatggca cagctgggag taacgcgtct
    67621 ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca
    67681 gcagaaactg gcgctgtcga agctgctgga gactgccgag ccacatgcag gtaagtcact
    67741 ctactgcact gaaaacaacc cgccgtttga taacggccta ctgaacgcgc agttgctgca
    67801 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa
    67861 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
    67921 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
    67981 tgtgtggaac gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg
    68041 taaaggcatg gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag
    68101 ccatgaagca agcattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
    68161 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
    68221 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
    68281 taaaaccggc gttaacttcg gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga
    68341 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga
    68401 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcataccgg
    68461 ttctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct
    68521 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc gtagcgcagc
    68581 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
    68641 tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgta aaattgagtt
    68701 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgtct
    68761 gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca tcccaaactg
    68821 gctgaagctc gccaacgagg ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa
    68881 cacactgccg gaagtatgcg gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg
    68941 caccctgaac gatgagttcg gtgcggtgac catcggcaac attgagcgtt atatcaatga
    69001 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
    69061 agtggcgatt atcggtgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
    69121 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
    69181 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcacagg
    69241 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc tggacgatct
    69301 gctgagtgat tacgatgccg tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg
    69361 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
    69421 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
    69481 acgcgtggta gtacttggcg gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg
    69541 ccagggggca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
    69601 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
    69661 actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga
    69721 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
    69781 tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca acatggaatg
    69841 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgctc cggaaggcaa
    69901 cgacaacgct ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
    69961 ttccgatctg gtcgttaccg ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa
    70021 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
    70081 attctccttt ctttctggaa atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
    70141 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
    70201 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
    70261 aatccacaac ggcattattc atctggtcga tagcgcccga acggaaacgg tacgtagcgt
    70321 taacgcgctg atgaccgcga cgtactggga aattggccga cggattgtcg aatttgaaca
    70381 aggtggtgag gccagagcag cttatggtgc acagttgatt gatcggctat cgaaggattt
    70441 aagtcgacgg tataaacgtg gggtctctgc aaaaaactta cgccaaatga ggctttttta
    70501 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
    70561 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
    70621 caaattgaga ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt
    70681 tgtatattca tgcaaataaa cgaagga
//
LOCUS       DAJTNW010000008        67100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNW010000008 DAJTNW010000000
VERSION     DAJTNW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO179_000554"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO179_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5530734.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OO179_000555"
     CDS             complement(1150..1236)
                     /locus_tag="OO179_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5530735.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO179_000556"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO179_000556"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5530736.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO179_000557"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO179_000557"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5530737.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="OO179_000558"
     CDS             3585..3629
                     /locus_tag="OO179_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530738.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO179_000559"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO179_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5530739.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO179_000560"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO179_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5530740.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO179_000561"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO179_000561"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5530741.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO179_000562"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO179_000562"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5530742.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO179_000563"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO179_000563"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5530743.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO179_000564"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO179_000564"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5530744.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO179_000565"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO179_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5530745.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO179_000566"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO179_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5530746.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO179_000567"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO179_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5530747.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO179_000568"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO179_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5530748.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO179_000569"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO179_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5530749.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO179_000570"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO179_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5530750.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO179_000571"
     CDS             complement(16981..17745)
                     /locus_tag="OO179_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5530751.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO179_000572"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO179_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5530752.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO179_000573"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO179_000573"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5530753.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO179_000574"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO179_000574"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5530754.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO179_000575"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO179_000575"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5530755.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO179_000576"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO179_000576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5530756.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO179_000577"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO179_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5530757.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO179_000578"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO179_000578"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5530758.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO179_000579"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO179_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5530759.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO179_000580"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO179_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5530760.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO179_000581"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO179_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5530761.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO179_000582"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO179_000582"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5530762.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO179_000583"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO179_000583"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5530763.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO179_000584"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO179_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5530764.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO179_000585"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO179_000585"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5530765.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO179_000586"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO179_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5530766.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO179_000587"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO179_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5530767.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO179_000588"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO179_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5530768.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO179_000589"
     CDS             complement(40421..41815)
                     /locus_tag="OO179_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5530769.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO179_000590"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO179_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5530770.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO179_000591"
     CDS             complement(42158..43444)
                     /locus_tag="OO179_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5530771.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO179_000592"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO179_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO179_000593"
     CDS             complement(44451..45221)
                     /locus_tag="OO179_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5530772.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="OO179_000594"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="OO179_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5530773.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKAGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="OO179_000595"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="OO179_000595"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5530774.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="OO179_000596"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="OO179_000596"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5530775.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="OO179_000597"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="OO179_000597"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5530776.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="OO179_000598"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="OO179_000598"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5530777.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="OO179_000599"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="OO179_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5530778.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OO179_000600"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OO179_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5530779.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OO179_000601"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OO179_000601"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5530780.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OO179_000602"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OO179_000602"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5530781.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OO179_000603"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OO179_000603"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5530782.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OO179_000604"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OO179_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5530783.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OO179_000605"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OO179_000605"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5530784.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OO179_000606"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OO179_000606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5530785.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OO179_000607"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OO179_000607"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5530786.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OO179_000608"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OO179_000608"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5530787.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OO179_000609"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OO179_000609"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5530788.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="OO179_000610"
     CDS             complement(66187..66405)
                     /locus_tag="OO179_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5530789.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="OO179_000611"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="OO179_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5530790.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16292 a        18260 c        17265 g        15283 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 aacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 ccggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccaag caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DAJTNW010000009        65688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNW010000009 DAJTNW010000000
VERSION     DAJTNW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO179_000612"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO179_000612"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5530791.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO179_000613"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO179_000613"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5530792.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO179_000614"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO179_000614"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5530793.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO179_000615"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO179_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5530794.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO179_000616"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO179_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5530795.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO179_000617"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO179_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5530796.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO179_000618"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO179_000618"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5530797.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO179_000619"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO179_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5530798.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO179_000620"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO179_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5530799.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO179_000621"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO179_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5530800.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO179_000622"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO179_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5530801.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO179_000623"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO179_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5530802.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO179_000624"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO179_000624"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5530803.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO179_000625"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO179_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5530804.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO179_000626"
     CDS             19304..19369
                     /locus_tag="OO179_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530805.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO179_000627"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO179_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5530806.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO179_000628"
     CDS             complement(19753..20376)
                     /locus_tag="OO179_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5530807.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO179_000629"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO179_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5530808.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO179_000630"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO179_000630"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5530809.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO179_000631"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO179_000631"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5530810.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO179_000632"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO179_000632"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5530811.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO179_000633"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO179_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO179_000634"
     CDS             complement(26460..26846)
                     /locus_tag="OO179_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5530812.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO179_000635"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO179_000635"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5530813.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO179_000636"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO179_000636"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5530814.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO179_000637"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO179_000637"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5530815.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO179_000638"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO179_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5530816.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO179_000639"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO179_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5530817.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO179_000640"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO179_000640"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5530818.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO179_000641"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO179_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5530819.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO179_000642"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO179_000642"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5530820.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO179_000643"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO179_000643"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5530821.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO179_000644"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO179_000644"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5530822.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO179_000645"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO179_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5530823.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO179_000646"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO179_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5530824.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO179_000647"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO179_000647"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5530825.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO179_000648"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO179_000648"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5530826.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO179_000649"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO179_000649"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5530827.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO179_000650"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO179_000650"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5530828.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO179_000651"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO179_000651"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5530829.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO179_000652"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO179_000652"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5530830.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO179_000653"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO179_000653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5530831.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO179_000654"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO179_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5530832.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO179_000655"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO179_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5530833.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO179_000656"
     CDS             53914..54303
                     /locus_tag="OO179_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5530834.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO179_000657"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO179_000657"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5530835.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO179_000658"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO179_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5530836.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLILTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO179_000659"
     CDS             complement(55962..56162)
                     /locus_tag="OO179_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5530837.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO179_000660"
     CDS             56328..56873
                     /locus_tag="OO179_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5530838.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO179_000661"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO179_000661"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5530839.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO179_000662"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO179_000662"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5530840.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO179_000663"
     CDS             complement(58171..58995)
                     /locus_tag="OO179_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5530841.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO179_000664"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO179_000664"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5530842.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO179_000665"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO179_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5530843.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO179_000666"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO179_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5530844.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO179_000667"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO179_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5530845.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO179_000668"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO179_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5530846.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO179_000669"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO179_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5530847.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO179_000670"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO179_000670"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5530848.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15529 a        16278 c        18103 g        15778 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct ggggggtcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcagcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgtggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgatagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagattcaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg ggagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gtgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctatgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacatctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctacca
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagtattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacga
//
LOCUS       DAJTNW010000010        65614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNW010000010 DAJTNW010000000
VERSION     DAJTNW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO179_000671"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO179_000671"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5530849.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO179_000672"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO179_000672"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5530850.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO179_000673"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO179_000673"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5530851.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO179_000674"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO179_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5530852.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO179_000675"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO179_000675"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5530853.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO179_000676"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO179_000676"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5530854.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO179_000677"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO179_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5530855.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO179_000678"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO179_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5530856.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO179_000679"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO179_000679"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5530857.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO179_000680"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO179_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5530858.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO179_000681"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO179_000681"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5530859.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO179_000682"
     CDS             complement(15666..16703)
                     /locus_tag="OO179_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5530860.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OO179_000683"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OO179_000683"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5530861.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO179_000684"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO179_000684"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5530862.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OO179_000685"
     CDS             19445..19885
                     /locus_tag="OO179_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5530863.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO179_000686"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO179_000686"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5530864.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OO179_000687"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OO179_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO179_000688"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO179_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5530865.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO179_000689"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO179_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5530866.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO179_000690"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO179_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5530867.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO179_000691"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO179_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5530868.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO179_000692"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO179_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5530869.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO179_000693"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO179_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5530870.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO179_000694"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO179_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5530871.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO179_000695"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO179_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5530872.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OO179_000696"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OO179_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5530873.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO179_000697"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO179_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5530874.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO179_000698"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO179_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5530875.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO179_000699"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO179_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5530876.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO179_000700"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO179_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5530877.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO179_000701"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO179_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5530878.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OO179_000702"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OO179_000702"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5530879.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OO179_000703"
     CDS             37413..37682
                     /locus_tag="OO179_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5530880.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="OO179_000704"
     CDS             complement(37685..38044)
                     /locus_tag="OO179_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5530881.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="OO179_000705"
     CDS             38185..38811
                     /locus_tag="OO179_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5530882.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="OO179_000706"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="OO179_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5530883.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO179_000707"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO179_000707"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5530884.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="OO179_000708"
     CDS             41651..42316
                     /locus_tag="OO179_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5530885.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="OO179_000709"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="OO179_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5530886.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="OO179_000710"
     CDS             complement(42950..44167)
                     /locus_tag="OO179_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5530887.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="OO179_000711"
     CDS             44301..45350
                     /locus_tag="OO179_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5530888.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="OO179_000712"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="OO179_000712"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5530889.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="OO179_000713"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="OO179_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5530890.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="OO179_000714"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="OO179_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5530891.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="OO179_000715"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="OO179_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5530892.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="OO179_000716"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="OO179_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5530893.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="OO179_000717"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="OO179_000717"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5530894.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="OO179_000718"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="OO179_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5530895.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="OO179_000719"
     CDS             complement(51545..51904)
                     /locus_tag="OO179_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5530896.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="OO179_000720"
     CDS             complement(51901..52176)
                     /locus_tag="OO179_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5530897.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="OO179_000721"
     CDS             complement(52235..53359)
                     /locus_tag="OO179_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5530898.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO179_000722"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO179_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5530899.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="OO179_000723"
     CDS             complement(56091..57158)
                     /locus_tag="OO179_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5530900.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEKLPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="OO179_000724"
     CDS             complement(57524..59146)
                     /locus_tag="OO179_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5530901.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="OO179_000725"
     CDS             complement(59408..60997)
                     /locus_tag="OO179_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5530902.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61382..62413
                     /locus_tag="OO179_000726"
                     /pseudo
     CDS             61382..62413
                     /locus_tag="OO179_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62500..62976)
                     /locus_tag="OO179_000727"
     CDS             complement(62500..62976)
                     /locus_tag="OO179_000727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530903.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62969..63733)
                     /locus_tag="OO179_000728"
     CDS             complement(62969..63733)
                     /locus_tag="OO179_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5530904.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63979..64740
                     /locus_tag="OO179_000729"
     CDS             63979..64740
                     /locus_tag="OO179_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5530905.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64847..65347)
                     /locus_tag="OO179_000730"
     CDS             complement(64847..65347)
                     /locus_tag="OO179_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530906.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15938 a        17620 c        16161 g        15895 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgca gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgacaca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaacg gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcaacac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag catgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg ggagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggctagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaagatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcga gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gctcttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcaggttaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaatagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctggcgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtgc attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgc caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actgaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cgcgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc gcgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt tcttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaagccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcact tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggccgga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agtttttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcttgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ctttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaagat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaaa caacagaata agaattaatc
    61201 ttaggataaa tatttattta tcatggcctt ttgaccgcta aaaaatggca ctcggcacag
    61261 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
    61321 caggctgaaa ttatcattaa tgcacatttc aatattagca gggatacctg aggagtttaa
    61381 agtgaacata tatatcgggt agcttttcaa attaatccct ttgattatag gcttaatttg
    61441 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa cgtcacgggt catgtgctga
    61501 tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc atttcgcaac
    61561 tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc tatgcggggt
    61621 caattatcac catgatatgg ggttgggcac tgttagcagg taatgatgtg atggcagacg
    61681 agtttgttgc cggccatgtt atttttggcg ttggtatgat tgccgcatgt gtatcgacgg
    61741 tggcagcgtc gtccggtcac tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg
    61801 acggaacacc ggtacaggct tattcttcat taatcggtaa ttgcctcatt gccgttcccg
    61861 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacatagt gccgacataa
    61921 ctccgcatta tgtcgccttg ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc
    61981 cacaatcgtc catcaaacac gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta
    62041 ttgggttatt tttctcggct cagtcacggt gctgcagggg atatacgtct tagtcagttc
    62101 cgatgcaagc gcccgactgg ctcccggcat tattctcatt tgcctcggaa tgatctgtta
    62161 cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct gttcgttagc
    62221 caacagaata ccgatgatac cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt
    62281 cctcgcggaa atcgcacaaa ctgagctgga ctactttatt ccatctcggg ttctggttgg
    62341 attaggcgca gtatgtttta cattgttctc tattgtttca atattagagg ccggttctgc
    62401 gaaaaaaaat taaaatttac ataatcccag cgttattcat atcttaacgc tggtttttta
    62461 cgaacttagt gaaataatgt agaatataaa attataattt tatattggag cacatgtgtt
    62521 aatgatcaac cccgggatac taaaaatata gacattatta aacttgctta gcctcgcatt
    62581 gcctttacct gttaaagatg catcgagaaa attgagtctg aagttctcat cctttatctg
    62641 attttccagt cccgaaactt tggccttagc attacctgag atccgtccgt tactatctat
    62701 tttgtaggta aaaaaatacg aatgggaaaa tttcctcggt gtatcttcat cagttaagcc
    62761 aacaatcgta aaactaccgc taccatcttt ttttaaaatg actgaaaggt tagcagagta
    62821 gtataaagaa tgatctgttt tagcatttct tgttaacgtc gacgtacatt caagagacgt
    62881 tgtgctacca tcacgagcct tccagagagc aaaaaaacac acctgtagca atgtgaaaac
    62941 aagtattaat ataaaataca ttttcttatt agtcatataa tagcacctct gaacaaattg
    63001 cataagtttc gtcattagag gattttatac atcttgataa ataccgctgc ccttccccaa
    63061 gcaaataatg catactattt acatcatata taaaatttgc acctggcaca cagggtaaat
    63121 gctttttcga aatttcagcg gcctctattt gagatttagc gaggccaatc tcgttaagat
    63181 tctctgaaag atataaatca caggtgtcaa tcttacctga tttaaccaat ggataatccg
    63241 ttatactgta ggtatcttta aaagccacgg caaatatttc aataataaca attactacca
    63301 caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact ttttcaacaa
    63361 catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg ttttcactga
    63421 tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca atatttttaa
    63481 aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa
    63541 atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca ttactttcca
    63601 gtaacactgc caaaatacac cccgccgtag ctggcaaatt tatctcatcc tgggtattta
    63661 cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata atgaaaaaac
    63721 atctccgctc catgcaggca acaacacaaa ataagatttt gtcattttag cctattcctg
    63781 gtggaactga cacaagaaac taaaatacaa atataaccct gaaaaagtca tataaaactt
    63841 atatccacag aataaaaaat caaacatgtc agttttatca atataaagta tcgcaaggcg
    63901 aaaagtgatg caagaatact cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac
    63961 atgtaatttc atcattaggt gctatcgaaa ataactcagg agctacttaa gatgaataat
    64021 gtcttactca tgagacccga tgactcagaa tttatgatac ctgatgactg gtcagggcta
    64081 aacgcaaaag gcattacata cgaacaagat aatagagtga cagagtcttt tctgcctttt
    64141 aaatcttttt attgttcacc ggtgtgcacg acagttgaac tcatctcaca aaataattgt
    64201 tttgctatgg gtatccgcta ccttctggaa aagaaaagag acttaaggct aaatcacttt
    64261 aaagccttct ctcataaatg cgagacaaaa cctgacgtgt taattatgac cgggtgcacc
    64321 gtcgctgata cttatagtat attctcacgc attgttgaag aaaatataat gaaaacgatg
    64381 acacctatcg tcatcattac tgggcatcac tcagaagatc tttgttctct ggcatcgaaa
    64441 ctgggaggag ttaaaataat atcaccgaca gaaaagattg caaaaataga aaacgctatc
    64501 aatgattctt ttaacagcaa gcctaaaaaa ataagatttt taacagatat actctctcct
    64561 caacaaattg agaccatgaa aatggttgcg cagggtagat ccgttaatga gatttcagcc
    64621 gcaatgaata ttagcatcaa gaccgtgtat gcacataaag ttcgtgctat caataagctg
    64681 ggaattaaga aaaaagttta tgaagcgctt ttttataaag cgctaattca gttcaactga
    64741 tgtaaaaatc accctcgctt aataaataat atattctatg tcttttttta taagttaaaa
    64801 aaggcgggat atcaaacccc gccatgaaat taactaatca tctgcattat tctgcgtaga
    64861 tataagcctg ggtacggatg gtgtaagagt ctggctcaac aacctgttct ttcatatggg
    64921 aaacgacgcg ataggcacca cctgttccag tatttttcag catcatataa cgcttaccgc
    64981 caatctcttc ggaggcagct gaaggaggat cctgctcaag cgttgtcagg gaaactttca
    65041 gtttatggtc cgggttattg tcaccagaaa ttactgcatc cgctgaagta ctgctctccg
    65101 cataaatatc atccgggaaa gttacagcat agtcgtggct ctgcccatca gatgcgccaa
    65161 caatacctgt tgccaaaggc gtccatgcag ttaacgtacc gctatatacc cctttcactg
    65221 gttgaatggt attattaaca acgataggct tggtcaaagt aagattagct gacgcttcag
    65281 taccctggct tactaaagtt cccgcctgag aagcaaaagc tgtccccgcc agcatgattg
    65341 ctaacaatgt tttcttcatt atattttctc catgattttc atttacactg caaaatgcag
    65401 tttctttctt tgcatttacg tgctatcgcg aaaatgcttc caccaaataa tggaaacatc
    65461 ttcacaaaaa taaattatga gtttcttaat ttagaaacta taaacatgtc gccgctatct
    65521 ggtataaccc tgatgcactt ttcttcgcag gaagttggta ttttgcgata caggattgat
    65581 ctttagaata accccatttc acattaaact cgcc
//
LOCUS       DAJTNW010000011        65473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNW010000011 DAJTNW010000000
VERSION     DAJTNW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            133..576
                     /locus_tag="OO179_000731"
     CDS             133..576
                     /locus_tag="OO179_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5530907.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="OO179_000732"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="OO179_000732"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5530908.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO179_000733"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO179_000733"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5530909.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO179_000734"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO179_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5530910.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO179_000735"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO179_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5530911.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO179_000736"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO179_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5530912.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO179_000737"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO179_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5530913.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO179_000738"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO179_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5530914.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO179_000739"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO179_000739"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5530915.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO179_000740"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO179_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5530916.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO179_000741"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO179_000741"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5530917.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO179_000742"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO179_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5530918.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO179_000743"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO179_000743"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5530919.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO179_000744"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO179_000744"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5530920.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="OO179_000745"
     CDS             13936..14316
                     /locus_tag="OO179_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5530921.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO179_000746"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO179_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5530922.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO179_000747"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO179_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5530923.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO179_000748"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO179_000748"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5530924.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO179_000749"
     tRNA            complement(17417..17492)
                     /locus_tag="OO179_000749"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO179_000750"
     tRNA            complement(17496..17571)
                     /locus_tag="OO179_000750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO179_000751"
     tRNA            complement(17623..17698)
                     /locus_tag="OO179_000751"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO179_000752"
     tRNA            complement(17701..17776)
                     /locus_tag="OO179_000752"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OO179_000753"
     tRNA            complement(17913..17988)
                     /locus_tag="OO179_000753"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO179_000754"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO179_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5530925.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO179_000755"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO179_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5530926.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO179_000756"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO179_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5530927.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22176)
                     /gene="tolA"
                     /locus_tag="OO179_000757"
     CDS             complement(20935..22176)
                     /gene="tolA"
                     /locus_tag="OO179_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5530928.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22241..22669)
                     /gene="tolR"
                     /locus_tag="OO179_000758"
     CDS             complement(22241..22669)
                     /gene="tolR"
                     /locus_tag="OO179_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5530929.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22673..23365)
                     /gene="tolQ"
                     /locus_tag="OO179_000759"
     CDS             complement(22673..23365)
                     /gene="tolQ"
                     /locus_tag="OO179_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5530930.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23362..23766)
                     /gene="ybgC"
                     /locus_tag="OO179_000760"
     CDS             complement(23362..23766)
                     /gene="ybgC"
                     /locus_tag="OO179_000760"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5530931.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23916..24209)
                     /gene="ybgE"
                     /locus_tag="OO179_000761"
     CDS             complement(23916..24209)
                     /gene="ybgE"
                     /locus_tag="OO179_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5530932.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24209..24322)
                     /gene="cydX"
                     /locus_tag="OO179_000762"
     CDS             complement(24209..24322)
                     /gene="cydX"
                     /locus_tag="OO179_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5530933.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24337..25476)
                     /gene="cydB"
                     /locus_tag="OO179_000763"
     CDS             complement(24337..25476)
                     /gene="cydB"
                     /locus_tag="OO179_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5530934.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25492..27060)
                     /gene="cydA"
                     /locus_tag="OO179_000764"
     CDS             complement(25492..27060)
                     /gene="cydA"
                     /locus_tag="OO179_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5530935.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27909..30542)
                     /gene="mngB"
                     /locus_tag="OO179_000765"
                     /pseudo
     CDS             complement(27909..30542)
                     /gene="mngB"
                     /locus_tag="OO179_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="OO179_000766"
     CDS             complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="OO179_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5530936.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32646..33368
                     /gene="mngR"
                     /locus_tag="OO179_000767"
     CDS             32646..33368
                     /gene="mngR"
                     /locus_tag="OO179_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5530937.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="OO179_000768"
     CDS             complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="OO179_000768"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5530938.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="OO179_000769"
     CDS             complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="OO179_000769"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5530939.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="OO179_000770"
     CDS             complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="OO179_000770"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5530940.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="OO179_000771"
     CDS             complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="OO179_000771"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5530941.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="OO179_000772"
     CDS             complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="OO179_000772"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5530942.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="OO179_000773"
     CDS             complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="OO179_000773"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5530943.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="OO179_000774"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="OO179_000774"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5530944.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="OO179_000775"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="OO179_000775"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5530945.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43558..43665
                     /locus_tag="OO179_000776"
     CDS             43558..43665
                     /locus_tag="OO179_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530946.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43871..45154
                     /gene="gltA"
                     /locus_tag="OO179_000777"
     CDS             43871..45154
                     /gene="gltA"
                     /locus_tag="OO179_000777"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5530947.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45545..46111
                     /locus_tag="OO179_000778"
     CDS             45545..46111
                     /locus_tag="OO179_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5530948.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46162..48621
                     /locus_tag="OO179_000779"
     CDS             46162..48621
                     /locus_tag="OO179_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5530949.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48636..49367
                     /locus_tag="OO179_000780"
     CDS             48636..49367
                     /locus_tag="OO179_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5530950.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49364..50440
                     /locus_tag="OO179_000781"
                     /pseudo
     CDS             49364..50440
                     /locus_tag="OO179_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50592..51638
                     /locus_tag="OO179_000782"
     CDS             50592..51638
                     /locus_tag="OO179_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5530951.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51635..52426)
                     /gene="nei"
                     /locus_tag="OO179_000783"
     CDS             complement(51635..52426)
                     /gene="nei"
                     /locus_tag="OO179_000783"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5530952.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52462..53196)
                     /gene="pxpA"
                     /locus_tag="OO179_000784"
     CDS             complement(52462..53196)
                     /gene="pxpA"
                     /locus_tag="OO179_000784"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5530953.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53186..54118)
                     /gene="pxpC"
                     /locus_tag="OO179_000785"
                     /pseudo
     CDS             complement(53186..54118)
                     /gene="pxpC"
                     /locus_tag="OO179_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54112..54768)
                     /gene="pxpB"
                     /locus_tag="OO179_000786"
     CDS             complement(54112..54768)
                     /gene="pxpB"
                     /locus_tag="OO179_000786"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5530954.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54791..55534)
                     /gene="ybgI"
                     /locus_tag="OO179_000787"
     CDS             complement(54791..55534)
                     /gene="ybgI"
                     /locus_tag="OO179_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5530955.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55805..57286
                     /gene="dtpD"
                     /locus_tag="OO179_000788"
     CDS             55805..57286
                     /gene="dtpD"
                     /locus_tag="OO179_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5530956.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57436..58854)
                     /gene="phrB"
                     /locus_tag="OO179_000789"
     CDS             complement(57436..58854)
                     /gene="phrB"
                     /locus_tag="OO179_000789"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5530957.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58851..59360)
                     /locus_tag="OO179_000790"
     CDS             complement(58851..59360)
                     /locus_tag="OO179_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5530958.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59489..59776
                     /locus_tag="OO179_000791"
     CDS             59489..59776
                     /locus_tag="OO179_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5530959.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59831..60211)
                     /locus_tag="OO179_000792"
     CDS             complement(59831..60211)
                     /locus_tag="OO179_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530960.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60214..64878)
                     /locus_tag="OO179_000793"
                     /pseudo
     CDS             complement(60214..64878)
                     /locus_tag="OO179_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(64897..65100)
                     /locus_tag="OO179_000794"
                     /pseudo
     CDS             complement(64897..65100)
                     /locus_tag="OO179_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15869 a        17735 c        15983 g        15886 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccacaa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatct cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcta tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag caaacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacgtttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct cagcttctgc
    21541 cttggctttt tcagcagctt cagttgctgc tttctttctt gcttcagctg cagctgcttc
    21601 tgccgcttcc gctttcttct tcagtgccgc agcggctacc tcggcttttt tctgcgcttc
    21661 ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt
    21721 cgctgcttct tccgcagctt tagcatctgc cgccgctttc gctgccgcca cttcagcttg
    21781 cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct gctcctgagc
    21841 cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg
    21901 cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac gcttcgcgct
    21961 tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac
    22021 agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct catcgaacga
    22081 actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg
    22141 cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc
    22201 ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta
    22261 aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt
    22321 aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc
    22381 tggacacttc cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca
    22441 ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc
    22501 tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg
    22561 gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac ggaacaatgt
    22621 tgatttcgga cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct
    22681 tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa
    22741 agttgtcgta attcagttcc agtttgttta cgcgctggtt gagacggtta taagccatta
    22801 ccgccggaat tgcggcgaac agaccaattg ccgtcgcaat caacgcttct gcgataccgg
    22861 gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag gcgtgcatga
    22921 tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc gtaccgagga
    22981 acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc atagcacgcg
    23041 acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaact
    23101 ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt ttcccctgac
    23161 tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg
    23221 cttcacgcgc cgctgcgtta agaatacggg ttcgctggat aataatggcc caggatgcga
    23281 ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca
    23341 aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc
    23401 acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa
    23461 agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt
    23521 tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt
    23581 cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac tgaagtgatg
    23641 atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac
    23701 cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt
    23761 attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac
    23821 gcgcgcgatt gaggtttggg aatgagggca agttaagggc gcgtaaaaat ttatgggcct
    23881 ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg
    23941 acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag aaccttctgc
    24001 ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag caataaacca
    24061 tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca aaacatacat
    24121 cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt gtccattacc
    24181 gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt
    24241 cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca
    24301 gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg
    24361 ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag
    24421 cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct
    24481 ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa
    24541 cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac
    24601 aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagca gcagaaccac
    24661 acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc
    24721 agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt ccattgtcga
    24781 tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg cgaaacagac
    24841 cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca gttcgcccac
    24901 ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc tcaccacgcc
    24961 tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt acagacgcag
    25021 atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta caccaattac
    25081 cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac gccagcgggt
    25141 ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag acgccagcac
    25201 gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg gccaggcagc
    25261 aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt gtggtgcaat
    25321 ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc
    25381 cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc caaccagcag
    25441 ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt cttagcgtgc
    25501 cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca ggctgcttgg
    25561 gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca gggtatacag
    25621 gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg aattcgccac
    25681 agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag ccacgaacca
    25741 gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc gcagaagcca
    25801 ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga tgattgccag
    25861 cagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg
    25921 gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt
    25981 atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga actggtcacg
    26041 aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg ccttcatccc
    26101 gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa ccggggtatc
    26161 cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt
    26221 ctcctcttcc tgatcaggaa tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc
    26281 ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt cgtagccgga
    26341 ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg cagcgatagc
    26401 aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg cgctgatacc
    26461 gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga atttcacctg
    26521 agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca tagtttcaaa
    26581 gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa tccacagtgc
    26641 ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa ctttacccag
    26701 acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga aggccatcag
    26761 accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat agtaagacca
    26821 gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag cgaagttgat
    26881 accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc cggagaggac
    26941 gtagaccgtt tccataatgg ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag
    27001 gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta tatctaacat
    27061 catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga aagggtcaca
    27121 cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa
    27181 aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc ccacgtatat
    27241 attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt gcatttttcg
    27301 acggtgatca atttatagct aaattgccgc ctttcagcca atttgatcga gaacaattta
    27361 tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt tctacaaatt
    27421 atgttaatca tgtttcaaca aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt
    27481 ataagactga aattagtttc ggttggcaat taaagataaa aagagacgaa gcaaaaacag
    27541 cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt
    27601 attaataaag gcataaatct cccccccgcc gatattaaca tttaattaac ttataattat
    27661 attataatta actcacccgt ggttattatt ttgttaaaaa taaagccaca tatacgtagc
    27721 ttgagagtga agataaacac aaagttacct gatgcgctac gcttgccagt gtctacatca
    27781 tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt catgccgtat
    27841 ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg cttcggtttg
    27901 attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc
    27961 gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat cgtttctgag
    28021 caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga ttcagccgga
    28081 ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg
    28141 catcccactg ggggcatttt caacaaacta taactttccg gcacgttgaa tccggctttg
    28201 ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt cagccatgct
    28261 cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa taaacttagg
    28321 cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat tcccgaaggc
    28381 cgcctaggcc ttaaaagcag atcttctttc cccagtaagc ccacgccgcg cagcaacgta
    28441 atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag
    28501 acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt ccataccgga
    28561 accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc gttcacgggg
    28621 cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa agatgtaggg
    28681 atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc cacatcaatg
    28741 cgcctgttgt tatgactaag agtgacaacc aactcgacat ctactctgcc agtggattgt
    28801 cttgcgctgc gttgttgcag attacgcggc accgccattt catagcggat aacagccctg
    28861 ctctgccagg cctcatgaat aatctcgcat tgcggtttcg cattcgccga agtaattacc
    28921 cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct ttcttcaatt
    28981 tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag cgttccatcc
    29041 tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc actttttgcc
    29101 gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc catagaaggg
    29161 acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc gtaatgaact
    29221 atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa atacggtaca
    29281 ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac agtggtgttg
    29341 atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc
    29401 tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc cgccatgtct
    29461 tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcactaca gcagcagccg
    29521 atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag caagccgtgg
    29581 tggtatccaa aacccaacgt cctggccagc gttgccagcg gttccagcag attaacaatc
    29641 ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaacc gatggtgcga
    29701 tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag attatctcgc
    29761 tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt tcgttgagga
    29821 taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg catctgatca
    29881 tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac gtcaaaataa
    29941 ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc gatggcataa
    30001 cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg
    30061 gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca ggtaatgcca
    30121 aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg taaataacct
    30181 attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag attacggacg
    30241 atggattccg cagaaacaat cgtggtatcg gtctgggtat actaggggcc gataatcaac
    30301 ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccggtttcac cgcgaaataa
    30361 tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc
    30421 agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc ttcggtggtg
    30481 aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc
    30541 atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg ccatgcttaa
    30601 ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc accaatgccg
    30661 caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga agtaaaaaga
    30721 gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc
    30781 ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct tcaatcgcca
    30841 tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat ttcccggtct
    30901 caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg
    30961 ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg
    31021 cgcccaggca gaatgcataa gcggctttat tcactggccc tccaaggtca aaggaacaca
    31081 taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg ttcagccagg
    31141 cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc atcagactgc
    31201 ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca ttgaatttac
    31261 tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc aatccgccaa
    31321 cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc gcaaagcctg
    31381 gcgctaacgc cggtttatct gccagcgaat aggcggtata ggctgccagc accggtacca
    31441 tcaaaattcc gagcagcccg ccgcccagtt tgcggtacat ccacagccag gagttttctt
    31501 cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc gccagcaccg
    31561 tcccccccgc gacaatcaac ggtacggcaa aagagatccc gctcaacagt gcctgtttca
    31621 gctccgtttt gacgcttttc accggttgcg tatcttgttg taaggtacgc gtctcatcgc
    31681 cacgctcgag ggtaagcgat tgttggatca acgcttctgc atggcgaatc ggctcggcaa
    31741 caggcacaga aagcgcagga atgccgttaa aacgctcact ctccttgatg gcgacttcag
    31801 ccgcaaaaat acaggcagtt gcactattga gttgatccgc cgttaaacgc ccttcaatac
    31861 cgttagcacc ttgcttttca acgtagacat tcacgccgag tttgcgtccg gctttttcca
    31921 gatactccgc agccatgtag gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga
    31981 tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg
    32041 agagcaattc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca tcatccgcca
    32101 ggcgcgtggt cagcgctgtc agcagttgca tgtgcgtagt acccgcttca ttgaggggaa
    32161 tcgccagcaa caccactaaa tcaaccgctt ccgggccatc cacgccttcc cactgaagcg
    32221 gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg
    32281 gcacagccaa cccttcactt aaggcggtcg ggccaaggct ttcacggcga tacacttctt
    32341 tcagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga gtcaacgcgt
    32401 gaatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg cggtgggtca
    32461 gagtcgtcag gttcatcatg atcctcactt gatcgttttt atagtcgcgc cagtgtgccc
    32521 gacaaaacaa taccattgcg taatcaattt cacttgattg aaattatttg cattttattt
    32581 gtattgttta caggatgaat ttatcgggca taatggctaa attcgtatta atgagatgct
    32641 aaataatggg acacaaaccc ttataccgac agattgctga tcgcattcgt gagcaaattg
    32701 cgcgtggcga gcttaaacct ggcgatgcgt tacctaccga atcggcatta caaacagagt
    32761 ttggcgtcag ccgggttacg gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc
    32821 tcgaaagcat tcagggcagc gggacttacg tcaaagaaga gcgggtcaat tacgatattt
    32881 ttcagttaac cagttttgat gaaaaactgt cggaccgtca cgtcgataca cacagtgaag
    32941 ttctgatatt tgaagtgatt ccggctgacg attttcttca gcaacagcta caaatcaccg
    33001 tgcaggatcg tgtgtggcat gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc
    33061 tggaagaaac ctggatgccg cttgcgttgt tcccggatct cacctggcag gtcatggaaa
    33121 attcgaaata tcactttatt gaagaagtga agaagatggt tatcgatcgt agcgaacagg
    33181 aaatcattcc tctgatgcca accgaagaga tgagccgtct gcttaatatc agccagacaa
    33241 agccgattct tgaaaaagta tcgcgtggat atttagttga tggtcgggtg tttgaatata
    33301 gccgcaacgc ttttaatacc gatgactata aatttaccct gatagcgcag cgaaaatcat
    33361 cgcgataagc acaaaaaggc catcatacga tggccaacca tgtcgaaacc gacagccctt
    33421 cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat ttatttcaga
    33481 acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac gcctgcggct
    33541 tccagagcag cgaatttctc atccgcagtc cctttcccac cggcaatgat cgcacccgcg
    33601 tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacccac aactggcttg
    33661 gtaacatgct ctttgatgta cgctgccgct tcttcttcag cgctaccgcc gatctcaccg
    33721 atcatcacga tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat gtcgataaag
    33781 ttagagccag ggatcgggtc accgccgata ccgacacagg tcgactgacc gaaaccgtaa
    33841 tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac gatacccact
    33901 ttacccggtt tgtgaatgtg acccggctgg ataccaattt tgcattcacc cggggtgata
    33961 acgcctgggc agttcgggcc gatcatacga acgcctgctt cgtccagttt cactttcacg
    34021 gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt gatgcctgca
    34081 tcgatagctt ccagaatgga gtctttgcag aacggtgctg gtacgtaaat aacagacgcg
    34141 gaagcgccag tagcagcaac agcttcacgc acggtgttga acaccggcag gccgaggtgg
    34201 gtagtgccgc ctttacctgg agttacgcca ccaaccattt tagtgccgta tgcaatggcc
    34261 tgttctgagt ggaaagtccc ctggctaccg gtaaagccct ggcagataac cttggtgttt
    34321 ttatcaatta aaatggacat tatttcccct ccactgcggc aacaacctgc tgagctgcat
    34381 ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc ttcgcgccga
    34441 gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc acttccgcta
    34501 ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg aagatgttaa
    34561 ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt
    34621 ctttggttgc gccgccgcca acgtccagga agttagccgg ttcgccgccg tgcagtttaa
    34681 ctatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg atgttaccgt
    34741 ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg
    34801 actggtcacg catttcgcgc agatcaggct ggcggaacag ggcgttgcca tcagcgccca
    34861 gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg ttgatttcga
    34921 tcagcgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt ttggtgaact
    34981 gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc tgatacggca
    35041 tcgggccagt cagcggatca agcgcaactt tatggatcag gtgcggagtt tcttccgcca
    35101 ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacgcga cgggaactac
    35161 ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgccgct tcaaccagaa
    35221 tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc ttgcccagcc
    35281 agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca ccgcccgctt
    35341 taccgcggcc accagcgtga acctgacatt tcactaccca cggaccggca ccgatttttg
    35401 aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt gctggtaagc
    35461 catagcgggc aaacagttgt tttgcctgat attcatgtaa gttcatgtgt tctgtccatc
    35521 cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc ggtctacagt
    35581 gcaggtgaag cttaaactac tacacgtcca gcagcagacg cgtcggctct tccagtaact
    35641 ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg
    35701 acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc atcggacgat
    35761 ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca
    35821 tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa
    35881 ccgtcagctt gccatcacgg cctttgactg ccagctcttt gattttcttc tcgatgtctg
    35941 ccatgccgag ggtatcgaca tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa
    36001 ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt
    36061 tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc
    36121 ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa
    36181 tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca
    36241 gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg
    36301 cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct ttcgccgggg
    36361 ctttcgccag atgtttttcc acatcttcac gagtcagacg accacccaca ccggtgcctt
    36421 taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg
    36481 catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct
    36541 cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga
    36601 tctgacgaga cgttaccgtt gtaccttcat cttccagaac cgcatccaga atgccgtctg
    36661 ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac
    36721 gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt
    36781 caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac
    36841 gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac
    36901 cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa acggaatcac
    36961 ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca
    37021 ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg
    37081 gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg
    37141 ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt
    37201 cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc
    37261 gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca cgaccagcgg
    37321 acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg ccggggtaga
    37381 cggtacgcaa acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc
    37441 ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca ccagaccaca
    37501 catccggccc catttctgct cgccagagga gatgaactgg tcgataacca cctgcgcacc
    37561 gttggcgaag tcaccgaact gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc
    37621 ataaccatat tcaaacgcca gcacggcttc ttctgacagt acggagtccc agacacggaa
    37681 cgcgccctga ccgttatgga tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt
    37741 gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg
    37801 aaccggaatg ccttcatcaa ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc
    37861 gaacagtttc tcaccggctg ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga
    37921 ctgcatttca actgcttctg gtaccgtact gatacgttta gccagttcct gcaggcgctt
    37981 catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt
    38041 gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc
    38101 atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc
    38161 cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct gatacatcag
    38221 tggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcataccag
    38281 gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa
    38341 ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc
    38401 gatatcagta cagtacggtg tagaacgggc atccagcggg ttagaggtgg tgaaaccaac
    38461 ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat
    38521 gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat
    38581 tggcagcact ttgttgctgt tcggctcatc aagtctgtcc agacgggcac gaacagagcc
    38641 gataacgacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg ccaggtgcac
    38701 caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcgcctgt
    38761 gccgaggtgt tctttatgtt taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc
    38821 cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg
    38881 ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta acgcgtcacc
    38941 gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc
    39001 ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga aagtcgcgcg
    39061 accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtga tgtgcatata
    39121 ctcggcacca atcgggccgc agtaggtttg cttgagggcg gaaataagct cgccgagttt
    39181 catggtttct ttgccgctgg caaatgaacc gacgttgaag gtttcctgga agtctgcttc
    39241 ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag
    39301 cggatctaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac
    39361 tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc
    39421 tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc
    39481 cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt
    39541 taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga
    39601 gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg
    39661 cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag
    39721 ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca
    39781 gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggcctg
    39841 cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga
    39901 tgcaacgctt gcgcgtctta tcaggcctac agtttacgca ttacgttgca acaacatcga
    39961 cttgatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt
    40021 catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc
    40081 agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc
    40141 gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag
    40201 aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg
    40261 agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata
    40321 gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat
    40381 cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt
    40441 cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacggcagg agcggcggaa
    40501 cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg
    40561 accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt
    40621 atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat
    40681 cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga
    40741 ttccgactca ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa
    40801 gtcgaagcgg ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc
    40861 atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc
    40921 gctggaagtg tcatccagac gggcattttt cagacgctcg cggatcactt tcaactgctc
    40981 aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc
    41041 ttgcagcgct ttacggatcg ccaccggatc ttcaccgtta cgattattgt tccaccggtt
    41101 caggcgatcc agagacgctt caacatcaga ctcgctggca tcgtgcagtg cgccctgctc
    41161 ggcgatagac tcttgcagat gcagacctgc cgcgcgacca aagaccacca ggtccagcag
    41221 cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt caccaacggc
    41281 aaacagtccc ggaacaacca catcttcgcc tttctcattc acagtcagcg cctgaccggt
    41341 aactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg gaatcggctc
    41401 tttcaccgga tcgacgtgag cgaaggtacg ggaaagctcc aggatacccg gcagacggga
    41461 ttcgagaact tctttaccca ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc
    41521 atcacagccg cgaccttcac ggatttcaat catgatggaa cgcgccacca catcacggcc
    41581 cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag
    41641 cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc
    41701 ggtcgggtgg aattgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat
    41761 gccgacaccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa tacgccctgc
    41821 tccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac cggtttcgat
    41881 gcacagtgcg gtacaaccca ccaccgcgcc atcctggttt ttcaccagat ccagcgcata
    41941 ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag
    42001 tgcgtgaccg gtacggtcag cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt
    42061 tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcgagac gcgagaacgg
    42121 caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat
    42181 cgcgtcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat gccattccca
    42241 gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg
    42301 ggaacgggtc gggaagactt tagagagcag ggcacaggtc tggccgctct gggaaatttg
    42361 cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa attctctgac
    42421 tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt
    42481 gcaacgacaa tcaccagttg cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc
    42541 aacacctgcc acatgccgat ccaggcatga atcaagatag aaaacagcgc cagcagggtg
    42601 aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca
    42661 ctggtggcga aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg
    42721 cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata
    42781 cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc
    42841 gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac
    42901 ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga
    42961 agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca
    43021 gaagccactg caggatgccc actgcaacaa aggtgatcac accggaaacg cgatggagaa
    43081 tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt
    43141 gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg
    43201 actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg
    43261 tctgcttgcg ggtcagacag cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc
    43321 cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag
    43381 acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat atagtttgtc
    43441 gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa tttttaatca
    43501 tctaatttga caatcattca acaaagttgt tacaaacatt accaggaaaa gcatataatg
    43561 cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta
    43621 ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta
    43681 agtacttgat tcgccattta ttcgtcatca atggattctt tacctgcaag cgcccagagc
    43741 tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag
    43801 ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag
    43861 gagaccttaa atggctgata caaaagcaaa actcaacctc aacggggaca cagctgttga
    43921 actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc
    43981 aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat
    44041 tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct
    44101 ggcgaccgat tctaactacc tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac
    44161 tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca
    44221 gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg
    44281 tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca
    44341 tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta
    44401 caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa
    44461 cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga
    44521 acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc
    44581 caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat
    44641 tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga
    44701 agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga
    44761 ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac
    44821 cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct
    44881 ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa
    44941 actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc
    45001 ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag
    45061 cgaaatgcac agttacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga
    45121 aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt
    45181 ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa aaattgtaaa
    45241 aataaaatcc actatcggac attcatatat tctaaaaaaa aatacatcgg tataaattat
    45301 aaacaaatag atgattttta atcatctatc ttcctctcag ttacatgaat aaaccaccaa
    45361 atatagcgca cccctataat tcccaatact ttattcatca aacatttaat tcataccatc
    45421 aattaattac ttttacattt aataaaatta ataatatttt taagaaaaac taaaaacatt
    45481 cgacaatcaa ataagatatt cttgcaccga tatacttttt tcttttctgt taaaggactc
    45541 agttatgttt aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcacagc
    45601 acctgtttat gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga
    45661 agcaccgtgt gaaatacatc aggatgatat tgataaagag gttgaactcg gtcaggtgac
    45721 caccagccac attaatcagt cacatcacag cgatgccgtt gctgtcgacc tgctcttagt
    45781 caactgtgat ctggaaaact ccagcaacgg ttccggtggc aagatttcta aagttgcagt
    45841 cacctttgat agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac
    45901 gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa gatcaaagca atatcgtttt
    45961 aggcaccgct actccagata tcgacctggc tccgacctcc agcgaacaaa cgctgaattt
    46021 ctttgcctgg atggaacaaa ttgatcaggc tacacctgta acgccaggcg cagttacggc
    46081 gaatgcaacc tacgtgctgg attataaata agtattactt tggcggcgct atcgccgcct
    46141 ttcataaaat atttatcagg tatggacacc gtgaatattt atcgactctc ttttgtatcc
    46201 tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat
    46261 aaatcaatgc gcgaccgcat tgatatttca ttattaaagg aaaaaggagt cattgctccc
    46321 ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa tcagtaacga gcaaaagatt
    46381 aactggcaca aaaatgacga taaaaccatt ccgtgcatca atgatttact ggtcgataaa
    46441 tttggcttaa aacctgaagt ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt
    46501 agctcccgac ctgaaatgct cttcaatttc gatcaagcca atcagcaact gaatattagt
    46561 attccgcaag cctggctggc gtggcactca gaaaactgga ccccaccctc cacatggaaa
    46621 gaaggtgtcg ccggtgtcct gatggattac aacttgtttg ccagcagcta ccgcccacag
    46681 gatggcagca gcagcactaa tcttaacgcc tatggaacag ccggaatcaa cacgggggca
    46741 tggcgcttac gcagtgatta tcagttgaat catactgata gcgatgataa ccatgaacag
    46801 tcaggcgaaa tatcacgtac ctatcttttt cgcccgttac cgcaattagg ttcaaaatta
    46861 accctcggcg aaacggattt tagtcccaat attttcgacg gtttttctta taccggcgcg
    46921 gcactggcaa gtgacgatcg aatgttgcca tgggaactac gcggctacgc cccacaaatt
    46981 agcggtattg cacagaccaa tgccacggtg acgatcagtc aatcaggccg cgtcatttac
    47041 cagaaaaaag tcccaccagg cccatttatc attgacgacc ttaatcagtc tgttcagggc
    47101 acactggatg tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcagca
    47161 gcatcgacgc ccttcctgac tcgtcaggga caggttcgct ataaactggc cgcgggtcag
    47221 ccacggccct ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc
    47281 tgggggatgc tgtcaaacac ctcgctgtac agcggcctgc tgctttctgg tgatgactac
    47341 cattctgccg caatgggtat tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat
    47401 gtcacgtggg ccagtagcca ttttgatact cagcaggacg agcggggctt aagctaccgt
    47461 tttaattaca gcaaacaagt ggatgccact aacagcacga tttccctcgc cgcttatcgt
    47521 ttctccgatc gtcattttca cagctacgcc aactatctgg atcacaaata caacgacagc
    47581 gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat taccccacta
    47641 aacctcaatc tttacgccaa cctgctacat caaacctggt ggaatgcaga cgcctccacg
    47701 accgccaaca tcacggcagg ttttaatgtt gatattggtg actggagaga tatctctatt
    47761 tctacgtcat tcaatacaac ccattacgaa gataaagatc gcgacaacca gatttacctg
    47821 tcgatttcgc tccccttcgg taacggtggt cgggttggct atgacatgca aaacagtagc
    47881 cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa tagctggggc
    47941 atgtctgccg gactgcaatc cgaccgtcct gacaatggag cccaggtgag cggtaactat
    48001 cagcacctga gttcagcggg tgagtgggat atttctggta cctatgccgc caatgattac
    48061 agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt
    48121 catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat
    48181 attccggttc agggcaatct cgactacacc aaccattttg gcattgcggt ggtgccgttg
    48241 atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc cgacggcgta
    48301 acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg ttacaaatca
    48361 ctggcttccc gttccggtaa agacgttaac gtcatcattc gcaacgccag cggtcagttt
    48421 cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg gatggttggc
    48481 gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg
    48541 ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac cgcaaatcgc
    48601 ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa ggactacccc
    48661 ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat cgcactcgta
    48721 ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt agcgataaac
    48781 tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc
    48841 tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacacag gtacggatag
    48901 taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt ttctacaata
    48961 tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg caagtcgcca
    49021 ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa
    49081 aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg aaaaatccta
    49141 ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt ctccccggct
    49201 tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga aattatagcg
    49261 gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg acgacgctca
    49321 actgcagcgg acaatgccat ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg
    49381 agtgttgctc gtcatctgtc tgttatttct gtcgttgaag tctgcgcttg ccctgaactg
    49441 ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac cttttgctgt
    49501 accaggcaat gccaaactcg gcgataagat ctgggaatct gacgatatta aaataagatc
    49561 tgggaatctg acgatattaa aattcccgtc tattgtgaca acaataccaa cggcaacttt
    49621 gaaagcgaac atgtctacgc atgggtaaac ccctacccgg gggtgcagga tcgttattat
    49681 caactcggag taacttacaa tggcgttgat tatgacgcca accagggaaa aagccgcatc
    49741 gacactaacc agtgtatcga cagtaagaac attgacattt atacccctga tgggatggca
    49801 gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcgcgaacct ttctcgctcg
    49861 catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa gtacgcttag
    49921 cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagacc ctactgccca
    49981 aaacctgaaa tatcatatta ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa
    50041 tttttccatt tcaccggaaa cacagatgat tgattttggt aaatttaatt tgctggatat
    50101 tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca
    50161 atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt tggttgaaga
    50221 agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg aaaacgcctc
    50281 gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta
    50341 cgaaaaaacc tatacagctg aactttcgtc cattgcaggt acccccatcg aggctgggcc
    50401 cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc
    50461 agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct tgattgctgt
    50521 tattttttcc tagcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga
    50581 gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt
    50641 ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg
    50701 ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg
    50761 cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc
    50821 tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca
    50881 tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt
    50941 cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc
    51001 gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg
    51061 tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc
    51121 cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg
    51181 gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc
    51241 tccagtctgg tcagctcatc accatcgaac taccggaatg gctgctggcg atggcgtata
    51301 tggcaattgg ctggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcactac
    51361 gcccgctacc gcaaatcctg ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg
    51421 cgtggggact gacccggttt atgcatattg attttatgac tgcctacctc gccaccagtc
    51481 ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta
    51541 ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacggggcca gccattgcgc
    51601 ggtttatttc aacctatgcg ccgaagcggt cggcctagtg ctggcagcct ggacaccagt
    51661 aaaacgggcg cgaggacaac gtggttttct caatgatgcc gccacagcgt tcgcacagtt
    51721 cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca
    51781 cctgcccccg cgtagcgtaa gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat
    51841 ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga
    51901 ggatctccac ccgcaaataa ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc
    51961 ctgcaaactg acggttacga aagcgcggcg acaataaccg ttcctttacc acctccggcg
    52021 tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt
    52081 gttccggcct caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg
    52141 tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc
    52201 gccagacgcc gtaaagctga ttatggctgt agagcgttaa accgttggga aaatgagtta
    52261 acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt tgtgattgat
    52321 aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg
    52381 cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt
    52441 ttcattcttt attaagcggg gttatgctgc gacaacaatc ccctttttgg caaatgtagc
    52501 tcgtaatcgg cgggcgaacg ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg
    52561 cgccgcgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc
    52621 cagcgtttgc gccaacgcct gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg
    52681 caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag
    52741 gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaaccaatgc
    52801 cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc
    52861 ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc
    52921 aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt ccggcggcag
    52981 ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc
    53041 gacaccattt tttattgctt cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg
    53101 aaatccacaa gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca
    53161 gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac gctaattgtt
    53221 caaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa
    53281 aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta catatcagcc tcaatgatac
    53341 aggcaatacg tggataacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc
    53401 cgttatgcgg cacctgcacc acgctcggta acaaaccgtg agataacagt tcgcgatcgg
    53461 tgatgcgttt taaaatttgc ccctgtaaac gatagcccat tcggttactt tgcgagctaa
    53521 gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg
    53581 gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca
    53641 taaaatcacg cttcgatttg ccaatcggga gtcagtcacc atccttcagt aaacggcctt
    53701 ccagcccgcc aatccccact ttgagatcgg tgctgcatga ccccattacc ggcggaacat
    53761 caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta
    53821 aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcgtta tcatccaacc
    53881 gcgcttcgca acctgcaccc gtcagggcaa accacccatc agtttcgaac tcaactgtta
    53941 actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc agcaggttag
    54001 caatgcgcag cgcaggcata tccagtgcgc cacagtggct gatacccgac tggcaaaaac
    54061 cgtgacgacc gccatcctgc acagtggtgt acatgcccgc acgaataatc ttcagcatat
    54121 tccctccttc tgcggtacaa atcgcacgct gtctcccgga cgtaataaga tgggttcgtc
    54181 acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccagc cacccggcgt
    54241 tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag
    54301 caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg
    54361 gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac
    54421 aacctgtttt tcgctcaacc cgcaatacgc cgcgaccacc gccaaatccg gtccgcctgc
    54481 accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc
    54541 ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag gattacgcaa
    54601 aatcaccgtg atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag
    54661 acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc
    54721 cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc
    54781 tgattattcg ttatgcagga ttaggaatat caataaaggt cacatcaaga tcggtatttt
    54841 cattcagcca ctcgctcaat gcgcgaatac caccgcgttc agtggcatgg tgacctgcag
    54901 cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag
    54961 taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg
    55021 tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac
    55081 gtcccagacg cgcttcaatc caggaggcta gttccattcc cggcacaggc atggtcagct
    55141 ctccccacgg caccaacggc tcaatttcgc ccatgaccgt gatccccagt aacgccgcca
    55201 gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt
    55261 tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag
    55321 actctccttt ccagaagtag ccatgatgca caatgaccgc atctgcgccc agacgcactg
    55381 cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt
    55441 tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt
    55501 cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc
    55561 tacaccgtta acatagcgtt acatcgcctg ttcgtcgata gccaatcttt tgacatatta
    55621 agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt
    55681 atatttatgc aatcagacag catattgcat aaataatcag atctgtatca aagttttaac
    55741 tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat
    55801 tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat
    55861 ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca
    55921 actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta
    55981 tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt
    56041 gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca
    56101 tccgtcattc ctctatctgt ccctggcgat tatcgtctgc ggctatggcc tgtttaaatc
    56161 taacgtaagc tgtctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg
    56221 tttctcgctg atgtatgcgg cgggtaacgt gggatctatt atcgcaccta tcgcctgtgg
    56281 ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg ttggcatgat
    56341 cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc actcataccc gcggcgttaa
    56401 caaaaaagta ctgcgtgcga caaactttct cctgccgaac tggggatggc tgctggttct
    56461 gctggtggca acgcctgcac tgattaccgt gctgttctgg aaagagtggt cggtatacgc
    56521 cttaattgtc gcgactatta ttggtctggg tgtactggca aaaatttatc gcaaagcaga
    56581 aaaccagaaa cagcggaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt
    56641 gttctgggcc ttcgcacaac agggcggtag ctcgattagc ctttatatcg accgcttcgt
    56701 taaccgcgat atgtttggtt ataccgttcc gaccgcgatg ttccagtcga ttaatgcctt
    56761 cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa
    56821 tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt ggcctgatga gcgccggatt
    56881 ctgcattctg accttaagcg cccgctggtc cgcaatgtat ggtcactctt ctctgccact
    56941 gatggtgtta ggcctcgcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat
    57001 gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc gtattaaccg gcatctacat
    57061 gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg attgccgatc agacatcgca
    57121 ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt
    57181 tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta
    57241 tcaggcgctg aaattcagaa accgcgcgct ggcgctggag tcataatagg ccagcaccga
    57301 tgcctgatgc gacgcttgac gcgtcttatc aggcctacgt atttcctgca atttattgaa
    57361 tttgcacaaa tttgtaggct ggattaggcg ttcacgccgc atccggcatc tgggctcgat
    57421 tgcctgacgc gtctgttatt tccccttccg cgccgcctca tacgctgcca acgtttgtac
    57481 tctcgcctct ttgtgctcga ctatcggttg cggataatcc agcttcacac ctgctttctg
    57541 cgcccacttc cacggctcat gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca
    57601 tcgacgaata aactcgccct cacggtcaaa tttctcgccc tgagttatcg gattgaaaat
    57661 acgaaaatac ggcgcagcat cagtacccgt agaagccgcc cactgccagc caccgttatt
    57721 agctgccaaa tcaccatcaa tcagctgcga catgaaatat cgctcgcctt cgcgccagtc
    57781 gatcaacaaa tctttcacca gaaaactggc tgtaatcatc cgtagccggt tatgcatcca
    57841 gccagtgctg ttaagctgac gcatagcggc atcaataatc gggtatcccg ttttgccttt
    57901 ctgccaggcc tgtaaatgtg cgggattgct ttgccactgc acacgatccg tccaggcaat
    57961 aaatggacga tgtttacaca acgaggggta atacgtcatc agatgacggt agaactcgcg
    58021 ccagatcagc tcactaagcc agacactacc ggccccaccg tccagcgcct gcggctgttc
    58081 cgccaacaag cgatgcaaac actggcgagg cgataacccg cccgttgcca agctggcgga
    58141 caaacggctg gtgccttcca ctgccggaaa atctcgttgt tgctcatact ctccagcacc
    58201 gttctcgcaa aactggcgta attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc
    58261 agtatcgaaa gactgacgag gataattcag cgtaatggat ggcgcgggct ttatcgatcc
    58321 gctactacga acttttggtg cagcgacgca ctccggcatc ccttcccgca gccgtttcag
    58381 ccaggcattc ttaaaaggcg taaagacttt gtacatctcg tgattgccgg tcatcaccgc
    58441 ccccggcggc aggatcacgc tgtcatcaaa tccttcacac accacgttac gcagcgctct
    58501 ttcaacgtcc acatcccgcg ctcgctcatt cacctcatac tgatagttat aaaaaagatg
    58561 agtaacgctg ttttccgcgc acacctgttt aactatttcg acactggcga caaagtcatc
    58621 cacttcacgg aacaataaag gaataccttt ttccgccagc gcgatttgta gcccattcag
    58681 ttgagcattg ataagttcag cctgacgcgg cgacatgtta tgcgtctccc actggcgtgg
    58741 tgtagcgata tacagcgcca gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc
    58801 gagattatcg tgcagacgta aatcctggcg aaaccaggcc agatgggtag tcataaaact
    58861 cctgaatggc gcaagttaat cacacgcggc caaagttcga aataacgctg ccctgaaagc
    58921 caggcgtcag gataaagcgc catatagtgt ttgatacgca tcagcggcgc aagaagtggt
    58981 tgctcgccac ggcgataact gtcgataagc gcagccagct gctggcgttc tgtggaatca
    59041 atatgcgggc gaaaataacc ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg
    59101 gcgggatgag aaagcaaaac aattacgcgt tggcggtact ggttgtaaaa atcatccaga
    59161 ttttgccact gatgaatatc agccacaaac gggccaagtt tgcggtactc gggctgagaa
    59221 tgggccagaa agacgagttt atagcggctg tgataatcga gaagcgcccc gcgcgttaag
    59281 ccatgttctt tcagtcgatg taactcatga agggcgaaga tacggattag ggtgctgtca
    59341 tcaaatcgtt gtagattcat tactgctcct gaacggtata caggagtaag tgtaggcgtt
    59401 ggtaaatgat gtttgtgaag ttaatccgct agcagggcaa gattacgaaa gcgcgctccc
    59461 cgcaagcaac taatcaaggt agttaatcat ggctgaacac gatattacgc cagaagtcac
    59521 catttccaaa acacagcgcc gctataaagt cggctatgtc agagcccgcc accaggaccg
    59581 caaaaccggt atagcccggt attattcaca acatcccagc ctgcacctta agggcgactg
    59641 gctgaaagag gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg
    59701 cctgaccatc atcgctgaca gtgacgaggt gcaggaactg cgggatggga tgtttagtgt
    59761 gctgaacgaa agttaaatat taaaaaagct gggaagattt aaaaccttcc tagcttttta
    59821 ttctgtctta ttataatgga tactcatcat cttccgatga atttataaat tgctccaagt
    59881 ttacatgaag agtacttaac ctagttttta actcattaag tgaagcgata atttcatatt
    59941 ttccaacata atcatatgat ggatcgtatt cattatctat gtcagaagct aaaaatgatg
    60001 atattcgtag tttaaattta gcttcatctt tattacattc attggcataa ggtgaatgcc
    60061 ctatcatagc atattcatct aatctaaaag gataagatgg ttttaattca tcgatcaatg
    60121 aaatttcaat ccatgttcta tatccaggca ttaatggact aatttctttg agcaaaacct
    60181 ttccacttaa taatgtattg agattgttca ttattatgtc tcccacggga aaaatatatg
    60241 tctaacactt ccaccatgtt gatgaacttg taaatgtggt aaatcaccat caggatttcc
    60301 tagtccatga cctgacactc tatatgaagg ttttgttaca gaaggatctt ttatatcatc
    60361 ccaatgatat gaacctgctt ttgttttatt tccagtaaga tattccatta agttcttagt
    60421 gctggggcct gactcacctg tcatattttt ataattatga cctaaatatc tatccataaa
    60481 caattgttca gcgtcacttc tagacctaac agtaactttg gtttcactac tatctaataa
    60541 acgattcgct ttttggttac taacagcaca tggagttaat ccaagtggat cagcatattt
    60601 tagaggatta ggtgcataac tgtagagatt taaaccgcct ctcaacccta tcgggtcagt
    60661 taccatgaac cgcccgacat ccggatcata gtacctgtgc agattgtagt gcagccccgt
    60721 ttccctgtcg aggtactgtc cctggtagcg caggttctgc tccaccccgc cgtggtcctt
    60781 ctcctgtatc gtgttacccc agacctgata ccccgtctcc cacacgattt tcccgtcgct
    60841 gtccgtcatc tcttccggcg cgccgttcac atccgtgtga aaatacagta tccggttcgg
    60901 gccctctttg cccgccttat ccacccgcgc cagcggttca taactcccct ggtccctgta
    60961 gatgtacagg ctgcctgttc cctccggcct gctctcctgc gccagccgca ttccgtccca
    61021 taaaaatttt gtccggttgt acggcttacc gtcagggctg atgtgctgtt tttccgtccg
    61081 cctccccagt gcatcgtaca cataccggta tgtcccccgc gctgtcgtca cttccaccat
    61141 ccggtgttcc gcatcataac ggtaactctg cgttccgctc cggccctcct tttccaccgc
    61201 acggccaaat tcgtcataac ggtagtgcac accacggtaa gacatcagcc ggttgaacgg
    61261 caccacgctg ttttctgccg tggccctttc ccccgcgcgc ctccccagca ggtttcccgc
    61321 cgcgtcgtac tgccagctcg ccagataccc ctcatcctcc gaacgacacg ccgtgatgcg
    61381 ccccgtgggg tcgtagaagt aatccgtttc cccccgccgg ctgtgccgcg tcttcagcag
    61441 ttcatcctcg ccactgtagc tgtactgccg gtccgtgaac gtctccggta ccaccccgcg
    61501 catcccgctg tatgtctccc tgcgcttcag ccgcccgccg gggtcgtaaa gctgccgcgt
    61561 gctcagtcgc ccctggcttc tgagcacttc acggtgcagg tggtcgcgcg tgaactcgct
    61621 gatgacctcc cggtccagat ttatctgctg gagatggccg ctgccgtagt acagatggcg
    61681 ctgcgttctg ccgtccggaa aggtggtggc agtccggttg cccagtttat cgtactggtg
    61741 ggtcaggatg ccctgcgccg tctcctcgct cagcatccgc cccagtctgt cgtagctgaa
    61801 gcggataact tccggcgcgt cagtttcgtg ccgctggcga cggatttcca gtagctggtc
    61861 tgccgcatca tggacgtatt ccgtacggcc atcggctgtc tcttttgccg tgagccggcc
    61921 cgccgcatca cgcgccagcc ggtggcggat ttcaccgccc cggtccccgg tgaacaccac
    61981 ttccgtgacc gcgttcagcg cgttgtagcg gtacgactgg cggcagccgt ccgggtttat
    62041 ctgctccgcc agacggtcca gcacatcatg gcggaaccgg tagcgttctc cgttctcgtt
    62101 cgtgagcgtg accagacgcc cgtaagcgtc gtacccgaat gccgtcctgc gccccatcgc
    62161 gtccgtccgg ctgcgcaccc ggccctgccc gtcccgctgc cagcgcgtta tcctccccat
    62221 cgggctggtg tactttacca gcctgcccgc cgcgtctgac tggtaattca ccgtgcggcc
    62281 gtccgccagt atcacctccg tcagatgacc gctgtcgtca tacccgtagc gcgtgctgtg
    62341 gctctgcgca tccgtcattc tgataagcct cgtgcgttcg tcgtagaacc acgccgtctg
    62401 cgagccggaa cagtccgtgt gccgcatgag ctgcccgtct tcattccact ggaggtattt
    62461 attccccccg cgcgcatccg ttatctgcac cacctagccg tggtggtcat actgataccg
    62521 ggtgttctgc tgcagcgggt cggtcacggc aagaaggttg ccgcgttcat cgtacacaca
    62581 gcgccaggtg tttcccgccg cgtccacctc cgtttccggc tggtgccaca ccgggtgcca
    62641 ctgcgcctgc tccacccgcc ccagcgggtc gtgcgtctcc gtgatatgcc cctgcaggtc
    62701 atacacatag cgccatttgc cgccctgagg gtcggtcatc cccagcaaca gtcgttcttc
    62761 gtcgctccag cggaatgtcg tcacctgtcc ggcttcgtcc tggtacgccg tcacctgatt
    62821 ctgcgcatcc cactggtgat aacgtgtgcg ccccagtccg tccgtcacgc acagcctgcg
    62881 ctgcgccagg tcccacgcca gccggtactg ttcaccgctg tccgtgtggt gccccgtcac
    62941 ccgccatgcc gcgtcatccg gaccttcata ccagccccac tggtaataat acccttcgcc
    63001 gcccggctgg cggtgcagcg tcatgcagtg gttttcgtcg taagcaaact cacgcagcag
    63061 gtgccccgtg ctgtcgtaca cgccgttcag gtggccggcc tcgtcatagc tgtaacgacg
    63121 cagcagtaaa ggtgcagtct cagggaaatg ctgataaatc tgtgtcaccc gccggggatg
    63181 cgccgccagt tcgtaataca gacggatgca cggacgctgc tgttcgccgc tgatttccac
    63241 aatgcggccc cggtcgtcgt agtacagatt cagacagttg ctgttacggt cgcccagcat
    63301 tttcagccgc tggcgctgcg ggtggtgcgg gtcagcctca aacagaaaac actgcccgtc
    63361 atcgctgctg ataagccagt gcccctgctc accacgcctt accgccagcc cttcaccggg
    63421 gctgtaaaac ccgccccccg gcagtacggc ttccatatcc aagcgtcgcc ccatgcccga
    63481 cacaaaggtc atgcagtttt cgtctgcgct acccggtgtg cacacgaggc tgactttata
    63541 cattacactc cagccctgac caaacattcc ttccgtacgt tcgtcacgac tgctgtacac
    63601 gcgctgccag tccagcggaa agtggcccgg cagactgaaa tcgaagtcat cgtcatcggc
    63661 caggtatttg gccccggtgg cggcattgac cggatgcgga agggcactgg tggccttctg
    63721 cgtcagcatg ctcatcccga cactggcagc aaaacaggcg attttagaaa gcattttgcc
    63781 cgggcgaaga aacatcagcg ccacggttgc caccagggtt atctgcgacc tgccgcttct
    63841 gatatccctg accaccagta acggaccgcc aatgcgcaca tccggtgaca caaacgcccc
    63901 gttccccggc tcattcacca ccctggcctc gcaggtacag cgtgccccgc tgcgtgtcgc
    63961 cggctggctg ttgatacgca catcccgcgc gccttccgcg atatattccg ctgcgggcgg
    64021 ctcgtcatcg cctttgaaca gattactgat gctgccaata accgcattca ctccctgaaa
    64081 ggcggtttcc agcataccgc ccggattcag caggttaagg gccgttccca gggggtcatc
    64141 cagaaaggcg gctttttttt gtgcgagaaa ctgcgtcagt gaggaaggat gtttatggca
    64201 gtcgatttta tcgtcagggg tcggggtgga tcccggggcc gcactggcca cagtgggctg
    64261 ccatagttcc gacacaaaat gtgtcacgcc atccaccatt tcagaacgcc cttcttccgt
    64321 actgagctgg tcgtgcagag cccgggacat cagcacaggc cctgtcaccg ggttaaccgc
    64381 cagggctatg gcccccgcca tttccagata ttccccggcc acatcccgga acgttctggc
    64441 atcctcctga cgctgcgctt cttcctgttc tgccttcgcc tgcgcttcct gcgcctctat
    64501 gtcctgccgc gatgccgcag tcgccgctgc ccgcgcggca ggtttgctgt tgatccatgt
    64561 gtcaccagag ccggtgagga tgtaaccttc aatctgaggc gggaaaagca tatttgcgag
    64621 ggctgaacag gcttcgctga ccttttctgt cacgccggtg tacaccatcg ccacacccgc
    64681 cacaaaaccg gtcagcacga cggccgtgca ccccatcgtg atccccactg acgtcgccag
    64741 taacgaggtt gtgccgacaa tgagggcatc aataacgaca tttgcggcca cctccagcac
    64801 cgcccccagc acatctgcca taaacgacgt atgcagcagg gcatcaccct gtcttgccgc
    64861 ccagaatgcc tcttccatgt cccgttccct gtatgattaa ttcgtcctga cttcctgccg
    64921 catcctgaag cgcagattct ttttaatttc agcccagcgc gtcatatctg ccggggtcag
    64981 cggtgtggtt ctggcgtagg tgaaggccat catgacccgc tcacccggca atacaaatgc
    65041 cacctgacac tggtacacct gctcgttacc ccggagaaac tggctctgta tttccaaacc
    65101 gcctcgttga gataatattc cgtcataatg taaatagtcc gttttaataa aggaattatt
    65161 tattgacagg ttctgttttt ttaatcatga atgtaatacc catcaccgct ttacagcaca
    65221 ctttatcttt ccagccataa tcactaataa tattaagcaa ttagcaatat ttacaacgac
    65281 cagagcggat acatagttat gtatttaaca atcccaatat attcactatg caaataaaac
    65341 cactagtaac agacacgcta ccgcctccat ataaaaatgg acaccttata aaattgtatc
    65401 ttgcatagaa aaagctgaaa tatcggatgc gttttaatta tcaactataa atctataagc
    65461 tttttattaa caa
//
LOCUS       DAJTNW010000012        60413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNW010000012 DAJTNW010000000
VERSION     DAJTNW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..316
                     /locus_tag="OO179_000795"
     CDS             <1..316
                     /locus_tag="OO179_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5530961.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO179_000796"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO179_000796"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5530962.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO179_000797"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO179_000797"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5530963.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO179_000798"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO179_000798"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5530964.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO179_000799"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO179_000799"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5530965.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO179_000800"
     CDS             complement(3720..4949)
                     /locus_tag="OO179_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5530966.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO179_000801"
     CDS             complement(5013..5453)
                     /locus_tag="OO179_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5530967.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO179_000802"
     CDS             complement(5558..6328)
                     /locus_tag="OO179_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5530968.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO179_000803"
     CDS             complement(6325..7251)
                     /locus_tag="OO179_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5530969.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVEHKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO179_000804"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO179_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5530970.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO179_000805"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO179_000805"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5530971.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO179_000806"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO179_000806"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5530972.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO179_000807"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO179_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5530973.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO179_000808"
     CDS             12958..13305
                     /locus_tag="OO179_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5530974.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO179_000809"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO179_000809"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5530975.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO179_000810"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO179_000810"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5530976.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO179_000811"
     CDS             complement(15125..15487)
                     /locus_tag="OO179_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5530977.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO179_000812"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO179_000812"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5530978.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO179_000813"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO179_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO179_000814"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO179_000814"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5530979.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO179_000815"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO179_000815"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5530980.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO179_000816"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO179_000816"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5530981.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO179_000817"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO179_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5530982.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OO179_000818"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OO179_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5530983.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO179_000819"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO179_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5530984.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO179_000820"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO179_000820"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5530985.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OO179_000821"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OO179_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5530986.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OO179_000822"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OO179_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5530987.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OO179_000823"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OO179_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO179_000824"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO179_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5530988.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO179_000825"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO179_000825"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5530989.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO179_000826"
     CDS             complement(36342..36380)
                     /locus_tag="OO179_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5530990.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO179_000827"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO179_000827"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5530991.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO179_000828"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO179_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5530992.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO179_000829"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO179_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5530993.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO179_000830"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO179_000830"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5530994.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO179_000831"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO179_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5530995.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO179_000832"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO179_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5530996.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO179_000833"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO179_000833"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5530997.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO179_000834"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO179_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5530998.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO179_000835"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO179_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5530999.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO179_000836"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO179_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5531000.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO179_000837"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO179_000837"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5531001.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO179_000838"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO179_000838"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5531002.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO179_000839"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO179_000839"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5531003.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO179_000840"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO179_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5531004.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO179_000841"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO179_000841"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5531005.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO179_000842"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO179_000842"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5531006.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO179_000843"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO179_000843"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5531007.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO179_000844"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO179_000844"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5531008.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO179_000845"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO179_000845"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5531009.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO179_000846"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO179_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5531010.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO179_000847"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO179_000847"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5531011.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO179_000848"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO179_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5531012.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14474 a        16732 c        14844 g        14363 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgtgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgaacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacttcctt gatgtaaata aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact ggacgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga attgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcaataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtagag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatacg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacagctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgac tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAJTNW010000013        59735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNW010000013 DAJTNW010000000
VERSION     DAJTNW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OO179_000849"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OO179_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5531013.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OO179_000850"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OO179_000850"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5531014.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OO179_000851"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OO179_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5531015.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO179_000852"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO179_000852"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5531016.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO179_000853"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO179_000853"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5531017.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OO179_000854"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OO179_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5531018.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OO179_000855"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OO179_000855"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5531019.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OO179_000856"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OO179_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OO179_000857"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OO179_000857"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5531020.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OO179_000858"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OO179_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5531021.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO179_000859"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO179_000859"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5531022.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OO179_000860"
     CDS             complement(13538..13933)
                     /locus_tag="OO179_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5531023.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO179_000861"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO179_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5531024.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OO179_000862"
     CDS             complement(15220..15447)
                     /locus_tag="OO179_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5531025.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OO179_000863"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OO179_000863"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5531026.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OO179_000864"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OO179_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5531027.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO179_000865"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO179_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5531028.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OO179_000866"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OO179_000866"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5531029.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="OO179_000867"
     tRNA            19689..19765
                     /locus_tag="OO179_000867"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            complement(19840..21093)
                     /gene="amiC"
                     /locus_tag="OO179_000868"
     CDS             complement(19840..21093)
                     /gene="amiC"
                     /locus_tag="OO179_000868"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5531030.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21325..22656
                     /gene="argA"
                     /locus_tag="OO179_000869"
     CDS             21325..22656
                     /gene="argA"
                     /locus_tag="OO179_000869"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5531031.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22718..24544)
                     /gene="recD"
                     /locus_tag="OO179_000870"
     CDS             complement(22718..24544)
                     /gene="recD"
                     /locus_tag="OO179_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5531032.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24544..28086)
                     /gene="recB"
                     /locus_tag="OO179_000871"
     CDS             complement(24544..28086)
                     /gene="recB"
                     /locus_tag="OO179_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5531033.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28079..30967)
                     /gene="ptrA"
                     /locus_tag="OO179_000872"
     CDS             complement(28079..30967)
                     /gene="ptrA"
                     /locus_tag="OO179_000872"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5531034.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31143..34511)
                     /gene="recC"
                     /locus_tag="OO179_000873"
     CDS             complement(31143..34511)
                     /gene="recC"
                     /locus_tag="OO179_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5531035.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34524..34856)
                     /locus_tag="OO179_000874"
     CDS             complement(34524..34856)
                     /locus_tag="OO179_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5531036.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34841..35248)
                     /locus_tag="OO179_000875"
     CDS             complement(34841..35248)
                     /locus_tag="OO179_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5531037.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35245..35808)
                     /locus_tag="OO179_000876"
     CDS             complement(35245..35808)
                     /locus_tag="OO179_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5531038.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35799..36269)
                     /locus_tag="OO179_000877"
                     /pseudo
     CDS             complement(35799..36269)
                     /locus_tag="OO179_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36453..37247)
                     /gene="thyA"
                     /locus_tag="OO179_000878"
     CDS             complement(36453..37247)
                     /gene="thyA"
                     /locus_tag="OO179_000878"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5531039.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37254..38129)
                     /gene="lgt"
                     /locus_tag="OO179_000879"
     CDS             complement(37254..38129)
                     /gene="lgt"
                     /locus_tag="OO179_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5531040.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38280..40526)
                     /gene="ptsP"
                     /locus_tag="OO179_000880"
     CDS             complement(38280..40526)
                     /gene="ptsP"
                     /locus_tag="OO179_000880"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5531041.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40539..41069)
                     /gene="rppH"
                     /locus_tag="OO179_000881"
     CDS             complement(40539..41069)
                     /gene="rppH"
                     /locus_tag="OO179_000881"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5531042.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41754..42443
                     /gene="mutH"
                     /locus_tag="OO179_000882"
     CDS             41754..42443
                     /gene="mutH"
                     /locus_tag="OO179_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5531043.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42512..43225
                     /locus_tag="OO179_000883"
     CDS             42512..43225
                     /locus_tag="OO179_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5531044.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43363..43581
                     /gene="ygdR"
                     /locus_tag="OO179_000884"
     CDS             43363..43581
                     /gene="ygdR"
                     /locus_tag="OO179_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5531045.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43689..44729
                     /locus_tag="OO179_000885"
     CDS             43689..44729
                     /locus_tag="OO179_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5531046.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44762..45952)
                     /gene="lplT"
                     /locus_tag="OO179_000886"
     CDS             complement(44762..45952)
                     /gene="lplT"
                     /locus_tag="OO179_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5531047.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45945..48104)
                     /gene="aas"
                     /locus_tag="OO179_000887"
     CDS             complement(45945..48104)
                     /gene="aas"
                     /locus_tag="OO179_000887"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5531048.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48690..49721
                     /gene="galR"
                     /locus_tag="OO179_000888"
     CDS             48690..49721
                     /gene="galR"
                     /locus_tag="OO179_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5531049.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49728..50990)
                     /gene="lysA"
                     /locus_tag="OO179_000889"
     CDS             complement(49728..50990)
                     /gene="lysA"
                     /locus_tag="OO179_000889"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5531050.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51112..52047
                     /gene="lysR"
                     /locus_tag="OO179_000890"
     CDS             51112..52047
                     /gene="lysR"
                     /locus_tag="OO179_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5531051.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52034..52726)
                     /gene="ygeA"
                     /locus_tag="OO179_000891"
     CDS             complement(52034..52726)
                     /gene="ygeA"
                     /locus_tag="OO179_000891"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5531052.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52855..54273)
                     /gene="araE"
                     /locus_tag="OO179_000892"
     CDS             complement(52855..54273)
                     /gene="araE"
                     /locus_tag="OO179_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5531053.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54588..55349)
                     /gene="kduD"
                     /locus_tag="OO179_000893"
     CDS             complement(54588..55349)
                     /gene="kduD"
                     /locus_tag="OO179_000893"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5531054.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55379..56215)
                     /gene="kduI"
                     /locus_tag="OO179_000894"
     CDS             complement(55379..56215)
                     /gene="kduI"
                     /locus_tag="OO179_000894"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5531055.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56502..57683)
                     /locus_tag="OO179_000895"
     CDS             complement(56502..57683)
                     /locus_tag="OO179_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5531056.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57938..59167
                     /locus_tag="OO179_000896"
                     /pseudo
     CDS             57938..59167
                     /locus_tag="OO179_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14748 a        16025 c        14540 g        14422 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc
    19801 tgatctccca taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt
    19861 tgccccatcg gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg
    19921 gaaagtcgcc gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac
    19981 cagaatggag ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt
    20041 tttatgcagc ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct
    20101 gtcagcaata gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc
    20161 gcttttgctc acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc
    20221 cgcagtactg gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg
    20281 actggtaaag gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt
    20341 tgctacgcgc acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat
    20401 attgccctct tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt
    20461 ttcgcgcgtt ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag
    20521 cataatgaca atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg
    20581 cggcacctgc ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc
    20641 ctgcatatcc tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc
    20701 gaccggcgca agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg
    20761 tacggtttgc gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg
    20821 aatttgtgcc gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac
    20881 cacgcgttca gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac
    20941 cgtcacgcgg gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc
    21001 agccaggctg acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg
    21061 acgacggctg attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag
    21121 ctgtgtaaca agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt
    21181 catctaataa acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct
    21241 ctcgcataaa atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca
    21301 aataatcatg caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga
    21361 ttccgccatt cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc
    21421 ggcggtgaag ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg
    21481 cacagcctcg gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat
    21541 ctggctgcgc atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa
    21601 acactggaac tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg
    21661 tcaatgagtc tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat
    21721 tttattattg cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt
    21781 atccggcgga ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta
    21841 atggggccgg tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt
    21901 gccactcaac tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag
    21961 ggcgttacta atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca
    22021 cgggtagaag cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt
    22081 ggcgcagtga aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa
    22141 gatggcgcgc tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg
    22201 gaaagcgccg agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg
    22261 attcgcccgc tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg
    22321 gaaatcgaca aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc
    22381 tatccgttcc cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac
    22441 cgcagttcat cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc
    22501 ggcttaagca aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga
    22561 tttaccccgg tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag
    22621 cgtaaatcca aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc
    22681 aggcgtaaca ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg
    22741 ccgccagacc actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat
    22801 cggcatacag cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg
    22861 ttactaccgg cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct
    22921 gcgatttatg taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca
    22981 cagacttaat attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca
    23041 gcgcaatacc gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca
    23101 ccggtcggcc ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca
    23161 taaactgttc aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc
    23221 acaaaagctg gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca
    23281 gcagatccag ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt
    23341 cgccgctctg taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca
    23401 ctgccgtttt atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc
    23461 cgaaacgata gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc
    23521 cggttcctgc cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg
    23581 taaagcccgc gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg
    23641 ccagttgatc acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca
    23701 gtctcgacat cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat
    23761 gcagcgggtt accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc
    23821 ggtgcaaagt gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact
    23881 gtcgcaaagc cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag
    23941 ccagacggat acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg
    24001 ccacggtggt cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca
    24061 gcgccactgc cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt
    24121 ccagggtttg cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga
    24181 agcgcgctac cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga
    24241 ggatcatcgg cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt
    24301 cccaattttg tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct
    24361 cgttattttc cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta
    24421 acagtgccgc cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt
    24481 gcacatccag cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt
    24541 tcattacgcc tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc
    24601 ggcgttgggt cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag
    24661 gaacagatag ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg
    24721 cagataacga tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg
    24781 catcgctgct gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga
    24841 tttatagtcg agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt
    24901 taacatgccg cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa
    24961 ctggcgaatt agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa
    25021 ctccatctcc acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt
    25081 gagaggtgcc tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc
    25141 aaagccgccg agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc
    25201 gaggtcttca aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg
    25261 cggtgttaac gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg
    25321 tggcatcaga tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac
    25381 acgccagtta tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc
    25441 atcattaacc tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc
    25501 atcgcataac gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg
    25561 cagcaaacgc ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg
    25621 gcgcaccagc ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta
    25681 aagcaaacgc agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc
    25741 agcattaaga tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg
    25801 gacgcggaaa ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt
    25861 atggatcgtg acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga
    25921 ggcattactg tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact
    25981 ttccagctgc gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg
    26041 ccgctcaccg cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc
    26101 ccgcagcatc ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc
    26161 gactaccgca tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag
    26221 ccccatcatt gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac
    26281 cgcctgcaac aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt
    26341 cgaaaggtaa acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc
    26401 ctggcggctg cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc
    26461 gttcatcagc aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc
    26521 acatacctgc gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag
    26581 ccacattttc atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt
    26641 cccggctgat ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg
    26701 gctgaaaagc ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa
    26761 agtgtagtgg gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg
    26821 gaatgcatat atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg
    26881 gtgccagata cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat
    26941 cattgccacc gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg
    27001 cagcgcggaa tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt
    27061 ttcacgcgct actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat
    27121 cgacaatggt tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc
    27181 ccccccggcc ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg
    27241 caactgataa ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc
    27301 ctgattgcta cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag
    27361 ttcacccact gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc
    27421 gtggcgggaa gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc
    27481 ttgcagataa cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac
    27541 gacctgggct atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc
    27601 ctggtagcgt agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc
    27661 aaaggcattc aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc
    27721 atccatctgc cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc
    27781 ttccagcagg cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa
    27841 ctcgtggata ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt
    27901 gaccaccagc agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc
    27961 aagtaacagg cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc
    28021 ttcaatcagg cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc
    28081 actcattctt ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca
    28141 ctttccagcc ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg
    28201 acagaatagc catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt
    28261 gcggcgtcag cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc
    28321 cgcgatcgaa atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca
    28381 gcatctgggt aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca
    28441 attttgcctc tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt
    28501 tatcattgct ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg
    28561 caaacacggc atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta
    28621 cgatctgccc caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta
    28681 caaataccgc tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt
    28741 gttttttatc gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact
    28801 gtttttgcac atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca
    28861 taaactctgg tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg
    28921 taatggaggg caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca
    28981 tctgcgcggg cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct
    29041 ggttatacca ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc
    29101 cctcgagcaa tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca
    29161 taaggccgtt gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta
    29221 actgatcaag cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg
    29281 cgctgtccat ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg
    29341 caaaataacg gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg
    29401 gatggtcgta tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa
    29461 gctctggcaa agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg
    29521 cgctgatttt atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct
    29581 ctttcgggct gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct
    29641 ttactgcttt agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag
    29701 gaacgcgaat catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg
    29761 gataacggaa gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc
    29821 ctttttcacg taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag
    29881 ccaagccttt atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga
    29941 taggatcgga gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa
    30001 gtgtacctgg gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga
    30061 acttcgctga gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag
    30121 ggacgtaatg aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg
    30181 gttttttgct ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact
    30241 ccggcagcgg tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct
    30301 cgtggaaatc tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga
    30361 ggttaccacc agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct
    30421 gtgccatgcg catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac
    30481 gctcacgttc ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt
    30541 ctaccgcacc gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg
    30601 cagtgctggc attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct
    30661 gcgggtactt tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt
    30721 acgcctcggg atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg
    30781 cctgcggatc agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact
    30841 ggcggttatc tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg
    30901 cctgacttaa tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc
    30961 ggggcattca cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc
    31021 aaatcatctg ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat
    31081 cagtccgtga ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac
    31141 actcatgact gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg
    31201 gcctccattg tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct
    31261 tcgccacgca ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc
    31321 aacgtggaat cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac
    31381 gcgccgccac tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca
    31441 atcagttgtg agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca
    31501 ccgtctttgc gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg
    31561 tgttccagcc aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac
    31621 aggccatccg gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga
    31681 tcaatttcca tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc
    31741 atctcctggc actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct
    31801 gccgcccgga agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat
    31861 aactgctgat tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg
    31921 atttcgctgt cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc
    31981 ggatgtgccc agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc
    32041 agcggctgaa caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca
    32101 cgagcatagc tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta
    32161 tggaggcaag taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca
    32221 tcgcccggta gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac
    32281 gggaaacgtt cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc
    32341 tgcgcggaaa ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg
    32401 ccacgcttcg gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa
    32461 acgccgtcgt tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc
    32521 agagtacaaa tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga
    32581 cgctgtgcca gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct
    32641 aaaccttcgg cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt
    32701 tccgcatccg gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct
    32761 agcggacgct cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc
    32821 agatgcccca ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc
    32881 cactcgccct gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc
    32941 caggtgtgtt gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc
    33001 caacgaatgc cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg
    33061 tcaaaccgcg ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag
    33121 cgactgtcag gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc
    33181 gcacgacggt cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact
    33241 gcctgaataa acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga
    33301 gtaagtgtcg gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca
    33361 cgctgcgggc tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt
    33421 ttgttatcgc tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt
    33481 tccagaatgt cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg
    33541 tccagctcct ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc
    33601 ccccatgagg ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt
    33661 ccggcatttt cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc
    33721 tgacgagtca gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg
    33781 caggggttgg taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg
    33841 agataaacag gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc
    33901 gggcaggtcg tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc
    33961 cagcgcggtt gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc
    34021 caggcctgtg cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag
    34081 tccggacgat agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac
    34141 agttttcgct tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc
    34201 tccagcaatt gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg
    34261 ctctctttgg ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc
    34321 agcggaaaat caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac
    34381 tgcgccatac cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt
    34441 tcgcgttcga caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag
    34501 acccttaaca tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg
    34561 ccttctctgc ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat
    34621 gtctgcattc ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc
    34681 tgccaaccat gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat
    34741 gttcgctgga tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc
    34801 gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg
    34861 ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat
    34921 caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt
    34981 cattatctgc cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact
    35041 gaactgctgg ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga
    35101 tggcctggcg gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct
    35161 gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca
    35221 gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc
    35281 caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga
    35341 gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt
    35401 agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac
    35461 atgctccttc agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat
    35521 accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag
    35581 cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt
    35641 aaatacccgc agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc
    35701 acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat
    35761 agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct
    35821 ccttgctgat aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt
    35881 gagtttttga agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc
    35941 gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa
    36001 ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc
    36061 ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc
    36121 aaatagtccc gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc
    36181 cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca
    36241 atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga
    36301 cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg
    36361 ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac
    36421 actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc
    36481 ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg
    36541 ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga
    36601 gtttgatcca tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc
    36661 acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg
    36721 aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg
    36781 tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg
    36841 cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc
    36901 agtaccgtag taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt
    36961 ttaccataca ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca
    37021 ttgttttcgt gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg
    37081 atgatggaac gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta
    37141 aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt
    37201 gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa
    37261 acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc
    37321 atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc
    37381 gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac
    37441 aagcctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac
    37501 agcaccacac cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg
    37561 taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta
    37621 cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca
    37681 ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata
    37741 aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg
    37801 ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac
    37861 agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg
    37921 aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca
    37981 ctgcccggac gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga
    38041 tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac
    38101 tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga
    38161 aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat
    38221 agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc
    38281 tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga
    38341 tgacgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg
    38401 gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac
    38461 agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca
    38521 ccgcacaaac gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct
    38581 cgtaacattg caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca
    38641 acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt
    38701 gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt
    38761 tttggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg
    38821 cgtgcttcgt caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc
    38881 gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg
    38941 agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa
    39001 ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac
    39061 atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc
    39121 agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt
    39181 tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt
    39241 aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta
    39301 attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga
    39361 tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc
    39421 acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct
    39481 cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt
    39541 tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca
    39601 agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag
    39661 ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac
    39721 tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa
    39781 agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag
    39841 cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg
    39901 cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg
    39961 gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc
    40021 gtctggcgat attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc
    40081 tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt
    40141 tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac
    40201 gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca
    40261 tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac
    40321 gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa
    40381 caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag
    40441 atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg
    40501 actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa
    40561 gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac
    40621 tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac
    40681 cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt
    40741 tctgcatcgc cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc
    40801 ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt
    40861 gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac
    40921 atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc
    40981 tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg
    41041 tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg
    41101 aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta
    41161 ataacaattg aataactcac agttatgtgc agagttataa acagaggaag gggtggatag
    41221 ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga
    41281 aaacccggag cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca
    41341 atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct
    41401 catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa
    41461 gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa
    41521 cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc
    41581 tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc
    41641 ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac
    41701 tgcgaatatt cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc
    41761 aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc
    41821 aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga
    41881 atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg
    41941 cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg
    42001 tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta
    42061 atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga
    42121 taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc
    42181 tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg
    42241 atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga
    42301 taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac
    42361 ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg
    42421 cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt
    42481 accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa
    42541 cgcttggctt gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat
    42601 tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg
    42661 tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt
    42721 tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt
    42781 gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc
    42841 cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt
    42901 gcagattatg ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct
    42961 gtcagatcac ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt
    43021 cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct
    43081 ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc
    43141 gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat
    43201 tcgcaacaaa aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca
    43261 ctccttttac cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat
    43321 aaacagacta ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta
    43381 ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg
    43441 gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc
    43501 gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt
    43561 tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct
    43621 ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg
    43681 aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc
    43741 ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact
    43801 atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc
    43861 gccccgaaac gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca
    43921 gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg
    43981 gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc
    44041 tcaagcgcct acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga
    44101 ccaactgctt cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc
    44161 tggatacgct ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg
    44221 tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc
    44281 tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag
    44341 gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt
    44401 tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc
    44461 tctttagtcg gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg
    44521 ttgatatcgc cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct
    44581 gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta
    44641 acatcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac
    44701 atcaggttta tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat
    44761 attaatgacg gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga
    44821 agccaatgcc aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca
    44881 gcatcaacaa catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc
    44941 cgacgctttt tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc
    45001 ccagcacgcc aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga
    45061 aaatcggtac caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca
    45121 gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt
    45181 tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca
    45241 acaggaaacg cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac
    45301 gcgtttcacc attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga
    45361 tgagattcca ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga
    45421 ccgcaccacc gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca
    45481 gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac
    45541 cgttagcttt tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg
    45601 gtgaataggc tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa
    45661 agcagatact ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt
    45721 tggcgaagct atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa
    45781 acaccatttg caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca
    45841 gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg
    45901 ctttcatccc cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt
    45961 tccgcttcgt ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat
    46021 ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg
    46081 cgggcatact gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt
    46141 gcctcgcctt tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca
    46201 ccaagtgcca gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag
    46261 cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca
    46321 gtgtcatacc agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt
    46381 acacctggct tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg
    46441 cgtccgccct cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata
    46501 cgccctaccg taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat
    46561 tcggtcacac cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg
    46621 gtactttctt gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac
    46681 gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa
    46741 ctgcggtcat acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac
    46801 acttctgcac ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac
    46861 agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc
    46921 tcgacgttcg ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa
    46981 ccggaggtaa agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc
    47041 ggcatcagca aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa
    47101 tcttccagat agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct
    47161 ttatcgagaa actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg
    47221 gtcagccctt ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg
    47281 gccccaaaaa tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg
    47341 ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg
    47401 cgataggagt ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg
    47461 tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct
    47521 tccatcatta tttgatgcag catttcgcca gcaattttgc gacggtcacg ggcacgcggc
    47581 gcatccggca tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag
    47641 cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc
    47701 acaggaataa ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc
    47761 atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt
    47821 tccaccagac gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag
    47881 tcgataaatg atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca
    47941 aacactggac gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga
    48001 ttaggcgtaa ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg
    48061 cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct
    48121 ctgattcagt aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta
    48181 aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga
    48241 agagcgtacc gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg
    48301 acgactcttc gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa
    48361 attgtcggtt actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg
    48421 ctggtgtaat tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca
    48481 ccactttagc aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa
    48541 gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt
    48601 cccacgatga aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa
    48661 tgtaagcgtt tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg
    48721 caggcgtttc agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag
    48781 cttcccggct ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg
    48841 cccgtgcgct ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg
    48901 atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt
    48961 tcttattgat tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac
    49021 tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt
    49081 tagcctcatt aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct
    49141 ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt
    49201 taattcagca aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg
    49261 ccgaagatcg tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg
    49321 atcggctggt gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc
    49381 ttttgggacg aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg
    49441 gcgcgatggg cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg
    49501 gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc
    49561 caatcgtaac gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc
    49621 ctctcccgga aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa
    49681 ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc
    49741 agcgccagta attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca
    49801 aacagaactt ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat
    49861 gcccctgtat catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt
    49921 tcaacatttc ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg
    49981 acgacggttt ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg
    50041 atatggtggt aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc
    50101 agcacaaagt ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca
    50161 gactgcgcta ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg
    50221 cgagcgattt gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc
    50281 tcttcaccct gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc
    50341 tgaccgaatt cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa
    50401 tcaacgccag aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc
    50461 acgtccagtg cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg
    50521 gtattggttt tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga
    50581 tgccctggcg aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga
    50641 atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca
    50701 atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct
    50761 aacgagacgg aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg
    50821 gaacaggctt tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga
    50881 cgaataattt gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc
    50941 agattttcgg cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc
    51001 agatgagtgc ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat
    51061 gtcgagtcta tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc
    51121 gttaacttac gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag
    51181 gcagcacacc tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag
    51241 aaggtgatcg ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa
    51301 ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc
    51361 gccgcagaaa gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc
    51421 ttttcgcaat cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc
    51481 agcttaaata tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt
    51541 catgatttag gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta
    51601 ctctctttag atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta
    51661 ttaacgccgg atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat
    51721 cgccagttgc tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa
    51781 acccacagcg ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt
    51841 aacccgctca ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt
    51901 gcggttccgt tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt
    51961 caggcgttta gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct
    52021 attttgtcgt cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg
    52081 tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc
    52141 caaaaatgac gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag
    52201 cgcgtgacgc ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt
    52261 tcgcccgttc atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc
    52321 cgcgataaaa atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc
    52381 cggccccggt aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac
    52441 gtgactcaat ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg
    52501 cgcccgcccg ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt
    52561 ccccgcgacg ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg
    52621 cagagtgaag cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa
    52681 tggtggattc ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat
    52741 gtgtgttacc gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc
    52801 caatctctat taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac
    52861 gccgatattt ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac
    52921 atttttggtt tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt
    52981 gtagagccag aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc
    53041 gataatcata ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca
    53101 tttcagcggc tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata
    53161 accggcaata cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt
    53221 accgttatca aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct
    53281 gaaaccaatt ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa
    53341 ggtcgcgaac ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt
    53401 cgtaaagccc gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt
    53461 aaactgctgc atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt
    53521 gatcttaaac agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag
    53581 ttcttctcgc gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat
    53641 atgacgcccc ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat
    53701 cagcagaact gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact
    53761 gaacgctgta tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat
    53821 actgatcatc ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt
    53881 gtaagacgcg atcccgacag caatgcccag caccacacga gcggcgatta acatctctac
    53941 gctggtcgca aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag
    54001 gctgtattta cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc
    54061 gccgagcatc atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc
    54121 ggtaatgaac ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc
    54181 gaccgcagca gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg
    54241 cgttaaagca gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca
    54301 ttgtgaaaaa atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg
    54361 attaaattgc tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa
    54421 ttattgagat tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta
    54481 attgataatt gatatggatt atttcataac catgatatgg attatgatga tctagagata
    54541 taaaaaaccc tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc
    54601 caaccgccat ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga
    54661 aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg
    54721 agaatttccg cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg
    54781 taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat
    54841 cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc
    54901 tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag
    54961 tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg
    55021 tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc
    55081 accaggatat caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca
    55141 tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc
    55201 tcgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc
    55261 agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta
    55321 ccttggagag aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact
    55381 agcgcaaatc tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca
    55441 tgccccagat aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg
    55501 agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca
    55561 tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct
    55621 cgtgggtgtg acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca
    55681 tactcaattg acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt
    55741 tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg
    55801 tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat
    55861 caatactggc aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac
    55921 cgatttcata gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac
    55981 ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa
    56041 cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt
    56101 aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc
    56161 cttgggtatc cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa
    56221 cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc
    56281 tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt
    56341 tatttttaat ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg
    56401 gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat
    56461 taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg
    56521 gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta
    56581 cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg
    56641 tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc
    56701 ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca
    56761 gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc
    56821 atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa
    56881 ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg
    56941 gatgatgcat tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct
    57001 aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga
    57061 ccgttacttt ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc
    57121 gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca
    57181 tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac
    57241 aacccatcat gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg
    57301 ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata
    57361 tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg
    57421 caaacgtcat taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat
    57481 tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc
    57541 accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt
    57601 tccacggcgg aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc
    57661 ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg
    57721 attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata
    57781 tgcctgagca gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca
    57841 atagatgggt agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc
    57901 tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga
    57961 agaaactctg tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct
    58021 tcctattcag ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg
    58081 gcctttaaca tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc
    58141 agcaggtgaa gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct
    58201 gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat
    58261 taccaactca cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat
    58321 gttggtgagt ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc
    58381 cactattcgg gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc
    58441 catttacctt gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca
    58501 gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac
    58561 gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga
    58621 taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt
    58681 tgtctttagc tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg
    58741 ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc
    58801 cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt
    58861 tattgaaggt gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag
    58921 tcgtgcattt aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg
    58981 ttgcattaac ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat
    59041 gatacttttc atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg
    59101 ttccatcgga aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt
    59161 tagctaatat ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc
    59221 ataatattca tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc
    59281 agttagttaa acacacaaag agatctgaac atcttaaata agactattta agatgcataa
    59341 cttagattcg caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca
    59401 taccatgaga tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata
    59461 atcatattta ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac
    59521 agattaatcg caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat
    59581 caccaatgaa attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag
    59641 tcgttataaa agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga
    59701 gtcaccgcgt ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAJTNW010000014        58575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNW010000014 DAJTNW010000000
VERSION     DAJTNW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..1100)
                     /locus_tag="OO179_000897"
     CDS             complement(<1..1100)
                     /locus_tag="OO179_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5531057.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO179_000898"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO179_000898"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5531058.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO179_000899"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO179_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5531059.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO179_000900"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO179_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5531060.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO179_000901"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO179_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5531061.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO179_000902"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO179_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5531062.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO179_000903"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO179_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5531063.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO179_000904"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO179_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5531064.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO179_000905"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO179_000905"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5531065.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO179_000906"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO179_000906"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5531066.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO179_000907"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO179_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5531067.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO179_000908"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO179_000908"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5531068.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO179_000909"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO179_000909"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5531069.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO179_000910"
     CDS             11850..12014
                     /locus_tag="OO179_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531070.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO179_000911"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO179_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5531071.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO179_000912"
     CDS             13523..13768
                     /locus_tag="OO179_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5531072.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO179_000913"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO179_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5531073.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OO179_000914"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OO179_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5531074.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OO179_000915"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OO179_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5531075.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OO179_000916"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OO179_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5531076.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OO179_000917"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OO179_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5531077.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OO179_000918"
     CDS             19431..19784
                     /locus_tag="OO179_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5531078.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OO179_000919"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OO179_000919"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5531079.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OO179_000920"
     CDS             21084..22211
                     /locus_tag="OO179_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5531080.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OO179_000921"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OO179_000921"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5531081.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24393)
                     /locus_tag="OO179_000922"
                     /pseudo
     CDS             complement(24291..24393)
                     /locus_tag="OO179_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24447..26421
                     /gene="pdeR"
                     /locus_tag="OO179_000923"
                     /pseudo
     CDS             24447..26421
                     /gene="pdeR"
                     /locus_tag="OO179_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26569..26742
                     /locus_tag="OO179_000924"
     CDS             26569..26742
                     /locus_tag="OO179_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531082.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26832..27581
                     /locus_tag="OO179_000925"
     CDS             26832..27581
                     /locus_tag="OO179_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5531083.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27850..28068
                     /gene="osmB"
                     /locus_tag="OO179_000926"
     CDS             27850..28068
                     /gene="osmB"
                     /locus_tag="OO179_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5531084.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28194..28520)
                     /gene="yciH"
                     /locus_tag="OO179_000927"
     CDS             complement(28194..28520)
                     /gene="yciH"
                     /locus_tag="OO179_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5531085.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28520..29257)
                     /gene="pyrF"
                     /locus_tag="OO179_000928"
     CDS             complement(28520..29257)
                     /gene="pyrF"
                     /locus_tag="OO179_000928"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5531086.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29450..30619)
                     /gene="lapB"
                     /locus_tag="OO179_000929"
     CDS             complement(29450..30619)
                     /gene="lapB"
                     /locus_tag="OO179_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5531087.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30626..30934)
                     /gene="lapA"
                     /locus_tag="OO179_000930"
     CDS             complement(30626..30934)
                     /gene="lapA"
                     /locus_tag="OO179_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5531088.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31083..31847)
                     /gene="pgpB"
                     /locus_tag="OO179_000931"
     CDS             complement(31083..31847)
                     /gene="pgpB"
                     /locus_tag="OO179_000931"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5531089.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32017..32607
                     /gene="ribA"
                     /locus_tag="OO179_000932"
     CDS             32017..32607
                     /gene="ribA"
                     /locus_tag="OO179_000932"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5531090.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32671..35346)
                     /gene="acnA"
                     /locus_tag="OO179_000933"
     CDS             complement(32671..35346)
                     /gene="acnA"
                     /locus_tag="OO179_000933"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5531091.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35510..35605)
                     /locus_tag="OO179_000934"
     CDS             complement(35510..35605)
                     /locus_tag="OO179_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531092.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35719..35886)
                     /locus_tag="OO179_000935"
     CDS             complement(35719..35886)
                     /locus_tag="OO179_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531093.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35889..36053)
                     /locus_tag="OO179_000936"
     CDS             complement(35889..36053)
                     /locus_tag="OO179_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5531094.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36348..37322)
                     /gene="cysB"
                     /locus_tag="OO179_000937"
     CDS             complement(36348..37322)
                     /gene="cysB"
                     /locus_tag="OO179_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5531095.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37532..40129)
                     /gene="topA"
                     /locus_tag="OO179_000938"
     CDS             complement(37532..40129)
                     /gene="topA"
                     /locus_tag="OO179_000938"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5531096.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40509..40760
                     /locus_tag="OO179_000939"
     CDS             40509..40760
                     /locus_tag="OO179_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5531097.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40796..41845)
                     /gene="sohB"
                     /locus_tag="OO179_000940"
     CDS             complement(40796..41845)
                     /gene="sohB"
                     /locus_tag="OO179_000940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5531098.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42065..42823
                     /locus_tag="OO179_000941"
     CDS             42065..42823
                     /locus_tag="OO179_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5531099.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42820..43410
                     /gene="cobO"
                     /locus_tag="OO179_000942"
     CDS             42820..43410
                     /gene="cobO"
                     /locus_tag="OO179_000942"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5531100.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43450..44322)
                     /gene="rluB"
                     /locus_tag="OO179_000943"
     CDS             complement(43450..44322)
                     /gene="rluB"
                     /locus_tag="OO179_000943"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5531101.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44423..45043)
                     /locus_tag="OO179_000944"
     CDS             complement(44423..45043)
                     /locus_tag="OO179_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5531102.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45040..45921)
                     /gene="yciV"
                     /locus_tag="OO179_000945"
     CDS             complement(45040..45921)
                     /gene="yciV"
                     /locus_tag="OO179_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5531103.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46059..46103
                     /gene="trpL"
                     /locus_tag="OO179_000946"
     CDS             46059..46103
                     /gene="trpL"
                     /locus_tag="OO179_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5531104.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46080..46173
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46195..47757
                     /gene="trpE"
                     /locus_tag="OO179_000947"
     CDS             46195..47757
                     /gene="trpE"
                     /locus_tag="OO179_000947"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5531105.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47757..49352
                     /gene="trpD"
                     /locus_tag="OO179_000948"
     CDS             47757..49352
                     /gene="trpD"
                     /locus_tag="OO179_000948"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5531106.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49353..50714
                     /gene="trpCF"
                     /locus_tag="OO179_000949"
     CDS             49353..50714
                     /gene="trpCF"
                     /locus_tag="OO179_000949"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5531107.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50726..51919
                     /gene="trpB"
                     /locus_tag="OO179_000950"
     CDS             50726..51919
                     /gene="trpB"
                     /locus_tag="OO179_000950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5531108.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51919..52725
                     /gene="trpA"
                     /locus_tag="OO179_000951"
     CDS             51919..52725
                     /gene="trpA"
                     /locus_tag="OO179_000951"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5531109.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="OO179_000952"
     CDS             53106..53285
                     /locus_tag="OO179_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5531110.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="OO179_000953"
     CDS             53371..53871
                     /locus_tag="OO179_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5531111.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OO179_000954"
     CDS             53917..54423
                     /locus_tag="OO179_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5531112.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO179_000955"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO179_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5531113.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OO179_000956"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OO179_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OO179_000957"
     CDS             55478..56221
                     /locus_tag="OO179_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5531114.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OO179_000958"
     CDS             56251..56790
                     /locus_tag="OO179_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5531115.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OO179_000959"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OO179_000959"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5531116.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO179_000960"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO179_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5531117.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="OO179_000961"
     CDS             58276..58572
                     /locus_tag="OO179_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5531118.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14062 a        15149 c        14646 g        14718 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtagaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacaa accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcacctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg cctgaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaaa agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggc aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca cactctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccact
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaacaat
    24361 gcgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact
    24421 ccgctgttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca
    24481 actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat gctttgcgct
    24541 tttctaccac caaaaccaca gaacctgatc gtacattgca gttatctgcc gaacaggctg
    24601 ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg
    24661 aaagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca
    24721 acgcgtctgc atggcatgac acaccggcgg ttgctcgtga tttatcacac gggctttcct
    24781 ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga
    24841 atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaaagaa cacgacgtca
    24901 ttgggtaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca
    24961 ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac
    25021 gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa
    25081 acgagatttt tttaatctgt tccggtaccg acattaccga agagcgccgc actcaggagc
    25141 gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc
    25201 aggagttaat cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc
    25261 ttgatttgga taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt
    25321 tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc
    25381 gtccaggtgg cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag
    25441 caatggcatc acgaattttg acccgcttac gcctcccctt tcgtattggt ttaattgaag
    25501 tttataccag ctgttcagta ggtatttcgc tctctcccga acatggttcg gacagcgcga
    25561 ctattattcg tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat
    25621 tttgcgtttt tactccagaa atgaatcagc gggtatttga atatctctgg ctggatacca
    25681 acttgcgtaa agcactggaa aacgatcagt tggttatcga aaatcaccta gcgtggcgaa
    25741 gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt gattccaccg
    25801 ttggacttca tttcctacgc cgaagagtca gggctaattg tgcctttagg ccgttgggtg
    25861 attctcgatg tcgtacgcca ggtggcaaag tggcgggata aaggcataaa cctgcgagtg
    25921 gcggtaaata tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag
    25981 gttctccagg aactcaattt tgaatactgc cctatagatg ttgaactgac agagagttgt
    26041 ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagcca actaggtgcg
    26101 caagtgcatc tggatgattt tggtaccggc tactcttcac tttcgcaact ggcgcgcttt
    26161 ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag atattcacaa acaacctgtc
    26221 tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg cattgaatct tcaggtgatc
    26281 gccgaaggtg tagagagtgc aaaggaagat gcttttttaa ccaagaacgg gatcaatgag
    26341 cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa
    26401 cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata tcccgtcatt
    26461 atatatcgta ttcctggatg ataattagcc gttgctgtta aacacttctc ttttagcggc
    26521 aatttttgca ttattcattt tccccctttt gctataagga tgtccttgat gtctgaattt
    26581 gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat
    26641 taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct
    26701 gaatctacgc cagacatccc gaagacaccg tgggagattt aacaatcgtg ccttcaggtt
    26761 ttcctatttg aggatgcccg gcgcgtcagt atttttttat ttagtattat aacgttataa
    26821 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
    26881 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
    26941 atctcaacac cctcgaaaaa ctgagttacc tccgccgtgc acatggcttt gcagtttcgc
    27001 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
    27061 ccgagtttgc cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggcagca
    27121 gcaatgccct gctcgcccgg actctggggg agcagaagaa aaatgtcact atcatcacgg
    27181 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg
    27241 gcgtgtacca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccagc
    27301 aggtacattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actggattca
    27361 ccggtcgcga tatgatgcgc accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag
    27421 cgatagtcct gactgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg
    27481 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
    27541 ttgagcacag taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg
    27601 atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact
    27661 atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat aacaggaagt
    27721 aattatcacc tgcgtgatat aaccctgcgc gcgagcagat ttcacggaat aatttcacca
    27781 ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga
    27841 gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattactttg
    27901 gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggc
    27961 gcgggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta
    28021 ggtggtgcag ctgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat
    28081 gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat
    28141 aatacacaca gtaaaatatt cgaaagccac ggatatatcc gtggcttttt atgttaaccg
    28201 cctgcgagtt ttaccttcat ccctttcgct tcaagtagtg attttaataa atcacgctta
    28261 tcgccctgga tttcaataac tccatcttta actgctccgc cgcagccgca ttttttcttc
    28321 agttccgctg caagttttgt cagttcggca tcatcgagat cgataccggt aatcaggcaa
    28381 acgcccttac ctttacgtcc actggtctga cgctgaatac gcaccgctcc atcgccttta
    28441 gggcgtactg gggccgcttt gggctcatca atacgcccgg tttccgttga gtagaccaga
    28501 cggctgttgg aatcactcat catgcactcc gctgtaaaga ggcgttgatc gctttcagtg
    28561 tctgcgctgg atctaccgat tgcgttaccg ggcgaccaat caccatataa tcaacaccag
    28621 ccgccaacgc ctgttctggc gtcataatgc ggcgctggtc accagcttca ctcccctgcg
    28681 gacgaatgcc cggtgtaacc agtttgaact cctgaccgaa tacctgttta aagcgcacag
    28741 cttcctgagc agaacacacc acaccatcaa ggtcacattt ttgcgtcagt gctgccagac
    28801 gttctgcata atctgcaggt gacagtgtca cgccaagatc ggccaggtcg ctggcttcca
    28861 tgctggtcaa cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagtg
    28921 cctcacgcgc tgcggtcatc atacgcgccc caccggaggc atgaacattc accatccaca
    28981 cgcctaagtc agctgcagca gcgacagcgt gcgctgcagt gttggggata tcgtggaatt
    29041 tcaggtcaag aaagatatca aaaccacgct gttgaagttc gcgcacaaac tgtggcccaa
    29101 acaatgtaaa catctcttta ccgaccttca gacgacaatc gcgtgggtcg atcttgtcga
    29161 caaaggacag cgcgtcatca caattatgat aatcaagggc aacaaccaca ggagaattcg
    29221 taacggcgcg ggaagaagat gaagcagtta acgtcatgac cagaccttct tgatgatggg
    29281 caccgggagt ggcgcgaaac aggtaaacgg cgagcattct acccgtgaaa ataaaaaatt
    29341 aacagttgcg atttttcccc tagcaggctt tttctgctcc gtcgttgccg gagcacaatg
    29401 ggacatcaat aactagcatg ttgtaactaa agtcggattt ttttaaaaat tacaggccat
    29461 caagaccgcg aatcggttta atagttgacc aggcacgaca agacggacaa tgccagtaga
    29521 gggtgtatgc agtaaaacca catttctggc agctataacg aggcttacta cgcaccttct
    29581 cgccaaccat gtcacgcagc accatcagac tctctttggc gcgcccttct tccgcttcat
    29641 ttaagtggta atccattagc ttatggaata cacgcatggt cggatgacgc tgaagctggc
    29701 gtgtaatata gacctgtgca gcctcactac cgtcgcgcgc ttcgatgata tcagcaagca
    29761 tcaattcagc atcggcaccg gtgttctctt ccaccgcgcg ttgcaggaat tctgcccatt
    29821 cggcagtttt acccaactgc tgatagcagg tttgcaacat ttccagcgtt tcgctgacca
    29881 gttctctgtc ctgggatatg acgcgttgca aactttcgac ggctttggcg tattctcctt
    29941 tcgccataaa cacgcgtccc atcatgatgg atacgcgggc gctgttttta tctgccgccg
    30001 cccctttttt tagcaaggtc atggcacgat cgagatcgtc gctggccata tgctgcaggg
    30061 ctaactcaca gtagaaatgg gcaatttcga cgcgctgttt atctttaccc agcttcacca
    30121 ggcgttcggc aacatcaatt gctttctgcc actcactggt agcctggtag atttgtagca
    30181 actgttgcag cgcgccaatg cggaagtcag tttcatcggt cagctgattg aacatgtctt
    30241 ccgcgcggtc gtataacccg gcggccatgt aatcacgccc cagttgttga atcgccaaca
    30301 gacgctgttc ataggtcagc gaggcgcttt ccattagggt ctgatggatg cgaatagcgc
    30361 gatcaacttc gccacgcgaa cggaacaggt ttccgagcgt aaggtgggct tcaacggtgc
    30421 ctgtatcctc tttaagcata tcgagaaaca ggtctaccgc tttatcctgt tgattactaa
    30481 gcaggaagtt aacccccgct acgtaatcac gcgacaagcg gttggcttca tcttgcttgt
    30541 tttgttgcgc acttctgcgg cccatatacc agccataggc agcggctaca ggcaaaagca
    30601 gaaacaacaa ctccagcata gaaagttatt ccttcgccgc tgacgagtgc ggcactacag
    30661 ccacgtcagt cgcgggtgaa agctggtttt ccagtcgctt tattttacgt tcagcgcgcg
    30721 ccagggaaac acgaactcgc agccagaaca ggccacaaat caaccaaccg atagcaaacc
    30781 ccgcagcaaa caataccgcc agcaatgtgg aaatacggta ctccccttgc gctaacagat
    30841 aattaaacgt cacctgttga tcgttctgcg cacccaacgt gaccgaaatc acgaagatcg
    30901 ctaacaccag taagaaaatg agtaaatatt tcacattact tcccgttatg tggttagagc
    30961 gaataaatta tggtcaactt accgcaatag ccctttaaag taccattttc gcgccgtctg
    31021 cgaaatggca tccacgcgca ttgttgatga tttaaggcct aaattcggaa aatcaaccag
    31081 cgttaacttt cttgttctcg ttgtgcaatt tcgcgatttt cttccgcagg tggtgttaat
    31141 ggcccacaaa ttcgttgcgc aagccacgtt gccaccgcca ccagcgccca cgaaatcaac
    31201 gtagctacca ccagatcgcg tggccaatgc atcccgagca gcaggcggct tcccatgact
    31261 cccgttgccc agaccaacaa gatagcaatg gttaacgttc gccgacgcgg ccacagcaaa
    31321 ccaacggcca gcagtgccca actggcagca aacatcgtgt gaccggaagg aaaggcaaac
    31381 cccgtctctt tctgccagtg tgaacgcaaa tattgtggaa tatttttctc ttcagccaac
    31441 tgttctttta ctagatttcc gcgttctgct cgctttaaag tgtagaactc atcaaccgga
    31501 atatgatgtg ttttttccaa ccagataaca aaaggtcgtg gttcctgtac tttgtctttg
    31561 atccaggatt taacgccttg tccaacaagg attgccgccg ccagaatggc aaataataca
    31621 aaggcagcct taatgcgaaa acgcagacac cagagaaacc agccgaacaa aatcaaatgt
    31681 gtaatgacgc cccagggctg ggtgacagtt tcagtaaccc aaaaagccgc ttttagtagc
    31741 caactttgtt ctccaggttg ccaacgccag ccagaaatcc atacggctac tggcatgaca
    31801 agcaatagtg cagctcccac tgcggtacgt cgggcaatcg aacgcatggc ctctcctttt
    31861 tgataagtcc cacaatcata actgaaaatg ccagttccaa gaaaaattga cagatttgtg
    31921 ccattccgtg aacgatcgac gcgtcgtgat taggtgaacc ccttctcgtt atggcaaaat
    31981 aagccaatac agaaccagca ttatctggag aatttcatgc agcttaaacg tgtggcagaa
    32041 gccaaactgc caaccccatg gggcgatttc ctgatggtgg gatttgaaga actggcaacc
    32101 ggacacgatc atgtcgcgct agtctatggc gatatttccg ggcatacccc ggtacttgcg
    32161 cgcgtccatt ccgaatgtct gaccggtgac gccctgttca gcttgcgctg cgattgtggc
    32221 ttccagctcg aagcggcatt gacgcaaatt gccgaggaag gccgtggtat tttgctgtat
    32281 caccgtcagg aaggtcgtaa cattggtctg ctgaataaaa tccgcgctta cgcactgcag
    32341 gatcaaggtt acgataccgt agaggctaac caccagttag gcttcgccgc tgatgagcgc
    32401 gacttcactc tttgcgctga tatgttcaaa ctccttggcg tcaatgaagt ccgcttgtta
    32461 accaataacc cgaaaaaagt cgaaattctg accgaagcag ggattaatat tgttgaacgc
    32521 gtaccattga ttgtaggtcg taaccccaat aacgaacatt atctcgatac caaagccgag
    32581 aaaatgggcc atttgctgaa caaataaccc tcttgcatcg tgtaattcat ttgcttgccg
    32641 gaagcaaaat aaccggcaag caaatacttg ttacttcaac atattacgaa tgacataatg
    32701 caaaatgccg tcgttctggt agtaggtcaa ctccgtcgcg gtgtcgatac gacaacggca
    32761 gggtacgact tcctggctac catccgcgcg cgtaagcgtc accggaaccg tcgcgccagg
    32821 ttgtagattt tgcagatcgc caatatcaat cttctcttcc ccggttagcc ctaacgtttt
    32881 acgcgtcacg ccttgcggaa attccagcgg caggatgccc atgccaatta aattcgaacg
    32941 gtgaattcgt tcaaacgatt cggcaatcac cacacgaatt ccaagcagac gaggtccttt
    33001 tgccgcccag tcacggcttg agcctgatcc atactctttc ccggcaatca ccgccagcgg
    33061 cgtttgctcc tgcttatagc gcatcgcagc atcataaata gagactacgt cgctgtcagg
    33121 taaatgccgc gtcatccccc cttcaacgcc aggcaccatt tcattacgga tgcgaatatt
    33181 ggcgaaggtg ccgcgcatca tcacttcatg gttaccacgc cgcgaaccgt aggagttaaa
    33241 gtcttttcgc tcaacacccc gaccttgtag atatcgaccc gctgggctgt cgggcttaat
    33301 actgcccgcc ggagagatat ggtcagtggt gactgaatcc cccagcattg cgaggatccg
    33361 cgcaccgtga atatcttcca ctggtgctgg tgttgcctgc atttcatcaa agaaaggcga
    33421 taagcgaata taggttgagt cctcctgcca accgtaggta tcggatcgtg tgacgttaat
    33481 ttccttccac tctgctgtgc cttcaaaaac ttctgcgtac tctttgcgga acatttctgt
    33541 ggagacttgt tctaccgcac gggcaatttc ttgtgccgat ggccagatat ctttcagata
    33601 aaccggctcg cctttgcgat catggccgat aggctcagaa gccaggttga tattcatatt
    33661 tcccgccagc gcataggcaa ccaccagcgg tggcgaggcc agccagttag ttttaaccag
    33721 cggatggata cggccttcaa agttacggtt gccggacagc accgcaccga cggttaaatc
    33781 gccttttttg attgccgttt cgataggatc gggcagcggc ccagagttac caatacaggt
    33841 ggtacaaccg tatcccacaa ggttaaaccc cagttcgtcg agatacggtg tcagtttcgc
    33901 ttttgccaga taatcagaaa cgactttcga acccggtgcc aacgacgctt tgacccacgg
    33961 ttgccgcttg aggccaagag ttacggcttt tttcgccagc aagcctgcgg ccatcagcac
    34021 acttgggtta gaggtgttgg tgcacgaggt tatcgcagca atgaccacag cgccatcagg
    34081 taactgatac tgatgtccgt tcataacata atcgactggc aggcgatctt tatgcgtggc
    34141 attcacttcc agttcgttac tggcggcaaa tgcttttggt acatcgggca gtgcaacgcg
    34201 atcctgtggg cgttttggcc ctgccaggct cgcttcaacg tcattcatat ccagttctaa
    34261 catactggta aaaatcggtt catcgcccgg gttacgccac atgccctgcg ctttggcata
    34321 tttttcgacc aactcgacct gatcttcgct gcgcccgctt aaacgcatgt aatcgagggt
    34381 tactgcatcg attgggaaga agccacaggt ggcaccatat tctggcgaca tattggcaat
    34441 ggtggcgcga tccgccaacg gtagtgaatc cagaccatca ccataaaatt cgacgaattt
    34501 ccccaccacg ccatgtttgc gcagcatttg ggtaacagtg agaaccaggt ctgtggcggt
    34561 aataccttca cgtaattttc cggtaagttt aaagcccact acgtccggga taagcatgga
    34621 aaccggctgg cctaacatgg cggcttctgc ttcgatccca cccacgcccc accccagcac
    34681 gccaaggccg ttgatcatag tggtgtgcga gtcagtacca acgagtgtat ccggataagc
    34741 aatccattca ccgtcctgca attcgctcca cactgctttg ccgagatatt cgaggttaac
    34801 ctgatggcaa atgcctgtgc ctggcggcac gacgctaaac cgactgaacg cttgctttcc
    34861 ccatttcagg aacacataac gttcgtggtt gcgctccatt tccaggcgta cgttttcttc
    34921 aaatgcctca tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac
    34981 cggtgagagc gggttaacct ttgcagtatc gccaccgagg cgtttaaccg cttcgcgcat
    35041 tgccgccaga tcaacaacgg caggtacgcc ggtaaagtcc tgcatcagca cccttgccgg
    35101 gcggtaggca atttcacggt cagcatgggc atttttcagc catcccgcca gtgcgtggat
    35161 atcctcttcg gtaactgagt taccatcctg ccagcgcagc aggttttcga gcaaaacttt
    35221 gagtgacttg ggtagacggg tgatatcgcc cagtgattta gcagcaagcg gcaggctgta
    35281 gtagtggtaa gttttatctt tggcctgcaa cgtgtcctta ctggcttctc gtagggttga
    35341 cgacatagct cctccttaat gacagggttg cgaagccctg attctcttca gggtgaatat
    35401 taaagataac acaaacatcg cgtaacgatt tgataacaac ccaaattgat aaaagaggag
    35461 aaaccttgtg gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa
    35521 tgatccagag gagttcggcc cagaagagca tcgacagaga aaacgcgccc atccaggacc
    35581 aatatttcat atttgtgttg atcataggct acacccttaa cggttattat tttgtgttga
    35641 gataactcaa gctgttattg ctgcggtgta ttaaaagccc tttaattcat taaaaatggg
    35701 taataagaat gatgataatt attgtttcgg ctgatcatct tcagcaaaca gttctataaa
    35761 ggcttgctgc tgcgatgtta gttgccagtc gtctggaata acgacggaac attcccgttt
    35821 cgtttctggt tcattgtcac tatgctggca taatggtgac gactccagtt gctcctgacc
    35881 gaccatagtt atccccacac tttccaaatc agcagtgcga caaccaccca gaaaagtgca
    35941 gaaccaagaa aaaccgccag ccaggcttta cgttttaatt caggatcacg gcggggttcc
    36001 tgatttccag aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg
    36061 aatgattggt gtaattaatc atacgatgag acaaatccta aagaaacttt agtcaaaaat
    36121 ccagcgaatt tacgaatcgt ctccagtgaa taattcaatc ttttgacctg aaatagatat
    36181 ttgaaaactg gaatttgcga ggcgggtaat tagacactac tttacaggta atttacccta
    36241 taaatatgag ggataaaaat attgtgcacg gtatagaaat aagtaacaaa agagaatata
    36301 ttccggcacc ttcgctacat aaaaggtgcc gaaaataacg caagaaatta tttttccggc
    36361 agttttatat ctttaaacat gacctcaatt tcttcattgg agcgcaatgc gacagccgca
    36421 tcaacgacat cacgcgttaa atgcggtgca aaacgctgaa tgaaatcata catataactg
    36481 cgcaaaaaag tactacggcg aaaaccaatt ttggttgtac tgtggctgaa gatatcgtga
    36541 gcatcaacgc gcacaaggtc gggatcggcg accggatcca ccgccatgct ggcaatgacc
    36601 cctaccccca gccctaaccg gacgtaagtt ttaatgacgt cagcatccgt tgccgtgaaa
    36661 acgatacgcg gcgttaaccc tgcgcggtta aaggcagtat ccagttctga acgtccggta
    36721 aagccgaagg tatatgtcac caacggatat tgcgccagtt cttcaatggt aatggctttt
    36781 ttgcctgcca gcgggtgatc cggagtgact acaatagccc gattccagtg gtagcacggt
    36841 aacatcacta aatcttcata cagatgcagc gcttctgtgg cgatagcgaa atcggcattg
    36901 cctttagaga cggcatcagc aatttgtgtc ggcgggccct ggtgcatatg caaagaaacg
    36961 cggggataac gctcaataaa gcctttgata acgtttggta acgcgtagcg tgcctgggta
    37021 tgcgtggtgg cgatatacag cgagccttta tccggccagg tgtgctcacc ggcaaccgat
    37081 tttatggcat cgactttcga caggacttcg cgagcgatac gaattatttc ttgtcctgct
    37141 ggcgttacct gcgtcaggtg cttgccgctt cgggaaaaaa tttgaatgcc tagctcgtct
    37201 tccagcattc tgacttgttt actgatcccg ggttgtgatg tgtaaagtcc ttccgctgtt
    37261 gatgagacat tcaggttatg attgaccacc tcaacaatat agcgaagttg ttgtaatttc
    37321 atgttaaacc atccacttag acgccatcag aacatcgttt tttcgtcaca cctggcgggt
    37381 gactaatacg atttaataat aaaaaaggtg ctaactataa ccactatatc atttatagcc
    37441 ttagtgtata ggtgtgaaga aaaaataata tgcaataaaa aagggccgct ttcgcgaccc
    37501 tttgtttata aaaacctgac agaattaaag gttatttttt tccttcaacc catttgccat
    37561 caacataaaa tgctgaccag ccagtcgcct ttccgtcttt ttccgaagag acatattgct
    37621 gtttggtttt acggctaaag cgaaccatgg tcttattacc ttccggatcc tgctgtggcg
    37681 catcggccag ataacgcagt ttttccggca gacggtcgcg gaagcgataa agctcttcca
    37741 ccagtggcgc acgcgtttca cgcgatttcg ggaaagtgtt ggcagccagg aacacaccgg
    37801 cagcaccgtc acgcagcacg aaataagcat ctgatttttc gcacggcagc tcaggtaatg
    37861 gcaccggatc ttctttcggt ggtgccactt cgccgttacg taaaatctta cgtgtgtttt
    37921 tacactcttc gttggtgcag gccatgtatt taccgaaacg ccccattttc aggtgcattt
    37981 cagagccaca tttttcacac tcaacgatcg ggccgtcata acctttaatg cggaattcgc
    38041 cctcttcgat ctcgtaaccg tcgcaggttg ggttattacc acagacatgc aacttacgtt
    38101 tcggatcgat gagatagctg tccatcgccg tgccgcattt cgggcaacga cgttttgcgc
    38161 gcagcgcgtt ggtttcagca tcttcgcctt ccagcacgtt cagcacttcg ttttccggca
    38221 ccaggttaat ggtggttttg caacgctctt tcggcggcag cgcatagcca gaacagccaa
    38281 ggaatacccc ggtactcgct gtgcgaatcc ccattttgcg accacaagtc gggcagtcaa
    38341 tgctggtcag aaccatctgg ttcgggcgca taccaccctc ttccggatct ttttcagctt
    38401 tatctaactg ctgggtgaaa tccgagaaga agtgatccag tacagctttc cactctgctt
    38461 cgtgatttgc cacctggtcg aggctgtttt ccatctgcgc ggtaaagtcg tagttcatta
    38521 actcgcggaa attttcttca aggcgatcgg tgacgatttc gcccattttt tccgcataga
    38581 aacgacgatt ttctactcgc acatagccac gatcctgaat ggtcgaaatg atcgacgcat
    38641 aggtagacgg acgaccgata ccacgttttt ccagttcttt aaccagcgat gcttcactga
    38701 aacgggctgg cggcttggta aagtgctggg ctggtgttag ttcaacgagc gtcagagcat
    38761 cgcctttatc gactgccggt aagatacgat cttcatcgcc tttacgcagt gcaggcatca
    38821 cttttgtcca gccatcaaag cacaaaatac gaccgcgtgc tttcaggcgg aaatcgcccg
    38881 caccaacggt cagcgtcgtg gagtcatatt tcgctggggt catctggcag gcaacgaact
    38941 gacgccagat taactggtac agtttctgcg catctgcttc catatccttc agcgattccg
    39001 ccatcacatt gacgtcagaa ggacgaatcg cttcgtgcgc ttcctgtgag ttttctttgc
    39061 tggcgtactg attcggactt tccggcagat atttcttacc aaaattatcg ctgatataac
    39121 cgcgaaccat atttaccgcg tcctgactca ggttagtgga gtcggtacgc atgtaagtga
    39181 tatagcctgc ttcatacaaa cgctgcgcca tcatcatggt ttttttcacg ccaaaaccaa
    39241 gacgggtgct ggcagcttgt tgcagcgtag aggtaataaa aggagcgcca ggtttactgg
    39301 ttgtcggttt gtcttcacgt tccagcacgc tgtagcgcgc tttttccagc agacttaccg
    39361 cagcctgagt ttgttctttg ttgaccggac ggaacggttt gtcattctga tgagtcacct
    39421 gcaacgccaa cgcttcacca gatggcgtag tcgtgctggc atcgacttcc cagaactctt
    39481 ccggtacgaa cgctttaatt tcacgctcac gctctaccac caggcggacc gccaccgact
    39541 gcacacgacc ggcagacaga ccacgagcga tctttttcca cagcagcggc gaaaccatat
    39601 accccaccac gcggtccata aagcgacgcg cctgctgggc attaacacga tcaatattca
    39661 gctcacccgg tttgttaaat gcctggcgga tcgcgttttt agtaatttcg ttaaacacca
    39721 ctcggctata gcgcgcatca tcaccaccaa tcacttcccg caggtgccat gcaatggctt
    39781 ccccttcgcg gtcaaggtcg gttgcgagat agatgtggtc ggctttttca gccagttgtt
    39841 tcagttcaga gacgaccttc tctttaccag gcaacacttc atagtgcgcc tcccaattgt
    39901 gccacgggtc aacccccata cggttgacga gagcgccacg ttcatccttt ttaggctttt
    39961 tagccgtctt ggtggaggta gagtcggcac tctttttggc agctgagcca ctggtcggca
    40021 aatcgcggat gtgaccgacg ctggatttca ccacgtagtc acaacccaga tacttgttga
    40081 tcgttttggc ttttgccggg gactcaacga tgacaagagc tttacccata ttcaccttac
    40141 ctaatttgat tcttccagga atgcgtcgca cgttgataaa ccttccactg gcaacgagtt
    40201 accgatatgg actcgctctc tgcggatatc aaccccttgt cgtgactctg ttcacgaaaa
    40261 ttcaggtgac tgagttaaca ggtcttggcc tacaggcgct atagcacgac ggaattttgg
    40321 tcgaatgtca agcaaatcta ttgccagatt gacgaaagcg tcacactgta cctgataaaa
    40381 tccgatacgc aactttatta gcatgcaaaa tccaaagttg ccagtaaggc gctcttgcca
    40441 ccagcgtggt atatttgtct gcaagccatg cctggcgtag actaatgtcc ttgtttaagg
    40501 agtaaataat gaacaaagag acacaaccca tcgaccgcga aacgctgttg aaagaggcca
    40561 acaaaatcat tcgtgagcat gaggatacgc tcgcagggat tgaagccacc ggcgtcacgc
    40621 agcgtaatgg cgtgctggta tttaccggcg actacttttt agatgaacaa ggattgccaa
    40681 cagccaaaag caccgcggtg tttaatatgt tcaaacacct ggcccatgtg ctttcagaaa
    40741 aatatcatct ggttgattaa agcaaaacgc gaggtcttag cctcgcgttt gtcttttaca
    40801 tcaatggctt ttgaccccgc tgccaccagc gtagcaacaa tcgatcggcg ctctctgccg
    40861 cgctgccggt gaatcggtca atgagtcgtt tacgctgcat atagcgtaca ttaaccactt
    40921 cacggccttc catcaggcta agaataactt catcactggt gttgatttca tcaaccaggc
    40981 ctttctctac cgcctgttgt ccgtaccagt gttcacccgt tgccacctgt tcaatatcca
    41041 gagacggacg catacgcttc acaaaatctt taaataactg atgcgtttcg ttcagctctt
    41101 cgcggaattt ctcccgccct tcttcggtat tttcacccag caaggtcagc gtgcgcttat
    41161 actgcccggc ggtgtgcagt tcgatatcaa tatctttgct tttcaggaag cggttaaagt
    41221 tgggcatttg cgccaccacc ccaatggaac ccacaatagc aaacggtgcg gaaacaattt
    41281 tgtccgccac acaggccatc atgtaaccgc cgctggcagc gactttgtct accgtaacag
    41341 ttaaaggaat gtttttatca cgcagacgct gcagctgcga agccgccaac ccgtaaccat
    41401 gcaccatgcc accagggctt tccagacgga gcacaacctg atcctgcggt ttgaatgctg
    41461 cgagtaccgc cgttatctct tcacgtagcg agttcacttc atgggcgtcc atgctgcctt
    41521 taaaatccag cacccagacg cgaggtttac tgtcagttgc cacctcgccc agtttggctt
    41581 tcgcttttgc tgctttcgct tcttgcttgt gctttttctt ctgcgctttg tgccactgtt
    41641 tttgctgatg tgagtccatc agcgccgcgg ccagttcttc tttcatctcc ttatactgtt
    41701 cgctgagatt gttgacccgt aactcgccac gctggcgttt attacgttga gcaacattga
    41761 caataatggc ggcaatcgcc gcaatcgcta gcacaacggt aacgatttta gccaaaaaca
    41821 aaccatattc agacaacaat tccacgagtc cccaccttgg ttaaacaacg ctttggctca
    41881 cagtttacaa caggcatgtt aagccgtctc gcagagatgt tattgcagcg aaagtatgac
    41941 aaagtatcca ttttgcggtg ttatcgcccc aggctgattg aaatgccctt ctgtttcagg
    42001 cataaagccc caaagtcata aagtacactg gcagcgcgac agacaacgtc gttgaggagt
    42061 agccatgcat taccagccaa aacaagattt actcaatgat cgcattatcc tggtgacggg
    42121 agccagcgat gggattggtc gtgaagccgc gatgacgtat gcacgctatg gtgcgacagt
    42181 gattctgttg ggccgtaatg aagaaaaatt acgtcaggta gccagccaca taaacgaaga
    42241 aactgggcgt cagccacagt ggtttattct cgatttgctg acctgcacgt ccgaaaattg
    42301 ccaacaactg gcacagcgca ttgtcgttaa ttatccgcgt ctggatggtg ttttgcataa
    42361 tgccggattg ctcggcgatg tttgcccaat gagcgaacaa aatccgcagg tctggcagga
    42421 cgtcatgcag gtcaacgtta atgccacctt tatgctcacc caggcactgc ttcctttatt
    42481 actcaaatcg gacgccggtt cactggtctt tacttcatca agcgttggac gtcagggacg
    42541 agccaactgg ggtgcatatg cagcgtcgaa atttgccacc gaagggatga tgcaggtact
    42601 ggccgatgaa tatcagcagc gcctgcgcgt caactgcatt aacccaggcg gtacgcgcac
    42661 cgcaatgcgc gcaagcgcct tcccgaccga agatccacag aaacttaaaa cacccgctga
    42721 tatcatgccg ctctacctct ggctgatggg cgatgacagc cgccgtaaaa ccggcatgac
    42781 ctttgacgcc caaccgggcc gtaaaccagg aatttcccaa tgagtgatga acgctaccaa
    42841 cagcgtcagc agcgagtgaa agaaaaagta gatgctcgtg tggcccaggc ccaggatgaa
    42901 cgcggtatta tcatcgtctt taccggcaat ggaaaaggca aaaccaccgc ggcatttggt
    42961 acggcaacac gcgcagttgg tcacggaaaa aaagtgggcg tcgtgcagtt tattaaaggc
    43021 acctggccta atggcgaacg caatctgctg gagccacatg gcgttgagtt tcaggtgatg
    43081 gcaacgggct ttacctggga tacacaaaac cgcgagtctg ataccgccgc ctgccgcgaa
    43141 gtctggcaac atgcaaagcg gatgcttgct gattcctcac tggatatggt tttgcttgat
    43201 gaactgacgt atatggtggc gtatgactat ttgccactgg aagaagtggt gcaggcgtta
    43261 aatgaacgtc cacatcaaca gacggtgatt atcacgggtc gaggctgtca tcgggatatt
    43321 cttgaactgg cggatacggt aagtgagctg cgccccatca gacatgcgtt tgatgccggt
    43381 gttaaggcgc aaatagggat tgattattaa tctgtaaaaa tgccggatat gacgtccggc
    43441 atactttgat taaccgttat tacgcccgcc agaacgacga ctgccgctca cctgactgtg
    43501 acgtttcacc gcacgacgaa tctgattagc cttcatgcga cgacggtctt tttctacagc
    43561 gactttagag ctggtttctg gcggaagctc caccagttcg cgcagatagt tagtctgggc
    43621 gaggtccagc tccgtccagc caccgcgtgg cagacctttt ggcagtggga tatcaccgta
    43681 acgaacacgg atcaggcggc tcacctgcac accaaccgct tcccacagac gacgaacttc
    43741 acggttacgg ccttcggtca gagtcacgtt gtaccactgg ttgatccctt cgccgccgct
    43801 gaacttgatg gttttaaaag ctgccggacc atcttccagt tgcacgccac gactcaaatc
    43861 acgcagtttc gcgtcgtcaa cctgaccaaa tacacgcacg gcgtattcac gttcaacttc
    43921 acggcttgga tgcattaaac ggtttgccag ttcaccatcg gtggtgaaca gcagcagacc
    43981 acaggtgtta acgtccagac gacctacggc aatccagcgt gcgccacgca gctttggcag
    44041 acggtcaaac accgtcggac gcccttccgg atcgttacgg gtacacaact caccttccgg
    44101 tttgtaatag gccagaacgc gacaaatttg ttcagcggac tcacgtaccg aaatcagatg
    44161 accatcgata cgaattttca gcccaggggt aacttcaacg cgatcgccga gtttagcaat
    44221 tttgccatca acactcacac gaccggcttc aataatagat tcgatttcac ggcgggagcc
    44281 gtggccagcg cgggccagca ctttctgtaa tttttcgctc atagagcttc cttttggtgt
    44341 cgccttcaca ggcgtcgaac aggagaatca atggcgccgt atagcgccat tttaaggccg
    44401 cgtagtatac agagttacgc acttataaga aaggcttcac atcacctacg ccttcacgca
    44461 ccacgaccgg cgtatcatcg gtaagatcaa taaccgttgt tggtttctgc ccgagatagc
    44521 cgccgtgaat aatcaaatct acctgttttt ccagacggtc tttaatttct tcgggatcgg
    44581 attcggtaaa ttcgctgcct ggcagcatta gcgaagtgga aagcatcggt tcgcccagtg
    44641 cttcaagcaa cgcctgcgca atcgggttag aaggcacacg cataccgatg gttttgcgtt
    44701 tctcctgcaa cagacggcgt ggcacttcct tcgtcccctt caggatgaag gtatagttgc
    44761 ccggcgtgtt gtttttcatc aaacgaaacg caacgttatc cacgaatgaa taggtcgaga
    44821 gttcagaaag atcgcgacac atcagggtaa agttgtgacc gtccggcagc tggcgaatac
    44881 gacaaatacg ctccatggcg tttttatctt caattttaca gccgagcgca tagccggaat
    44941 cagttggata gacaatcacc ccgcctttac gcacgatctc caccgcctgg ttgatcagac
    45001 gttgctgcgg gttatcagga tgaatataaa aaaactggct cataattccc tctctgtggt
    45061 gttctgcggc tgttcccata gctgccagac gccttcaacg cctgcgggca gccagagttt
    45121 acgacccagt tcgatccacg ggcatggctg atgaaaatca gatccttgtg atgcccataa
    45181 atgatgctga cgcgcaagtg tggccagctg ggtgcgttca ttgggcgatt gctggcactg
    45241 cgcgacttcc atcgcgtcgc cgtggtgttc ggcaaaatgc gcaaccaatc ttttcagcca
    45301 tttagcacta agattgtacc gcccaggatg agctagcacc gccttaccgc cagaatgatg
    45361 aatgacatca atagcttgtt ctattgtaca ccactgtggc ggaacgtatc cggttttccc
    45421 gcgcgccaga tactttttaa agacatctgc cattgaactg gctttgccgc actccacaag
    45481 gaaacgcgca aaatgaccgc gcgtcactgc gccgccctgc gccagtcgtt gtgcgccttc
    45541 cagcgcccca ggtatttgcg ctttttcaag tctttcggca atttgctgcg cccgctgatt
    45601 gcgccgttct gtctgctgcg cgaggaactc acacattagc ggatgagtaa tatcaatatt
    45661 cagcccgaca atatgaattt catgattttc ccagaccgtg gaaatttcca cgccgggaat
    45721 aagattcagc gccagtcccg aacgtgaaat ttcctctctt gctggcgcga ttgcagctgt
    45781 ggtgtcatgg tcggtgatcg ccagggtgcc gacgcgcatc tcgactgcac ggtgcaccaa
    45841 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
    45901 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
    45961 tcataacggg tctggcaaat attctgaaat gagctgttga caattaatca tcgaaccagt
    46021 taactagtgc gcaagttcac gtaaaaaggg tatcgacaat gaaagcaatt ttcgtactga
    46081 aaggttggtg gcgcacttcc tgaaacgggc agtgtattca ccatgcgtaa agcaatcaga
    46141 tacccagccc gcctaatgag cgggcttttt tttgaacaaa attagagaat aacaatgcaa
    46201 acacaaaaac cgactctcga actgctaacc tgcgaaggcg cttatcgcga caacccgact
    46261 gcgctttttc accagttgtg tgggaatcgt ccggcaacgc tgctgctgga atccgcagat
    46321 atcgacagca aagatgattt aaaaagcctg ctgctgatag acagtgcgct gcgcattaca
    46381 gcattaggtg acaccgtcac aattcaggca ctttccggca atggcgaagc cctgctggca
    46441 ctactggata acgccctgcc tgcgggtgtg gaaagtgaac aatcaccaaa ctgccgtgtg
    46501 ctgcgcttcc cccctgtcag tccactgctg gatgaagacg cccgcttatg ctccctttcg
    46561 gtttttgacg ctttccgttt attgcagaat ctgttgaatg taccgaagga agaacgagaa
    46621 gcaatgttct tcggcggcct gttctcttat gaccttgtgg cgggatttga agatttaccg
    46681 caactgtcag cggaaaataa ctgccctgat ttctgttttt atctcgctga aacgctgatg
    46741 gtgattgacc atcagaaaaa aagcacccgt attcaggcca gcctgtttgc tccgaatgaa
    46801 gaagaaaaac aacgtctcac tgctcgcctg aacgaactac gtcagcaact gaccgaagcc
    46861 gcgccgccgc tgccagtggt ttccgtgccg catatgcgtt gtgaatgtaa tcagagcgat
    46921 gaagagttcg gtggcgtagt gcgtttgttg caaaaagcga ttcgcgctgg agaaattttc
    46981 caggtggtgc catctcgccg tttctctctg ccctgcccgt caccgctggc ggcctattac
    47041 gtgctgaaaa agagtaatcc cagcccgtac atgtttttta tgcaggataa tgatttcacc
    47101 ctatttggcg cgtcgccgga aagctcgctc aagtatgatg ccaccagccg ccagattgag
    47161 atctacccga ttgccggaac acgcccacgc ggtcgtcgcg ccgatggttc actggacaga
    47221 gatctcgaca gccgtattga actggaaatg cgtactgacc ataaagagct gtctgaacat
    47281 ctgatgctgg ttgatctcgc ccgtaatgat ctggcacgca tttgcacccc tggcagccgc
    47341 tacatcgccg atctcaccaa agttgaccgt tattcctatg tgatgcacct cgtctctcgc
    47401 gtagtcggcg aactgcgtca cgatcttgac gccctgcacg cttatcgcgc ctgtatgaat
    47461 atggggacgt taagcggtgc gccgaaagta cgcgctatgc agttaattgc cgaggcggaa
    47521 ggtcgtcgcc gcggcagcta cggcggcgcg gtaggttatt tcaccgcgca tggcgatctc
    47581 gacacctgca ttgtgatccg ctcggcgctg gtggaaaacg gtatcgccac cgtgcaagcg
    47641 ggtgctggtg tagtccttga ttctgttccg cagtcggaag ccgacgaaac ccgtaacaaa
    47701 gcccgcgctg tactgcgcgc tattgccacc gcgcatcatg cacaggagac tttctgatgg
    47761 ctgacattct gctgctcgat aatatcgact cttttacgta caacctggca gatcagttgc
    47821 gcagcaatgg tcataacgtg gtgatttacc gcaaccatat tccggcgcag accttaattg
    47881 aacgcctggc gacgatgagc aatccggtac tgatgctttc tcctggcccc ggtgtgccga
    47941 gcgaagccgg ttgtatgccg gaactcctca cccgcttgcg tggcaagctg cccattattg
    48001 gcatttgcct cggacatcag gcaattgtcg aagcttacgg gggctatgtc ggtcaggcgg
    48061 gcgaaattct tcacggtaaa gcgtcgagca ttgaacatga cggtcaggcg atgtttgccg
    48121 gattaacaaa cccgctgccg gtggcgcgtt atcactcgct ggttggcagt aacattccgg
    48181 ccggtttaac catcaacgcc cattttaatg gcatggtgat ggcagtacgt cacgatgcgg
    48241 atcgcgtttg tggattccag ttccatccgg aatccattct caccacccag ggcgctcgcc
    48301 tgctggaaca aacgctggcc tgggcgcagc agaaactaga gccagccaac acgctgcaac
    48361 cgattctgga aaaactgtat caggcgcaga cgcttagcca acaagaaagc caccagctgt
    48421 tttcagcggt ggtgcgtggc gagctgaagc cggaacaact ggcggcggcg ctggtgagca
    48481 tgaaaattcg cggtgagcac ccgaacgaga tcgccggggc agcaaccgcg ctactggaaa
    48541 acgcagcgcc gttcccgcgc ccggattatc tgtttgctga tatcgtcggt actggcggtg
    48601 acggcagcaa cagtatcaat atttctaccg ccagtgcgtt tgtcgccgcg gcctgtgggc
    48661 tgaaagtggc gaaacacggt aaccgcagcg tctccagtaa atccggctcg tcggatctgc
    48721 tggcggcatt cggtattaat cttgatatga acgccaataa atcgcgccag gcgctggacg
    48781 agttaggcgt atgtttcctc tttgcgccga aatatcacac cggattccgc cacgcgatgc
    48841 cggttcgcca gcaactgaaa acccgcaccc tgttcaatgt gctggggcca ttgattaacc
    48901 cggcgcatcc gccgctggcg ttaattggtg tttatagtcc ggaactggtg ctgccgattg
    48961 ccgaaacctt gcgcgtgctg gggtatcaac gcgcggcggt ggtgcacagc ggcgggatgg
    49021 atgaagtttc attacacgcg ccgacaatcg ttgccgagct gcatgacggc aaaattaaga
    49081 gctatcaatt gaccgctgaa gattttggcc tgactcccta ccaccaggag caactggcag
    49141 gcggaacacc tgaagaaaac cgtgacattt taacacgctt gttacaaggt aaaggcgacg
    49201 ccgcccatga agcagccgtc gctgcgaacg tcgccatgtt aatgcgcctg catggccatg
    49261 aagatctgca agccaatgcg caaaccgttc ttgaggtact gcgcagtggt tccgcttacg
    49321 acagagttac cgcgctggcg gcacgagggt aaatgatgca aaccgtttta gcgaaaatcg
    49381 tcgcagacaa ggcgatttgg gtagaaaccc gcaaacagca gcaaccgctg gccagttttc
    49441 agaatgaggt tcagccgagc acacgacatt tttatgatgc gctacagggt gcacgcacgg
    49501 cgtttattct ggagtgtaaa aaagcgtcgc cgtcaaaagg cgtgatccgt gatgatttcg
    49561 atccggcacg cattgccgcc atttataaac attacgcttc ggcaatttcg gtgctgactg
    49621 atgagaaata ttttcagggg agctttgatt tcctcctcat cgtcagccaa atcgccccgc
    49681 agccgatttt atgtaaagac ttcattatcg acccttacca gatctatctg gcgcgctatt
    49741 accaggccga tgcctgctta ttaatgcttt cagtactgga tgacgaccaa tatcgccagc
    49801 ttgcagccgt cgctcacagt ctggagatgg gggtgctgac cgaagtcagt aatgaagagg
    49861 aactggagcg cgccattgca ttaggggcaa aggtcgttgg catcaacaac cgcgatctgc
    49921 gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc gccgaaactg gggcacaacg
    49981 taacggtaat cagcgaatcc ggcatcaata cttacgctca ggtgcgcgag ttaagccact
    50041 tcgctaacgg ttttctgatt ggttcggcgt tgatggccca tgacgatttg cacgccgccg
    50101 tgcgccgggt gttgctgggt gagaataaag tatgtggcct gacgcgtggg caagatgcta
    50161 aagcagctta tgacgcgggc gcgatttacg gtgggttgat ttttgttgcg acatcaccgc
    50221 gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc ggcaccgttg cagtatgttg
    50281 gcgtgttccg caatcacgat attgccgatg tggtggacaa agctaaggtg ttatcgctgg
    50341 cggcagtgca actgcatggt aatgaagatc agctgtatat cgatacgctg cgtgaagctc
    50401 tgccagcaca tgttgccatc tggaaagcat taagcgtcgg tgaaaccctg cccgcccgcg
    50461 agtttcagca cgttgataaa tatgttttag acaacggcca gggtggaagc gggcaacgtt
    50521 ttgactggtc actattaaat ggtcaatcgc ttggcaacgt tctgctggcg gggggcttag
    50581 gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc cggacttgat tttaattctg
    50641 ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt ggcctcggtt ttccagacgc
    50701 tgcgcgcata ttaaggaaag gaacaatgac aacattactt aacccctatt ttggtgagtt
    50761 tggcggcatg tacgtgccac aaatcctgat gcctgctctg cgccagctgg aagaagcttt
    50821 tgtcagcgcg caaaaagatc ctgaatttca agcccagttc aacgacctgc tgaaaaacta
    50881 tgccgggcgt ccaaccgcac tgaccaaatg ccagaacatt acagccggga cgaacaccac
    50941 gctgtatctc aagcgtgaag atttgctgca cggcggcgca cataaaacca accaggtgct
    51001 ggggcaggcg ttgctggcga agcggatggg taaaaccgaa atcatcgccg aaaccggggc
    51061 cggtcagcat ggcgtggcgt cggcccttgc cagcgccctg ctcggcctga aatgccgtat
    51121 ttatatgggt gccaaagacg ttgaacgcca gtcgcctaac gtttttcgta tgcgcttaat
    51181 gggtgcggaa gtgatcccgg tacatagcgg ttccgccacg ctgaaagatg cctgtaatga
    51241 ggcgctacgc gactggtccg gcagttatga aaccgcgcac tatatgctgg gcaccgcggc
    51301 tggcccgcat ccttatccga ccattgtccg tgagtttcag cggatgattg gcgaagaaac
    51361 caaagcgcag attctggaaa gagaaggtcg cctgccggat gccgttatcg cctgtgttgg
    51421 cggcggttcg aatgccatcg gcatgtttgc tgatttcatc aatgaaacca acgtcggcct
    51481 gattggtgtg gagcctggtg gtcacggtat cgaaactggc gagcacggcg caccgctaaa
    51541 acatggtcgc gtgggcattt atttcggtat gaaagcgccg atgatgcaaa ccgaagacgg
    51601 gcagattgaa gaatcttact ccatctccgc cggactggat ttcccgtctg tcggtccaca
    51661 acacgcgtat cttaacagca ctggacgcgc tgattacgtg tctattaccg atgatgaagc
    51721 ccttgaagcc ttcaaaacgc tgtgcctgca cgaagggatc atcccggcgc tggaatcctc
    51781 ccacgccctg gcccatgcgc tgaaaatgat gcgcgaaaac ccggataaag agcagctact
    51841 ggtggttaac ctttccggtc gcggcgataa agacatcttc accgttcacg atattttgaa
    51901 agcacgaggg gaaatctgat ggaacgctac gaatctctgt ttgcccagtt gaaggagcgc
    51961 aaagaaggcg cattcgttcc tttcgtcacg ctcggtgatc cgggcattga gcagtcattg
    52021 aaaattatcg atacgctaat tgaagccggt gctgacgcgc tggagttagg tatccccttc
    52081 tccgacccac tggcggatgg cccgaccatt caaaacgcca ctctgcgcgc ctttgcggca
    52141 ggtgtgactc cggcacaatg ttttgaaatg ctggcactga ttcgccagaa acacccgacc
    52201 attcccattg gcctgttgat gtatgccaat ctggtgttta acaaaggcat tgatgagttt
    52261 tatgcccagt gcgagaaagt cggcgtcgat tcggtgctgg ttgccgatgt gccagttgaa
    52321 gagtccgcgc ccttccgcca ggccgcgttg cgccataatg tcgcacctat cttcatctgc
    52381 ccgccaaatg ccgatgacga cctgctgcgc cagatagcct cttacggtcg tggttacaca
    52441 tatttgctgt cacgagcggg cgtgaccggc gcagaaaacc gcgccgcgtt acccctcaat
    52501 catctggttg cgaagctgaa agagtacaac gctgcacctc cattgcaggg gtttggtatt
    52561 tccgccccgg atcaggtaaa agcagcgatt gatgcaggag ctgcgggcgc gatttctggt
    52621 tcggccattg ttaaaatcat cgagcaacat attaatgagc cagagaaaat gctggcggca
    52681 ctgaaagttt ttgtacaacc gatgaaagcg gcgacgcgca gttaatccca cagccgccag
    52741 ttccgctggc ggcattttaa ctttctttaa tgaagccgga aaaatcctaa attcatttaa
    52801 tatttatctt tttaccgttt cgcttacccc ggtcgaacgt caacttacgt catttttccg
    52861 cccaacagta atataatcaa acaaattaat cccgcaacat aacaccagta aaatcaataa
    52921 ttttctctaa gtcacatatt cctcaggtaa ttgttaatat atccagaatg ttcctcaaaa
    52981 tatattttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agcgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcggtttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggtgacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtatggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttcggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gagta
//
LOCUS       DAJTNW010000015        58052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNW010000015 DAJTNW010000000
VERSION     DAJTNW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(27..281)
                     /locus_tag="OO179_000962"
     CDS             complement(27..281)
                     /locus_tag="OO179_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5531119.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            436..1008
                     /gene="yeiP"
                     /locus_tag="OO179_000963"
     CDS             436..1008
                     /gene="yeiP"
                     /locus_tag="OO179_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5531120.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1231..2697
                     /locus_tag="OO179_000964"
     CDS             1231..2697
                     /locus_tag="OO179_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5531121.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2815..3801
                     /gene="yeiR"
                     /locus_tag="OO179_000965"
     CDS             2815..3801
                     /gene="yeiR"
                     /locus_tag="OO179_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5531122.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3840..4553
                     /gene="lpxT"
                     /locus_tag="OO179_000966"
     CDS             3840..4553
                     /gene="lpxT"
                     /locus_tag="OO179_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5531123.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4965..5531
                     /gene="mepS"
                     /locus_tag="OO179_000967"
     CDS             4965..5531
                     /gene="mepS"
                     /locus_tag="OO179_000967"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5531124.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5712..7268
                     /locus_tag="OO179_000968"
     CDS             5712..7268
                     /locus_tag="OO179_000968"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5531125.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7350..9164
                     /locus_tag="OO179_000969"
     CDS             7350..9164
                     /locus_tag="OO179_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5531126.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9165..10259
                     /locus_tag="OO179_000970"
     CDS             9165..10259
                     /locus_tag="OO179_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5531127.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10259..11284
                     /locus_tag="OO179_000971"
     CDS             10259..11284
                     /locus_tag="OO179_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5531128.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11286..12875
                     /gene="yejF"
                     /locus_tag="OO179_000972"
     CDS             11286..12875
                     /gene="yejF"
                     /locus_tag="OO179_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5531129.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12879..13223)
                     /locus_tag="OO179_000973"
     CDS             complement(12879..13223)
                     /locus_tag="OO179_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531130.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OO179_000974"
     CDS             complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OO179_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5531131.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OO179_000975"
     CDS             complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OO179_000975"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5531132.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15619..17379
                     /locus_tag="OO179_000976"
     CDS             15619..17379
                     /locus_tag="OO179_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5531133.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17504..17788
                     /gene="rplY"
                     /locus_tag="OO179_000977"
     CDS             17504..17788
                     /gene="rplY"
                     /locus_tag="OO179_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5531134.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OO179_000978"
     CDS             complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OO179_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5531135.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19116..19343
                     /locus_tag="OO179_000979"
     CDS             19116..19343
                     /locus_tag="OO179_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5531136.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19363..21123
                     /gene="pbgA"
                     /locus_tag="OO179_000980"
     CDS             19363..21123
                     /gene="pbgA"
                     /locus_tag="OO179_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5531137.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21198..21274
                     /locus_tag="OO179_000981"
     tRNA            21198..21274
                     /locus_tag="OO179_000981"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21232..21234,aa:Pro,seq:ggg)
     gene            complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OO179_000982"
     CDS             complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OO179_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5531138.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24288..24935
                     /gene="narP"
                     /locus_tag="OO179_000983"
     CDS             24288..24935
                     /gene="narP"
                     /locus_tag="OO179_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5531139.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OO179_000984"
     CDS             complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OO179_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5531140.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OO179_000985"
     CDS             complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OO179_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5531141.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OO179_000986"
     CDS             complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OO179_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5531142.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OO179_000987"
     CDS             complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OO179_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5531143.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OO179_000988"
     CDS             complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OO179_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5531144.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OO179_000989"
     CDS             complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OO179_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5531145.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OO179_000990"
     CDS             complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OO179_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5531146.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="OO179_000991"
     CDS             complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="OO179_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5531147.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31445..32047)
                     /gene="napC"
                     /locus_tag="OO179_000992"
     CDS             complement(31445..32047)
                     /gene="napC"
                     /locus_tag="OO179_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5531148.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32057..32506)
                     /gene="napB"
                     /locus_tag="OO179_000993"
     CDS             complement(32057..32506)
                     /gene="napB"
                     /locus_tag="OO179_000993"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5531149.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32503..33366)
                     /gene="napH"
                     /locus_tag="OO179_000994"
     CDS             complement(32503..33366)
                     /gene="napH"
                     /locus_tag="OO179_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5531150.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33353..34048)
                     /gene="napG"
                     /locus_tag="OO179_000995"
     CDS             complement(33353..34048)
                     /gene="napG"
                     /locus_tag="OO179_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5531151.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34055..36541)
                     /gene="napA"
                     /locus_tag="OO179_000996"
     CDS             complement(34055..36541)
                     /gene="napA"
                     /locus_tag="OO179_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5531152.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36538..36801)
                     /gene="napD"
                     /locus_tag="OO179_000997"
     CDS             complement(36538..36801)
                     /gene="napD"
                     /locus_tag="OO179_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5531153.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36791..37285)
                     /gene="napF"
                     /locus_tag="OO179_000998"
     CDS             complement(36791..37285)
                     /gene="napF"
                     /locus_tag="OO179_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5531154.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37693..38181
                     /gene="eco"
                     /locus_tag="OO179_000999"
     CDS             37693..38181
                     /gene="eco"
                     /locus_tag="OO179_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5531155.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38331..39350)
                     /locus_tag="OO179_001000"
     CDS             complement(38331..39350)
                     /locus_tag="OO179_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5531156.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39584..39943)
                     /locus_tag="OO179_001001"
                     /pseudo
     CDS             complement(39584..39943)
                     /locus_tag="OO179_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40161..41804)
                     /gene="yojI"
                     /locus_tag="OO179_001002"
     CDS             complement(40161..41804)
                     /gene="yojI"
                     /locus_tag="OO179_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5531157.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41880..42530)
                     /gene="alkB"
                     /locus_tag="OO179_001003"
     CDS             complement(41880..42530)
                     /gene="alkB"
                     /locus_tag="OO179_001003"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5531158.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42530..43594)
                     /gene="ada"
                     /locus_tag="OO179_001004"
     CDS             complement(42530..43594)
                     /gene="ada"
                     /locus_tag="OO179_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5531159.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43668..44723)
                     /gene="apbE"
                     /locus_tag="OO179_001005"
     CDS             complement(43668..44723)
                     /gene="apbE"
                     /locus_tag="OO179_001005"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5531160.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44835..45956)
                     /gene="ompC"
                     /locus_tag="OO179_001006"
     CDS             complement(44835..45956)
                     /gene="ompC"
                     /locus_tag="OO179_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5531161.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46694..49366
                     /gene="rcsD"
                     /locus_tag="OO179_001007"
     CDS             46694..49366
                     /gene="rcsD"
                     /locus_tag="OO179_001007"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5531162.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49383..50033
                     /gene="rcsB"
                     /locus_tag="OO179_001008"
     CDS             49383..50033
                     /gene="rcsB"
                     /locus_tag="OO179_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5531163.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50233..53082)
                     /gene="rcsC"
                     /locus_tag="OO179_001009"
     CDS             complement(50233..53082)
                     /gene="rcsC"
                     /locus_tag="OO179_001009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5531164.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53357..54133)
                     /locus_tag="OO179_001010"
     CDS             complement(53357..54133)
                     /locus_tag="OO179_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5531165.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54138..55787)
                     /locus_tag="OO179_001011"
     CDS             complement(54138..55787)
                     /locus_tag="OO179_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5531166.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55788..56347)
                     /locus_tag="OO179_001012"
                     /pseudo
     CDS             complement(55788..56347)
                     /locus_tag="OO179_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56351..56509)
                     /locus_tag="OO179_001013"
     CDS             complement(56351..56509)
                     /locus_tag="OO179_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531167.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56506..>58052)
                     /locus_tag="OO179_001014"
     CDS             complement(56506..>58052)
                     /locus_tag="OO179_001014"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5531168.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15658 c        14266 g        13930 t
ORIGIN      
        1 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
       61 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
      121 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
      181 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
      241 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
      301 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
      361 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
      421 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
      481 aacggcaaac tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc
      541 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
      601 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
      661 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
      721 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
      781 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
      841 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
      901 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
      961 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
     1021 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
     1081 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
     1141 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
     1201 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
     1261 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
     1321 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
     1381 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
     1441 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
     1501 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
     1561 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
     1621 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
     1681 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
     1741 cgccgccgtg agcgcagcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
     1801 aatggccatg tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc
     1861 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
     1921 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
     1981 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
     2041 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
     2101 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
     2161 ttcgcctata tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta
     2221 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
     2281 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
     2341 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
     2401 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
     2461 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
     2521 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
     2581 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
     2641 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
     2701 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
     2761 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
     2821 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
     2881 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
     2941 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
     3001 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
     3061 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
     3121 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
     3181 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
     3241 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
     3301 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
     3361 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
     3421 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
     3481 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
     3541 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
     3601 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
     3661 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
     3721 gagctgattt ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt
     3781 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
     3841 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt
     3901 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
     3961 tctttaatca gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca
     4021 atcgcgcctt cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc
     4081 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
     4141 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
     4201 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
     4261 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
     4321 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
     4381 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
     4441 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
     4501 ccttttttga caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag
     4561 taattaacat catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc
     4621 tttcatctat cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
     4681 ctcaattgta ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat
     4741 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
     4801 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
     4861 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
     4921 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
     4981 cgattttgag atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg
     5041 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
     5101 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
     5161 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
     5221 gcagcactaa aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat
     5281 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
     5341 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
     5401 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
     5461 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
     5521 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
     5581 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
     5641 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
     5701 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
     5761 ttgtagcagg cgtgatgatt gcgatactgg gtagctgcct tcagttttta gtggcctggc
     5821 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
     5881 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
     5941 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
     6001 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
     6061 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
     6121 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
     6181 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
     6241 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
     6301 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
     6361 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
     6421 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc
     6481 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
     6541 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag
     6601 tactgctacg ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact
     6661 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
     6721 gcgatgccgc agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg
     6781 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
     6841 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
     6901 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
     6961 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
     7021 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
     7081 acgcggtgct gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa
     7141 cgccggaaca agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
     7201 ttagtcaccc gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc
     7261 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
     7321 aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg
     7381 ctctgttcac cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc
     7441 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
     7501 caaaaggtgg gcagataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg
     7561 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt
     7621 ccgatgacga accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg
     7681 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca
     7741 ttactgcccg cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat
     7801 ttcgtctggt ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg
     7861 agttagctaa acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag
     7921 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
     7981 gtccgtacca cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
     8041 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
     8101 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
     8161 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
     8221 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
     8281 cgtttaatat ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg
     8341 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
     8401 gttattttca aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
     8461 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
     8521 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
     8581 acgaagcggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
     8641 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
     8701 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
     8761 ctaaccgcat gcgcaatcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
     8821 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
     8881 cccccggcgt gcaaagcccg gttatcgact cactgatcaa ccaaattatt gccgcgcagg
     8941 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
     9001 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
     9061 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
     9121 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
     9181 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
     9241 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
     9301 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
     9361 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga
     9421 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
     9481 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
     9541 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
     9601 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
     9661 ttgacgtctg gagtagcaca tttatcatca tcggctacgc cattccggcc tttttgtttg
     9721 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
     9781 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
     9841 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
     9901 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
     9961 aaggggtaag tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg
    10021 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    10081 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    10141 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    10201 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    10261 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    10321 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    10381 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    10441 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    10501 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    10561 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    10621 ctggctggga acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg
    10681 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    10741 gggggcgcta caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga
    10801 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    10861 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    10921 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    10981 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    11041 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    11101 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    11161 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    11221 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    11281 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    11341 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    11401 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    11461 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    11521 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    11581 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    11641 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    11701 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    11761 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    11821 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    11881 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    11941 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    12001 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    12061 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    12121 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    12181 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    12241 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    12301 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    12361 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    12421 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    12481 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    12541 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    12601 cattaattct taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    12661 cggtacaggc gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct
    12721 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    12781 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc
    12841 aggagtatac gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    12901 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    12961 tggcgattta caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg
    13021 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    13081 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    13141 tcaaccttcg aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg
    13201 attgagagtt gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt
    13261 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    13321 cctcgcctgc gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    13381 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    13441 gactttttac ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt
    13501 gtggttgaca tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc
    13561 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    13621 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    13681 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    13741 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
    13801 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcggcgca ctgatgacca
    13861 tccacgcagc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    13921 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    13981 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc
    14041 cggcgcttaa gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca
    14101 ggacacgttt atggcggaac agcgccgcaa agttaccaat agtggtacga atgtaaaatg
    14161 gctgacgacg ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca
    14221 gaatcgccgc taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc
    14281 agccgccaac tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca
    14341 tcatccgcga gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc
    14401 tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
    14461 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg
    14521 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
    14581 gaatataagt actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa
    14641 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
    14701 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
    14761 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
    14821 cgggggctaa atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca
    14881 cgtggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
    14941 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt
    15001 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt
    15061 ccagtgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
    15121 actgaccatc atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg
    15181 cgtgcagttt ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt
    15241 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
    15301 gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg
    15361 cattacgaac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
    15421 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
    15481 tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccagt
    15541 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
    15601 atctacctac gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    15661 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    15721 gcaggtaaaa gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg
    15781 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    15841 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt
    15901 ggcagcgttc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    15961 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    16021 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    16081 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    16141 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    16201 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    16261 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    16321 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    16381 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    16441 gaagatgctg cactgattac tggcgacaac cccggcgctg agcgcgatgt gttaattgaa
    16501 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
    16561 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ctgaatcagt gagtctttac
    16621 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
    16681 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
    16741 agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    16801 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg
    16861 tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    16921 ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta
    16981 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
    17041 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    17101 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    17161 cagcgtaccg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    17221 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    17281 tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
    17341 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    17401 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    17461 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    17521 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    17581 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    17641 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctaacca tcgttgttga
    17701 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    17761 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    17821 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    17881 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag
    17941 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    18001 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    18061 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    18121 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta
    18181 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    18241 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    18301 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    18361 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    18421 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    18481 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    18541 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg
    18601 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    18661 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    18721 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    18781 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    18841 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    18901 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    18961 gtattcaacc accgccagtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
    19021 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    19081 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta
    19141 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    19201 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    19261 ttgccccggc ccaacgccag gcaattgcca actcttttgc cagcgcctta cagtcctcta
    19321 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    19381 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    19441 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    19501 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctagtgttc
    19561 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    19621 aggtttttgt ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa
    19681 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    19741 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    19801 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    19861 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    19921 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    19981 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    20041 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat
    20101 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    20161 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    20221 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    20281 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
    20341 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
    20401 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    20461 gtacgcaccc aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac
    20521 gcacaagaag ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac
    20581 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    20641 atcaaacgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt
    20701 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
    20761 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    20821 gctctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc
    20881 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    20941 tgggttactg ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    21001 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    21061 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    21121 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    21181 tattagcagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
    21241 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt
    21301 acggttggtt tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc
    21361 tgtttatttt ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt
    21421 ctacccctgc gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg
    21481 atgaccttat cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc
    21541 catcgtcatt aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca
    21601 gccagggttc cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg
    21661 cttcagcgcg taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt
    21721 agtcctgacc atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc
    21781 cgtcaaccca acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc
    21841 caaacgctgt tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt
    21901 tcaccacccc atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg
    21961 tatagctatc gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac
    22021 caaagaacaa gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga
    22081 gcgtcaggcc cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc
    22141 gggtgttaat tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa
    22201 gacgctcacg cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt
    22261 tcagcacatc agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat
    22321 ggttgccatt atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat
    22381 tgcccaacgt aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg
    22441 ctgccgggct ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg
    22501 tgacgttgag ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt
    22561 ttcccgacag ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg
    22621 aacgagtcac tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta
    22681 agttgaggcg agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat
    22741 tttcgagcga aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc
    22801 cctcagaaac cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca
    22861 tgacctcaag aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga
    22921 cattagcgcg gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga
    22981 tacttccatt ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac
    23041 cagtatcctt tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct
    23101 gcgtgtcctc cataacaatg agcccggaac catcatccag ttcataattt tttgacacac
    23161 catcctttat actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg
    23221 agacaattaa tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa
    23281 cctccagtaa gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc
    23341 gcaaacttcc gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact
    23401 gaccgagacg gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc
    23461 ctgctttctg atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct
    23521 tttcgatagc ggcaaaaacg gtccccccat ctttttcaac gattaagcgc ccgcctttaa
    23581 tcgtggtatc attcgctggg ccataaacct gtagcacacc attattgaga gtagtctggt
    23641 ttgccgcgcc acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg
    23701 taccattcat ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa
    23761 attcttttat atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa
    23821 ccagctggat atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg
    23881 atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac
    23941 aaagagaaac agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt
    24001 aaaagcattg ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact
    24061 gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc
    24121 tgatgcaaaa ttccgtcttt ataataaaaa tgatgccaaa gcgaacgaca aggttgtagt
    24181 tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa
    24241 cgctacgctc aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa
    24301 cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt
    24361 tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgaaggc gcgagcgcga
    24421 tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta
    24481 tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta
    24541 tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg
    24601 gttatctgtt gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga
    24661 aaggcagcaa agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg
    24721 gcgcggaaga agatcccttc agcgtgctga cggagcgcga actggatgtt ctgcacgagc
    24781 tggcacaggg gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag
    24841 taaaagtaca tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca
    24901 ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg
    24961 ggctgagatt tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    25021 gatcgatcaa tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca
    25081 tccggcataa actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc
    25141 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    25201 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    25261 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg
    25321 atattgctat ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg
    25381 ttgggatcag atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc
    25441 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    25501 atgccaacgc ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga
    25561 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg
    25621 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    25681 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    25741 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    25801 gcttccggca ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg
    25861 ccaatagcca ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    25921 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt tttactttta
    25981 ccttcctgca tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa
    26041 tcggcaatgc tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    26101 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    26161 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    26221 tctcccacag cggcttgatc ttttcttccc agacgcgagg attgagatcg cccgcatggc
    26281 gatagcgaat gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga
    26341 gatccagccc taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct
    26401 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa
    26461 tgccctgcgc tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag
    26521 tcgcccagac gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact
    26581 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    26641 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    26701 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc
    26761 cggcgcagtt ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    26821 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    26881 aagacgcacg gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    26941 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    27001 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac
    27061 accgccgcgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    27121 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg
    27181 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    27241 ataactgaag gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag
    27301 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    27361 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    27421 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag
    27481 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    27541 aatactgccc agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    27601 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    27661 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    27721 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    27781 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    27841 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    27901 cgccagtgag tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac
    27961 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    28021 cacaatgccg agcgtcagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc
    28081 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    28141 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    28201 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt
    28261 agaggtaaag agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac
    28321 acgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    28381 gctcatcagc agcacccaca gcagaagcga accttcatgc gcgccccaag tagccgccac
    28441 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    28501 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    28561 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    28621 cggatacacg gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat
    28681 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    28741 atcgctttct caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc
    28801 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    28861 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg
    28921 gtgaaggtca ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg
    28981 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    29041 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    29101 atagtcagcg ctagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    29161 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    29221 gcgccacacc acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca
    29281 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    29341 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    29401 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    29461 cagcgtggca gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    29521 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    29581 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    29641 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    29701 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    29761 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    29821 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    29881 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    29941 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    30001 gccgacggta agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat
    30061 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    30121 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    30181 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    30241 ccatcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    30301 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    30361 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc
    30421 agcgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    30481 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    30541 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    30601 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc
    30661 ggtgcaatac gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    30721 acaatcagga agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc
    30781 agttcaagac ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc
    30841 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc
    30901 cctgctccgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    30961 tggtaaaagg ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag
    31021 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    31081 gcccggcctg cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt
    31141 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    31201 gggtttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    31261 cagcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt
    31321 tgctaccggt gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata
    31381 aggttcgttc atcccgctca caaagtaact ctctggcttc aagcataccc acgcaataac
    31441 cctgttaaaa acctggctcg acttcacgca tatcgggcag cttgtgcgcg atccctttat
    31501 ggcaatcaat acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg
    31561 atttctgggc ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt
    31621 tattgtcctt catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt
    31681 tctgcggcgt gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct
    31741 tgcgtatcat cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga
    31801 cgccgctacg gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttgcgca
    31861 tttcgtggca gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc
    31921 ccccccagaa gacgatgccg ccaacaaaac cgatcaacag cagcgtcccc agcgccagat
    31981 ggctgggggt acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat
    32041 ttcccataat aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga
    32101 ttggcgcggt atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca
    32161 cttccgcgcc cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc
    32221 cagtggtgcg atagctttcg acaccgtggc actgcaagca gcgattggta ttggtcgtta
    32281 cctgataacc ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca
    32341 tccgatcctg ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg
    32401 actggctaaa atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca
    32461 tcgccgtcca ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga
    32521 actccatcgt gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca
    32581 cgtcatgcaa tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg
    32641 tagcacatgc ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg
    32701 atcgctggcc gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac
    32761 cggacaaatg tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc
    32821 gagaataagc agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagagaaac
    32881 ggggttgatc cattcccaaa tgagcgtgcc agttaaggct gatcccacca gaatgaccac
    32941 caacagcacg tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct
    33001 gcgtaaccag ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa
    33061 tcgctttccc gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt
    33121 ggcaggcaga tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac
    33181 ggtgtcgaat aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccatgg
    33241 accgctcaaa aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg
    33301 gtgactgcgc caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt
    33361 tgccattgtt cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca
    33421 gtgacagcgg taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac
    33481 cgcaaccggt acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac
    33541 gcgtgttgcg ctccagctcc aaggtgatgg cctcatcaat tttcgggcat tcgcgataac
    33601 aaacatcgca gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca
    33661 gccccatccg cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg cttgggcaca
    33721 ctttggcgca cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg
    33781 gcgtgcccgc cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag
    33841 cctgaacaca ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc
    33901 cgggcgggcg caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca
    33961 cacccacggc agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac
    34021 cattttgagg tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc
    34081 acttcttgaa gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa
    34141 ccagctgtgc ggcgtcgaag aacggcatgt acaccaggcc ctgtggcgga cggttacgac
    34201 cgcgcgtttc aacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc
    34261 cacggcgcag atcgcgcgct ttcgcatcca gcgggtgaat aaacaagacc gcttccggga
    34321 aggcgcggtg cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagaa
    34381 cgcgtccggt agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga
    34441 acggcagtgc gaagatcacc gctttgccat ccggtttacc gtagaactta tagccttcgc
    34501 ccgctttcac gtacgggtcg ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca
    34561 ccaccggcca gcgcagaccg cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac
    34621 cgtgaccgcg accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca
    34681 gctcgcggga ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca
    34741 cttcgggggt ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg
    34801 ccagcagctc ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact
    34861 gccagagatc cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac
    34921 ggcgttcggc gttaccgtaa gcgccctctt tctctaccca cattgcggtc ggcaggatca
    34981 agtcggcggc cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg
    35041 gatcgcgcca gcccggcata cgctcttcat tgatgttcgg cccggcctgc atgttgttgg
    35101 tacacatggt ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg
    35161 catgaagacc gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcacaga
    35221 tatcacgatg tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc
    35281 ccacttcacg cgcagtacca cacgcagaag gttgcccggt cagggagaac ggaccgcaac
    35341 ccggctggga aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttagcccaca
    35401 caccacgggt atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat
    35461 cggcatacag ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcagcgg
    35521 ttttttccag cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg
    35581 aggcgtcaga acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt
    35641 agccgatgtc cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta
    35701 tcgcattgtt ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg
    35761 gcgtaaagat gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca
    35821 ccgccacggt gacgttctgg ttagagagac gactgttagt gatgcgtgac cagaggatcg
    35881 ggtgcatctc cgccatgtta gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt
    35941 catagcagcc catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg
    36001 ccatacagtg acgtgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct
    36061 tggacgcggc ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt
    36121 ccggcccttt ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat
    36181 cccaggtgat tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca
    36241 gcggctgcgt caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc
    36301 agttcaggcc acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct
    36361 gctgcgttcc gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc
    36421 atttaatggc ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg
    36481 cagccgccgc agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca
    36541 tggtgtttcc tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc
    36601 ctctacgttg cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac
    36661 caccacaatc aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc
    36721 gttcagttgg gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca
    36781 aacttgccag ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg
    36841 ctgcacatgc tccacatccg ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg
    36901 agagcgtcgg gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt
    36961 caactgactg atacgccagg cacgcgtccc cgatggtgaa ctgcaaatcc caggccctgg
    37021 tgtggcgcgg agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc
    37081 actcgttatt tttgaaatta acgctcggat agccacccgt gccgcgttgc agaatgttgt
    37141 tttcacaagc attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat
    37201 caccggacca gggcggacgg ataccgttac tggctttgcg ccagcaacca gtgagtatgc
    37261 cccgacggga tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg
    37321 agagcgacca aatagaccgc ataattaata agccattttt atagccgcta agatattaaa
    37381 ggatgtgtca aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg
    37441 gcgggaagcg tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta
    37501 tatagcgatt aattcaccag agatatttct gctggtttgc tccctcatca gaatttaaca
    37561 ctaaaagagc aggtaaaatt gtctgaatgt tctttaagtt attcataaag caaattaata
    37621 aatctgatga atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag
    37681 gaaagcaaaa aaatgaagac cattctacct gaagtattgt ttgccgcttt cgctaccact
    37741 tccgcctggg cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct
    37801 gaaacaggca tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg
    37861 aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc
    37921 gggaagctgg aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa
    37981 gttagctcgc cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt
    38041 gtcaccgcgt atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg
    38101 gtgtatacgc cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt
    38161 gacaacgcgg aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc
    38221 ggttacgccg catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt
    38281 cttatcagta tacgccacat ccggcatacc atgccggatg tggcacatca ttacaacgca
    38341 atatccgcca cttctttttg aacgggttgc ggtttcggcg tactatctgc tgcttgcggt
    38401 ttgtcgtatt tcagccccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct
    38461 acatcaccgt tcagcttgcg tccataagac ggaacgatcg ctttcaacgt agcctgccat
    38521 tgcgggctgg aaacgcgatc gccaaatact ttttccagta gattcaacat aatcggcgcg
    38581 gcggttgacg cccctggcga tgcccccagc agcgccgcaa tggtcccttg ctggtcactg
    38641 accacttcgg tacccagacg cagtacgcca cctttctcgg catcacgctt gataatctgc
    38701 acacgctgcc ccgcttgcca caaacgccag tcctcttttt tcgcttgcgg atagtactct
    38761 ttcaacgctt caaaacgatc ctcttcactc aacatcacct gactcaccag atatttcacc
    38821 agatcgaaat tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa
    38881 ctcatcagat cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc
    38941 ccaaacagca ctacgcgttt accgtccaga acacgggtat cgatatgcgg aaccgacatc
    39001 ggtggtgcgc caacggatgc tttaccgtaa actttcgcca gatggcgatt aaccacgtct
    39061 ggattttccg aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc
    39121 ggaatccccg attcctgtaa cagcttcagc gccgcaccgc ccgcgccgat aaagacaaat
    39181 ttggcgcgga tgttctgtgc agtgccgttt ttcagatcgg caacggtaac ggtccaggtg
    39241 ttatcgtcat tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat
    39301 ttcttctgta aggaagcaat taactggcgg gtgatctcac catagttcac atcagtgcca
    39361 atttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt
    39421 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
    39481 cgcgcaggaa attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
    39541 taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcggg
    39601 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt
    39661 tttgcggggt gtagttcagt tccatcagtg cagaatgccc ggttccggcg ttgttctagc
    39721 cgttcgaact ctcctgcgcg cccccctcca ggcgctccac catggtcatc gaccattcag
    39781 gctccagctc gcgtcaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
    39841 gtacatcagt ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa
    39901 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
    39961 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaacact taagtaacaa
    40021 catttaaata atgtttataa attatattct aattttagaa acattataat ttcgttgatt
    40081 aattataggg ttattagaaa tgaagggatt tttagcatat tgacctgacg gcagcagtcc
    40141 gccaggtcag cgtcgtaata ttatgccgtc cgggcaacgg catcacgcga agcggcatcg
    40201 cgctcttcgc ccgtcagctc gctaagttgc ccattgcgca tttccagcag gcggtcggcg
    40261 tggataaagt aatgatcatc atgactgata gcgaaaatag ttttacccat ctcctgcatc
    40321 agcggcagca acacctgata aaactcacga cggaagtgtg gatcctgatc caccgcccat
    40381 tcatccagca ggataatatc gcgttcttct gccagcgcca gcaacagcgc cacgcgtttt
    40441 ttctgccctt ttgataactt caggttaaca atacgcccgt tgcttaactc aagcttatga
    40501 gccattttca gctgcgccag ccacttctca accagttgcg ggttagcggg ttgcccctcc
    40561 ggccccagca gttgatcaaa cagccagaca tcggtaaaca ctgccgaaaa cagtttgcga
    40621 taatcttccg gttgttcgcc gctgacaggt ttgccatcca gcaagatttc gccgctttgt
    40681 ggctgataca agcccgtcaa caacatcgcc agcgtcgatt ttccgctacc gttaccgcca
    40741 atcagaaaca gcagctcgcc acgtttgatg gtgagattaa tcggaccaac ggaaaacgcg
    40801 ttatcctgat aagcaaacgt cacgttacgc agctccagcg tttgccagtt gggaaacgcc
    40861 tgcgggcgcg gaaactcagc tttgaaaggc gcgagcgcga atttgttcag cttgttaaac
    40921 gccacctgcg ccgtcagcag cgtcggcaat gcgccaaccg ccgaaagcag cggcgtacgc
    40981 aggaataaca gcgtcaacga ataggtcgca gcaacgttgg tatcagccca accgagactg
    41041 ttcgccatcc agaacaccag gccgatagcg cccagcatca tgatgtttga ccagttcacg
    41101 gcactaagat ggaaggtgtc tgcgcgaata atatgatggc gatactcttg cgcatcgggg
    41161 atgtagaggt tgttaaacac atactcggcg cgttcccggt tcagagtcag ctctttgcgc
    41221 ccttcaagta cagtttgaaa atccgtgtac agcttgtctt cggtttcacg tagggtcgcc
    41281 atatgtttgt acacccgcgc caccagcaca aaaccgcccc agatggtgat cgccatccag
    41341 atagccgtta ccagcaacat tttgcccgac agcatccaca gatacgccga tgaaccgata
    41401 gtgagaatga tcccctgccc cagttccggc agacgcacaa aagcaatggt gatattgcgc
    41461 acatcgctgg ttaatcccgc cagcaacgag gcgctaccga gttgttcaat gcgctcgacg
    41521 tgggtatcca gaatccgctt gataaattca ctacgcagtc ggtaaacgaa gtgatgcccc
    41581 aaagtggtga gcgccagttg cgatccgaga gtgactgcca tcaacagcag caataatccc
    41641 agaaactccg gtaacaccag cagactggta tccgccgttt cgataaggcg ctgattgata
    41701 aaagcaatta agccaatgcc taatgccgca ctggcgaggc ttagcgccat cacactgata
    41761 aatggccagc gatactgccg ccagacaagt acaagaagtt ccatgcagaa aacccggaca
    41821 atgaattaca gcccgcagtt taaacatctt cgcgcgcaca gcaataataa ttcttatttt
    41881 tattcttttt tacctgcctg acggaatgtc aggttgtagc ggcagtcggt ggtgagtgga
    41941 tgaaaccccg ctttcaacgg ttgaataccg tgataaaaca gccgcgattc accgccccat
    42001 accaccacat cgccatgttc caacaacaaa cgtttgagcg gatcattacg tttcaggcca
    42061 ccaaattgaa aaatcgcggg taagcccaga gaaacagaaa caattggcgc gcgcagatcc
    42121 ggttcgtctt tatcctgatg cagcgacagt ttcgcgccag gagcgtagcg gttgataaga
    42181 caagcatctg gctggaaatc tggatagccc gccgccgtag ccgcacgttg acataaatca
    42241 tgaaagcttt gtggcatggc gggccacggt ttatttgttt gcggatcaat gggcgaatag
    42301 agataacctt gccgatgggt cgtccagccc agatgcccac agttggtcat cgccaccgac
    42361 atggtatatc cgcctggagc gaccatctgg cgaaacggcg actggctggc aacgtcatta
    42421 atatcgcgga ttagttgctc cgcagcatta aaagcaaaac gccgtaaaat taccgcacca
    42481 gccgccagtg gctcttgcca cggttcagca tcggcaaaca gatccaacat tacctctcct
    42541 cattttcagc ttcgcggcgc agcagttgcg ctttacgcga cacgccccag cggtaaccgg
    42601 aaagtgtgcc atcaccacgg accactcgat gacagggtat tacgatagcc agcttgttgg
    42661 cggcacaggc gctggcaacg gcccgtaccg ctttcggttt gccgatggcg ttagccagtt
    42721 gctgataact gacggtttca ccacaaggta tcgtgcgcag tgcctgccag acttgttgct
    42781 gaaaagcagt gccgcgaata tccagcggta acgtcagcgg cgtatcgcgt tgattgaggc
    42841 tggcgatcac ttcacgcaca tgttgctgaa acgtcagatc ggcaggcgcg ttgtcggcag
    42901 cgggaaacat ctgctgcaac tcgctgatta atgtcgcgtc atcatcgccc agcaatatcg
    42961 cgcaaatccc ccgctcgctt tctgccacca ggcaacgacc cagctcacaa tcagccagcg
    43021 cgtaacgcac cgccagattt tcgccaccgt gacggaattg tttagccgtc atgcccagcg
    43081 tttcatcggc tttgcggtag taactgctgc tgtcggggaa tccggcgtta agaatagacg
    43141 tcgtcacgct ctcccctttc gccagcgatt cgcgcaaacg gcgagcgcgc caggcctgtt
    43201 gccaggcttt aggcgtcatt ccggtagtag ctttaaacaa ccgatgtaga tgaaatggac
    43261 tcatcgccac ctggtcggct aaggcttcca gcgttacagg cgtttcctgt tccagcagtc
    43321 gacacgcgtg ggtgatttta tccaaccgat gttgccgggg attggctttg tctggctgac
    43381 aacgtttgca ggggcgaaat ccggcggcga gtgcctcgct ggcatttgcg tagaaggaga
    43441 cattttcccg caaggcatgt ctggcgcggc aagaaggacg gcaaaagata cccgtggtgc
    43501 gcacggcgaa aacgaattcg ccgtcggcat tcgggtcgcg ggctaagaca gattgccagc
    43561 gttgatcgtc agttaagcat gtggcgtttt tcataatcag ctccctggtt aaggatagcc
    43621 tttagtctgc ccggtcacca tcacgcaaaa accaacaatc ttgcgcttta atttttttcg
    43681 ctgacaagga agcttttaaa ctgtggtgac atccaggttt taaagctatc gccttctttg
    43741 gtgatcatat aaaccgccag cccttcccgg cgaacaacct ctttggcttt ctccggcccg
    43801 agtaccatca agccagtatc ccaggcatcg gcttccagcg ccgtcggagc aatcaccgtc
    43861 acggatacca gattgtgttc gatgggacgc ccggtttgcg gatcgataac atgggaaaga
    43921 cgtttgccgt ccagttcgta atagttacgg tagctgccag aggtgctaat cccatgaccg
    43981 ttgatatcca ccacagcctg aaccgcgttt tctttatcgg ttggtttttg aatcgctacc
    44041 cgccacggct ggccttcacc gttcatacca cggctgttca gcgcgccgcc caccgacacc
    44101 agatagcggg aaatcccttc ctgctccatc aagcgtgcca ggtgatccgc cgcataacct
    44161 tcaccgacgg tggagagatc gacatataaa tccggcaggt ctttttgcag atattgctga
    44221 tgcgactgat tgatgaccgt caggtgctgt aagccggttt tggctttcat cgcatcgatc
    44281 tgttcctggc tcggaatttg aaccggctgt tgttccgggc caaagcccca cagattcacc
    44341 agcggcccga cggtaatatc catcgcacca tcggtccttg ccccaatgcg cagcgaggtg
    44401 gtgacgatat cggccatcgc ttcacttacc ggccacggcg acaaactttg cgagtcgtta
    44461 aagcgcatca gcgcggaatc ttttttatag gtcgaaagca gctgatcgtc ggcgtccagc
    44521 tgggtctgaa tcttttcttt aagttcggca ctgcgtttgg catcaatgcc cgggatgctg
    44581 gcacgccaga aggtacccat ggttttgcct tcaagaacgg taacttcagt agcgtgggtt
    44641 ttggcgggtt gtggtttttg atcgcaacca acaaagaaga gcgcggcaac cagcagtgcc
    44701 acgcgggtaa agcttatttc catacgtgat tatcctcatg cgaacggtcg caagagtaca
    44761 ccaaaaaact gtgtttgtac gctgaaaaca atgaaaaaag ggcccgcagg ccctttgttc
    44821 gatatccatc gagattagaa ctggtaaacc agacccagag ctacgatgtt atcagtgttg
    44881 atgccagcgt cacgagtgaa ctggttgtcg tccagcaggt tgattttgta gtcaacgtag
    44941 gtggacatgt ttttgttgaa gtagtaggtc gcaccaacat caacatattt caggatatct
    45001 tcgtcgtcgt agttacgacc attgatgaca cccaggtttt tacctttaga ctgcaggtaa
    45061 gccagggacg gacgcagacc gaagtcgaac tggtactgag caacagcttc gaagttctgt
    45121 gctttgttcg cccaacccaa gttgcctacg cgagttgcgt tgtaggtctg ggtgtactga
    45181 gcagctaggt agatgttgtt cgcgtcgtat ttcagaccac cggtgtaggt ttcagcgcgg
    45241 tcgccattac cgatgtaacg ctcatcataa cggttcagac cgaagttctg gtcatcagta
    45301 cgtttggagc tggaaactgc agcaccgata ccgaagcctt cgtaatcata agtaatagaa
    45361 ccgccaacgc cgtcaccgtt ctgacgcaga gcttcacgac cgttgttggt catgccttca
    45421 ccttccgggc taccgttttt gccctgatac tgaacagcga agttcagacc gtcaaccaga
    45481 ccaaagaagt cggtgctacg gtaggttgcg aagccgttac cacgctgctg catgaagttg
    45541 tcagaaccgt aagtgtcacc accgaattct ggcagtacgt cggtccagga agttacgtcg
    45601 taaactacgc cgtagttacg accgtagtcg aaagaaccta catcctggaa tttcagacct
    45661 gcgaatgcca cacgggtcca ggagttgttt tcgttttcag cgctgttgcc ctggatctga
    45721 tattcccact ggccgtaacc ggtcagctgg tcagtaacct gagtttcacc tttgaagcca
    45781 agacgcatgt aggtctggtc gccatctaca gacttgtcgt cagagaaata gtgcaggccg
    45841 tctactttac cgtacagatc taatttgttg ccgtctttgt tgtaaacttc agcagcgttt
    45901 gctgcgcctg ctaccagcag agctgggacc aggagggaca gtactttaac tttcatgtta
    45961 ttaaccctct gttatatgcc tttatttgct tttttatgcc actgcatact gattaaccct
    46021 cattaatcag tcggcaagtc cattctcccc aaaaatgcag aataatccaa cacgaatatg
    46081 atactaaaac ttttaagatg tttcatttat cgctatagat gtttcaaaat gtaaatacaa
    46141 gggaactttt taagattatt gcggaatggc gaaataagca cctaacatca agcaataata
    46201 attcaaggtt aaaatcaata acttattctt aagtatttga cagcactgaa tgtcaaaaca
    46261 aaaccttcac tcgcaactag aataactccc gctatcatca ttaactttat ttattaccgt
    46321 cattcatttc tgaatgtctg tttaccccta tttcaaccgg atgcctcgca ttcggttttt
    46381 ttacccttct ttacacactt ttcattattc tgtgctacca cagaaaaact ataacgcttg
    46441 ttaactattt cacaaataat taacatcagc ataatttcca gcaatctttg tttatttgca
    46501 attaatttcg ttgggctttt tgtaggttat ttgtacagca aaatggcgct tgtacatcta
    46561 tttcccccca tacaggatga taaatatcac gggagaatag agaatcatca atcaggtaag
    46621 agtctggaat ttcacactgt accctttata ctgccctatc acttcgcgaa gttttaacag
    46681 gtcataaaca cgaatgcgtc agaaagagac aacggccacg acccgctttt cactcctacc
    46741 ggggagcatt acccgcttct ttttactgtt gatcattgtg ttactggtga cgatgggggt
    46801 aatggtacaa agtgccgtta acgcttggct gaaagataaa agttaccaga ttgtcgacat
    46861 tacccacgca atccaaaagc gcgtcgatac ctggcgttac gtgaccaggc agatctacga
    46921 caacattgcc gcgacgacct ccccctcctc cggcgaaggt ttacaagaga cgcgcctgaa
    46981 acaggatgtc tactatctgg agaaaccacg ccgcaaaacg gaagcgttaa tctttggctc
    47041 tcacgacaac tcaacgcttg agatgactca gcggatgtcc acttatctgg atacattgtg
    47101 gggtgcagaa aatgtaccgt ggtcgatgta ttacctgaat ggtcaggata acagtctggt
    47161 gctgatctca accctacccc ttaaagatct cacctccgga tttaaagaat cgaccgtcag
    47221 tgacattgtt gattcacgtc gtgcagaaat gttacaacag gccaacgccc tcgatgaacg
    47281 cgaaagtttt tctaacatgc gccgcctagc ctggcagaac ggtcattact ttaccttgcg
    47341 taccacgttc aaccagccgg gacatctggc aacggtcgtg gcttttgatc tgccgattaa
    47401 tgatttgatc ccaccgggta tgccgctgga cagtttccgc cttgagccag acgcgacggc
    47461 aacgggaaac aatgataatg agaaagaagg gacggatagc gtcagtatcc actttaacag
    47521 tacgaagatt gaaatctcct cggcactcaa ctctaccgat atgcgcctgg tctggcaggt
    47581 tccttatggc accttattgc tggatacgtt gcaaaacatt ctgctgccac tgctgctgaa
    47641 catcggtttg ctggcgctgg cgttatttgg ctataccaca ttccgccatt tctccagccg
    47701 cagtacagaa agcgtcccca gcacggcggt caataacgaa ttgcgcattt tacgggcaat
    47761 caatgaagag atagtctcac tgttgccgct cggcctgctg gttcacgatc aggaatcgaa
    47821 ccgcactgtc ataagtaaca aaattgccga tcatttgctg ccgcatctga atctgcaaaa
    47881 catcaccacc atggcggaac agcatcaggg gattattcag gcgacgatca ataacgagct
    47941 gtatgagatc cgcatgttcc gcagccaggt cgcgccgcgc acacaaattt tcattattcg
    48001 cgatcaggat cgcgaagtgc tggtaaacaa gaaactcaag caggcgcagc gtctgtatga
    48061 gaaaaaccag caggggcgga tgacctttat gaaaaacatt ggcgatgcgc tgaaagaacc
    48121 cgcacagtcc ctggcggaga gtgcggctaa actcaacgcc ccggaaagca aacaactggc
    48181 gaatcaggca gatgtgctgg tgcggctggt cgaagaaata cagttagcga acatgcttgc
    48241 ggatgatagc tggaaaagtg agacggtgct gttctccgtg caggatttaa ttgatgaagt
    48301 tgtgccttca gtgttgcctg ccatcaagcg taaaggtctg caactgctga ttaacaatca
    48361 tctgaaagca cacgatatgc gccgcggcga tcgcgatgcc ttacgacgta ttttgctgct
    48421 actgatgcaa tatgccgtga cctcaacgca attgggaaaa atcacccttg aggttgatca
    48481 ggatgagtcc tctgaagacc gcctgacatt ccgcattctg gacacgggag aaggcgtaag
    48541 tattcatgaa atggataatt tgcacttccc gtttatcaac cagacccaaa acgatcgtta
    48601 tggaaaggcg gacccgctgg cattctggct gagcgatcaa ctggcacgta aactgggcgg
    48661 tcatttaaac atcaaaacgc gggatgggct tggtacacgc tactctgtgc atatcaaaat
    48721 gctcgccgct gacccggaag ttgaagagga agaagagcgt ttactggatg atgtctgcgt
    48781 aatggtggat gttacttcgg cagaaattcg gaatattgtc actcgcctgt tagaaaattg
    48841 gggtgcaacc tgtatcacac ccgatgaaag attaattagt caagattatg atatcttttt
    48901 aacggataat ccgtctaatc ttactgcctc tggcttgctt ttaagcgatg atgagtctgg
    48961 cgtacgagaa attgggcctg gtcaattgtg cgtcaacttc aatatgagca acgctatgca
    49021 ggaagcggtc ttacaattaa ttgaagtgca actggcacag gaagaggtga cagaatcgcc
    49081 tctgggcgga gatgaaaatg cgcaactcca tgccagcggc tattatgcgc tctttgtaga
    49141 cacagtaccg gatgatgtta agaggctgta tactgaagca gcaaccagtg actttgctgc
    49201 gttagcccaa acggctcatc gtcttaaagg cgtatttgcc atgctaaatc tggtacccgg
    49261 caagcagtta tgtgaaacgc tggaacatct gattcgtgag aaggatgttc caggaataga
    49321 aaaatacatc agcgacattg acagttatgt caagagcttg ctgtagcaag gtagcctatt
    49381 acatgaacaa tatgaacgta attattgccg atgaccatcc gatagtcttg ttcggtattc
    49441 gcaaatcact tgagcaaatt gagtgggtga atgttgtcgg cgaatttgaa gactctacag
    49501 cactgatcaa caacctgccg aaactggatg cgcatgtgtt gattaccgat ctctccatgc
    49561 ctggcgataa gtacggcgat ggcattacct taatcaagta catcaagcgc catttcccaa
    49621 gcctgtcgat cattgttctg actatgaaca acaacccggc gattcttagt gcggtattgg
    49681 atctggatat cgaagggatc gtgctgaaac aaggtgcacc gaccgatctg ccgaaagctc
    49741 tcgccgcgct gcagaaaggg aagaaattta ccccggaaag cgtttctcgc ctgttggaaa
    49801 aaatcagtgc tggtggttac ggtgacaagc gtctctcgcc aaaagagagt gaagttctgc
    49861 gcctgtttgc ggaaggcttc ctagtgaccg agatcgctaa aaagctgaac cgcagtatta
    49921 aaaccatcag tagccagaag aaatctgcga tgatgaagct gggcgtcgag aacgatatcg
    49981 ccctgctgaa ttatctctct tctgtgacct taagtccggc agataaagac taatcatctg
    50041 taggccagat aagacgcgtt agtgtcttat ctggcatttg caccgattgc cggatgcggc
    50101 gtaaacgcct tatccggcct acgattccca ttatttcaac agattacatt aaagtaggcc
    50161 agataagacg cgtcagtgtc ttatctggca tttgcactga atgccggatg cggcgtaaac
    50221 gccttatccg tcctacgaat cccgcgattt cctgaccctc tcggcatata ccgtcagcgt
    50281 ctgttttatc acatccagcg ttaccggctt cgagaggcag ctgtccatac cggactccag
    50341 acaccgctgc ttctcttcag ccaacgcatt agcagttact ccgattaccg gcaacgtcag
    50401 tcccaactga cgaatgcgtt gcgtcaagcg gtaaccatcc atatttggca tgttgacgtc
    50461 gctaagcacg atatcaatat gattcttact aagtacatta agcgcatcga cgccatcatt
    50521 cgcggtttta cattgatagc ccaacgatcc caactgatct gccagcagac gtcggttaat
    50581 cggatgatca tccacgacca gaatcatcat atcgtcgtta tcgctgaccg ctttgtccgt
    50641 cgacggcaga gcgttagcag gatcgtcgct ctccatctcg atcaaataaa tacgcgccaa
    50701 caatgccggt agctcatgcg gagcagccac actgtgtacc cactcccctg gcgctttctc
    50761 cagcggaata ccaatatggc gacgacagaa ggtcactacc gctctgccct gccatttttt
    50821 actcactacc tcgtcagtga ttaacacatc ttcgggagtc ggttcctgcc cttcgtatgt
    50881 tgtaacgacg atgccgctgc gctgcaaact ggtttccagg aactgacaga gcgacgcatt
    50941 gcggaccgcc agccagcagc gtttaccact caacccttcc acgccttttt tctgcgggta
    51001 ctgagcgccg tacaacggaa tacgcacggt aaactggctg cccattcccg gttctgaatc
    51061 taccgagata tcgccgtcca tcatgctgat cagtttttca caaatcgcca gacccagacc
    51121 ggtcccctgg aagtttcgct gtacgcccgt tccaacctgg aagaagggat caaacaagcg
    51181 caccacttct ttcgccggaa tccccacgcc ggtatcgcga acacggatag agagataatc
    51241 gccatcagcg cgaacatgca aaactataca gccggtatcg gtgaatttta tggcgttact
    51301 caacaggttg gagatgacct gctgtaaacg catcgggtcg ccatttaagg ccactggcac
    51361 atccggttca ataaagcagt acaagcctaa ctgcttgcgt accaccagcg gtaaatagtt
    51421 ggcggtgatg tggttcatca cttcacgcgg tgaaaactca cgcggttcga tcttcaactg
    51481 ttccgattca atcttcgaga aatcgagaat atcgctgata attttcaaca acaggctgga
    51541 agagttgttc attaccgtca ccagacgatc gacgcctttc ggtaactctt tggtttgcaa
    51601 cagatccagg ttaccgataa tgccatacag cggcgttcgc agctcatgac tgacggtggc
    51661 aaggaacatc gattttgact ggctcgcctg ttccgctgct tgtgccatct cctgcaacga
    51721 ctcttccatc ttcacgcgcg aagaaacatc caccagcaca caaatggcca cgttttcatt
    51781 acgatagcgc gaatggacga agctgatttg cagattggta ttgttgctgg tcaggacatc
    51841 aacaaaattg acctgctgcc cacagataat ttgcgtcagt cgttggcggt cctcatgcgt
    51901 aagcatattg agataggtat gcgccagttc gttacttaaa atattgacgc catcagcggt
    51961 acgcaaaatg cagataccca ctggcgcgga ggcgacaatc ttgcgattga actgctcatg
    52021 ttcttccagt cgcagggcgt cgctttccgc cggaatgaaa atacgtcgct cgtacatccg
    52081 cgcgagagta aacaatgcag ctccggcaag cacattcagc aaaattgcgt taaggatcaa
    52141 catgcgaatg cgttccagca ccttatcaac cggcaccgaa tacacgatgc ttagcgatga
    52201 gggtggcaga tttttcttca gcaccagctc ccggaaccct tccgtatagc caaaccagga
    52261 gcgttcctgc atccagcgag gatcgccctt aattttactt tctggtccgg taagcgaaat
    52321 cagggtatga ccattttcat caagaatggt aacccccatc ggcaacgtac ccggtaagaa
    52381 aaagttctcc atccggatgg tctgctcgac acccaaaagc gcctgcaacc ggttcgccag
    52441 ataaactggc gtcaacgcgt aaaaataccc gacgcaaggg cgcggacctt cgctgatcca
    52501 gtagaggtta ctgccgctat catcttgtgg tgcatttcga tatttattga tgcgttcatg
    52561 caacgctttc aacgcggtat cgcgttccac tggcatatca cgcagaccga aattggccat
    52621 gcagaggtta tcactgccga ttaaaaatac ccggttgaga tcgtaagccg cagaaaaatt
    52681 atcgcgccag tagcgcataa accacgccaa tgactccaga gaacctcgcc aggtgttact
    52741 cattgcggaa caatcggagt cggcaaacag cggttcaaac gcaggcacat ccgcctgcgt
    52801 ttctcgtcca cgcggggaaa gcacaccgtt ttctgccgat aagcgatttt cggcgatgta
    52861 cttaagctct ttcatcacat cagaggtgcg ttgaataaag cgctgagcct gatcggaact
    52921 cagattaaat tcctgacgaa tttccgattc tcgctgatgt aacgcattaa cgatgtaaaa
    52981 aacggatgaa aaagcaatca acagccagag cactaacgcc aatgctctga acatgtagcg
    53041 cgaggctttc agggttgtac gaaaagaagc aaggtatttc aaaggggaga agctccgcct
    53101 caggtgaccg atggagtgtg gttaaggtag cggtaaaagc gtgttaccgc aatgttctgt
    53161 caccattgca ataatgcgta tcgcttagaa accattgcct taaccaggaa agccggagta
    53221 gatggacagt tctcagtacg aaaaataaga aatgaccaat ctgccgacgc ttgttaatgg
    53281 ttatatcctg gcgtcggcag atcgcccagc agtcttatct gaaaaaagag agagccaggt
    53341 tgtgtgaagg gatggatcac cagtcaaaac ttttcaccag cgtcagctcg ccagcattac
    53401 gcatcggtac aataaatgtt tcctgtttct cattgaccga tccttcatcg gtgatcagcg
    53461 tcagttgggc ggtcgtcaat tccgtttcgc tgcgcccacc atagtagttg atatacacct
    53521 gatagcgccc gtgaactggt gctggcatgg cgaaaatctc gggtccgtac cccgtcgtga
    53581 catccatatc cagtgcacca ctgtttttca gcacggtgtt accgtaccag gcgtgttcgc
    53641 catcgggcgt gacaacatgg agatcgagat cggtattgtc cgtatcccac gagagaacca
    53701 gccgtaaacg tgcacgaata gttcctgtac ccggcgttga gtaaaactgc attttttgcc
    53761 ggctttgccc atccgggctg atgacctgca cgctgttgct gccttcagtg aaaatataag
    53821 ggcgtgcaaa tgaaccatcg gattcaattc tctgcggcat actggcaccg ttgaccacca
    53881 gccggccctg ctgaacttta cccgccgcag cataattctt tattttgccg cggatctgcg
    53941 ctgagatatt ttgatcatct gccatgttga cggacgacgc cgggtaattg atcgattgtg
    54001 aaaaactggc atcttcgccc tctgccggat gccagccaga gagcggtgcg tcaatctcga
    54061 cttcctgcac accttcactg tgagcgacag gcgaaagtgc caccagtaac aacggaagaa
    54121 aaatctttcg catagcatta gtccattaat aattgtcggg tcagcgtttc gatatagttc
    54181 tcatcaagcc cggtgggata accatcaaag gccaagtgca gatactcatg ggttaaatcc
    54241 agccgatcct gaagcgtaaa gaagttacga ataaacagcc gtttctgctg ccgatcggta
    54301 taggggaaac ctgagactaa acgacagacc gcaaacacgt ctggttcgtt gtaccccgtc
    54361 tcgccttgta atatacgccg ccactgcggc atttttttcg ccagccaggc tttcgctttg
    54421 ggtaataact ggcaggttga gcgtggagct ccccagcggc taaggctgtt gtcgggataa
    54481 gcaaacgcga gtatctggtc gtaacgctcc ccctgccccg cctgggccgt ggcttgccgc
    54541 caggaaagtg tcccttcggt agcgcgactg ccatgataat gaaccggatc gcctgcatag
    54601 atgagctcct gcgtccaggc ggtcatcgtt cgcgcccccg tggtcgctgg cgaagcagaa
    54661 acacgctgcg tggcgctgct gtcggggatg gtcaggcaat caccttcccg attggcgttt
    54721 tgctgtaaaa aagtacgtat cgccacagtc atggctttcg ctgcctcagg tggcgtactt
    54781 ttcgcctcgc gatccagaac tctggtaacg tactcttcac gatcgagatg cgactgcaat
    54841 ttcagtttgc ccttttcgct cagtaaggtc gtctcaccgt ggctgacaaa cgtaatgtgg
    54901 ttaccattag taaacgtcac acgatagcgc ccattaagta cgctgggttt tactgccgtc
    54961 gtactctttt ccgctgtgat tttcttcagc ggatagcggg caaacagctc cacctctaca
    55021 cattgcccac tggcaacctg cgtcggcact ggcaaaaccc gattaagcac cgtcgcataa
    55081 cgggttagta ccgttttgct ggtgccactg ccagtaaccc acagcggtgt accatcggtt
    55141 aaccagccag caaaaccacc ctgacggatt tcctgtttat catcggcaaa ccagctccag
    55201 gttttcaccc gtacccgact accgagcatg gatgcgacac cgattttcgc ctcgtccagc
    55261 accacatcca gtaagacttc ctgagctttg ttttgcgcag gtaacgtcgc taatgaatca
    55321 agcagcgatt taaccgttac cgaggtttct ggcttcagcg ttgtaagaga cgctaaccac
    55381 gcaggtgcct gacgctgttg ccagtattgc ccccacacat ctgcgccaat atgcaaccgc
    55441 tgcggcgcaa aatagagccc acaggaacgt accagcgcgg tatcgcgcgt tatgctctcc
    55501 ccagcctgac aacaataaac ctcttccggg gagtttccgc gacactggta gggctgctcc
    55561 ggttgatggg tatcctccag ccaggcgtaa acatacagtt tccacaaact ccctaacggc
    55621 gtggtgagcg agtcaggtaa tgctgagact ttggtcacgc ctgatgacga cagctgatac
    55681 agttgatcgt gctgcgcacc gcgcagcacc agttgtaacg gcgtctcctc cgcaagcgtt
    55741 ggcagagtaa ccaacgccaa tagccagacg attctgcgcc agttcactta tttcacctgc
    55801 atcccggtcc attcactccc tgccgcgaca ctttgctgcg caggtgcata ggaacgcaca
    55861 taacgagcag gaggcagaac gaattgccct ttttgcgaga agcgcagcaa atggcggaaa
    55921 gtgaccgttc ccgtcagttc tttcaccggc accatatagg ccagttcgcc catttcatta
    55981 cgcgcttttt ccagcaattg cccctgctgt ttcgcggcgt tgggtttatt gactgaaatg
    56041 ccccatgttg tgcgctcaac gtcggctccc ggcgggagcg gtacttccac ctgaccatag
    56101 cgcagaactg catcctgctc gctggtaagc gtaatttcat cgagatacag cgcatcgctg
    56161 tcaatctcat tgctggtcac cggctgcaga gtaaagctca tctcttcttc accggggata
    56221 agccgataca actggcgttc aacggtcacc ggaatgttac tttgttgagc cgtttttgcc
    56281 ggttcacgcc agcggacctg cacatttgcg gcgataattc gtcaccaaaa gagagaatgt
    56341 ccggcacacc ctaaccaacc caacgccagt cttcgccccc tccagttaac ttatgtttag
    56401 cccatgcgcc cgcaggcgca ggcaaaacaa ctggaggcat tgtcgccata tatttcgcca
    56461 gccagttcat ggccagcgcg cgctcaatag tggattgctc agcggtcaaa ccattaaaat
    56521 agcggccgtc tgggtagcgt caccaccgct attgagcagc acaaccgtgt gcgccagcgg
    56581 ctgattgctg tttgccgctt tatcccacgc atattgctga acctgtggca tcaccgtgga
    56641 cttcaactgc gcttttttca gcaacgtcat ggttaagacg tttgccaccg catctgccag
    56701 tggagattcc ggtgtatcga ggatcaggct gtcattgata tcgcggatgt cttcctcaga
    56761 gaaatcttca tctttagttc cgcgccgggc gatagcttca tccccccctt tcaacaacgt
    56821 tttgcacggc agattcatct cctgtgccca cgccagcacc agcgcccgat gcaataacgg
    56881 catgttatcc gcctgctcgg cataactgtc gagcatatgc tgccagtatt ccggttgttg
    56941 cgttacgccg agcgcctggc tggcctgcca gtcggcgtac catgcccatg ccgtaaggaa
    57001 ggcgtcacca ttgccatctt caccccacca ggtaaagcgc gctccgggcc ccgccagttg
    57061 catcagccgc agacggttat cctgaatcat ctgacgaatg tcgttagcgg cggcactttg
    57121 atgatcggca agcgaacgcc aggcgagact gagcgggatc agacggctac cggtgttgat
    57181 caccccaccc cacggttcat cgactaacgc atcaagattg ttgcggaaaa tctcctgcgg
    57241 cgtttcacta ctttgtagcc ggatattact cgcctgctcg ggcaacatca gcgcgttctc
    57301 gccaccgccg agcatgacat ttttctgctg ttcaacgggc cagctgttgt ccacaaaaga
    57361 cagttttgtg ctaatgctgt cctgcacttg cccattttgt tgcagttctg cacttaacaa
    57421 gccagattgc tgaatattct gcgtcagcga aatataattc gcccctttgt gcagcgtcag
    57481 cgtctggcgc atctcagcgc ctgcaaattt agtcaccagc gccaccggtt cgttatcctg
    57541 ctgactgaag ataaacagtc ctgccgccgg tttgtcgccc acgcgataca ccgttggcat
    57601 actccacttc atgtagagat ttttttccga acgcagataa gcacgcccct gcccgaccag
    57661 cccgtcgccg ttcatcccac gcgcggtgat acgccagcgg gttaacgaat caggcatcag
    57721 gaacgtgaaa tacgctttgc cttgtttatc ggttgtgagt gacggcatcc atgccgcggt
    57781 atccacctct tcacgccgtg gacgttcagg catttttact cgccgctcgc tgcggttagt
    57841 cgcgccaggc gcaaccggct cgctagagag cgcctggtca tagctgataa acgacagact
    57901 ggagctggta cgcacattgt tacgccccag cggatagaaa aatttgccga tattcggcgc
    57961 gatttctggt tgcagcgcgt agatcatttc atcgaccacg cctaccgtta gctgcgcaga
    58021 aacaggttta cctttcagcg acgaggttaa tt
//
LOCUS       DAJTNW010000016        55430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNW010000016 DAJTNW010000000
VERSION     DAJTNW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO179_001015"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO179_001015"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5531169.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO179_001016"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO179_001016"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5531170.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO179_001017"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO179_001017"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5531171.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO179_001018"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO179_001018"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5531172.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO179_001019"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO179_001019"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5531173.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO179_001020"
     CDS             7826..9559
                     /locus_tag="OO179_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5531174.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO179_001021"
     CDS             9708..10559
                     /locus_tag="OO179_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5531175.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO179_001022"
     CDS             complement(10546..10887)
                     /locus_tag="OO179_001022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5531176.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO179_001023"
     CDS             complement(10889..11767)
                     /locus_tag="OO179_001023"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5531177.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO179_001024"
     CDS             complement(11733..14030)
                     /locus_tag="OO179_001024"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5531178.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO179_001025"
     CDS             complement(14081..14401)
                     /locus_tag="OO179_001025"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5531179.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO179_001026"
     CDS             complement(14416..15495)
                     /locus_tag="OO179_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5531180.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO179_001027"
     CDS             15804..16907
                     /locus_tag="OO179_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5531181.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO179_001028"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO179_001028"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5531182.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO179_001029"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO179_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO179_001030"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO179_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="OO179_001031"
     CDS             20366..20983
                     /locus_tag="OO179_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5531183.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="OO179_001032"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="OO179_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5531184.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="OO179_001033"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="OO179_001033"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5531185.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="OO179_001034"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="OO179_001034"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5531186.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="OO179_001035"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="OO179_001035"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5531187.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="OO179_001036"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="OO179_001036"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5531188.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="OO179_001037"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="OO179_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5531189.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="OO179_001038"
     CDS             30130..30738
                     /locus_tag="OO179_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5531190.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="OO179_001039"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="OO179_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5531191.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="OO179_001040"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="OO179_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5531192.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="OO179_001041"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="OO179_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5531193.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="OO179_001042"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="OO179_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5531194.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="OO179_001043"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="OO179_001043"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5531195.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="OO179_001044"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="OO179_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5531196.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="OO179_001045"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="OO179_001045"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5531197.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="OO179_001046"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="OO179_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5531198.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="OO179_001047"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="OO179_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5531199.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="OO179_001048"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="OO179_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="OO179_001049"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="OO179_001049"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5531200.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="OO179_001050"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="OO179_001050"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5531201.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="OO179_001051"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="OO179_001051"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5531202.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="OO179_001052"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="OO179_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5531203.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="OO179_001053"
     CDS             complement(44853..45152)
                     /locus_tag="OO179_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5531204.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="OO179_001054"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="OO179_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="OO179_001055"
     CDS             45905..46504
                     /locus_tag="OO179_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5531205.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="OO179_001056"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="OO179_001056"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5531206.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="OO179_001057"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="OO179_001057"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5531207.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="OO179_001058"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="OO179_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5531208.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="OO179_001059"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="OO179_001059"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5531209.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="OO179_001060"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="OO179_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5531210.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="OO179_001061"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="OO179_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5531211.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="OO179_001062"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="OO179_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5531212.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="OO179_001063"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="OO179_001063"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5531213.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="OO179_001064"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="OO179_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5531214.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13505 a        15020 c        14161 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAJTNW010000017        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNW010000017 DAJTNW010000000
VERSION     DAJTNW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(11..322)
                     /locus_tag="OO179_001065"
     CDS             complement(11..322)
                     /locus_tag="OO179_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5531215.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO179_001066"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO179_001066"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO179_001067"
     CDS             701..1054
                     /locus_tag="OO179_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5531216.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO179_001068"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO179_001068"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5531217.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO179_001069"
     CDS             complement(2810..3280)
                     /locus_tag="OO179_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5531218.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO179_001070"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO179_001070"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5531219.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO179_001071"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO179_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5531220.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO179_001072"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO179_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5531221.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO179_001073"
     CDS             complement(5283..8432)
                     /locus_tag="OO179_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5531222.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO179_001074"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO179_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5531223.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO179_001075"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO179_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5531224.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO179_001076"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO179_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5531225.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO179_001077"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO179_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5531226.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO179_001078"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO179_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5531227.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO179_001079"
     CDS             14911..15288
                     /locus_tag="OO179_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5531228.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO179_001080"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO179_001080"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5531229.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO179_001081"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO179_001081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5531230.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO179_001081"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO179_001082"
     CDS             18099..18428
                     /locus_tag="OO179_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5531231.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO179_001083"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO179_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5531232.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO179_001084"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO179_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5531233.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO179_001085"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO179_001085"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5531234.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO179_001086"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO179_001086"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5531235.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO179_001087"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO179_001087"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5531236.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO179_001088"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO179_001088"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5531237.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO179_001089"
     CDS             complement(25481..27157)
                     /locus_tag="OO179_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5531238.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO179_001090"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO179_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5531239.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO179_001091"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO179_001091"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5531240.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO179_001092"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO179_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5531241.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO179_001093"
     CDS             complement(31205..31999)
                     /locus_tag="OO179_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5531242.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO179_001094"
     CDS             32137..32478
                     /locus_tag="OO179_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5531243.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO179_001095"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO179_001095"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5531244.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO179_001096"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO179_001096"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5531245.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO179_001097"
     CDS             36394..37686
                     /locus_tag="OO179_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5531246.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="OO179_001098"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="OO179_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5531247.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="OO179_001099"
     CDS             complement(38219..38677)
                     /locus_tag="OO179_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5531248.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO179_001100"
     CDS             complement(38674..39591)
                     /locus_tag="OO179_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5531249.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO179_001101"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO179_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5531250.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO179_001102"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO179_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5531251.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO179_001103"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO179_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5531252.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO179_001104"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO179_001104"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5531253.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO179_001105"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO179_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO179_001106"
     CDS             43550..44236
                     /locus_tag="OO179_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5531254.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO179_001107"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO179_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO179_001108"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO179_001108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5531255.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO179_001109"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO179_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5531256.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OO179_001110"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OO179_001110"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5531257.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OO179_001111"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OO179_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5531258.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OO179_001112"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OO179_001112"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5531259.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53211
                     /locus_tag="OO179_001113"
     CDS             52938..>53211
                     /locus_tag="OO179_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5531260.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12829 a        13692 c        14020 g        12670 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 tgcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatctcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt c
//
LOCUS       DAJTNW010000018        51887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNW010000018 DAJTNW010000000
VERSION     DAJTNW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(78..2357)
                     /gene="maeB"
                     /locus_tag="OO179_001114"
     CDS             complement(78..2357)
                     /gene="maeB"
                     /locus_tag="OO179_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5531261.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2646..3596
                     /gene="tal"
                     /locus_tag="OO179_001115"
     CDS             2646..3596
                     /gene="tal"
                     /locus_tag="OO179_001115"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5531262.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3616..5619
                     /gene="tkt"
                     /locus_tag="OO179_001116"
     CDS             3616..5619
                     /gene="tkt"
                     /locus_tag="OO179_001116"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5531263.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5722..6765)
                     /locus_tag="OO179_001117"
     CDS             complement(5722..6765)
                     /locus_tag="OO179_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5531264.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6891..7466)
                     /gene="nudK"
                     /locus_tag="OO179_001118"
     CDS             complement(6891..7466)
                     /gene="nudK"
                     /locus_tag="OO179_001118"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5531265.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7534..9513)
                     /gene="aegA"
                     /locus_tag="OO179_001119"
     CDS             complement(7534..9513)
                     /gene="aegA"
                     /locus_tag="OO179_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5531266.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9719..11436
                     /gene="narQ"
                     /locus_tag="OO179_001120"
                     /pseudo
     CDS             9719..11436
                     /gene="narQ"
                     /locus_tag="OO179_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11638..14751
                     /gene="acrD"
                     /locus_tag="OO179_001121"
     CDS             11638..14751
                     /gene="acrD"
                     /locus_tag="OO179_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5531267.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14850..14909)
                     /gene="ypfM"
                     /locus_tag="OO179_001122"
     CDS             complement(14850..14909)
                     /gene="ypfM"
                     /locus_tag="OO179_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5531268.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15290..15646
                     /locus_tag="OO179_001123"
     CDS             15290..15646
                     /locus_tag="OO179_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5531269.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15650..16777
                     /gene="dapE"
                     /locus_tag="OO179_001124"
     CDS             15650..16777
                     /gene="dapE"
                     /locus_tag="OO179_001124"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5531270.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16805..17005
                     /locus_tag="OO179_001125"
     CDS             16805..17005
                     /locus_tag="OO179_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5531271.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17115..17813)
                     /gene="ypfH"
                     /locus_tag="OO179_001126"
     CDS             complement(17115..17813)
                     /gene="ypfH"
                     /locus_tag="OO179_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5531272.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIALNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(17887..19902)
                     /gene="tmcA"
                     /locus_tag="OO179_001127"
     CDS             complement(17887..19902)
                     /gene="tmcA"
                     /locus_tag="OO179_001127"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5531273.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(19917..20780)
                     /locus_tag="OO179_001128"
     CDS             complement(19917..20780)
                     /locus_tag="OO179_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5531274.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(20948..21661)
                     /gene="purC"
                     /locus_tag="OO179_001129"
     CDS             complement(20948..21661)
                     /gene="purC"
                     /locus_tag="OO179_001129"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5531275.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(21874..22908)
                     /gene="bamC"
                     /locus_tag="OO179_001130"
     CDS             complement(21874..22908)
                     /gene="bamC"
                     /locus_tag="OO179_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5531276.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(22925..23803)
                     /gene="dapA"
                     /locus_tag="OO179_001131"
     CDS             complement(22925..23803)
                     /gene="dapA"
                     /locus_tag="OO179_001131"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5531277.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            23949..24521
                     /locus_tag="OO179_001132"
     CDS             23949..24521
                     /locus_tag="OO179_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5531278.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     TFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24521..24991
                     /gene="bcp"
                     /locus_tag="OO179_001133"
     CDS             24521..24991
                     /gene="bcp"
                     /locus_tag="OO179_001133"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5531279.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25244..25861
                     /gene="hyfA"
                     /locus_tag="OO179_001134"
     CDS             25244..25861
                     /gene="hyfA"
                     /locus_tag="OO179_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5531280.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            25861..27878
                     /gene="hyfB"
                     /locus_tag="OO179_001135"
                     /pseudo
     CDS             25861..27878
                     /gene="hyfB"
                     /locus_tag="OO179_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            27889..28836
                     /gene="hyfC"
                     /locus_tag="OO179_001136"
     CDS             27889..28836
                     /gene="hyfC"
                     /locus_tag="OO179_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5531281.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            28853..30292
                     /gene="hyfD"
                     /locus_tag="OO179_001137"
     CDS             28853..30292
                     /gene="hyfD"
                     /locus_tag="OO179_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5531282.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30304..30954
                     /gene="hyfE"
                     /locus_tag="OO179_001138"
     CDS             30304..30954
                     /gene="hyfE"
                     /locus_tag="OO179_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5531283.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            30959..32539
                     /gene="hyfF"
                     /locus_tag="OO179_001139"
     CDS             30959..32539
                     /gene="hyfF"
                     /locus_tag="OO179_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5531284.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            32529..34243
                     /gene="hyfG"
                     /locus_tag="OO179_001140"
                     /pseudo
     CDS             32529..34243
                     /gene="hyfG"
                     /locus_tag="OO179_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34253..34798
                     /gene="hyfH"
                     /locus_tag="OO179_001141"
     CDS             34253..34798
                     /gene="hyfH"
                     /locus_tag="OO179_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5531285.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34795..35553
                     /gene="hyfI"
                     /locus_tag="OO179_001142"
     CDS             34795..35553
                     /gene="hyfI"
                     /locus_tag="OO179_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5531286.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35546..35959
                     /gene="hyfJ"
                     /locus_tag="OO179_001143"
     CDS             35546..35959
                     /gene="hyfJ"
                     /locus_tag="OO179_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5531287.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            35989..38001
                     /gene="hyfR"
                     /locus_tag="OO179_001144"
     CDS             35989..38001
                     /gene="hyfR"
                     /locus_tag="OO179_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5531288.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38023..38871
                     /locus_tag="OO179_001145"
                     /pseudo
     CDS             38023..38871
                     /locus_tag="OO179_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(38909..39970)
                     /locus_tag="OO179_001146"
     CDS             complement(38909..39970)
                     /locus_tag="OO179_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5531289.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40183..41646
                     /gene="bepA"
                     /locus_tag="OO179_001147"
     CDS             40183..41646
                     /gene="bepA"
                     /locus_tag="OO179_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5531290.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41667..42026
                     /gene="arsC"
                     /locus_tag="OO179_001148"
     CDS             41667..42026
                     /gene="arsC"
                     /locus_tag="OO179_001148"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5531291.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42159..42905)
                     /gene="hda"
                     /locus_tag="OO179_001149"
     CDS             complement(42159..42905)
                     /gene="hda"
                     /locus_tag="OO179_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5531292.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(42955..44244)
                     /gene="uraA"
                     /locus_tag="OO179_001150"
     CDS             complement(42955..44244)
                     /gene="uraA"
                     /locus_tag="OO179_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5531293.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44330..44956)
                     /gene="upp"
                     /locus_tag="OO179_001151"
     CDS             complement(44330..44956)
                     /gene="upp"
                     /locus_tag="OO179_001151"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5531294.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45281..46318
                     /gene="purM"
                     /locus_tag="OO179_001152"
     CDS             45281..46318
                     /gene="purM"
                     /locus_tag="OO179_001152"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5531295.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46318..46956
                     /gene="purN"
                     /locus_tag="OO179_001153"
     CDS             46318..46956
                     /gene="purN"
                     /locus_tag="OO179_001153"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5531296.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47128..49194
                     /gene="ppk1"
                     /locus_tag="OO179_001154"
     CDS             47128..49194
                     /gene="ppk1"
                     /locus_tag="OO179_001154"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5531297.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49199..50740
                     /gene="ppx"
                     /locus_tag="OO179_001155"
     CDS             49199..50740
                     /gene="ppx"
                     /locus_tag="OO179_001155"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5531298.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50779..>51887)
                     /gene="pdeF"
                     /locus_tag="OO179_001156"
     CDS             complement(50779..>51887)
                     /gene="pdeF"
                     /locus_tag="OO179_001156"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5531299.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12087 a        13714 c        13486 g        12600 t
ORIGIN      
        1 taataagctt tgctaatata tttcaggaaa accgcttacc ctgaatattc agggtaagcg
       61 tgagagttaa gaatgaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc
      121 atattgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg
      181 cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa
      241 ctaatgcggg cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg
      301 ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca
      361 ccatcaatca tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg
      421 ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc
      481 tcaataccaa aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct
      541 gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc
      601 agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc
      661 acgctaaaat gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca
      721 cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcacg ctgcgcctgt
      781 tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag
      841 cgcggatcgg attcgttatt gacgatttca aaatcaacgc ccgctttgat ctgcaagccc
      901 agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt
      961 cccagcgtta ccagttcctg agtcgcatgc agaacgcgcg cctcttcccc ttccggcaga
     1021 actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt
     1081 ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc
     1141 gccacgcccg actccatcgc cgcttttgcg accgcaggag cgatcttaac gatcaagcgc
     1201 ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg aacgccatac
     1261 gctgaagcca ccacttcact ctgttctgca tgggccagtt ctgcaatcgc gcgtaccgcc
     1321 gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg
     1381 aacgggaagc aaagaacgtt gttcacctgg ttcgggtagt cggaacggcc ggtacagatg
     1441 atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc
     1501 agcgccagga tcattggtgc ccgagccatt ttcttcacca tttcctgggt cagcactttc
     1561 gggccggaac agcccaggaa aatatccgca ccttcaatca catcatcgag ggtacgtttg
     1621 ccgtcatcca ccaccgcata tgcggctttg gtttccgcca tatttggctc acggccctga
     1681 tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagccc cagcgctacc
     1741 agcaggttca tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg
     1801 gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc
     1861 gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca
     1921 atatagaaac attctggcgc tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg
     1981 agcgcggcga cgacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat
     2041 acatcaatcc cagcgaattt cttaaataga acgcccttgc cttccatcac cggtttgcct
     2101 gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat caccgcaacc
     2161 aggttaccgc gcgcggtata tttgtaggct tttagcgggt ctttttcgat ttcaagacaa
     2221 ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg
     2281 gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt
     2341 tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg
     2401 gcaacagccc ctgcccacaa cgtggcgcac attattaccc tgccggagtc tacagacttt
     2461 gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta taacactgat
     2521 gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc
     2581 gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat
     2641 ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagctgcg
     2701 atattgagtc cattcgccat tatcatcccc aggatgccac cactaatcct tcgctgttac
     2761 tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc gcctggggta
     2821 aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca
     2881 atttcggtgc tgaaattctc aaaatcgtac ccggttgcgt gtcaacagaa gttgatgcac
     2941 gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc
     3001 agcaacaagg cgttgagaaa tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa
     3061 ttcgcgcggc cgaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt
     3121 cttttaccca ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag
     3181 ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag
     3241 atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa
     3301 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
     3361 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tctccagtgg
     3421 tacgtaaatt aatcccgcct tctcagacgt tcccacgccc agcacccatg agcgaagcgg
     3481 agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc
     3541 gtctgttcgc tgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
     3601 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
     3661 gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt
     3721 gccgaagtgc tgtggaacga ttttcttaaa cataatccta ccgacccaac ctggtatgat
     3781 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat
     3841 ctgaccggtt acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa
     3901 accccaggcc acccggagat tggctatacg ccaggcgttg aaaccaccac cggcccgctt
     3961 ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag
     4021 tttaaccagc cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc
     4081 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactaggc
     4141 aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac agaaggctgg
     4201 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
     4261 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat
     4321 aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt
     4381 aaggaagagg cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa
     4441 ctgggctggc accatccgcc atttgagatc cctaaagaga tttatcacgc ctgggatgcc
     4501 cgtgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag
     4561 gctcatccgc aactggcaga agagtttccc cgacggatga gcggtggttt accgaaggac
     4621 tgggagaaaa cgactcagaa atatatcaat gagttacagg caaatccggc gaaaatcgct
     4681 acccgtaagg cttcgcaaaa tacgcttaac gcttacgggc cgatgctgcc tgagttgctc
     4741 ggcggttcgg cggatctggc tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg
     4801 aaggaagatc cggcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct
     4861 atcgccaacg gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg
     4921 tttgttgaat acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt
     4981 atggtttata cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt
     5041 gagcaactgg ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag
     5101 atggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg
     5161 atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt
     5221 gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc
     5281 accggttcag agatggaaat taccctgcaa gcggcagaga aattagcagg agaaggtcgc
     5341 aatgtacgcg tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat
     5401 cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc
     5461 gattactggt acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttacggg
     5521 gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg
     5581 gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta
     5641 tgttcccctc accctaaccc tctccccaga agggcgaggg gacggtccgt gcgcttttta
     5701 gggccgcatc tgcgattttg tttaacgggt gatccacaac gtgggcctgg cgtctggccc
     5761 atgccagtta tcgcaggtag gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
     5821 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
     5881 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
     5941 gccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
     6001 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctaa tcatcatcag
     6061 tgctttatca tcggtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
     6121 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttacgtt cttcgagtga
     6181 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
     6241 gctgagaggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
     6301 ctgagcatca ataaacaaca acgccgcttt taagccgctc agagaaatgg tctgattgcc
     6361 gtcgcgtaag gtgatcgcct tcccttcctg aattatctgc aaaaacgcgg tgatggttgc
     6421 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaatgccaa
     6481 cggctcacca tctaatagca gccgtggcgc tatctcccct tcagacgcat ccggcgactt
     6541 caacccaccg cgctcaatac gtaaaacggc atcggtatgc gccccagcgc tgcggctcag
     6601 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acacaaaaat tttgattatt
     6661 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt
     6721 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
     6781 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg
     6841 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgctatttt tcagtccatt
     6901 aaatgtgaca tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
     6961 gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct
     7021 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
     7081 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
     7141 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
     7201 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
     7261 gcgacacgga actgacgaat cagaaccacg ctctttttct tcgcgttgta caggaggatc
     7321 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg
     7381 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
     7441 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
     7501 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
     7561 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
     7621 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
     7681 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
     7741 ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg
     7801 catgacaaat tcactgccct caatcagtac aggtcgccga cgcccctggg catccggctc
     7861 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
     7921 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
     7981 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
     8041 gccgtggcgc agcgcagtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
     8101 gacgttaagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gccccatcac
     8161 ctgtttagtg ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
     8221 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
     8281 ttccaaaagc aaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
     8341 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
     8401 aggaatgccg aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc
     8461 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacctg ccccgataat
     8521 cgccacccgc ttgtccactt tggtgacatg acttaagtca ggacgccaac ctttcgccaa
     8581 cgcctgatct gaaatatagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
     8641 aatagtacag gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg
     8701 cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
     8761 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
     8821 gccgaactta aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata
     8881 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
     8941 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
     9001 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
     9061 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
     9121 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
     9181 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
     9241 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
     9301 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
     9361 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
     9421 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
     9481 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
     9541 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaaccgg caatgttcat
     9601 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
     9661 gactaaattg cgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
     9721 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
     9781 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
     9841 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
     9901 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc
     9961 ggtattaacc aacctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    10021 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    10081 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    10141 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    10201 tttcacgctg gtctttttta ctttgcgccg catacgccat caggtggttg ccccgctgaa
    10261 tcagctggtt accgccagtc agcgtattga acacgggcag ttcgactcgc cgccgctgga
    10321 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga
    10381 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    10441 cgagaccaag cgtcgtctgg aggtgttgta tcagtgcagt gttcccaggc gctgaacacc
    10501 agccagattg atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagca
    10561 gctgaatatc tggagctgaa cgttggggat aactggcgga ttagcgaagg gaagccaaac
    10621 cctgagctgc caatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg
    10681 cactggcaaa atagtcacgt ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg
    10741 ctgggacgcg gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg
    10801 gaagaacgtg cgaccatcgc ccgcgaattg cacgactcgc tggctcaggt gctttcttac
    10861 ttacgtatcc agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa
    10921 agtatcatgg ccgatttttt ccaggcgttg aatgatgctt atcggcagtt acgcgagctg
    10981 ttgactactt tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg
    11041 ctggatacgt tacaaaatca aaccagcgct aaactgaccc tcgactgccg tctgccaacc
    11101 ctggcactgg atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg
    11161 aatgcgatga agcacgccaa cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac
    11221 ggcaatcaca cggtttatat ccgtgataac gggattggta tcggtgaacc gaaagaaccc
    11281 gaaggtcatt atggactgaa tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg
    11341 actttttcac aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctccgcagag
    11401 ggtgagggga aggggaacac gccggctgag gtgtaaataa ttaccctcac accggccctc
    11461 tccctttaaa ggcgagaggg aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc
    11521 aatctcatga taatttacat taactccttt ttttctccac gattggctcg taccttgccg
    11581 ctacagtgaa gcaagtcaag cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg
    11641 gcgaatttct ttattgatcg ccccattttt gcctgggtgc tggcaatcct gttgtgtctg
    11701 acaggtaccc tggcgatttt ttcattgccc gttgaacaat accccgatct cgcgccaccg
    11761 aatgtgcgag tgaccgctaa ctatcccggc gcatcggccc agacgctgga aaacaccgtg
    11821 acccaggtta tcgagcaaaa tatgaccggc ctcgataatc tgatgtatat gtcatctcag
    11881 agcagtggca ccggtcaggc atctgtcact ttaagtttta aagcaggcac cgatccggac
    11941 gaagccgtgc agcaagtaca aaaccagctg caatcagcca tgcgaaagtt accgcaggcg
    12001 gtgcaaaatc agggcgtgac ggtgcgtaaa accggcgata ccaacattct gaccattgcc
    12061 ttcgtctcta ccgatggctc gatggataaa caggatattg ctgattatgt tgccagtaat
    12121 attcaggacc cgttaagccg cgtgaatggc gtcggggata tcgatgccta tggttcgcaa
    12181 tattccatgc gtatctggct ggacccggcg aaactcaaca gtttccagat gacggctaaa
    12241 gatgtcactg atgccattga gtcacagaac gcgcagattg cggttgggca acttggtggt
    12301 acaccttccg tcgataagca ggcgctcaac gccaccatta acgcccagtc actgctgcaa
    12361 acaccagaac agttccgcga tatcaccttg cgggtgaatc aggacggctc agaggtaagg
    12421 ctgggcgatg tcgccaccgt cgaaatgggg gcggagaaat acgattatct tagccgcttc
    12481 aatggtaagc cagcctccgg gttgggggta aaactggcct ccagcgcaaa cgaaatggcg
    12541 acagcggagc tggtgctcaa tcgtctcgac gagctggcgc agtatttccc gcacggactg
    12601 gaatacaagg tggcgtatga aaccacctcg tttgttaaag cctccattga agacgtggtg
    12661 aaaacgctgc tggaagctat cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac
    12721 ttccgcgcca cgctgatacc cactatcgcc gtgccggtgg tgttgatggg aaccttctcc
    12781 gtactttacg ccttcggtta cagcgtcaac accttaacca tgttcgcgat ggtgctggcg
    12841 atcggtctgc tggtggatga cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt
    12901 gaggaaggac tcactcctcg cgaagccaca cgtaaatcga tggggcagat ccagggggca
    12961 ctggtcggga ttgcgatggt gctttcggcg gtatttgtac caatggcctt cttcggcggc
    13021 accaccggtg ccatctatcg ccagttctct attaccattg ttgcggcgat ggtgctgtca
    13081 gtactggtag cgatgctcct cactccggct ctgtgtgcca cgctacttaa gccactgaaa
    13141 aaaggtgagc atcacgggca aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc
    13201 aacgccgaac gctacgaaaa aggggtggcg aaaattctcc accgtagcct gcgctggatt
    13261 gtgatttatg tcctgctgct tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg
    13321 ttcttaccgc tggaagaccg gggcatgttt actacctcgg tacaattgcc cagcggttca
    13381 acccaacaac agaccctgaa agtcgttgag caaatcgaga aatattactt cacccatgaa
    13441 aaagacaaca tcatgtcggt gtttgccacc gttggttctg gccctggggg taacgggcaa
    13501 aacgtggcga gaatgtttat ccgcctgaaa gactggagcg aacgcgacag taagaccggc
    13561 acctcgtttg ccattatcga gcgtgcaacg aaggcgttta accaaattaa agaagctcgc
    13621 gttatcgcca gcagcccacc agcaattagc ggtcttggta gttctgcagg ttttgatatg
    13681 gagttgcagg accacgctgg agcgggtcac gatgcgctga tggcagcacg taatcagttg
    13741 ctggcgctgg cggcggaaaa cccggagcta acccgtgtgc gccataacgg cctcgatgac
    13801 agtccgcagt tgcagattga tatcgaccag cgtaaagctc aggcgctggg cgttgatatc
    13861 gacgatatta acgacacact gcaaaccgcc tggggttcga gctatgtgaa tgactttatg
    13921 gatcgcggtc gcgtgaagaa agtctatgtg caggcagctg cgccgtatcg catgctgcca
    13981 gatgacatca atctctggta tgtccgaaat aaagatggcg gcatggtgcc cttctctgct
    14041 ttcgcgacct cacgctggga aacaggctcg ccgcgtctgg aacgctataa cggttattct
    14101 gcggttgaga ttgttgggga agccgcaccg ggggtcagta ccggtacggc gatggatatt
    14161 atggaatcgt tagtgaagca gctgccaaac ggctttggtc tggagtggac ggcgatgtcg
    14221 tatcaggagc ggctttccgg cgcgcaggct ccggcgctgt acgccatttc cttgctggtg
    14281 gtattcctgt gtctggcggc attgtatgaa agctggtcgg taccgttctc ggtaatgctg
    14341 gtcgtgccgc tgggggtaat cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac
    14401 gacgtttact tccaggtggg cctgttaacg gtcattggtt tatcggcgaa aaacgccatc
    14461 ctgatcgtcg agtttgctaa cgagatgaac caaaaaggcc atgacctgtt tgaagcgacg
    14521 ctccacgcct gccgtcagcg tttacgcccg attctgatga cctcgctggc atttatcttc
    14581 ggcgtattgc caatggcaac cagcacgggt gccggttccg gtggtcagca tgcggtgggt
    14641 actggcgtaa tgggcgggat gatttcagct actattctgg ctatttactt cgtgccgctg
    14701 ttctttgtgc tggtgcgccg ccgcttcccg ctgaagccgc gcccggaata agcaataaaa
    14761 aaggcgacat gccaatgtgt cgcctttttc aactttccga ttaagaacct gctcagcggg
    14821 ttcttgctgt ttatactttg tctcaggaat tacttacgaa gcataacttc gataaagtct
    14881 ttccagttcc ccagttcacg ttcaatcata acaacctctc ttataattat gggtattcta
    14941 cggaaacaat ataccgtggt gaagctaatt tactcgattg ctgcgatgac tacctccggg
    15001 ggacaaacct tatgtaaata ctatggtcct gcggtgataa tttgtatgtg atacacagca
    15061 acatttcgag atattcatac ggcatctaat actgatttaa ttctggttaa aatacagaca
    15121 gataacaaga tgaatattct taatgtttac gttaaaaatg tttaatatta tttaatagtt
    15181 gttaatttga atacttcgat aatgttatat tttctgataa tcatttgcag gcaaaatgtt
    15241 ttcaccctta aatgagtatt tattctcata aatcgaaaaa ggattcatta tggttacact
    15301 ttacggcatc aaaaattgtg acaccattaa aaaggctcgc cgttggctgg aagccaataa
    15361 catcgactat cgttttcatg attaccgcgt cgatgggctg gacagcgaat tattgaacgg
    15421 ttttatcaac gaattaggct gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa
    15481 actggacgaa actacccgca ataaaatcac cgatgcggcc tctgcggcgg cattaatgac
    15541 tgaaatgcct gcaattatca aacgtccatt gctctgcgcg cctggtaagc ctatgctgct
    15601 gggtttcagt gattccagtt atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc
    15661 ggttattgag ctgacacaac agcttattcg ccgcccttcc ctgagtcctg atgacgcagg
    15721 atgtcaggct ttgttgattg aacgtttgca ggcgatcggt tttaccgttg aacgcatgga
    15781 ttttgccgat acgcagaatt tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc
    15841 cgggcatacc gacgtggtgc cgcctggcga cgccgatcgt tggatcaatc cgccgtttga
    15901 acccaccatt cgtgacggca tgttattcgg gcgcggtgcg gcagatatga aaggctcgct
    15961 ggcggcgatg gtggtagcag cagaacgttt tgtcgcacaa catcccaacc atacggggcg
    16021 actggcattt ctgatcactt ctgatgaaga agccagtgcc cacaacggta cggtaaaagt
    16081 cgtcgaggcg ttaatggcac gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc
    16141 gagtatcgaa gtggtaggtg atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa
    16201 cctcaccatt catggcgttc aggggcatgt tgcctaccca catctggctg acaatccggt
    16261 acatcgcgca gcacctttcc ttaatgaatt agtggctatt gagtgggatc agggcaatga
    16321 attcttcccg gcgaccagta tgcagattgc caatattcag gcgggaacgg gcagtaacaa
    16381 tgttattccg ggtgaactgt ttgtgcagtt taacttccgc ttcagcaccg aactgactga
    16441 tgagatgatc aaagcgcagg tacttgccct gcttgaaaaa catcaactgc gctatacggt
    16501 ggattggtgg ctttccgggc agccattttt gaccgcgcgc ggtaaactgg tggatgcggt
    16561 cgttaacgcg gttgagcact ataatgaaat taaaccgcag ctactgacca caggcggaac
    16621 gtccgacggg cgctttattg ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa
    16681 tgccactatt cataaaatta atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg
    16741 tatgtatcaa cgtatcatgg aacagctcgt cgcgtgatga gtggttctgc aagaggaaat
    16801 aagcatggac tggctggcta aatattggtg gattctggtg attgtctttt tggtaggcgt
    16861 cctgctgaac gtgattaaag atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa
    16921 gccggagctt cccccgcatc gtgatttcaa cgataagtgg gacgatgacg acgactggcc
    16981 gaaaaaggat caaccgaaga agtaagctga gtgctcaacc gtaggccgga taaggcgtta
    17041 acgccgcatc cggcaatggt gaacgatccc tgatgcgacg ctcacgcgtc ttatcaggcc
    17101 aggtaatctg tcttttacat catttcaatc acatcatcat cgccaggctt accgccactt
    17161 agcgcttggt cgaaataatg tttcggaatg gtataacgca agtgatccag ggcaaattgc
    17221 atactgcgat tgtcgatcgc atggcctaaa tcctccacga tatccagcgt cacgtcacca
    17281 ccggcactga ttaacgcttc ctgcgccgca actgcatgcg ccagatcgat aaccggatct
    17341 tcaccaccat gaatcaagtg aattgtcgtg gctgtagacg cggtctccgg caggctcgca
    17401 tagcgtccgt taagggcaat gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc
    17461 agcgccataa tcgcgccttg tgagaaacca attagcgccg tggcgttagc ccctactccg
    17521 ctctgtttct gccagtagcg taccgtttca ataaacgtcg gcatgatggc atccacacgc
    17581 gcctggcgat tatcttccgt aataccctgc actgaaaacc actgacgccc cgccggatta
    17641 ccgctcggct ccgcgccacc gacactcacc accagcgcat caggaaacag cggtgcaaac
    17701 cagctgccta tttcccccat tgccaccggg ttatccccga caccgtggaa aagcagtaac
    17761 aactgttgtg caggtttatc cgggctttga acaacaaaat ggtcatgttt catggcgaac
    17821 tccttaactg atgtcattaa ttttacgccg ctgactcaca atgaccatgg gagaaaattg
    17881 aaggagtcag tgaaaaaatt gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac
    17941 ctcatttagt gcgaacagcg cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt
    18001 gcggcctgaa agtttaagtg tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg
    18061 cccacgcagc gccggtaatg ccagttcact ggtttgtagc agacgcaata agcaacctaa
    18121 cgatgttaat agcggacgat gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc
    18181 agaaaggatg gcatcgttta gtggatcaac aggaagcatt tcgccattcc actgcgcgag
    18241 agcttgcgca tcgcgacgta aacggtaatg ctcacgttca gccagctgtt tacccgcatc
    18301 actcatcggt aacagcgcca tcgccgtata gcaaccgctg ctggcttccc gatgattacc
    18361 catccgcacc agcacaaaac cgcagcgttg ccagaaacgc cataactccc cagtgtaacc
    18421 aaaactcacc gaaagatagt cgaggtcatg cgtatattgc aaagctccag caataagttg
    18481 tcgccctgtg ccttcccgct gacgagccgg atgaactgct atccggctaa cccgccttcc
    18541 gcgcaatgtc gccgccagtg gattgctgcc gtgcgccgcc agcgactggg ccaccagatt
    18601 accccgcggg cgacgaaaac ctgcccatac cgcctgactg agttcttgag ataatccgcc
    18661 ctcatccacc agccacaacg ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg
    18721 ttgccctggt gcatccatca tccggcgtaa atccagcggc gaggtccggt agtgcgcacc
    18781 agacaataac tgataaacct ttaacggcgt ttctggctcg cttcgccata acgtttgttc
    18841 aaatgcggaa atgacgatat tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac
    18901 cagtgcctca ctaaccattt tttccagcgg gcatccctgt gcccagcgga tcggctgttg
    18961 cagttcaaaa cggtgtaaat gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc
    19021 ggtaccttcg tagccctgca ccgtagtggt taacaacgtt cgaggaaaac gcgataccag
    19081 ttgatgcaac aacggcgcag gtatggctgc ggcttcgtcg accaccagcc agtcggcttg
    19141 ctcatcgctg gctaacaagg catccggtgc aataaagcga aacttctcgc ccgcaaattg
    19201 tgccagtaca tccgttgccg cttttgcggg cgcggtgaca atcgcactgc ccgcaatacg
    19261 agaaatgagt tgccctgcca acgccgactt accgcgccca cgcgcagccg ttaccgctgc
    19321 cacgcccggc ggcatggtca gtagctgctg taagagttgc tgttgttctg gttgtggtgc
    19381 gccggtcgcg gggtgccagt cagtacgggg agtaaaatgc gccaacgaga acggctggtt
    19441 ttgccgccag aggatagcgt cgttatccgc cgtaagtacg cgtttgagat gctggacaaa
    19501 atgcggcgtc gcaatagggt cagggcaatc actccagcgc agcgagtcgg catcaggttg
    19561 gttttcccac tcttcccata cagggagtaa caaaaccagc cagcttcccg ctttcaacgt
    19621 tccgctaagt gctgcaaagg cagcggcatc aaagccgtgg cgggcgtcga ataccgcatg
    19681 ccggaactcg cgcccaagta aagtttgtag cgccgagggt gaacagtggt tttcagcatc
    19741 tagctgcggc gaaatccaca gccagtcgcc aggtaaggca tcacgcaact taagagcatg
    19801 atcaaaacac caaccctctt ccccgctcaa caccagcaag cggcagatcc cttcacgttt
    19861 catttgcgct gttaatgtgt aaagcgcagt cagttcagcc atccctgccc cggaaattaa
    19921 atgcttttac caaaagtatt gcattgtgcc ggatcgccgc tgtcgaaacc acgtttaaac
    19981 cagctgtagc gttgctgaga agtgccatgt gtgaaactgt ctggtactac tcgcccctga
    20041 ctttgctgtt gtaaacggtc atcgccgatg gcctgcgccg cattcagcgc ctcttccaga
    20101 tcgccagttt ccagaacgcc ttgctgctgc atactatgcc cccagacacc ggcaaaacag
    20161 tcggcctgga gttccatacg cacagataag cggtttactt ccgcctgcgt cgcgttttgt
    20221 tgcagttgac gaactttcgg ctcgatgcct aacagtttct gcacatgatg accgacttca
    20281 tgggcgataa cgtacccctg ggcaaaatcg ccatccgcgc caagtttgtc tttcatgtca
    20341 tcatagaagg agagatcgat ataaactgtg ccatccgccg ggcaatagaa cggccccata
    20401 atggactggc ccgccccgca gccggtgcgc gtcattccac ggtacatgac cagcttcggt
    20461 tgctgatagg tcttacccat cttttcgaac tgttgtcccc aggtgtcttc cgtggttgcc
    20521 agaatcaccg aggtgaattt tgcggcttcg tcttcattag ggctaattga ccgcgttgat
    20581 tgttgttgag aaaccggctg cccggtcatc aacccggtta aatcaacgcc atagtagcct
    20641 gcaaccagca cgactatcag taaaatcagc ccgcctttac cgctcggcag acggaaaccg
    20701 ggaccgccca ttgacggacc gccagagctg ttgcgcctgt cttcaacatt gtcactttca
    20761 cgtcgccctt gccaacgcat agatacctca acaatatatt cattatagaa atgatcgtag
    20821 gtggttcaga ggaagattac cacaggaaag cgggatgaaa tggcggagga atgagcgttc
    20881 aatgcatata ttccgagagc ggttgtcttg ccgtgccagc tgcacggcaa gatgatgaac
    20941 agaaaaatca gtccagctgt acacccaggc ggcgggcgac ggcttcatag gcttcgatca
    21001 ggccaccgag gctctggcgg aaacggtctt tgtccatttt ctccagtgtt tctttgtccc
    21061 acaggcggct gccgtccggg gagaactcat cgcccagtac cacttcacct ttgtacagac
    21121 caaactccag cttgaagtcg accagaatca gaccagcatc atcgaacagt tttttcagca
    21181 cgtcgttcgc tttgtaggtc agctctttca tacgcgccag gttctctttg ctcacccagc
    21241 caaaggtttc gcagtaagat tcgttgacca tcggatcgtg catggcatcg tttttcagga
    21301 acagatcgaa cagcggcggg ttcagctcaa taccttcttc gattccaaga cgtttcacca
    21361 gagagccagc agcacggtta cgcacgacac actcaaccgg caccatatcc agctttttca
    21421 ccagacattc ggtatcggag agcagacgct ccatttgagt cgggataccc gcttcagcca
    21481 gtttgctcat aatgaagtag ttgaacttgt tgttcaccat acctttgcga tcaaactgct
    21541 caatgcgcgc gccatcccct gctgacgtat cattgcggaa ttcgagcacc aacaggtccg
    21601 ggttttccgt gctgtatacg gttttcgctt taccacgata caactcagct tgcttttgca
    21661 tctttatcac tcctgggtgt gaattaacgt tttaaaatct tttgctgtct ggtgtgccgg
    21721 atgttttgtc ggatgcggcg tgaacgcctt atccgaacta cgtccgacaa acaaatttcg
    21781 tgcgaattac cgctaaaatc gcgttttcct gtcgacgcac acgtttgcgt atcatatcag
    21841 aaaaaagggc cggatgattc cagccctgta tttttacttg ctaaacgcag cctggaagac
    21901 agctaccagc gcgtcgttct gactctgagt cagagtatga cctttcggat cgatgaactg
    21961 taggctgctg cggttatcta aatcgccaac ctgcagttta tagtcaccgg atgccaggcc
    22021 tggatcgctc gcgcccagtt cctgccagtc gctgtcagac agcggcttat aagttacggc
    22081 catgttgccc tgcgaacggg tgctgtcggt cactttcatg cccacttttt ccagcgccgc
    22141 tggcagacgt tgccaaacca cattgaacgg cccgcgtacg accagcattg gtaaaccggt
    22201 gtcatcagct gcactttgta cgtccatagt ggtggaggca cgattttgcg cagcgttcgc
    22261 ggcgtcagtg gcagatttat ccagaccggc ggaaataacg ttcatcatct ccgtgctgta
    22321 acgctgcatg gaagccgcgt ctgcaaccgg tttgcccgcc tgttccaggt tcagcagttt
    22381 aaccgtaacc gcctgctgat aaccctgcgg cttaacagag atttgataac gaccacgata
    22441 ctgctcgtct tcgtccagac ggttccattg tacccaatcg gtggtcagtg tctgaccagc
    22501 atcatcacgt tgggtgatgg tgtagttttt cgcctgcagc acgctaacca cctgcggcca
    22561 cagagtattg ccacgaccat tttccaccag caatgaagcg gtatcgcccg tgaactgggt
    22621 acgcgcgcca gaaaccagtg ccaacggctg ggctggtgga cgaatgtcca gcgccttacc
    22681 gacagcacca ctaccgttgg tcaccgggat tgcataatca ccggaggtca ccggcaaaat
    22741 cattccagcc ggggcatgaa gctccgcaag cggtgccgct tccaggtagg cttcatcacc
    22801 actgacctga cgcttatagc gtgagtcaga actacaggca gcgagtaata aaacaagcga
    22861 aacacccgca acctttgcca ggcgcgactt ttgaacagag taagccatca aatctcccta
    22921 aactttacag caaaccggca tgcttaagcg ccgctctgac cgtctcacga ccactgtcgg
    22981 tgattggtgt cattggcagg cgcagcgtat cggtcgccac aagacccagt tccttacatg
    23041 cccatttcac cgggattgga ttgggttcga caaatagttt gttgtgtaat ggcatcagac
    23101 gctgattaat aacgcgtgcc tcggcaaaat gcccttctgc tgccagtttg cacatctggg
    23161 ccatatcacg cgctgcgacg ttagccgtaa cggaaataac tccatgaccg cccaattgca
    23221 tgaagtccag cgcgctcgca tcatcgccgc tcagcagaac aaaatcatct gaaaccagct
    23281 ctttgatctg gtttacacgc gttaagttcc ctgttgcctc tttgattccg ataatatttt
    23341 ttactttcgc cagacggccc accgtttccg ggagcagatc gcagccagta cgggacggca
    23401 cattatacag aatttgcggc aggtcagtat gctcagcgat ggctttgaaa tgctgataca
    23461 aaccttcttg cgacggacga ttgtagtaag gggttaccgt caggcagccg acgataccac
    23521 tgtcattgaa gcgctgcgtc aggctaatgg cttccgcagt agcgttagcg ccggtcccgg
    23581 caattaccgg aatgcgccca tcagccagat ccagcgtcat catcaccaca tcagcatgtt
    23641 cgtcatgatt taaggtagcg gactcgccag tggtgccaac agaaacgatc gccgaagtac
    23701 cgctggcgac atgataatca atcagttttt tcaagctagc ccgacagaca ttaccttttt
    23761 catccatcgg agtaacaatc gcgacaatac ttcccgtgaa catgggccat cctctgtgca
    23821 aacaagtgtc tcaatggtac gtttggtatg gcattaaaag caagcagaca gaaccgttct
    23881 gattgttgta tgcatgattt ttttatgctt tccttaagaa caactcaccc cttaaaggaa
    23941 taaccagttt gacactgtca tcgcaacatt atctggtgat cactgcgttg ggtgccgatc
    24001 gccctggaat tgtgaacacc atcacccgtc atgtcagtag ttgcggctgt aatattgaag
    24061 acagtcgcct ggcgatgctg ggagaagagt tcacgtttat catgctgctt tccggttcat
    24121 ggaatgccat cactctgatt gaatcaacgt taccgttgaa aggtgccgaa ctggatcttt
    24181 taatcgtgat gaagcgcacg acggcgcgtc cgcgtccgcc aatgccagca tctgtctggg
    24241 ttcaggtcga tgttgcagac tccccgcatt taattgaacg cttcacagca cttttcgacg
    24301 cgcatcatat gaacattgcg gagctggtgt cgcgcacaca acctgctgaa aatgaacggg
    24361 ctgcgcagtt gcatattcag ataaccgccc acagccccgc atccgcggac gcagcaaata
    24421 ttgagcaaac gttcaaagcc ctatgtacag aactcaatgc acaaggcagt attaacgtcg
    24481 tcaattattc ccaacatgat gaacaggatg gagttaagta atgaatccac tgaaagccgg
    24541 tgatatcgca ccgaaattta gcttgccgga tcaagacgga gaacaagtta atttgaccga
    24601 cttccaggga cagcgtgttc tggtttattt ctacccgaaa gccatgaccc ccggctgtac
    24661 cgtacaggcc tgcggcttac gcgataacat ggatgagttg aaaaaagcgg gcgttgatgt
    24721 gctgggtatc agcaccgata aacccgaaaa actctcccgt tttgcggaaa aagagctgct
    24781 taactttacg ctcctgtctg atgaggacca ccaggtgtgc gaacaattcg gcgtctgggg
    24841 tgaaaagtcc ttcatgggca aaacctacga tggtattcat cgcatcagct tcctgattga
    24901 cgctgatggc aaaatcgaac atgtctttga cgatttcaaa accagcaatc accacgacgt
    24961 tgtgctgaac tggctgaaag aacacgcctg attactttgc tccattccgt gctggctgcg
    25021 cttgcggcca gcatacctca cttctcgtga tcaagatcac attctcgctt tcccctgcga
    25081 cacgggtgtc gaatccattt tttgctgaac gttaatgacc atcatttttg taccgttcag
    25141 aatccagtta atacataact tattgaatag attgagttaa tcagaatggc atcctttata
    25201 caatatgaaa tgcaatgttt catatcattt tcaaggagcc gacatgaacc gctttgtggt
    25261 ggccgaacca ctgtggtgta caggatgtaa tacctgtctc gctgcctgtt cggacgtgca
    25321 taaaacgcaa ggtttacagc aacacccgcg cctggccctg gcgaagacgt caacaatcac
    25381 tgcccctgtc gtgtgtcatc actgtgagga agccccttgc ctgcaggtct gcccggtcaa
    25441 tgccatctct cagagggatg atgcgatcca actcaacgaa agcctctgta ttggctgcaa
    25501 gctttgcgcc gtggtctgcc catttggcgc aatcagcgct tcaggaagcc gtccggtgaa
    25561 tgcccatgcg caatatgttt ttcaggctga aggctcactc aaagacggcg aagaaaacgt
    25621 gccgccccaa catgcgttgc tgcgctggga accgggtgtc cagaccgtcg cggtgaaatg
    25681 cgacctgtgt gatttcttgc cagaaggtcc ggcctgcgtt cgcgcttgcc cgaatcaggc
    25741 gttacggctg atcaccgacg atagcctgca acgtcagatg aaagaaaaac agcgccttgc
    25801 cgcaagctgg tttgccaatg gcggggagtc tcccctttcc ctcactcagg agcaacacta
    25861 atggatgccc tgcaattatt aacctggtcg ctgatcctct atctgtttgc cagtctggct
    25921 tcgctgtttt tactcggtct ggacagactg gctattaagc tttccggcat cacatcgctg
    25981 gtgggcggcg tgattggcat catcagcgga attacgcaat tacatgctgg tgtaacttta
    26041 gtcgcccatt ttgccacccc ttttgacttt gccgatttaa ccctgcgaat ggatagcctc
    26101 tcggcattta tggtgctagt tatctccttg ctggtggtgg tttgttcgct ctattcattg
    26161 acttatatgc gcgaatacga gggcaaaggc gcggcggcga tgggcttctt tatgaatctt
    26221 ttcatcgcat cgatggttgc cctgctggtg atggacaacg ctttttggtt catcgtgctg
    26281 tttgaaatga tgtcgctgtc ttcctggttt ctggtcattg ccaggcagga taaaacgtcg
    26341 atcaacgctg gcatgctcta cttttttatc gcccacgccg gatcggtgct gattatgatc
    26401 gccttcttgc tgatggggcg cgaaagcggc agcctcgatt ttgccagttt cgcacgcttt
    26461 cactttctcc ggggctggcg tcggcggtgt ttctgctggc ctttttcggt tttggcgcga
    26521 aagccgggat gatgccgttg cacagctggt tgccgcgcgc tcaccctgcc gcaccatcgc
    26581 acgcttcggc gttgatgtct ggcgtaatgg tcaaaatcgg tattttcggc atcctgaaag
    26641 tagcgatgga tctgctggcg caaacgggtt tgcctctgtg gtggggcatt ctggtgatgg
    26701 cgatcggcgc aatctccgcg ctcctgggcg tgctatatgc gctggcggaa caggatatca
    26761 aacggctgct ggcctggagt accgtcgaaa acgtcggcat tattttgctg gcagtcggtg
    26821 tggcgatgga cggtctgtca ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg
    26881 cactgtttca tctgctcaac catgcgctgt tcaaagggct gctatttctc ggcgcggacg
    26941 cgattatttc gcgtttgcat acccacgaca tggaaaaaat gggggcactg gcgaaacgga
    27001 tgccgtggac agccgcagca tgcctgattg gttgcctcgc gatatcagcc attcctccgc
    27061 tgaatggttt tatcagcgaa tggtacacct ggcagtcgct gttctcacta agtcgtgtgg
    27121 aagccgtagc gctacaactt gcgggtccta ttgctatggt aatgctggca gtcactggtg
    27181 ggctggcagt aatgtgcttc gtcaaaatgt acggtattac tttctgtggt gcgccgcgca
    27241 gtacacacgc tgaagaggca caggaagtgc caaatacgat gatcgtcgcc atgctactgc
    27301 tcgcggcact ctgcgtatta attgcgctta gtgccagttg gctggcaccg aagataatgc
    27361 atattgccca tgcgtttacc aatacccctc ccgtcactgt cgccagcgga atagcacttg
    27421 tacccggcac gtttcataca cgggtcactc cttcattact gttgctgtta ctactggcga
    27481 tgcctttgct gccaggcctt tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc
    27541 gcacaggaga tgcctgggca tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca
    27601 atggcgtgat gcagccgctg cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc
    27661 tcgaccctac gctgaggcta aataaaggtc ttgcgcacgt caccgccagg gctcagagca
    27721 cagaaccctt ctgggatgag cgggtgatcc gccccatcgt gagcgccacc caacggctgg
    27781 tcaaagaaat acagcatctg caaagcggcg actttcgtct ctattgcctg tatgtggtcg
    27841 ccgcactggt tgtgctgcta atcgctattg ccgtctaagg aaatcaccat gagacaaact
    27901 ctttgcgacg gatatctggt catttttgcg ttagcacagg ccgtgattct gctgatgcta
    27961 accccacttt ttacgggtat ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg
    28021 ccggggatct ggcaggatta tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg
    28081 ccgacatctt caggtctgat gttccgcctg atgccgtggg tattaatcag cagcatgctg
    28141 gtgctggcga tggccttacc actatttatt accgtttccc cttttgcggg cggcggcgat
    28201 ctgatcaccc ttatctatct tcttgccctg tttcgttttt tctttgctct ttccgggctg
    28261 gataccggaa gtccgtttgc gggagtcggt gccagtcgcg agttgacgct cggcattctg
    28321 gtcgaaccaa tgcttattct ctcactgctg gtattggcgc tgatagcagg ttccacgcat
    28381 atcgagatga tcagcaatac gctggctatg ggctggaact cgccgctaac caccgtactg
    28441 gcgttactgg cctgtggttt tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt
    28501 gctgaagcag aacaggaatt acaggaaggc ccgctgaccg aatattccgg tgccgggctg
    28561 gcgctagcga aatgggggct ggggctgaaa caggtcgtga tggcagcact gtttgtggcc
    28621 ctgtttctgc cctttgggcg cgcgcaagaa ctttctctcg cctgcctgct gacttcactt
    28681 gtcgttacgc tgctcaaggt tttgctgatt tttgtactgg cctcaatcgc agaaaacacg
    28741 ctggcacgcg ggcgtttttt actcattcac catgtgacct ggcttggctt cagccttgct
    28801 gcgcttgcat gggtcttttg gttaaccggt ctgtaaggag cactgacgga atatggaaaa
    28861 tcttgctctg acgacgttaa tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc
    28921 acaacgtcgg gccgccgaat ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt
    28981 gtcgctgatc tccgcgtttt atcaggccga taaagttgcc gtcacgttga cgttggtcaa
    29041 cgtgggggat gtggcattgt ttggcctggt cattgatcgc gtgagtacgc tgattctgtt
    29101 tgtggtggtg tttctcggtt tgctggtcac gatctactcc acgggttatc tgacggataa
    29161 aaatcgcgaa cacccgcata acggcacgaa tcgttattac gcatttttgc tggtgtttat
    29221 cggcgcgatg gcgggactgg tagtctcctc gacgctgctc ggtcagttgt tgttttttga
    29281 aattacgggc ggctgctcct gggcgttgat cagttattac cagagcgata aagcgcagcg
    29341 ttcagcacta aaagcgttgc ttatcaccca tatcggttcg ttggggttgt atcttgccgc
    29401 cgccacgctg tttttgcaga ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg
    29461 cgacgcacgt tatctggttt atggcggcat cctgtttgcc gcgtggggga aatcggccca
    29521 gctaccgatg caagcgtggc taccggacgc aatggaagcg ccaacaccga tcagcgccta
    29581 tctccacgcc gcatcgatgg tgaaagtggg cgtttacatt tttgcccgcg ccattatcga
    29641 cggcggcaat atcccgcatg tgattggcgg cgttggcatg gtcatgacac tggtcaccat
    29701 tctttatggc tttctgatgt atttgccaca gcaggatatg aagcggttgc tggcctggtc
    29761 gaccatcact caacttggct ggatgttctt cggcttgtcg ctctccatct tcggctcgcg
    29821 gctggcgctg gagggtagca tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt
    29881 tttccttgta gcaggtgcgc tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg
    29941 tggcgtattg cacaccctgc cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat
    30001 taccggtgtg ccgccgttca acggcttctt cagtaaattc ccgctgtttg ccgccggttt
    30061 tgcgttgtca gtggagtact ggatcctgct gcccgccatg attctgctga tgattgaatc
    30121 ggtcgccagt ttcgcctggt ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag
    30181 cgaggccgtc gccgatgccg caccgctgcc agggtcaatg cgcctggtgt tgattgtact
    30241 gattgtgatg tcgctgattt ccagcgtaat cgccgcgacc tggttgcagt aaggagatga
    30301 tgaatgaccg gttctatgat cgtaaataat ctggcgggac tgatgatgct gacatcgctg
    30361 tttgtgatta gcgtcaaaag ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg
    30421 gtgctggtgt ctattttcgc cactctctcg tgcctgttcg ccgcagagca actgctgatc
    30481 tggtccgcca gcgcctttat caccaaagtg ctgctggtac cgttaatcat gacttacgct
    30541 gcacgaaata ttccccagaa catcccggaa aaagcgttat tcggtccggc aatgatggca
    30601 ctgctcgcgg cgttaattgt cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg
    30661 atggctaccg ggctgaaacc ggcgctggcg gtagcgttag gtcattttct gcttggcctg
    30721 ctgtgcattg tcagccaacg caatatcctg cggcaaattt ttggttactg cctgatggaa
    30781 aacggctccc atctggtgct ggcgcttctt gcctggcgag caccggaact ggtggaaata
    30841 ggtatcgcta ccgacgccat cttcgccgtc attgtgatgg tgttactggc aagaaaaata
    30901 tggcgtaccc acggcacgct ggacgtgaac aacttgaccg cgctgaaggg ataatgagat
    30961 gagttattct gtgatgttcg ctttactcct gctcacgccg ctgctttttt cgctgctctg
    31021 ttttgcctgc cggaaacggg ggctctctgc gacttgtacg gtgactgtat tacatagctt
    31081 agggatcaca ctgctgctga ttctggcact ctgggtggtc caaactgccg ctgatgcagg
    31141 agaaatattc gctgcgggac tgtggcttca tattgatggt ctgggcggtt tgttcctcgc
    31201 cattcttggt gtgattggct ttctcaccgg tgtttactcg attggctaca tgcgtcatga
    31261 agtggcacac ggcgagcttt cacccgttac actgtgcgat tactacggtt tcttccatct
    31321 gtttttgttc accatgctgc tggttgttac cagcaataac ctgattgtga tgtgggcggc
    31381 gatcgaagcc accaccttaa gctcggcgtt tctggtaggc atttacggtc agcgttcatc
    31441 gctggaatca gcatggaagt acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt
    31501 cggtaccgtg ctggtatacg ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat
    31561 attctggagc gaggttctta agcaatcgtc cttgcttgac ccaacattaa tgctgttggc
    31621 ctttgtgttt gtgctaattg gctttggtac caaaaccggg ctatttccca tgcacgcctg
    31681 gctgccggat gctcacagtg aagcgccgag tccggtcagc gccctgctct ccgccgtatt
    31741 gctgaactgc gcgctgttgg tgctgattcg ctattacatc attatttgcc aggccatcgg
    31801 cagcgatttc cccaaccggt tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc
    31861 atttttcatt ctggtacagc gggacattaa gcgtctgctg gcgtactcca gcgtggagaa
    31921 catggggctg gtcgcggtgg cgctaggcat tggcgggccg ctgggaattt ttgccgcgct
    31981 gctgcacacc ttaaaccaca gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt
    32041 actgctcaag tacggcacgc gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc
    32101 atttaccgcc gtgctgtttg gcggcggtgc gctggcgctg gcagggatgc cgcccttcaa
    32161 catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat
    32221 tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac ggatggctgc
    32281 gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac
    32341 cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc atattccaca
    32401 acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct
    32461 gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga
    32521 gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga
    32581 aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca
    32641 ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg
    32701 gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt
    32761 attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg
    32821 atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg
    32881 agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc
    32941 tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt
    33001 atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca
    33061 acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc
    33121 acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg
    33181 tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct
    33241 tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg
    33301 ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc
    33361 tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg
    33421 gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg
    33481 aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg
    33541 atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgcgccgacg
    33601 tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca
    33661 ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg
    33721 cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc
    33781 aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa
    33841 agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc
    33901 actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc
    33961 gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg
    34021 gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa
    34081 taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga
    34141 ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat
    34201 cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa
    34261 gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct tcgcgccact
    34321 ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg
    34381 cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca
    34441 aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga
    34501 agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg tcacccataa
    34561 agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc
    34621 gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc
    34681 tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg
    34741 cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt
    34801 ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg
    34861 aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc
    34921 ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa
    34981 cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc
    35041 acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat
    35101 aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac
    35161 agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg
    35221 ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag
    35281 attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag
    35341 atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt
    35401 caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat
    35461 cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa
    35521 ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac
    35581 gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat
    35641 gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt
    35701 ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg
    35761 acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc
    35821 cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg
    35881 tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat
    35941 ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca
    36001 gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc
    36061 gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg
    36121 ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat
    36181 taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg
    36241 gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga gctacgcttt
    36301 attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa ctggcaggca
    36361 cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca ggtggcgctg
    36421 cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg
    36481 ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt
    36541 ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc
    36601 ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc
    36661 gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca
    36721 gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc
    36781 gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg
    36841 gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc
    36901 gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag
    36961 gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc
    37021 ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg
    37081 gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc
    37141 gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc
    37201 gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt
    37261 actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt
    37321 ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag
    37381 gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt
    37441 gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc
    37501 ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat
    37561 atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt
    37621 gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg
    37681 cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat
    37741 ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt
    37801 cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga agaagaacgt
    37861 cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg
    37921 gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc
    37981 tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct
    38041 ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa
    38101 attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg
    38161 atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata ggcattaacc
    38221 catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt ttgcggcacc
    38281 agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt tattagttgg
    38341 cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg tattgcctgt
    38401 ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat gtggggagtc
    38461 gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc tgtcagcctc
    38521 ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg cggacgttcg
    38581 ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc cagtggcttt
    38641 gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg ccatttcgcc
    38701 cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggtc
    38761 agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca
    38821 gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc
    38881 ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg
    38941 cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc
    39001 cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg
    39061 cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca
    39121 ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc acgccaacca
    39181 ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca
    39241 ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag gtggcgatcc
    39301 ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct
    39361 ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga acggcgttca
    39421 gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga
    39481 cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca
    39541 tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta
    39601 acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg
    39661 gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca
    39721 tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc
    39781 agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc acaatagcca
    39841 ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa
    39901 taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca
    39961 tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc
    40021 gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa aaagtcattt
    40081 gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga tatttcacag
    40141 tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa
    40201 aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt
    40261 gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt
    40321 ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta
    40381 attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat
    40441 gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc
    40501 tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac
    40561 gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg
    40621 gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt
    40681 tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg
    40741 gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc
    40801 attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc gaccttcctg
    40861 gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac
    40921 ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg
    40981 gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat
    41041 tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt
    41101 cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac
    41161 gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc
    41221 gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg
    41281 aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc gaacgcttat
    41341 ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat
    41401 aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct aaataaccgc
    41461 gatcaggagc tggctgcgcg agcagaaggt tatgcactcg ccggacgact cgatcaggcc
    41521 atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca acaagcgcgt
    41581 tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag
    41641 atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac
    41701 gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag
    41761 tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc
    41821 tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactaa
    41881 acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccaa
    41941 agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt cgtccaccgg
    42001 agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg
    42061 gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac ttcgcgtagg
    42121 tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
    42181 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
    42241 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
    42301 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
    42361 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc gagatccggt
    42421 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg tgttttgccc
    42481 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
    42541 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
    42601 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
    42661 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
    42721 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag taaagaggag
    42781 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
    42841 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
    42901 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
    42961 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactgat
    43021 cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca taccttttaa
    43081 ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt
    43141 caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc
    43201 ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
    43261 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
    43321 cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
    43381 gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca ggccgttagc
    43441 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
    43501 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
    43561 aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc agtgagcatt
    43621 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
    43681 gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac ctaaaaccgt
    43741 gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt gcccttcagc
    43801 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
    43861 gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc ccgctttctt
    43921 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
    43981 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
    44041 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
    44101 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
    44161 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
    44221 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
    44281 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
    44341 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
    44401 cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
    44461 taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
    44521 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
    44581 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
    44641 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt tccagcacac
    44701 cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc ttacctttga
    44761 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
    44821 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
    44881 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
    44941 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
    45001 tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca aaatgataac
    45061 agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa
    45121 aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa aagacgtgca
    45181 aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat
    45241 tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct
    45301 tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa
    45361 aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
    45421 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt
    45481 aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct
    45541 ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga
    45601 ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca gcggcatcgc
    45661 ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag aaatgccggg
    45721 gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc
    45781 agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
    45841 cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga
    45901 tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg
    45961 catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca
    46021 cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata atacccaggc
    46081 agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggcagg
    46141 taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc
    46201 cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc
    46261 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg
    46321 aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt
    46381 aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc cgacgcgttc
    46441 ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt
    46501 gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat
    46561 gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc
    46621 gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat
    46681 cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt cgtcaccgat
    46741 gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg
    46801 gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt
    46861 agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa
    46921 cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta aagcgccagc
    46981 tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt ttttttcccc
    47041 cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca ggcagtgtcc
    47101 cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat cgaaaaagag
    47161 ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa atctaacccg
    47221 ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga gttctataaa
    47281 gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg ctccaacgct
    47341 cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga tcaggaattc
    47401 gacggcctct acaacgagct attgctggag atggcgcgca accagatctt cctgattaat
    47461 gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa gcagtatctg
    47521 cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca gttcctgaaa
    47581 gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg ttacgcgctg
    47641 ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt
    47701 cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga tgatattttc
    47761 aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc
    47821 gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc ttccagtctc
    47881 aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg
    47941 ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat cgtccccggc
    48001 ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa agccaatctg
    48061 gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca gttccgcaat
    48121 ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca cacctttgag
    48181 catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc gattaaaatt
    48241 aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca cgccgcacat
    48301 aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga agaagccaac
    48361 attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg
    48421 ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga agtggtccgt
    48481 tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta tactgactat
    48541 tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt taactttatt
    48601 gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca aaactctcgc
    48661 cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt
    48721 ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg tctgtatgcg
    48781 gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc
    48841 aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg ttaccttgaa
    48901 catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc
    48961 gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct gctcgatccg
    49021 cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac ggtcaaagca
    49081 cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta
    49141 cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga ataaccctat
    49201 gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa
    49261 cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta ttggccgcct
    49321 gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga gtgaagaggc
    49381 aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag ggttttctcc
    49441 tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg ccactgactt
    49501 tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg gtaatgaaga
    49561 agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt
    49621 tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg aacctattct
    49681 cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc ctggcggggt
    49741 catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac tggaaacttt
    49801 aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg gtaccataaa
    49861 agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta ccccggaacg
    49921 tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc
    49981 gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt tatgtggtgt
    50041 gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt
    50101 actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag
    50161 cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg ataccactat
    50221 gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc aactggaggc
    50281 gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc acagcggttt
    50341 gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca
    50401 gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc tcgacgatct
    50461 gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc tattgcgcct
    50521 tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat tgacactgat
    50581 tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc agaatgcgct
    50641 ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg gctggcggtt
    50701 gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga
    50761 aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat tcagcgtatc
    50821 aattaacggt tgcggcttac caataagata accctgcata taatcgatcc ccaaagagag
    50881 caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca ttttcttcat
    50941 tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg acacaatatt
    51001 gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa gccgtgcata
    51061 gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt cctgaagatg
    51121 ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg tgacttcaaa
    51181 aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt caacaggaaa
    51241 acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag cgggcatctt
    51301 cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt cgatactcga
    51361 cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca gttcatcatt
    51421 ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac cggtaatcgg
    51481 ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat tcatcatcgc
    51541 gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca tattttccgg
    51601 ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca gcaggtagat
    51661 atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt gcatcggcat
    51721 cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa tgcgctcctg
    51781 gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat aaacatcttc
    51841 acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgt
//
LOCUS       DAJTNW010000019        49549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNW010000019 DAJTNW010000000
VERSION     DAJTNW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..1168)
                     /gene="setB"
                     /locus_tag="OO179_001157"
     CDS             complement(<1..1168)
                     /gene="setB"
                     /locus_tag="OO179_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5531300.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMT"
     gene            1536..2666
                     /gene="fruB"
                     /locus_tag="OO179_001158"
     CDS             1536..2666
                     /gene="fruB"
                     /locus_tag="OO179_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5531301.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2666..3604
                     /gene="fruK"
                     /locus_tag="OO179_001159"
     CDS             2666..3604
                     /gene="fruK"
                     /locus_tag="OO179_001159"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5531302.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3621..5312
                     /gene="fruA"
                     /locus_tag="OO179_001160"
     CDS             3621..5312
                     /gene="fruA"
                     /locus_tag="OO179_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5531303.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5736..6677
                     /locus_tag="OO179_001161"
     CDS             5736..6677
                     /locus_tag="OO179_001161"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5531304.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6665..7603
                     /gene="psuG"
                     /locus_tag="OO179_001162"
     CDS             6665..7603
                     /gene="psuG"
                     /locus_tag="OO179_001162"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5531305.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7697..8947
                     /locus_tag="OO179_001163"
     CDS             7697..8947
                     /locus_tag="OO179_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5531306.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9018..9712)
                     /gene="yeiL"
                     /locus_tag="OO179_001164"
                     /pseudo
     CDS             complement(9018..9712)
                     /gene="yeiL"
                     /locus_tag="OO179_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9843..10784
                     /gene="rihB"
                     /locus_tag="OO179_001165"
                     /pseudo
     CDS             9843..10784
                     /gene="rihB"
                     /locus_tag="OO179_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10884..12134
                     /locus_tag="OO179_001166"
     CDS             10884..12134
                     /locus_tag="OO179_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5531307.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12190..13278)
                     /locus_tag="OO179_001167"
     CDS             complement(12190..13278)
                     /locus_tag="OO179_001167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5531308.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13281..14138)
                     /gene="nfo"
                     /locus_tag="OO179_001168"
     CDS             complement(13281..14138)
                     /gene="nfo"
                     /locus_tag="OO179_001168"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5531309.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14212..15261)
                     /locus_tag="OO179_001169"
     CDS             complement(14212..15261)
                     /locus_tag="OO179_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5531310.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15360..16241
                     /locus_tag="OO179_001170"
     CDS             15360..16241
                     /locus_tag="OO179_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5531311.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16447..17916
                     /gene="lysP"
                     /locus_tag="OO179_001171"
     CDS             16447..17916
                     /gene="lysP"
                     /locus_tag="OO179_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5531312.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18208..20199
                     /gene="cirA"
                     /locus_tag="OO179_001172"
     CDS             18208..20199
                     /gene="cirA"
                     /locus_tag="OO179_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR5531313.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20231..21066)
                     /gene="yeiG"
                     /locus_tag="OO179_001173"
                     /pseudo
     CDS             complement(20231..21066)
                     /gene="yeiG"
                     /locus_tag="OO179_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21324..21992
                     /gene="folE"
                     /locus_tag="OO179_001174"
     CDS             21324..21992
                     /gene="folE"
                     /locus_tag="OO179_001174"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5531314.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22009..23085
                     /locus_tag="OO179_001175"
     CDS             22009..23085
                     /locus_tag="OO179_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5531315.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23227..24267
                     /gene="galS"
                     /locus_tag="OO179_001176"
     CDS             23227..24267
                     /gene="galS"
                     /locus_tag="OO179_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5531316.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24547..25545
                     /gene="mglB"
                     /locus_tag="OO179_001177"
     CDS             24547..25545
                     /gene="mglB"
                     /locus_tag="OO179_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5531317.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25606..27126
                     /gene="mglA"
                     /locus_tag="OO179_001178"
     CDS             25606..27126
                     /gene="mglA"
                     /locus_tag="OO179_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5531318.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27142..28152
                     /gene="mglC"
                     /locus_tag="OO179_001179"
     CDS             27142..28152
                     /gene="mglC"
                     /locus_tag="OO179_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5531319.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28188..29401)
                     /gene="preA"
                     /locus_tag="OO179_001180"
                     /pseudo
     CDS             complement(28188..29401)
                     /gene="preA"
                     /locus_tag="OO179_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29395..30633)
                     /gene="preT"
                     /locus_tag="OO179_001181"
     CDS             complement(29395..30633)
                     /gene="preT"
                     /locus_tag="OO179_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5531320.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30827..31066)
                     /locus_tag="OO179_001182"
     CDS             complement(30827..31066)
                     /locus_tag="OO179_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5531321.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31069..31788)
                     /gene="sanA"
                     /locus_tag="OO179_001183"
     CDS             complement(31069..31788)
                     /gene="sanA"
                     /locus_tag="OO179_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5531322.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31938..32822)
                     /gene="cdd"
                     /locus_tag="OO179_001184"
     CDS             complement(31938..32822)
                     /gene="cdd"
                     /locus_tag="OO179_001184"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5531323.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32952..33647)
                     /locus_tag="OO179_001185"
     CDS             complement(32952..33647)
                     /locus_tag="OO179_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5531324.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33644..34042)
                     /locus_tag="OO179_001186"
     CDS             complement(33644..34042)
                     /locus_tag="OO179_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5531325.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34281..35228
                     /gene="dusC"
                     /locus_tag="OO179_001187"
     CDS             34281..35228
                     /gene="dusC"
                     /locus_tag="OO179_001187"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5531326.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35599..35682)
                     /gene="yohP"
                     /locus_tag="OO179_001188"
     CDS             complement(35599..35682)
                     /gene="yohP"
                     /locus_tag="OO179_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5531327.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35906..37342
                     /gene="mdtQ"
                     /locus_tag="OO179_001189"
     CDS             35906..37342
                     /gene="mdtQ"
                     /locus_tag="OO179_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5531328.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37395..38156
                     /locus_tag="OO179_001190"
                     /pseudo
     CDS             37395..38156
                     /locus_tag="OO179_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38273..38851)
                     /locus_tag="OO179_001191"
     CDS             complement(38273..38851)
                     /locus_tag="OO179_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5531329.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPMNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39021..39608
                     /locus_tag="OO179_001192"
     CDS             39021..39608
                     /locus_tag="OO179_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5531330.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39782..40714
                     /gene="pbpG"
                     /locus_tag="OO179_001193"
     CDS             39782..40714
                     /gene="pbpG"
                     /locus_tag="OO179_001193"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5531331.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40752..42467)
                     /gene="dld"
                     /locus_tag="OO179_001194"
     CDS             complement(40752..42467)
                     /gene="dld"
                     /locus_tag="OO179_001194"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5531332.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42663..44960
                     /gene="bglX"
                     /locus_tag="OO179_001195"
     CDS             42663..44960
                     /gene="bglX"
                     /locus_tag="OO179_001195"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5531333.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45212..46129
                     /gene="osmF"
                     /locus_tag="OO179_001196"
     CDS             45212..46129
                     /gene="osmF"
                     /locus_tag="OO179_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5531334.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46136..47293
                     /gene="yehY"
                     /locus_tag="OO179_001197"
     CDS             46136..47293
                     /gene="yehY"
                     /locus_tag="OO179_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5531335.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47286..48212
                     /gene="yehX"
                     /locus_tag="OO179_001198"
     CDS             47286..48212
                     /gene="yehX"
                     /locus_tag="OO179_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5531336.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48217..48948
                     /gene="yehW"
                     /locus_tag="OO179_001199"
     CDS             48217..48948
                     /gene="yehW"
                     /locus_tag="OO179_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5531337.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48929..49036)
                     /locus_tag="OO179_001200"
     CDS             complement(48929..49036)
                     /locus_tag="OO179_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5531338.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49096..49549)
                     /locus_tag="OO179_001201"
                     /pseudo
     CDS             complement(49096..49549)
                     /locus_tag="OO179_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11962 a        12205 c        13311 g        12071 t
ORIGIN      
        1 gagtcattac ccaaaacaga gtggcgataa tcatcaccat cgcaaaccag aacacagcgt
       61 gataattcca gatctcggcg acgatgcccg ccactgatcc tgcgatgatc cagcccacgc
      121 gcgacgtgtt ggtatagagc gtggtggctg aacctgcctg accgggcatc aaatcctgaa
      181 aatagagcat cccgatgccg cccagaatgc caataaaaat agcatttagc agctgcaagc
      241 ccaacagaat ggcaggtgaa tgcgccatca gcattcctgc gtaaaaacag acgccaccca
      301 cggcagcaac gcgcattaag aaacgcttac ccagacgttt ggcgaaatat ccggcaatca
      361 acatcgtcgg gatttccagc ccggcggcgg tccccatcat cacaccggct agtttctcgg
      421 gaagatgcag ttcgttgata ataaatagcg gcatgttgat gatgtacagg ctgttcgagc
      481 cccacatcaa tgtacaaatg acaaacagca gtagcgtatc gcgacggtta cggcgcggcg
      541 cttcaaccgt gccggtcgcc agcggaagct ctttttgcat cgacggtaaa aacagccaca
      601 ccatcacgcc gcaaacaata aacgctaccg ctgcgctcag atacattacc gtaaagctga
      661 aacccatcgc taacgcataa gccagcggtg ggccaatgac ccatgccagt gaaacctgag
      721 cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt tttgtcggca tgttcacggg
      781 caagggcaaa catttgcggg ttagcggtcg agccaaagct gctaagaaag acgccaacga
      841 atagcaaaac aaagtagttg cgattccaag caaaaagggt gcaggccagc acgcctaaca
      901 ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt atcagagcgc ccagcgagaa
      961 actgacttac cagaatccca atgacagcgc tgccggtaaa gaagaatccc accatcgccg
     1021 gacgggcatg tacttcatcg gtaaggaaaa tactgagtgt cggggtttgc agagcacccg
     1081 caataccggt gagaaaggca acgattaaaa acgccgtcga ggtcaggtca aacgatttcg
     1141 cgctggagac tgcgggggag ttatgcatgc tggtcgatca ctggaagagg aagacgcgga
     1201 gtttacgctg cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca cgaataatca
     1261 aaatgccatt tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa ttgctgataa
     1321 cggattttcc catcagcaat taggaaaaat gacaaaaaat tgtgcagcac atcaaacttt
     1381 tgctcataac tttacggctt tccttgcgtg ctgaaacaga agtattatgc tttcttgaaa
     1441 cgtttcagcg cgatcttgtc tttaacccta agctaggttg gcgctttttt tctcatagag
     1501 gctgaatcgt ttcaattcag caagagagga gaactatgtt ccagttatcc gtacaggaca
     1561 tccatccggg cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc gctgcggcgc
     1621 tggtgcaggc cggtaatgta gcagaaggct acgtcaatgg catgctggcg cgcgaacagc
     1681 aaacctcaac gttcctcggc aatggtattg ctattccaca cggcactacc gacacccgcg
     1741 atcaggtgct gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc acctggggtg
     1801 acggtcaggt agcgtacgtg gcaatcggta ttgctgccag ctcggataaa catctgggcc
     1861 tgctacgcca gctgacccac gtactgagcg atgattccgt tgctgaacaa ctgaagtcag
     1921 caacaacagc agaagaactt cgcgcattac tgatgggcga aaagcagagt gagcagctga
     1981 agctcgacaa cgaaatgctg acgctggata tcgtcgccag cgatctgctg actcttcagg
     2041 cgctgaacgc tgcgcgtctg aaagaggcga gggcagttga cgccactttc gtcaccaaag
     2101 ccatcaatga acaaccgctg aatctcggac agggtatctg gctgagcgat agcgccgaag
     2161 gcaatctgcg tagtgcgatt gcggtaagcc gtgcggcaaa tgcttttgat gtggacggcg
     2221 aaacggcagc catgctggtg agtgtggcga tgaatgacga tcagcccatc gcggttctta
     2281 agcgtctcgc tgatttgttg ctcgacaata aagctgaccg cttgctgaaa gcggatgcgg
     2341 caacgttgct ggcgctgctg accagcgatg atgcgccgac cgacgacgtg ttaagcgcgg
     2401 agtttgtagt gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg ctggtcaata
     2461 ccattaaaca atttaacagt gatattaccg tgacaaacct tgatggcacc ggcaaaccgg
     2521 caaacggacg tagtctgatg aaagttgtgg cacttggcgt taagaaaggt catcgcctac
     2581 gctttaccgc ccagggtgca gatgctgaac aggcgctgaa agccattggc gacgctatcg
     2641 ctgctggtct tggggagggc gcataatgag cagacgtgtt gctacgatca cccttaatcc
     2701 ggcttatgac cttgttggtt tctgcccgga aattgaacgc ggcgaagtga acctggtgaa
     2761 aaccaccggt ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat taaaagacct
     2821 gggaattgat gtcaccgttg gcggcttcct gggtaaagac aatcaggatg gttttcagca
     2881 actgttcagc gagctgggca ttgccaaccg tttccaggtt gtacaggggc gcacccgaat
     2941 taacgttaag ctgacggaaa aagacggcga agtgaccgac ttcaacttct cgggtttcga
     3001 agtcaccccc gccgactggg aacgctttgt gactgattct ctgagctggc tcggtcagtt
     3061 cgatatggtc tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag cgttcaccga
     3121 ctggatgact cgcctgcgta gtcagtgtcc ttgcattatc tttgatagta gccgtgaagc
     3181 gttagtagca ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc gcgagttgga
     3241 aatctgggca ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg cacatgcgct
     3301 acgtgaacaa ggtattgcgc atgttgttat ttcactgggt gccgaaggcg cgctttgggt
     3361 taatgcctcc ggcgaatgga tcgctaaacc accgtcagtc gatgtcgtaa gcaccgttgg
     3421 cgcaggggat tctatggttg gtggcctgat ttatggcttg ctgatgcgtg aatccagtga
     3481 acacacactg cgtctggcga cagctgttgc agccctggcg gtaagtcaaa gcaatgtggg
     3541 tattaccgat cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac aaccttttaa
     3601 ctgacagcag gagaggcata atgaaaacgc tgctgattat tgacgctaat ctcggtcagg
     3661 cacgcgccta tatggcgaag accctgctgg gcgcggcggc gcgaaaagca aaactggaaa
     3721 tcatcgacaa tccgaacgac gcagaaatgg cgattgttct cggtgattcc atcccgaatg
     3781 acagcgcgct gaacggtaaa aatgtctggc tgggtgatat ttcccgggca gttgcgcacc
     3841 ctgagctgtt cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg ccggtcactg
     3901 cgacagcacc ggttgccgcc agcggtccga aacgcgtagt tgcggtgact gcttgcccga
     3961 ctggcgtagc acacaccttt atggcggctg aagccattga aaccgaagcg aaaaaacgtg
     4021 gctggtgggt gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca atcactcccg
     4081 aggaagtagc cgcagcggat ctggtgattg tggcggcaga tatcgaagtg gatctggcga
     4141 aatttgctgg taaaccgatg tatcgtacct ctaccggtct ggcgctgaag aaaacctcgc
     4201 aggaactgga taaagcggtt gctgaagcaa cgccgtatga accggcgggc aaagctcaaa
     4261 cggcgaccac tgaaggtaag aaagagagtg caggcgctta tcgtcacttg ctaacgggcg
     4321 tctcttacat gctgccgatg gtcgttgctg gtggtctgtg tatcgcgctt tcttttgctt
     4381 ttggtatcga agcgtttaaa gagccgggta cgttggcagc ggcgctgatg cagattggtg
     4441 gtggttcagc ctttgcgctg atggtgccgg tactggcagg ttatattgcc ttctccattg
     4501 ccgatcgtcc gggcctcacg ccgggtctga ttggcggtat gctggcggtc agcaccggtt
     4561 ctggcttcat tggcggtatt attgcgggct tcctggctgg ttacattgcg aagttaatca
     4621 gtacgcaact gaaactgccg cagagtatgg aggcgctgaa accgatcctg atcattccgc
     4681 taatttccag tctggtggtc ggtctggcga tgatctacct gatcggtaaa ccagttgctg
     4741 gcattctcga agggctgact cactggctgc agaccatggg gactgcgaat gcggttctgc
     4801 tgggggcgat cctcggtggc atgatgtgta ctgacatggg cggtccggta aacaaagcag
     4861 cgtacgcatt cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg gcggcgatta
     4921 tggcggcagg tatggtgcca ccgctggcaa tgggcctggc aacaatggtg gcgcgtcgca
     4981 aattcgacaa agcgcagcag gaaggtggca aagccgctct ggtattggga ctgtgcttca
     5041 tttcggaagg tgcaattccg tttgctgctc gtgatccgat gcgtgtgctg ccgtgctgta
     5101 tcgtgggtgg ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa ctgatggcac
     5161 cgcacggtgg tctgtttgtt ctgctgatcc ctggcgctat tacgccggta ttgggttacc
     5221 tggtagcaat tattgccggt acgctggtgg cgggtttggc ctatgccttc ctgaaacgtc
     5281 cggaagtgga cgcagtagcg aaagcagcgt aataaaaggt gtgttacagg gcagaaattt
     5341 cctgccctgt aacatttgat gagcgcaaag agaatgacca gcttgtgaat gtaattttat
     5401 gaatttaaat ggttttatct ttcagtagaa acttcaaacg ttcatcaaac actaaggtta
     5461 tttcctcaag ggcagattat tttctgccct tttttagtgt tcctgttctg tgcatcccgt
     5521 cacaaattcc tcgcttttgt aattcaggca tttaatcgca ttttgtgagc ctgctcaaat
     5581 ctgcccattt tgcctctctg gcggtaattt actttacgta aaacatgcat actatgagca
     5641 catgtttaaa tatgaacaca tgtttaaacc gtctttgcag tatctatatt cttgtggatt
     5701 gacagggttg gtaatttttt accagaggac gtgctatgcg cgaaaaggat tatgtcgtaa
     5761 ttataggttc ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg
     5821 attcaaatcc aggtaaaata aaatttacgc ctggtggagt agggcgcaat attgcacaaa
     5881 acctggcgtt gctgggtaac aaagcctggc tactgagcgc cgtaggcagt gatttttatg
     5941 gtcaatcgct gctaacgcaa accaatcaat ctggtgttta tgtcgataaa tgcctgattg
     6001 tgccgggaga aaatacgtcg agttatttat cattactcga taataccggt gaaatgctgg
     6061 ttgctataaa tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc
     6121 gtgaatttat tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac
     6181 tggcatggat tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttcagcgc
     6241 ggaaatgtgt caaagtgcgc gaccgtctaa atcagatcca cactctgaag ccaaaccgcc
     6301 ttgaagcgga aaccctgagt gggattgcgc tgtcagggcg tgacgatgtg gcaaaagttg
     6361 ctgcctggtt ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg
     6421 tttattacag cgatatcagc ggtgaaaatg gctggtctgc gccgatcaaa accaatgtta
     6481 ttaatgttac cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg
     6541 gaatgccgtt tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct
     6601 gtgaatacac caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaaca
     6661 cagaatgtct gaattaaaaa tctcccctga attattacaa atttctccgg aagtgcagga
     6721 cgctttaaaa aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat
     6781 gccgttccca caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg
     6841 cgcagtacct gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga
     6901 aaaaattgaa ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt
     6961 accttttgtt gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat
     7021 tgcggcgctt gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg
     7081 ggcggaacat accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac
     7141 cgttgtttgt gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga
     7201 aaccttcggt gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg
     7261 taccagctcg tttgacgtca gcattcgtct cgacagcgcc agtgaaattg cccgtgcaat
     7321 ggcggtgaaa tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga
     7381 acagtttgcg atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc
     7441 cgaagagcag ggcgttattg gtaaagaaag tacaccgttc ctgctggcgc gcgttgctga
     7501 gctgaccggc ggtgacagcc tgaaatccaa tatccagctg gtgttcaaca acgccattct
     7561 ggcgagtgaa attgccaaag aataccagcg tctcgcgggt taatttctga cgggcagaaa
     7621 gccgtcgccc gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca
     7681 tgaagggaaa atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc
     7741 aatagcattt ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc
     7801 actgctgctg caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaaatgggc
     7861 agtagaacag gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc
     7921 cttcattttt ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt
     7981 tatcttcgcc tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct
     8041 gtactacatt ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc
     8101 cctcaacatc agcaaaatcg aatcttttgt tgcggttacc actattttcc tcgggcaaaa
     8161 tgagatcccg gcgatcgtta aaccgtttat cgatcgcgtg aatcgcaacg agttgtttac
     8221 cgcaatttgt agcgggatgg cgtccattgc tggttcggtg atgattggtt atgccggaat
     8281 gggcgtacca attgactacc tgttagcggc atcgctgatg gcgatccccg gcggtatttt
     8341 gtttgcacgt attcttagcc cggcaaccga gccttcgcag gtcacatttg aaaatctgtc
     8401 gttcagcgaa acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg
     8461 gctgaaaatc gccgctggtg tagcgacggt ggtaatggcg tttgtcgcaa ttattgcgct
     8521 gatcaacggc attatcggcg gaattggcgg ctggtttggt ttcgccaatg cctctctgga
     8581 aagtattttt ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga
     8641 tgccaacctt gctggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta
     8701 cctgagtttc tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccgtggcgat
     8761 tatatccttt gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc
     8821 attttcggct atttcgccaa aacgtgctcc agaaatcgcc cagcttggtt tacgggcgct
     8881 ggcagcagca acgctttcca acctgatgag tgcgactatt gccgggttct ttattggtct
     8941 ggcgtaatgc tcgatttgta gaccggataa aacgtattaa gcgttgcatc cggcaatcgc
     9001 gctattgcaa ataattttta ctgcatcatc ccggagaatt tattctccgg gtccatctcc
     9061 agcgccagtc ctgacaactg ctttctgttt ttaattagat atcctttctt ttaattaata
     9121 aaccgtcgtg aatgaattgc gcgagaacat ataaaagatg tcgataagaa acgcctaaat
     9181 actctgccgc ttgcgtatgc ttttcgtgat aaagatcacc ttcttgtgag agtaatataa
     9241 atgctgccag gcgattcact aacggaaatg attgattctg agttaaagaa acaatattac
     9301 gataattttt atgacttaag gtgacgcaaa gttttcgtaa aaatagggtg tcgtttaata
     9361 acagcggacg gtaatgtttc atagggagcg caaggcacca acactcttca atagcctgca
     9421 ccgcacgcgg ttcatggtct ttgtcgatta actcaatctc gccaataaaa cagggagcgg
     9481 caaagaaatc gatcagcgac acgcgaccat tggcaagcgt ggcgtaaagc ctggcgcggc
     9541 ctcgcgtcag ataaaacagc catgacggct gttgcccttc ctgcacaatg tagtcacgcg
     9601 ctaaaaaatg aaacagccgc gtatcggctg aaacatccgt tgaaaagcaa tccttaaacg
     9661 cagattcact catcaattgc tgcttaaggt cattattgtg gatttctttc acgatgttta
     9721 tccgctatga gatttctcat atttagcata cgtacgctgt tgttaagatt caatcattgg
     9781 cccggtcgaa ttttctttgc tgtagaaaaa aataacgcaa ctggaaacag aggaaataaa
     9841 caatggaaaa gagaaaaatt attctggatt gtgagccggg tcatgatgat gctattgcaa
     9901 tgatgatggc agcgaaacat ccggcaatag atttattagg catcactatt gtagcgggta
     9961 atcagacgct tgataaaaca ttaattaatg gcctgaatgt ttgccagaaa ctggagatta
    10021 atgttccggt ttatgcgggg atgccgcaac ccattatgcg taaacaaatc gttgccgata
    10081 atattcacgg tgaaaccgga ctggatggcc cggtattcga gccgctgacc cgctaggcag
    10141 aaagtaccca tgcggtgaaa tatatcatcg ataccctgat ggcaagcgat ggcgatatca
    10201 ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg caacccgcaa
    10261 tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca ggcaacttca
    10321 ccccatctgc cgagttcaat atctttgccg acccggaagc cgcccgcgtg gtgttcacct
    10381 ccggcgttcc attagtgatg atgggcctcg atcttaccaa ccagaccgtt tgcaccccgg
    10441 atgtaattgc tcggatggaa agggtaggcg gccctgccgg agagctgttc agcgacatca
    10501 tgaacttcac tctcaaaacg cagttcgaat actacggcct ggctggcggc ccggtgcatg
    10561 acgccacctg cattggttat ctgatcaacc ctgatggcat taaaacccag gatatgtacg
    10621 tcgaagtgga cgtcaacagc ggcccttgct acgggcgtac ggtctgcgac gaactgggcg
    10681 ttcttggcaa gcccgctaat accaaagtcg gcatcactat tgataccgac tggttctggg
    10741 gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg ataacaagcc
    10801 gggacagtat cgcaagatgc aggcccggct atttctgatg ggcagaaagc cgtcgcccaa
    10861 aatgacatga agagataatc actatggatg tcatgagaag tgttctggga atggtggtac
    10921 tgctggcaat tgcgttttta ctgtcagtaa acaagaagaa gatcagcctg cgtaccgttg
    10981 gcgcggcgtt agtgttacag gtcgtgattg gcgggattat gctttggtta ccgccagggc
    11041 gttgggtcgc agaaaaagtc gcttttggcg tccacaaagt gatggcgtac agcgacgcag
    11101 gtagcgcatt tatcttcggt tctctggtcg gaccgaaaat ggatacctta tttgatggcg
    11161 caggatttat ctttggtttc agggtattac cggcaattat cttcgtcact gcactggtga
    11221 gtattctcta ctacatcggt gtgatgggga tcttaattcg cattctcggc ggtatcttcc
    11281 agaaagcttt aaatatcagc aagatcgagt cattcgtcgc ggtcaccacc attttcctcg
    11341 ggcaaaacga aattccggcg atcgtcaaac cctttatcga tcgtctgaat cgcaatgaat
    11401 tatttacagc gatttgtagt ggcatggcct cgattgctgg ttcgacaatg attggttacg
    11461 ccgcactggg cgtacctgtg gaatatttgc tggcggcatc gttaatggcg atccctggcg
    11521 ggatcttgtt tgcccgcctg ttaagcccgg ctacggaatc ttcgcaggtt tcttttaata
    11581 acctctcttt caccgaaaca ccgccaaaaa gcattattga agccgatgcg acaggggcaa
    11641 tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt tatggcattt gtcgccatca
    11701 ttgcgttaat taacggtatt atcggcggcg ttggcggctg gtttggtttt gaacatgcct
    11761 cgctggagtc cattttaggt tacctgctgg ccccacttgc gtgggtaatg ggtgttgact
    11821 ggagtgatgc aaatcttgcc gggagtttga ttggacagaa actggcaata aatgaatttg
    11881 tcgcttatct caatttctca ccctatctgc aaacggctgg cactctggat gctaaaaccg
    11941 tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt tggttctatc ggggtggtgg
    12001 tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga aatcgcccag cttggtttac
    12061 gcgcgctggc ggcggcgaca ctttctaatc tgatgagtgc caccattgcc gggttcttta
    12121 ttggtttagc gtagtgtgca atttatgagc cggattagat gcggtcaagc gtcgaatccg
    12181 gcaacattgt tacaccattg gcactaatga cagcgcgtta tcggcagaca gggtagggtt
    12241 gtttaagctg ccgctggcgc gtgaaattgc cgcgcaggcc atcgcaaaac gggcgctgtc
    12301 acggaaattg tttccctcca gaaagctata taccagaccc gccataaaac catcgtcagc
    12361 accaaaactg tctaccgtcg tatgcgctgg cacagtcagc aaaaattgtt caccatcctt
    12421 ttcgctgcaa taaactgact catcgggcaa ataaacaaac agttgctgaa caccttgctg
    12481 atgcaatgca ttcactgcgg cattacggtc agcatcgctg gtgatcgcct gtccccataa
    12541 aatttccagc tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc agtgtttgat
    12601 tttgcccgct ttgaattctg aaacggtatc gacaaacacc gggatctcat cagcgagggt
    12661 aaagacccat tccagcgcct cggctgtcag gttgcaatca gccagtacca cgcccgcatg
    12721 acgaagtaaa tcgcgcgacc cgttcagtag ttgcggtgtc aactgttcca gcagatgggt
    12781 atcgttaatt gccagcacgg tttgatcgtc tcgattggca attgccagat acgtcgatgt
    12841 gctttgacca tgcaaacgaa cgcagccgga gacattcacg ccggcacggc gcgtttcttc
    12901 caggagcatt tcgccataaa agtcatcgcc aatcactgaa agcaaatgga cgtcacggcc
    12961 taacagcgcc agattgtggg cgatattgcg tccaacgccg cctgccgaac aatgaattgt
    13021 accgggatga gaagccgctt gcgggtaatg gatatccgcc atcccgcgaa tatccatatt
    13081 gattgtcccc accactacgc agtattcctg ctcggtgaga atgtaacctt tgtctttaat
    13141 ccggcctttg cgcattaaat ccataatatg cgccgcaacg cgcgaacgac tgatttgcag
    13201 catgtccgca atttcgttct gctgaatcag cgggttacgc cgtaaaattg caaggatctc
    13261 cttttcccgg ttattcatct tcaggctacc gctttttcag tttgttgcgc tttcagccag
    13321 gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg
    13381 aaacggtcgt tctgcataat ccagcggaac gcatcatgac cgatattgcc ttcaccgagg
    13441 ctatgatggc ggtcaacgcg gctgccaaag gtacttttcg catcgttaag gtgcatcccg
    13501 cgcagatact taaagccgac aatacgggca aaatcagcga acgttttttc gcattcggct
    13561 ggagtatgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgacg
    13621 cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag
    13681 ttactgccct gaccggcggt gttttcaatc accgctgtta caccttgagt tttatccagc
    13741 gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga
    13801 tggctgccag ggtggaagtt gagcaaagaa agccccagtt gttcgcaacg ctgcatttca
    13861 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttgatc
    13921 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
    13981 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
    14041 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
    14101 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
    14161 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg tttatgcgat
    14221 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
    14281 cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt gagtggtcag
    14341 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
    14401 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc
    14461 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
    14521 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
    14581 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
    14641 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
    14701 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
    14761 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
    14821 ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct tatccagacc
    14881 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc
    14941 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
    15001 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
    15061 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
    15121 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
    15181 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
    15241 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
    15301 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
    15361 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
    15421 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgactgacc
    15481 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
    15541 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
    15601 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
    15661 actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
    15721 aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
    15781 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
    15841 acgagcaggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
    15901 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggatattg
    15961 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
    16021 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg
    16081 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
    16141 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
    16201 gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta caagaacatg
    16261 ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca gaaaagagat
    16321 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
    16381 gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga tggatagcat
    16441 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
    16501 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    16561 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    16621 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
    16681 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    16741 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagcacag
    16801 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    16861 ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
    16921 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
    16981 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    17041 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    17101 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    17161 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt cgcgatcctg
    17221 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
    17281 atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    17341 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
    17401 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    17461 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
    17521 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    17581 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    17641 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    17701 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    17761 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    17821 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
    17881 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
    17941 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    18001 ttaatatttg gattgataat tgttatcgtt tgcattatcg ttacgacaca atcaaaaaag
    18061 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
    18121 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
    18181 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
    18241 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
    18301 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc cgccagtatc
    18361 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
    18421 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
    18481 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
    18541 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
    18601 cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc gctcggcggt
    18661 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
    18721 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
    18781 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
    18841 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
    18901 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
    18961 actgccggat acggtttcga ccgtcaggat cgtgattccg attcgctgga caaaaaccgc
    19021 ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg caccagcgaa
    19081 ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
    19141 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
    19201 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
    19261 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
    19321 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
    19381 gaacactgga gtccgcgtgc ctacatggtt tataacgcca ccgacaccgt aacggtgaaa
    19441 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
    19501 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
    19561 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggt
    19621 gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
    19681 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
    19741 ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat tcagggcgtg
    19801 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
    19861 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
    19921 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
    19981 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
    20041 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
    20101 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
    20161 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
    20221 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
    20281 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
    20341 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
    20401 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
    20461 gcgcgcaact atcccattcc agcatgcatt tttgtcctca cctaaatagg tgctaaacgc
    20521 tttgattccc cacgggacgc tgcacggatt cacaatgggc gcaaaggccg aaacgctggt
    20581 gtatttaccc ggatttttca gcgccataat cagcgcaccg tgaccaccca ttgagtgacc
    20641 gctaatggcg cagcggtcgc tgacattaaa ttgcgactga accagcgccg gtaattcatc
    20701 gcgcagataa tcatacatcc ggtaatgcgt cgcccacggc ggttgcgtgg cattaagata
    20761 aaagcctgcg ccctggccta aatcgtagcc atcgtcgttg gcaacctttt cgccgcgcgg
    20821 gctggtgtct ggcatcacca gtacaatccc cagttccgcc gctacccgct gggcacccgc
    20881 cttggtggtg aagttctcgt cattgcaggt taatccggaa agccagtaca gcactggtgg
    20941 cggggtgtga tcacgagggg gtgggagaaa gatactgaac gtcatcgggc agtttaaggt
    21001 actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct cttcgagcat
    21061 ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg tttattcaat
    21121 gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat ctgctgatga
    21181 aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc gccgcctcac
    21241 gcacaataat caagctgtaa atcagcttaa taactttgcc cccacgcagg gcggaggcgt
    21301 cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc ctggttcatg
    21361 aagcgttagt tgcgagagga ctggaaacac cgctgcgccc gcccgtgcat gaaatggata
    21421 acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag ctgctgaatc
    21481 tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg
    21541 atgaaatttt ctccggtctg gattacgcca atttcccgaa aatcaccctc attgaaaaca
    21601 aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac
    21661 accattttgt taccatcgat ggcaaagcga cggtggctta tatcccgaaa gattcggtga
    21721 tcggtctgtc aaaaattaac cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg
    21781 aacgtctgac gcagcaaatt cttattgcgc tacaaacgct gctgggcacc aataacgtgg
    21841 ctgtctcgat cgacgcggtg cattactgcg tgaaggcgcg tggcatccgc gatgcaacca
    21901 gtgccacgac aacgacctct cttggtggat tgttcaaatc cagtcagaat acgcgccacg
    21961 agtttctgcg cgctgtgcgc catcacaact gattaaaagg caggaaccat ggagcgcaac
    22021 gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt aaacatcagc
    22081 gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacgatgc tattacgccg
    22141 caggatgcat ggacctgggc atttctcgat ctcatcggcc aggtgaaatt cctcacgctt
    22201 tttgcgctgc tgtttggtgc tggcctgcaa atgttgctgc cccgtggcag actctggatc
    22261 cagtcgcggt taacgctgtt agtcttgttg ggctttattc acggtttatt gttctgggac
    22321 ggcgatattc tgctggctta cgggctggtg gggcttggcg ttttgctgtt attggggctg
    22381 atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa
    22441 aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc
    22501 aacagtttac tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt
    22561 ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt
    22621 cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg
    22681 caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa
    22741 ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa
    22801 ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac
    22861 tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat
    22921 tttgaccgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc
    22981 tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta
    23041 actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggattacat
    23101 tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta
    23161 ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta
    23221 gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt
    23281 tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa
    23341 gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt
    23401 agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta
    23461 aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat
    23521 catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg
    23581 ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat ggataacatt
    23641 cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg
    23701 gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt
    23761 attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg
    23821 atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg
    23881 ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt
    23941 accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat
    24001 aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc
    24061 cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta
    24121 gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt
    24181 tttatgccga cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc
    24241 actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc
    24301 ttaacaatgt gatagctatg attgcaccgt tttaatgttg taacccgtat gtaacagtga
    24361 ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt ttctggagcg
    24421 ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat
    24481 taactctact gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga
    24541 gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt
    24601 gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac
    24661 tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag
    24721 ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg
    24781 ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg
    24841 gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct
    24901 cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc
    24961 ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg
    25021 aacaaagacg gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca
    25081 gaagcacgta ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag
    25141 ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat ggacgcctgg
    25201 ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca
    25261 atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc
    25321 gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg
    25381 aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc
    25441 aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat
    25501 gttggcgtag ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca
    25561 gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg
    25621 actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt
    25681 gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg
    25741 ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa
    25801 gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca
    25861 ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg
    25921 atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa
    25981 atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg
    26041 cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc
    26101 tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc
    26161 aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc
    26221 tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt
    26281 cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg
    26341 gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg cgaagtcatc
    26401 ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat
    26461 ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt
    26521 gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa
    26581 cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag
    26641 gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc
    26701 aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat
    26761 acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt
    26821 tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca
    26881 gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt
    26941 tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa
    27001 atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc
    27061 ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac
    27121 ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg
    27181 aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat tttccaggac
    27241 ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt
    27301 attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt
    27361 cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc
    27421 aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt
    27481 gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg
    27541 ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac
    27601 gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag
    27661 ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg
    27721 gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt
    27781 ggcggtaacc cggaagcggc aaaaatatct ggtgtcaacg tcggcctgaa cctgctgatg
    27841 atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc
    27901 ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg
    27961 gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta
    28021 attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag
    28081 tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg
    28141 cgtaagaagt gatgtgattg cggatattgc cggatgcgtc tctggtttta caacgttacc
    28201 gggtgttctt tctcgccttt cttaaacttc acttccccga gctcaataca acccaccggg
    28261 cagacgtgac cacaaagcag acaacccaca catttctcgg tattacaatg cggtgtgcgg
    28321 gttttctcgc tccattccat cgcctggtga ccgccgtcgt aacaggaaat ataacagcgt
    28381 ccacagccaa cacatttgtc aagattgata cggggataga caatataact gcggtctaaa
    28441 tcttccgccg ggacaatatt gttattcgcc agacctacca tctcctgcaa cgaatcaaat
    28501 ccctgatcgg cgagataatg cgataacccg ctcgccatat cttctacgat gcgatagccg
    28561 tactgcataa tgccggtggt gacctgtaac gttgctgcgc cgagcaataa aaactccgcc
    28621 gcatcttccc aggtttcaat gccgccgata ccgctgattg ggaaatcgcg cagttctgga
    28681 tgggtgcgca tttgctgaat gaagcgcagg gcgatcggtt ttaccgcttt accggaatat
    28741 ccggaaatac tcgatttacc gttaacgatc ggcataccga ttttctgatt aagatcgata
    28801 ttggtgatgg atttaacggt gttaattgcc gcaatgccat ctgcgccgcc gcgcttcgcc
    28861 gccagcgcca cttcgcacat atcaccgata ttcggcgtca tcttcgccag cattggcagc
    28921 gtggaacccc gtttcactgc ccgacaatat ttttctacca gctccgggct ttgcccgaca
    28981 tcgctaccca tcgcatgaga agtcatttgc ggacaggaga agttacactc gatcatatcc
    29041 gcgccagctg ctcccattgc tgctcatttt cccccatgat cgaagcgatc aatactttgt
    29101 ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt ctcttccaac ggatgttcag
    29161 caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa
    29221 aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acaccgcccc
    29281 aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga
    29341 ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca
    29401 tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac
    29461 ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcggatc
    29521 gcgggtctga taattttgtg ttttgatggt gttacgctgc tgctctaact ccgcaaaggc
    29581 atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc
    29641 gcctgataac cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa
    29701 tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt cgcttgccgg
    29761 aaactcatct aactcttcac gtgctacgca agttaccgcc tgacagccga gaactttcag
    29821 cgtgctggct acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat
    29881 gctgatatcg ccttgcgcct gtcgtacacg ttgcaggaag tcgacggcaa tctcaacgtc
    29941 gctatgctca aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc
    30001 gcggttttcc gctttcagtt gctccagggt gagtgtttta ccgatttcgt tattgcactt
    30061 aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg attgcggtaa
    30121 ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt tctcataaat
    30181 cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg caggacctgc
    30241 gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt
    30301 ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac
    30361 accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac
    30421 ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta
    30481 gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc
    30541 gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg
    30601 agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat
    30661 tatggcgaac gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt
    30721 taagctgtga aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg
    30781 agtgaagtaa taattattct caataattaa cagaaaagag aaaatattaa cgatagaaaa
    30841 taagataaac aatcatgccg atagataaaa ttccgaagcc aatatatgcc accatataag
    30901 cgaagaagag tccgggattt tttgctcgcc agacatacac cggttccggt gcatttttaa
    30961 cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg
    31021 ggataaggaa aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg
    31081 tttcttttgt aattcaagta actgttcggg tgtaacggcg ggatacccct gcgcatcttc
    31141 cggcacctgg tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt
    31201 aaaaatataa aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa tacgtactga
    31261 cagcatatct ttcggtgacg gtacggcata acactgagcc tgaatcccca tatgcagcgc
    31321 aataaataat gctcgctcac agtggaaacg ttgggtgata ataatgaaat cattagtatc
    31381 gaaaactttg cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt aatcgagaac
    31441 aatatctgat gggtcgacac cagcagcgat taaatctttg cgcatggtca tcggctcgtt
    31501 ataactttgc aatgcgttat cgccgctcag taatagataa tttaccttac cgctgttata
    31561 ggcattaatc gctccttgaa tgcggtagcg ataatactga ttaataacgc cggtacgata
    31621 atattttgct gtgccgagca ccacgccgac ctggcggtag gggagatcct gcaattcgtc
    31681 gtagatataa ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag
    31741 cagcaagccg atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg
    31801 agctaaggaa aatttccatc aggctacttt acccgctggt tttgcgcaag aaacggcaaa
    31861 ctgtttgccg ggaatttcga caccaggaga gggctggcaa gccggacaag gcgttcacgc
    31921 cgcatccggc accaggctta agcgagaagc acgcggtcga tactgtgaca gccgagagct
    31981 ttcagcgttg cggaggtggc atcccactga atcaacggcg catcggcttt ttctgccaga
    32041 accgcgcgct ggatatccgg gtaatcataa cccttgagat tcaacagaat taacgccccc
    32101 tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata
    32161 cgaccgtctt tacattccag cgcgacaccg cttggtgact tactgtaagg catgtgcgaa
    32221 cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc atagccgtga
    32281 tcctgctcgt ccatcagcag cgttttaatc tccagatctt tcggcccaaa ggcatccggc
    32341 agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat gaatacgcag atccagaccg
    32401 ctgttcagtt cattcataaa ctgacggcag tgaccacaag gcgtatagtt aacggtgatg
    32461 gctgcaagcg ctttttcacc actcaaccaa gcgtggctga ttgcgctttg ttcggcatga
    32521 acggtttgct gcattgttgc accaataaat tccatattgg caccaaaata ccaggttccg
    32581 ctcacaccgc gcgcaattgc gccaacatta aaattcgaca atggcgtacg cgcacaggcc
    32641 gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt ccagtcccgt tgcgctcttc
    32701 agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc cagaataggt
    32761 tccagtgcag attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa acgtggatgc
    32821 atggcgttgc ctcataatca tgtaatggga taacagttta cgaacccgtt atggctttat
    32881 atgtgatctg aatctcatta attatgcaac acgtgaaact tacatcaaaa atgcgcgacg
    32941 tatcgcaaat tttagcccat tactgccaga ataatcggga aaaggaacgg tgcgatcagc
    33001 gaagtaatta tcccgcataa caccagcgct agcgaactaa atgcaccttc ctgataatcc
    33061 agctcggcgc agcgcgccgt accgagggcg tgcgaggcag tccccattgc cagaccgcgc
    33121 gcagctttgg tacgaatacg catcgcatta agcaatgtat ggccaaatac cgcgccgagg
    33181 atgccgacga aaatcacgca aactgcgctg attgccggaa taccgccaat actgccgcca
    33241 accgccattg caataggcgt ggtgactgat ttcggcagga ttgacgcggc gatttccggt
    33301 gaagcgccca tcaacaatgc cacggaagtc cccgtcacca tcgcaaccac gctgccgata
    33361 aaacagatgg tgatgatcga tttccagcgc gcgcggatct ggtgtagctg ctcatataaa
    33421 ggataggcca gcgcgacgac cgccggttgc agcagatcgt ttagcacttc gctaccttta
    33481 aagtaactgt cgtaagagat gccggtcagc attaaaaaag gaatgatcac caccattgct
    33541 accagcaacg ggttaagcag gggaaactta taccgtgccg ccagtttgcg ggcggcaaaa
    33601 aagacaatca acgtcagcgg taatgaccac cagatattcg ccatcattct tctgatcctt
    33661 tctgacctac gactttacgt tcaccgtgta ccagttgtga actccagctc accaccagaa
    33721 aaaccaccag cgtactgaca gcacaggaaa ccactaccgg gccaaactgc gcgcggagca
    33781 aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc atatagcgaa
    33841 tcagtacgta gcaccccgga ttgacccatt tcgccggaag aatttgcaag gccagcagga
    33901 caaacaggat ctgcatcccg atgatgctgc ccggaatggt taccggtagc agggaagcaa
    33961 tgaaaatgcc tgcatacagg caggcataaa tcaggatgaa agcgcgtaaa tattgccaga
    34021 taatgttcag tgtcttactc ataatcaaca ccccagatga gatggattca tcatacaatt
    34081 aattcgtaaa acgtgctacc gatcacatta agaaatcagc gcttattttt tcgctacccg
    34141 gtattacgca atgagcagcg aactcaggca tacatcaagc atagagaggg attgcagttt
    34201 gcagcaatcc ctctttgcag acctttaatc cgctgaccat aggcgctaac atagcgcctc
    34261 atttttttgc gggtgatgat atgcgtgtgt tactggcacc gatggaggga gtgcttgact
    34321 ctcaggtgcg tgaattgctg accgaagtta acgactacga tctgtgcatc accgagtttg
    34381 tccgcgtggt ggatcaactg ctgccggtaa aagtctttca tcgcatttgc cctgagctac
    34441 aaaacgccag ccggacacca tctggtacgc tggtgcgcgt gcagttgtta ggtcagttcc
    34501 cacaatggct ggcagagaac gccgcccgtg cggtggagtt aggtttctgg ggcgtggatc
    34561 tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg cggcggggcg acgttactca
    34621 aagatcctga actcatctac cagggggcaa aagcgatgcg tgaagctgta ccggcgcatt
    34681 tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg tgagaagaaa tttgaaatcg
    34741 ccgatgcggt tcaacaggct ggcgctacgg agctggtggt gcatgggcgg acgaaagagc
    34801 agggttaccg cgcggagcat attgactggc aggcgattgg cgagattcgc cagcggctga
    34861 atattccggt gattgccaac ggtgaaatct gggactggca gagcgcgcaa caatgcatgg
    34921 cgatcagcga ctgcgacgca gtgatgattg gtcgcggggc gttcaatatt cccaacctga
    34981 gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt gctttgctgc
    35041 aaaaatatac ccgtctggaa aagcagggcg ataccgggtt atatcacgtt gcgcggatta
    35101 aacagtggtt gagttatttg cgtaaagaat acgatgaagc aacggaatta tttcagcatg
    35161 ttcgggtgtt gaataactcc cctgatattg caagggctat tcaggcaatt gatatcgaga
    35221 aactctaaaa accatagtga tgtttttgac aatgtgttag ctctttatca ataggccgca
    35281 cggttgtttg gtggcgtcaa aatcgccgag gcgttccctg aaggccgggg cagcccacat
    35341 ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgacc cggtagccgt
    35401 aagggataag ccgagggcac cgcgcagcgg cgattttgtt cgccagagcc cgggggggca
    35461 gggggcggcg gcgattggtc gccccctgcg cgctccttgc gccagtgatt atatgaagca
    35521 atgcacatga caggagcgca acaggatgag agactgaaat attctcgtta ttttcggatc
    35581 tgatgacatt aattaatttt aaaatatcat cttaaatacg ccagtcacca ccagtagccc
    35641 aatcaggaaa ataatcaata cagcccagag tataattttc attggcgttc ctttatttgt
    35701 gagtgctgaa taagtatagg aaaaaacgaa gtcctttgcc gttatccata aacgattaag
    35761 ataaatcgga agatatcatt tacttagtct atatttttct gggtatgtcg ttttattgct
    35821 actgatgtat tgccacgcga aactaaaaaa tatatcgcaa cacacggtaa tctccgcgca
    35881 ccgtttctta cctgattatt cccccatgaa tcgtgattcc ttttatccag ccatcgcctg
    35941 ttttccgctg ttactgatgc tggccgggtg tgcgcctatg catgaaaccc gccaggcgtt
    36001 aagccagcaa acgcccgctg cacaagttga caccgcatta cccacggcgc tgaaaaatgg
    36061 ttggccagac agccaatggt ggctggagta tcacgataat cagctcactt cattaattaa
    36121 caatgcacta cagaacgcac cggatatgca ggtggctgaa cagcgcattc agctagctga
    36181 agcccaggcg aaggcagttg ccactcagga tggtccgcag atcgactttt cggcggatat
    36241 ggagcggcaa aaaatgtcgg cagaagggct aatggggccg tttgctctga acgatccggc
    36301 cgcaggtacg accggtccgt ggtacaccaa cggaactttt ggcttaacgg cgggctggca
    36361 tctcgatatc tggggaaaga atcgggcgga ggttactgcc cgcctgggta cggttaaagc
    36421 acgggcggcg gaacgcgagc aaacccgcca attgctggct ggcagcgtag cccgcctgta
    36481 ctgggagtgg caaacccagg cggcgttaaa cacggtcttg cagcaaatag aaaaagagca
    36541 gaacaccatt atcgcgaccg atcgccagct atatcagaac gggattactt cttcagttga
    36601 cggtgtggaa accgatatta atgccagcaa aacccggcag cagctcaacg atgtcgcggg
    36661 gaaaatgaaa attattgagg cacggttaaa tgcactcaca aatcaccaga ctaagtcatt
    36721 gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg aactggggta
    36781 ctccttactg gcccggcggg cagatttgca ggcggcgcac tggtacgttg agtcatcgtt
    36841 aagcactatt gatgcggcaa aagcggcatt ttatcctgac atcaacctga tggccttcct
    36901 gcaacaggat gcgttgcact taagcgatct gttccgtcat tccgcgcagc aaatgggcgt
    36961 tacggcaggc ctgacgctac ctattttcga tagtggtcgt cttaacgcca atctcgatat
    37021 cgcaaaagcc gaaagcaact tgtctatcgc cagctacaac aaagcggtgg ttgaagcagt
    37081 gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa accagcatca
    37141 ggcgcaaatt gagcgcgatg ccttgcgtgt ggtaggtctt gcgcaggccc gctttaacgc
    37201 gggcatcatt gctggttccc gcgtcagcga agccagaatc ccggcgctgc gtgagcgggc
    37261 caatggcctg ttattgcaag ggcagtggct ggatgcctcc attcgactca ccggtgcgct
    37321 gggcgggggt tacaaacgct gatgatatgc gccttctata cttaacgttt attcagcgtt
    37381 aagtggagaa ctcgatggca taggtcgcga taattaccgc ttccgattcg gggatcggca
    37441 aagagtgcgc gttattactg gcgcagcagg ggtttgatat tggcattacc tggcactcag
    37501 atgaagaagg ggcaaaagat accgcgcgtg aggtggttac ccacggcgta cgtgcggaga
    37561 tcgtgcaact ggatctcggc aatctgccag aaggtgcgca ggcgctggag acactcatcc
    37621 agcgatttgg gcgtattgat gtgctggtga ataatgcggg tgcaatgacc aaagcgccgt
    37681 ttcttgatat ggcttttgat gagtggcgca agatttttac cgttgatgtt gatggtgcat
    37741 ttttatgctc gcaaattgcg gctcgtcaga tggtgaaata agggcagggc ggtcgcatca
    37801 tcaacattac gtcggtacat gaacatacgc cgctgccgga tgccagcgcc tacacagccg
    37861 ctaaacatgc gctcggcggg ttaaccaaaa cgatggcgct ggagttggtc aggcataaga
    37921 ttttggtgaa cgcagtcgcg cctggggcga tagccacgcc aatgaacagc atggatgata
    37981 gcgacgtgaa gcccgacgcg gagccttcga ttcctctacg gcgttttggc gcaacgcatg
    38041 agattgccag cctggtggtg tggctttgtt cggagggcgc aaattacacg acagggcagt
    38101 cgttgatagt ggatggcggc tttatgttgg cgaatccgca gttcaaccca gaatagcgca
    38161 gtgaggtgcg tggatatttc aatgggttag gccagataag acaccagcgt cgcatcaggc
    38221 atggaacacc cggtgccgga tgtggagtga atgcttatct gtggcaactg ctttacgcct
    38281 gattatccgg ctttttcttc ccgcgccgtt tcagccacca gcgcacgccc accaccagaa
    38341 ccacgaccag aatcaaccag acccagtgct tcaaatgctg gtcgagattg tgcaaccacg
    38401 gcgcaatcac ctgaccacca gcgtaaccaa tagtggtaaa aatcaacgcc caggcaaatg
    38461 cgccgagaat attcatcggc agaaagattt tcggcggcag ctggctggca ccaatcagcg
    38521 tcgggccaat cacccgaaag ccatacataa agcgcgtacc aatgacaaac agatacggat
    38581 ggcgttggat aagtttctgt gcccgctcaa ttttatcctg atgtttcgag aaacggcgta
    38641 acagcttgcc gccaaaccgc cgcccgcaca gatagagcac ctggtcgcca atcatgccac
    38701 caagcgccac agaaagtacc accagtggga actttaatag cccctgatgt gccgcaacgc
    38761 ctcccagcaa agtcacggtt tcaccttccg ccaggctacc gataaccagc gcggcataac
    38821 catattgtga gatgagtgta ttgagagcca taggccagtt tgtctccctt tggttgcctg
    38881 taagcataca ccctgtgaat cattcacgct gaaaaaggac atacatcaga cggtgcaaat
    38941 tacatggatg aacaaaaaat aatcacaagc tggcttatac ttgaactgtc aggttcatga
    39001 actcacaaca agggggcggt atgagccatg tctggggact gttttcccat cccgatcgtg
    39061 aaatgcaggt gattaatcgc gaaaacgaaa cgatttctca ccattatacc caccacgttt
    39121 tgctgatggc ggcgattccg gtgatttgcg ccttcattgg cactacacag attggctgga
    39181 attttggcga tggcactatc ctgaagttat cctggtttac cggactggcg ctggctgtct
    39241 tgttttacgg cgttatgctg gctggggttg cggtgatggg gcgggtcatc tggtggatgg
    39301 cgcgcaatta cccgcagcgt ccgtctcttg cgcactgcat ggtcttcgcg ggctacgttg
    39361 caactccgct gtttttaagc ggtctggtgg cgctttatcc actggtctgg ttgtgtgcgc
    39421 tggtcggcac ggtggcgctg ttttacaccg gctacctgct ttaccttggt attccgagct
    39481 tcctgaatat caacaaagag gaaggcctta gcttctccag ttcgacgctg gcgattggcg
    39541 tgctggtact ggaagtgctg ctcaccctaa ccgttatact atggggctac ggttatcgtt
    39601 tgttctagaa agttctttga cgttgcattg ctggcgtaaa tgtcagcaat gcagcattta
    39661 ctcacgattc tcctttgacg atcgcacttt ttgctcgtta tgatggcgtc gctagcctca
    39721 gtaaatcctt actccggcgg tgcgcaaccc gtgcgcgtga accactatct gaatgctcat
    39781 catgccgaaa tttcgagttt ctttatttag cttggccctg atgctggctg tgccttttgc
    39841 accgcaggcc gttgcgaaaa cggcagccgc taccaccgct tcacaaccgg aaattgcctc
    39901 cggtagcgcg atgattgttg atctgaatac caacaaagtg atctattcga accacccgga
    39961 tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc gcgatggttg tgctggatgc
    40021 acgactgccg ctggatgaaa aactaaaagt ggatatcagc cagacgccgg agatgaaagg
    40081 ggtctattcg cgcgtacgac tgaatagcga aatcagccgt aaagatatgc tgttgctggc
    40141 gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg caccattatc ccggtggtta
    40201 caaagccttt attaaggcaa tgaatgcgaa agcgaaatcg ctcggaatga acaacacgcg
    40261 ctttgttgaa cctaccggat tgtcggtgca taacgtttca actgcccgtg acttaaccaa
    40321 actgctcatt gccagcaaac aatatccgtt gatcgggcag ttaagtacta cccgggaaga
    40381 tatggcaacc ttctctaacc cgacgtatac gctgccgttc cgcaatacta atcatctggt
    40441 gtatcgcgat aactggaata ttcagttaac caaaaccggc tttaccaatg cggcgggcca
    40501 ttgtctggtg atgcgtacgg ttatcaataa taaaccggtg gcgctggtag tgatggacgc
    40561 gtttggcaaa tatacccatt ttgccgatgc cagccgcctg cgtacctgga ttgaaaccgg
    40621 taaagtgatg cctgtgccgg cagcagcgtt gagctataaa aaacaaaaag ccgcccaaat
    40681 ggcggcggcg gggcagacgg cacagaacga ttaagtgaat tcggatggcg atactctgcc
    40741 atccgtaatt tttactccac ttcctgccag tttttccgtt tactggtttt accgatcccc
    40801 ggattcatgc tattggtcgg atcattctcg cgatagaact tctgcaacgt ctccggtgct
    40861 ttatacaaat gaccgacgtt atgctcggca gggtactgcg cgccgcgctg ctgtagcagt
    40921 tccagcatct gttcttttaa cgcatgcaca tccacgcctt ttttcactat gtaatcctga
    40981 tggaagacat agcacataaa atggccgtaa tagagcttgt gcaccagctg gctgtcgatc
    41041 tccggcggta aatgctcata ccactcggtg tcgttacgcc gcagagcgat atccagtgcc
    41101 agaatgtctt cgacttcatc ggaatgcacc gcctgataac gaattgctgc gcccgcagcg
    41161 gcgaaacggt gtaaaaacgc tttgctacct tcctccggcg tacagacaaa gaaatcgccc
    41221 tcggcctgtt tgaaataatc caccagccac gatttggctt caccgacgcc atcgcctgcc
    41281 atttttaaca gcagatgatg ctcgtattta tcgcgccagt ttttcatgcg cggcggtaaa
    41341 tggctgggga acaggtgacc gaatttttgc attgcacggt cagtaaaatg cggacggaag
    41401 aatttcactt tctccagcat cgcatcggtg cgtcccttga gattaaagaa gaacggcatc
    41461 ttgtcggtgc ctaacttatc aatcatcagg aaggtgtctt tgccgtattt ttccgcaata
    41521 tcgtagatat cccggtgcat atattccccg gcaaccggca gattttcgaa gttagccaga
    41581 atatgacggc ggatttcggt cagcacttcc ggctggttgg tgccgatata aaacacctgc
    41641 tgattttttt ccgcttcgaa ggtatcaaga cgtaccgcaa agacggccag cttcccggcg
    41701 caaccagaag attcaaataa ccgatccgga tcggcgttat aacgtgcggg cgtgtcggct
    41761 tcaatatcac gaacgcggtg gacatagtca taatcgtggg cgtgacgacc atcgtgacgc
    41821 acatcgtcat ctttgatgcg atcgtcatcc agcttgctaa ggatctgctc cggcgtttcg
    41881 cccagatcaa tccccagatg gttcaccagc gtcagtttgc catcttcatc tatacgcgcg
    41941 aataacgaca tttcggtata cgccgggcca cgttgcacca gcgagccgcc ggagttgtta
    42001 caaataccgc cgatgaccga tgcgcctata cacgatgatc caatcaccga atgcggttcg
    42061 cgtcccagcg gtttgagggc tttttccagc gaatagagcg tggtgcccgg ataggccagc
    42121 acctgttcgc ccttgccgag aacgtgcagc ttgtcgagac gcagggtgct gatgatcacg
    42181 atatcgcgat catagtcgtt accgtttggc gtcgatcctt cggtcaggcc tgtattggca
    42241 gcctgcatca gaataatttt gtcggcggtg acgcaggctt tcagcacccg ccacaattcc
    42301 agtagtgagc cagggaaaac gacagccagc gcgtcgccct gaccagaacg gaagcccttg
    42361 cgatagcggg ccgtttttgc gggatcggtg agcaggtgtg aatgaccgac cagacgggca
    42421 agttcattca aaaaggcttt attatcagtt gttgtcatgg aagacatttt ccactccttg
    42481 tggtggcgaa aaaatatcac aaactagaat agcgccttgt atgacaattc agcgagtgaa
    42541 tattttgaaa attcagacaa taaactgagc attctttttc tctctccatc atgcttatgg
    42601 caaactacgg tttttgcacc attttcgctg ccacgtcggg caacaaagga agaaaaatcc
    42661 atatgaaatg gctatgttca gtaggaatcg cggtgagtct ggtcctgcag ccagcactgg
    42721 cggatgattt attcggtaac catccattaa cgcctgaagc gcgggatgcg ttcgtcaccg
    42781 atttgctcaa gaaaatgacg gttgatgaga aaattggtca gctgcgctta atcagcgtag
    42841 gcccggataa tccgaaagag gcgatccgcg agatgatcaa agacggtcag gttggggcga
    42901 ttttcaacac cgtaacccgt caggatatcc gcgccatgca ggatcaggtg atggaattaa
    42961 gccgcctgaa aattcctctt ttctttgctt acgacgtgct gcacggtcag cgcacggtgt
    43021 tcccgattag cctcggtctg gcctcgtctt ttaacctcga tgcggtgaaa acggtcgggc
    43081 gtgtctctgc ttatgaagcg gcagatgatg gcctgaatat gacctgggca ccgatggtcg
    43141 atgtctcgcg cgatccgcgc tggggacgtg cttccgaagg ttttggcgaa gatacgtatc
    43201 tcacctcaat aatgggtaaa accatggtgg aagcgatgca gggtaaaagc ccggcagatc
    43261 gttactcggt gatgaccagc gtcaaacact ttgccgcata cggcgcggta gaaggcggta
    43321 aagagtacaa caccgtcgat atgagtccgc agcgcctgtt taatgattat atgccgccgt
    43381 acaaagcggg gctggacgca ggcagcggcg cggtgatggt ggcgctgaac tcgctgaacg
    43441 gcacgccagc cacctccgat tcctggctgc tgaaagatgt tctgcgcgac cagtggggct
    43501 ttaaaggcat caccgtttcc gatcacggtg caatcaaaga gctgattaaa catggcacgg
    43561 cggcagaccc ggaagatgcg gtgcgcgtgg cgctgaaatc cggaatcaac atgagtatga
    43621 gcgacgagta ctactcgaag tatctgcctg ggttgattaa atccggcaaa gtgaccatgg
    43681 cagagctgga cgatgctgcc cgccatgtac tgaacgttaa atatgatatg gggttgttta
    43741 acgacccata cagccatttg gggccgaaag agtctgaccc ggtggatacc aatgccgaaa
    43801 gccgcctgca ccgtaaagaa gcgcgtgaag tggcgcgcga aagcctggtg ttgctgaaaa
    43861 accgtctcga aacgttaccg ctgaaaaaat cggccaccat tgcggtggtt gggccactgg
    43921 cggacagtaa acgtgacgtg atgggcagct ggtccgcagc cggtgttgcc gatcaatccg
    43981 tgaccgtact gaccgggatt aaaaattccg tcggtgaaaa cggtaaagtg ctgtatgcca
    44041 aaggggcgaa cgttaccagt gacaaaggca ttatcgattt cctgaatcag tatgaagaag
    44101 cggtcaaagt cgatccgcgt tcgccgcaag agatgattga tgaagcggtg cagacggcga
    44161 aacaatctga tgtggtggtg gctgtagtcg gtgaagcaca ggggatggcg cacgaagcct
    44221 ccagccggac cgatatcact attccgcaag gccaacgtga cttgattgcg gcgctgaaag
    44281 ccaccggtaa accgctggtg ctggtgctga tgaacgggcg tccgctggcg ctggtgaaag
    44341 aagatcagca ggctgatgcg attctggaaa cctggtttgc ggggactgaa ggcggtaatg
    44401 caattgccga tgtattgttt ggcgattaca acccgtccgg caagctgcca atttccttcc
    44461 cgcgttctgt cgggcagatc ccggtgtact acagccatct gaacactggt cgtccgtata
    44521 atgccgacaa gccgaacaaa tacacttcgc gttattttga tgaagctaac ggagcgcttt
    44581 atccgttcgg ctatggtctg agctatacca ctttcaccgt ctctgatgtg aaactttctg
    44641 cgccgaccat gaagcgtgac ggcaaagtga cggccagcgt gcaggtgacg aacaccggta
    44701 agcgcgaggg ggccacggta gtgcagatgt acttgcagga tgtgacggct tccatgagtc
    44761 gccctgtgaa acagctgaaa ggctttgaga aaatcaccct gaaaccgggc gaaactcaga
    44821 ctgtcagctt cccgatcgat attgaggcgc tgaagttctg gaatcaacag atgaaatatg
    44881 acgccgagcc tggcaagttc aatgtattta tcggcactga ttccgcacgc gttaagaaag
    44941 gcgagtttga gttgctgtaa tgctttagta agggcgcgac gtttgcgccc tttgcctgat
    45001 gttcatatct taacttgctg aattttcgcc ctttgtaggc cggataaggc gctcgcgccg
    45061 catccggcag gaaacgccgg accgaatatg cctcttaagt atttatctga ttctgcaact
    45121 gaatccttcc gctcaggcta actctgtcat tatcaactat gcttttctct taattcgctg
    45181 aaaacaacag taaaagaatg aggaaagcat catgccactc ttaaagctct gggcaggttc
    45241 actggttatg ttggcagccg tgagcctgcc gctacaggcg gcttcccccg ttaaagtcgg
    45301 ttcaaaaatc gataccgaag gcgcgctgct cggcaacatc atctttcagg tactcgaaag
    45361 ccacggcgta ccgacggtga ataaagtgca actcggaacg actcctgtgg ggcgcggggc
    45421 gattacttcc ggtgaactgg atatctatcc ggaatatacc ggcaatggcg ctttcttctt
    45481 taaagatgaa aacgatgcag cgtggaaaaa tgccgggcag ggctacgaga aagtcaaaaa
    45541 gctcgatgcg gagcacaaca agttaatctg gctgacgccc gcgcctgcaa ataacacctg
    45601 gactatcgcc gtgcgtcagg atgtggcaga gaaaaacaaa ctcacttcgc ttgccgacct
    45661 gagtcgttat ctgcaagagg gcggcacctt caaactggca gcctcggcag agtttatcga
    45721 acgcgccgat gcgttaccca cgtttgaaaa agcctatggc tttaagctcg gtcaggatca
    45781 gttgctgtca ctggctggtg gcgacacggc ggtgacgatt aaagccgctg cccagcaaac
    45841 ctctggcgtt aatgccgcaa tggcttacgg cactgacggt ccggtcgcgg cgctggggct
    45901 gcaaacctta agcgatccgc aaggcgtgca acctatctac gcgcctgcac cagtggtgcg
    45961 tgaatcggtg ttgaaggagt atccgcaaat ggcacagtgg ctacagccag tcttcgccag
    46021 cctcgatgca aaaacattgc agcaactgaa tgccagcatt gcagtggaag gactggatgc
    46081 caaaaaagtg gctgccgact acctgaaaca aaaagggtgg gcgaagtaat ttcccgtgac
    46141 ttatctacgt attaatcctg ttctggcgct gctgttgttg ctgacggcaa tcgcagcggc
    46201 gctgccgttt atcagttacg cgcctaatcg tttagtctcg ggtgaagggc ttcatctctg
    46261 gcaactgtgg ccgcaaacga tctggatgct ggtgggcgtt ggttgcgcct ggctgacagc
    46321 ctgttttgtc cccgctaaaa aaggcagcat ttttgcactc attctggcgc aattcgtctt
    46381 cgtattgttg gtgtggggag ctggaaaggc ggcgacccaa ctggcgcaaa atggcagtgc
    46441 gctggcgcgt accagcctcg gcagaggttt ctggctggct gcggcgctgg cattgctggc
    46501 ctgtagcgat gccatccgcc gaatctccac gcatccgctg tggcgctggt tgttgcatat
    46561 gcagattgcc attattccgc tgtggttgct gtactccggc acgcttaacg atctctcact
    46621 aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac gcgctggcac aacatctgac
    46681 gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt gtgccgttgg gcatctggtg
    46741 ctacttttcc actgcgcggc agggggcaat tttttctctg ctcaatgtca ttcagaccgt
    46801 gccttcggtg gcgctctttg gcctgttgat tgcgccgctt gccgcgctgg ttacggcctt
    46861 tccgtggctg gggaagctcg gcatagcagg aaccggaatg acacccgcac tgattgcgct
    46921 ggtgctctat gccttgctgc cgctggtgcg aggcgtggta gtcggtttga accagatccc
    46981 gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc ggggcgcagc gtttcctgca
    47041 tgttcagtta ccgctggcgt tactggtatt tctgcgcagc ctgcgggtgg tgatggtgca
    47101 aactgtaggt atggcggtga ttgcggcgtt aatcggcgca ggcggttttg gtgcgctggt
    47161 tttccagggg ctgctaagca gcgccattga tttagtgttg ctgggggtga tcccggtaat
    47221 tgttctggcg gtgcttaccg acgcgctgtt cgatttgctt atcgcactgc tgaaggtgaa
    47281 acgtaatgat tgaatttagc catgtcagca aactgttcgg cgcacaaaaa gccgttaacg
    47341 atctcaatct caattttcag gaagggagtt tttcggtgct gattggcaca tctggctccg
    47401 gcaaatccac caccctgaaa atgattaacc gcctggtgga gcatgacagc ggagtgatcc
    47461 gctttgccgg agaagaaatt cgctcgctgc cagtactgga gttgcgccgc cggatgggct
    47521 atgccattca atctattggc ctgttccccc actggagcgt ggcgcaaaac atcgctaccg
    47581 tgccgcaatt acaaaaatgg tcgcgggcgc ggattgacga tcgtatcgac gaattaatgg
    47641 cgctactggg gctggagtca aatttgcgtg agcgttatcc gcatcagctt tccggtggtc
    47701 agcagcaacg tgtgggagtg gcgcgtgcac tggctgccga tccgcaagtc ttactgatgg
    47761 atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc gttgcaacaa gagatgacgc
    47821 gcattcaccg tttgctgggg cgcactattg tgctggtcac tcatgatatt gatgaggcgc
    47881 tacggctggc agaacatctg gtattgatgg atcacggtga agtggtgcag caggggaatc
    47941 cgctgacgat gctgactcgt ccggcgaatg attttgtccg ccagtttttt ggacgtagtg
    48001 aactgggggt gcacctgctt tcgttacgta gcgtggcgga ttacgtgcgt cgcgaagaac
    48061 gggcagaagg tgaggcactg gcagaagaga tgacgctacg cgatgcgctc tccctgtttg
    48121 tcgcgcgggg atgcgaggtg ctgccggtgg tgaacacgca gggccagcct tgcggcacgc
    48181 tgcattttca ggatctgctg gtggaggcgt aagcgtatga agatgttgcg cgatccgctg
    48241 ttctggctca ttgctctttt tgtggcgctg attttctggc tgccttacag ccagccgctg
    48301 tttgctgcct tgttcccaca actgccacga cccgtttatc agcaagaaag ttttgcagct
    48361 ctggcactgg ctcatttctg gctggtggga atttcgagtt tgtttgcggt gatcattggc
    48421 acaggtgccg gaattgctgt tactcgcccg tggggcgcgg aatttcgccc actggtggaa
    48481 actattgccg ccgttggaca gacttttccg cctgtcgcag tgctggcgat tgccgttccg
    48541 gtgatcggct ttggtctgca accagcgatt atcgccttga tcctttacgg tgtgctgccc
    48601 atcctgcagg tgacacttgc cgggctggga gcgattgatc ccagcgtgac agaagttgcg
    48661 aaaggtatgg gaatgagtcg aggtcagcga ctgcgtaagg tcgagctacc gctggcggct
    48721 ccggtgattc tggcgggcgt gcgaacttcg gtgattatca acattggtac ggcgacgatc
    48781 gcctcaacgg taggggccag tacgctgggt acgcccatca tcatcgggct tagcggattt
    48841 aataccgcgt atgtgatcca gggggcgtta ctggtggcac tggcggcgat catcgcagac
    48901 cgcctgtttg aaaggctggt gcaggcgctt agccagcacg caaaataaag gtataacctg
    48961 cgagcatgac gccaccaatt ccgcctaacg ccataaacag gaacagggcg atgaccccaa
    49021 ttttagctat gcgcataatg cactccttat gttaacgaaa ggattgtaca gtaaagcgca
    49081 tttgttaacg aatcattaaa taccgagtgg gaaaatatca tggccttgtt cttgccaact
    49141 ggtgagttgc tgctgttggg cggaggttcg attttcaccg caccacacca gcaatgtacg
    49201 gccttcgaat agttcagggc gtagttgatt gagcgagtgg gcgagcgagg acatcaatgc
    49261 gccatccttg ttgactggca atccagccct ccagccacag acgggtggta tcctgaatat
    49321 tccagccaac caccagcgca tctttaccct gttttttacg tgccgaagcc agacaaatgg
    49381 cgatgtagtt gatcagtacg ccgtcgagga tcgccagcag cgcctggaga gtcggttgtt
    49441 ggcactgaag ccgtcggcgc agaggaataa acagatgtgt ggtgagtgtc tgggcggggt
    49501 aatcctgacc gcgctctttg atccacgttc gcaggctatg tagattgcc
//
LOCUS       DAJTNW010000020        48569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNW010000020 DAJTNW010000000
VERSION     DAJTNW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1241
                     /locus_tag="OO179_001202"
     CDS             <1..1241
                     /locus_tag="OO179_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5531339.1"
                     /translation="ESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPD
                     DLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGN
                     ATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVK
                     PGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKG
                     IELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAG
                     LTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLD
                     AVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWN
                     EQAEKYLLAQFGVDIGEAAQ"
     gene            1238..2203
                     /gene="yggF"
                     /locus_tag="OO179_001203"
     CDS             1238..2203
                     /gene="yggF"
                     /locus_tag="OO179_001203"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5531340.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2225..2734
                     /gene="fumE"
                     /locus_tag="OO179_001204"
     CDS             2225..2734
                     /gene="fumE"
                     /locus_tag="OO179_001204"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5531341.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2731..3444
                     /locus_tag="OO179_001205"
     CDS             2731..3444
                     /locus_tag="OO179_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5531342.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3416..4098)
                     /locus_tag="OO179_001206"
                     /pseudo
     CDS             complement(3416..4098)
                     /locus_tag="OO179_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4092..4769)
                     /locus_tag="OO179_001207"
     CDS             complement(4092..4769)
                     /locus_tag="OO179_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5531343.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4757..5140)
                     /locus_tag="OO179_001208"
     CDS             complement(4757..5140)
                     /locus_tag="OO179_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5531344.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5156..5464)
                     /locus_tag="OO179_001209"
     CDS             complement(5156..5464)
                     /locus_tag="OO179_001209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531345.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5465..6043)
                     /locus_tag="OO179_001210"
     CDS             complement(5465..6043)
                     /locus_tag="OO179_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5531346.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6066..6497)
                     /locus_tag="OO179_001211"
                     /pseudo
     CDS             complement(6066..6497)
                     /locus_tag="OO179_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6869..7888
                     /gene="epd"
                     /locus_tag="OO179_001212"
     CDS             6869..7888
                     /gene="epd"
                     /locus_tag="OO179_001212"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5531347.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7938..9101
                     /gene="pgk"
                     /locus_tag="OO179_001213"
     CDS             7938..9101
                     /gene="pgk"
                     /locus_tag="OO179_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5531348.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9316..10395
                     /gene="fbaA"
                     /locus_tag="OO179_001214"
     CDS             9316..10395
                     /gene="fbaA"
                     /locus_tag="OO179_001214"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5531349.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10586..11446
                     /gene="mscS"
                     /locus_tag="OO179_001215"
     CDS             10586..11446
                     /gene="mscS"
                     /locus_tag="OO179_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5531350.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11585..12220
                     /gene="argO"
                     /locus_tag="OO179_001216"
     CDS             11585..12220
                     /gene="argO"
                     /locus_tag="OO179_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5531351.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12313..13053
                     /locus_tag="OO179_001217"
     CDS             12313..13053
                     /locus_tag="OO179_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5531352.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13220..14170
                     /locus_tag="OO179_001218"
     CDS             13220..14170
                     /locus_tag="OO179_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5531353.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVAGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14112..15590)
                     /gene="scpC"
                     /locus_tag="OO179_001219"
     CDS             complement(14112..15590)
                     /gene="scpC"
                     /locus_tag="OO179_001219"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5531354.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15614..16390)
                     /gene="scpB"
                     /locus_tag="OO179_001220"
     CDS             complement(15614..16390)
                     /gene="scpB"
                     /locus_tag="OO179_001220"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5531355.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16401..17396)
                     /gene="meaB"
                     /locus_tag="OO179_001221"
     CDS             complement(16401..17396)
                     /gene="meaB"
                     /locus_tag="OO179_001221"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5531356.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17389..19533)
                     /gene="scpA"
                     /locus_tag="OO179_001222"
     CDS             complement(17389..19533)
                     /gene="scpA"
                     /locus_tag="OO179_001222"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5531357.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19737..20630)
                     /gene="argP"
                     /locus_tag="OO179_001223"
     CDS             complement(19737..20630)
                     /gene="argP"
                     /locus_tag="OO179_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5531358.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21013..21672
                     /gene="rpiA"
                     /locus_tag="OO179_001224"
     CDS             21013..21672
                     /gene="rpiA"
                     /locus_tag="OO179_001224"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5531359.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21928..23160
                     /gene="serA"
                     /locus_tag="OO179_001225"
     CDS             21928..23160
                     /gene="serA"
                     /locus_tag="OO179_001225"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5531360.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23389..23448
                     /gene="ibsC"
                     /locus_tag="OO179_001226"
     CDS             23389..23448
                     /gene="ibsC"
                     /locus_tag="OO179_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5531361.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23781..23840
                     /locus_tag="OO179_001227"
     CDS             23781..23840
                     /locus_tag="OO179_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5531362.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23941..24543)
                     /locus_tag="OO179_001228"
     CDS             complement(23941..24543)
                     /locus_tag="OO179_001228"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5531363.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24564..24747)
                     /gene="ssrS"
                     /locus_tag="OO179_001229"
     ncRNA           complement(24564..24747)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO179_001229"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24789..25118)
                     /gene="zapA"
                     /locus_tag="OO179_001230"
     CDS             complement(24789..25118)
                     /gene="zapA"
                     /locus_tag="OO179_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5531364.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25286..25864
                     /gene="ygfB"
                     /locus_tag="OO179_001231"
     CDS             25286..25864
                     /gene="ygfB"
                     /locus_tag="OO179_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5531365.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25890..27215
                     /gene="pepP"
                     /locus_tag="OO179_001232"
     CDS             25890..27215
                     /gene="pepP"
                     /locus_tag="OO179_001232"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5531366.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27212..28390
                     /gene="ubiH"
                     /locus_tag="OO179_001233"
                     /gene_synonym="visB"
     CDS             27212..28390
                     /gene="ubiH"
                     /locus_tag="OO179_001233"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5531367.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28413..29615
                     /gene="ubiI"
                     /locus_tag="OO179_001234"
                     /gene_synonym="visC"
     CDS             28413..29615
                     /gene="ubiI"
                     /locus_tag="OO179_001234"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5531368.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29689..29772)
                     /locus_tag="OO179_001235"
     CDS             complement(29689..29772)
                     /locus_tag="OO179_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531369.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30063..31157
                     /gene="gcvT"
                     /locus_tag="OO179_001236"
     CDS             30063..31157
                     /gene="gcvT"
                     /locus_tag="OO179_001236"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5531370.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31181..31570
                     /gene="gcvH"
                     /locus_tag="OO179_001237"
     CDS             31181..31570
                     /gene="gcvH"
                     /locus_tag="OO179_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5531371.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31689..34562
                     /gene="gcvP"
                     /locus_tag="OO179_001238"
     CDS             31689..34562
                     /gene="gcvP"
                     /locus_tag="OO179_001238"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5531372.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34829..35571
                     /locus_tag="OO179_001239"
                     /pseudo
     CDS             34829..35571
                     /locus_tag="OO179_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35628..37061)
                     /gene="bglA"
                     /locus_tag="OO179_001240"
     CDS             complement(35628..37061)
                     /gene="bglA"
                     /locus_tag="OO179_001240"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5531373.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLLGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37106..37416
                     /locus_tag="OO179_001241"
                     /pseudo
     CDS             37106..37416
                     /locus_tag="OO179_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37580..38239
                     /locus_tag="OO179_001242"
     CDS             37580..38239
                     /locus_tag="OO179_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5531374.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38435..39415)
                     /gene="ygfZ"
                     /locus_tag="OO179_001243"
     CDS             complement(38435..39415)
                     /gene="ygfZ"
                     /locus_tag="OO179_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5531375.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39658..39924
                     /gene="sdhE"
                     /locus_tag="OO179_001244"
     CDS             39658..39924
                     /gene="sdhE"
                     /locus_tag="OO179_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5531376.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39905..40312
                     /locus_tag="OO179_001245"
     CDS             39905..40312
                     /locus_tag="OO179_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5531377.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40352..40873)
                     /gene="fldB"
                     /locus_tag="OO179_001246"
     CDS             complement(40352..40873)
                     /gene="fldB"
                     /locus_tag="OO179_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5531378.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40985..41881
                     /gene="xerD"
                     /locus_tag="OO179_001247"
     CDS             40985..41881
                     /gene="xerD"
                     /locus_tag="OO179_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5531379.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41906..42616
                     /gene="dsbC"
                     /locus_tag="OO179_001248"
     CDS             41906..42616
                     /gene="dsbC"
                     /locus_tag="OO179_001248"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5531380.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42622..44355
                     /gene="recJ"
                     /locus_tag="OO179_001249"
     CDS             42622..44355
                     /gene="recJ"
                     /locus_tag="OO179_001249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5531381.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44446..45544
                     /gene="prfB"
                     /locus_tag="OO179_001250"
     CDS             join(44446..44520,44522..45544)
                     /gene="prfB"
                     /locus_tag="OO179_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5531382.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45554..47071
                     /gene="lysS"
                     /locus_tag="OO179_001251"
     CDS             45554..47071
                     /gene="lysS"
                     /locus_tag="OO179_001251"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5531383.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47114..47662)
                     /gene="idi"
                     /locus_tag="OO179_001252"
     CDS             complement(47114..47662)
                     /gene="idi"
                     /locus_tag="OO179_001252"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5531384.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47717..47788
                     /locus_tag="OO179_001253"
     CDS             47717..47788
                     /locus_tag="OO179_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531385.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47785..47910)
                     /locus_tag="OO179_001254"
     CDS             complement(47785..47910)
                     /locus_tag="OO179_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531386.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47912..>48569)
                     /gene="uacT"
                     /locus_tag="OO179_001255"
     CDS             complement(47912..>48569)
                     /gene="uacT"
                     /locus_tag="OO179_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5531387.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11650 a        12180 c        13114 g        11625 t
ORIGIN      
        1 tggagtcatt acccgaattt gaactgccag aaattaccga taatgaactg ttagtgagtg
       61 taatttctga cagcgtctgt ttatcgacct ggaaagcggc gttactcggt agtgaacata
      121 aacgcgtacc cgacgattta gaaaatcatc cggtcattac cgggcatgaa tgtgccgggg
      181 ttattgtcga agtgggtaaa aatctcactg gcaaatataa aaaaggccag cgttttgtat
      241 tgcaaccggc gatggggtta ccaagcggat attcagcggg ctacagctac gaatattttg
      301 gcggcaatgc cacttatatg attattcccg aaatcgccat taatttgggc tgcgtattac
      361 cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga gcctatgtgc tgcattattg
      421 gtgcttatca tgccaattat cacaccacgc aatatgttta tgagcatcgc atgggcgtca
      481 aacctggcgg caatattgca ctgctggcgt gtgcgggtcc gatgggcatt ggcgctatcg
      541 attacgccat taacggtggc atacaaccgt cacgggtggt ggtggtcgat atcgacgaca
      601 aacgcctggc gcaggtgcag aagctgctgc cggtggatct ggcggccagt aaaggcattg
      661 agctagtgta tgtgaatacc aaagggatga gcgatcctat ccagatgctg cgggcgctga
      721 caggagatgt cgggttcgat gacatttttg tttatgcggc ggtgcctgct gtcgttgaga
      781 tggctgacga attactggcg gaagatggct gtctgaactt ctttgccggg ctgacggata
      841 aaaacttcaa agtgccgttt aatttctaca acgtccatta caacagcacg cacgtagtcg
      901 gtacatccgg cggttcaacg gacgacatga aagaggcgat tgcccttagc gccacaggac
      961 agttacagcc gtcgtttatg gtgacccata ttggtggcct ggatgcggtg ccagaaaccg
     1021 tgctcaatct gccggatatc cctggcggta aaaaactcat ttataacggc gtgaccatgc
     1081 cgctcactgc cattgccgat tttgccgaaa aaggcaaaac cgatccgctg tttaaagagt
     1141 tggcgcggct ggttgaggaa acgcacggca tctggaatga acaggccgag aaatatctgc
     1201 tggcacaatt tggcgttgat atcggggagg ccgcgcaatg atgtccctgg cgtggccatt
     1261 atttcgcgtt acggaacagg cagcgttggc tgcctggccg cacaccggat gtggcgacaa
     1321 aaataaaatt gatggcctgg cggtcaccgc gatgcgccag gcattaaacg acgtcgcttt
     1381 tcgtggacga gtggttatcg gcgaaggaga aattgaccat gcgccgatgc tgtggattgg
     1441 cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc gcggttgatc ccattgaagg
     1501 cacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg gtcatggctt tcgctcctcg
     1561 agatagcctg ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta atcgactggc
     1621 tgccggggcg attgatctgt ctttgcctct ggcggacaac ttgcgtaacg tggcgcgggc
     1681 gttaggtaaa ccgctggaca agctgcgcat ggttacgctg gataaaccgc gtctgagtgc
     1741 cgcgattgaa gaagcaacac agctgggcgt gaaggttttt gccctgccgg atggcgatgt
     1801 cgccgccagc gtgctgacct gctggcagga taatccttac gatgtgatgt acaccatcgg
     1861 cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa gcgttaggcg gggatatgca
     1921 ggccgagctg attgactttt gtcaggctaa aggggattac acggaaaatc ggcagattgc
     1981 agagcaggag cgcaagcgtt gtaaggcaat gggcgttgac gttaaccgtg tgtactcgct
     2041 cgatgaactg gtgaggggga acgatatcct ctttagcgct acgggcgtga cgggcggcga
     2101 gttggtgaac ggtatccaac agacggcgaa tggggtgcgg acgcagacat tactgatcgg
     2161 cggcgcggac caaacgtgta atataataga ctccctgcat tgatggtgat ctatcggaag
     2221 accaatggcg acgctgacag aagatgatgt gcttgagcaa ctggatgcac aggacaattt
     2281 attttcattt atgaaaactg cgcattctat tttgctccag gggatacgcc agtttctgcc
     2341 gtcgctgttt gtcgataacg atgaagagat cgtcgaatat gcagtgaagc cgttactcgc
     2401 ccaaagcggc ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg cgctggggaa
     2461 aatggataaa tggctgtatg ccgatatcac gcacttttcc cagtactggc attacctgaa
     2521 cgaacaggat gaaacgcccg gatttgccga tgatataacc tgggatttta tcagcaatgt
     2581 caacagtata actcgcaatg cgacgctcta cgatgcgtta aaagcgatga agttcgccga
     2641 tttcgccgtc tggtcagagg cgcgttttag cggaatggtc aaaacggcgc tgacgctggc
     2701 agtaacgacc accttaaagg aattaacgcc gtgaaaattg aattaacggt gaatgggctg
     2761 aacgtgcaag cgcagtacca tgatgaagag atagaacgcg tgcataaact gttgctgcgt
     2821 atgctggcgg ccttacagac ggtaaatccg cagcggcgta cggtggtttt tctttgcgct
     2881 ccgccgggga cgggtaaatc taccctaacc accttctggg aatatctcgc gcaacaagac
     2941 ccggagctgc ccgctattca ggcgctcccg atggatggtt ttcaccatta caatagctgg
     3001 ctggacgcgc atcaattgcg ccccttcaaa ggcgcaccag agacatttga cgttgcgaaa
     3061 ctggcggaaa atctgcgcca ggtggtggaa ggggattgta cgtggccgca gtacgatcga
     3121 caaaagcatg atcctgttga agatgcgttg cacgttaccg caccactcgt catcgtcgaa
     3181 ggaaactggt tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt ttgtgatttc
     3241 tcgatcttta tcaacgcgcc agctgctgcg ttgcgggagc gtctggtggg gcgcaagctg
     3301 gcgggagggg tatcgctggc agatgcagaa gccttttatg accgtaccga cgggccgaat
     3361 gttcgccggg tgctggaaga gagtctgcct gcaaatttga ccttaatgat gaccgctacg
     3421 ggtgaatatc gtctggtgga ttaacgttcc tgattacacc atcttccagc cgcaccacac
     3481 gagagaaatg acgccgggta aatgcggcgt catgactgat tgctaccacg ctcgtgcctc
     3541 gctggcggca cttctccagc cagctttcaa acacgctcaa ccaattttcg tcaaaatccc
     3601 gactcggctc gtccagcagt aatagtggcg gagagagtgc ttccaggcag gcgacggcga
     3661 ccatccgtcg ctgcgcacta tgcagatcca acgggtgcga gttggcagtg tctgctaact
     3721 gacaacattg cagggcagca tgggtacgtt gagtgatttc atccgccggg catttttgca
     3781 gtttcaaacc aaatgcgatc tcgtcagcga cggtgctgtg aaaaagttgg ttttccgctt
     3841 cctgaaacag aacagtacgc caacctttgc cgcgcgctgg cggtttttca ggtttttcat
     3901 cgcctgttgt tgcagcataa ccgtaccggc tgtgggcgta agaagacctg ccattactcg
     3961 taacagcgtc gatttccctg cgccattgtc accggtcagc gccaaccatt caccttgttt
     4021 gagttgcaga gaaatgtcgc aaagacaatc cgttgccgcg ccaggccagc gataagatat
     4081 cttatttaac gttaacatag tggctggagc gctccgtttt gcaattgcca cgcttgctgg
     4141 cagtagttca ggaaaggtgt gcgatgacgt tcaaaaagaa taatcagcga accacgttca
     4201 aacgcccagt gttgtaagcg ctgcaacagc atttcgctgg cctgcggcgt caggcggcta
     4261 aaggcttcat caagaattaa gagctttggc tgcatcgcga tggcgcaggc gatgatcacg
     4321 cgctgtgtct ctccaccgga gagtgttgcc gggtgacgat ggcgcaacgg ctggcactcg
     4381 gtcagagtca gggcggcatc aatccgcgcc ataatctctg cttcagcaag acacagattt
     4441 tctggcccaa aggcaacttc ttcttccacg ctaaaggtac agccggagag ctgcaaatag
     4501 ggcgattgct ggacgagctg gatggtggcg gattgttcat taagcgggag atgcccaatg
     4561 ggcgtaccga ggagcgtccc cgtgcctgtt atctctcttg gcagaaagtc cggataccag
     4621 cccgccatca gctgtgccag cgtacttttg ccactgccgt tgtcaccaaa gatggcgacc
     4681 atccccggtt tgacatagtg aaagtcataa cagaacggag gatgcgttga gtgtgtcggg
     4741 caataacgga actgttctaa cgtaaccata tccacgctcc gaattctgtc agcatcagca
     4801 agatcatggt gtaacgcgct acgcgttgta gggtgctatc ggctggtgcc cacaacgttg
     4861 ttcgtcggtt gtggatccga aatgcccgca tatccagcgc cgcaccacgg acagccagat
     4921 cgttaagcgc attgtgtgtt agcggaataa ttaacgcggg catagcacgt aaacgctgat
     4981 accagccctc atccagcggc acgccgcggg cacgctgcgc ttcatgaata atcgctaatt
     5041 gccgttttaa ctgctcaacg accaataacg gcccggcaaa cagataagcg acgcctggcg
     5101 gcaggcgaga agcaaacaga gcgcgaataa aacattgcac ggggacgtac tgcattcaca
     5161 actgtgaggt tgaaacaatc gccagaatca gcaaccagag cgtaatagca tgcgaccacc
     5221 gttctggcga gcgtggcgtg ccgcttagcc attctggtag ccagccaccg tgtaccagcc
     5281 acagcccggc tcccagcgag aacatcagcc agacgacata ttttgcccgc cgccgcgtgg
     5341 cctttagcgc aataaggcag aaaaaggtcg ccgcgctata gatcggcaaa atggtctgcg
     5401 cgggcaggat aagcgtggtg caggccgcca atgcccacag tgttaatgag gtaaatggat
     5461 gcatttagcg cacggcagcc attgccggaa aatggcgcgt ggtgcgaatc ggcagctggc
     5521 gcagtaaaag ccagacaatc accgctgtaa gaattttatc gaccaggttc gcgcctatca
     5581 ccgtaatcgc caccgactcc accaggtttt gccccatcga gtgcatccag gcaacgaaca
     5641 aatcggctcc gctaccggtg acaccgccga acagcgcagt acgcagcggt acagcgacga
     5701 ccgttaccgc aagcgtaata atcacgccgc tgacaacgac ttttggcagg gtgcgaaacc
     5761 aacccgctcg cgccagccaa ccggagacca ggccgatgac catcgcgacg ggtgcaaatg
     5821 cggcggcgat cgggtcggtc agcaatcccc agagtaaatt ggtcagtaag ccggtcagca
     5881 ttccgatcac cggaccaagc agtaccgcgc taataagtgt gccgatggag tcgagaaata
     5941 taggtaattt gaccatgctg gccagttgcc cgccgatcat attgatggcg atagatataa
     6001 cgatcaacac cagtgcctga ctggaaaagt ggctacgcgc cataagaaaa cctctttgaa
     6061 agtagttatt taacggggga aatatgcaac tcgtcgtaac cagaaagcgc gcaaggttgg
     6121 cggctaaacg ccagttcctc aaggctaccg atcaccagtt caagcgtggt ccgcacggta
     6181 cagccgggca tcatatgacc gccaagcatc gtgccgtgtg gatcggaaac gcagagatga
     6241 agatgctcac cactctgttc cagcgtgccg tttagtgaga tcacttcgaa tgtaccgttc
     6301 aggagcgtgg tgccttcttg cccggcatag cgcaaggcga cgtcagtcag gctaccggta
     6361 cagcctgcaa tctaggcggc atgaagttga tgttgctgtg caaaagcgtg tagctgggaa
     6421 aaaacttcct gcccggggag gaggcgcagt gcgtaaaagc gtgcggagga agaatatgag
     6481 tagggcaatg tcatcattgg agtactccga aactcggacg tattgcggat aatatcgacg
     6541 ctgcatcagt catattctga tataagtcag ggtttgtgac ggcacaacga aaagattttg
     6601 ctacgcttat tcgtgattct gcaaagtgga ataaattcca caggggcggg gctgaaaacg
     6661 tgacgtaagt cagataattg ctttctttga ctggacaatc attcctttta ttccacgttt
     6721 cgcttatcct agctgaagcg tttcagtcga ttaaatgttc gacaattaac caatcagtcg
     6781 cagtttgcga caggtaaggt ttccccggac gatttgctgg attactctgt ctggcaaatt
     6841 tgctcaggga aaaccttgca ggagatctat gaccgtacgc gtagcgataa atggcttcgg
     6901 tcgcatcggg cgtaatgtgg ttcgtgcttt gtatgaatcc ggacgccggg cggaaattac
     6961 cgtggtggca atcaacgaac tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga
     7021 caccagccat ggccgttttg catgggaagt acgacaggaa cgcgatcaac tttttgttgg
     7081 tgatgacgcc atccgcgtat tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact
     7141 tggcgttgat gtagtcctcg actgcaccgg cgtatatggc tcccgcgagc atggcgaagc
     7201 gcatattgcc gccggggcca aaaaagtgct cttttcacat cctggcagta acgatctcga
     7261 cgcgaccgtt gtttacggcg tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc
     7321 taacgcttcc tgtaccacga attgcataat tcccgtcatc aaattgttag atgatgcgta
     7381 cggtattgag tccggcactg tgaccacaat tcactccgcc atgcacgatc aacaggttat
     7441 tgatgcatac catcctgacc tgcgtcgcac ccgggcagcc agccagtcga tcattccggt
     7501 cgatactaaa ctggccgctg gtatcacacg attttttccg caatttaacg atcgctttga
     7561 agcgattgcg gtacgtgtgc caaccataaa tgtgacggca atcgatttaa gcgtgacggt
     7621 gaagaaacct gtaaaagcca atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc
     7681 atttcatggt atagttgact atacggaatt gccgttggtc tctgtagatt ttaaccacga
     7741 tccgcacagt gccattgtcg atggcaccca aacccgggtc agtggcgcac acctgatcaa
     7801 aacgttggtc tggtgcgata acgaatgggg ctttgctaac cgaatgctcg acacgacgtt
     7861 agctatggct actgttgctt tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat
     7921 caacgagagg attcaccatg tctgtaatta agatgaccga tctggatctt gctgggaaac
     7981 gtgtatttat ccgtgcggat ctgaacgtac cagtaaaaga cgggaaagta accagcgacg
     8041 cgcgtatccg tgcttctctg ccgaccatcg aactggccct gaaacaaggc gcaaaagtga
     8101 tggtaacttc ccacctgggt cgtcctaccg aaggcgagta caacgaagaa ttctctctgc
     8161 tgccggttgt taactacctg aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt
     8221 acctcgacgg cgttgacgtt gctgaaggtg aactggttgt tctggaaaac gttcgcttca
     8281 acaaaggcga gaagaaagac gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg
     8341 tgttcgtaat ggacgcattc ggtactgctc accgcgcgca ggcttctact cacggtatcg
     8401 gtaaattcgc tgacgttgcg tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg
     8461 gtaaagcact gaaagaacct gctcgcccga tggtggctat cgttggtggt tctaaagtat
     8521 ctaccaaact gaccgttctg gactccctgt ctaaaatcgc tgaccagctg attgttggtg
     8581 gtggtatcgc taacaccttt atcgcggcac aaggccacga tgtgggtaaa tccctgtacg
     8641 aagctgacct ggttgacgaa gctaaacgtc tgctgaccac ctgcaacatc ccggttccgt
     8701 ctgatgttcg cgtagcaacc gagttctctg aaactgcacc ggctaccctg aaatctgtta
     8761 acgatgtgaa agctgacgag cagatcctgg atatcggtga tgcttccgct caggaactgg
     8821 ctgaaatcct gaagaatgcg aaaaccattc tgtggaacgg tccggttggc gtgttcgaat
     8881 tcccgaactt ccgcaaaggt actgaaatcg tggctaacgc tatcgcagac agcgaagcgt
     8941 tctccatcgc tggcggcggc gacactctgg cagcaatcga cctgttcggc attgctgaca
     9001 aaatctccta catctccact ggcggcggcg cattcctcga attcgtggaa ggtaaagtac
     9061 tgcctgcagt agcgatgctc gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa
     9121 acctgcactt gtttcagcgc gcttttagag cacgcatttt ttcatactct aaataattcg
     9181 agttgcagga aggcgacaag agagtgaatc cccaggagct tacataagta agtgactggg
     9241 gggagcgaac gcagacgcag cacatgcaac ttgaagtatt atgagtataa ggcccgacga
     9301 tacaggacaa gagacatgtc taagattttt gatttcgtaa aacctggcgt aatcactggt
     9361 gatgacgtac agaaagtttt ccaggtagca aaagaaaaca acttcgcact gccagcagta
     9421 aactgcgtcg gtactgactc catcaacgcc gtactggaaa ctgctgctaa agttaaagcg
     9481 ccggttatcg ttcagttctc caacggtggt gcttccttta tcgctggtaa aggcgtgaaa
     9541 tctgacgttc cgcagggtgc tgctatcctg ggcgcgatct ctggtgcgca tcacgttcac
     9601 cagatggctg aacattatgg tgttccggtt atcctgcaca ctgaccactg cgcgaagaaa
     9661 ctgctgccgt ggatcgacgg tctgctggac gcgggtgaaa aacacttcgc agctaccggt
     9721 aagccgctgt tctcttctca catgatcgac ctgtctgaag aatctctgca agagaacatc
     9781 gaaatctgct ctaaatacct ggagcgtatg tccaaaatcg gcatgactct ggaaatcgaa
     9841 ctgggttgca ccggtggtga agaagacggc gtggacaaca gccacatgga cgcttctgca
     9901 ctgtacaccc agccggaaga cgttgattac gcatacaccg agctgagcaa aatcagcccg
     9961 cgtttcacca tcgctgcgtc cttcggtaac gtacacggtg tttacaagcc gggtaacgtg
    10021 gttctgactc cgaccatcct gcgtgattct caggaatatg tttctaagaa acacaacctg
    10081 ccgcacaaca gcctgaactt cgtattccac ggtggttccg gttctactgc tcaggaaatc
    10141 aaagactccg taagctacgg cgtagtgaaa atgaacatcg ataccgatac ccaatgggca
    10201 acctgggaag gcgttctgaa ctactacaaa gcgaacgaag cttatctgca gggtcagctg
    10261 ggtaacccga aaggcgaaga tcagccgaac aagaaatact acgatccgcg cgtatggctg
    10321 cgtgccggtc agacttcgat gatcgctcgt ctggaaaaag cattccagga actgaacgcg
    10381 atcgacgttc tgtaagtcat tcctttctgc ttatctcaag gcccgctctg cgggtctttt
    10441 tttcgccaaa agcaaggtga ttcagatgag aaatctgtga tctatttggc aaaattatgc
    10501 tttattgttt acccttgtca gactgcccgt cataaggcgg cggagtgtat ttctccattt
    10561 tgagtcagtt gaaaaggaat attgaatgga agatttgaat gttgtcgata gcataaacgg
    10621 cgcgggaagc tggctggtag ctaaccaggc gctgctgcta agttatgcag taaacatcgt
    10681 ggcggcactc gcgatcatca tcgttggttt gattatcgcg cggatgattt ccaacgcggt
    10741 gaatcgcctg atgatctccc gtaaaatcga tgccactgtt gctgattttc tttctgcatt
    10801 agtccgttac ggtattatcg cctttacgct aatcgctgcc ctgggacgcg tgggtgtaca
    10861 aacagcgtca gtcattgctg tactcggtgc cgcaggctta gctgttggtc tggctttgca
    10921 ggggtcactt tctaacctgg ccgctggcgt gttacttgtc atgttccgcc cgttccgtgc
    10981 cggagaatat gttgacctcg gcggcgtagc cggtactgtg ctgagtgtgc agattttctc
    11041 caccaccatg cgtactgcag acggtaaaat tatcgttatt ccgaacggta aaattattgc
    11101 cggaaatatt attaacttct cccgcgagcc agttcgccgt aacgaattta ttattggcgt
    11161 ggcgtatgat tccgatatcg atcaggttaa gcagatcctg accaatatta tccagtctga
    11221 agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg aacgaacttg gtgcatcgtc
    11281 gattaatttc gtggtccgcg tctggagcaa cagcggcgat ctgcaaaacg tgtactggga
    11341 tgtgctggag cgtattaaac gtgaatttga tgccgccggt atcagcttcc cgtacccgca
    11401 aatggatgtg aactttaagc gggtgaaaga agacaaagct gcgtaatcaa cgctgaggcc
    11461 agataatact ctctggcctc tctcttatta gttattctga ttgccaatta atattatcaa
    11521 tttccgctaa taacaatccc gcgatatagt ctctgcatca gatacttaat tcggaatatc
    11581 caacgtgttt tcttattact ttcaaggtct tgctcttggg gcagctatga ttctgccgct
    11641 cggcccacaa aatgcttttg tgatgaatca gggcattcgt cgtcagtacc acattatgat
    11701 tgccttactt tgcgctatca gcgatttggt cctgatttgc gcggggattt ttggtggtag
    11761 cgcgttattg atgcagtcgc cgtggttgct agcgctggtc acctggggcg gcgtagtctt
    11821 cttgctctgg tatggttttg gcgcttttaa aacagcgatg agtagtaata ttgagttagc
    11881 tagtgccgaa gtcctgaagc aaggcagatg gaaaattatc gccaccatgt tggcagtgac
    11941 ctggctgaat ccgcatgttt acctggatac ttttgttgta ctgggcagcc ttggcgggca
    12001 gcttgacgtc gaaccaaaac gttggtttgc gctcgggaca attagcgcct ctttcctgtg
    12061 gttctttggt ctggctattc tcgcagcctg gctggcaccg cgcctgcgca cggcaaaatc
    12121 acagcgcatt atcaatctgg tagtgggatg tgtcatgtgg tttatagcct tgcagctggc
    12181 gagagacggt attgctcatg cacaagcctt gttcagttag gcgctatctg atggaaaaat
    12241 aaaacagagg cgctaagctt gcctccagag gtcctgaatt ttgggcaagt caattaacac
    12301 ggagagacta acgtgaagtt caaagttatc gccctggcgg cattaatggg tattagcggg
    12361 atggcagcgc aggctaacga attgccggat ggaccgcata ttgtcacctc cggtacggca
    12421 agtgtggatg cggtgccaga cattgccact cttgcgattg aagttaacgt ggccgcgaag
    12481 gatgccgcta cagccaagaa acaggcagat gagcgcgtcg cacaatacat ttccttcctt
    12541 gaactcaatc agatcgcgaa aaaagatatc agctcagcga atttacgcac ccagccagat
    12601 tatgactatc aggatggtaa aagtatcctg aaaggctacc gagctgtgag aacggtggaa
    12661 gtcacgctcc gtcagttaga caaactgaat tccttgctgg atggcgcgct gaaggcgggt
    12721 cttaacgaaa ttcgttctgt gtcgctgggc gtggcgcagc cggatgccta taaagacaaa
    12781 gcgcgtaagg cagcgattga taacgcgatt catcaggcgc aggaactggc gaacggcttt
    12841 catcgtaaac tggggccggt atatagcgtg cgctaccatg tttccaacta tcagcccagc
    12901 ccaatggtgc ggatgatgaa agccgatgcc gcgccggtgt ctgcccagga aacttacgag
    12961 caggccgcta ttcagtttga tgatcaggtc gatgtggtct tccagttaga acctgtggat
    13021 caacaacccg ctaaaacacc tgcagcacaa taatacttgt agcccgacgt attcgtcggg
    13081 ctaatgcctt tacccgatat tgcctccttt ttctgtaatt ttctgtaaaa ccatgacgta
    13141 atcacctatc tttgctcgcg gtttcatcaa ggataggctg actgaaacgg ttaagcggac
    13201 aaggatgcgg ggagggggga tggacatttt tatttcgaaa aagatgcgca atttcatttt
    13261 attagcgcaa accaataata ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc
    13321 atttggtaaa agcattgccg cgctggaaga acaaattggc tatacgctat ttacccgcaa
    13381 agataataat atcagcctca ataaagccgg tcaggaactg tatcaaaaac tgttcccggt
    13441 ttatcaacga ctttctgcga ttgataatga aatccataac tcagggcgtc gttcacgaga
    13501 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
    13561 cggagataag tatgaaggcg tgactgccca gccagttgag ttcagcgaaa acggcgttat
    13621 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
    13681 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgggctttct
    13741 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctggct
    13801 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
    13861 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
    13921 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
    13981 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
    14041 ttcaatcaga aatcatcgtt ataccgccac gatcttcagc atattgctga agatcgtggc
    14101 gggacaaacc attaacccag catcgagccg gttgcaatta aattacggtg taagtcgaag
    14161 acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat tttccagata gcgatgcaga
    14221 taatcccgat acataggatg tgcacaatta tcaatgatag tgcgggcgcg ttgaagcggg
    14281 gaaagaccgc gcagatcggc gatcccttgt tcggtgatga tcactttgac gctgtgttcg
    14341 ctgtgatcaa catggctgca cattggcacg acggttgaga tcttgccttc tttagcaatc
    14401 gacggagcca taaagatcga cagatacgcg ttgcgttcaa aatcaccgct gccgccgatg
    14461 ccgttcatca gatcgacccc ggctacgtgc gttgaatttg catgcccgta aatatcaaac
    14521 tccaggccga cgttcagagc gatgacgccc agacgacgga tgatttccgg gttattggaa
    14581 atctcctgcg gacgcaacac aatgcggctg gcaaagtaat ccatattgtc gtaaatcttg
    14641 cgcagggaat cggccgagat tgtcaggctg gaggcgctgg ccccgctgat tttgccggtt
    14701 tccagtaaat gcaccaccga ttcctgtagc acttccgaat acatcatgaa cggaggaatt
    14761 tccgggtttt cccccagacg tgccattacc gcattattga tattgcccac gccactttgc
    14821 agcggcagaa attccggcgg aatacgccca tgcgccattt cctgcaataa gaacgtgacc
    14881 acgttatcgg caatctgctg gcacatggga ttttgcttat ccagcatatt accggcgtcg
    14941 ggcaagttgg tttccacgac ggcgacaatc tttttcggat cgatttgcac atagcgggta
    15001 ccgacgcgat ccattgcatg gaagatcgac acgctattgc gccgcggtgg cgcgccagga
    15061 atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc
    15121 actttcttcg ctcgcagcag ccaggtcggc gcattaccga tcccgctggt taaccagact
    15181 cgaccatccg gtgccagtgc cgatgcttca atgacggcaa catcaatgtc gccgaagaaa
    15241 ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt caacgaaact caccgcgccc
    15301 tgattgatct ttttacgtaa actggacgat gtttgatatg gcgcacgcca ggaaacagca
    15361 tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg acgcccccgt cagcaggcga
    15421 atttgatacg gctttttggc ctcatgctgt tcgttagctc tgcgggcaat cgcggcgggt
    15481 agggctttcg gcgaacccgc tggggtaaag ccgctaaatg ccaccatgtc gttatgctgg
    15541 ataatttctg ccgcttcatc ggcggtcatc cttgtccact gagtttccat ttttactcct
    15601 tcgttcgcag ggattaatga ccaacgaaat taggtttacg tttttcgagg aaagcgttca
    15661 tcccttcctg gtaatcttcg ctgtcataaa ccgcgcggcg catcccctga atacgttcaa
    15721 attcatcgga gttcatggtg tgtgcttcgc ccagtacacg cagctcttct ttgataacgg
    15781 caatggctaa cggcgctttc tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca
    15841 cttccacaac atggttgagg atgccgacag ccagcgcgcg ctgggcggtg attggcgaag
    15901 cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg ttgtgaatgc
    15961 cgaccaggtt atacgggacg ccgaggttta caggcgtcat tgagaaggtt gaggtactgg
    16021 cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa acactacctt
    16081 ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt tgacgcaatg
    16141 gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata tcgtgacctg
    16201 cggagaagac tttggatcca cttggcgccc gcaaaatgat acagcgaatt tccggccggt
    16261 tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag gcattaagtt
    16321 ttcgtccata gttaaactca atgaccgcca ctttgttgat agtggcaacg ttcacatact
    16381 gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc actgagctgc
    16441 cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt ctggtggtaa
    16501 tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc ggtctgctta
    16561 cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact ggcagttagc
    16621 gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt ttccagtgcg
    16681 ctacaagtca gaccccgtgg ctgccattcg tcgtatttac gtcgcagaat atgcagggca
    16741 ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc gtctttgttg
    16801 ataacgagca gatcagccac ttccatcagc ccttttttaa tgccctgcag atcatcgccg
    16861 ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc cgtttccgac
    16921 tgcgcgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc gcataacagc
    16981 attaattccc gtgctcgctg actggcaccg cccagatgac cggaggaggg taccgggcga
    17041 ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc gagaatgcta
    17101 ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa tccctcacga
    17161 atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg tgtgccggta
    17221 acgcccagtc gcagggcgtt accacagaac ggcataatag cgtcaagcag ctgtgtactt
    17281 agcgcctgat gacgtgggtg acggctttcc accagcgtca tggcttgggc gagcgtggca
    17341 cgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt aatcatgatg
    17401 ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac ctggaccata
    17461 aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa tgacgccacc
    17521 cgcgaccacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca gttccgggat
    17581 cagcgtttta tgaccggcag ccagtgagga tgcgcccact acgtgaacgt cgttttctac
    17641 ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta cgtcgaaacc
    17701 gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat cctggcccat
    17761 cttagcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct ccgtttgcgc
    17821 aacaatggca tcgaactcgg aggccgattt ctcagactga tgatagcttt gcgcaatcac
    17881 gccggtaaca cactggcttg gcaccagata acggtcgaaa gcgacttcca gcgcatcgga
    17941 aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca gcaggttttc
    18001 gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg catcatcacg
    18061 ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca cgttgtcgat
    18121 ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac caacgatgac
    18181 acgcttgccc tggtcgatca gcgactgttc gcgcgctgag gcctcttcga tcattcgttt
    18241 tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct gttggataat
    18301 agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg atccggccag
    18361 tggatcgacg gtgtggcaga gttctgattc ttcctggatg atgatctggg tgttgcgggc
    18421 aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt tggtatgcag
    18481 tgactgagta ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga taacgttgtt
    18541 atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac gcagcgccag
    18601 tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat aacgtgccgc
    18661 acgcaacatg gcgacgttca taaacagatc catgccgata ccgaagaata acgacaggcg
    18721 aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt actcaatccc
    18781 atcggcgatc gtaaatgcta cctgctgcac gcagttggca cccgcttccc ccatgtggta
    18841 accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg cgatgatgtc
    18901 ggcgataatg cgcattgacg gttttggtgg gtaaatatag gtgttgcggc agaggtactc
    18961 tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac cttgctcttc
    19021 tgcggcgacg atataaaacg ccagtactgg tagcactgcg ccattcatgg tcatcgaaac
    19081 cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca cggtgtcgat
    19141 agcgacgccc gctttgccga cgtcacccgc cacgcgtggg ttatcggagt cgtagccacg
    19201 gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca ggttacggcg
    19261 ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga tggtccacgg
    19321 ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac caggaagggt
    19381 acctgtcacc tccagattat cgagatcggc ttcggtatac agcggcttga tggcgatccc
    19441 ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca attccttgtt
    19501 ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac gcaatcaaaa
    19561 tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc aaaaaaaatg
    19621 cattcagacg tcgttttttg ccgcctgaga tttgtctatt ttgtgatgtg attcaaggaa
    19681 tacaaaaaga agtgaggaca acgcctgata tgtgctatca ggcattattt gatggattaa
    19741 tcctgacgaa ggactttgtg accataatcg agtaacgcat cagtgacttt acgcatcatg
    19801 cggctttcag gagcaaagcg gtgccagtag agcatccgtc gttgaaatag cccaggcgtt
    19861 aagtcaatca gttcaccgct ggccagctct ttctcgattt gcaggtgcgg gatcatacag
    19921 caggtggtgc cctggcgagc aagttgtacg aacgcttctg aagaattaac gatatggcag
    19981 ggtacgctgc caggaggaag atcgaagttt tgctgcaaaa aggcctggtg catatcgtca
    20041 agatggtcaa acgcgaccac tggcgctttc aataatgccg aacgcgttac gccgttagga
    20101 aaatattttt cggcaaacgg ttttgagctg acgaacagat agtcgagcgc accgagttta
    20161 tcgacaagac aactcggcag cgcctgatgt tgaatactca ccgcgccgac cacttcgccg
    20221 cggcgcagac gttcctgagt acgggtttca tcttctacct gcaagttgag gcggataggc
    20281 gaatcagcca acacaggagc cagtgccgga agcaaccacg tcgccagact gtcggcgttg
    20341 accgccagtg aaagcagcag cggagtcgaa ccggtttgtt catcgcccag ccactcttct
    20401 tccagcaact ccacctggcg cagcagtgcc agcagttttt gcccttgttc cgtcgggcgc
    20461 ggcggtacgg tacgcaccaa cagcggctgc ccgaacatat tttccagttg cttaatgcgc
    20521 tgtgagacgg ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca
    20581 cgtatcaccg catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc
    20641 tgctcctggt aaatgctttt tcctgcacta tgacacaatt ttgtgtcagg ttgcatatac
    20701 cgaagccgtc ggcgggtaac atttgtatca atggttaaac gcgcagctta aaagatcagg
    20761 gatttgcgct attgcatttc gcttagcgac ttcgcgccct ggttatgttg aatggcgtgg
    20821 cgttattgcc tctgttcgca ccgtggagcg gcatacccag accgttgtat gctttatttg
    20881 cggggcgaaa ggggatgccc gccattgcgc gatattttat ggatgagtta accacccagc
    20941 ttgccaacgg ggtctgaatc gctttttttg tatataatgc gtgtgaaatt tcataccaca
    21001 ggcgaaacga tcatgacgca ggatgaattg aaaaaagcag taggatgggc ggcacttcag
    21061 tatgttcagc ccggtaccat tgttggtgta ggtacaggtt ccaccgccgc acactttatt
    21121 gacgcgctcg gcacgatgaa aggccagatt gaaggggccg tttccagttc agatgcttcc
    21181 actgaaaaac tgaaaagcct cggcattcac gtttttgatc tcaacgaagt cgacagcctt
    21241 ggcatctacg ttgatggcgc agatgaaatc aacggccaca tgcaaatgat caaaggcggc
    21301 ggcgcggcgc tgacccgtga aaaaatcatt gcttcggttg cggaaaaatt tatctgtatt
    21361 gccgacgcct ccaagcaggt tgatattctg ggtaaattcc cgctgccagt agaagttatc
    21421 ccgatggcac gtagtgcagt ggcgcgccag ctggtgaaac tgggcggtcg tccggaatac
    21481 cgtcagggcg tggtgaccga taatggcaac gtgatcctcg acgtccacgg catggaaatc
    21541 cttgacccga tagcgatgga aaacgccata aatgcgattc ctggcgtggt gactgttggc
    21601 ttgtttgcta accgtggcgc ggacgttgcg ctgattggca cacctgacgg tgtcaaaacc
    21661 attgtgaaat gatctgacgg gggaacctcc cccgttaaaa aaattctctt cattaaattt
    21721 ggtgacatgt gtcacgcttt taccaggcaa ttgtcgattg ctctaaataa atcctctaaa
    21781 ccagcatatt catccaagaa ttacctttgc gtgatatttc ctcaacatcg cgacgcaaac
    21841 gttcatattg ccgcaatatt attttttgat atgttgaaag gcggatgcaa atccgcacac
    21901 aacatttcaa aagacaggat tgggtaaatg gcaaaggtat cgctggagaa agacaagatt
    21961 aagtttctgc tggtagaagg cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt
    22021 tacaccaaca tcgaatttca caaaggcgcg ctggatgatg aacaattaaa agaatccatc
    22081 cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac
    22141 gccgcagaaa aactggtcgc tattggctgt ttctgtatcg gaacaaacca ggttgatctg
    22201 gatgcggcgg caaagcgcgg gatcccggta tttaacgcac cgttctcaaa tacgcgctct
    22261 gttgcggagc tggtgattgg cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat
    22321 gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc
    22381 aaaaagctgg gtatcatcgg ctacggtcat attggtacgc aattgggcat tctggctgaa
    22441 tcgctgggaa tgtatgttta cttttatgat attgaaaaca aactgccgct gggcaacgcc
    22501 actcaggtac agcatctttc tgacctgctg aatatgagcg atgtggtgag tctgcatgta
    22561 ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag agatttcgct aatgaagccc
    22621 ggctcgctgc tgattaatgc ctcgcgcggt actgtggtgg atattccggc gctgtgtgat
    22681 gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg
    22741 accaatagcg atccatttac ctctccactg tgtgaattcg acaacgtcct tctgacgcca
    22801 cacattggcg gttcgactca ggaagcgcag gagaatatcg gcttggaagt tgcgggtaaa
    22861 ttgatcaagt attctgacaa tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg
    22921 ctgccactgc atggtggtcg tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta
    22981 actgcgctga acaaaatctt cgccgagcag ggcgtcaaca tcgccgcgca atatctacaa
    23041 acttccgccc agatgggtta tgtagttatt gatattgaag ctgacgaaga cgttgccgaa
    23101 aaagcgctgc aggcaatgaa agctattccg ggtaccattc gcgcccgtct gctgtactaa
    23161 ttccccttct ctgaaaatta acgggcaggt cactgacttg cccgtttttt tatcccttct
    23221 ccacaccgtt gccgcgttgc gcattctact ttgcgagtct gctccagaaa ataaatcaaa
    23281 gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg atggcgggac agcatggggc
    23341 tgtaacggta aagccctcac cgaagcgagg gcttaaagga gaagggttat gatgcgactt
    23401 gtcatcatac tgattgtact gttactgata agtttcggag cttattaaca gtcagtctca
    23461 agggaggagt catcctccct tacccttact cactaaatta ggtcaaagaa tcaacgatgt
    23521 caatcagggc gatgcggggt gcatcgccct gaaccgaact ttttgatagc cacttcatcg
    23581 cccacgttta ctgccgtaat agatcctttt tcccaaaaat tatctctgtt ttgcatgggg
    23641 tttttcgagc acgctcgaag aaaagattta aaggtagatc aatgcgttgt ggttatttaa
    23701 agagagctta cgatagctgg gcagcatggg gctgtaacgg taaagccctc accgaagcga
    23761 gggcttgaag gagaagggtt atgatgcgac ttgccatcat actgattgta ctgttactca
    23821 taagtttccc agcttattaa cagtcagtca caggggagga gcaatcctcc cttaccctta
    23881 ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg tgtatcgccc
    23941 ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat cccactcttc
    24001 aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt gcgttttata
    24061 gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca ggcgctgacc
    24121 gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa ggggtagcac
    24181 gtcacgcaca tccagttttg gctcatggat cttcaacctg ttcatcacca gttcgctttg
    24241 cggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa ctggaagata
    24301 tacgcgctta ccggcgcgcc agagttgttc tattagcggc tgggtgtcga gttcaccatc
    24361 aaaagagagg aatacagcga ccgtatgcgc catcaccacc gggggataag tcatcatccg
    24421 ggtagcggct tgttgaccca tttccagctg ttgttccggc gttaacgcac gacgacgttg
    24481 ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagctctg gtagttgcgt
    24541 catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct gtaacccttg
    24601 aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga cgaaccgagc
    24661 atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct caggggactg
    24721 gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc acagtaaacc
    24781 gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac
    24841 gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc cgcgtagtca
    24901 cgagtctttg ctttttcttg cgctaactca tagctgatat tcaatgcggc aatgaagacc
    24961 aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg ttggttcaga
    25021 tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa
    25081 cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg ctgattgact
    25141 gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag ctaccctgat
    25201 gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat atcatgaata
    25261 ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg gttacaacga
    25321 aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgccgaga tgcatggttt
    25381 aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctgccgc tacttcacga
    25441 cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc gtaaaatgca
    25501 ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt atctgcctga
    25561 tggcgatgat gtcagcgttt tcgatcgggc tgatgcactg gcaggttggg tcaatcactt
    25621 cctgcttggt cttggcgtta cgcaaccgaa gctggacaaa gtgaccggcg aaaccggtga
    25681 agccatcgac gatctgcgta acatcgcgca gctgggctac gacgaagacg aagatcagga
    25741 agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg cgctgttatg
    25801 ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca
    25861 ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat atcccggcaa gagtttcagc
    25921 gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg atttttgctg
    25981 caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac agtgacttct
    26041 ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa agcgatgaca
    26101 ctcataacca cagcgttctg tttaaccgcg ttcgcgacct gacggcggag atctggtttg
    26161 gccgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc gcactggcat
    26221 tcagcgaaat caatcagcaa ctttatcaac tacttaacgg cctggatgtg gtttaccatg
    26281 cccagggcga atatgcatat gctgatgaaa tcgtgaacag tgcgctggaa aaactgcgta
    26341 aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt cctgttgttc
    26401 atgaaatgcg cctgttcaaa tcgccagaag agattgccgt actccgccgc gcgggagaaa
    26461 tcaccgccat ggcacataca cgggcgatgg aaaaatgccg tccgggaatg ttcgagtacc
    26521 atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgctat ccttcctata
    26581 acaccattgt aggcagcggt gaaaacggct gcattctgca ctacaccgaa aacgagagtg
    26641 aactgcgcga cggtgacctg gtgttgattg acgcgggttg tgaatacaaa ggttacgcgg
    26701 gcgatattac ccgcaccttc ccggtcaacg gcaaattcac ccaggcccag cgtgaaatct
    26761 acgacattgt gctggagtct ctcgaaacca gcctgcgcct gtatcgtccg ggaacttcca
    26821 ttctggaagt cactggtgaa gtggtgcgca tcatggttag cggcctggta aaactcggca
    26881 tcctgaaagg taatgttgat gaactgatcg ctcagaacgc ccatcgtcct ttctttatgc
    26941 atggccttag ccactggtta ggactggatg tccatgacgt tggcgtttat ggtcaggatc
    27001 gctcgcgcat tctggaaccg gggatggtac tgaccgtaga gcctggtctg tatatcgcgc
    27061 cggatgcgga tgtgccagaa caatatcgcg gtatcggcat tcgcattgaa gacgacattg
    27121 tgattaccga aaccggcaac gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa
    27181 tcgaagcgtt gatggctgct gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat
    27241 ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc tgccggtaca
    27301 tttgattgaa gcgactgcgc cagagtcaca tgctcatccg ggctttgatg gacgagcgat
    27361 agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc
    27421 tgattgcgca accgccatca ctaccgtgca tgtcagcgat cgtggtcatg ctggattcgt
    27481 caccctcgcc gcagaagatt accaactggc ggcgctgggg caggttgtcg aattgcataa
    27541 tgtcgggcaa cggctgtttg cattgctgcg aaaagcacct ggcgtaatgc tgcattgccc
    27601 cgatcgcgta gctaacgttg cccgtactca gagtcatgtt gaagtgacgc tggagagcgg
    27661 cgagatgctg acgggccgcg tgctgatagc cgctgatggc acccattcag cgttagccac
    27721 cgcgtgcggc gttgattggc agcaggagcc ttacgaacaa ctggcagtga ttgccaacgt
    27781 tgctacttct gttgcgcacg aaggccgcgc ttttgaacgc ttcacgcaac atggcccgct
    27841 ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc cactggaacg
    27901 gcgcgaagag gtgctgtcgt ggagcgacga gaagttttgc cgtgaactcc agtctgcctt
    27961 tggctggcga ctggggcaaa ttacccacgc cggtaaacgc agtgcttatc cgctggcatt
    28021 aacccgcgcc gccagaccga ttacccatcg cactgtgctg gtgggcaatg cggcgcaaac
    28081 tctgcacccg attgccgggc aagggtttaa cctcggtatg cgagatgtca tgagccttgc
    28141 ggaaaccctg actcaggcgc atgagcgcgg agaagacatc ggtgattacg gcatattgtg
    28201 ccgctatcag cagcgtcgac agagcgatcg cgaagcaacc atcggcgtca cggacagcct
    28261 ggtacatctt tttgccaacc gttgggcacc gctggttgtc gggcgcaaca tcggactgat
    28321 gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc tcggttgggt
    28381 ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta gccattgttg
    28441 gcggcggcat ggtggggctg gcggttgcct gcggcttaca ggggagcggc ttacgcgttg
    28501 ccgtactgga gcagcgcgta ccggaacctc tggcggtaga tgcactacca caactgcgcg
    28561 tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc tggcaggaca
    28621 ttctcgctcg tagggccagt tgttatcacg gcatggaagt gtgggacaaa gacagttttg
    28681 gtcacatctc gtttgacgat caaagcatgg gctatagcca tcttgggcac atcgttgaaa
    28741 actcagtgat tcactacgcg ctgtggaaca aagcgcagca gtcgtcagat attactctgt
    28801 tggcccccac agaattacag caggtcgcct ggggagaaaa tgaaaccttc ctgacgctga
    28861 aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggtgct aattcctggt
    28921 tgcgcaacaa agcagatatt ccgctgactt tctgggatta tcagcatcac gcgctggtag
    28981 cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg gcaggttttc catggcgaag
    29041 gcattctggc ctttttaccg cttagcgatc cgcatctttg ctcgattgtc tggtcactgt
    29101 cgccagagga agcgcagcgg atgcagcagg caagtgaaga cgaatttaat cgcgcgttaa
    29161 atatcgcttt tgataatcgc cttggcttat gcaaggttga gagcgcgcgt caggtgttcc
    29221 cgctgacggg gcgttatgcg cgccagtttg ccgcacaccg tctggcgctg gtgggcgacg
    29281 ccgcgcatac cattcaccca ctggcggggc agggcgttaa tctcggattt atggacgccg
    29341 cagagctgat tgctgaactg aaacggttgc atcgtcaggg taaagacatc gggcagtaca
    29401 tttacctgcg ccgctatgaa cgtagccgca agcacagcgc ggcgctgatg ctggctggta
    29461 tgcagggttt tcgcgatctc ttttccggcg ctaatccggc gaaaaaactg ctgcgtgata
    29521 ttggtttgaa actggccgac acgcttcctg gcgttaagcc gcaacttatc cgccaggcaa
    29581 tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcctctgt tgtttatttg
    29641 atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc atttgaaata
    29701 aactaatttc acctccgttt tcgcattata ttttctaata ccatgatttt ttgatttagt
    29761 gttttttgac atttttttag ctcttaatat tgtcttattc aaattgactt tctcatcaca
    29821 tcatctttgt atagaaattg gtgtattttt tggtttttta ttctgtcgcg atttttgcat
    29881 tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag tctgtttcgg
    29941 tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct ccgatgctgg
    30001 gttttgtggt gataatttca ccatgaaaaa gttgtcagcc ccgcttattc aatgaggaca
    30061 agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct cgcatggtgg
    30121 atttccacgg ctggatgatg ccgctgcatt acggttcgca aatcgacgaa catcatgcgg
    30181 tacgtaccga tgccggaatg tttgatgtgt cgcatatgac catcgtcgat ctccgcggca
    30241 gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag ctcaccaaaa
    30301 gaggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata gatgatctca
    30361 tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc acccgcgaaa
    30421 aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt accgttcgtg
    30481 atgacctttc catgattgcc gtgcaagggc cgaatgctca ggcaaaagct gccacactgt
    30541 ttaatgatgc tcagcgtcag gcggtggaag ggatgaaacc gttctttggc gtgcaggtgg
    30601 gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa attgcgctgc
    30661 ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggcgtt aagccctgcg
    30721 gtctgggggc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat ggtcaggaga
    30781 tggacgaaac tatttctcct ttagccgcca acatgggctg gactatcgcc tgggaaccga
    30841 cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat ggtacagaaa
    30901 aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg ccggtacgct
    30961 ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcggtact ttctccccga
    31021 cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg tattggcgaa acggcgattg
    31081 tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa acctgttttt gtgcgtaacg
    31141 gcaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg taccagcaga
    31201 actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca cttacaccgt
    31261 tggtattacc gaacatgctc aggagctgtt aggcgatatg gtgtttgttg acctgccgga
    31321 agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcgg taaaagcggc
    31381 gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg cactgagcga
    31441 ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc tggatcttta aaatcaaagc
    31501 cagcgatgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag cattgttaga
    31561 agatgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg aaaatttatg
    31621 cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg tttcaggaac
    31681 catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt attgaacgcc
    31741 atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggt gcacaatcgt
    31801 taaacgcgct gaccggccag attgtgccga aagatattca gcttgcgaca ccaccgcagg
    31861 ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt gccagtcgca
    31921 ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta ccgccggtta
    31981 tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg tatcagcctg
    32041 aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg ctggatttga
    32101 ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc gccgaagcaa
    32161 tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc gtggcttcca
    32221 atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc tgaaaccttt ggttttgaag
    32281 tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc gtgctgttac
    32341 agcaggtagg cactaccggt gaaattcacg actacactgc gcttattagc gaactgaaat
    32401 cacgcaaaat tgtggtcagc gttgccgccg atattatggc gctggtgctg ttaactgcgc
    32461 cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc gtgccgatgg
    32521 gctacggtgg cccacacgcg gcattctttg cggcgaaaga tgaatacaaa cgctcaatgc
    32581 cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg cgcatggcga
    32641 tgcagactcg cgagcaacat attcgtcgtg agaaagcgaa ctccaacatt tgtacttccc
    32701 aggtactgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg gttggcctga
    32761 aacgtatcgc taaccgcatt caccgtctga ccgatattct ggcggcgggc ctgcaacaaa
    32821 aaggtctgaa actgcgccat gcgcactatt tcgacacctt gtgtgtggaa gtggccgaca
    32881 aagcgggcgt actgacgcgt gccgaagcgg ctgaaatcaa cctgcgtagc gatatcctga
    32941 acgccgtggg gatcaccctt gatgaaacca ccacgcgtga aaacgtaatg cagcttttca
    33001 gtgtgctgct gggcgataac cacgggctgg agatcgacac gctggacaaa gacgtggctc
    33061 acgacagccg ctctatccag cctgcgatgc tgcgcgacga cgaaatcctc acccatccgg
    33121 tgtttaatcg ctatcacagc gaaaccgaaa tgatgcgcta tatgcactcg ctggagcgta
    33181 aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg aaactgaacg
    33241 ccgccgccga gatgatccca atcacttgga cggaatttgc cgaactgcac ccgttttgcc
    33301 cgccggaaca ggctgaaggt tatcagcaga tgattgcgca gctggctgac tggctggtga
    33361 aactgaccgg ttacgacgcc gtttgtatgc agccgaactc cggcgcacag ggcgaatacg
    33421 cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat cgcgatatct
    33481 gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg gcaggaatgc
    33541 aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat ctgcgcgcga
    33601 aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatcct tctacccacg
    33661 gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc ggcggtcagg
    33721 tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg ggctttattg
    33781 gcgcggacgt ttcgcacctc aacctgcata aaactttctg cattccgcac ggcggtggtg
    33841 gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc accgtttgta ccgggtcata
    33901 gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc agtttctgcg gcaccgttcg
    33961 gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc gcagaagggc
    34021 tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc cgcctgcagg
    34081 atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa tgtattctcg
    34141 atattcgccc gctgaaagaa gaaaccggca tcagcgagct ggatattgcc aagcgcctga
    34201 tcgactacgg tttccacgcg ccgacgatgt cgttcccggt ggcgggtacg ctgatggttg
    34261 aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg atgctggcta
    34321 tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc gctggaagat aacccgctgg
    34381 tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg ggcgcatccg tacagccgtg
    34441 aagttgcggt attcccggca ggcgtggcag acaaatactg gccgacggtg aaacgtctgg
    34501 atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc gaataccagt
    34561 aattcactga ttcgagtatt ttctaaaggc gcttcggcgc ctttttagtc agatgacaaa
    34621 gtacaaaagt gctcagacag tcccctcgcc cctttgggga gagggttagg gtgaggggaa
    34681 caggccggca ctggcgcgaa tatttaccct caccccgacc ctctccctga aagggcgagg
    34741 gggaaaagcg tgccaacatt gaagattgag ccagtttgtt agcaatctca aagatacgtc
    34801 aacgaattaa tttttctcgg aaaaacaaat ggctatagca cttgtgaccg gtggcagtcg
    34861 cggcatcggg cgggcaactg cattactgtt ggcgcaagaa gggtatacgg tggcggttaa
    34921 ttatcagcaa aacctccacg cggcgcagga agtgatgaac ttaataatgc aagccggtgg
    34981 caaagcattc gtgctccagg cggatatcag cgacgaaaac caggtcgttg cgatgtttac
    35041 agcaatcgat cagcacgatg aaccgctagc agcgctggtc aataacgccg ggatcttgtt
    35101 tacccagtgc accgttgaaa accttaccgc agagcgaatc aaccgagtac tttccaccaa
    35161 cgtgacggga tattttctct gctgccgcga ggcggtaaaa cgcatggcgc ttaaaaatgg
    35221 tggcagtggc ggcgctatcg tcaatgtctc ttcggtggcc tcacggttgg gttcgccagg
    35281 ggaatatgtt gattacgcgg catcgaaagg ggcgattgat tcgttaacca ccggactatc
    35341 gctggaagtc gccgcgcagg ggatccgcgt taactgcgtg cggccagggt ttatttatac
    35401 cgaaatgcac gccagcgcgg cgagcctgga cgcgtcgatc gcgttaagtc gaacatcccc
    35461 atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct actaagtgat
    35521 aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata aaacagggaa
    35581 gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta aagcttctcg
    35641 ccgttgctgg caatcacctc tttgtaccag ttaaagctct tcttacgtga acgcgacata
    35701 tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt gctgtactgc
    35761 ccggtagtga atgacacgca gtcgatgcag ccccacggtg tgtagcccat cagatccaca
    35821 ccatcgtaag tcactgcttt tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg
    35881 tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa accgttttcg
    35941 acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata gcgcagacct
    36001 actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg tacgctgcct
    36061 tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt ggtcatgtaa
    36121 tagctgaaac caagataatc gcaggtgcct tcgcgcagta catcgagatc gccgtcttcc
    36181 attttgatat taaatccgcg acgttcccac tcgttcaata cataggacgg gtaatagccg
    36241 catagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca
    36301 tcgtccgggt tacatgagta gggatagagc ggcaccatcg ccagcataca gccgactttc
    36361 atctccgggt taatgcgacg cgcggctttc actgccaggg cgctggcgac aaactggtga
    36421 tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtatacac cacgccggag
    36481 cagcagtaac caaacagcgg cgcacgccag ttacgctggt tgtttatttc gttgaaggtc
    36541 atccagtatt tgactttatg cttgtagcgt tcgaatacca cttccgcgaa acggacaaag
    36601 aaatcaacga atttacggtt ggtccagcta ccgtattgct gcaccagatg cagcggcatt
    36661 tcaaagtggg agagggttat caccggttcg atgttgtatt tcagcagttc atcgaacaga
    36721 gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt catcaccttt tggaaaaata
    36781 cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa tagcttgatg
    36841 tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta ttttcctagc
    36901 aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa
    36961 atgctcggtc ctttgccgcc tttgttccag ccgccttcga cctgatgagc ggcaacggcg
    37021 ccaccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg ctccagatca
    37081 gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt tcaacgtttc
    37141 caggatgaca ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac
    37201 ttcaaaacgg gtgatgtgct tcgtgtcgga cgttttgaag atgacggtta tttttgcacg
    37261 attgaagtca ccgcaacctc aaccgtaacg ctggatacac tgacggaaaa acatgcagag
    37321 caggaaaata tgaccctgcc tgaactgaaa aaggtcattg ctgacatcta tcccggtcag
    37381 acacaatttt atatgattga atttaaatgt ctttaaattg aactcacgcc tgttagttga
    37441 aatttcgcat ttaatttcat gatttttagg agattagtct caaatcagaa tttattttag
    37501 ctaacaggtg ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta
    37561 cgtttctctg gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc
    37621 agaggaaatt gccaacagcg tcagtcacgg cattgggctg gtgtttggta tcgttgggct
    37681 ggtgttgcta ctggttcagg cggtggatct caatgccagc gccacagcga taaccagtta
    37741 cagcctctat ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat
    37801 tcctcatcaa cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt
    37861 gattgccgga acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg
    37921 gttgatgatt gttatctgga gcctagcatt gctggggatt ctgttcaaac tgaccatcgc
    37981 gcaccgattc aaaattttat ctctggtgac ttatctggcg atgggctggc tgtcgctggt
    38041 ggtaatttat gaaatggcag ttaagctcgc ggcgggcagc gttaccttac tggcggtagg
    38101 cggcgtggtt tattcgctcg gggtgatttt ctacgtctgc aaacgcattc catacaacca
    38161 tgccatctgg cacggcttcg tgctcggcgg tagtgtgtgc cactttctgg cgatctattt
    38221 gtatattggg caggcgtaat gggtatgcct ttgtaggcct gataagacgc gtcagcgtcg
    38281 catcaggcat cggagcgcaa ccgccggatg cggcgtgaac gccttatccg atctacgggc
    38341 ctcgtccctg ttgtgataaa ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc
    38401 atcaggcgat aaatgcctga tgcgatacag agatttactc ttcgagcgaa tacggcagcg
    38461 gctcgatatg cagtgtattc gcatcgtcgc gtacgcggaa gatgctgtcc ggttccatat
    38521 cgttattcat gacgacctgt accacgacct gaccatcttc cagttttacc gcagccagca
    38581 ccgtaccggt acgacgccag ttctcgccca ttttcagctc taagtcttca ccagcttccg
    38641 gcagtcggct gacactacct gccagcaacc agagcgcgcg tttgttggca ccacggaatt
    38701 ttgctcgcgc caccatctct tgtccggtat aacagccttt cttaaagctg ataccgccca
    38761 gcgcctggag attggtcgcc tgtgggataa actgcccgct gttggcggca tcaatcaccg
    38821 ggaaaccagc ttcaatgttt aatgccagcc actgttggct attgttcagt tccgcttcac
    38881 cgcgcagttt atcggtcagc atattagcag tagcttcatc ggttacgatc aggaaacgtt
    38941 ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc tttgactacc tgtttttctt
    39001 tcgaaggcag ttcgctaaag agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac
    39061 caagcagcac acgctcgtcg tctggcgcga tggtcacttt agagaatacc gcatattttt
    39121 tcagttcagt cagctgcggt tcacgcacgc tgcgccgttc aatccatgca aagccatcgc
    39181 cgtcgcggaa cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga
    39241 gcaggtgctg atcttctgcc atctggctga catctgctgt cacctgaccc tgcatatatt
    39301 tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca
    39361 gcggtaaacg ggcagaagcc gtaggctgac gtggaggaaa aggtgtaaaa gccataatag
    39421 agtcctgagt tgcttaacgg cgtattaatg actaatggta aaagagctat gaggcaatgc
    39481 aagtgctttc actacgcgat ctgtgccttc tgcgaggcga ctgccaaaaa aatgaagccc
    39541 ttgaattgtt gtgaattaat ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag
    39601 aagtcgaaaa acacgttaca atagccgggt actggatttt cgtgagacag gaagaagatg
    39661 gacattaaca acaaagcccg aattcattgg gcatgccgcc gtggtatgcg cgaactcgat
    39721 atttcaatca tgccgttttt cgaacatgag tacgacagct taagcgatga cgaaaaacgc
    39781 atctttattc gtctgctgga atgtgacgat ccggacctgt ttaactggct gatgaatcac
    39841 ggtaaaccgg ccgatgcaga actggaaata atggtccgac tcatccagac acggaaccgg
    39901 gaacgtggtc ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt
    39961 gctgattcat gggctggttg ccgctgttat tttactcatg ccctggccgc tcagttacac
    40021 cccgttatgg atggtgttac tttcgctggt ggtatttgat tgcgttcgca gccagcggtg
    40081 tattaatgct cgccaggggg aaattcgctt gttgatggac gggcgtttgc gttggcaagg
    40141 gcaggagtgg agcatcgtca aagcaccgtg gatgattaag agcggcatga tgctgcgttt
    40201 acgttctgat ggcggtaaac ggcaacattt atggctggca gccgacagca tggacgaagc
    40261 cgaatggcgg gatttacggc ggattttgtt gcaacaagag acgcaaagat aaagatgacg
    40321 gcaaagcata tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc atttcgttga
    40381 ggatttgctc gcaccagctc tgaatacgct cgtcgctaag gtcatactgg ttagtttcat
    40441 ccagcgccag acccacgaac agttgcccgt cagcaatcac cggtttcggg ctggtaaatt
    40501 catatccttc cgttggccag tagccgacga acttcacgcc tttggtcgag agtttgtcat
    40561 gcagcatacc gagcgcatcg aggaaccact cgccgtatcc cagttgatcg ccaagcccat
    40621 acagcgcaat aattttacct tcaaggttca ggtcgtcgag ctgatcccag acggcttccc
    40681 agtcttcctg gatttcacca aaatcccagg tcgggatacc cagaatgagc acatcgtact
    40741 gctccattaa tttcggggag tcgtccttga ggttatgtaa ggtcaccagt tctgggccga
    40801 taatatcgcg gattttttct gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa
    40861 gacccatatt cataacgtaa gcttctcgat tctgctgtgg cttttgcagc tagtgtatca
    40921 gaatcgcttt agtcacggca caataaggca taatggatca aatgagtgac cataaggggc
    40981 gcaagtgaaa caggagctgg cacgcatcga gcagtttctt gatgctctgt ggctggaaaa
    41041 aaatctggct gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg
    41101 gttgcatcac cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc aggcattact
    41161 ggcagaacgg ctggagggcg ggtataaagc caccagctca gcgcgtttgc tgagtgcagt
    41221 gcgccgattg ttccagtatc tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca
    41281 tctcgcttca ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga
    41341 acgtttatta caggcgccat taattgatca gccactggag ctacgcgata aagccatgct
    41401 tgaagtgttg tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga caatgagtga
    41461 tatcagcctg cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt
    41521 gccgttaggg gaagaggcgg tttactggct ggaaacctat ctggaacatg ggcgtccgtg
    41581 gctgctgaat ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac
    41641 gcgacagacc ttctggcacc gcattaaaca ttatgccgtg ctggcgggta tcgacagcga
    41701 aaagctgtca ccgcatgtgt tgcgtcacgc ttttgccact catttattaa atcatggtgc
    41761 ggatttacgc gtggtgcaga tgctactggg ccacagcgat ctttccacca cgcaaattta
    41821 tacgcatgtc gctaccgagc gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg
    41881 atgtctgaaa agaacgggaa gatttatgaa gaaaggtttt atgttgttta ctttgttagc
    41941 ggcgttttca ggctttgctc aggctgatga cgcggcaatt caacaaacgt tagccaaaat
    42001 gggcatcaaa agcagcgata ttcagcccgc gcccgtagct ggcatgaaga cagttctgac
    42061 taacagcggt gtgttgtaca tcaccgatga tggtaaacat atcattcagg ggccaatgta
    42121 tgacgttagt ggcacggctc cggtcaatgt caccaataag atgctgttaa agcagttgaa
    42181 tgcgcttgaa aaagagatga tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt
    42241 gtttactgat attacctgtg gttactgcca caaactgcat gagcaaatgg cagactataa
    42301 cgcgctgggg atcaccgtgc gttatcttgc tttcccgcgc caggggctgg acagcgatgc
    42361 agagaaagaa atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt
    42421 gatggcaggt aaaagcgtcg caccagccag ctgcgacgtg gatattgccg accattacgc
    42481 acttggcgtc cagcttggcg ttagcggtac tccggcagtt gtgctgagca atggcacact
    42541 tgttccgggt taccagccgc cgaaagacat gaaagaattc ctcgacgaac accaaaaaat
    42601 gaccagcggt aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat
    42661 gaaacggcag acttgcccgc tgaattgcct cccttgctgc gccgtttata cgccagccgg
    42721 ggagtacgca gtgcgcaaga actggaacgc agtgttaaag gtatgttgcc ctggcagcaa
    42781 ctaagcggtg tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga aggaacgcgg
    42841 attattgtgg tcggtgattt cgacgccgac ggcgcgacca gcacggctct aagcgtgctg
    42901 gcgatgcgct cgcttggttg cagcaatatc gactacctgg taccaaaccg tttcgaagac
    42961 ggttacggct taagcccgga agtggtcgat caggcccatg cccgtggcgc gcagttaatt
    43021 gtcacggtgg ataacggtat ttcctcccat gcgggagttg agcacgctcg ctcgttgggc
    43081 atcccggtta ttgttaccga tcaccatttg ccgggcgaca cattacccgc agcggaagcg
    43141 atcattaacc ctaacttgcg cgactgtaat ttcccgtcga aatcactggc aggcgtgggt
    43201 gtggcgtttt atctgatgct ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat
    43261 gagcgcggca tcgcaattcc taacctggca gaactgctgg atctggtagc actggggaca
    43321 gtagcggacg tcgtgccgct ggacgctaat aatcgcattc tgacctggca ggggatgagt
    43381 cgcatccgtg ccggaaagtg ccgtccaggg attaaagcgc tgctggaagt ggcaaatcgc
    43441 gatgcacaaa aactagccgc cagcgattta ggttttgcgc tgggaccgcg tctcaatgct
    43501 gccgggcgac tggatgatat gtccgttggt gtggcgctgt tgctgtgcga caacatcggc
    43561 gaagcgcgcg tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa
    43621 caaggaatgc aggttgaagc cctgaccctg tgcgagaaac tggagcgcag ccgtgacacg
    43681 ctacccggcg ggctggcaat gtatcacccc gaatggcatc agggcgttgt cggtattctg
    43741 gcttcgcgta tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc agcaggtgac
    43801 ggtacgctga aaggttccgg tcgctccatt caggggctgc atatgcgtga tgcgctggag
    43861 cgattagaca cactctaccc cggcatgatg ctcaagtttg gcggtcatgc gatggcggcg
    43921 ggtttgtcgc tggaagagga taaattcgaa ctctttcaac aacggtttgg cgaactggtt
    43981 actgagtggc tggccccttc gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc
    44041 ccggccgaaa tgaccatgga agtggcgcag ctgctgcgcg atgctggccc gtgggggcag
    44101 atgttcccgg agccgctgtt tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc
    44161 gaacgtcatt tgaaggtgat ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt
    44221 gcttttaatg tcgataccgc tctctggccg gataacggcg tgcgcgaagt gcaactggct
    44281 tataagctcg atatcaacga gtttcgcggc aaccgcagcc tgcaaattat catcgacaat
    44341 atctggccaa tttagcgtca tcttctctat aaaaaagagc gtggattggg tacaatcccg
    44401 ctcttatcac cgcattttga ctagctcaat aaaagaaatc agaccatgtt tgaaattaat
    44461 ccggtaaata atcgcattca ggacctcacg gaacgctccg acgttcttag ggggtatctt
    44521 tgactacgac gccaagaaag agcgtctgga agaagtaaac gccgagctgg aacagccgga
    44581 tgtctggaac gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc
    44641 cgttgtcgac accctcgacc aaatgaaaca ggggctggaa gatgtttctg gtctgctgga
    44701 actggctgta gaagctgacg acgaagaaac ctttaacgaa gccgttgctg aactcgacgc
    44761 cctggaagaa aaactggcgc agcttgagtt ccgccgtatg ttctctggcg aatatgacag
    44821 cgccgactgt tacctcgata ttcaggcggg gtctggcggt acggaagcac aggactgggc
    44881 gagcatgctt gagcgtatgt atctgcgctg ggcagaatcg cgtggtttca aaactgaaat
    44941 catcgaagag tcggaaggtg aagtggcggg tattaaatcc gtgacgatca aaatctccgg
    45001 cgattacgct tacggctggc tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag
    45061 cccgtttgac tccggcggtc gtcgccacac gtcgttcagc tctgcgtttg tttatccgga
    45121 agttgatgat gatattgata tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg
    45181 cgcgtccggc gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca
    45241 catcccgacc gggatcgtga cccagtgcca gaacgaccgt tcccagcaca agaacaaaga
    45301 tcaggccatg aagcagatga aagcgaagct ttatgaactg gagatgcaga agaaaaatgc
    45361 cgagaaacag gcgatggaag ataacaaatc tgacatcggc tggggcagcc agattcgttc
    45421 ttatgtcctt gatgactccc gcattaaaga tctgcgcacc ggggtagaaa cccgcaacac
    45481 gcaggccgtg ctggacggca gcctggatca atttatcgaa gcaagtttga aagcagggtt
    45541 atgaggaacc aacatgtctg aacaacacgc acagggcgct gacgcggtag tcgatcttaa
    45601 caatgaactg aaaacgcgtc gtgagaagct ggcgaacctg cgtgagcagg gaattgcctt
    45661 cccgaacgat ttccgtcgcg atcatacctc tgaccaattg cacgcagaat tcgacggtaa
    45721 agagaacgaa gaactggaag cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac
    45781 ccgccgtatt atgggtaaag cgtctttcgt taccctgcag gatgtgggtg gccgcattca
    45841 gctgtacgtt gcccgtgacg atctgccgga aggcgtttac aacgagcagt tcaaaaaatg
    45901 ggacctcggc gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact
    45961 gtctatccac tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa
    46021 attccacggc ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg atctcatctc
    46081 taacgatgaa tcccgtaaca cctttaaagt acgctcgcag atcctctctg gtattcgcca
    46141 gttcatggtg aaccgcggct ttatggaagt tgaaacgccg atgatgcagg tgatcccagg
    46201 cggtgccgct gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg acatgtacct
    46261 gcgtatcgcg ccggaactgt acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt
    46321 cgaaatcaac cgtaacttcc gtaacgaagg tatttccgta cgtcataacc cagagttcac
    46381 catgatggaa ctctacatgg cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc
    46441 gctgttccgt actctggcac aggatattct cggtaagacg gaagtgacct acggcgacgt
    46501 gacgctggac ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata
    46561 tcgcccggaa accgacatgg cggatctgga taacttcgac tctgcgaaag cgattgctga
    46621 atctatcggt atccacgttg agaagagctg gggtctgggc cgtatcgtta ccgagatctt
    46681 cgaagaagtg gcagaagcac atctgattca gccgaccttc attactgaat atccggcaga
    46741 agtttctccg ctggcgcgtc gtaacgacat taacccggaa atcacagacc gctttgagtt
    46801 cttcatcggt ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg cggaagatca
    46861 ggcgcaacgc ttcctggatc aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt
    46921 ctacgatgaa gattacgtta ccgcactgga acatggctta ccgccgacag caggtctggg
    46981 gattggcatc gaccgtatgg taatgctgtt caccaacagc cataccatcc gcgacgttat
    47041 tctgttcccg gcgatgcgtc cggtgaaata agcattacgt tatgctcaca accccggcaa
    47101 atgtcggggt tttttattta agctgggtaa atgcagataa tcgtattctg gcttcgcgat
    47161 ttgtcgcctg catcaccatc cacggactga acgcccacgg cgtggcatca ataccgcgta
    47221 atacatcagc taaatcacac cattgataat ccatcacttc atcatcgttg atctgtaacg
    47281 cactggtggt gcgtgcggca aataccggac acacttcatt ttccacaatg cctctcggat
    47341 cggtggcgcg gtagcgaaag tcaggataga tagattcagg aggcgtaatt tccacgccaa
    47401 gctcataacg gcaacggcgg atcaccgcgt cttcgctgct ttctcccagt tgtgggtgcc
    47461 cacaaaccga gttagtccac acgccaggcc acgctttttt gctcagtgcg cggcgggtaa
    47521 ctaataattg ccccttggca ttaaacagcc aactggagaa cgcgagatgt aagagggtgt
    47581 ctgccgtgtg tgcggcatac ttttccagcg tacccgtggg aactccctgt gcattcaata
    47641 aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc atcattatag
    47701 ataattgaag agtgaaatga ttaaccaaga gagcgtttat cgacaaccgc ccgttttgag
    47761 cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga agcgactttc
    47821 aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacaacag
    47881 actgaatata gcgcgcatta aaaaattcat attattctcc atgctcattt ttcttcagct
    47941 tgcgcatcag aagccacata cgtactgtcc tgacttctaa atctttatca gagacggatt
    48001 ccttcaccag gtcagcatga tgctgatagc cattaaagaa gacgttcagc acaacggcgc
    48061 taagggttgc gagcataatg ccgctatgta gcagcggttg cagtacggcc ggtaacttag
    48121 aaaagaaatc gtgagagagc gtcggagtca tgccaacgcc gagactgatc gccacaatat
    48181 agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca
    48241 gtaccatgcc gaacatcact agaccagcgc cgcccagcac aaattgcgga atggaggcta
    48301 ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc gaagaaatac
    48361 acacccagcg gctatgaacg cgcatcacgc taaccaggcc aacgttttga gaaaatgacg
    48421 tgtgggggaa gctgttaaac gtgccgccta tcattgtccc tacgccatcg acacgcagcc
    48481 cgcaaataat atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca
    48541 tccccatcga ctcgataaac acgatgatt
//
LOCUS       DAJTNW010000021        48304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNW010000021 DAJTNW010000000
VERSION     DAJTNW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            177..470
                     /locus_tag="OO179_001256"
     CDS             177..470
                     /locus_tag="OO179_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5531388.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO179_001257"
     CDS             complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO179_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5531389.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1912..2364)
                     /locus_tag="OO179_001258"
     CDS             complement(1912..2364)
                     /locus_tag="OO179_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5531390.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO179_001259"
                     /gene_synonym="yicO"
     CDS             complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO179_001259"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5531391.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3926..5692
                     /gene="adeD"
                     /locus_tag="OO179_001260"
     CDS             3926..5692
                     /gene="adeD"
                     /locus_tag="OO179_001260"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5531392.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO179_001261"
     CDS             complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO179_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5531393.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO179_001262"
     CDS             complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO179_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5531394.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO179_001263"
     CDS             complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO179_001263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5531395.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO179_001264"
     CDS             complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO179_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5531396.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO179_001265"
     CDS             complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO179_001265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5531397.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO179_001266"
     CDS             complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO179_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5531398.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO179_001267"
     CDS             complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO179_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5531399.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13514..13603
                     /gene="tisB"
                     /locus_tag="OO179_001268"
     CDS             13514..13603
                     /gene="tisB"
                     /locus_tag="OO179_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5531400.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13883..15067
                     /gene="emrD"
                     /locus_tag="OO179_001269"
     CDS             13883..15067
                     /gene="emrD"
                     /locus_tag="OO179_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5531401.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15075..15572)
                     /locus_tag="OO179_001270"
     CDS             complement(15075..15572)
                     /locus_tag="OO179_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5531402.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15569..15931)
                     /locus_tag="OO179_001271"
     CDS             complement(15569..15931)
                     /locus_tag="OO179_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5531403.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15921..16268)
                     /locus_tag="OO179_001272"
     CDS             complement(15921..16268)
                     /locus_tag="OO179_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5531404.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16377..16790
                     /locus_tag="OO179_001273"
     CDS             16377..16790
                     /locus_tag="OO179_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531405.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16837..18279)
                     /locus_tag="OO179_001274"
                     /pseudo
     CDS             complement(16837..18279)
                     /locus_tag="OO179_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18314..20029)
                     /locus_tag="OO179_001275"
     CDS             complement(18314..20029)
                     /locus_tag="OO179_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5531406.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20196..21089
                     /locus_tag="OO179_001276"
     CDS             20196..21089
                     /locus_tag="OO179_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5531407.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21086..21900)
                     /locus_tag="OO179_001277"
                     /pseudo
     CDS             complement(21086..21900)
                     /locus_tag="OO179_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21900..23517)
                     /locus_tag="OO179_001278"
                     /pseudo
     CDS             complement(21900..23517)
                     /locus_tag="OO179_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23812..24486
                     /locus_tag="OO179_001279"
     CDS             23812..24486
                     /locus_tag="OO179_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5531408.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24525..26186)
                     /locus_tag="OO179_001280"
     CDS             complement(24525..26186)
                     /locus_tag="OO179_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5531409.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26382..26810)
                     /gene="ibpB"
                     /locus_tag="OO179_001281"
     CDS             complement(26382..26810)
                     /gene="ibpB"
                     /locus_tag="OO179_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5531410.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26922..27335)
                     /gene="ibpA"
                     /locus_tag="OO179_001282"
     CDS             complement(26922..27335)
                     /gene="ibpA"
                     /locus_tag="OO179_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5531411.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27329..27401)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27640..27972
                     /locus_tag="OO179_001283"
     CDS             27640..27972
                     /locus_tag="OO179_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5531412.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27974..29194)
                     /locus_tag="OO179_001284"
     CDS             complement(27974..29194)
                     /locus_tag="OO179_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5531413.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29295..30395
                     /gene="cbrA"
                     /locus_tag="OO179_001285"
     CDS             29295..30395
                     /gene="cbrA"
                     /locus_tag="OO179_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5531414.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30356..31693)
                     /locus_tag="OO179_001286"
     CDS             complement(30356..31693)
                     /locus_tag="OO179_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5531415.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31768..32916)
                     /gene="dgoD"
                     /locus_tag="OO179_001287"
                     /pseudo
     CDS             complement(31768..32916)
                     /gene="dgoD"
                     /locus_tag="OO179_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32913..33530)
                     /gene="dgoA"
                     /locus_tag="OO179_001288"
     CDS             complement(32913..33530)
                     /gene="dgoA"
                     /locus_tag="OO179_001288"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5531416.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33514..34392)
                     /gene="dgoK"
                     /locus_tag="OO179_001289"
     CDS             complement(33514..34392)
                     /gene="dgoK"
                     /locus_tag="OO179_001289"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5531417.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34389..35078)
                     /gene="dgoR"
                     /locus_tag="OO179_001290"
     CDS             complement(34389..35078)
                     /gene="dgoR"
                     /locus_tag="OO179_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5531418.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35356..36012
                     /locus_tag="OO179_001291"
     CDS             35356..36012
                     /locus_tag="OO179_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531419.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36058..36870)
                     /gene="yidA"
                     /locus_tag="OO179_001292"
     CDS             complement(36058..36870)
                     /gene="yidA"
                     /locus_tag="OO179_001292"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5531420.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36984..37382)
                     /locus_tag="OO179_001293"
     CDS             complement(36984..37382)
                     /locus_tag="OO179_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5531421.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37622..40036)
                     /gene="gyrB"
                     /locus_tag="OO179_001294"
     CDS             complement(37622..40036)
                     /gene="gyrB"
                     /locus_tag="OO179_001294"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5531422.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40065..41138)
                     /gene="recF"
                     /locus_tag="OO179_001295"
     CDS             complement(40065..41138)
                     /gene="recF"
                     /locus_tag="OO179_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5531423.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41138..42238)
                     /gene="dnaN"
                     /locus_tag="OO179_001296"
     CDS             complement(41138..42238)
                     /gene="dnaN"
                     /locus_tag="OO179_001296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5531424.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42243..43646)
                     /gene="dnaA"
                     /locus_tag="OO179_001297"
     CDS             complement(42243..43646)
                     /gene="dnaA"
                     /locus_tag="OO179_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5531425.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43943..44023
                     /locus_tag="OO179_001298"
     CDS             43943..44023
                     /locus_tag="OO179_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531426.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44253..44393
                     /gene="rpmH"
                     /locus_tag="OO179_001299"
     CDS             44253..44393
                     /gene="rpmH"
                     /locus_tag="OO179_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5531427.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44410..44769
                     /gene="rnpA"
                     /locus_tag="OO179_001300"
     CDS             44410..44769
                     /gene="rnpA"
                     /locus_tag="OO179_001300"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5531428.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44733..44990
                     /gene="yidD"
                     /locus_tag="OO179_001301"
     CDS             44733..44990
                     /gene="yidD"
                     /locus_tag="OO179_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5531429.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44993..46639
                     /gene="yidC"
                     /locus_tag="OO179_001302"
     CDS             44993..46639
                     /gene="yidC"
                     /locus_tag="OO179_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5531430.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46745..48109
                     /gene="mnmE"
                     /locus_tag="OO179_001303"
     CDS             46745..48109
                     /gene="mnmE"
                     /locus_tag="OO179_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5531431.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11991 a        13058 c        12202 g        11053 t
ORIGIN      
        1 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
       61 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
      121 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
      181 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      241 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      301 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      361 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      421 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      481 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      541 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      601 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      661 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      721 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      781 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      841 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      901 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      961 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
     1021 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     1081 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1141 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1201 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1261 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1321 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1381 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1441 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1501 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1561 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1621 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1681 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1741 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1801 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1861 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1921 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1981 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     2041 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     2101 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2161 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2221 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2281 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2341 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2401 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2461 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2521 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2581 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2641 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2701 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2761 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2821 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2881 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2941 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     3001 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     3061 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3121 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3181 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3241 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3301 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3361 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3421 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3481 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3541 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3601 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3661 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3721 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3781 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3841 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3901 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3961 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     4021 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     4081 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4141 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4201 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4261 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4321 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4381 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4441 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4501 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4561 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4621 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4681 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4741 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4801 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4861 gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc tgaatcacct
     4921 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4981 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     5041 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     5101 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5161 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5221 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5281 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5341 ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     5401 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5461 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5521 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5581 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5641 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5701 gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5761 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5821 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5881 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5941 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     6001 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     6061 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6121 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6181 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6241 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6301 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6361 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6421 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6481 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6541 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6601 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6661 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6721 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6781 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6841 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6901 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6961 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     7021 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     7081 gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg ggttactcct
     7141 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7201 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7261 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7321 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7381 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7441 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7501 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7561 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7621 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7681 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7741 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7801 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7861 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7921 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7981 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     8041 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     8101 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8161 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8221 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8281 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8341 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8401 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8461 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8521 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8581 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8641 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8701 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8761 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8821 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8881 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8941 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     9001 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     9061 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9121 ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9181 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9241 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9301 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9361 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9421 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9481 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9541 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9601 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9661 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9721 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9781 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac taccgatcat
     9841 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9901 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9961 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
    10021 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    10081 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10141 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10201 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10261 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10321 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10381 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10441 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10501 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10561 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10621 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10681 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10741 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10801 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10861 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10921 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10981 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    11041 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    11101 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11161 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11221 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11281 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11341 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11401 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11461 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11521 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11581 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11641 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11701 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11761 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11821 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11881 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11941 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    12001 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    12061 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12121 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca gcggcttttt
    12181 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12241 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12301 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12361 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12421 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12481 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12541 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12601 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12661 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12721 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12781 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12841 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12901 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12961 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    13021 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    13081 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13141 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13201 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13261 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
    13321 ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13381 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13441 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13501 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13561 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13621 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13681 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13741 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13801 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13861 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13921 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13981 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    14041 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    14101 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14161 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14221 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14281 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14341 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14401 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14461 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14521 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14581 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14641 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14701 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14761 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14821 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14881 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14941 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    15001 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    15061 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15121 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15181 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15241 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15301 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15361 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15421 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15481 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15541 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15601 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15661 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15721 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15781 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15841 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15901 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccacc
    15961 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    16021 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    16081 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16141 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16201 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16261 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16321 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16381 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16441 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16501 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16561 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16621 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16681 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16741 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16801 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16861 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16921 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16981 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    17041 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    17101 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17161 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17221 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17281 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17341 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17401 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17461 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17521 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17581 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17641 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17701 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17761 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17821 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17881 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17941 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    18001 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    18061 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18121 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18181 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18241 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18301 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18361 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18421 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18481 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18541 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18601 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18661 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18721 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18781 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttgg
    18841 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18901 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18961 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata
    19021 acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc agcgtcgggt
    19081 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19141 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19201 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19261 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19321 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19381 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19441 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19501 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19561 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19621 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19681 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19741 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19801 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19861 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19921 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19981 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    20041 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    20101 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20161 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20221 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20281 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20341 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20401 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20461 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20521 agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt cagcaaagcg
    20581 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20641 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20701 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20761 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20821 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20881 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20941 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    21001 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    21061 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21121 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21181 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21241 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21301 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21361 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21421 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21481 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21541 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21601 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21661 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21721 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21781 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21841 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21901 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21961 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    22021 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    22081 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22141 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22201 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22261 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22321 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22381 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22441 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22501 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    22561 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    22621 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    22681 agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag actgaactct
    22741 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    22801 aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    22861 taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    22921 atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag
    22981 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    23041 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    23101 ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc
    23161 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    23221 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    23281 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    23341 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    23401 ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc cggcgaaggg
    23461 aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac tgagcatttc
    23521 cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg gcagacctgg
    23581 agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt cgtgatcgct
    23641 ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag acatctgtga
    23701 gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa tcgattaagc
    23761 agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga gatgatctac
    23821 aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac gctaaacagc
    23881 cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat gactatccgt
    23941 aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg cagcggcact
    24001 tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact ggtggaggtg
    24061 ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat catgcctgcg
    24121 cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta cttctcccgt
    24181 cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat gccggtaaaa
    24241 ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata tattgaacaa
    24301 gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct cgccgataga
    24361 ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat cacctcgttg
    24421 tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa tgccagcgaa
    24481 tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc gatactccag
    24541 aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa cggatagaca
    24601 gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc aaacgccagc
    24661 gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta attcattttg
    24721 gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat cagggcacca
    24781 taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc cccaccagat
    24841 ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc gaggttggca
    24901 cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag gatcaacgcc
    24961 ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc tggcacaaag
    25021 acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg cagcatctga
    25081 acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc aattgcggac
    25141 ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc actggcgacc
    25201 agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc tttaaagtgc
    25261 aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg ctcgacgcgc
    25321 aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac cagttgcgca
    25381 ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg gataatggta
    25441 tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat aattttgtcg
    25501 ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt ctcaacgcga
    25561 atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct
    25621 tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa caaaatcccg
    25681 caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac catttccatt
    25741 ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt attggttacc
    25801 gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt atgcaggatg
    25861 gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag gcgtaatccg
    25921 gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt ataaacgaac
    25981 aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt catccccgcc
    26041 tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat acctaatcct
    26101 atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa agccagaata
    26161 ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat tttaattgct
    26221 acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta tcacgggaaa
    26281 gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga agaaagacaa
    26341 aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt taacgcggga
    26401 cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg aattaaatca
    26461 atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc agccagcgta
    26521 aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt ctcttctttt
    26581 ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat ctctaaatct
    26641 tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc gcttttctca
    26701 atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg
    26761 tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat agtcatttct
    26821 ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct ccctgacggc
    26881 gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat ttcgatacgg
    26941 cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa accatttacc
    27001 aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc aaagttgcgt
    27061 tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc
    27121 accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa accagccaca
    27181 gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg agggtagccg
    27241 ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa tccaatagca
    27301 gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg aaatcagcga
    27361 gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc aggcgcgtcc
    27421 tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta tcagcggact
    27481 tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa cgttaaagtg
    27541 cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga tcgctataac
    27601 tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa tgtgttgtta
    27661 gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt gatgtcccat
    27721 accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat gattggcgac
    27781 gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt taccgctgta
    27841 atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt gcgctcgcgc
    27901 gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc ggccagaatg
    27961 cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct tccatccacg
    28021 ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga ttggcgtggt
    28081 tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt cgattatcca
    28141 gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga atatccgttt
    28201 tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac agttgcacaa
    28261 tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc
    28321 agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag gttaaacgtc
    28381 gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt aacggcgcat
    28441 cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat agctccggca
    28501 ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc
    28561 atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt
    28621 tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc ttttgtacgt
    28681 tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc ggatcgagtt
    28741 gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg ctaaagacat
    28801 gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat
    28861 cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg aagtcgtaat
    28921 gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct gacttcggat
    28981 gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc
    29041 ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca
    29101 cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggta ttggtgagca
    29161 ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacggttc
    29221 ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga
    29281 atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcagggt
    29341 cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat aaaaagcacc
    29401 agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg gacgcgcagc
    29461 gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat ccacagattt
    29521 tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag cgaagctata
    29581 tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc gccagcgttg
    29641 aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa tggcatgtca
    29701 tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc ggtgccgatg
    29761 gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg
    29821 ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc atctttgata
    29881 atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt atctttggcg
    29941 gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag aaaatgagcg
    30001 cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg ttgtttccct
    30061 cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt gaagcggcgg
    30121 gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc gcagagattc
    30181 tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg cgcgccacac
    30241 acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg
    30301 ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg aaagattatc
    30361 caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc aacgcgacgg
    30421 cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac cccaccacca
    30481 gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg gttaaaccaa
    30541 tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca aaaccgttgc
    30601 cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg tagttagcgc
    30661 ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc gcaaagccca
    30721 gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg acgccgacaa
    30781 acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg attccttttt
    30841 cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa gccaccgcaa
    30901 attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag tctttggctg
    30961 ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga ccgccgccat
    31021 cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc ggctgataaa
    31081 ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg aacacccagt
    31141 gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc agaccgacaa
    31201 actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg aaccagctgg
    31261 tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt atcgcacgca
    31321 gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcacc actgaccaac
    31381 caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac caaccgccgg
    31441 ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc atttccgctt
    31501 tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaagtta gcgcggtcaa
    31561 cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc cgacgccccg
    31621 gctttgctgc attaacggga atatccatag cgagctttct ccagattttg ggcatagcga
    31681 agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc tgttttagtg
    31741 cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat cttcatgacg
    31801 ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt tagcttcgtc
    31861 gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa ccatgctgaa
    31921 gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat gaatgcccat
    31981 actttgttac tgaagtaccg cgttatagga aacaaagtcg atatgcaggc aagccgccag
    32041 tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt ctgccattcc
    32101 agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt gcagaatcga
    32161 aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc gctcacccgc
    32221 cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt gttccgccag
    32281 caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca gcactttcgc
    32341 cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat tgccaaaagc
    32401 ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt tatcaattag
    32461 ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc gcagggtttt
    32521 aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg ctttaatttt
    32581 gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt tgcctttgat
    32641 atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac cgccacgata
    32701 aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat cctgaccaat
    32761 caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc cttcaatcac
    32821 gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca tccagcgggg
    32881 aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg atacgcctta
    32941 acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg ataaagatcg
    33001 ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt ttctggcgtc
    33061 acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa cgctttgatg
    33121 tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc cgcttcgagc
    33181 gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc gtagcccacc
    33241 gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca gcccatcctg
    33301 gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa cgccttgtcg
    33361 ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt cagcgggatt
    33421 tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc ctcgtcgggc
    33481 gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat gagcgatgct
    33541 ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc aacccatcgc
    33601 ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg tgatggcgtg
    33661 ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca aaccagagag
    33721 aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc gaacttcaaa
    33781 aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg cgaaggcatc
    33841 ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta acagtaaatg
    33901 atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat cggcctgcac
    33961 ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag ctcgcgcgcc
    34021 gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag agacgcataa
    34081 tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa tagacgaaaa
    34141 acgggcagga acagataaat acggagcaac tttccagccg acattgctgc cgaccattcc
    34201 tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg ctaacaccac
    34261 agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc tctccaggca
    34321 gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa ttgcgatgta
    34381 gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat ggtaagcgcc
    34441 gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc cttatgttcc
    34501 tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa aaccgcccgc
    34561 tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac cgactgcagc
    34621 accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg attgttggca
    34681 atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc gcgctctgct
    34741 gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc actgataagc
    34801 cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag gtaattccac
    34861 tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag ccgcttcgcc
    34921 atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc ttcacagagc
    34981 tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat ctgtttaccc
    35041 agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg tgctctgtat
    35101 gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt accagcgtga
    35161 tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgtta tgctacaatt
    35221 tagatcaaaa aaagaacaat acataaaaaa atgacatgcg tcgggcagaa atctgaaaag
    35281 ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct tagctactcg
    35341 tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc cggtttattc
    35401 ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca taccgcaaag
    35461 ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc gcaggcaaaa
    35521 gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta ccttatcact
    35581 gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg gcacaatatt
    35641 caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt taccggcaca
    35701 atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac gcattatggt
    35761 tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg cacaatccat
    35821 gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc atttaaagtg
    35881 cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc agcattactg
    35941 ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt tgtatatgca
    36001 gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc ttacagatta
    36061 attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg attttgtgac
    36121 aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac cgacgcctgc
    36181 atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt cttccggttt
    36241 aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc gtttatcgag
    36301 gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca cttcctgcgg
    36361 aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca ctttcaggaa
    36421 ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg tggcaacgaa
    36481 ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg tggtgcggtc
    36541 cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac gatagtcgtc
    36601 atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta ccagcgcgcc
    36661 gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca ggtagttgtg
    36721 cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg cgcgagctgc
    36781 ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc
    36841 catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag ttcctgaccc
    36901 atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg ggatgcaacg
    36961 cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag ttccatgcct
    37021 gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga gagcgcatca
    37081 ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc cacgccaagt
    37141 ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg ctcgccgcta
    37201 actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc taagccgcca
    37261 ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca acttaaaatc
    37321 gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc aaaaagcccc
    37381 atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct gcgggatgat
    37441 aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc ttatagcttc
    37501 ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgcaaaa tcaatatatt
    37561 gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat ggttaacgcc
    37621 attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca cgacgcggtt
    37681 ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca tccttaacgg
    37741 taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc tgttccgggt
    37801 tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac tctttcacca
    37861 gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca cgttcgataa
    37921 acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata cgacgatatt
    37981 cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg tgggtacgca
    38041 cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca aacttccact
    38101 ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa
    38161 cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata agctctttca
    38221 gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc gcgttgtact
    38281 cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg ttggtgtgca
    38341 gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg tcgtctttaa
    38401 tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg atgtagacgt
    38461 gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa
    38521 tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa cgcagtttgt
    38581 ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc gtcgccactt
    38641 cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt ttacccttca
    38701 gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg cccgcggagt
    38761 ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt
    38821 tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac
    38881 gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcatcag
    38941 ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca accgccgatt
    39001 tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg tccggcactt
    39061 ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg ctgactttgg
    39121 cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc atcgccgcac
    39181 ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta aagcagtaga
    39241 tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata ccgtcttttt
    39301 cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc agatattcaa
    39361 cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc
    39421 gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt ttcgccagaa
    39481 tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag aaacgcacca
    39541 tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt
    39601 cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt ttttgcgaca
    39661 gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta taggagttat
    39721 cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc gatacgccct
    39781 cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag acagagttat
    39841 cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg tcgatagcgt
    39901 tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat
    39961 acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata ctggaggagt
    40021 cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc tgttatttta
    40081 cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac gtgttcagca
    40141 ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc aagcaacccg
    40201 cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca ccgccgcccg
    40261 ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc
    40321 tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct
    40381 ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt acgttccagc
    40441 acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt cagagaaaac
    40501 tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc gctatactcc
    40561 gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca cgggcgtagc
    40621 tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga
    40681 ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc gaggaatgct
    40741 cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat caactgcatc
    40801 ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat acggactttg
    40861 ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc gccctgtaat
    40921 cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg
    40981 cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt tttgccactg
    41041 ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc ggtttcaata
    41101 ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt ggcatgacaa
    41161 cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc gaatcggtca
    41221 gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca tagctgacgt
    41281 tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct tccgcttctt
    41341 cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac aggcgcacac
    41401 cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg agcagatcgc
    41461 agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa tccgggaagc
    41521 gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga atgttattgc
    41581 tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc agttcaatca
    41641 cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt ggcattgaac
    41701 agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct tcggtttcaa
    41761 acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac tgggttgctt
    41821 caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc tgccagtcat
    41881 cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta cgcccggagc
    41941 gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca ggcagaccac
    42001 ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg tgtggctgaa
    42061 ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc gacaacgtac
    42121 cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga ccacctaacg
    42181 gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta aatttcatag
    42241 gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc gtggctctct
    42301 tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc acgcccacca
    42361 aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag cgccatcgcc
    42421 atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc cgcgactttg
    42481 atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag tttttcctgc
    42541 aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta
    42601 aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac gttagatcgt
    42661 agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt ttcgtcggcc
    42721 tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc cacagtcagt
    42781 ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt cggatagcga
    42841 tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt gtggaaaaac
    42901 tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag cagtgcatct
    42961 acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc tttaaccatg
    43021 tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt gcgcgccata
    43081 atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc gccataaagg
    43141 aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc cgcgcgcgcc
    43201 agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa
    43261 cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca
    43321 gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct cgtcactgcc
    43381 gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg tggggcatcc
    43441 gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc ccgtacccaa
    43501 tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc
    43561 aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa tcgggcaaga
    43621 cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt cgataatgac
    43681 taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg
    43741 cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc
    43801 ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc cctatttatc
    43861 cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt
    43921 acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc gatctaataa
    43981 agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca cccggcgcgc
    44041 catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc
    44101 gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat ccggcctctt
    44161 taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc aataaagcca
    44221 ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac
    44281 tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg
    44341 ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag taataaagct
    44401 aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac tcccagtcaa
    44461 ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac cattctcggc
    44521 cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa aaacgttcga
    44581 cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg tctgcgccaa
    44641 catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat
    44701 aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc
    44761 gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta ctcgggccgc
    44821 attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc aggtttggag
    44881 tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct ttacaccctg
    44941 gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa cgatggattc
    45001 gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg
    45061 ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa cgaccaccgc
    45121 agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac tgatctcggt
    45181 taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg agcaagctct
    45241 gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt tggaaacttc
    45301 accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc cggataaccc
    45361 ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg
    45421 tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa
    45481 aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc
    45541 tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca tcactctgcc
    45601 accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg gcgcggcgta
    45661 ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg ataacgaaaa
    45721 cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt tcgcgacggc
    45781 gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg gtaacggcat
    45841 cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga ctggcgcgat
    45901 gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg ttgctccgca
    45961 cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc tgttcaaact
    46021 gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca tcatcacctt
    46081 tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca tggcgaagat
    46141 gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg
    46201 tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc tgggcggctg
    46261 cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc tgatgggttc
    46321 cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg cacaggaccc
    46381 gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc
    46441 gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg tcatcttcac
    46501 cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca gcaacctggt
    46561 aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc tgcatagccg
    46621 cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg
    46681 ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa gcaacaggaa
    46741 catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac gtggcggcgt
    46801 tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa
    46861 actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg gcagcgtgct
    46921 cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg aagatgtgct
    46981 ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac gcattctgac
    47041 cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga
    47101 taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca gttcggaaca
    47161 ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg ttaatcatct
    47221 ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt tccctgatga
    47281 agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg tgatggccga
    47341 tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag ggatgaaagt
    47401 agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc tggcgggccg
    47461 tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc gtgagcatat
    47521 ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg aggccagtga
    47581 cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt
    47641 gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga tctggccgga
    47701 atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata aagccgatat
    47761 caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc gtctctcggc
    47821 aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca tgggctttga
    47881 caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc tggaacaggc
    47941 agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag gtgaactgct
    48001 ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg aatttacttc
    48061 agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac cgcgcttacg
    48121 aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc tgttatgcct
    48181 caaccctttt ttaaacatta aaattcttac gtaatttata atctttaaaa aaagcattta
    48241 atactgctcc ccgaacgatt gtgattcgat tcacatttaa acaatttcag aatagacaaa
    48301 aatt
//
LOCUS       DAJTNW010000022        47877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNW010000022 DAJTNW010000000
VERSION     DAJTNW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            517..1071
                     /gene="pinE"
                     /locus_tag="OO179_001304"
                     /pseudo
     CDS             517..1071
                     /gene="pinE"
                     /locus_tag="OO179_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1410..2258)
                     /locus_tag="OO179_001305"
     CDS             complement(1410..2258)
                     /locus_tag="OO179_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5531432.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2467..3102
                     /gene="yfhb"
                     /locus_tag="OO179_001306"
     CDS             2467..3102
                     /gene="yfhb"
                     /locus_tag="OO179_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5531433.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            3127..3663
                     /gene="tadA"
                     /locus_tag="OO179_001307"
     CDS             3127..3663
                     /gene="tadA"
                     /locus_tag="OO179_001307"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5531434.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(3660..5216)
                     /gene="mltF"
                     /locus_tag="OO179_001308"
     CDS             complement(3660..5216)
                     /gene="mltF"
                     /locus_tag="OO179_001308"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5531435.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            5475..9362
                     /gene="purL"
                     /locus_tag="OO179_001309"
                     /gene_synonym="purI"
     CDS             5475..9362
                     /gene="purL"
                     /locus_tag="OO179_001309"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5531436.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            9938..11365
                     /gene="qseE"
                     /locus_tag="OO179_001310"
     CDS             9938..11365
                     /gene="qseE"
                     /locus_tag="OO179_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5531437.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            11530..12243
                     /gene="qseG"
                     /locus_tag="OO179_001311"
     CDS             11530..12243
                     /gene="qseG"
                     /locus_tag="OO179_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5531438.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            12233..13567
                     /gene="glrR"
                     /locus_tag="OO179_001312"
     CDS             12233..13567
                     /gene="glrR"
                     /locus_tag="OO179_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5531439.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            13628..13966
                     /gene="glnB"
                     /locus_tag="OO179_001313"
     CDS             13628..13966
                     /gene="glnB"
                     /locus_tag="OO179_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5531440.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14011..15201)
                     /gene="hmpA"
                     /locus_tag="OO179_001314"
     CDS             complement(14011..15201)
                     /gene="hmpA"
                     /locus_tag="OO179_001314"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5531441.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            15528..16781
                     /gene="glyA"
                     /locus_tag="OO179_001315"
     CDS             15528..16781
                     /gene="glyA"
                     /locus_tag="OO179_001315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5531442.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(16979..18172)
                     /locus_tag="OO179_001316"
     CDS             complement(16979..18172)
                     /locus_tag="OO179_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5531443.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            18290..18463
                     /locus_tag="OO179_001317"
     CDS             18290..18463
                     /locus_tag="OO179_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531444.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            18470..21571
                     /locus_tag="OO179_001318"
     CDS             18470..21571
                     /locus_tag="OO179_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5531445.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            21668..22651
                     /locus_tag="OO179_001319"
     CDS             21668..22651
                     /locus_tag="OO179_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5531446.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            22674..24185
                     /locus_tag="OO179_001320"
     CDS             22674..24185
                     /locus_tag="OO179_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5531447.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            24210..25208
                     /locus_tag="OO179_001321"
     CDS             24210..25208
                     /locus_tag="OO179_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5531448.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            25241..26335
                     /locus_tag="OO179_001322"
     CDS             25241..26335
                     /locus_tag="OO179_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5531449.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            26347..27219
                     /locus_tag="OO179_001323"
     CDS             26347..27219
                     /locus_tag="OO179_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5531450.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(27267..27689)
                     /locus_tag="OO179_001324"
     CDS             complement(27267..27689)
                     /locus_tag="OO179_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5531451.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(27786..28988)
                     /locus_tag="OO179_001325"
     CDS             complement(27786..28988)
                     /locus_tag="OO179_001325"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5531452.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(28998..29810)
                     /gene="hcaB"
                     /locus_tag="OO179_001326"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(28998..29810)
                     /gene="hcaB"
                     /locus_tag="OO179_001326"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5531453.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(29807..30127)
                     /locus_tag="OO179_001327"
     CDS             complement(29807..30127)
                     /locus_tag="OO179_001327"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5531454.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(30127..30645)
                     /locus_tag="OO179_001328"
     CDS             complement(30127..30645)
                     /locus_tag="OO179_001328"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5531455.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(30642..32003)
                     /locus_tag="OO179_001329"
     CDS             complement(30642..32003)
                     /locus_tag="OO179_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5531456.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            32139..33029
                     /gene="hcaR"
                     /locus_tag="OO179_001330"
     CDS             32139..33029
                     /gene="hcaR"
                     /locus_tag="OO179_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5531457.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            33189..34328
                     /locus_tag="OO179_001331"
     CDS             33189..34328
                     /locus_tag="OO179_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5531458.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(34320..35600)
                     /gene="csiE"
                     /locus_tag="OO179_001332"
     CDS             complement(34320..35600)
                     /gene="csiE"
                     /locus_tag="OO179_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5531459.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35791..36645)
                     /locus_tag="OO179_001333"
     CDS             complement(35791..36645)
                     /locus_tag="OO179_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5531460.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(36790..37593)
                     /gene="suhB"
                     /locus_tag="OO179_001334"
     CDS             complement(36790..37593)
                     /gene="suhB"
                     /locus_tag="OO179_001334"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5531461.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            37712..38452
                     /gene="trmJ"
                     /locus_tag="OO179_001335"
     CDS             37712..38452
                     /gene="trmJ"
                     /locus_tag="OO179_001335"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5531462.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            38722..39210
                     /gene="iscR"
                     /locus_tag="OO179_001336"
     CDS             38722..39210
                     /gene="iscR"
                     /locus_tag="OO179_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5531463.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            39322..40536
                     /gene="iscS"
                     /locus_tag="OO179_001337"
     CDS             39322..40536
                     /gene="iscS"
                     /locus_tag="OO179_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5531464.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            40564..40950
                     /gene="iscU"
                     /locus_tag="OO179_001338"
     CDS             40564..40950
                     /gene="iscU"
                     /locus_tag="OO179_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5531465.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            40967..41290
                     /gene="iscA"
                     /locus_tag="OO179_001339"
     CDS             40967..41290
                     /gene="iscA"
                     /locus_tag="OO179_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5531466.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            41386..41901
                     /gene="hscB"
                     /locus_tag="OO179_001340"
     CDS             41386..41901
                     /gene="hscB"
                     /locus_tag="OO179_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5531467.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            41918..43768
                     /gene="hscA"
                     /locus_tag="OO179_001341"
     CDS             41918..43768
                     /gene="hscA"
                     /locus_tag="OO179_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5531468.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            43770..44105
                     /gene="fdx"
                     /locus_tag="OO179_001342"
     CDS             43770..44105
                     /gene="fdx"
                     /locus_tag="OO179_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5531469.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            44117..44317
                     /gene="iscX"
                     /locus_tag="OO179_001343"
     CDS             44117..44317
                     /gene="iscX"
                     /locus_tag="OO179_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5531470.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            44376..45659
                     /gene="pepB"
                     /locus_tag="OO179_001344"
     CDS             44376..45659
                     /gene="pepB"
                     /locus_tag="OO179_001344"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5531471.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            45801..46577
                     /gene="sseB"
                     /locus_tag="OO179_001345"
     CDS             45801..46577
                     /gene="sseB"
                     /locus_tag="OO179_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5531472.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(47398..>47877)
                     /gene="sseA"
                     /locus_tag="OO179_001346"
     CDS             complement(47398..>47877)
                     /gene="sseA"
                     /locus_tag="OO179_001346"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5531473.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT        11345 a        11988 c        13055 g        11489 t
ORIGIN      
        1 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
       61 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat ttgccataag ggaatgagtg
      121 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
      181 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
      241 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
      301 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
      361 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
      421 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
      481 ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta
      541 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
      601 ctgatttttg aagacaagat aagtggtaca aagtccgaaa gaccggggct gaaaaaactg
      661 ctcaagacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
      721 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
      781 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
      841 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
      901 gctgctcgcg cacagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
      961 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     1021 gatgttggcg aatcgacttt gtataagagg tttccagcca gggttatata aaaccgcaga
     1081 cacggcgtat acaagaacgt gctacggttg actgaaaagt taatgttgta tgtcacaggc
     1141 cttttgcgcc gtaaagcccc gtcctttagg gcgcggagga cgttaaagaa agtggtgttc
     1201 tttgtgttgc tgaacttatg caaaattttt ttgcaccata aatccaacaa cacattcaat
     1261 aacacattgc tgtatcacta tgaaaataaa tgtgttttta tttgatttca cacatatcac
     1321 aattgatgca ttttttagtg attaacagcg ccataaggaa actctcaaaa caacacaaac
     1381 cgggcgggca ttatacgcgc attctttact cagaccagtt ttttcaccag cgcttcacta
     1441 tgacgaatac gttctggtgc cagttccaga tcctgctgaa tcagcgcaat gaacagcaaa
     1501 tccgttaaca ttccctgagc gtgacaagca gagattgaag cactgtttgt cgcttgttct
     1561 tcggcaatgg tatacaggca atgagaagca cgctgttgca gggcattcgg agtaaagccg
     1621 gtaatcgcca gcacttttcc gcccactcgc agcatctcat ctgccgccag gtttaactcg
     1681 cgtcgtacac cggtgtagga aatggctaat aacagatcgt caggggacga cgcctgtact
     1741 gttgcgagta gcgcatgcat atcgcgcagt gcggcagcat tgaagccaat cttcatcagc
     1801 ttccaggcaa agttttgcgc caccagaccc gaagcgccaa taccggtcag aattatccgc
     1861 cgcgcagagc gcaacattgt tacgcattca tgcagtttct cttcactatt aacgtttagc
     1921 gttgcgtaca tagcggcagt attttcttta atcagttttt cgccgaccag ccgtaacgga
     1981 tcatcaccgc ggatttggtt atgaatgggc acggagggcg attccggctg gcttgccagc
     2041 gcttcactca acgccaactt aagcgccgga aaacctttat agccgagttt ttgcgcgaac
     2101 ttcacgacgc tggactgact gactccggct tcgttggcca gttgctgaga gcttaaatgg
     2161 cgcgccgtat caggttgtag cagcagataa tccgccagtt ttttatcact ctgggcaagc
     2221 ccttggtaac gctgacggat acgaagtaag ccgttcatat ctttcctcgc gatgaaatag
     2281 tggcgacaac aggcaatatg agacacacgc gagtgaattt tatattccat tgccgctaat
     2341 tgccatgaat taaaaattcc agttagccat gatgcggtcg attgttgccg gaaaagtgat
     2401 gatctatctg ttaggctatg tagtagcgaa aattaatcag gatgtttcag tccagaggag
     2461 tatggtttgg caactcacga gcgtcgtgtg gtgttttttg acttagatgg aacattgcat
     2521 cagcaggata tgttcggcag ttttctgcgc tatttactgc gtcgccaacc gctgaatgcg
     2581 ttacttgtcc tgccgttgtt accgattata gccattgcgt tattgataaa aggtcgtgcg
     2641 gcacgctggc cgatgagtct gcttctgtgg gggtgcactt ttggtcacag cgaagcacgt
     2701 ttacagacgt tgcaggccga tttcgtgcgc tggtttcgcg acaatgttac cgcctttccg
     2761 ctggttcagg agcgattaac cacctacctg ttaagttccg atgctgatat ctggttgatt
     2821 accggctctc cgcagccgct ggttgaagcg gtttatttcg atacgccctg gctgccgcgg
     2881 gttaatctta tcgccagcca aattcagcgt ggctatggtg gttgggtatt gacgatgcgt
     2941 tgtctgggac atgaaaaggt cgcacaactg gagcgcaaaa tcggcacgcc gctgcggctg
     3001 tacagtggct atagcgacag taatcaggac aatccgctgc tttatttctg tcagcatcgt
     3061 tggcgagtaa ccccgcgcgg tgaactccag caactggaat agagtaaagc atagcgtccg
     3121 tgtataatgc gccgcgcttt tataaccgga gttttctttt tgtctgaagt cgaatttagc
     3181 cacgaatatt ggatgcgtca cgcgctgacg ctggcgaaac gtgcctggga tgagcgggaa
     3241 gtgccggtcg gcgcggtatt agtgcataac aatcgggtaa tcggcgaagg ctggaaccgc
     3301 ccgattggtc gccatgatcc caccgcacat gcagaaatca tggccctgcg gcagggtggt
     3361 ctggtgatgc aaaattatcg tctgatcgac gccacgttgt atgtcacgct tgaaccatgt
     3421 gtaatgtgtg ccggagcgat gatccacagt cgcattggtc gcgtggtctt tggtgcgcgt
     3481 gacgcgaaaa ctggcgctgt gggatcttta atggatgtgc tgcatcatcc gggtatgaat
     3541 caccgagtgg aaattacgga aggaatactg gcggatgagt gcgcggcgtt gctcagtgac
     3601 ttctttcgca tgcgccgcca ggaaattaaa gcgcagaaaa aagcgcaatc ctcgacggat
     3661 taattttgtt tctcttcact ccctttcctg gaaaacagca gagagggaga atgggtcaaa
     3721 tagtttgatg acgactggga aagaaacgag agaaaaggaa atttctcttt gcccaactcc
     3781 gtaggcgata ccgccggata atcctgcgcc agttgcatcg ccgcttctgt agcctgcttc
     3841 tctttctctt gcagataacc caccaggcta atctgatact tacgaatatt ttcgacataa
     3901 gcgtaggctt catgcccacg agcgtagccg taagtcagct tgctgtaata gggtttctgg
     3961 ctaagtaaag gcagacgctg ttttacgtca gcccaactgt caggattccc tttggttttt
     4021 gccgtcaggg cgcgggcatc cagcatatgc gcatagccca tattgtacgc agcgagggca
     4081 aaccagatcc gttcgttctc cggcacactt tccggcactt tactcatcat atcctgcaaa
     4141 taacgcacgc caccgctgat gctctgttcg gcatcggtac gatccgtaat gccgaggctt
     4201 tgcgcggtat ttttggttaa catcatcatg ccgcgcacac ccgtcggtga agtggcctgt
     4261 gcatcccagt gcgattcctg ataagcaata gcggccagca aacgccagtc aatttcttcg
     4321 gcgtatttct caaacagggg ctttaactgc ggcagtaccg catcgacggc gcgtaaaaat
     4381 gtgcgcgtat cgacgtaatc aaaatcatcg ccatgcccca ggtatttctc ttcaatgcgt
     4441 gccagcgtac cgtcttcatt catttcgttg aagaagtcga gcagggcggc ggaaagggta
     4501 ttatcgccat ctaacgggct aaaccaagtc accggttgtt catcggtgat atcgagcgct
     4561 acggcgagct ccgggtgaac gcgctgaaac aggctgatgg cgacagaatc agcaatggtg
     4621 taatcgagtt ttccttcgat gacatcttcc attaattccg cagagccttt tttgtcgtct
     4681 accttccagc ttaattccgg gaattttgtt tctttcaggg tctggagatc gttaaccacc
     4741 acatgacccg gtgcaacggt gagttgctcc gccgtcaggt tgcccagcgt acgtgggcga
     4801 tactgaccca ctttataaac cagttgttgt gacacggaat aataggtagg gccaggctga
     4861 taatttttta cccgctcact gttatagacc agtcctgccg ccagcaggtc ggcgttacca
     4921 ttatcaaggt cgtcaaacag ctggctgata ttctgccgca cggtcacttt cagttttacg
     4981 ccgaggtaat cggcaaactg tttcgccagt tcgtaatcta ggccaaaagg tttcccgttg
     5041 atttcgttat aagtcagggg agtatgaatg gtgctcacac gcaactctcc ccgcgcttga
     5101 atggcggcga tacggttgtc ggctttacca aaccagggaa tggatggcca gagagcgacc
     5161 gcgagcagca gtgccagaat gccgatgaac agataattaa tctttaattt tttcaattag
     5221 ttaattctct gtgtcgtgcg cgtcccagct tgaaaaaacg taataatagt gaaaggttta
     5281 ctcataaatg agcggcattt tgcgtaaacc tgcgccagat ggcaacttat tacagccatt
     5341 ggcggcacgc gttgctaatt cacgatggtg attttatttc cacgcaaacg gtttcgtcag
     5401 cgcatcagat tctttataat gacgcccgtt tcccccccct tgggtacacc gaaagcttag
     5461 aagacgagag acttatgatg gaaattctgc gtggttcgcc tgcactgtcg gcattccgaa
     5521 tcaacaaact gctggcacgt tttcaggctg ccaggctccc ggttcacaat atttacgccg
     5581 agtatgtcca ttttgctgac ctcaatgcgt cgttaaacga tgatgagcac gcacaacttg
     5641 aacgcctgct gaaatatggt ccggcactcg ccagccacgc cccgcaaggc aaactcctgc
     5701 tggtgacccc gcgtcctggc accatctctc cctggtcttc gaaagcgacc gatattgccc
     5761 ataactgcgg gctacaacag gtaaaccgcc ttgagcgcgg cgttgcttac tatatagaag
     5821 ccggtacgct gaccaatgaa caatggcagc aggttaccgc tgaactgcac gaccgcatga
     5881 tggaaacggt cttttttgat ttagatgatg cagagcagtt gtttgcccac catcaaccga
     5941 ctccggtcac aagcgttgat ttgctggggc agggccgtca ggcgctgatc gacgctaacc
     6001 tgcgtcttgg cttggctctg gcggaagatg aaattgacta tctgcaggat gctttcacaa
     6061 agcttggtcg taacccgaac gacatcgagc tgtatatgtt cgctcaggcg aactctgagc
     6121 actgccgcca caaaattttt aacgccgact ggattatcga cggtgaacaa cagccgaaat
     6181 cgctgttcag gatgatcaaa aatactttcg aaaccacgcc agatcacgtt ctctctgctt
     6241 ataaagataa cgccgccgta atggaaggtt ctgaagtggg ccgctacttt gctgaccacg
     6301 aaacgggccg ctacaatttc catcaggaac cggcgcacat cctgatgaaa gtcgaaactc
     6361 acaaccaccc gacggcgatt tctccgtggc cgggcgcggc gaccggttcc ggcggtgaaa
     6421 tccgcgatga aggtgccacc gggcgcggcg cgaagccgaa agctggtttg gttggtttct
     6481 ccgtatccaa cctgcgaatt cctggcttcg aacagccgtg ggaagaagat ttcggtaagc
     6541 ctgagcgcat tgttactgcg ctggacatca tgaccgaagg cccgctgggc ggtgcggcgt
     6601 ttaacaacga atttggtcgt ccggcactga acggctactt ccgtacttat gaagaaaaag
     6661 tgaacagcca caatggcgaa gagctgcgcg gttatcacaa accgatcatg ctggcgggcg
     6721 ggatcggcaa cattcgcgcc gatcacgtac aaaaaggcga gatcaacgtc ggtgcgaagc
     6781 tggtcgttct cggcggcccg gcaatgaaca tcggtcttgg cggcggcgcg gcgtcttcca
     6841 tggcgtctgg tcagtctgat gccgacctcg actttgcttc cgtacagcgc gacaacccgg
     6901 agatggagcg tcgttgccag gaagtgatcg accgttgctg gcagcttggt gatgctaacc
     6961 caattctgtt tatccacgac gtcggtgcgg gcggtctttc taacgcgatg ccggagctgg
     7021 tgagcgacgg cggacgcggc ggtaaatttg aactgcgcga tattctaagc gacgaaccgg
     7081 gcatgagccc gctggaaatc tggtgtaacg aatcccagga acgctacgtg ctggcggttg
     7141 ctgccgatca attaccgctg tttgacgaac tgtgtaagcg tgagcgcgca ccctacgcgg
     7201 tgattggtga agcgaccgaa gaactgcatc tttctctgca cgatcgtcat tttgataatc
     7261 agccgatcga tctgccgctg gacgtcctgc ttggtaaaac gccgaagatg acccgcgatg
     7321 tacaaacgct gaaagcgaag ggcgatgcgc tggtccgtga agggatcacc attgcagacg
     7381 cggtgaaacg cgtactgcat ctgccgaccg tggcggagaa aactttcctg gtgaccatcg
     7441 gtgaccgcag cgtaaccggc atggtggcgc gcgatcagat ggtggggccg tggcaggtgc
     7501 cggtcgctaa ctgcgcggtc actaccgcca gcctcgacag ctactacggt gaagcgatgg
     7561 cgattggcga gcgtgcgccg gttgcgctgc tggatttcgc cgcctctgcc cgtctggcgg
     7621 tcggtgaagc gttaaccaat attgccgcaa cacagattgg cgatatcaaa cgcattaaac
     7681 tttccgccaa ctggatggcg gcggcaggcc accctggtga agatgcgggc ctgtatgaag
     7741 ccgttaaagc cgtgggtgaa gaactttgtc cggcgctggg cctgacgatc ccggtgggta
     7801 aagactctat gtcgatgaaa acccgctggc aggaaggtaa cgaagagcgc gaaatgacgt
     7861 cgccgctgtc gctggtgatt tctgcatttg cccgcgtgga agatgtacgt cacaccatca
     7921 cgccgcagct ttgtaccgaa gataacgcac tgctgctgat tgatttgggc aaaggcaata
     7981 acgcgctggg cgcaacggcg ctggcgcagg tttatcgtca gcttggcgac aaaccggcag
     8041 atgtacgcga tgttgtccaa ctgaaaggct tctatgacgc gattcaggcg ctggttgcac
     8101 agcgtaagct gctggcgtat cacgaccgct ctgatggagg cctgctggta acgctggcgg
     8161 aaatggcctt tgcaggtcat tgtggcattg acgcggatat cgcctctctg ggtgacgatc
     8221 gcctggcggc gttgtttaac gaagaactgg gtgcggtgat tcaggttcgt gccgctgacc
     8281 gtgaagcggt cgagtccgta ctggcacagc atgggcttgc ggattgcgtc cattatgttg
     8341 ggcaggcggt ttccggtgac cgttttgtga ttaccgccaa cgggcagact gtattcagcg
     8401 aaagccgcac tacgttgcgt gtctggtggg cagaaactac ctggcagatg cagcgcctgc
     8461 gtgacaaccc ggagtgtgcc gatcaggagc atcaggcgaa atctaacgac gccgatccgg
     8521 gcctgaatgt aaaactgtcg ttcgatatca acgaagatgt ggcagcaccg tatattgcca
     8581 ctggcgcacg tccgaaagtt gccgtactgc gtgagcaggg cgtgaactcg catgttgaaa
     8641 tggcggcagc tttccaacgt gggggctttg atgctatcga cgtgcatatg agcgacctgc
     8701 tgactggacg tacgggactg gaggattttc acgccctggt cgcgtgcgga ggtttctcct
     8761 acggtgatgt gctgggtgcc ggtgaaggtt gggcgaagtc tatcctgttc aatgaccgtg
     8821 tacgcgatga gtttgcaacc ttcttccatc gtccgcaaac gctgtccctg ggggtatgta
     8881 acggttgcca gatgatgtct aacctgcgtg aactgatccc ggggagtgag ttgtggccac
     8941 gttttgtgcg taacacctcc gatcgctttg aagcgcgttt cagcctggtt gaagtaaccc
     9001 aaagcccgtc tctgctgttg caggggatgg tgggctcgca aatgccgatt gctgtctctc
     9061 atggtgaagg gcgcgtggaa gtgcgtgatg cggcgcatct ggcggcactg gaaagcaaag
     9121 ggctggtggc actgcgctat gtcgataact tcggcaaagt cactgaaacc tacccggcta
     9181 acccgaacgg ttcaccgaac ggtattacgg cggttacgac tgaaagtggt cgagtcacca
     9241 ttatgatgcc gcacccggaa cgtgttttcc gtactgtcag caactcctgg catccggaaa
     9301 actggggcga ggatggccca tggatgcgca ttttccgcaa tgcgcgtaag cagttggggt
     9361 aagtcatcag ttcattggtt ttgcagcccg gatgcggcat ttacgccgca tccggcatta
     9421 agcaccaacc actatcataa gctcccaact ccgggagctt ttttgtgtct gtaaatcacg
     9481 acaatgggtg gtttaccgtg tcgctttctg gcgacactta aatgattgat tttattgtaa
     9541 tctaataagt ttatcttaag ttgtctcttt ttagcgacac agtggctgat ttacattcaa
     9601 atagcgaaga agccataaat ccctcaaagc aacgtataat cagtacgtta ccaaactatt
     9661 ttctttattg gcacagttac tgcataatag taagcagtgg ctcattcacc gacttatgtc
     9721 agccccttcg ggacgtgcta cataaaattc gaatgacgca caacaaggtg cctgccgtcc
     9781 aacttctgat atcagcgtag ctatatcaac catcgggcga aacgtcgagt taggcaccgc
     9841 cttattccat aacaaagccg ggtaattccc ggctttgttg tatctgaact tcccctcggt
     9901 tagcatcagg ctattcgcgt ctgacgagag taacaccttg aaacgctggc ccgtttttcc
     9961 ccgctcatta cgacaactgg taatgctggc atttttgcta attctactac ccctgttggt
    10021 gctggcatgg caagcctggc aaagcctgaa tgcgcttagc gatcaggcgg cgctggttaa
    10081 ccgcactacg cttattgatg cccggcgtag tgaagcgatg accaacgcgg cgctggagat
    10141 ggagcgtagc taccgtcagt attgcgtgct ggacgaccct acgcttgcga aggtttatca
    10201 aagccagcgc aagcgttaca gcgaaatgct cgatgcccac gcaggcgtac tgccagacga
    10261 taaactttac caggcattgc gtcaggattt gaacaatctg gcccaacttc agtgtaacaa
    10321 cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc tttgccagtg ccaataccga
    10381 aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg cagcaacttc agcgtgaaat
    10441 cgccgaacgt gggcaatatt ttggttggca atcgctggtg ctatttctgg tgagtctggt
    10501 gatggtgcta cttttcacgc ggatgattat cgggccggtg aaaaatatcg agcgcatgat
    10561 caaccgtctg ggggaagggc gttctctggg caatagcgtt tcgttctgtg gaccgagcga
    10621 gttacgctcg gttgggcaac gtattctttg gttaagtgag cgcctgtcat ggctggaatc
    10681 ccaacgccat caatttttaa gacatttatc tcatgaatta aaaacgccac tggcgagtat
    10741 gcgcgagggc actgaattac tggctgatca ggttgtcggg ccgcttacgc cagagcaaaa
    10801 agaggtggtg agcattcttg atagcagcag ctgcaatttg caaaaactga tcgaacaact
    10861 gcttgattac aaccgtaaac aggcggacag tgcggtggaa ctggagaatg ttgaattagc
    10921 accgctggtg gagacagtgg tttctgctca tagcctgccc gcacgggcta aaatgatgca
    10981 taccgacgtc gatctcaaag caacagcttg cctggcggag ccaatgctgc tgatgagcgt
    11041 actggataat ctttactcca atgcggtgca ctacggggct gaatccggta acatttgcct
    11101 tcgcagcagt ttacatggtg cgcgggttta tattgatgtc atcaatacag gcacgcccat
    11161 tccgcaagag gaacgcgcca tgatcttcga cccctttttt cagggaagcc accagcgaaa
    11221 aggggcggtg aagggcagcg gtctgggatt aagcattgcc agggattgta ttcgccgtat
    11281 gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac gtttgtttcc gcattgaatt
    11341 accgtcgtcg aaaaacacga aataatgcta acagcctatc ctatcaggct attttatttg
    11401 tcagttcggc cccgggtagt gctcgaaatc ttcacgtacg atatgtacgc cccggtttct
    11461 ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc cactgggata ggctttaagt
    11521 ctggtgaata tgcgacacat ttttcaacga ttattgcccc gacggctgtg gctggcaggg
    11581 ctgccatgtc tggcattgct gggttgcgtg cagaatcaca ataagccagc cattgatacg
    11641 ccagcagaag aaaaaattcc ggtttatcaa ctggctgact atctttctac tgaatgtagc
    11701 gacatctggg cgctgcaagg taaatcaacc gaaaccaatc cgctttactg gctgcgggcg
    11761 atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc agcaagctcg ccaatatgac
    11821 gacggcagct ggcaaaatac ctttaaacaa gggattttgc tcgccgacgc caaaattacg
    11881 ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt taagtaccga gatcccggca
    11941 caggttcgcc cactgtatca actctggcgc gacggtcagg cgctgcaact gcaactggcg
    12001 gaagagcgcc aacgttacag taaactccag cagtccagcg acagcgagct ggatacattg
    12061 cgccagcaac atcacgttct acagcagcaa ctggaactca ccacccgcaa gctggaaaac
    12121 ctgaccgata ttgaacgcca gctctcgacc cgcaaaccgg ctggaaattt ctcgccggat
    12181 acgccgcacg agagtgaaaa gcccgcgcca tccacccatg aggtcactcc tgatgagcca
    12241 taaacctgcg catttattat tggtcgatga cgatccggga ttgctgaaac tgcttggcct
    12301 gcgcctgacc agcgaaggct acagtgtggt cacggcggaa agtggcgctg aaggattacg
    12361 ggtactgaat cgcgaaaaag tagatttagt tatcagcgac ctgcggatgg atgaaatgga
    12421 cggtatgcag ctatttgctg aaatccagaa agtgcagccg ggaatgccag taattattct
    12481 tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca cagcagggcg tttttagttt
    12541 cctcaccaag cctgtcgata aagatgcgct atatcaggca attgacgatg cgctggagca
    12601 atccgcgcca gccaccgatg aacgctggcg cgaggcaatt gtcacccgca gcccgctgat
    12661 gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca gacgtcagcg ttttgattaa
    12721 cggtcagagc ggcaccggga aagagatttt cgcccaggct atccacaacg ccagcccgcg
    12781 caacagcaaa ccatttattg ctattaactg tggcgcattg cccgagcaat tgctggagtc
    12841 ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct gtcagcaatc gcgaaggttt
    12901 attccaggcg gcggaaggcg gaacgctatt tctcgatgaa attggcgata tgcctgcgcc
    12961 gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa gtgcgcccgc tgggcagtaa
    13021 ccgcgatatt gatatcaatg tgcggattat ttctgccact caccgtgatc tgtcaaaagc
    13081 gatggcgcgc ggggaattcc gcgaagatct ctattaccgc ctcaacgttg tcagcctgaa
    13141 aattccggca ctggcggagc gcacagaaga cattccgcta ctggcaaatc acctgttgcg
    13201 ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc tctaccgatg cgatgaaacg
    13261 cctgatgacc gcgagctggc cgggtaatgt gcgccagttg gtcaacgtga ttgaacagtg
    13321 cgtggcgctg acctcatctc cggtgattag tgatgcgctg gtggagcagg cgctggaggg
    13381 tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat cattttgaac tcaactattt
    13441 gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac gcggctagaa tggcggggcg
    13501 caaccggaca gaattttata aactgctttc ccgacacgag ctggatgcaa acgatttcaa
    13561 ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa agacaggcaa ccttttcaag
    13621 gaatagcatg aaaaagattg atgcgattat aaaacccttc aagctggacg atgtccgcga
    13681 agcactggcc gaagtcggta ttaccggcat gacggtgacc gaagtgaaag gctttggtcg
    13741 ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat atggtggatt ttctgccgaa
    13801 agtgaaaatt gagattgtcg taccggacga cattgtcgat acctgtgtcg ataccattat
    13861 tcgcacggcg caaaccggca aaatcggtga cggtaaaatc ttcgtctttg acgtggctcg
    13921 ggtcattcgc atccgtaccg gtgaagagga cgacgcggca atttaaaccg cgtcaagggt
    13981 tgccggatgt ttccatccgg caacatcaaa ttacagcacc ttatgcgggc caaagcattc
    14041 gtaatgaatg ttttcctgct tcacgcccag atccactaac tgtttcgcgg taaactgcat
    14101 gaagccaacc gggccgcaga gatagaactg cattgtcgga tcgctgaacg caccttccag
    14161 tttgctcaaa tccatcagac cttcgctatc aaactgacct ttagcgcgat cggcttcgct
    14221 cggctgacga taccaggtgt gcgcggtaaa gcgcggcagt gactgcccca gttccttaac
    14281 ttcatcagca aaggcgtgaa cttcgccatt ttctgccgca tggaaccagt tcacttgtgc
    14341 tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc attggcgttt gaccaacacc
    14401 ggtagagatt aacgtcactg gtgtgtcatc tgcgacagcc ataaagaaat cacctgccgg
    14461 agcgaccagt ttcacgacat cgccaacatt ggcgtgattg tgcaaccagt tggatacctg
    14521 cccaccctct tcgcgtttca ccgcgatacg atagcctttg ccatccggtt tgcgagtcaa
    14581 agagtactga cgaatttcct gatgcgggaa accttccggc ttcagccaga cgccgagata
    14641 ttgccccgga cggtattctg ccactgcgcc accgtcgacc ggctccagtt cgaagctggt
    14701 gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa tcgcgagtac cttcccaacc
    14761 accggctttg ctggcgtttt cgttatagat ttccgcctcg cgattgataa atacattagc
    14821 cagtacacca taggctttac cccacgcgtc cagcacttcc tgccccgggc tgaacatttc
    14881 gtccagcgtt gccaacaggt gttcaccgac gatgttgtac tgttccggtt tgatctggaa
    14941 gctggtgtgc ttctgcgcga ttttttctac cgctggcagc agcgcaggca ggttttcaat
    15001 attactggcg taggcggcaa tagcgttaaa cagggcttca cgttgatcgc cattacgctg
    15061 gttactcatg ttaaaaattt ctttgagttc tgggttatga gtaaacatac ggtcgtagaa
    15121 atgggcggtt aactttggcc ccgtttccac cagtaaaggg atggtggctt ttactgtagc
    15181 gatggtttga gcgtcaagca tatggtcttc cttttttgca tcttaattga tgtatctcaa
    15241 atgcatctta taaaaaatag ccctgcaatg taaatggttc tttggtgttt ttcagaaaga
    15301 atgtgatgaa gtgaaaaatt tgcatcacaa acctgaaaag aaatccgttt ccggttgcaa
    15361 gctctttatt ctccaaagcc ttgcgtagcc tgaaggtaat cgtttgcgta aattcctttg
    15421 tcaagacctg ttatcgcaca atgattcggt tatactgttc gccgttgtcc aacaggaccg
    15481 cctataaagg ccaaaaattt tattgttagc tgagtcagga gatgcggatg ttaaagcgtg
    15541 aaatgaacat tgccgattat gatgccgaac tgtggcaggc tatggagcag gaaaaagtac
    15601 gtcaggaaga gcacatcgaa ctgatcgcct ccgaaaacta caccagcccg cgcgtaatgc
    15661 aggcgcaggg ttctcagctg accaacaaat atgctgaagg ttatccgggc aaacgctact
    15721 acggcggttg cgagtatgtt gatatcgttg aacaactggc gatcgatcgt gcgaaagaac
    15781 tgttcggcgc tgactacgct aacgtccagc cgcactccgg ctcccaggct aactttgcgg
    15841 tctacaccgc gctgctggaa ccaggtgata ccgttctggg tatgaacctg gcgcatggcg
    15901 gtcacctgac tcacggttct ccggttaact tctccggtaa actgtacaac atcgttcctt
    15961 acggtatcga tgctaccggt catatcgatt acgccgatct ggaaaaacaa gccaaagaac
    16021 acaagccgaa aatgattatc ggtggtttct ctgcatattc cggcgtggtt gactgggcga
    16081 aaatgcgtga aatcgctgac agcatcggtg cttatctgtt cgttgatatg gcgcacgttg
    16141 cgggcctggt tgctgctggc gtctacccga acccggttcc tcatgctcac gttgttacta
    16201 ccaccactca caaaaccctg gcgggtccgc gcggcggcct gatcttggcg aaaggtggta
    16261 gcgaagagct gtacaaaaaa ctgaactctg ccgttttccc tggtggtcag ggcggtccgt
    16321 tgatgcacgt aatcgccggt aaagcggttg ctctgaaaga agcgatggag cctgagttca
    16381 aaacttacca gcagcaggtc gcgaaaaacg ctaaagcgat ggtagaagtg ttcctcgagc
    16441 gcggctacaa agtggtttcc ggcggcactg ataaccacct gttcctggtt gatctggttg
    16501 ataaaaacct gaccggtaaa gaagcagacg ccgctctggg ccgtgctaac atcaccgtca
    16561 acaaaaacag cgtaccgaac gatccgaaga gcccgtttgt gacctccggt attcgtgtag
    16621 gtactccggc gattacccgt cgcggcttta aagaagccga agcgaaagaa ctggctggct
    16681 ggatgtgtga cgtgctggac agcatcaatg atgaagccgt tatcgagcgc atcaaaggta
    16741 aagttctcga catctgcgca cgttacccgg tttacgcata agcgaaacgg tgatttgctg
    16801 acaatgtgct cgttgttcat gttggatgcg gcatgaacac cttctcaggc ctacaaaatc
    16861 ttgcaaattc aatatattgc aatctccgtg taggcctgat aagcgtagcg catcaggcaa
    16921 tttttcgttt atgatcatca aggcttcctt cgggaagcct ttctacgtta tcgcgccatc
    16981 aaatctgtcg taactgcgcc tcaacataca aatagccaat tcccagcacc tgttgtgcgc
    17041 ggcttaattg cccgaagcca atttgcgtcg ctttcacgct cggtccttcg tcgcggtcga
    17101 acgggttaaa tcctgtctgg cgaataatag tattaagcca gttctcacca aaggcacaac
    17161 tgcgaccgta caaccaaatc tgattaatat tgaggatgtt gaggaagtta tacagactca
    17221 aaccaatggc attggcagag cgatcaaccc agctggtgat ccacggttct ccactctgcc
    17281 aggcagcgat taactgcgcg gttgtcagtt tttcaggctc aagctgagta ttaaccggtt
    17341 gtgattttag ccatacccgc gcctgttttt ttaatgcact taacgaggcg acggtttcca
    17401 ggctgccgta acgtccacag tcgcagacga cgccatccgg attaacgatg gtgtgaccaa
    17461 tctgtccgct accatacaaa ctgccgcgat aaatttgctc gttaatcacg aacgacgagc
    17521 caatgccgta atcaacgttg atcacgcaga aatcccgttc ctgcgaatta ttttgccatt
    17581 tctccgccag cgccagcatc acgcagtcat tatcgaccag cacccgaatg cccagcttct
    17641 cttccagcag atacttcacc tcaactggcg ttgtccacgg cgcttgcggc atggtttgcg
    17701 atacgccggt caccggatca acctgcccgt ggattgccag cgccagattg atggtgcggt
    17761 ccggccacaa tttacggtgg cgatgccagc atttttcgat ttcggacagc agcgcctgcg
    17821 gcgtcggtac atcaatctgt aaatactcaa attcgccttt cggacttaaa caagcattag
    17881 caacctgaca ctcaatactg gtgggcgtca cgttcaggca cagcgtccag tcaccttccg
    17941 gcgcaatcag ccatgtaccg ctactatgcc cgcgcgtctg gctttcatcg tcaatattca
    18001 ccacccgttt ttcgctttcc agttcctgca aaatattact gactgccgga atcgaaattt
    18061 gcgccagccg ggccagcgtt gatttattgg cgcgcttttg ccggtacagc agttccagaa
    18121 tcacgcattt gttatggtgg agaatctgtt gattgttaat gcaggctctc atcttcacta
    18181 aaccttgttt agttaattgc gtgattattc cgcgaaaaca ttgccagatt gttcaataca
    18241 ctgccacaaa tcttttaatt cagtatgtct tgttaatatt gagggcacca tgactccagt
    18301 aaaagtgtgg caagagcgcg ttgagatccc gacctatgaa accgggccgc aggatataca
    18361 tcccatgttc ctggaaaatc gcgtttatca gggatcgtcc ggcgcggttt atccctacgg
    18421 cgtgaccgat acgctgagcg agcagaaaac cctgaaatcc tagcaggcgg tgtggctgga
    18481 aaacgactac atcaaagtga tgatcctgcc ggaactgggc ggtcgggtgc atcgcgcatg
    18541 ggataaagtg aaacagcgcg attttgtcta tcacaatgaa gtcattaaac ctgcgctggt
    18601 ggggatgctg ggaccgtgga tctccggcgg gattgagttt aactggccgc aacaccatcg
    18661 cccgaccacc tttatgcccg ttgatttcac cctcgaagcc catgaagacg gtgcacagac
    18721 ggtgtgggtc ggcgaaacgg agccgatgca tggtttacag gtgatgacag gtttcaccct
    18781 gcgccctgac cgggcggcac tggaaatcgc cagccgcgtc tataacggca acgccacgcc
    18841 gcgtcatttc ttgtggtggg ccaacccggc agtgaaaggg ggtgaagggc atcagagcgt
    18901 tttcccaccg gatgtgactg cggtgtttga tcacggcaaa cgggccgtct ccgctttccc
    18961 catcgccacc ggcacttact acaaagtgga ctactccgcc ggagtggaca tttctcgcta
    19021 taaaaatgtg cctgttccaa cctcatatat ggccgaaaaa tcgcagtacg attttgtcgg
    19081 cgcgtggtgt cacgatgaag atggcggttt gctgcacgtt gccaaccacc atattgcgcc
    19141 aggtaaaaaa cagtggagtt ggggacacag tgaatttggc caggcgtggg ataagagtct
    19201 gaccgacaat aacggcccgt atatcgaact gatgaccggt atttttgccg ataaccagcc
    19261 tgattttacc tggcttgatg cttacgaaga gaagcgtttc gagcagtatt tcctgcctta
    19321 tcattctctg ggcatggtgc aaaatgcctc ccgcgatgcg gtgataaaac tccagcgtag
    19381 tgagcggggg attgagtggg ggctgtatgc catctctccg ttgaacggat accgtctggc
    19441 gatccgcgaa atcggcaaat gcaacgcgtt gctcgatgat gccgtggccc tgacaccagc
    19501 gaccgccatc cagggcgtgt tgcacggtat caatcctgac agactgacca ttgaactctc
    19561 cgatgccgac ggcaatattg tactgagtta tcaggaacat cagccgcagg agttgccgtt
    19621 gccggacgtc gccaaagcgc cactggcagc acaagacatt accagtacag atgaagcctg
    19681 gtttatcggt cagcatctgg agcaatatca tcacgccagc cgttcaccgt tcgattacta
    19741 cctgcgcggc gtggcgctgg acccgctgga ttatcgctgt aacctggcgc tggcgatgct
    19801 ggaatataac cgcgcagatt tcccgcaagc ggtggcgtat gccagtcagg cgctgaaacg
    19861 cgcacatgcg ctgaacaaaa acccgcagtg tggacaagca agtttgattc gcgccagtgc
    19921 ttacgaacgt cagggacaat atcaacaagc cgaagaggat ttctggcgtg cggtctggag
    19981 cggcaatagt aaagcaggcg gctattatgg tctggcacga ctggcggcgc gtaatgataa
    20041 cttcgacgcg ggtctggatt tttgccaaca aagtcttcgt gcctgcccaa ccaatcagga
    20101 agtgctttgc ttgcataatc tgctgctggt gttaagtggt cgtcaggaca acgcgcgttt
    20161 gcagcgcgag aaactgctgc gtgattatcc gctgaacgcc actctgtggt ggctgaactg
    20221 gttcgatggt cgtagcgaat cagccctcgc gcagtggcgc ggtctgtgtc agggacgcga
    20281 cgttaacgct ctgatgaccg ccgggcaact gattaactgg ggaatgccca ccctggcggc
    20341 agagatgctg aacgcactgg actgccagcg cacgctgccg ctttacctgc aagccagctt
    20401 gctgccgaaa gccgaacgtg gcgaactggt cgcaaaagcc attgatgtct tcccgcagtt
    20461 tgtccgtttc ccgaatacgc tggaagaagt ggcggcgctg gagagtattg aagagtgctg
    20521 gtttgctcgc catttactgg cctgcttcta ctacaacaaa cgtagctaca acaaagccat
    20581 tgccttatgg caacgttgcg tagagatgtc gccggagttt gccgacggct ggcgcgggtt
    20641 agcgatccat gcgtggaata agaaacacga ttatgagctg gccgcgcgtt atcttgataa
    20701 tgcttatcag cttgcgccgc aggatgcacg tctgcttttc gaacgggatt tgctggataa
    20761 gttaagtgga gccacaccgg agaaacgact ggcgcgtctg gaaaataatc tggaaattgc
    20821 gctgaaacgc gacgacatga ccgcagaact gctcaatttg tggcatctca cgggtcaggc
    20881 agacaaagcg gcggacattc tcgccacgcg taaattctac ccgtgggaag gcggggaagg
    20941 gaaggtcacc agtcagttta tcctcaacca gttattacgc gcctggcagc atcttgatgc
    21001 cagagagccg cagcaggcca gcgaactgct tcatgccgcg ctgtattatc cggagaattt
    21061 aagcgaaggc cgtttaccgg ggcaaactga taacgacatc tggttctggc aggcgatatg
    21121 cgccaacgcg cagggcgatg aaactgaagc gatgcgttgt ttacgtctgg cggcgaccgg
    21181 cgatcgcacc attaacatcc acagttatta caacgatcag ccggttgatt atctcttctg
    21241 gcaaggaatg gcgctgcgac tgctgggtga acagcaaacc gcacagcaac tgtttagtga
    21301 aatgaaacag tgggcgcaag agatggcgaa aaccagtatt gaggcggatt tctttgctgt
    21361 ttcacaacct gacctgttat cgctgtatgg cgatttacaa cagcagcata aagaaaaatg
    21421 cctgatggtg gcgatgctgg cgtccgcggg actcggggag gttgcgcaat atgagtctgc
    21481 tcgcgctgaa ttgacggcga ttaatccggc ctggccgaaa gcggcattat tcaccactgt
    21541 gatgcctttt atttttaact acgttcacta aaccctatta actgtaatat ttgagcggca
    21601 cgcgctgccg ctcatcacaa aataattaga ataagccgtt gttaataaaa accctacagg
    21661 aggcactatg cctaaaaaaa tgagaacaac ccgtaatcta ttattaatgg ctacgctatt
    21721 aggtagcgca ctatttgcca gggctgcgga taaagaaatg accattggtg caatatacct
    21781 tgatacccag ggatattacg ctggagtgcg tcagggcgtt caggatgcgg caaaagattc
    21841 ttcagtacag gtacagttaa ttgaaactaa cgcccagggg gatatttcga aagaaagtac
    21901 ctttgttgat accctcgtgg cgcgtaatgt cgatgccatt attttatcgg cagtgtctga
    21961 aaatggcagt agccgtaccg ttcgtcgcgc cagtgaagcg ggtattccgg tgatttgcta
    22021 caacacctgt attaatcaaa agggtgtcga taaatatgtc tcggcgtatc tggtcggcga
    22081 tccactggaa tttggtaaaa aactgggtaa cgctgccgcc gattatttta ttgccaataa
    22141 aattgaccag ccgaaaattg ccgtcatcaa ttgcgaagcc tttgaagttt gtgtgcagcg
    22201 acgtaaagga tttgaagaag tattaaaatc ccgcgttccc ggcgcgcaaa ttgtcgctaa
    22261 ccaggaaggg actgttttag ataaagcgat ttccgttggt gaaaaactga ttatctccac
    22321 gccggatctc aacgccatta tgggggagtc gggcggtgcg acactcggcg cggtaaaagc
    22381 ggtacgtaat caaaatcagg ccggaaaaat tgctgttttc ggttcggata tgacaaccga
    22441 aattgctcag gagctggaaa acaatcaggt gctgaaagcg gtagtggata tttccggtaa
    22501 gaaaatgggc aatgctgttt tcgcgcaaac attgaaggtt atcaataaac aagccgacgg
    22561 tgaaaaagtg attcaggtgc caatcgatct ctataccaaa acggaagatg gtaaacagtg
    22621 gctggcaacg cacgttgatg gtctgcccta atcgctgatt cgcagaggtc cctatgttca
    22681 cggcaacaga ggcagtcccg gtagcaaaag tggtggcagg aaataagcgt taccccggcg
    22741 tcgttgcgct ggataacgtt aacttcacgc tcaataaagg cgaagttcgt gcgctgttag
    22801 gtaaaaacgg cgcgggcaaa tcgactctca ttcgaatgct taccggcagc gaacgcccgg
    22861 atagcggtga tatctggatt ggcgagacgc gactggaagg tgacgaagct acgttgactc
    22921 gccgtgccgc tgaactgggg gttcgtgcgg tttatcagga attaagtctg gtggaagggc
    22981 tgacggtggc agaaaacctc tgccttggtc aatggcctcg ccgcaacggc atgattgatt
    23041 acctgcaaat ggcgcaggat gcccaacgtt gcttacaggc gctgggcgtt gacgttagtc
    23101 ctgaacaact tgtttcaacg ctaagcccgg cgcaaaagca actggtggaa attgcacggg
    23161 tgatgaaggg cgagccgcgc gtggtcattc ttgatgaacc caccagctcg cttgccagtg
    23221 cagaagttga actggtgatc agtgcggtga aaaagatgtc ggcactgggc gtggcggtga
    23281 tttatgtcag ccaccggatg gaagaaatcc gccgcattgc ctcctatgcc accgttatgc
    23341 gcgatggtca ggtggcgggc gatgtgatgc tcgaaaacac ctccacgcat catattgtgt
    23401 cgctaatgct agggcgtgat cacgttgata ttgcgccggt tgcccctcag gaaattgtgg
    23461 atcaggccgt actggaagtc cgtgcgttac gccataagcc caagctggag gatatcagct
    23521 ttacgctacg tcgtggcgaa gtgcttggca ttgctggcct gctgggggca gggcgcagtg
    23581 aattgctgaa ggcgattgtt gggctggaga cgtatgaaca gggcgaaatt gttatcaacg
    23641 gcgagaaaat catgcgcccc gattacggcg acatgctgaa acgcggcatt ggctatacgc
    23701 cagaaaaccg caaagaagcg gggatcattc cctggctagg cgttgacgaa aatacagtgc
    23761 tgaccaatcg gcaaaaaatc agcaccaacg gtgtgctgca atggtccacc atccgccgcc
    23821 tgaccgaaga ggtgatgcag cggatgacgg tcaaggctgc cagtagcgaa acacccatcg
    23881 gcacgctttc tggtggcaat cagcaaaaag tggtgatcgg tcgttgggtc tatgccgcca
    23941 gccagatttt gttgctcgac gagccaacgc gcggcgtcga tatcgaagcc aaacagcaga
    24001 tttaccgtat tgtccgtgag ctggctgccg aaggaaaaag cgtggtgttt atctccagtg
    24061 aagtggagga attgccgctg gtgtgtgacc gcatcctgtt attacagcac ggcacgttct
    24121 cgcaggagtt tcactcgccg gtcaatgtgg atgagctgat gtccgccatt ctgtctgtac
    24181 actgatgaat tttaccgagg aacctgaaga tgtatgcttc gtcattacca ttgccgcagg
    24241 gcaagagcgt ctcgctcaaa caatttgtca gtcgccatat taatgagatc ggtttgctgg
    24301 tggtgattgc cattctttat ctggtcttct ccctgaacgc gcctggcttt atctcattga
    24361 ataaccagat gaacgtgctg cgcgatgccg ccaccattgg gattgccgcc tgggcgatga
    24421 cgctgattat tatctccggt gaaattgatg tcagcgttgg gccgatggtg gcttttgtct
    24481 cggtgtgcct ggcatttttg ctgcaatttg aagttccgtt ggcgattgct tgtctgctgg
    24541 tgttgctgtt aggcgcgctg atgggaacgc tcgccggggt gctgcgcggc gtgtttaacg
    24601 taccaagttt cgttgccaca ctggggctgt ggagcgctct gcgcggaatg gggctgttta
    24661 tgaccaacgc actgccagtg ccgattgacg aaaacgaggt gctggactgg ctgggcggac
    24721 aatttctcgg tgtgccagta tccgcgctga tcatgatggt gttgtttgcg ctgtttgtgt
    24781 tcattagccg aaaaaccgcc ttcgggcgct cggtttttgc cgttggcggt aatgccacgg
    24841 cggcgcagtt gtgcggtatt aacgttcgtc gagtgcgcat tcttatcttt accctttcgg
    24901 gattattagc ggcggtgacc ggcattttgt tggcggcacg cctcggttcc ggtaacgcag
    24961 gtgccgcaaa cggtctggag tttgacgtca tcgccgcggt cgtggtcggc ggtacggcac
    25021 tttccggcgg tcgcggttcc ctgttcggta cattgcttgg tgtgctggtg attacgctaa
    25081 tcggtaacgg tctggtgctg ctcggtatta actccttttt ccagcaggtg gtacgcggcg
    25141 tcatcatcgt ggtggcggtg ctggcgaata tcttgctgac ccagcgaagc agtaaagcga
    25201 aacgctaaac cttaacgccc ctggcgcggg tgcggcaggg atgaaaatta atcgtattgt
    25261 taatgaggga tttatgaaaa cgatgctggc tgcttattta ctaggaaatt caaccgtcga
    25321 tctgcgggaa gttgcggtgc cgacgccggg gattaaccag gtactgatca aaatgaaatc
    25381 ctccgggatt tgcggaagcg atgtccacta tatctatcat caacaccgtg ccacggcggc
    25441 ggcacccgat aaaccgttat accagggatt tatcaacggt catgaaccgt gcgggcagat
    25501 tgtggcgatg gggcaaggct gccgccattt taaagagggc gaccgcgtgc tggtgtatca
    25561 catttctggc tgtggttttt gcccgaactg ccgccgcggc tttcctatct cttgtactgg
    25621 caaaggaaaa gcggcttacg gctggcagcg tgacggcggt catgccgaat atctgctggc
    25681 ggaagaaaaa gatctgatcc tcctgccgga tgcgctgagc tacgaagatg gtgcgtttat
    25741 cagttgcggc gttggcacgg cctatgaagg gattttgcgc ggcgaagttt ccggcagcga
    25801 taacgtgctg gtggtcggtc tggggccggt cggcatgatg gcgatgatgc tggcgaaagg
    25861 tcgcggtgca aaacggatca tcggcgttga tatgctgccg gaacgtctgg cgatggcaaa
    25921 acagttaggg gggatggatc acggctattt agcaaccacc gaaggtctgc cgcagataat
    25981 tgctgaactt acccacggtg gcgcggatgt tgcgctcgat tgttccggta atgccgcagg
    26041 tcgcttgctg gcactgcaat ccactgctga ctgggggcgg gtggtttaca ttggtgaaac
    26101 cggaaaagtg gaattcgagg tcagcgctga tctgatgcac catcaacggc ggattatcgg
    26161 ctcctgggtg accagtctgt tccatatgga aaaatgcgcc cacgatttga ctgactggaa
    26221 gctgtggccg cgtaacgcca ttacccatcg cttctcgcta gaacaggcag gtgatgccta
    26281 tgtgctgatg gcgagcggca aatgcgggaa agttgtgatt aacttcccgg attaaggagt
    26341 tagcggatga ccatctatac cttatcccac gggtcgctaa agctggacgt ttctgaccag
    26401 ggcggcgtaa tcgaaggctt ctggcgcgat acgacgccgc tcttacgtcc tggtaaaaaa
    26461 agcggtgtgg caaccgatgc ctcatgtttt ccgctggtgc catttgcaaa ccgggtgagc
    26521 ggcaaccgtt ttgtctggca ggggcgtgag tatcaactgc aaccgaatgt tgagtgggat
    26581 gcgcactatc tacacggcga tggctggctg ggcgagtggc aatgtgtctc gcacagcgat
    26641 gatagtttgt gtctggtgta tgaacatcgc agcggtgtct atcactatcg ggtaagtcag
    26701 gcgtttcatt taacggcgga tacgctgacg gtgacgctct ctgtcaccaa tcaaggggca
    26761 gagacgctgc catttggtac cggctggcat ccttattttc cgttgtcacc acaaacgcgg
    26821 attcaggcgc aggcgagcgg ttactggctg gaacgggagc agtggctggc gggtgagttt
    26881 tgcgagcagc taccgcagga actggatttt aaccagctcg cgccgttgcc gcgtcagtgg
    26941 gtgaacaatg gttttgccgg atggaatggt caggcccgca tcgagcagcc gcaggaaggt
    27001 tatgccatca tcatggaaac gacgccacct gcaccgtgtt atttcatctt tgtttccgac
    27061 cctgcgtttg ataaaggata tgcgtttgat ttcttctgtc tggaaccgat gagccatgcg
    27121 ccggatgatc atcatcgccc ggaagggggt gatctcattg cgctagcacc aggggaatca
    27181 acaacctcag agatgtcgtt gcgggtagag tggctgtaat ttataaaatg ccggataagg
    27241 cgttcacgcc gcatccggca tctttcctaa cgccgatcga gagaaattgc ccccggtccg
    27301 gtaattgcca atagcaagaa cgcgccagcg atactgacat tcttccagaa attaatcata
    27361 tttggcccaa ccgcatcgcc ggtcatatcc cagtaatggt gaccaatcac cgccgtaccc
    27421 agcgtgtaga aaataaacag caccgccagc ggacgggtga aaaagccaag cacgattaat
    27481 atcgcggcgg gcacttccat aactaccgca ataatcgctg ccagcatcgg cattggcgcg
    27541 cccaacgagg ccatatattg gaccgtaccg tcaaagccca tcattttggg aaaaccaaaa
    27601 ataatgaaaa ttaagaccac ggcgatacgg gcaattaata acaaaacggg gcgggcagct
    27661 ccaaaatcaa aataacgtag tgtgttcata accaatccct ttgcaagata atgtgattta
    27721 aacgtaatac acactcttgc acctcgccac gccccaggca ttgagatatt cgctaattat
    27781 cctgattaca gtgatttaag cgcgatgttc tcatctatca gcagtttcgc atcaaacgtt
    27841 ttgccgctct ggatccattt gcgaattggg cgaatctcac ggccctgatt cagcgtgacc
    27901 gcaccgataa gcacgccgtt ttgcagatta aaccaaatcg ccttctgagt ttccgggttg
    27961 ccacgacaaa gccagtcatc gccacgcata tcgccaataa actgtaagtt atcactgtac
    28021 tgatcgctcc agaaccacgg cggcggcagt agcggtagcg gtagccctaa cattgcggca
    28081 gcggcaattt gcgcctggtt attggcgttt tcccagcttt cgcagcggtg tagtgcacca
    28141 ttatcaagac gagtgattgc cacatcgcca ccggcaaaga tcgcgggatc gcaggtgcgg
    28201 caagcctcat caatgacaat gccattggca gtatcaaggt tggcctcgcg agccagttgc
    28261 tcgttggcgc tgataccaat accgtaaatc accacatcag cctgaagcgt ttccccactt
    28321 tgcagcgtca gttctacttt ttcaccatcg accacatgtt caatggcatt attgagcaga
    28381 atgcgcacac cagcctgctg atggcgctgt aaaagatagc gttgcacggg cggtggtgca
    28441 ttacggccca tgacggttgc cgccagttca atcactgtca ccttacatct gcgctgcgtg
    28501 gcgctggcag ccagttccag accaatagtt ccggcaccga caatcacgac tgaccgttcg
    28561 ggctgcagaa cttctcgcag tctggcggca tcaccggcat ggtgtagggt aaagcagcgt
    28621 tctcccagtg catcaagcaa cggcagcggt cgagctgccg cgccggttgc tataaaaagc
    28681 tgatcccagt gccagctttc gccgttggtt aacactaact ctcgtgtgtc gcgacccagc
    28741 gttttgatgg ttacaccgga atgcagatga acattgtttt cctgccacca gttagcgggt
    28801 aacacctgct gtaactgcgg ggaatcttcc agcaacatgg atttcgagag tggaggtcgt
    28861 tcataaggaa gatgtcgctc atcggaaaac agatgcagct caccggtgaa cccttgctgg
    28921 cgtagcgagg ccgcagccat tgccgccgct tgcccgccac cgacaataat gatcgttttt
    28981 tctttcatcg tgcttcctta aagatccagc ccagccgcta cgtggcgaat gccgcgaatc
    29041 gctaaacccg catcagcgtt gatcatcaca ccgcttaatg cgcgattatt gcgccgcgat
    29101 gccaacatca catacggccc cgtaaaatcc gccggttgcg ggaaaaattg cagcggtaaa
    29161 atggcggcaa ttttctccgg cgtcagagac tgcattatcg aggtttcact ttgcccgagc
    29221 gcctgtgggc cgcgcaggtc gctggccata ccacacgggc cgacgccatt cacccgcact
    29281 ttcggtgcca gttcataagc cagttggcga ataagtccgg ttgcggcatg tttactggtg
    29341 gtgtacagcg ggccaccgcc gccaggatac caggcggcat ttgacagtgt gaaaatcatg
    29401 ctgccttcac tggcgattaa cgccggagcg caggcttttg cgcccagcag gtaaccgagt
    29461 acgttgacgt taaacagctc gtggaagccg gtttcgagcg tctctgcggg agtattaacc
    29521 agtgaggcat tgtgatccca gatgcctgca ttgccgataa aacaatccag cttgccggaa
    29581 cgagtcagga tctgatcgac cgcgcgttga taatcggcat aacaggtcac gttaccttcc
    29641 accgccagaa tatgttcgcc aaatcgctga cgcagactgg cgacttttgc cgccgacagt
    29701 tccagcgtgg caacttgcgc gccttcttcg ataaatcgct cgaccagcgc cagccctaat
    29761 cccgatccgc cgccggtaat aaaaatggac tcgttatgca gatcgctcat ggctgcgcct
    29821 ccggtaagtc gatgaaaatg tcaccacctt caacgtgtac tggataagtg gtgagcggat
    29881 cggtggcggg caggcataac gctttccccg ttttcaggca aaaactggcg gcgtgtagcg
    29941 ggcactccac cgtggcgtca tcttccagat acccttctga catcgacgca ttaccatggc
    30001 tgcaacgatc gttaatggca taaaactcgc cgcccacgtt gaacaaggcg atgacgggcg
    30061 aggtatctat ccggagagcc tcaccctccg gcacatccgc aacgggacac gcataaattc
    30121 gattcatcag aacagtacac tcaggttatg ggaagtgatc accgcttgat ccaggacgat
    30181 atcccgttcc agcaagcgcc agttgtcatc ttccagacgg cgaactttgt cgaaacgcgt
    30241 tccaacatag aatgtttcat cgcgctcttt ttgtgcccga taaagtagat aattcaccct
    30301 cacggcaaat acgtttggga tgtcggtttc gcttacctgg cagttgctga ttaagtgacg
    30361 agtgcgtgac ggcggctctt ctgcccaggc aatgcccgtt tccagacggg cgattcgccg
    30421 ctccagctgg tctttggtgt cattgaaaat ccaggtcgtc ggcggctgga cgcctttgcg
    30481 acggtcgcgt gtttgcgcgt taaccgtggt gcgcatggtg taacgaatct cttcgtcgag
    30541 ctgtgccaac cagtcacgaa atttccagtc atccagcaga ctggcttcgt gaaagagaaa
    30601 ctggctaatg cggtgatgta actccagtga aacttgcgca ctcatttcat cacctcctgc
    30661 tggtaagcgg cggttttatc gagcacttcc tgccagcttt cgctactcag aagatcggcc
    30721 cagcgttggt acattccacg agcggcagtt tctgaaaaga tatagttagt aatgccagga
    30781 atgccgtcgt cgcggcgctt ttcctgacca agccccattt ccagacacag tttgctgttg
    30841 cgggcgcggt ggcctttaag caatttctgg atttcacacc agttctccga gtcatcctgc
    30901 tcgagaaaac cagcaggacc aaaagcacga gtggcgctgt tttcaaaagc ggctttaact
    30961 tcatcggagg cggctttgtc agtaatacag aacgcccaca cttcaacttg atcagggccg
    31021 cgcggatgcc agacgcggag tgtggcagtg ccgtttagcc atgaaagcgt ggggaatata
    31081 ttgttatgac ccgccaggcg cagggcgcga acttcaccta aacgttgttc tgcttcggca
    31141 taggtttcgc ggtaatagct tgaaactgcg ccatcgaccc agacattagc atccggtttt
    31201 tcagtaaaga agaaaccgct accgtgaccg tcctgaccaa attgcagcgc atctttggcg
    31261 gtttcccaca ccgggcgggc ggtttgtcca tcaccgaggc gcttatcgct gccatcatct
    31321 ttcgccccta atacctgaac ggcagaagca tggctgaaca gagcatgata ctggtcactg
    31381 gcgaactgct ctgccgggaa tttccagtta cagttgatca cccacttttg tacgccgccg
    31441 acaatttcgg tgccgccttc gcgacgatcc agcatgccat ccagatacca ggcaatgtca
    31501 cccaggtaat catgcaggcc cggtgcgctg gtatcccagt tgccaaaaat cagtccttta
    31561 taactctcca cacaaggaac ttcgtttagc ccccagtggg atttacacaa cccttgtggg
    31621 taggcgcgag gttccagcgg tacatcgatc aactcgccgt taatgccata agaccagccg
    31681 tgatacgggc aggtaaaggc gcgagtgttg ccacaatctg cataactcac acgcatggcc
    31741 cggtggcggc attggttgag aaacgccttg atgctgccgt ctttctggcg caccacgaca
    31801 actgcatctt ctcccatgta ggtgttaaag aaatcaccgg gttttgggat ctggctttcg
    31861 tgggcgagaa atagccagca acgaccgaaa atacgctcaa gctccagttg gtaaatgtcc
    31921 gggtcggtat aaatacgcgg agtgacccga ccgttttggg tgtcaatcag ttggtaaatg
    31981 ttcaaatctg agggtgtggt catatttttt aatcctgaaa gataagatgt attgaccttc
    32041 tatttttgtg ccataaggat gttgctggtt ggttgctaat atgtgaaata gttaatttct
    32101 accagctgag acgttttttc gataacaagg ggaaggtgat ggaactacgg catttacgct
    32161 atttcgtcgc agtggcgcag gcactgaact ttacccgtgc ggcggaaaaa ctgcatacct
    32221 cacagccttc gttaagcagc cagatccgcg atcttgaaaa ctgtgtcggt gttccgttac
    32281 tggtgaggga taagcgcaaa gtcgcgttga cggcggcggg agaatgtttt ctccaggacg
    32341 cgctggcaat cctcgaacaa gcggaaaatg ccaaattacg ggcgcggaaa attgttcagg
    32401 aagacagaca attaaccatt ggctttgtgc catcggcgga agtgaattta ttgccaaaag
    32461 tattaccgat gtttcgtctc agacagccag acaccttgat tgagctggtg agtttaatca
    32521 ccacgcaaca ggaggaaaaa attcgccgtg gtgaactcga cgtcggctta atgcgccatc
    32581 ctgtttatag cccggagatt gattatctgg agctttttga cgaaccatta gtggtggtgt
    32641 taccggttga tcacccttta gcacatgaaa aagagatcac cgccgcgcaa ctggatggcg
    32701 tgaatttcgt cagtaccgat ccggcgtatt ccggttcgct tgcgccgatc gttaaagcgt
    32761 ggtttgcgca agaaaacagc cagccaaata tcgtccaggt ggcaacgaat attctggtga
    32821 ccatgaatct ggtgggcatg gggctgggcg tcactttgat acccggttat atgaataatt
    32881 ttaataccgg gcaggttgtt tttcgtccca ttgccggaaa tgtgccttcc attgctttac
    32941 tgatggcgtg gaagaaaggg gagatgaagc tggcattacg cgatttcatc gccattgtgc
    33001 aggaacgttt ggcaagcgta acggcataat tacgcattat ttttgcaaca tgcgtcgcgg
    33061 atcgcagttt tttagtgctt gtgaactggc gttacagaca tcgggaagca ggctcgaata
    33121 ttcttatttt tcaacttgca ccaacattcg attaacccca gactaacgcc tcctgacggg
    33181 agggactcat ggttttgcaa tccacgcgct ggttggcgct cggctatttc acatactttt
    33241 ttagttacgg cattttttta cctttctgga gcgtctggct taaagggatt ggtttaacgc
    33301 cagaaaccat cggcctgtta ttgggggcag gtctggttgc ccgttttctc gggagtttgc
    33361 tcatcgcgcc ccgcgtcagc gatccttccc gcctgatttc cgccttgcgc gtgctggcac
    33421 tgctgccact tctctttgct gtcgccttct gggcgggggc gcacgtagcg tggctgatgc
    33481 tggtgatgat tggctttaac ctctttttct caccgctggt accgttgacc gatgcactgg
    33541 cgaatacgtg gcaaaagcag ttcccgcttg attacggcaa agtgcgactg tggggctcgg
    33601 tggcgtttgt cattggctcg gcgctgacgg gcaaactggt cactatgttt gattatcggg
    33661 tgattctcgt gctgttgacg ttgggcgtgg catccatgct gctcggcttt ctcatccgtc
    33721 cgacgattca gccacaaggg gcaagccgcc agcaggagag caccggttgg tctgcgtggt
    33781 tggcgctggt tcgccagaac tggcgctttc tggcctgcgt ttgtttattg cagggggcac
    33841 atgcggccta ttacggtttt agcgccattt actggcaggc agctggctac tcggcctcgg
    33901 cggtggggta tttgtggtcg ctgggcgtgg tggcggaagt cattatcttt gcgctgagta
    33961 ataaactttt ccgccgttgt agtgctcgcg atatgctgtt gatctcggcg atttgcggcg
    34021 tagtgcgctg gggcattatg ggagcaacta cggcgttgcc gtggttgata gtggtgcaaa
    34081 ttctgcattg cggcaccttc acggtctgcc acctggccgc catgcgttat attgctgctc
    34141 gccaggttag cgaagtcatc cgtttacagg cggtttactc tgccgtcgcg atgggcggca
    34201 gtatcgctat catgaccgtt ttcgccggtt tcctgtatca atatctgggc cacggcgtgt
    34261 tctgggtaat ggcgctggtg gcgcttccgg caatgttttt gcgcccgaaa gttgttccct
    34321 catgctgatt cgagcatttt gcggatctgc tcctgttgat gtgttgtcag cgtcaggtcc
    34381 gtatagatca gcggtggtga aaagagcggt aacggcatgg tataaggcgc aataatcagt
    34441 gccgcgccac gcggagcgcc tgtctgcaaa aatgccttta ccgacatatg cttaatgttg
    34501 agcggcagta acgttagttc gcgtaactgc tgctcaatct gcgcttctcg ctcgctatca
    34561 ttcccggtca gtagaataat ctgtttttca tgcagatcgt tttcctgcat tagccaggca
    34621 ccgaaaatca ccgcgaccag accactttcc tcatcagaaa gatggacgcc gtattcactt
    34681 tcaaatccgg ccagcgccgc gcgggtggtg cgtaccaggc gtggatacag tctggcgaac
    34741 tcttccggca gggtattatc aataccgatg gcaaacaaac tgcgatttaa cgcctgggcg
    34801 aggtggatat aaagctgatc acataacccc tgttcatcat aaaaacgaac atttcccagc
    34861 tcacgaaaat ggtttaccag acgtttgata gactgacgca attgtctgtc ccgctgatgc
    34921 gcatcccgca gtggatcggg aacccgcaac atggaaaaaa gtaatgccat aaacagtggc
    34981 tcatcaggcg gtacatgctg gagcgcccga cgctgccagt ggcgtccaat ttcctgcgct
    35041 acctgaaatt caaggcagga ttccgcccag cgacgttgga gcggattaaa ctgaggcgta
    35101 attccagcgt ggtgctgcag caaacaatat tgcagataca gacacaggaa atgaatatcg
    35161 cgcgattcaa agcgtttttg cagccgtcgg gagcagagat tcactaacgc ttgtagattg
    35221 gtatcgtcgt aaaagttacg cgcaattccg cgccgcttca gttcactctt cagggcgggg
    35281 gtgaattggc tggtaataaa tgacggacac agacggaaac cacgtcgtag ccagtgaaat
    35341 aaacacaaac gttggttaag cactgtacct tcaacccggt agctaccgtc ataaccagtc
    35401 gtcagtgtga gttgatgata gcgcaggatc tcccgtcctg tttcgctgat atcaagactg
    35461 gcaatatcat catccacacc attaagctcg ctgaaggttg ccatggtggc ggtcaacccc
    35521 ggctgaaaga gcgtcagcaa gatctggcag cggcgctggg gagccgaaag gacagatggt
    35581 ggagcaagcg taggcatcat ctgtagatct cccataagca taattgataa gaatagctaa
    35641 agtttgctca gggaaatggc gaagggaatg ctaatcatca gaaatgttgg caagcgtcac
    35701 agaatttttc tcattttaaa ctgttctgac cagtggatta gcatgtctta aggtggtttt
    35761 gtgaatgatt atcgtgctag cgatcatggg ttagttctgc ggattcaacg cgctaaggat
    35821 aaagtccacc atctgttcgc gataaacatt gccgtgacga tcggaaaaag catcaatgtg
    35881 ttcgccatcc gggattagga tcaaccgttt tggctctttt gccaggctat acaacttttc
    35941 gctgtgctgc catgggataa cgtgatcggc tttaccgtga atgagtaaaa gcgggatcgg
    36001 gctgacgctg gcgatgtaat tttcgccgct gtaactctca tcaagtaagt agccactgcc
    36061 ggggatcatc tggttggcga tggttgcata agaggcaaat gtggagtcga ggatcaccgc
    36121 acgtatgcct tcacgatcac cctgaccaat aacagcaaga atattcgccc cgccaatact
    36181 ctgcccgaac agcaccagct gttgtgggtt tacatcactg cgatggcgca ccacattgat
    36241 ggcgctttgc gtatcgtcca gcaatccggc ctgggacgga gtgccttttg atttaccaaa
    36301 cccgcgataa tcaaacataa aaacgttgaa attacgctca ggtaaccaac tgaccagcgg
    36361 ccagtgggcg gacatatttc cggcattgcc gtgagcatga atgatggttg cgatggcgtt
    36421 gtcagcaggg cccgtcgaag aaggaataaa ccagccttgc agacgagtac cgtccttagc
    36481 cgtaaattcg acggattccg ccgaccaggg gtcgggaccg taaattttat cgtcaggata
    36541 atagaagaag ttgatggcaa cgcgcggcac taaataaacg cagaaggcaa caacaaagag
    36601 aataaacaga attttgggaa cgcgtttgtt cactggcagt gccataacct ttccttgcga
    36661 cttctttatc atccattaat cggggaaaga tggtgggaga gtgacgaaag aaagtaaagc
    36721 ggtgaggaag tatttcggaa tagcgcctga aaggcgaggg cgggtgagtg atatcacccg
    36781 cctgagtcat taatgcttca gagcgtcgct taactcgtca cgcatgttcg ccagtatggc
    36841 tttcacaaca cgcgggttac cagcaacgat gttaccggtc agcatatagt tatgaccacc
    36901 ggtgaagtcg ctgacgatgc cgcccgcttc acgaaccagc agctcgcctg ccgcgaaatc
    36961 ccacggacgc agaccgattt caaagaaacc gtcaacacga cccgcagcga cgtaagccag
    37021 atccagcgcc gcagaaccgg tacgacggaa gtctgcacat tcgttgaaca gcttgccgac
    37081 gatgttgatg taggtagtgg cgtactgttt tgctttgaac gggaagccgg tcgccagaat
    37141 ggtaccgtcg agatcgcgag cggtgctacc gcgcagacgg tagccgttca gctgtgcgcc
    37201 ttgaccgcga gtggcggtga acagttcgtt acgcatagga tcgtatacca cagcaacttc
    37261 ggtgcggcct ttgatacgaa cggcgataga taccgcgaag tgcggcagac gtttgataaa
    37321 gttggtagta ccatccagtg gatcgataac ccattgaaca tcctgatcag taccttcaag
    37381 ttcaccgctt tcttcggtga tgatggtgtg ctgtgggtaa gatttacgaa tcgtgtcgat
    37441 aatcaccgct tcggcagctt tatctacgtt ggtcacgaaa tcgttactgc ctttctggct
    37501 cgcttctaca gcgtccgggg tttcatagtt tttggcaact aaattacccg ccttgcgcgc
    37561 tgcgcgcacg gcgatgttca gcatcggatg catcggtctc tctcactgga tgttaaagaa
    37621 cgggaaaacg gcgcagagta tagcagcgag atcggcatct gtcttcgttt tatgataaga
    37681 tgcgtgaata atctttagat gaagaaagac aatgctgcaa aatattcgaa ttgtgctggt
    37741 ggagacgtca cacaccggca atatgggttc tgttgcccgt gccatgaaaa caatgggatt
    37801 aaccaatctg tggctggtta atccactggt gaaacccgac tcccaggcga ttgccctggc
    37861 tgcaggggcc agcgatgtga ttggtaatgc tcacatcgtc gatacgctcg acgaagcgtt
    37921 agccggttgt agcctggtgg tgggcaccag cgcacgctcc cgcacgctgc cgtggccgat
    37981 gctcgacccg cgcgaatgcg gtctgaaaag cgtcgctgaa gcggcaaata ccccggtggc
    38041 gctggtgttt ggtcgtgagc gcgtcggctt gaccaatgaa gagttgcaga aatgccatta
    38101 tcatgtcgcg attgcggcaa acccggaata cagttcgctg aacctggcaa tggcggttca
    38161 ggttattgcc tatgaagtgc gtatggcctg gctggcaact caggaaaatg gcgagcaggt
    38221 cgagcatgaa gagacgccgt atccgctggt cgatgatctg gagcgttttt atggtcatct
    38281 ggaacaaacg ctgctggcaa ccggttttat ccgtgaaaac catccggggc aggtgatgaa
    38341 taaattgcgc cgtctgttta cccgtgcgcg cccggaaagc caggaattga atatcctgcg
    38401 cgggattctg gcttctattg agcagcagaa taaaggtaac aaggccgaat aacagtggtt
    38461 gcctgatgcg acgcttaacg cgtcttatca ggcctacaat gtacagaatc gtaggtcgga
    38521 taaggtgttc acaccgcatc cgacaacagg tacaaacggc acgataaaaa aatggcactg
    38581 aaggttaaat acccgactaa atcagtcaag taaatagttg accaatttac tcgggaatgt
    38641 cagacttgac cctgctatgc aataccccca cttttacaat aaaaaacccc gggcaggggc
    38701 gagtttgagg tgaagtaaga catgagactg acatctaaag ggcgctatgc cgtgaccgca
    38761 atgcttgacg ttgcgctcaa ctctgaagcg ggcccggtac cgttggctga tatttccgaa
    38821 cgtcagggaa tttccctttc ttatctggaa caactgtttt cccgtctgcg taaaaatggt
    38881 ctggtttcca gcgtacgtgg accaggcggt ggttatctgt taggcaaaga tgccagcagc
    38941 atcgccgttg gcgaagtgat tagcgccgtt gacgaatctg tagatgccac ccgttgtcag
    39001 ggtaaaggcg gctgccaggg cggcgataaa tgcctgaccc acgcgctgtg gcgtgatttg
    39061 agcgaccgtc tcaccggttt tctcaacaac attactttag gcgaactggt taataaccag
    39121 gaagtgctgg atgtgtctgg tcgtcagcat actcacgacg cgccacgcac ccgcacacaa
    39181 gacgcgatcg acgttaagtt acgcgcttaa taaaaagaat tcagaatcag gccggagtgc
    39241 taaatactcc gtaaacacgg tcgtacatcc agccggtagc ctgattcctt gcattgagtg
    39301 atgtacggag tttatagagc aatgaaatta ccgatttatc tcgactactc cgcaaccacg
    39361 ccggtggacc cgcgtgttgc cgagaaaatg atgcagttca tgacgatgga cggaaccttt
    39421 ggtaacccgg cctctcgttc tcaccgtttc ggctggcagg ctgaagaagc ggtagatatc
    39481 gcccgtaatc agattgccga tctggtcggc gctgatccgc gtgaaatcgt ctttacctct
    39541 ggtgcaaccg aatctgacaa cctggcgatc aaaggtgcag ccaactttta tcagaaaaaa
    39601 ggcaagcaca tcatcaccag caaaaccgaa cacaaagcgg tactggatac ttgccgtcag
    39661 ctggagcgcg aaggtttcga agtcacctac ctggcaccgc agcgtaacgg tattatcgac
    39721 ctgaaagaac tcgaagcggc gatgcgtgac gacaccatcc ttgtttccat catgcacgta
    39781 aataacgaaa tcggcgtggt gcaggatatc gcggctatcg gcgaaatgtg ccgtgctcgt
    39841 ggcattatct atcacgttga tgcaacccag agcgtgggca aactgccaat cgacctgagc
    39901 cagttgaaag ttgacctgat gtctttctcc ggtcacaaaa tctatggccc gaaaggtatc
    39961 ggtgcgctgt atgtgcgtcg taaaccgcgt gtacgcatcg aagcgcaaat gcacggcggc
    40021 ggtcacgagc gcggtatgcg ttccggcact ctgcctgttc accagatcgt cggaatgggc
    40081 gaagcctatc gcatcgcaaa agaagagatg gcgaccgaga tggaacgtct gcgcggcctg
    40141 cgtaaccgtc tgtggaacgg catcaaagat atcgaagaag tttacctgaa cggtgacctg
    40201 gaacacggtg cgccgaacat tctcaacgtc agcttcaact acgttgaagg tgagtcgctg
    40261 attatggcgc tgaaagacct cgcagtttct tcaggttccg cctgtacgtc agcaagcctc
    40321 gaaccgtcct acgtgctgcg cgcgctgggg ctgaacgacg agctggcaca tagctctatc
    40381 cgtttctctt taggtcgttt tactactgaa gaagagatcg actacaccat cgagttagtt
    40441 cgtaaatcca tcggtcgtct gcgtgacctt tctccgctgt gggaaatgta caagcagggc
    40501 gtggatctga acagcatcga atgggctcat cattaatcgg tatcggaatc aggagaattt
    40561 ataatggctt acagcgaaaa agttatcgac cattacgaga atccgcgtaa cgtgggttcc
    40621 tttgataaca acgacgagaa cgtcggcagc ggcatggtgg gtgcaccggc ctgtggcgac
    40681 gtaatgaagt tgcagattaa agtcaacgat gaaggtatca ttgaagacgc gcgttttaaa
    40741 acttacggct gcggttccgc tatcgcttcc agctccctgg tcaccgaatg ggtgaaaggg
    40801 aagtctctcg acgaagcgca ggcgatcaaa aacaccgata ttgctgaaga acttgaactg
    40861 ccgccggtga aaattcactg ttctattctg gcagaagacg cgatcaaagc cgccattgcg
    40921 gactataaaa gcaaacgtga agcaaaataa gagttgaggt ttggttatgt cgattacact
    40981 gagcgacagt gcagcagcgc gagtaaatac ctttctggct aaccgcggca aagggtttgg
    41041 cctgcgtctg ggcgtgagaa cctccggatg ttcaggtatg gcttatgtac tggaatttgt
    41101 tgacgaaccg acgccggaag acatcgtgtt tgaagacaaa ggcgtgaaag tcgtggtcga
    41161 tggcaaaagc ctgcaatttc tggacggtac gcagctggac ttcgtaaaag aaggcctgaa
    41221 tgaagggttt aaattcacca acccgaacgt taaagatgag tgtggttgcg gcgaaagctt
    41281 ccacgtttga tgcgcatacc gataacccca ccgtggtcgc ctgcgcgtgg ggtttgtttt
    41341 acctgattcg ccgttatcgc ggcggatcgc agccctgaga atgttatgga ttacttcacc
    41401 ctctttggct tgcctgcccg ttatcaactc gatacccagg cactgagcct gcgttttcag
    41461 gatctacaac gtcagtatca tcctgataaa ttcgccagcg gaagcctggc ggaacaactc
    41521 gccgccgtac agcaatctgc aaccattaac caggcctggc aaacgctgcg tcatccgtta
    41581 atgcgcgcgg aatatttgct ttctttacac ggttttgatc tcgccagcga gcagcatact
    41641 gtgcgcgaca ccgcgttcct gatggaacag ttggagctgc gcgaagagct ggacgagatc
    41701 gaacaggcga aagatgaagc gcggctggaa agctttatca aacgtgtgaa aaagatgttt
    41761 gatacccgcc atcagttgat ggttgaacag ttagacaacg aggcgtggga cgcggcggcg
    41821 gatacggtgc gtaagctgcg ttttctcgat aaactgcgga gcagtgccga acaactcgaa
    41881 gaaaaactgc tcgattttta atttctggaa gctaaacatg gccttattac aaattagtga
    41941 acctggtttg agtgctgcgc cgcatcagcg tcgtctggcg gctggtattg acctgggcac
    42001 aaccaactcg ctggtggcga cagtgcgcag cggtcaggcc gaaacgttag ccgaccatga
    42061 aggccgtcac ctgctgccat ctgttgttca ctatcaacag caagggcatt cggtgggtta
    42121 tgacgcgcgt actaacgcag cgcttgacac cgccaacacc attagttctg ttaaacgcct
    42181 gatggggcgc tcgctggctg atatccagca acgctatccg catctgcctt atcaattcca
    42241 ggccagcgaa aacggcctgc cgatgattga aacggcggcg gggctactga acccggtgcg
    42301 cgtttctgcg gacatcctca aagcactggc ggcgcgggca actgaagccc tggcaggcga
    42361 gctggatggt gtagttatca ccgttccggc gtactttgac gatgcccagc gtcagggcac
    42421 caaagacgcg gcgcgtctgg cggggttgca tgttctgcgc ttacttaacg aaccgactgc
    42481 tgcggctatc gcctacgggc tggattccgg tcaggaaggc gtgatcgctg tttatgacct
    42541 cggtggcggg acgtttgata tttccattct gcgcttaagt cgcggcgtgt ttgaagtgct
    42601 ggcaaccggc ggtgattccg cgctcggcgg cgatgatttc gaccatctgc tggcggatta
    42661 cattcgcgag caggcggaca ttcctgatcg tagcgataac cgcgttcagc gtgaactgct
    42721 ggatgccacc attgcagcca aaatcgcgct gagcgatgcg gactccgtga ccgttaacgt
    42781 tgcgggctgg cagggcgaaa tcagccgtga acaattcaat gaactgatcg cgccactggt
    42841 aaaacgaacc ttactggctt gtcgtcgcgc gctgaaagac gcgggtgtag aagctgacga
    42901 agtgctggaa gtggtgatgg tgggcggttc tactcgcgtg ccgctggtgc gtgaacgggt
    42961 aggcgaattt ttcggtcgtc caccgctgac ttccatcgac ccggataaag tcgtcgctat
    43021 tggcgcggcg attcaggcgg atattctggt gggtaacaag ccagacagcg aaatgctgct
    43081 gcttgatgtg atcccactgt cgctgggcct cgaaacgatg ggcggcctgg tggagaaagt
    43141 gattccgcgt aataccacta ttccggtggc ccgcgctcag gatttcacca cctttaaaga
    43201 tggtcagacg gcgatgtcta tccatgtaat gcagggtgag cgcgaactgg tgcaggactg
    43261 ccgctcactg gcgcgttttg cgctgcgtgg tattccggcg ctaccggctg gcggtgcgca
    43321 tattcgcgtg acgttccagg tcgatgccga cggtcttttg agcgtgacgg cgatggagaa
    43381 atccaccggc gttgaggcgt ctattcaggt caaaccgtct tacggtctga ccgatagcga
    43441 aatcgcttcg atgatcaaag actcaatgag ctatgccgag caggacgtaa aagcccgtat
    43501 gctggcagaa caaaaagtag aagcggcgcg tgtgctggaa agtctgcacg gcgcgctggc
    43561 tgctgatgcc gcgctgttaa gcgccgcaga gcgtcaggtc attgacaatg ctgccgctca
    43621 cctgagtgaa gtggcgcagg gcgatgatgt tgacgccatc gaacaagcga ttaaaaacgt
    43681 agacaaacaa acccaggatt tcgccgctcg ccgcatggac cagtcggttc gtcgtgcgct
    43741 gaaaggccat tccgtggacg aggtttaata tgccaaagat tgttattttg cctcatcagg
    43801 acctctgccc tgatggcgct gttctggaag ctaatagcgg tgaaaccatt ctcgacgcag
    43861 cgctgcgtaa cggtattgag attgaacacg cctgtgaaaa atcctgtgct tgcaccacct
    43921 gccactgcat cgttcgtgaa ggttttgact cactgccgga aagctcagag caggaagacg
    43981 acatgctgga caaagcctgg ggactggagc cggaaagccg tttaagctgc caggcgcgcg
    44041 tcaccgacga agatttagtg gtcgaaatcc cgcgttacac tatcaaccat gcgcgtgagc
    44101 attaacagag gttagtatgg gacttaagtg gaccgatagc cgcgaaattg gcgaagcact
    44161 gtacgatgcg tatcccgatc ttgatccgaa aacggttcga ttcaccgata tgcatcagtg
    44221 gatttgcgat ctggaagatt tcgacgacga cccgcaggca tccaacgaga aaatcctcga
    44281 agcgattttg ttagtctggc tggacgaagc cgagtgaatg aacgggctgc ctgggggtag
    44341 cccgttttgc tgttaaacga ataaggataa ctaaaatgac agaagcgatg aagattaccc
    44401 tctctaccca acctgccgac gcgcgctggg gagaaaaagc aacttacagc attaataatg
    44461 acggcattac cctgcatttg aacggggcag acgatctggg gctgatccag cgtgcggccc
    44521 gcaagattga cggtctgggc atcaagcatg ttcagttaag cggtgaaggc tgggatgcgg
    44581 atcgctgctg ggcattctgg caaggttata aaggcccgaa aggcacgcgt aaagtggagt
    44641 ggccggatct ggacgatgcc cagcgccagg aactggataa ccgcctgatg atcatcgact
    44701 gggtgcgtga caccatcaac gcaccggcag aagaattggg accatcgcaa ctggcacagc
    44761 gtgctgttga tctgatcagc aacgtcgcgg gcgatcgtgt gacttatcgg atcaccaaag
    44821 gcgaagatct gcgtgagcaa ggttatatgg ggctgcacac tgtcgggcgc ggttcagaac
    44881 gttctccggt attgctggcg ctggattaca acccaactgg cgataaagaa gcaccggtgt
    44941 acgcgtgcct ggtaggtaaa ggtatcactt ttgactccgg cggctacagc atcaaacaga
    45001 ctgcgtttat ggactcgatg aagtcggaca tgggcggcgc ggcaacggtt accggggcgc
    45061 tggcatttgc cattatgcgc ggactgaaca agcgcgtgaa gctgttcctc tgctgtgcgg
    45121 ataacctgat tagcggcaat gcgttcaagc tgggcgatat catcacctat cgcaacggta
    45181 aaaaagttga agtgatgaac actgatgccg aagggcgtct ggtgcttgcc gatggtctga
    45241 ttgatgccag tgcgcagaaa ccggaaatga tcattgatgc ggcgaccctc accggggcgg
    45301 cgaaaactgc gctgggtaat gattatcacg cgctgttcag ttttgacgat gcgcttgccg
    45361 gtcgtttact ggcgagtgcc tcacaagaga acgaaccatt ctggcgtctg ccgctggcgg
    45421 aattccaccg cagccagctg ccgtctaact ttgccgaact gaacaatacc ggaagcgcgg
    45481 cgtatccggc aggcgcgagc acggcggcgg gcttcctgtc gcactttgtt gagaactatc
    45541 agcaaggctg gctgcatatc gactgctcgg cgacttaccg taaagcgccg gttgaacagt
    45601 ggtctgcggg cgctacggga cttggtgtgc gcacgatagc taatctgtta acggcgtaaa
    45661 ctaatatgcc ggatgcggcg taaacgcctt atccggccta caaattctgc aaattcaaca
    45721 aattgcgaat cccttgtagg cctgataagc gtagcgcatc aggcatttgc gccccgaacg
    45781 ttgaatgaat tgtggttctt atgtccgaaa caaaaaacga acttgaagac ctgctggaaa
    45841 aagcagcaac cgaaccggcg caccgcccgg cctttttccg tactctactg gaatccaccg
    45901 tctgggtgcc tggtacggcg gcgcagggcg aggctgtggt tgaagatagt gcgcttgatt
    45961 tacagcactg ggaaaaagaa gacggcacca gcgtcattcc ttttttcacc tcgttagaag
    46021 cgcttcaaca ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt cgcacattgt
    46081 ttgagatgac ccttggcgaa acgctcttcc ttaatgccaa actgccgacc ggtaaagaat
    46141 ttatgccgcg tgaaatcagt ttgttgattg gtgaagaggg aaatccgctg agcagccagg
    46201 aaatcctgga aggcggtgaa tcgctgatat tatcggaagt cgcagagccg ccagcacaaa
    46261 tgattgattc actcaccacc ttatttaaaa ccattaagcc ggtgaagcgt gcttttattt
    46321 gttcaattaa agagaacgaa gaggcacagc ctaatttact tattggcatt gaagccgatg
    46381 gtgatatcga agaaattatt caggcgacgg gaagtgtagc gaccgataca ttacctggcg
    46441 atgaaccaat cgatatttgt caggtgaaaa aaggggaaaa aggaattagc cactttatta
    46501 ccgaacatat tgcgccattc tatgaacgtc gctggggtgg ttttttgcgt gactttaaac
    46561 aaaaccggat aatctaaaat atgccgccct aaagggcggc attcgggctt acttgctgct
    46621 ctgaaggttg ttaaccacca gtaaattgtc gccggagaga ctccggttat tatttacttg
    46681 ggaaaacagc gcaccagaaa caataagtac gatacagaag caccgtatct tcattgtgcg
    46741 gtccatgcga agaccgcatc acgacagcac cactggcgat attgccgcga tagcaagcgc
    46801 tgagacgcgc ttgctctaac ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc
    46861 gagacggcgg aaagggccgc agtggataat acactaacag aacaataata actctctttt
    46921 gcttgacaaa aagagagtta ctggcgagta ttgtctccgc tgagacgcgc ttgcattact
    46981 acggtaatac aacctgctgg aaaggccgcg aaccggacca gcaacaaata accgcctgat
    47041 tttggcggtt ttttgttttt taaaggaaag taaatttaca tgtttaagta atatctttta
    47101 ccggttagaa tttttaattt tgagcaggat ttgtttatat ttgtctgaat tcggactaat
    47161 aagccaggtt attttattac cgaacacggc ggaaagggcc ggtgtcgata atacgctaaa
    47221 taaacaataa ttactctctt ttgcttgaca aaaagagagt tactggtgag tattgttttg
    47281 ctgtgaagca cctgcgttgc tcatgtgtaa cacaagtcaa cctgctggaa aggccgcgaa
    47341 ccagaccagc aataaaaaaa cgccaaattc ggcggttttt tattgcctgt aaaatactta
    47401 tttcactggc tcaaccggta aatctgcccg cgcgccccat tcactccatg cgccgtcgta
    47461 cagtttcacg tttggcacat ccagcgtcgc gagtgctaac aaaaccacgg ctgccgttac
    47521 accagagccg cagctgacga taatcggttt gtcatagctg acgccgcgac caaaaaatat
    47581 cgcatccagt tcatcggtcg tttttagttc gccttcgcgc accagttccg tccacggaac
    47641 gttcaatgca ccgggaatat gtccgcgacg taaacctggg cgaggttcat caacttctgc
    47701 gttaaaacgt gcagccgggc gggcatcaat aatttgcgcc gtattttcat ggcttgccaa
    47761 taacacatcg gttactttca ccactgcttc aggattaaac gcggcgttaa actctccttc
    47821 cggcagctct actgcacctt cttctaatag caggtcatcg cgctgccagc ctgcaag
//
LOCUS       DAJTNW010000023        45806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNW010000023 DAJTNW010000000
VERSION     DAJTNW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            114..2267
                     /locus_tag="OO179_001347"
     CDS             114..2267
                     /locus_tag="OO179_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5531474.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO179_001348"
     CDS             2320..4050
                     /locus_tag="OO179_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531475.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO179_001349"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO179_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5531476.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO179_001350"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO179_001350"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5531477.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO179_001351"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO179_001351"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5531478.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO179_001352"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO179_001352"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5531479.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO179_001353"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO179_001353"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5531480.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO179_001354"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO179_001354"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5531481.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO179_001355"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO179_001355"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5531482.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO179_001356"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO179_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5531483.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO179_001357"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO179_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5531484.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO179_001358"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO179_001358"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5531485.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO179_001359"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO179_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5531486.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO179_001360"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO179_001360"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5531487.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO179_001361"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO179_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5531488.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO179_001362"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO179_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5531489.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO179_001363"
     CDS             16308..16847
                     /locus_tag="OO179_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5531490.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO179_001364"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO179_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5531491.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO179_001365"
     CDS             complement(18206..18553)
                     /locus_tag="OO179_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5531492.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO179_001366"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO179_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5531493.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO179_001367"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO179_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5531494.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO179_001368"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO179_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5531495.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO179_001369"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO179_001369"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5531496.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO179_001370"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO179_001370"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5531497.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO179_001371"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO179_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO179_001372"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO179_001372"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5531498.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO179_001373"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO179_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5531499.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO179_001374"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO179_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5531500.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO179_001375"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO179_001375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5531501.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO179_001376"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO179_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO179_001377"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO179_001377"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5531502.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO179_001378"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO179_001378"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5531503.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO179_001379"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO179_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5531504.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO179_001380"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO179_001380"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5531505.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO179_001381"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO179_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5531506.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO179_001382"
     CDS             complement(40011..40466)
                     /locus_tag="OO179_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531507.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO179_001383"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO179_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO179_001384"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO179_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5531508.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO179_001385"
     CDS             complement(41309..41986)
                     /locus_tag="OO179_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5531509.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO179_001386"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO179_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5531510.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO179_001387"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO179_001387"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5531511.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO179_001388"
     CDS             complement(43192..43650)
                     /locus_tag="OO179_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5531512.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO179_001389"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO179_001389"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5531513.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO179_001390"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO179_001390"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5531514.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11910 a        11943 c        11204 g        10749 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct cacccgttag cactat
//
LOCUS       DAJTNW010000024        43995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNW010000024 DAJTNW010000000
VERSION     DAJTNW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(15..602)
                     /gene="mog"
                     /locus_tag="OO179_001391"
     CDS             complement(15..602)
                     /gene="mog"
                     /locus_tag="OO179_001391"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5531515.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(717..1670)
                     /gene="tal"
                     /locus_tag="OO179_001392"
     CDS             complement(717..1670)
                     /gene="tal"
                     /locus_tag="OO179_001392"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5531516.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1949..3378
                     /locus_tag="OO179_001393"
                     /pseudo
     CDS             1949..3378
                     /locus_tag="OO179_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3448..4224
                     /gene="yaaA"
                     /locus_tag="OO179_001394"
     CDS             3448..4224
                     /gene="yaaA"
                     /locus_tag="OO179_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5531517.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4377..4673)
                     /locus_tag="OO179_001395"
     CDS             complement(4377..4673)
                     /locus_tag="OO179_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5531518.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="OO179_001396"
     CDS             complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="OO179_001396"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5531519.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="OO179_001397"
     CDS             complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="OO179_001397"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5531520.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="OO179_001398"
     CDS             complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="OO179_001398"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5531521.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9596..9715)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="OO179_001399"
     CDS             complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="OO179_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5531522.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9930..10616)
                     /locus_tag="OO179_001400"
     CDS             complement(9930..10616)
                     /locus_tag="OO179_001400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5531523.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11016..11156)
                     /locus_tag="OO179_001401"
     CDS             complement(11016..11156)
                     /locus_tag="OO179_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531524.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11252..11968
                     /gene="arcA"
                     /locus_tag="OO179_001402"
     CDS             11252..11968
                     /gene="arcA"
                     /locus_tag="OO179_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5531525.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12027..13379)
                     /gene="creD"
                     /locus_tag="OO179_001403"
     CDS             complement(12027..13379)
                     /gene="creD"
                     /locus_tag="OO179_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5531526.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13437..14861)
                     /gene="creC"
                     /locus_tag="OO179_001404"
     CDS             complement(13437..14861)
                     /gene="creC"
                     /locus_tag="OO179_001404"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5531527.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14861..15550)
                     /gene="creB"
                     /locus_tag="OO179_001405"
     CDS             complement(14861..15550)
                     /gene="creB"
                     /locus_tag="OO179_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5531528.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15563..16036)
                     /gene="creA"
                     /locus_tag="OO179_001406"
     CDS             complement(15563..16036)
                     /gene="creA"
                     /locus_tag="OO179_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5531529.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16247..17116
                     /gene="robA"
                     /locus_tag="OO179_001407"
     CDS             16247..17116
                     /gene="robA"
                     /locus_tag="OO179_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5531530.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="OO179_001408"
     CDS             complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="OO179_001408"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5531531.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17812..18332
                     /gene="yjjX"
                     /locus_tag="OO179_001409"
                     /pseudo
     CDS             17812..18332
                     /gene="yjjX"
                     /locus_tag="OO179_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="OO179_001410"
     CDS             complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="OO179_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5531532.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="OO179_001411"
     CDS             complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="OO179_001411"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5531533.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20981..22648
                     /gene="ettA"
                     /locus_tag="OO179_001412"
     CDS             20981..22648
                     /gene="ettA"
                     /locus_tag="OO179_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5531534.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22755..22922)
                     /locus_tag="OO179_001413"
     CDS             complement(22755..22922)
                     /locus_tag="OO179_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531535.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="OO179_001414"
     CDS             complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="OO179_001414"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5531536.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24209..25591)
                     /gene="radA"
                     /locus_tag="OO179_001415"
     CDS             complement(24209..25591)
                     /gene="radA"
                     /locus_tag="OO179_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5531537.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25640..26608)
                     /gene="serB"
                     /locus_tag="OO179_001416"
     CDS             complement(25640..26608)
                     /gene="serB"
                     /locus_tag="OO179_001416"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5531538.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26714..27358
                     /locus_tag="OO179_001417"
     CDS             26714..27358
                     /locus_tag="OO179_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5531539.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27386..28401
                     /gene="lplA"
                     /locus_tag="OO179_001418"
                     /pseudo
     CDS             27386..28401
                     /gene="lplA"
                     /locus_tag="OO179_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28433..28696
                     /locus_tag="OO179_001419"
     CDS             28433..28696
                     /locus_tag="OO179_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5531540.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="OO179_001420"
     CDS             complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="OO179_001420"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5531541.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="OO179_001421"
     CDS             complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="OO179_001421"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5531542.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="OO179_001422"
     CDS             complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="OO179_001422"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5531543.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="OO179_001423"
     CDS             complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="OO179_001423"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5531544.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33394..34935
                     /locus_tag="OO179_001424"
     CDS             33394..34935
                     /locus_tag="OO179_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5531545.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34907..35770
                     /locus_tag="OO179_001425"
     CDS             34907..35770
                     /locus_tag="OO179_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5531546.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35984..36766)
                     /locus_tag="OO179_001426"
     CDS             complement(35984..36766)
                     /locus_tag="OO179_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5531547.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36763..37836)
                     /locus_tag="OO179_001427"
     CDS             complement(36763..37836)
                     /locus_tag="OO179_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5531548.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37958..38119)
                     /locus_tag="OO179_001428"
     CDS             complement(37958..38119)
                     /locus_tag="OO179_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5531549.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="OO179_001429"
     CDS             complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="OO179_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5531550.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="OO179_001430"
     CDS             complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="OO179_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5531551.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="OO179_001431"
     CDS             complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="OO179_001431"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5531552.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="OO179_001432"
     CDS             complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="OO179_001432"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5531553.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42021..42434)
                     /gene="holD"
                     /locus_tag="OO179_001433"
     CDS             complement(42021..42434)
                     /gene="holD"
                     /locus_tag="OO179_001433"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5531554.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42537..43568
                     /gene="rsmC"
                     /locus_tag="OO179_001434"
     CDS             42537..43568
                     /gene="rsmC"
                     /locus_tag="OO179_001434"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5531555.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43845..43931
                     /locus_tag="OO179_001435"
     tRNA            43845..43931
                     /locus_tag="OO179_001435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43879..43881,aa:Leu,seq:cag)
BASE COUNT        10354 a        12026 c        11142 g        10473 t
ORIGIN      
        1 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
       61 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
      121 ctggcaaaaa taccatgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
      181 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg
      241 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
      301 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
      361 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
      421 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
      481 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgctggaat gcctttatcc
      541 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
      601 atcaagattt ttccgtttaa acaatctcgt taaacagaat gatacactgc gaagggagtg
      661 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
      721 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
      781 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
      841 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
      901 ccttcgctct ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcacagcct
      961 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
     1021 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
     1081 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
     1141 ggcgagatca ggaacacgcc cgcctccgca caagcacgag cctgagcgaa ggagaacagc
     1201 agggtcaggt tacagttgat accttctttt tccagctgtt ctgcagcacg gataccctgc
     1261 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
     1321 ttgatcaggc gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca
     1381 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
     1441 agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
     1501 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
     1561 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
     1621 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
     1681 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
     1741 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
     1801 actgcgttga tatcgcccac catttttata caaaacctca tgtatgctac gcagaagtta
     1861 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
     1921 atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
     1981 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
     2041 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
     2101 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
     2161 cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gaccaggtgc
     2221 cgtcttttgg atgtgggttg ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc
     2281 ccttgcgcaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
     2341 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
     2401 catcgcctac ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag
     2461 tttttctttt gattttcctc cgctggtgac gggcattatt ctcgctgtct ttgctctgct
     2521 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     2581 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
     2641 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     2701 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     2761 tgaggtggga atgggatcga cgccaaacgc ggcagcggca gctgcatcct ggcctccgca
     2821 tccggcagca caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     2881 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
     2941 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt
     3001 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     3061 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     3121 ctgcaccttc gcaacgatca tcggcggcac cttgctaagc cttccgctga tgtggcaact
     3181 ggcagatata taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
     3241 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     3301 gtgtttgatc cgttgcgtta tccggatatc ggtcgccaac tttctccgga cgcgtgggat
     3361 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
     3421 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
     3481 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
     3541 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
     3601 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
     3661 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
     3721 caggccgaga ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     3781 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     3841 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     3901 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     3961 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     4021 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
     4081 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
     4141 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
     4201 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
     4261 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
     4321 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
     4381 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
     4441 cgcggcggtg acggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
     4501 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
     4561 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
     4621 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
     4681 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
     4741 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
     4801 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
     4861 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
     4921 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
     4981 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
     5041 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
     5101 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
     5161 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
     5221 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
     5281 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
     5341 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
     5401 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
     5461 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
     5521 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
     5581 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
     5641 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
     5701 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
     5761 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
     5821 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
     5881 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
     5941 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
     6001 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
     6061 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
     6121 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
     6181 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
     6241 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
     6301 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
     6361 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
     6421 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
     6481 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
     6541 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
     6601 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
     6661 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
     6721 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
     6781 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
     6841 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
     6901 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
     6961 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
     7021 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
     7081 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
     7141 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
     7201 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
     7261 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
     7321 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
     7381 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
     7441 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
     7501 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
     7561 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
     7621 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
     7681 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
     7741 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
     7801 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtaacaa cgtggaaacc
     7861 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
     7921 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
     7981 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
     8041 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
     8101 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
     8161 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
     8221 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
     8281 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
     8341 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
     8401 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
     8461 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
     8521 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
     8581 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
     8641 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
     8701 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
     8761 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
     8821 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
     8881 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
     8941 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
     9001 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
     9061 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
     9121 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
     9181 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
     9241 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
     9301 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
     9361 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
     9421 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
     9481 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
     9541 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
     9601 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     9661 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
     9721 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     9781 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     9841 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
     9901 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     9961 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    10021 cagtcgacca gttttatgtc atctgccact gccagagccg tcagcaatgc catgactcgt
    10081 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    10141 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    10201 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    10261 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    10321 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    10381 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    10441 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    10501 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    10561 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    10621 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    10681 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    10741 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    10801 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    10861 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    10921 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    10981 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    11041 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    11101 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    11161 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    11221 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    11281 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    11341 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    11401 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    11461 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    11521 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    11581 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    11641 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    11701 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    11761 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    11821 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    11881 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    11941 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    12001 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    12061 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    12121 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    12181 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gaggataaac
    12241 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    12301 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    12361 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    12421 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    12481 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    12541 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    12601 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    12661 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    12721 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    12781 gccatattca gctgcaggtt ctgcttacgc cagtcccctt cgggtaaagg gatatgcacg
    12841 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    12901 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    12961 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    13021 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    13081 acgttctgat tgccatcaac catcaatgac tcaggtaaca aaaaatggat aaaacttcgt
    13141 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    13201 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    13261 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    13321 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    13381 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    13441 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    13501 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    13561 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    13621 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    13681 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    13741 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    13801 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    13861 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    13921 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    13981 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    14041 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    14101 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    14161 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    14221 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    14281 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    14341 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    14401 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    14461 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    14521 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    14581 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    14641 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    14701 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    14761 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    14821 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    14881 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    14941 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    15001 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    15061 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    15121 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    15181 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    15241 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    15301 gggccgtcag gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt
    15361 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    15421 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    15481 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    15541 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    15601 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    15661 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    15721 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    15781 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    15841 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    15901 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    15961 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    16021 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    16081 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    16141 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    16201 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    16261 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    16321 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    16381 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    16441 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    16501 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    16561 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    16621 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    16681 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    16741 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    16801 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    16861 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    16921 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    16981 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    17041 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    17101 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    17161 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    17221 cgaaatagaa cagttacgca gacgtaagcg gcgctctacc catgctggta atccgagaat
    17281 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    17341 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    17401 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    17461 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    17521 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    17581 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    17641 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    17701 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    17761 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    17821 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    17881 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    17941 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    18001 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    18061 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    18121 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    18181 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    18241 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    18301 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    18361 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    18421 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    18481 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    18541 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    18601 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    18661 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    18721 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    18781 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    18841 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    18901 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    18961 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    19021 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    19081 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    19141 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    19201 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    19261 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    19321 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    19381 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    19441 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    19501 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    19561 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    19621 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    19681 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    19741 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    19801 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    19861 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    19921 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    19981 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    20041 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    20101 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    20161 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    20221 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    20281 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    20341 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    20401 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    20461 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    20521 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    20581 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    20641 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    20701 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    20761 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    20821 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    20881 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    20941 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    21001 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    21061 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    21121 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    21181 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    21241 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    21301 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    21361 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    21421 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    21481 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    21541 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    21601 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    21661 ctggatcctc gaactggacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg
    21721 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    21781 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    21841 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    21901 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    21961 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    22021 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    22081 tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa
    22141 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    22201 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    22261 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    22321 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    22381 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    22441 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    22501 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    22561 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    22621 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    22681 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    22741 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    22801 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    22861 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    22921 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    22981 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    23041 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    23101 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    23161 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    23221 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    23281 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag
    23341 tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    23401 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    23461 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    23521 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    23581 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    23641 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    23701 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    23761 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    23821 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    23881 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    23941 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    24001 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    24061 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    24121 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    24181 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    24241 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    24301 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    24361 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    24421 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcgccgctgg
    24481 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    24541 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    24601 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    24661 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    24721 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    24781 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    24841 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    24901 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    24961 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    25021 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    25081 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    25141 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    25201 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    25261 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    25321 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    25381 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    25441 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    25501 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    25561 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    25621 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    25681 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    25741 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    25801 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    25861 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    25921 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    25981 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    26041 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    26101 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    26161 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    26221 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    26281 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    26341 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    26401 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    26461 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    26521 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    26581 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    26641 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    26701 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    26761 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    26821 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    26881 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    26941 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    27001 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    27061 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    27121 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    27181 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    27241 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    27301 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    27361 cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta
    27421 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    27481 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    27541 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    27601 tggcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
    27661 caagccggag tacgataaaa ctctctccac gtcgattgtg ctcaatgcgc tgaacgcgct
    27721 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
    27781 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
    27841 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
    27901 actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct
    27961 gccggggctc accatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    28021 ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    28081 gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc tccggcattc
    28141 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    28201 aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaacctcgc gccgctggaa
    28261 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    28321 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    28381 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    28441 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    28501 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    28561 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    28621 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    28681 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtttttta ttttctatcc
    28741 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    28801 ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac
    28861 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc
    28921 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg
    28981 gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc ttccatttcc
    29041 acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    29101 aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt agctgcatct
    29161 actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc tttaaaacgg
    29221 atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc gcgcagtttt
    29281 acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac gccgaaatcg
    29341 gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg
    29401 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    29461 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    29521 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    29581 ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc catcagaaca
    29641 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    29701 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca tataccagta
    29761 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa ccgtggtcag
    29821 cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    29881 cgaacagttc cagacccgca gcataaccgg cgacgtcgcg gcggtggccc caggaagagt
    29941 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    30001 tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg ataccgcagt
    30061 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt tcatcaacca
    30121 gtttctgcag cacggtcggt gctggcggct caacagccaa gtcgtgacgg ttaccggtac
    30181 gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga ccgatattgt
    30241 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac
    30301 cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta tagaaaatcg
    30361 gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt
    30421 ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc agcagctctt
    30481 gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg
    30541 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    30601 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    30661 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggc cctttacgac
    30721 cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    30781 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    30841 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    30901 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gcgttatcgg
    30961 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    31021 cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag
    31081 taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    31141 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    31201 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    31261 tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    31321 gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagatca
    31381 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    31441 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    31501 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    31561 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    31621 tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg atcgaggcag
    31681 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    31741 cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct ttaataattt
    31801 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    31861 gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg
    31921 ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt
    31981 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    32041 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    32101 tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg ttgataaaga
    32161 aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg
    32221 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    32281 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    32341 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg cccagcgctt
    32401 tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tcagcccagt
    32461 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    32521 cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    32581 tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    32641 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    32701 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    32761 tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc gcgcggtacg
    32821 ggaacaccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    32881 cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata cggatttccg
    32941 gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga tagatacaga
    33001 tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt actttctcgt
    33061 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    33121 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    33181 tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa ttaaggaaat
    33241 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    33301 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    33361 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    33421 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    33481 tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc cgaagcccgc
    33541 cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    33601 cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg gctggagctg
    33661 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    33721 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat
    33781 gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    33841 gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    33901 acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt tatctacgat
    33961 cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc
    34021 aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    34081 attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    34141 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    34201 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    34261 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    34321 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgagaaa
    34381 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc
    34441 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    34501 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    34561 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    34621 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac
    34681 ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    34741 gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    34801 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    34861 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    34921 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    34981 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    35041 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    35101 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagat
    35161 gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag tgcttagcca
    35221 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    35281 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    35341 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    35401 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    35461 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    35521 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    35581 cgaagaactg gcggcgttta tcaagggact tggtgatgtt ccggttcgcc tgaacgcgtt
    35641 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    35701 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    35761 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    35821 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    35881 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
    35941 ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca cgaacgttaa
    36001 acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt tccgggcgta
    36061 actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc tgcccctgaa
    36121 aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc agcggtaatt
    36181 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
    36241 ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa aatccgtgga
    36301 caacaccagc acgcggcaaa tcgtggtgtt taagatgcat cgccagtttg tcgtgcgtgc
    36361 gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt tgttcgtcga
    36421 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
    36481 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc tcaagagaca
    36541 catcgctatg tttttccaac ataccaggat gcaagcccaa tgcggcatac agcggttgat
    36601 aattttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc ggaacaatga
    36661 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccaataaaag
    36721 gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca gatcctcatt
    36781 gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca gcgtatcgtt
    36841 ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca ccagatggcg
    36901 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
    36961 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
    37021 atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg gtttctcaat
    37081 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
    37141 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
    37201 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
    37261 aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca gcgacactcc
    37321 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
    37381 ggttggtaaa aagtaattcg gctcgtagtc atcctgacga caggcgcaca tataaaacga
    37441 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
    37501 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
    37561 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
    37621 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
    37681 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
    37741 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
    37801 aatattacca agcgttacag gtattcgctg ccccacttct gccttcctgt ttttgttgtg
    37861 aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
    37921 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
    37981 acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
    38041 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
    38101 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
    38161 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
    38221 gttcgggagg acgaattact tagttttcag atcattttta acgcttttca caccatctac
    38281 cgctttggcg atactttcag cacggtcact ttgtgcctga gaatctacgg taccggagag
    38341 ctgaaccacg ccatcggtgg tttcaacttt cactttacgt gaagggacga tatcgtccgc
    38401 cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc ccttcaccga
    38461 gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc
    38521 tttcgccact ttcactgcct cttcggcctg ggcctgactt tcaacgaaac cgctcagggt
    38581 cacgactttt tgatcggttt tgacagagat atcggtgctc ttgatgttgt catgatccac
    38641 cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac cgactttatt
    38701 catagagcta tcgacttttt gccctgcgct ttcattggta gtctgcgcgt tgttttccgc
    38761 gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag ttttcgaaat
    38821 cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag
    38881 caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa gtcaatggtc
    38941 gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa tcagttatta
    39001 atatcggtag aatgacaatc gacggcttta gatactgata tctacgcatt gaacggtatt
    39061 taacgccatc agaaatgtca tcactttgtt aaatatagat cacaattttg aaaccgctcg
    39121 ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt aagcggctaa
    39181 taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc
    39241 tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg caggttgacc
    39301 atgctggtag cgatgtaagc gaggttatcg ccgccatcaa gcgccagttg gctttcgttc
    39361 ttacgcttga actcttcgaa tttcttcgcg tctgcacatt ctacccagcg ggcagtggca
    39421 acgttgactg actcatacac tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc
    39481 acatcaaact gcagcacacc aaccgcacca acgatcagat cgttgttgga gattggacgg
    39541 aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc
    39601 ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt cggaataccg
    39661 gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg
    39721 tgcaggccga ggatatcgcc cggatacgct tcttcaacgt gcgaacggtc acccgccata
    39781 aaggtcagcg cgtcggagat caccacatct ttcgcagtgc gcacctggcg cagtttcatg
    39841 cctttttcat atttaccgga caccacacgc ataaacgcca cgcggtcgcg gtgtttcgga
    39901 tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tatcttcgct cgcttctacg
    39961 gtacgggtat cggtctgacg cggcatcggc gcaggcgccc actccaccag gccattcagc
    40021 atatgatcaa cgccgaagtt acccagcgca gtaccgaaga ataccggagt gatttcgccc
    40081 gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc cagttcgtca
    40141 cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt attcagccct
    40201 ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag ataggtttca
    40261 tctttataaa ggtggtaaac gcctttaaac agcttgccgc agccaattgg ccaagtgatc
    40321 ggtgcgcagc caattttcag ctcgttctca acttcatcga gcagttccat cgggtcgcgg
    40381 atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag acgggtaact
    40441 tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac catcaggcag
    40501 cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcgtg ccccggggtg
    40561 tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt agtaatggag
    40621 atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt ggaaccacgg
    40681 ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg
    40741 gtagttttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt cgccacctct
    40801 ttcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat cgcgccgcgc
    40861 ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg caatcagtgt
    40921 ttacacagga gctgctccag ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct
    40981 tctggttgct cgcgattgtg cgcattcagc cagcaggtcg caagcccggc gttgatgcca
    41041 ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg gtcaggattg
    41101 cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttggcaac gccaacttct
    41161 tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag acgcacttgc
    41221 tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgctacg aatggcgtta
    41281 agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt aataaaggca
    41341 tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcgaa acgcccgtgc
    41401 tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg cttgttaacg
    41461 gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc
    41521 tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag
    41581 tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg atgatggcgt
    41641 cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta
    41701 aactttcgta cagggcaatg gcggcagcat ttgaggcacg gacttccagc catagtgtcg
    41761 ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcgcccta cccagcccct
    41821 gacgctgata gtcgggatcg accgcaatat tgaacaatgt agcttcatcc agcaccactt
    41881 gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg agataacgct
    41941 cgccctggtt gctggcaaag gttttttcac tccacggaaa ggagtgggcg cgttgttcaa
    42001 tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa
    42061 atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt
    42121 gatgccacct gagcgccttc caatgatagc ggttcgtcag tacccaaccg ccagctgttg
    42181 cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag cacttgatcg
    42241 gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcagtcag ggcgggaaga
    42301 tcgtttgcca ccatcaccag acggacgtgt gccgggctcg caatggcaat ctccccctgc
    42361 aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa ctgccagtct
    42421 cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc
    42481 gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag cattccatgt
    42541 ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc
    42601 gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat accgcggcca
    42661 gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt aagctgccag atgggggata
    42721 acgcccgttt tagtctggtc gccacggcga atgatgtcgc agattgcgat acgctgattt
    42781 actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta ctttctctgc
    42841 tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg
    42901 agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc
    42961 tctattttgg tcgtctggaa aaacagccag tatttgatgc ggataaattc tggggcgaat
    43021 acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg
    43081 atgtcggtag ccagttgctg ctctcgacgt taactccgca cacgaaaggt aaagtgctgg
    43141 atgtcggctg tggcgcgggt gtactttcag ttgcctttgc gcgccattcg ccgaaaattc
    43201 gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc cagccgcgca acacttgcag
    43261 ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt
    43321 ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg
    43381 cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg
    43441 tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc ttccatgaag
    43501 ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat tatgacccgc caggcgaaga
    43561 aaggttgagt atctgatatc tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta
    43621 tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc gccagcgtcg
    43681 catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct gatggacatt
    43741 tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga aaaccactag
    43801 aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat
    43861 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    43921 ccctcgcacc aaaaaccacg ttgatattgc tcgcactggg cgaaggtggc ggaattggta
    43981 gacgcgctag cttca
//
LOCUS       DAJTNW010000025        40804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNW010000025 DAJTNW010000000
VERSION     DAJTNW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="OO179_001436"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="OO179_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5531556.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO179_001437"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO179_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5531557.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO179_001438"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO179_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5531558.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="OO179_001439"
     CDS             complement(3100..6843)
                     /locus_tag="OO179_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5531559.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO179_001440"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO179_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5531560.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="OO179_001441"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="OO179_001441"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5531561.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="OO179_001442"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="OO179_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5531562.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO179_001443"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO179_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5531563.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="OO179_001444"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="OO179_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5531564.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO179_001445"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO179_001445"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5531565.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO179_001446"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO179_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5531566.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="OO179_001447"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="OO179_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5531567.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="OO179_001448"
     CDS             16240..16347
                     /locus_tag="OO179_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5531568.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO179_001449"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO179_001449"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5531569.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="OO179_001450"
     CDS             complement(17386..18195)
                     /locus_tag="OO179_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5531570.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="OO179_001451"
     CDS             complement(18199..18591)
                     /locus_tag="OO179_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5531571.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO179_001452"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO179_001452"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5531572.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO179_001453"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO179_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5531573.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO179_001454"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO179_001454"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5531574.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="OO179_001455"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="OO179_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5531575.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="OO179_001456"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="OO179_001456"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5531576.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="OO179_001457"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="OO179_001457"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5531577.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="OO179_001458"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="OO179_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5531578.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO179_001459"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO179_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5531579.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="OO179_001460"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="OO179_001460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5531580.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="OO179_001461"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="OO179_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5531581.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="OO179_001462"
     CDS             29637..32504
                     /locus_tag="OO179_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5531582.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO179_001463"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO179_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5531583.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="OO179_001464"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="OO179_001464"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5531584.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="OO179_001465"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="OO179_001465"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5531585.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="OO179_001466"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="OO179_001466"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5531586.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="OO179_001467"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="OO179_001467"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5531587.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="OO179_001468"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="OO179_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10087 a        10575 c        10177 g         9965 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct atgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 atgg
//
LOCUS       DAJTNW010000026        39797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNW010000026 DAJTNW010000000
VERSION     DAJTNW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..145
                     /locus_tag="OO179_001469"
     CDS             <1..145
                     /locus_tag="OO179_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5531588.1"
                     /translation="MLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQ
                     ELN"
     gene            374..964
                     /gene="uidR"
                     /locus_tag="OO179_001470"
     CDS             374..964
                     /gene="uidR"
                     /locus_tag="OO179_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5531589.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1352..3025
                     /gene="uidA"
                     /locus_tag="OO179_001471"
     CDS             1352..3025
                     /gene="uidA"
                     /locus_tag="OO179_001471"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5531590.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3061..4086
                     /gene="uidB"
                     /locus_tag="OO179_001472"
                     /pseudo
     CDS             3061..4086
                     /gene="uidB"
                     /locus_tag="OO179_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4125..5390
                     /gene="uidC"
                     /locus_tag="OO179_001473"
     CDS             4125..5390
                     /gene="uidC"
                     /locus_tag="OO179_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5531591.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5616..7124)
                     /locus_tag="OO179_001474"
     CDS             complement(5616..7124)
                     /locus_tag="OO179_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5531592.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7225..8400)
                     /gene="manA"
                     /locus_tag="OO179_001475"
     CDS             complement(7225..8400)
                     /gene="manA"
                     /locus_tag="OO179_001475"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5531593.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8599..10245
                     /gene="fumB"
                     /locus_tag="OO179_001476"
     CDS             8599..10245
                     /gene="fumB"
                     /locus_tag="OO179_001476"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5531594.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10388..11791
                     /gene="fumC"
                     /locus_tag="OO179_001477"
     CDS             10388..11791
                     /gene="fumC"
                     /locus_tag="OO179_001477"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5531595.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11788..12717)
                     /gene="tus"
                     /locus_tag="OO179_001478"
     CDS             complement(11788..12717)
                     /gene="tus"
                     /locus_tag="OO179_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5531596.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12793..14094)
                     /gene="rstB"
                     /locus_tag="OO179_001479"
     CDS             complement(12793..14094)
                     /gene="rstB"
                     /locus_tag="OO179_001479"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5531597.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14273..15592)
                     /locus_tag="OO179_001480"
     CDS             complement(14273..15592)
                     /locus_tag="OO179_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5531598.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15817..16560)
                     /gene="rstA"
                     /locus_tag="OO179_001481"
     CDS             complement(15817..16560)
                     /gene="rstA"
                     /locus_tag="OO179_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5531599.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16689..17024
                     /locus_tag="OO179_001482"
     CDS             16689..17024
                     /locus_tag="OO179_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5531600.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17021..17743)
                     /gene="folM"
                     /locus_tag="OO179_001483"
     CDS             complement(17021..17743)
                     /gene="folM"
                     /locus_tag="OO179_001483"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5531601.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17780..19162)
                     /locus_tag="OO179_001484"
     CDS             complement(17780..19162)
                     /locus_tag="OO179_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5531602.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAILILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19348..20292)
                     /locus_tag="OO179_001485"
     CDS             complement(19348..20292)
                     /locus_tag="OO179_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5531603.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20816..22348
                     /gene="pntA"
                     /locus_tag="OO179_001486"
     CDS             20816..22348
                     /gene="pntA"
                     /locus_tag="OO179_001486"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5531604.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22359..23747
                     /gene="pntB"
                     /locus_tag="OO179_001487"
     CDS             22359..23747
                     /gene="pntB"
                     /locus_tag="OO179_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5531605.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23772..24806)
                     /gene="tqsA"
                     /locus_tag="OO179_001488"
     CDS             complement(23772..24806)
                     /gene="tqsA"
                     /locus_tag="OO179_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5531606.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25217..25582
                     /gene="mdtJ"
                     /locus_tag="OO179_001489"
     CDS             25217..25582
                     /gene="mdtJ"
                     /locus_tag="OO179_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5531607.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25569..25898
                     /gene="mdtI"
                     /locus_tag="OO179_001490"
     CDS             25569..25898
                     /gene="mdtI"
                     /locus_tag="OO179_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5531608.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25937..26758)
                     /locus_tag="OO179_001491"
     CDS             complement(25937..26758)
                     /locus_tag="OO179_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5531609.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26858..26941)
                     /locus_tag="OO179_001492"
     CDS             complement(26858..26941)
                     /locus_tag="OO179_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531610.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27034..27369)
                     /locus_tag="OO179_001493"
     CDS             complement(27034..27369)
                     /locus_tag="OO179_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5531611.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27937..28194)
                     /locus_tag="OO179_001494"
     CDS             complement(27937..28194)
                     /locus_tag="OO179_001494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531612.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28210..29019)
                     /locus_tag="OO179_001495"
     CDS             complement(28210..29019)
                     /locus_tag="OO179_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5531613.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29126..30028
                     /locus_tag="OO179_001496"
     CDS             29126..30028
                     /locus_tag="OO179_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5531614.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30163..31383
                     /gene="mlc"
                     /locus_tag="OO179_001497"
                     /gene_synonym="dgsA"
     CDS             30163..31383
                     /gene="mlc"
                     /locus_tag="OO179_001497"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5531615.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31508..32203
                     /gene="bioD"
                     /locus_tag="OO179_001498"
     CDS             31508..32203
                     /gene="bioD"
                     /locus_tag="OO179_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5531616.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32156..33448)
                     /gene="clcB"
                     /locus_tag="OO179_001499"
     CDS             complement(32156..33448)
                     /gene="clcB"
                     /locus_tag="OO179_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5531617.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33607..34221)
                     /gene="dmsD"
                     /locus_tag="OO179_001500"
     CDS             complement(33607..34221)
                     /gene="dmsD"
                     /locus_tag="OO179_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5531618.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34264..35118)
                     /locus_tag="OO179_001501"
     CDS             complement(34264..35118)
                     /locus_tag="OO179_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5531619.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35120..35737)
                     /gene="dmsB"
                     /locus_tag="OO179_001502"
     CDS             complement(35120..35737)
                     /gene="dmsB"
                     /locus_tag="OO179_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5531620.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35748..37786)
                     /locus_tag="OO179_001503"
                     /pseudo
     CDS             complement(35748..37786)
                     /locus_tag="OO179_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37847..38956)
                     /locus_tag="OO179_001504"
     CDS             complement(37847..38956)
                     /locus_tag="OO179_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5531621.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38939..39736
                     /locus_tag="OO179_001505"
                     /pseudo
     CDS             38939..39736
                     /locus_tag="OO179_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT        10123 a         9649 c        10190 g         9835 t
ORIGIN      
        1 aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg ctaacgcggc
       61 gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca ccgtctccgg
      121 tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc gtatgtgttg
      181 tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg cagccaaatc
      241 acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt accttcgcac
      301 tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta ttcacgctaa
      361 ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac ggacccggat
      421 cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct cgatgaaagc
      481 catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca tctccaaaga
      541 agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc gtttccggga
      601 accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct ctctcaccca
      661 tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga tgcgtaaccc
      721 acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg ttgcccagcg
      781 gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg caatgacttc
      841 acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg aagacctggc
      901 gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat taaccgcatc
      961 ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat cgtaatttct
     1021 ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat tcaccgtagc
     1081 catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga ttgcttattt
     1141 tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc tgtgggtatt
     1201 gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc tcttccatgg
     1261 gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat taaaggttga
     1321 ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa ccccaacccg
     1381 tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
     1441 tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag tgccaggcag
     1501 ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg tctggtatca
     1561 gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
     1621 ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
     1681 ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
     1741 caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
     1801 cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccgggat
     1861 ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
     1921 gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
     1981 tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
     2041 cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
     2101 tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc tgcgcgtcgg
     2161 catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt
     2221 tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg ataacgtgct
     2281 gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc gtacctcgca
     2341 ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
     2401 tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
     2461 gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg cgcacttaca
     2521 ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
     2581 tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga
     2641 agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga
     2701 cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg
     2761 ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaactgct
     2821 ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt
     2881 agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct
     2941 ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa
     3001 tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt ggcggtaaca attgacctac
     3061 atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact tgctaccgcg
     3121 atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat tgccgcttca
     3181 ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc cagcccggaa
     3241 gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg aatggtgctt
     3301 tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc gcagccgtca
     3361 ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt
     3421 gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc
     3481 tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa cctggttggt
     3541 actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa aaagaatacc
     3601 ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg ggtttccgtc
     3661 tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca gggcgttact
     3721 atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata cctgaccggc
     3781 gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg cggtcaggca
     3841 atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc caatcaggtg
     3901 taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc ttgcggattt
     3961 atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa attcaaagaa
     4021 atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag cgatatcact
     4081 aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga aaaatagtgg
     4141 ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac gcggcgcaac
     4201 tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc agggctgata
     4261 agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga ttgctcgatt
     4321 tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat tatgtttata
     4381 aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat aaaagttttg
     4441 gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt aaattaggtc
     4501 gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta atggttggca
     4561 gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg gctttaacgc
     4621 caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact gattcaacaa
     4681 ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat aaacatcgcg
     4741 cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct ctgggggcat
     4801 cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg
     4861 acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc gaaggtttaa
     4921 gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg
     4981 cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg
     5041 tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa
     5101 tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg ctgacatttg
     5161 caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt gaaggcgcgg
     5221 gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg atgaatttct
     5281 ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc gatcgcctga
     5341 attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa gaaataacgc
     5401 cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt ttatgccgga
     5461 tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg ttgcaggaaa
     5521 aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca gatgaaaacg
     5581 ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat tgccggtaca
     5641 gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa cgacattttc
     5701 tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat ggtattgtcc
     5761 tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac ttgctgtttc
     5821 gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg agtcatcaac
     5881 tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac cgaacgatct
     5941 acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt caggaacagc
     6001 gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc gatagtaatc
     6061 accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt cactttctcc
     6121 tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg agtttgcgcg
     6181 ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac ttttaaagtc
     6241 agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga gtaatccagt
     6301 tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat ctccatgcct
     6361 tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag tgacagtttt
     6421 tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt tttcagatta
     6481 ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg accactttgc
     6541 gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg gaactcgccg
     6601 ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag cgaaatatcg
     6661 gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt ttcacctttt
     6721 gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg aattgatgcc
     6781 atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc atgatcaacc
     6841 gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt cccggcaatg
     6901 ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt ttgataactc
     6961 acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg cgcgaccatg
     7021 tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt ccagactacg
     7081 cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat tatccataaa
     7141 atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag cttagcaaga
     7201 gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta aacggccgtg
     7261 gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac ccggtttaag
     7321 ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga acaaaatggc
     7381 ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg agaaggcaaa
     7441 atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct gggtcaacaa
     7501 ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa tatcaatgta
     7561 tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt ccagcgccac
     7621 gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc caggattcaa
     7681 tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt aaaattcaga
     7741 aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg attttaaaat
     7801 cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga acagttcgct
     7861 taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg caccggcgac
     7921 cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg caaggaaagg
     7981 cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac gctcggcggc
     8041 atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat tgtgtttgtt
     8101 tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca ggaaaggcag
     8161 ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac tctcaatcac
     8221 atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc ttttcggatg
     8281 tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac cataaagttc
     8341 agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga gtttttgcat
     8401 gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa tcctacggga
     8461 agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa actgtgtagg
     8521 atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata accaaaccag
     8581 gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc ttttccactc
     8641 aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt atctgaattt
     8701 gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc gcgtcaggcg
     8761 tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc cgacattctg
     8821 cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct gcgtaactcc
     8881 gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc gattattgtt
     8941 ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct ggcgcgcggt
     9001 gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc gctggatatg
     9061 tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta tgccgttgat
     9121 ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa caagacgtat
     9181 ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta cctggttgag
     9241 aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt cgttattggt
     9301 ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa atactatgat
     9361 gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga actggaaaaa
     9421 gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg taaatacttc
     9481 gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc ggttggtatg
     9541 ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca ggggatctgg
     9601 atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg taaagcggga
     9661 gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct cgcacagttg
     9721 tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt tggtcgtgat
     9781 attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc gcaatacatt
     9841 aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta tgcctccggt
     9901 tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact gcaagcgcag
     9961 ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac ggatgcctgt
    10021 aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt attggcgcag
    10081 ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga agccatctgg
    10141 aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg aaatgacttc
    10201 ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga gccgcccttc
    10261 ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata gtaaataatg
    10321 agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat caggtgagga
    10381 gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg atgtcccggc
    10441 agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca tttcgacgga
    10501 gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag cggcaaaagt
    10561 taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc aggcggcgga
    10621 tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc agaccggctc
    10681 cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca gtgaattact
    10741 cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga acaaaagcca
    10801 aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg cactgcgcaa
    10861 gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat cgcgtgcatt
    10921 tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc taacactagg
    10981 gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata tcgaatacag
    11041 cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg gactaaatac
    11101 ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct gtgcaccgtt
    11161 tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg ttcaggcgca
    11221 cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg tccgctggct
    11281 ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg agccgggcag
    11341 ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca tgctctgctg
    11401 tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta actttgaact
    11461 gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct tgctggcaga
    11521 tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc gtgagcgaat
    11581 caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc acattggtta
    11641 tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct taaaagctgc
    11701 ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac ggccagaaca
    11761 gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca gccgtggaat
    11821 gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt cggcatcgta
    11881 atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa tcagtgcaat
    11941 cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc agacgcgggc
    12001 aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg gcagggcagc
    12061 gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc acggtgcgac
    12121 actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac gatgcaaatt
    12181 cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt gcagaacggt
    12241 gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca cccattcaaa
    12301 acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg tggtcttgag
    12361 tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat cgacctggta
    12421 acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg actgttgttg
    12481 aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc cgagatgttg
    12541 ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct ccggcaaaga
    12601 gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg tagccagctc
    12661 ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac gcgccataat
    12721 attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtgg cttgaccatc
    12781 gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg gccagctaaa
    12841 cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc ccattgccag
    12901 tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc gatcccggct
    12961 gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg caatccctgg
    13021 gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc tggtttcaac
    13081 cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc gctccattaa
    13141 gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta ccgttttatc
    13201 gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca ggtctggttc
    13261 gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca gctcttcaat
    13321 taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg cgctcaggtt
    13381 atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt cgtgagcgat
    13441 accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg ccatctggtt
    13501 aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac gttcattgag
    13561 atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct gccagtgcgg
    13621 acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga tcagggcgat
    13681 atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa ctgccagcac
    13741 gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg cgacaatttc
    13801 gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca gtggctcgac
    13861 acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc agtcgtgtgg
    13921 ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca aatcatccag
    13981 cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca gcagagacat
    14041 cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt tcattggcat
    14101 cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt aaaccgccaa
    14161 ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc cccggtgtat
    14221 tcatattcag taaattcaat tttacaccgg ggcttgttta ttatgctaag gtttaattta
    14281 acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct gatattacac
    14341 cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg atcggctcta
    14401 tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg acaatcagcg
    14461 ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc gcgatggcag
    14521 gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt ggagcaatca
    14581 tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg ctggaaccaa
    14641 aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat gcagaaatag
    14701 ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt acttgccaga
    14761 cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc gagtgaacaa
    14821 taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa ttggtaaact
    14881 gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca taaggcgtca
    14941 gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg atccccgcgc
    15001 tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca ataccagcaa
    15061 gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc agatcaagga
    15121 agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca gctgttttcg
    15181 agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca tcaaggccaa
    15241 ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac aacatacagg
    15301 caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta ttaatcgctg
    15361 aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta acataaccgt
    15421 taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga atgaaatgca
    15481 gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg gaaagggatt
    15541 cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc acaatatatg
    15601 tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa tgccggaaag
    15661 agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat agagaaattt
    15721 tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct tattaattga
    15781 attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt gtttattgtc
    15841 actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt taatgcgata
    15901 aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca cgtccacgct
    15961 acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat cgcggtccat
    16021 gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg tcgagagcga
    16081 gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta acgtgccgaa
    16141 atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca gtgtggcttg
    16201 ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg gcgtcgtttt
    16261 gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat cgctatcgag
    16321 agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac aaatggtcat
    16381 gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt ctcgcaaaat
    16441 ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat gttttgccag
    16501 gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga tagtgttcat
    16561 aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac ttactatcaa
    16621 tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg aagtgttaag
    16681 gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg ctgttgattg
    16741 gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt ttcccgacct
    16801 ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc ttacgcgcaa
    16861 ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg tcgctgtggt
    16921 atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg tgttggggga
    16981 taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga tgacgaccgc
    17041 catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa tcgaccaggt
    17101 cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc gcctgttgtc
    17161 gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata gaattcactt
    17221 tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt gccgctttgc
    17281 ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg gtaaagtgaa
    17341 tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca tggttgagca
    17401 ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc agtggcgcac
    17461 ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga ccatgggtgg
    17521 tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa tcagcctgaa
    17581 tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc cgatagctga
    17641 caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg atgcgacgac
    17701 ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc tcctttgcta
    17761 tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact gaggcaatta
    17821 acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt gtatgcgttt
    17881 tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat aacagcaagt
    17941 gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg acacctaccg
    18001 ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg aaatagggca
    18061 ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg gtcagccaga
    18121 tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg gatggcgcag
    18181 cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca gccaggaacg
    18241 gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa acgatcaaac
    18301 cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga ccggccattg
    18361 acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa agcagcgtta
    18421 ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg ccaacatcac
    18481 gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg aaaacccaca
    18541 gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt gcaagtccgg
    18601 tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca acaaacagac
    18661 ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg gtttgcaccc
    18721 cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact atcgattgcc
    18781 aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag cttaacgcgg
    18841 aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag tatccccatg
    18901 cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag ataccgccat
    18961 caagttcggg acgaatgcgc gtgaggatca gtatggcaaa ggccagcaat aaaatgccag
    19021 cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc gccatatttt
    19081 gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg gtgagtgcgc
    19141 tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag aagaataaat
    19201 attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt tacgaagaga
    19261 tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa ggcggcatca
    19321 agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga cggtcaggtt
    19381 attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt tcttcgcggc
    19441 acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag agaatttgat
    19501 gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga ctttagtgcc
    19561 atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa agaagcgacc
    19621 gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt tcttcgcggc
    19681 ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga tcacatccgg
    19741 gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa tacgctggtt
    19801 gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac ctttcgcttt
    19861 tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc cctggacggt
    19921 gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga caacaccgtt
    19981 aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga ggtcagcgac
    20041 cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa aagaggcggc
    20101 accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag cattaaaacg
    20161 accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg gtgtcagttc
    20221 tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca ggagggtgtt
    20281 cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt attgttatta
    20341 acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc atttttcata
    20401 tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt aaaaaacagc
    20461 cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta atgttaagta
    20521 gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat tatcagtctt
    20581 tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat attttgcgag
    20641 ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca gtacgttata
    20701 gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat gagcagcttg
    20761 tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga atatcatgcg
    20821 aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa cgccaaaaac
    20881 agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg cgggtcaact
    20941 ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag aagggaatag
    21001 cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg aaattgcgtt
    21061 actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc cggaattaat
    21121 gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc cgcgtatctc
    21181 acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt atcgcgccat
    21241 tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg cggccgggaa
    21301 agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg ccgccattgg
    21361 cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg aagtgaaaga
    21421 acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg aagcgggcag
    21481 cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa tggaactctt
    21541 tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc caggcaaacc
    21601 agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca gtgtgattgt
    21661 cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg aaatcttcag
    21721 tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc tgccgacgca
    21781 atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt gcaaagagaa
    21841 agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga ccgtgatccg
    21901 tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc cgcaggcggc
    21961 acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac cgtggcgtaa
    22021 atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg ttgcgccgaa
    22081 agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt attacgtggt
    22141 gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg cgatttcagg
    22201 gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta gcttccttag
    22261 ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg tgactcagcg
    22321 catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga ttagttacag
    22381 ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt tcgaaacatg
    22441 aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg ttaatcgcaa
    22501 ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg gtcattggtg
    22561 gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca gaactggtgg
    22621 cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac agttatctgc
    22681 atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa gtgttcctcg
    22741 gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc aaactgtgtg
    22801 gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac ctggcggctc
    22861 tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc ggcctgcaag
    22921 tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat ttagtcgcct
    22981 ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac tccggctggg
    23041 cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc ggtgcgctgg
    23101 tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac cgttccttta
    23161 tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat gatcaggaag
    23221 tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa aactcccatt
    23281 cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat cctgtcgctg
    23341 aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc cacccggttg
    23401 cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta ccgtatgaca
    23461 tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc gtactggtga
    23521 ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt ccgattgctg
    23581 gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa cgttcgatga
    23641 acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc cacatgctgt
    23701 ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc tgacggcctc
    23761 tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct ttgacctccc
    23821 gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag cagcataccc
    23881 accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa tgtcgaaagc
    23941 cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag attaatcagc
    24001 agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag tacctgagcg
    24061 atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag cgcaaaggcc
    24121 agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc ccaggcgacc
    24181 aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac actgtcaatc
    24241 gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg aaattttccg
    24301 ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa aaatattgat
    24361 gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc cgcgttcgga
    24421 tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg caacaacggc
    24481 tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt ccacgtcaac
    24541 tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac gatgattgtc
    24601 atcaaaatcg acactgccag tacacgtggc acacgccagc ggaccatatg ttgcaccagc
    24661 gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat ctcggcggca
    24721 aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat ttttaggcca
    24781 ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt gctaataata
    24841 atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa gaagtgaaaa
    24901 tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta ccgaggacaa
    24961 ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat ggacaatttg
    25021 ttcaggacgt tattttctac atttacccat cttcgtacct catccactat cttgctcgta
    25081 ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct tgccctatgc
    25141 gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa aatagttctc
    25201 ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta ttgctacaga
    25261 aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg gcggctttat
    25321 tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg ttaaaaaaat
    25381 cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta ttaccttgtt
    25441 tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt taaccaccct
    25501 ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac ctgaactgga
    25561 ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg gcaatcgtgc
    25621 tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc aaaatatttg
    25681 gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag gccgttaaag
    25741 ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc gccacgttag
    25801 ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt ggcctggtct
    25861 tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc ccgcgctgtc
    25921 gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga aaaccggtaa
    25981 cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc gaactttgca
    26041 ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg ctgtcacccg
    26101 gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc acttcacagt
    26161 tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga gtcactttac
    26221 gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat aacggcaacg
    26281 gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac ggtgcagctg
    26341 cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt gtcggatcaa
    26401 cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac acaaaacgca
    26461 gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt cctgccgtta
    26521 atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc gtttccagtt
    26581 gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga tcatcatgac
    26641 caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt ttatctgcaa
    26701 acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt gtacgcatat
    26761 cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac tatagacggg
    26821 acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa aaacgggcgg
    26881 caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa cggcccacca
    26941 tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta attttcaggc
    27001 gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg cagcaggttt
    27061 tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg ctttttgttc
    27121 aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct gcgctttctg
    27181 ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg ctgctttggt
    27241 ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg cagaagacag
    27301 acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac cctcaatttg
    27361 ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg gaatggcaga
    27421 cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa taaattacag
    27481 cgttgataat aacgtattgt gacttaagga aaatttagct accaataata gtagtcttga
    27541 tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca gtaataaatg
    27601 acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac gctagatgcg
    27661 ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt ggtttgagca
    27721 ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg cgtgcagacg
    27781 acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa taaatgcgcc
    27841 gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg caatactcga
    27901 ccccagatag taactgaaca gatacagcga ggaggcctag cctttagcgc gttttgcgcg
    27961 ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg agaagagtaa
    28021 cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac caaacggcat
    28081 aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca tggttccggc
    28141 tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa ccacggcctg
    28201 actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca gcgtgacgaa
    28261 cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt cacgccagtg
    28321 cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa aatggcgtga
    28381 ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac cgattgccgc
    28441 cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc gtccgctcat
    28501 gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga aactggggtg
    28561 gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg aaagcccaat
    28621 caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca
    28681 aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg catcggatag
    28741 cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca gtgaaatact
    28801 actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag gctgcacaca
    28861 atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga cgcgcataaa
    28921 ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct tgtcagtgtc
    28981 gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt gctaaatatg
    29041 cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct aatatattaa
    29101 taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc gttactttgt
    29161 tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata tttcgcaacc
    29221 gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac tgctggcacg
    29281 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
    29341 aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc
    29401 gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
    29461 gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg aaatgaacac
    29521 tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
    29581 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
    29641 cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac tggctgaact
    29701 ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt atgacgatat
    29761 tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg tgggcgaggc
    29821 aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc ctgcgtcatt
    29881 taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg atgcggtttc
    29941 tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc gtaactttcg
    30001 tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc cgaaaaatgt
    30061 gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc ttcaccatag
    30121 cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc tgaaaaccag
    30181 cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg cctgattgat
    30241 cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc tcctgccagt
    30301 atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct ggaaatcaaa
    30361 gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga agcctggcac
    30421 tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga tctgagcagc
    30481 aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc attgctggat
    30541 cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact tgagcgtcta
    30601 acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat tgtacatcgc
    30661 atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga gcagcatacc
    30721 ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga ggccttgttt
    30781 ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa cgtgggggtg
    30841 ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt ggaaataggc
    30901 cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg ctgcctcgaa
    30961 accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa tcaatccatg
    31021 agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc ggcattgcgc
    31081 ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg gcgcattctt
    31141 gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc gttaagtaaa
    31201 gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc ccttcctgcg
    31261 tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac gatggcaggc
    31321 gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct gttgcagggt
    31381 taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc aggaaagcat
    31441 aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca taacggtgga
    31501 gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag ggaaaacggt
    31561 ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg cgggatataa
    31621 acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag atgccttggt
    31681 gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta tcgcgttaag
    31741 cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca tttcaaacgg
    31801 cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg gcggctggcg
    31861 cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac aactgccggt
    31921 gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa cagctcaggc
    31981 gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca acccaggact
    32041 ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac cgctcattgg
    32101 tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca tccgcttagc
    32161 tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc gaaattaccg
    32221 acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg gtcatttcac
    32281 atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt gtcgccatcc
    32341 cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat cccaggctac
    32401 gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag acaccaccgg
    32461 gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag atcccggcaa
    32521 tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag ccgttgcccc
    32581 acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgactagt gccagttgcc
    32641 agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt aacgttaaca
    32701 acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca tagtcacgag
    32761 cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga ttaatcagat
    32821 tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag gccaacatca
    32881 tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca cgataggccg
    32941 cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg cgtggcgtaa
    33001 aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca ccttcgcgac
    33061 caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc aggcttgctg
    33121 cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc gcatgagggc
    33181 gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt ccgccgagcg
    33241 ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc agactgccgg
    33301 agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac ccggcaacgg
    33361 caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac atagtgcgca
    33421 gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac acgggcgact
    33481 attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga taaaaaaacg
    33541 tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg caatattacg
    33601 gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg attgccattg
    33661 cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt ctgctttttc
    33721 gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca gcaattcttc
    33781 gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca gtgaaccaaa
    33841 atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc gcatccactg
    33901 acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc agaccgaccc
    33961 ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg cctgggcgtg
    34021 agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg cttctggtag
    34081 aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg cggcttccgc
    34141 actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag aaaaattatc
    34201 ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc ccgcaccaat
    34261 ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga gcacacgccc
    34321 gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa cgtgaggttc
    34381 acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca ataccacacg
    34441 ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca cggatgagtg
    34501 aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga tcagcgccag
    34561 aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc gcagaattgc
    34621 ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg tagtgtaacc
    34681 gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc agacgaaaat
    34741 gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg gcattttacc
    34801 gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac cggcagcaat
    34861 ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc gcagtggcga
    34921 tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga ggaaaaacat
    34981 gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc caattccgct
    35041 aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca gtggccattc
    35101 atgccatcca tttcccattt tacacttcct ccggattagc cagataacca gtggtatcgc
    35161 cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg aaatgcgcgc
    35221 gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct tcaattggac
    35281 cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc ccctcggcga
    35341 cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg tactgtggtg
    35401 cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct tcatcaacca
    35461 ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt gtacatgcgg
    35521 ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc tggtgccaga
    35581 tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg aaactgactt
    35641 ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag ccagtacagc
    35701 ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta aaccttttcg
    35761 atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg gcggtgagag
    35821 gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc cgctttaagc
    35881 cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac ttttgcggca
    35941 atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg gataccgcgt
    36001 gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg taacacatca
    36061 atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc agcatccagt
    36121 agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc acatagccca
    36181 tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc aggtcaggca
    36241 acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca acaatcatct
    36301 cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca tgttgattga
    36361 tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt ttttcttttc
    36421 cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca tctgtccatg
    36481 taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat tctaccccca
    36541 gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg ttaccggtaa
    36601 gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt cgttgtggcc
    36661 cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc aactggataa
    36721 ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta gctatgccgt
    36781 caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg ttggcgggca
    36841 gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg accatgtttt
    36901 cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca gggcgaatgg
    36961 gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg atgacgatca
    37021 tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca ccgccgctca
    37081 tccgcgtttc tgccgggtga tttccgaaca taacgaccag tttcgtattg gcgatatcat
    37141 ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg atctgtgcgg
    37201 tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga cggtacggga
    37261 cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca tgtacagcct
    37321 cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc agggcttcgt
    37381 cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc atgggatatt
    37441 tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa catgcccgaa
    37501 cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag tacacggtgt
    37561 catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg caggaactcc
    37621 agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa aatggcagag
    37681 tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc agacttctat
    37741 ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt actcacttat
    37801 tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat attttttcga
    37861 tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg cgcagagtgg
    37921 ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg ggctcgtgcc
    37981 aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact ttggctggta
    38041 gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca atcccacgtt
    38101 tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc aggacatcaa
    38161 tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt tgcagtggga
    38221 aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact ggcagtgggc
    38281 ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc ttgctggaat
    38341 aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg tcacgaaatg
    38401 ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc atttttttca
    38461 gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac agatgttgta
    38521 accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt ccaagacgtt
    38581 tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg ctggtgactg
    38641 gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata atatcttcct
    38701 gctcaacggt catcaggtct gccagcagaa tgtcggcata ttttgccgat gaggtcataa
    38761 agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt tcatgcgttt
    38821 tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag atggactcta
    38881 ctgtcagacg gatggacgga cttgaagtag ttgactcaac gctacttaag gtcctgatat
    38941 gactgacatt aaccatgacg atactgcata tccatccagt tctttctctg ggtctcccta
    39001 tctgtatcag caagtctaca cctcaagtcc tgacacaagg ctgacacaaa tcaaggacct
    39061 tgctacgcca cagctagtcc ggagaaccaa cgggcgttac actgtccgct tcagacttaa
    39121 aggagctacc agcccgtttc tatcagtctc aacccgcagc acagaccgga ggaccgctac
    39181 aatgcgtcag agagagctcg cagccacagc taaggcattc ctactggaca atcctgaagt
    39241 cacactacag gagcttacag cgcacatacg gtccatggct gagcagttcc tgactgatgc
    39301 cagtgatgac tactggaatg gcgtagaggt ggcgacactg gttgacgaga agtccaacct
    39361 cagggaactt gcagcgacaa gagcgttgag tgtggaccag cagaggggca ttaagttagc
    39421 gctggaagtc ctgaccgcag cacagcagag agtcgacact ggtgatacct ctggtctgat
    39481 taagctgatt gatggtggcg taagccagct tactgatgat aataaccata ctgattactc
    39541 aactataggc gactctacgt cgattctgaa gaatgagcag ggggatagac cagcagtttt
    39601 cacacaggaa cgtcagttac tcactccaga gtcacgagaa tcatctgtac tttttcagga
    39661 attggtgaca tcactgctgg ctgagaaggt ccagacactg aaacaatcca gctttaagga
    39721 cttgtcagcg tccctgtgaa ccgccccggg tttcctggag agtgttttat ctgtgaactc
    39781 aggctgccag atcatcg
//
LOCUS       DAJTNW010000027        38775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNW010000027 DAJTNW010000000
VERSION     DAJTNW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO179_001506"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO179_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5531622.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="OO179_001507"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="OO179_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5531623.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="OO179_001508"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="OO179_001508"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5531624.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="OO179_001509"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="OO179_001509"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5531625.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="OO179_001510"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="OO179_001510"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5531626.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="OO179_001511"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="OO179_001511"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5531627.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="OO179_001512"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="OO179_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5531628.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="OO179_001513"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="OO179_001513"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5531629.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="OO179_001514"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="OO179_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5531630.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="OO179_001515"
     CDS             7138..7749
                     /locus_tag="OO179_001515"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5531631.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO179_001516"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO179_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5531632.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO179_001517"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO179_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO179_001518"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO179_001518"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5531633.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="OO179_001519"
     CDS             complement(11943..12302)
                     /locus_tag="OO179_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531634.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="OO179_001520"
     CDS             complement(12299..12805)
                     /locus_tag="OO179_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531635.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="OO179_001521"
     CDS             complement(12951..13514)
                     /locus_tag="OO179_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531636.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OO179_001522"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OO179_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OO179_001523"
     CDS             complement(14722..15096)
                     /locus_tag="OO179_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5531637.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO179_001524"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO179_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5531638.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO179_001525"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO179_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5531639.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="OO179_001526"
     CDS             complement(17166..18056)
                     /locus_tag="OO179_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5531640.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO179_001527"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO179_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5531641.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO179_001528"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO179_001528"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5531642.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO179_001529"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO179_001529"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5531643.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO179_001530"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO179_001530"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5531644.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO179_001531"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO179_001531"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5531645.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO179_001532"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO179_001532"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5531646.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO179_001533"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO179_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5531647.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO179_001534"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO179_001534"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5531648.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO179_001535"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO179_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5531649.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO179_001536"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO179_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5531650.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO179_001537"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO179_001537"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5531651.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO179_001538"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO179_001538"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5531652.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO179_001539"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO179_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5531653.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO179_001540"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO179_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5531654.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO179_001541"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO179_001541"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5531655.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OO179_001542"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OO179_001542"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OO179_001543"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OO179_001543"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5531656.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OO179_001544"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OO179_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5531657.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="OO179_001545"
     CDS             complement(38654..>38775)
                     /locus_tag="OO179_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5531658.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9708 c         8723 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DAJTNW010000028        38420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNW010000028 DAJTNW010000000
VERSION     DAJTNW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..74)
                     /locus_tag="OO179_001546"
     CDS             complement(<1..74)
                     /locus_tag="OO179_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5531659.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO179_001547"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO179_001547"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5531660.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="OO179_001548"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="OO179_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5531661.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO179_001549"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO179_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5531662.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="OO179_001550"
     CDS             2704..2940
                     /locus_tag="OO179_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5531663.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="OO179_001551"
     CDS             2943..3800
                     /locus_tag="OO179_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5531664.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="OO179_001552"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="OO179_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="OO179_001553"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="OO179_001553"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5531665.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="OO179_001554"
     CDS             complement(6400..7296)
                     /locus_tag="OO179_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5531666.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="OO179_001555"
     CDS             complement(7345..7584)
                     /locus_tag="OO179_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5531667.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /gene="nemR"
                     /locus_tag="OO179_001556"
     CDS             7687..8286
                     /gene="nemR"
                     /locus_tag="OO179_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5531668.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="OO179_001557"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="OO179_001557"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5531669.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="OO179_001558"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="OO179_001558"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5531670.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="OO179_001559"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="OO179_001559"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5531671.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="OO179_001560"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="OO179_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..16461)
                     /locus_tag="OO179_001561"
     CDS             complement(15253..16461)
                     /locus_tag="OO179_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5531672.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16469..16969
                     /gene="tnpA"
                     /locus_tag="OO179_001562"
     CDS             16469..16969
                     /gene="tnpA"
                     /locus_tag="OO179_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5531673.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="OO179_001563"
     CDS             complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="OO179_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5531674.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17690..18517
                     /gene="mepH"
                     /locus_tag="OO179_001564"
     CDS             17690..18517
                     /gene="mepH"
                     /locus_tag="OO179_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5531675.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18645..19226
                     /gene="sodB"
                     /locus_tag="OO179_001565"
     CDS             18645..19226
                     /gene="sodB"
                     /locus_tag="OO179_001565"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5531676.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19372..20541)
                     /locus_tag="OO179_001566"
     CDS             complement(19372..20541)
                     /locus_tag="OO179_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5531677.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="OO179_001567"
     CDS             complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="OO179_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5531678.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21095..22120
                     /gene="purR"
                     /locus_tag="OO179_001568"
     CDS             21095..22120
                     /gene="purR"
                     /locus_tag="OO179_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5531679.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22117..23049)
                     /locus_tag="OO179_001569"
     CDS             complement(22117..23049)
                     /locus_tag="OO179_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5531680.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23162..24373
                     /locus_tag="OO179_001570"
     CDS             23162..24373
                     /locus_tag="OO179_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5531681.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24664..25812
                     /gene="cfa"
                     /locus_tag="OO179_001571"
     CDS             24664..25812
                     /gene="cfa"
                     /locus_tag="OO179_001571"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5531682.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="OO179_001572"
     CDS             complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="OO179_001572"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5531683.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26708..28081
                     /gene="mdtK"
                     /locus_tag="OO179_001573"
     CDS             26708..28081
                     /gene="mdtK"
                     /locus_tag="OO179_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5531684.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28122..29378)
                     /locus_tag="OO179_001574"
     CDS             complement(28122..29378)
                     /locus_tag="OO179_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5531685.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29686..29762
                     /locus_tag="OO179_001575"
     tRNA            29686..29762
                     /locus_tag="OO179_001575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29720..29722,aa:Val,seq:gac)
     gene            29767..29843
                     /locus_tag="OO179_001576"
     tRNA            29767..29843
                     /locus_tag="OO179_001576"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29801..29803,aa:Val,seq:gac)
     gene            29951..30256
                     /locus_tag="OO179_001577"
     CDS             29951..30256
                     /locus_tag="OO179_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5531686.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30382..31986
                     /locus_tag="OO179_001578"
     CDS             30382..31986
                     /locus_tag="OO179_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5531687.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31998..32810)
                     /locus_tag="OO179_001579"
     CDS             complement(31998..32810)
                     /locus_tag="OO179_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531688.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32814..33599)
                     /locus_tag="OO179_001580"
     CDS             complement(32814..33599)
                     /locus_tag="OO179_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5531689.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33596..34264)
                     /locus_tag="OO179_001581"
     CDS             complement(33596..34264)
                     /locus_tag="OO179_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5531690.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34328..34966)
                     /locus_tag="OO179_001582"
     CDS             complement(34328..34966)
                     /locus_tag="OO179_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5531691.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34979..37080)
                     /locus_tag="OO179_001583"
                     /pseudo
     CDS             complement(34979..37080)
                     /locus_tag="OO179_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37101..37727)
                     /locus_tag="OO179_001584"
     CDS             complement(37101..37727)
                     /locus_tag="OO179_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5531692.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="OO179_001585"
     CDS             complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="OO179_001585"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5531693.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9260 a        10032 c         9405 g         9723 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctcc
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgaaga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    15481 tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg
    15541 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    15601 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    15661 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    15721 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    15781 gttgttgctg aatttgacct tccggcttaa ctgtcgctga agtctcgcca gcgtcttctg
    15841 gtttttttga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
    15901 agccacgcca gcatccagcc cgatcattga tgctgaaggg tgaaccggag ttgatacttc
    15961 actttctgtc tgaatactga tgtaccacgt accgcaggac tggctgacag tgacattttt
    16021 cacaactccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt tcggcagaaa
    16081 aatacggctg ttttcctgat cgagcttaac gtcctgcggg tagcggaatg cagcattctg
    16141 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
    16201 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
    16261 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
    16321 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
    16381 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca cctggtctta actggaattt
    16441 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
    16501 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa
    16561 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
    16621 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
    16681 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    16741 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    16801 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
    16861 catccgccag tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
    16921 tccccgccct gaaggacgag gttttacgac gcaccggata aaggactgac ggatgcccgt
    16981 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
    17041 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
    17101 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
    17161 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
    17221 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
    17281 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
    17341 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
    17401 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
    17461 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
    17521 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
    17581 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
    17641 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
    17701 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
    17761 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
    17821 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
    17881 gaaaaaagcc gccagtaaaa gtatgaccaa aagcaaaacc gcttcttctg ttaaaaaatc
    17941 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    18001 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    18061 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    18121 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
    18181 cagctcaccg cataccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    18241 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
    18301 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    18361 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    18421 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    18481 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    18541 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    18601 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    18661 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    18721 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    18781 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    18841 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    18901 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    18961 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    19021 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    19081 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    19141 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    19201 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    19261 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    19321 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    19381 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    19441 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
    19501 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    19561 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
    19621 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    19681 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    19741 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    19801 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    19861 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
    19921 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    19981 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    20041 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    20101 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    20161 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    20221 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
    20281 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    20341 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    20401 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    20461 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    20521 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
    20581 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
    20641 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
    20701 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
    20761 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
    20821 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
    20881 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
    20941 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
    21001 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
    21061 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
    21121 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
    21181 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
    21241 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
    21301 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
    21361 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
    21421 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
    21481 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
    21541 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
    21601 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
    21661 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
    21721 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
    21781 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
    21841 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
    21901 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
    21961 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
    22021 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
    22081 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
    22141 ttccggctcc cgtagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
    22201 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
    22261 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
    22321 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
    22381 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
    22441 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
    22501 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
    22561 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
    22621 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
    22681 aagttcgaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
    22741 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
    22801 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
    22861 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
    22921 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacacgat gcagctcctg
    22981 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
    23041 tgaccacata gtctgcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
    23101 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
    23161 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
    23221 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
    23281 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
    23341 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
    23401 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
    23461 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
    23521 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
    23581 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
    23641 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
    23701 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
    23761 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
    23821 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
    23881 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc
    23941 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
    24001 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
    24061 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
    24121 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
    24181 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
    24241 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
    24301 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
    24361 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
    24421 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
    24481 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
    24541 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
    24601 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
    24661 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
    24721 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
    24781 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
    24841 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
    24901 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
    24961 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
    25021 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
    25081 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
    25141 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
    25201 ggactagcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
    25261 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
    25321 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
    25381 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
    25441 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
    25501 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
    25561 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
    25621 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
    25681 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
    25741 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
    25801 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
    25861 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    25921 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    25981 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    26041 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    26101 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    26161 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    26221 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    26281 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    26341 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    26401 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    26461 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    26521 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    26581 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    26641 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    26701 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    26761 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    26821 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    26881 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    26941 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    27001 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct acatggaaaa
    27061 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    27121 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    27181 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    27241 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    27301 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    27361 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgag cctgcggtta tgaaacgact
    27421 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    27481 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    27541 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    27601 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    27661 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    27721 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    27781 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    27841 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    27901 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    27961 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    28021 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    28081 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    28141 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    28201 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    28261 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    28321 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    28381 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    28441 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    28501 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    28561 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    28621 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgcccccc cacattcacc
    28681 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    28741 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    28801 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    28861 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    28921 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    28981 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgcaggca
    29041 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    29101 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    29161 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    29221 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    29281 aactcgcctt cagtaagttg cgtcgcgcca aggacctcgc cggtcttaac aatttgcaca
    29341 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    29401 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    29461 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    29521 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    29581 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    29641 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    29701 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    29761 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    29821 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    29881 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    29941 gagaatcgct atggcaactt tgttacaact tcattttgct tttaatggcc catttggcga
    30001 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    30061 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    30121 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    30181 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    30241 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    30301 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    30361 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    30421 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    30481 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    30541 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    30601 caaaagcaag aagccagcca tctccagcgt tatggcgtta aaaaagaaac cttgcacgat
    30661 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    30721 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    30781 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    30841 acgtttgatt tagcggtgat tgccacgggt cacgtctggc ctgatgaaga agaagcaacc
    30901 agaacgtatt ttcccagccc gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac
    30961 gtgggtatta tgggaacatc cttgagcgga ctggatgcgg caatggcagt ggctattcag
    31021 catggttcgt tcattgaaga tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt
    31081 gaaaagctaa atattacgtt gatgtcgcgc acgggtattt tacccgaagc cgatttctat
    31141 tgccctattc cctacgagcc cttacacatc gtcaccgatc aggcattaaa tgctgagatt
    31201 caaaaaggtg aagagggcct tttggatcgg gtatttagat tgatagtaga ggaaatcaag
    31261 tttgctgatc cagactggag ccaacgcata gccttagaga gcctgaatgt cgattccttt
    31321 gctcaagcct ggtttgccga gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat
    31381 ctccaggaag tcgaacgcaa taaacgagaa aaacatactg ttccctggcg ttatgtcatt
    31441 ctgcgcctgc atgaagccgt acaggaaatt gttcctcatc tgaatgaaca cgaccataaa
    31501 cggttcagta aaggccttgc cagggttttc atcgataatt atgcggcaat cccttcagag
    31561 tctattcgtc gactactggc cttacgtgaa gcggggatca ttcatattct cgccctcggt
    31621 gaagactacg aaatggaaat taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc
    31681 tactcgtttg acgtttttat tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt
    31741 cctttccctg ggctacgcga gcaattacag aaaacagggg atgaaatccc tgatgttggc
    31801 gaagattata cgttacagca acccgaagat attcgtggac gcgtagcgtt cggcgcgttg
    31861 ccctggttga tgcacgacca gcctttcgtt cagggactta cggcatgtgc agaaattggt
    31921 gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt
    31981 gattaaaggt aaagctatca gcactcaaca aactgatgct gttcattaag acgatagcgg
    32041 actccggttc gaatattgtt ttcaccttca atggcgacgg cgaagcgaac tctttcgccg
    32101 tcatgatatg ccagtgcggc gcttccccct agcccgagaa taattgagtc gaccacgccc
    32161 gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg
    32221 gcaattctgg cgtttgcgcc aaagctggcg atttgcacca ggtcaccatt actggcgaca
    32281 tgacaccgtt cacccaatgt acaaacgcgt actcgcattc cggtattcgc aatccgacta
    32341 ctatctccag cgctgcttat ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca
    32401 ccggagctgc caatgtgact gttaaagccg acacttccaa tccgcacgct ataacccata
    32461 ctggcaattt gcgcggcata cccagcggtt gcgattcggg cgttatcctc acaacagccg
    32521 atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa atggtatctc tccggggtct
    32581 gtggccagtt tttgcataga ggaaacttcc tgatgcgcga agttttcatc cgccagccat
    32641 tttttccacg catactcaac cagactctcc gcccagtccg tatatccttc ctcaatcagt
    32701 gcatgacgaa tatcggcata actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac
    32761 atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc
    32821 cggtgatagc catcgaccat gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa
    32881 agataaagat gaccgaagat aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc
    32941 aataaccagt atcttacgcc aggaaacaca tctcccacgg cttgcggata gagacacaac
    33001 agccccgtca atagtaacaa cggcagcaat ccatacatga caccaacata ggcgacctgc
    33061 tgtaagggat taaatttaga ctgtgttgtt gccgggaaag gatgttcttc cccctgcata
    33121 atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt ccagccaccc ctgacgacga
    33181 atgcgatagt ggtgaccatt atccccaacg gcattgatca gcacaaagcc gagccagcat
    33241 gccagtaaca aaaatccgca aacttcatgc accgcaacca gacttttaac cgcagttgcg
    33301 cccaccatcg caaagtgatt tatcagccca cttgccagca acaatacaaa gagtaacgca
    33361 ttcgaccagt gccataatct gaccgctttg ctgtaaagat aaaccttctc accatgatcg
    33421 gtggctgatt tcttcacgcc acgagcacga agcaaggcgt gcagccccag caccaaccac
    33481 atcccaacca gcaatactcc ggcaatgatc aaccacaccg gccaaaattc gggagtgaac
    33541 tgtggcacat agttcgctaa ctggctctga aactgttcag catgttgcga cgggttcata
    33601 cattttcctt tttaatcaaa tgttgaccga accggcgata cagatgcggt tttccggcac
    33661 caggtagctg atattgatag tatttattat cctgcaacca cgcctgaatt tctgggctat
    33721 cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc
    33781 cttttgccag tcgggactca gcgcagaaat cacatttatc tgccacttta gtgacagggt
    33841 taaggtagcg aacctggtac ggacaagcgc caatgcaata actgcagccg atgcactgcg
    33901 atttttctac ccgcacgatc ccctgctcat cacgccacga tgcccccgtc gggcatacgt
    33961 cgatgcacgg agcatcttcg cagtgctgac atgactggcg aaaaaagtga tactgcgttt
    34021 cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa acggcttccc tgagcgggga
    34081 cgtggttagt tttacggcat gcgcgggcgc aaatattaca gccattgcac cgtgactcgt
    34141 catgaatcat ggcataccga acttcctttt cttgcctcgt gttcgctaac agagaagacg
    34201 cagaaccagt aaaaaagatt actgttccca tccccagaac aaatttgcgt cgagtaaagg
    34261 acatctgatt atcccctaat gcagtggctg caactgtcca ccctatccat gacatctccc
    34321 tgaaatttca ggggttctga aactgctcta cctcgaacca ttcgcataag ccataagcct
    34381 tgccataggt cataaatcgc tcgctaaaca agtacaacac cgccgtcgat gactccacct
    34441 gccgtatatc ggcatattca ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg
    34501 cttcctggaa gtaatatggc gcttgcatca gcatggcgac tttgtaagga cgaggatacg
    34561 tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt
    34621 gcaaagacat cgccgcgtag ttttcactca tggcctgcgt tgagtaatag taatcatcct
    34681 gacttcccgt catacaagcg atatccgcgt agtctccgtt ctgcttaatg ctttccagca
    34741 aaactgacag ttcctcttct gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca
    34801 ccagctgccc agccgcagaa tgctggcgaa tgaactcagc caggatttca gcaggttctg
    34861 gttcggttac cgtatcaacg gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt
    34921 caacttcgct gactttcgct aacggcaatt catcctgatg gttcatctta cccattcctt
    34981 acgcaggcag tagattgtgt gctgccagat cggcggcaat atcttccagc cccagacgct
    35041 gcaatgtttc gcgggttgga caaccaagct gtgggtccca gcccatttct ttgtagaaca
    35101 tcgtgagcga agcatgcata tcgtcacgat ccattttgtc agtaccttca gtaaagaccg
    35161 ggatctgcgg atccttgtcg aaaacccagg aacagataag atcgtgttca ttacgcatat
    35221 ctttggtttg catcggtttt accgtgtagg cacgatgcaa cgtaaagata cgctctgcag
    35281 ctaaatctaa tttttcctga gtcatctctt cgccggtgat cgctttgaag aatttggctt
    35341 aaagcgccag atcgccccgg taattacggc ttttcagcgg cgaaacggtc attggccaga
    35401 cccagttgca cagagtgacg gcgttatgca aacagacccg caacagcgac cactttgcat
    35461 atttaatttt tgcgtcattg attggcgtgt agtttttggt ttcatcgtaa gcatcttcag
    35521 aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa tggcaagccg gaaccaataa
    35581 agttgatatg ggtatgcgtc atgcagtcac ggttgaacat acagttaaca atggaaccga
    35641 cctgcgctga cgcttcattg gcatggtgaa ccggatagcc aaacggcgac cagagtttat
    35701 ttttcgcgta gccccagtac tctttaccca aattccagcg ttctgcgatg gcatatgaac
    35761 catcagccag gtgactcagc tcacccacac gatgcgccag acggtagtaa aaatctttaa
    35821 tgaagttaac gtcacccgct tccagttgat cccagcgaat ttctgcatac tcttcagctg
    35881 gcagaacacg cttgaacaca cctttgctgt aacaataagt aaagtcgcga tgcaactgcc
    35941 cgtagttaca ccataggccg tagtcatcga acaaattcag accaaccagg ttaccaatca
    36001 cacgaccatc gtctttatct tcaaaatctt tcggcccgtt cgggaagatg gtggtatgga
    36061 caaagtttgc tacacaagtg ttaccacctg tgctgggcac gccaaactct ttcacccgag
    36121 gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccgctc atttttactg
    36181 tgtatttctc tgccgccggt cctaagtcaa aaacggattt ataggtacga aagccgaccg
    36241 tattctgatt gcctggcgga atttcacccg tttcaatcgg accaccttca gccgcgcccc
    36301 aaaacagccc tttacgtgct gtccagcgtg acttgggatc tgaatactct gcccacgatt
    36361 gtggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc atcatgtaat
    36421 cattgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc ccttcaaccg
    36481 caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg ccgctgtggt
    36541 tacggctatt caacatgcca gagagaggaa taaggttttc cccagcctga ccaatagccg
    36601 ccacacaggt ttccgggctg gtcaatcgac aaatttcttc cgtcgttgcg cgcgtccctt
    36661 ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc ttcagccata
    36721 ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat ttcatttgcg
    36781 ctgcaaaaaa gccacccata tgggcatcgc gactaaattt cctttggtaa aagtagaaag
    36841 tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc
    36901 aaagactaat ttattcgctt catcgaaagg tttcgtgcct agcggtactt cgtcatacat
    36961 aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag
    37021 ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat
    37081 gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc
    37141 cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt
    37201 tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct acagccaata
    37261 caacgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc
    37321 gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg
    37381 agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcga
    37441 tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag
    37501 atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt
    37561 gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac
    37621 agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct
    37681 aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc
    37741 tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc
    37801 tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat
    37861 ttaacgccga taaactaatc ctttatggtt aggagaaaaa ataatgtgat tattctgagc
    37921 ccttaacatt gatcgttatc aattaaaatt acaaagcaag aaattataag cgaactgata
    37981 tttattatca tttgaaataa atttaactta agcgacaatc atttgtgacg ttcattgcag
    38041 aaaataaaaa taacattatc gctataaatt aaaatacata ccgaaattcg tataatttca
    38101 gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat caggatgaag
    38161 cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt tccatcgcct
    38221 gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt aatacgcctg
    38281 cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca actttaccga
    38341 caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc attctttata
    38401 cctatttatc atgccaacta
//
LOCUS       DAJTNW010000029        37619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNW010000029 DAJTNW010000000
VERSION     DAJTNW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..176)
                     /locus_tag="OO179_001586"
     CDS             complement(<1..176)
                     /locus_tag="OO179_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5531694.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO179_001587"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO179_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5531695.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO179_001588"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO179_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5531696.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO179_001589"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO179_001589"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5531697.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO179_001590"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO179_001590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5531698.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO179_001591"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO179_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5531699.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO179_001592"
     CDS             complement(5180..5422)
                     /locus_tag="OO179_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5531700.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO179_001593"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO179_001593"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5531701.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO179_001594"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO179_001594"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5531702.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO179_001595"
     CDS             complement(8339..9313)
                     /locus_tag="OO179_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5531703.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO179_001596"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO179_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5531704.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO179_001597"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO179_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5531705.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO179_001598"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO179_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5531706.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO179_001599"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO179_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5531707.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO179_001600"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO179_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5531708.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO179_001601"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO179_001601"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5531709.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO179_001602"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO179_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5531710.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO179_001603"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO179_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5531711.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO179_001604"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO179_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5531712.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO179_001605"
     CDS             23180..23383
                     /locus_tag="OO179_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5531713.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO179_001606"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO179_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5531714.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO179_001607"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO179_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5531715.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO179_001608"
     CDS             26388..26660
                     /locus_tag="OO179_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5531716.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO179_001609"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO179_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5531717.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO179_001610"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO179_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5531718.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO179_001611"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO179_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5531719.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO179_001612"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO179_001612"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5531720.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO179_001613"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO179_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO179_001614"
     CDS             complement(31907..32305)
                     /locus_tag="OO179_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5531721.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO179_001615"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO179_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5531722.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="OO179_001616"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="OO179_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5531723.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="OO179_001617"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="OO179_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5531724.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="OO179_001618"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="OO179_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5531725.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="OO179_001619"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="OO179_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5531726.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..37619)
                     /gene="dcuC"
                     /locus_tag="OO179_001620"
     CDS             complement(36261..37619)
                     /gene="dcuC"
                     /locus_tag="OO179_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5531727.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVI
                     ATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDK
                     KDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLL
                     SLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVD
                     AVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVV
                     MIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITL
                     FY"
BASE COUNT         9112 a         9414 c         9895 g         9198 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    37381 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    37441 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    37501 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattat
//
LOCUS       DAJTNW010000030        36871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNW010000030 DAJTNW010000000
VERSION     DAJTNW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            37..285
                     /locus_tag="OO179_001621"
                     /pseudo
     CDS             37..285
                     /locus_tag="OO179_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO179_001622"
     CDS             complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO179_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5531728.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO179_001623"
     CDS             complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO179_001623"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5531729.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2029..3255
                     /gene="mdfA"
                     /locus_tag="OO179_001624"
     CDS             2029..3255
                     /gene="mdfA"
                     /locus_tag="OO179_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5531730.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3296..3580)
                     /locus_tag="OO179_001625"
     CDS             complement(3296..3580)
                     /locus_tag="OO179_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531731.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3666..4481)
                     /gene="ybjI"
                     /locus_tag="OO179_001626"
     CDS             complement(3666..4481)
                     /gene="ybjI"
                     /locus_tag="OO179_001626"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5531732.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4481..5689)
                     /locus_tag="OO179_001627"
     CDS             complement(4481..5689)
                     /locus_tag="OO179_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5531733.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5773..6309
                     /gene="rcdA"
                     /locus_tag="OO179_001628"
     CDS             5773..6309
                     /gene="rcdA"
                     /locus_tag="OO179_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5531734.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6484..8169)
                     /locus_tag="OO179_001629"
     CDS             complement(6484..8169)
                     /locus_tag="OO179_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5531735.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8439..8816
                     /locus_tag="OO179_001630"
     CDS             8439..8816
                     /locus_tag="OO179_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5531736.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8846..9103)
                     /gene="grxA"
                     /locus_tag="OO179_001631"
     CDS             complement(8846..9103)
                     /gene="grxA"
                     /locus_tag="OO179_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5531737.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9263..9550
                     /locus_tag="OO179_001632"
     CDS             9263..9550
                     /locus_tag="OO179_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5531738.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9534..10256
                     /gene="nfsA"
                     /locus_tag="OO179_001633"
                     /pseudo
     CDS             9534..10256
                     /gene="nfsA"
                     /locus_tag="OO179_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10317..11219
                     /gene="rimK"
                     /locus_tag="OO179_001634"
     CDS             10317..11219
                     /gene="rimK"
                     /locus_tag="OO179_001634"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5531739.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11307..11783
                     /locus_tag="OO179_001635"
     CDS             11307..11783
                     /locus_tag="OO179_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5531740.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12135..13247
                     /gene="potF"
                     /locus_tag="OO179_001636"
     CDS             12135..13247
                     /gene="potF"
                     /locus_tag="OO179_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5531741.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13342..14475
                     /gene="potG"
                     /locus_tag="OO179_001637"
     CDS             13342..14475
                     /gene="potG"
                     /locus_tag="OO179_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5531742.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14485..15438
                     /gene="potH"
                     /locus_tag="OO179_001638"
     CDS             14485..15438
                     /gene="potH"
                     /locus_tag="OO179_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5531743.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15435..16280
                     /gene="potI"
                     /locus_tag="OO179_001639"
     CDS             15435..16280
                     /gene="potI"
                     /locus_tag="OO179_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5531744.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16340..16828
                     /locus_tag="OO179_001640"
     CDS             16340..16828
                     /locus_tag="OO179_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5531745.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16869..17996
                     /gene="rlmC"
                     /locus_tag="OO179_001641"
     CDS             16869..17996
                     /gene="rlmC"
                     /locus_tag="OO179_001641"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5531746.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18171..18902)
                     /gene="artJ"
                     /locus_tag="OO179_001642"
     CDS             complement(18171..18902)
                     /gene="artJ"
                     /locus_tag="OO179_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5531747.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19194..19862)
                     /gene="artM"
                     /locus_tag="OO179_001643"
     CDS             complement(19194..19862)
                     /gene="artM"
                     /locus_tag="OO179_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5531748.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19862..20578)
                     /gene="artQ"
                     /locus_tag="OO179_001644"
     CDS             complement(19862..20578)
                     /gene="artQ"
                     /locus_tag="OO179_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5531749.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20585..21316)
                     /gene="artJ"
                     /locus_tag="OO179_001645"
     CDS             complement(20585..21316)
                     /gene="artJ"
                     /locus_tag="OO179_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5531750.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21334..22062)
                     /gene="artP"
                     /locus_tag="OO179_001646"
     CDS             complement(21334..22062)
                     /gene="artP"
                     /locus_tag="OO179_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5531751.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22280..22795)
                     /locus_tag="OO179_001647"
     CDS             complement(22280..22795)
                     /locus_tag="OO179_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5531752.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23444..25213
                     /locus_tag="OO179_001648"
     CDS             23444..25213
                     /locus_tag="OO179_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5531753.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25424..25747
                     /locus_tag="OO179_001649"
     CDS             25424..25747
                     /locus_tag="OO179_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5531754.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25744..26574
                     /gene="amiD"
                     /locus_tag="OO179_001650"
     CDS             25744..26574
                     /gene="amiD"
                     /locus_tag="OO179_001650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5531755.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26571..27584)
                     /locus_tag="OO179_001651"
     CDS             complement(26571..27584)
                     /locus_tag="OO179_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5531756.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27683..29113)
                     /locus_tag="OO179_001652"
     CDS             complement(27683..29113)
                     /locus_tag="OO179_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5531757.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29124..30125)
                     /gene="ltaE"
                     /locus_tag="OO179_001653"
     CDS             complement(29124..30125)
                     /gene="ltaE"
                     /locus_tag="OO179_001653"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5531758.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30162..31880)
                     /gene="poxB"
                     /locus_tag="OO179_001654"
     CDS             complement(30162..31880)
                     /gene="poxB"
                     /locus_tag="OO179_001654"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5531759.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32013..32981)
                     /gene="hcr"
                     /locus_tag="OO179_001655"
     CDS             complement(32013..32981)
                     /gene="hcr"
                     /locus_tag="OO179_001655"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5531760.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTCDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32993..34645)
                     /gene="hcp"
                     /locus_tag="OO179_001656"
                     /gene_synonym="priS"
     CDS             complement(32993..34645)
                     /gene="hcp"
                     /locus_tag="OO179_001656"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5531761.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34789..35688)
                     /gene="lysO"
                     /locus_tag="OO179_001657"
     CDS             complement(34789..35688)
                     /gene="lysO"
                     /locus_tag="OO179_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5531762.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36145..36840)
                     /gene="aqpZ"
                     /locus_tag="OO179_001658"
     CDS             complement(36145..36840)
                     /gene="aqpZ"
                     /locus_tag="OO179_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5531763.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9225 a         9165 c         9448 g         9033 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaccg tgaccatacc gcgtgggcag
       61 ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc accgctgaaa
      121 aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat tgagcagcgt
      181 ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat gtgggatttc
      241 gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc tgataaccgg
      301 atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata cggtgcgtct
      361 gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata tcaaagcgtt
      421 tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg acaaccagca
      481 catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca agattaaaac
      541 aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa
      601 aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt
      661 caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga aaggtattta
      721 gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata atccacggcg
      781 tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc gcagccagtt
      841 ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac aggtaatggc
      901 tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc gcactgtggt
      961 tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg gcgtctttaa
     1021 gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc tcttcgcgac
     1081 gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact acacttttta
     1141 gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag acgcgatacc
     1201 acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc tgggcactaa
     1261 ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag tggacgccaa
     1321 gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag ccggaccaca
     1381 ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg tgatcgctcg
     1441 ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg acaaagggtc
     1501 ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc gccagcgcga
     1561 tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa agtaccgcgg
     1621 ccagcaacgg caccacggta atcaaatctt tagcaataaa aatcgccaac gagatcatcc
     1681 acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc aaattttcca
     1741 gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt cgttgagaat
     1801 aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc ggcctgttta
     1861 cggtgggtga ttgtaacggg cataggttaa ataaaactta aagaaagcgt agctatactc
     1921 gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct ttctcgttag
     1981 ctgcgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat gcaaaataaa
     2041 ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg tctggtgctt
     2101 tacgaatttt caacctatat cggcaacgat atgatccaac ccggtatgtt ggccgtggtg
     2161 gaacaatatc aggcgggcat tgattgggtt cctacttcga tgaccgcgta tctggcgggc
     2221 gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg ccgtccggtg
     2281 atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg caatattact ggcgcaaaac
     2341 attgaacaat tcaccctgtt gcgcttcttg cagggcataa gcttctgttt cattggcgct
     2401 gtgggatacg ccgcaattca ggaatccttc gaagaggcgg tttgtatcaa gatcaccgcg
     2461 ctgatggcga acgtggcgct gattgctccg ctacttggtc cgctggtggg cgcggcgtgg
     2521 atccatgtgc tgccttggga ggggatgttt gttttgtttg ccgcattggc agcgatctcc
     2581 tttttcggtc tgcaacgagc tatgcctgaa accgccatgc gtataggcga gaaactgtca
     2641 ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga acggccgctt tgtggcgggg
     2701 gcgctgggat tcgttagcct gccattgctg gcgtggatcg cccagtcgcc gattatcatc
     2761 attaccgggg agcagttgag cagctatgaa tatggcttgc tgcaagtgcc tattttcggg
     2821 gcgttaattg cgggtaactt gctgttagcg cgtctgacct cgcgccgcac cgtacgttcg
     2881 ctgattatta tgggcggctg gccgattatg attggtctgt tggtcgctgc tgcggcaacg
     2941 gttatctcat cgcacgcgta tttatggatg accgccgggt taagtattta tgctttcggt
     3001 attggtctgg cgaatgcggg actggtgcga ttaaccctgt ttgccagcga tatgagtaaa
     3061 ggtacggttt ctgcggcgat gggaatgctg caaatgctga tctttaccgt cggtattgaa
     3121 atcagcaaac atgcctggct gaacgggggc aacggactgt ttaatctctt caaccttgtc
     3181 aacggaattt tgtggctgtc gctgatggtt atctttttaa aagataaaca gatgggaaat
     3241 tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga tcaggcattc tcgaattaat
     3301 ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc acctacgctg gcaagatggc
     3361 gaacataagg atgcgggcgg taagccggag ctggcgcagg agccacatag acggtttgcg
     3421 gcacagtcgc gacgctgacc gcttgtggaa cgctgactga atcaggaacc acaaccactt
     3481 tgtatccact cggcacatcg acggtgacac tttgcgccat cgccacacca gtaaagccca
     3541 ttaaaagcgc gcccaacagt agacaatttt tcataatcat ttcgcctgaa ataatgcagc
     3601 gggttaattt ccgcgaatta tgcagccttc attcaggcat tgtgttgccg ggcgtgctgc
     3661 cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt tatcgatcac atccagtacg
     3721 ccttcacggt tattgtagcc tgcccggtat tttgccgctg cgacgactgc gctgccggca
     3781 ttttccattg caaaactaaa gcctgcctga cgcagcatct caatatcgtt accgccatcg
     3841 ccaaagacca ccacttcgct gtcgtctatt ccccataatt tctgcagttg gcgaaggcca
     3901 ttggctttat gtacgccggg gataatcaga tcgatgctgc cgttgccggt gtggactggc
     3961 accataatat cgccgatggc ctcatgtaat gctttttgta cttgtggaat cagttcatcg
     4021 gaaagattca ggccaaactt aaagaaaata tcttctaagt tgtcaaagtt atcgacgtat
     4081 tccagacggt gataatacat ttccgccacc gttttcatgg catcgtcata ttttttgagt
     4141 gtataggcgc tattttttcc gcaggcaata atttccactt ccgggcgcgt cagcaaatgt
     4201 tccacgacag tagcaaacgc atccttcgac agctcgccat taaaaacatc tttgccttca
     4261 ctcattaccc agccgccgtt ttcagccaca aaggcaattt cattcgcaat ttcagggaag
     4321 aaagagatca actgataata ttgattcccg ctggcgacca caaagcgaat gccttgtgct
     4381 ttcatttgct gatactgagc cataaaccgc tcacggttat aggttttttg atcgcttaag
     4441 aaagtaccat ccatgtctac cgcaattaat ttaatgctca tcaactattc tccatcgccg
     4501 tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac aatagccgcg agaataacca
     4561 gcgccagtac aaccagcatt gcactacgta atccataatg ttcgccgaga tagcccagca
     4621 gcggcggccc gacgaggaaa gccagataac cggtcgttgc taccacactg acgcgggttg
     4681 gtgcatcggg gccggtatcg ctggcggcag aaatggtcag cgggaagccc agcgaggcac
     4741 ccagtcccca aagtacaaca gacactcctg cgacccaggc gctatcgaca aaaataatca
     4801 gcccaatacc caacgccccc attagtgcac tggcccgaac cacggcaacg cgactgtaac
     4861 tgtcgatgaa ccaaccgccg gtgaagcgtc caacggtcat ccccagggta aaaccggcat
     4921 aaatcagtga gccggaagtg gggctaaaac cgtgaccatc aaccattaat aagggtaacc
     4981 agtcgttggc agaaccttcg gcaaaggcca tcgccagcac tacaacacca atcaacagca
     5041 actggatatc gcgataaaaa ggaatgcctt tttcgccatg ctgggtgcca tcggcagcat
     5101 ttttgcccgt accgtcaggg attgcctgaa tggcgatata aataggcgcg atacctacca
     5161 gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa ggccgtcagt gccatcccga
     5221 caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg catcatcggc aaaaccgttt
     5281 tattcatttc tcgctcaacg gcggcacctt caacgtttat cgccacttcc gcagaaccaa
     5341 aacttgcccc aaagacgccg agaccaacgg caaagagcag gggcgatgtc agccagagtg
     5401 ccagacttaa tatcatcatc ccgatcaatg cgcaggacat cgtgaccagg atgacattac
     5461 gtgtcccaaa gcgtttcact aaccacgccg agcagagaat accgctcatc gaaccgatcg
     5521 acagaccaaa gagaacaccg cccatttcag cgatcgagac agagagaata tcgcggatag
     5581 caggcgtacg ggttgcccag gacgccatta acaggcctgg caaaaagaag aacataaaca
     5641 gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt tacggtcata gatcacgtca
     5701 aaataagaag agggaagaca acattagcaa ggttgtgtac atttgtacac aattgcagac
     5761 agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc gagaaaaaat tatccaggcc
     5821 acgctggagg cggtgaaact ttacggaata catgctgtta cgcaccgcaa aattgctacc
     5881 cttgccgggg taccgttggg atcgatgacc tactattttt caggaattga tgagttgttg
     5941 ctggaggcgt tcagcagttt tactgagatt atgtcccggc aatatcaggc attttttagc
     6001 gatgttagtg atgctcaagg cgcatgccag gctatcaccg atatgatcta cagctcacag
     6061 gttgcaacgc cggataatat ggagctgatg taccagctct acgcgctggc tagccgaaaa
     6121 ccgctattaa aaacggtaat gcaaaactgg atgcagcgca gtcagcaaac gctcgaacaa
     6181 tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta ttgaggggat gacgctgcat
     6241 tttgtcaccg accgtaagcc gctatcgcgc gaggagattt tgaggatggt tgagagggtt
     6301 gcagggtagt agataaattt tagataacaa aaaacccatc aaccttgaac cgaaatggcg
     6361 gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact aattaagact
     6421 gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag ggcaacttca
     6481 gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac
     6541 gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgc gagctgtatc
     6601 actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag
     6661 tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca cgggcacaag
     6721 actgacaatt aatccggcaa tcaacatctg accgccaatc gcgcccaggc cgttattaat
     6781 accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga actctttcac
     6841 catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac gcataaagcc
     6901 cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac tgaatgtgct
     6961 gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc aaaatgccag
     7021 caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga tggtttttac
     7081 gcgacgggca tcaccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc
     7141 tatcggcatc tcaatctggc tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa
     7201 cttcagttgt gcgagacgtt tacctacagc gttatggttt ttaacgacca cttcttcagt
     7261 gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac ggaagctggg
     7321 atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag
     7381 cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca
     7441 gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag
     7501 ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt tggcatcagt
     7561 gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa
     7621 tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc
     7681 atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca tgccggaatg
     7741 atgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc ctgctaacat
     7801 accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag
     7861 cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcac gaaaaaaaat
     7921 ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag
     7981 attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa
     8041 aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga gacatagccc
     8101 aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt
     8161 tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tggtaattta
     8221 cgataatgtg ttttaccaga attcagggcg cagattcatt cagcgcacct aaacgatagt
     8281 aaagtaacaa tatattttac tagtgtaatc acattaggta tcaacggcta tatgaattgc
     8341 gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc cacactggcg
     8401 aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa caacgttggg
     8461 cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca
     8521 tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc
     8581 ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct ctatttggcg
     8641 caatgctggc ggcaccctgt tcaatgctca ttatgcggct gtttttttca ccgacgcgct
     8701 cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac
     8761 tgtgtttctt atttatcagt agtttgttta aaccacagca cagaaaaaat cagtaaagcc
     8821 ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ttctttcacc catgcagcaa
     8881 aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg
     8941 gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga atatctacat
     9001 actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct tttgcacgca
     9061 cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg cattatttct ctcctcatag
     9121 atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt aacctgttgc
     9181 attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt agactgaagg
     9241 ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag gcgtgttgat
     9301 actggaattt atcggaatga tgctactggc ggtggcgctg ctgtcggtaa gcgactccct
     9361 gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt ttctcggtgt
     9421 tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac gtcttgcccc
     9481 acagctgatg aaccgtccac cgcaatattc acgttcagaa agagaaaaag ataatgacgc
     9541 caaccattga acttatttgt ggccatcgct ccattcgcca tttcactgat gaacccattt
     9601 ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc gacgtccagt tccagttttt
     9661 tgcagtgtag tagcattatt cgcattaccg acaaagcatt acgtgaagaa ctggtgacgc
     9721 tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact
     9781 ttaaccgcca tttacagatc tgtccggatg ctcagctcgg cctggcggaa caactgttgc
     9841 tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg gaatcgctgg
     9901 gattgggcgg ggtatatatc ggcggcctgc gcaataatat tgaagcggtg acgaaactgc
     9961 tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg cctgcggata
    10021 atccggatct caagccgcgt ttaccggcct ccattttggt gcatgaaaac agctatcaac
    10081 cgctggataa agacgcactg gcgtagtatg acgagcaact ggcggaatat tacctcaccc
    10141 gtggcagcaa taatcgctgg gatacctgga gcgatcatat ccgccgaaca atcattaaag
    10201 aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg cgctaaaacc
    10261 gctacgtcga tgtatgatac gcgggctttt gaccaggtct gacagagagg tgcagggtga
    10321 aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg cgtgaagccg
    10381 ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac atgaacataa
    10441 atcctgcggc gtcttctatt cactacaaag gccgcaagtt accccatttt gacgcagtga
    10501 tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc cagttcgaga
    10561 tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt gacaaattgc
    10621 gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc attgcgcatt
    10681 cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg gtggtcaagt
    10741 tggttgaagg cacgcaggga attggcgtcg tgctggcgga gactcgtcag gcggcggaaa
    10801 gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa tatatcaaag
    10861 aggcgcaagg gtgcgatatc cgctgtctgg ttgttggtga tgaagtggtc gctgcgattg
    10921 aacggcgggc gaaagagggc tattttcgtt ccaatttgca tcgtggcggc gcggcaagcg
    10981 tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga acgatggcgc
    11041 tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg atggaagtga
    11101 atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg tatcgatatc gcgggtaaaa
    11161 tgatccgctg gatcgaacgc cacgctacga cagaatattg cctgaaaacg ggtggttagt
    11221 cgcaatcaca ttactgatca tggttttgcc tgcgcttttt gcgtaagctg tgccggtctt
    11281 tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt ctggatacgc
    11341 tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac tgtcaggctc
    11401 tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc gatctgatcg
    11461 ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta ttgccgctgg
    11521 cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt cttgatatgc
    11581 aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg cagggcgtaa
    11641 cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg atggtcattc
    11701 ttgaagctaa tgcccatcaa ctgctgttac cgactgacga tgaagggcaa aacaacgtta
    11761 ccgaaaacta ttttctccac tgataattcc tttcgagcac gcagtcgctg ctgcagtgac
    11821 tgcgcgctgc aaaattatct gctgttttta accttttctt aaagattatt tcacttctct
    11881 tgtgtcgatt tggctttatc acatagagca aatacgcata aaaatttgtt aaataccgtt
    11941 ttttaatccg agctatagtc tcaaaccctg gctaaagtta ttcttgcgat gcttctatat
    12001 agcgagcagt gctggccggg agagagttct cttttcttac accgcgccga taaaaaaata
    12061 tgcacgttta ttgcatatct ttcagtgtga caacttttgt tcgtttgtta acgaactttc
    12121 agaaggaaag agatgtgacc gccttaaata aaaaatggct atcgggtctg gttgcgggtg
    12181 ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc cacatttata
    12241 actggtctga ttatatcgcc cctgacacgg tggccaattt tgaaaaagaa accggtatta
    12301 aagtcgtcta cgatgttttc gactctaacg aagtactgga aggcaaatta atggcgggta
    12361 gcaccggctt tgacttggtg gttccatctg ccagctttct agaacgccag ttgactgcgg
    12421 gagttttcca gccgctggac aaaagcaaat tgccggagtg gaagaatctc gacccggagc
    12481 tgctgaagct ggtcgccaaa cacgatcccg acaataaatt tgctatgccc tatatgtggg
    12541 cgacgaccgg gattggctat aacgttgata aagttaaagc tgtgctgggc gaaaacgcgc
    12601 ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct ggaaaaactg aaaagctgcg
    12661 gtgtctcttt cctggatgcg ccagaagaag tttttgctac cgtgttgaat tatctcggca
    12721 aagatcccaa cagcactaaa gcggatgatt acaccggacc ggcaaccgat ctgctgttaa
    12781 agctgcgccc gaacattcgt tatttccatt catctcaata cattaacgac ctggcaaacg
    12841 gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca aaccgcgcga
    12901 aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc aaaagaaggg gcgatggcgt
    12961 tctttgatgt attcgccatg cctgcggatg ccaaaaacaa agacgaagcc tatcagttcc
    13021 tgaattacct gctgcgcccg gatgtagtag cgcatatttc cgaccatgtg ttctatgtca
    13081 acgccaataa agcagccacg ccgctggtga gtgcggaagt ccgtgagaac ccgggtattt
    13141 atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa agtgcaggac ccgaaaatcg
    13201 accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc gcagccttag
    13261 atgccgggcg ggcgcaccac acccgccggc aattcgcacc atcatggtgc gcttgcacac
    13321 attcaatgcc ggagagcagc cgtgaatgac gctatccctc gcccgcaggc gaaaacccgt
    13381 aaggcgctga cgccgctatt agaaatccgc aacctcacca aatcctacga tggtcaacat
    13441 gcggtggatg atgtcagcct gaccatctac aaaggtgaaa tcttcgcgct gctgggcgca
    13501 tccggctgtg gcaagtccac gctgctgcgt atgctggcag gtttcgaaca accttctgcc
    13561 ggacagataa tgcttgatgg cgtcgatttg tcacaggttc cgccttacct gcgccccatc
    13621 aatatgatgt ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca gaacatcgct
    13681 tttggcctga aacaggacaa actaccgaaa gcggaaattt ccagtcgggt caatgagatg
    13741 ctcggcctgg tgcacatgca ggagttcgcc aaacgtaaac cgcatcagct ttctggtggt
    13801 cagcgacagc gtgtggccct ggcccgaagc cttgccaagc gcccgaaact attactgctc
    13861 gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct tgaagtggtg
    13921 gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca cccacgatca ggaagaggcg
    13981 atgaccatgg cggggcgcat cgccattatg aatcgtggga aatttgtcca gattggcgaa
    14041 ccggaagaga tctacgagca tccgactacc cgctatagcg ccgaatttat tggctcggta
    14101 aacgtctttg aaggcgtact caaagagcgt caggaagatg gcctggtgct tgattcgccg
    14161 gggctggtgc atccactgaa agtcgacgcg gatgcctcgg tggtcgataa cgtgccggta
    14221 catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag agccgcccgc caatggttgt
    14281 aacttcgcgg tgggggaggt gatacacatt gcctatctcg gcgatctttc ggtgtatcac
    14341 gttcgtctga aaagtgggca gatgattagt gcccagctac aaaacgccca tcgtcatcgt
    14401 aaagggttac cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga cagctgtgtg
    14461 gtgctgacgg tttaaggagc aaagatgaat acacttgaac ctgctgccca gtcgaaaccg
    14521 ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga agcatgggcg caaactggtc
    14581 attgcgttgc catatatctg gttgatcttg ctgtttctgc tgccatttct gattgtcttt
    14641 aaaataagcc tggcggagat ggcgcgcgct attccacctt ataccgaact gatggagtgg
    14701 gctgacgggc aactgtccat cactcttaat ctcggtaatt ttctgcaact gaccgacgat
    14761 tcgctctatt tcgatgctta tctccagtcg ctacaggtgg ctgcgatttc gacattttgc
    14821 tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc ttcgacccgt
    14881 aatattttat tactactggt gatcctgccg tcgtggacct cgtttctgat ccgcgtttat
    14941 gcttggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct gtggctgggg
    15001 gttatcgatc aaccgctgac cattcttcat accaatctgg cagtttatat tggcattgtt
    15061 tacgcctacg tgccgtttat ggtgctgccg atttataccg cgttgattcg tattgattat
    15121 tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt ctttactgtg
    15181 atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt tatcccggct
    15241 gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg acagcatcat gatcgggcgc
    15301 gtgctatggc aagagttttt caataaccgc gactggccgg tggcctcggc ggtagcgatc
    15361 atcatgttgc tgctgctaat tgtgccgata atgtggttcc acaaacacca gcaaaaaagc
    15421 gtgggagaac acggatgaat aatttaccgg tagttcgttc gccctggcgg attgtgattt
    15481 tgctgctggg ctttaccttt ctctacgcgc caatgctgat gctggttatc tattcgttta
    15541 acagctcgaa gctggtgacg gtgtgggccg gctggtcaac gcgctggtat ggtgagttat
    15601 tgcgcgatga cgcgatgatg agtgcggttg gttcaagcct gacaattgct gcctgtgcgg
    15661 caacggcggc ggcgatcctc gggactattg cggcggtggt gctggtgcgc tttggcaggt
    15721 ttcgcggatc aaatggcttt gcctttatga tcaccgcgcc gctggtaatg ccagatgtca
    15781 tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt ggctggcctg
    15841 cggaccgcgg tatgcttacc atctggctgg cgcatgtcac tttctgtacg gcttatgtgg
    15901 cggtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc gatagaagaa gccgcgatgg
    15961 atctcggtgc gacgccgctg aaagtgtttt tcgtcattac gctaccgatg atcatgcccg
    16021 cgatcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg gtgatcgcca
    16081 gctttgtttc cgggccggga gccaccacgt taccgatgct ggtcttttcc agcgtgcgga
    16141 tgggggtgaa tccggaaatc aacgccctgg caacattgat cctcggcgcg gtcggaattg
    16201 tcggatttat cgcctggtat ctgatggcgc gcgcagaaaa acagcggata cgcgacatcc
    16261 aacgtgcaag acgtggctga agacactaaa atttgccaac ctggctacat aatgccgcgc
    16321 atgtcgcggc attgttttca tggaagacga aacgttggga ttttttaaga aaacatcttc
    16381 atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg gcggcgctcg ccattattat
    16441 gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg gtattggcga
    16501 gttcattcat cgcagcgtac aaacctggag tttaacgctg gtctttttaa gcagtctggt
    16561 gctggttttc attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc gctgggcgcg
    16621 ctggctatat ctgctgacac aaatcaccgc cgcaagttac ttgtgggcgg cttcgctggg
    16681 gtacggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa tcttccatag
    16741 cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg ttccctcgac
    16801 cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccctga tgatgtgaag
    16861 gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt cagtggataa
    16921 cgcagccgat tccagagcaa ctctccgcta aaaccgccga tcttaaaaat ctgctcgccg
    16981 attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg tttcgtaata
    17041 aagccaaaat ggtggtgagt ggtagcgttg aaaaaacact gctcggtatg ctgcatcgcg
    17101 atggcacgcc ggaagacctt tgtgactgcc cgctttatcc agcctcattt gcgcccgttt
    17161 ttgcggcgct aaaaccgttt atcgcccgag cggggttaac gccctacaac gtggcgcgta
    17221 agcgtggcga actgaaatac attctgctga ctgaaagcca gagtgatggc ggcatgatgc
    17281 tgcgctttgt actgcgttct gataccaagc tggcgcaact gcgtaaggcg ctgccgtggt
    17341 tacacgaaca actaccgcag ctgaaagtta ttaccgtcaa tattcagccg gtacatatgg
    17401 cgattatgga aggggagacg gagatctacc tgaccgaaca acaggcactg gcggagcgtt
    17461 ttaatgacgt accgctgtgg atccgtccgc aaagtttctt ccagactaat ccggcggtcg
    17521 ccagccagtt gtacgccacc gcgcgcgact gggtacgaca gctgccggtt aaacatatgt
    17581 gggatctgtt ctgcggtgtg gggggctttg gtttacactg cgcgacgcct gacatgcagt
    17641 taaccgggat cgaaattgcg ccagaggcca ttgcctgtgc aaagcagtca gccgctgaac
    17701 tgggcttaac tcgtttacaa tttcaggcgc tggactccac tcagtttgcc actgctcagg
    17761 gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg cggcattggt aaaccgctgt
    17821 gtgattatct ttcaacgatg gcaccgcgtt ttattatcta ctccagctgt aacgcccaaa
    17881 ccatggcgaa agatatccgc gaactgccag gttaccgcat cgaacgggta cagctttttg
    17941 atatgttccc gcacaccgcg cactatgaag tgttgacgct gctggtgaag caataaaaaa
    18001 gccacaggtg cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg gatgcggcgt
    18061 gaacgcctta tccggcctac aaaatcatgc taaatcaata cattgcagag ttgatgtggg
    18121 ccggataagc gcagcgcatc aggcaatctt acttttgtca tcagtctcaa ttactgcgga
    18181 aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc cagcgcgtta
    18241 ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatacccag gccggtgccg
    18301 aagtattgcg gatcggtcac tttttcagtc gcaacgccca gttgcggatt ggttttcagc
    18361 cattcgttta ccaccgcggt gtcaccaaat accccatcaa tacgaccatt tttcagatcg
    18421 ataaaggcat tctgataact gtcataggag acagttttca cttccgggtg ctgatcctga
    18481 atatatttct ggtgcgtcgt accgttttcc atcccaatgc gtttgccttt caggtcggca
    18541 aacgttttgt aggtatcttt tttggaaatc acgacggctg agttttcata gtagggtgtg
    18601 gtaaacgaaa cctgcttgct acgctccggg gtgatatcca taccggagat tacggcgtca
    18661 tattttctga atttcagtga cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat
    18721 tctgcctgca tttgtttgca caaggctttt gccagatcga tatcaaagcc gacaatctca
    18781 ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa attgattttc
    18841 tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac taactttttc
    18901 atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg catgggataa
    18961 caatgccatc aagtgaattt atatgcaata aacatgatta aataatttaa atgaaataaa
    19021 aaaagacgga taacttagtg agttgtccgt cttcattata agaatttatg cactatgtag
    19081 gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg
    19141 ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat gtacgcagct acattaattc
    19201 cgccgttcga atgccagtgc tttgcgctcg atcagacgca tcatcagcgt cagcaggccg
    19261 ttgacgacca ggtaaataat ccctgccgca ccgaacacca ttacatcgta ggtgcgtccg
    19321 tacaacaact ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc cagagaggta
    19381 cttttgaaca ccagcaccac ttcgttggaa taagaagaga gcgagcgttt aaaggcatac
    19441 ggcagcagga tcgccagcgt atcttttttg ctcattccca gggcgctaca ggactgccac
    19501 tgaccttctg ggatcgcacg aattgcaccg taaaacagct gcgtggtata cgccgcactg
    19561 ttcagcgata acgcaatcag cgcacataac cacggttctg acaacaaatg ccacagtgcc
    19621 ggatactcct gcaacgtcgg aaattggccc ggcccgtaat aaatcaggaa gatctgcacc
    19681 agcagcggcg taccggtaaa cagcgtgata taaccccgca ccagccacac cagcaccggc
    19741 gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac aatcagcgag
    19801 gcaacggtta gcgtcaggct ggtgtgcagc cctttcatca gctcgggtaa atactcaaac
    19861 attagctggg cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact
    19921 gactgagcag ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag gtaaatggtt
    19981 cctgggtacg agtagcaatg cttttggttt gtaacattaa atcattcaca ctaatcaaac
    20041 tgaccagcgc ggtatctttc agcagcacca gccactggtt accgaggcca ggcagcgcat
    20101 gacgccacat ctgcggcatc accagacgga aaaagatagc cgatttcgac agccccagcg
    20161 cctgaccaga ttcccactgg cccaccggta cagctttcaa cgcgccgcgc agcgtttgcg
    20221 aggcataggc ggcatacagc agtgacagag cgatgacacc acaaaggaac ggactcacgt
    20281 cgaagttctc aatgtccatc tgcaccggga tctgcacgaa cccaagattg atagtgaagc
    20341 catccgaaag cgtcagcagt aactgcgagg agccaaaata gataaacagc actaccagaa
    20401 tttctggcag gccacgcaga atggttacca gcgctgaacc tgcccacgcg acaggacgcc
    20461 atttcgctga ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca atcaatgcac
    20521 aaacggcaag gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat tcattcatca
    20581 ggaattactt ctggaaccat ttgttgtaga tggtttcgta agtgccatct ttcttcactt
    20641 tttccagcgc agtgttgagt ttctgctgca gttcagtgtt gccctgacgt accgcgatgc
    20701 cgaggccagt accgaaataa tctttatcgg tcactttatc gcccactgcc gccagttttg
    20761 ggttatcttt cagccactca gtgaccactg cggtgtcacc aaatacgctg tcgatacgcc
    20821 cgttttgcag atccagtttt gcgttctggt agctgtcata cggaacggtg gtgatttccg
    20881 ggtgcttatc cataatgaac ttctggtgtg tcgtcccgtt ctgaacgccg acttttttgc
    20941 ctttcagctg atcaacactg gtgtatttgc cttgctgacc cacaaacagg gcagagttgt
    21001 catagtacgg ggtagtaaac agtacctgct tttcacgctc cggagtgata tccatgcccg
    21061 ccatcactgc ttctacgcga cggaatttca ggcttgggat caggctgtca aacgcctggt
    21121 tagagaaagt acaggttgca tcaatctctt tacacagtgc ctgtgccagg tcgacgtcaa
    21181 aaccaacgat ctggttgttt gcatcaatcg attcaaacgg aggatagggg gcttcggtag
    21241 caaaacgaat ggtttcggca gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa
    21301 tcagaacttt tttcattgtt gttatcccga atcttagtga gagagatagt ttttaaatgc
    21361 ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt tctacgatat gaccattttc
    21421 catatacacc actcggctgg cggttttacg cgccacttca acttcgtggg tgacgatcac
    21481 ctgggtaata ttcgtttctg ccagctcacg aatgatgctg acgatttgtg ccgtaatttc
    21541 cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca tcatcaacgc
    21601 acgggcaata gcaacacgct gctgctgacc accagaaaga tgcagcgggt aacgatcgct
    21661 ataaggtttg agacgcagac gttccagcag tttttctgca cgggccagcg cctgatcttt
    21721 actcaacccc agtacacggc agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg
    21781 ccacaggttg tattgctgaa acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt
    21841 gtcagagggt gttttggtga aatcgaaatg gttgcctgca atgttgagcg taccggagcg
    21901 cggcatctca agcagattga gtacacgcag cagcgagctt ttacccgcgc cgctggggcc
    21961 aagtaacacc agcgtttcgc cctgtgggca atccagcgtg atatcgaaca gcgcctgatg
    22021 cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc attgacactc gtatactggc
    22081 agtctgatag ctattgaggt cgaagatagt acctttgaca gaataattat gcaatattta
    22141 tgctttaaaa gttaaaagca aagcgcatta ttcaataaac atagcacaaa ataacggggg
    22201 cggtggtcgg tgagcataaa tgtcggcatt cctcacgaaa tgccggataa tttacggggt
    22261 ttattggttg atcaaggcgt tagcgattct cgatggactg acggagcgta cccgccgtgg
    22321 catgaacgct accgcctaag tagcgcacat cgtcgatgac ccaacactgg ccttcctgaa
    22381 tcattaacac ttcatcctgc cagccctggt caccctgttt gagatccacc cgcaatggaa
    22441 tgttacgggc atcacgatta gggatagtcg atgcactggc aacgtgggcg ctgtctggca
    22501 aggtggttcg actggagaac ggatcgctgc ttaacaattc acgatgacta ttatcgcggc
    22561 tggcatcgct aagttgggtt gccagtttgt cactcaaata ggggcgcagg gcggtaatgt
    22621 cattgctgcg atgttgaatg cggtagtcgt agaattgttg agccacactg tcaggccctc
    22681 cctggacaca ggaaccggtg cgggggccgt tatctttata agctggcgtg acggtggtac
    22741 aggcactcag cagcagtgca catggaataa gtagagatag tgaacggtag cgcataatca
    22801 tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa acgctatcag attatttaat
    22861 acattgtttt taatacaatt ctgaaacaat ctgaacacct gattgggttt gagtccattg
    22921 ctggctaccc actcaaggtt gctcgtagct gacttactta aattacccaa acaatagaca
    22981 gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga aatgatcatt taaggtaaat
    23041 atgatagttc tgaaatggtg gtataaatcc tcctgtagag gttaagagca tctgcaagaa
    23101 gccagtttca catttataac gcgtgataaa tgaatgcggt tattgtctaa tcattacaaa
    23161 atttttcaca tgcgataaga gatcctgcga ttaagatgat tgccaaaatt acgttgtatt
    23221 tttgaaaatg agcgaaattt gatagtccca gctttgactc tcgataatat tatttatgct
    23281 acaattgtgg gtaaagaatt taagctaatg aatttgagtg aagaataaat taaattgctt
    23341 cagtatttcg ttaattaata taaatacaaa aggcgttgtg cttttataat tataaagcgc
    23401 gaggagttcg gtggattatc gtttttcttg ggtgattttt aatatgataa acaatatatc
    23461 tgatcaagca tcatcatttc caggcacaca actaaatcaa agtgataatt tcctggattc
    23521 actgagggaa tttttcgcga tattaaattc cagtcgtaag ggtgagttgt ctacatggga
    23581 taccatctac ttgcatctga tattagcaat aaatgctgac agtgatctca taaaaaatga
    23641 tgtgttgctc gcggaaaata ttccatcagc gaattaccaa tttaatacat tttttagcaa
    23701 tactttcgag attgatgtta aaaaatatct gggtaaatca gaagataacg aagttgaaat
    23761 aaaagcgggt aatgaaagaa tctctattgg aatacgtaat gtatcgaatg gaaaacttga
    23821 aagacaacag tttcttttcc cactggacta tgagaataaa ttacaagaac aattagataa
    23881 gtattttacg attgaatcgc acccattatt atacaggtat accattggta gtaagattgc
    23941 gaatgtgatt tttgaaaagt tatacagtcg tattgacttt aacaaagaac aatatatatc
    24001 ctttataaag gacgcattta ttcattttta tgattatagc aggcgatatg ctattagtga
    24061 gaatatcgat aaagacgctg taacaaacaa tatcgcattg atgagcactt tttatgacag
    24121 tgataatacg tcaggtgagg ttttaaataa tgattttaca gaagaagagt catttgaaac
    24181 tgcgttagat gtagagcatg caatagtgct tggatttgct gatgataatt ttgaaacaaa
    24241 acctgttcat tatcaggatt tattaacacg atttagtgcc tttcaagata ccgtgtttaa
    24301 tctgtttcct gaaatgcact cttcgcatta ccacgatatt tgtagcgtaa gtgtggatat
    24361 gacaaaggga acgcaatgca tgattcactt gatggtgaat gaagaagttt tcatgtctct
    24421 gcctgttcca gttgccacga tggtacgtga agatgcaagt aatttagtca atcttaaaac
    24481 actactcaat gatggttgtt tcattaaata tagtcatttt aatgacgtgg cattaataaa
    24541 gcaaaacata agtaatctct atttatcaca cactgttgtg aatgaaagca tattgaaaaa
    24601 atgttgtttt gaaaacggca gtttaggtga tgttaaaata acaaatagca atgtaataaa
    24661 cagtgcattc aaaaatattt cgtttagatc tgttaaaatt aataacgtaa atactcattc
    24721 tttaaaattt attaactgca attttttcaa tgttgatatg ataagagtta atctcagcaa
    24781 atgtcttttt catgaatgta gtatgcatgg tgtcaagata aaaccctggt tgcctgtaaa
    24841 atggacaaaa gaattaataa gtgattattt gtatggctgt ttactatctc tgtactctat
    24901 ctgtgcaagg gatatatata atatgaacgc cgggaataac gtgaaggttg ctgcggatgc
    24961 tttccttgaa attattttct ctcttaaaaa taaatattgt attaaactat taagcgctca
    25021 ggatcgggcg tttatatacg agtttgcaag aatgatcttc gcttatatta atgacaaaag
    25081 cattgagatc ttattacttt cttgttttgc ggcggctgac caaaaagcaa tccagcggta
    25141 tagacctcag tcacaagatg gggaagactt taggagtcat ttacaatata agttgcctct
    25201 ttccgctcat taatgttgtt ggtgcaacat aataagacag gtaagcggta ccctataatg
    25261 aaaccaatat cgggttatgc aacggataat tgtaaagact taagtgggtg tttttgacac
    25321 aatgagatga tttcctaaac tgatcaaaac cttcttccca acatggcata tcagtccgat
    25381 aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac
    25441 cccaactctg gaaggcctga ccatcgttga atattgcggt gtggtgaccg gcgaagcgat
    25501 tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
    25561 ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg aggaattagg
    25621 ctcccaggcg cgggcgctgg gggccgatgc cgtagttggt attgatatcg actacgaaac
    25681 ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
    25741 tcgatgagaa gagtcttctg gctggtcgct gccgctctgt tactggcagg gtgtgcaggc
    25801 gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacacca ggcgcaggcg
    25861 gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg
    25921 ctggcgacac tgactgataa gcaggtcagc tcgcattatc tgatccctgc ggtgccaccg
    25981 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat
    26041 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
    26101 ctggaaaacc gtggctggca aaaatcatcc ggagtgaaat attttgcccc gtttgaacag
    26161 gcacagattc aggcgcttat tccgctggcg aaagatatta ttgcccgtta tcacatcaag
    26221 ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga tccggggcca
    26281 ttatttcctt ggcagcaact ggcgcagcag gggattggtg cctggccgga tgcgcagcgg
    26341 gtgaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc gtcattgctg
    26401 gagcttttgg cgcgctacgg ttatgacgta aaacctgata tgacaccgcg cgagcagcgg
    26461 cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat
    26521 gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga ttagcgcggc
    26581 agttttccgt ggtcgcgtag ccaggcggca gttttctcga tgccttcatc cagggtgatg
    26641 accggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc
    26701 aacttggaga cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc
    26761 tccatgctgc gggcgatcat atccagcatc gggtaaggga cggaacgaat acgacagtca
    26821 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg
    26881 gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac
    26941 attgcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt
    27001 aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg
    27061 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
    27121 gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag
    27181 cggtgagggc gaaaatcttc tttaatattg cgatggtggt gataatcgaa gtacagggag
    27241 ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagcccattc acccaggcgg
    27301 cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag
    27361 ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc
    27421 agctcggtca gatccgtcgg aacaaactct gcgcccattt tttccagcaa tttacccatt
    27481 gcttcgttgc gaccgctcgc acgcacgctg atgcctttct ggcataaaaa ctctaccgcg
    27541 tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt
    27601 tgagaaaatg acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa
    27661 agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt
    27721 ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc
    27781 ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtacgatag tcacctttat
    27841 cttccagggt aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa
    27901 gttgcttttt cggttcaacg acaatcactt tccagctatc caccgcatcg ccggtctgta
    27961 aatattcgcg ctccgggcgg cctttcgcca gattatgacc gatcgcgcgg tccatcaacg
    28021 cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca
    28081 ccaccagcca taaagccgca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg
    28141 cgaaataacc gtactccggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc
    28201 tggagttgac cagtttttct tcctctttca acgtgctacg taccgcgtca tcgaaagcga
    28261 tcagccgttg tgggatgagt gcacgtagcg cggtatcatc tgccagcaga tcgtgtttca
    28321 gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc
    28381 acaccgaaat ccagcgggtg gggaggggga tggggatcaa ccagcggcgc ttaccgctca
    28441 ccgccataaa atgttcaaac tgttgctgat aactgagcac ctctggtccg gcggcttcga
    28501 agatgcggtg ttcgttggct ggatggtcta acagcgccac cagatagtgc agcaagtttt
    28561 ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac actggcaggt
    28621 tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg ataattccgg
    28681 cccgaagttc ggtcactggt acattcgctt cacgaagaat gtccgccgta gcctgacgag
    28741 ctcgcagatg atccgactgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt
    28801 gcttaactgg tacttcacgt agcgcatcgc ggacgttgag agccagctgg cgctcctgag
    28861 cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagacggta tcgatatcct
    28921 gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacgtttg
    28981 ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgctaggatc tgatgccctt
    29041 gctggctgag tgtgcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa
    29101 tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact
    29161 tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg
    29221 ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt ttcttccccg
    29281 acgcgaacaa acagcatatt agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt
    29341 tgctccgcca tccaggcggc gttgtcgtga tcttcctgca accgtgcgac gttatttttc
    29401 agggcataca tcccggcggc agccagaatc ccggactgac gcatcccgcc accggccatt
    29461 ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgacgagtaa tgaaccgact
    29521 ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct
    29581 ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc
    29641 gccagattgc gctcgcgggt aaattcccat gcttctttca ggtattcacg cggcagcact
    29701 ttgccgttgt gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg
    29761 ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg
    29821 ggttgcggct gaatactgcc cagcaccgct gcgccaccgg cttcaaacag atagttatgc
    29881 gcggcctgac cgacaatata ctcttcgcca cgttcgcagt gactgagcag agcgaccagg
    29941 ttggcctgag tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca
    30001 tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg
    30061 gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca
    30121 atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagttcgtt
    30181 ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa ttgcgcgcaa catatacagg
    30241 ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa tggctaattc ttctttggcg
    30301 accaccacat ccaccaacac cggaccgtcg atagagaagg cgcgttgcag ggcttcatca
    30361 acttcagagg ctttttctac acggataccc gtaatgccgc acgcttcggc aatgcgggca
    30421 aagttggtgt cgtgcagctc agtaccgtct gtcaggtaac ctccggcctt catctccatc
    30481 gccacaaagc ccagcacgct gttattaaag acgataattt tcaccggcag tttcatctgc
    30541 actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc
    30601 acctgacgtt ccggctcggt tgcctgcgca cccaacgcct gcggcatggc gttagccatc
    30661 gaaccgtggt taaacgaacc taacaggcga cgcttgccgt tcattttcag ataacgtgcc
    30721 gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg cgtcatcggc ggcaaaatga
    30781 ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg
    30841 tccagccctt tgcgggcatc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg
    30901 gttttttctt ccaccagtgg cagcaatgcg cgcagggttg atttgatatc gccgaccagc
    30961 gccatatcta ccttgctatg cgcgccgatg ctggctggat taatatcaat ctgaataatt
    31021 ttggcatcgg tcgggtagaa cgcacggtag gggaactgag taccgagtag cactaacgtg
    31081 tcggcgttca tcatggtatg gaaacctgac gagaatccga ttaacccggt cattccaaca
    31141 tcatacggat tatcgtattc gacatgctct ttaccgcgta gggcatgaac aataggtgct
    31201 ttaattttcc cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac
    31261 atcagggcga tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct
    31321 tccggcatca cgactggctg tggcgcatga taccagtgcg tggttgcccc ttctggcgca
    31381 ggttttaacg ccacgtcgcc cggtaataca acaacggaaa cgccacggtt aagcaccgct
    31441 ttgcgcatag caattgccag cacttgtggg atctgctccg ggctggaaac cagctcgcaa
    31501 tagtgactac attcgcggaa tagctcttgt gggtgggttt cctggaaata gccgctgcca
    31561 atttcgctgg agggaatatg agcggcaatc gccagtaccg gaacgttatt gcggtggcaa
    31621 tcgaataggc cgttgatcaa gtgcaggttg cctgggccgc acgatccggc acagaccgcc
    31681 agttctcctg aaagttgtgc ttcagcgcca gcggcaaacg ccgccacttc ttcatggcgg
    31741 gtggacatcc actcgatggt gcccatgcga ttaagactgt cgctaagacc gttcagagag
    31801 tcgcccgtga ctccccagat gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa
    31861 gctgcaaccg tttgtttcat ggttctccat ctcctgaatg tgataacggt aacaagttta
    31921 gttcatctga cggaggggga agggatggga gagaaaggag gcactaacgg ttaaatagcc
    31981 cgatgaaagg aatatcatcg ggcataaggc gattatgcga gaaccaaatc cccctgcgga
    32041 tggcaggagc aggccagtac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta
    32101 ctgctcaccg tatattcacc ggaaaccact tttgtcttac agcagccgca aacacccgca
    32161 cggcaggcag cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca
    32221 accggggcgt aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc
    32281 gcttctgcta ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc
    32341 acttcctgct ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa
    32401 gctaagtcag gtacatctgc cagcagttcg cgagtgagac gaccagcgat aaagccttcg
    32461 gtaacgttat tttctgccac cagcgttacc ggatagttac gccactcatc ggcgaaaata
    32521 acatcctgcg gcgtacgcac gttgtagatc acccgcacat cggcctgtgg acggttcttc
    32581 gcaagccagc gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg
    32641 aatttatctt ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga
    32701 taatcaccgc gttttacatc gcacgtcagc cactgggagc cgacaccgtc atcaatccgc
    32761 cgcacggtca gggtgatata ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc
    32821 agcgtttccg ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg atatgggtag
    32881 tgatcgtggc aaatcaggga aatcgtccac acatccggcg tttcttgcgt aatgtgatga
    32941 acctgtatcc gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgt
    33001 tcaacagttg cttcatgtct tcttcaacag tggtgataga acgcaggccg aatttctcgt
    33061 tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat
    33121 ttttcacgcc cagagaaagc agcgtcagca gaatgacaat cgctttctgt tcaaaccagg
    33181 agagcaccag cgacagcggc agatcgttca caccacagcc cagtttctct gccagagtga
    33241 cagccagaat aattgctgag taagcatcgt tacattgacc tgcatctacc agacgcggca
    33301 gaccttcgat atcgccaaac tcaagtttgt taaagcgata tttaccacag gcgagggtca
    33361 ggatcaggca gtcatccggc acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc
    33421 gtgcgccgtc acagccacca agcaggaaga tatgacgcag tttttcacgg ctaaccagat
    33481 caatcagcgt atcggctgca ccaagcagcg tctggcgacc aaagcccacg gtgatcaggt
    33541 gaggaatttc gctgtacggg aagcccgcca tttgttgcgc ctgggcgatg accgcagaga
    33601 aatcttcgcc atccagatga cgcacgccag gccagccaac gatgctgcgg gtccagatac
    33661 gatcgtcata agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc
    33721 cagggaaacg agcgaactcc acttgctgat tctgccagcc gctgccgtag ttaccgacca
    33781 gatgcttgaa tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt
    33841 agacattaac gcctgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt
    33901 gaccggaaat cagaatgcat ttacccgccg tcgctttgac gttgacctgg gttggcgtcg
    33961 ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct catcactttg aagttcatct
    34021 ggccgatttc cattgaacac tcaagaagcg cgttcatatc ggcaggccag gtccccagcc
    34081 acgccatgat tttatggtac tgggcataaa tatcgttgtc gtattgaccg agaacatgcg
    34141 cgtgttccat ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga
    34201 gaatgttttc gccaatcgcc gctttatctt tgttaggggt aaattctgct gcctgacgtt
    34261 gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg
    34321 cgttggcatc tacagccagg cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac
    34381 gagcgtagcc gacaatacgc ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg
    34441 cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc gtattcacgc gcttttaccg
    34501 cccaggcaga aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg
    34561 ccgttttacc acacatcccc tgcgcgtatg agcagccgtt ttctgccgga gtacggatag
    34621 tttgttcaca ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg
    34681 tttaagggta agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag
    34741 attgatttag cgcaattttg gcggcaggga actaccgcca gagaggtatt acgcagagaa
    34801 aaaggcgatg aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg
    34861 gaccatatcc agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc
    34921 accgcataag cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat
    34981 agcaatcagt tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa
    35041 agattcggtc aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag
    35101 cgcggtattg atggggagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact
    35161 gacaaccacc accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt
    35221 catgccatta ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc
    35281 actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat
    35341 cactactacg ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt
    35401 ttcttgctga tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat
    35461 attacacagt aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt
    35521 actggcgagg ttatcgagaa acgccagact gatacccata aaaaagagaa taaggtaaac
    35581 catccagctt aatagctgat taatagcttt taacgcagct tgttggcgaa gcggaatgag
    35641 gtaacccaca atcaggggaa ccagaatgat taacagccca gaaaacatga aaatccagtc
    35701 cttgcaaaga agaagtcgaa atgcgcgatg acacactact gaaagcggaa gggcgagtaa
    35761 agttgcaatt aaaaggaaat gttatgcatc agagggggat ttttaatcct atctgagtgt
    35821 ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa agccactggc ggagggagcg
    35881 cgcagggggc agccagtcgc cgccgccccc tgcacccccg ggctctggcg aacaaaatcg
    35941 ccgctgcgcg gtaccttcag cttatcccct tcggcaaccg ggtcgggcgc gaggtaacgt
    36001 cctgtaagac gcgccctcag cccacatcca tgtgggctgc cccggccttc ggggaacgct
    36061 tcagcgattt tgacgccacc aatacccgtg cggtttgtaa tgtcgaagat aaaacaacca
    36121 gggcagataa aatgccaatg ctaattaatc acgcttttcc agcagagtcc ggtaaatcag
    36181 accaccgata atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc
    36241 ccagccgccc tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt
    36301 caccggaata ctgattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa
    36361 acctgccgga gcgaatttgt cggttgcacc gtggatcact aacaggaagc ccgcactcaa
    36421 taccagttca actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata
    36481 accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa
    36541 atacagcagc gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac
    36601 ttcttttgcc ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg
    36661 accaccagaa atatgaccaa cagcgaaggc catcgtcaga acggtcaaac caaatgccag
    36721 cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cagcactacc
    36781 acagccacca aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat
    36841 atccacctca attaaaaatt gaccctgtga a
//
LOCUS       DAJTNW010000031        36641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNW010000031 DAJTNW010000000
VERSION     DAJTNW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(157..1392)
                     /locus_tag="OO179_001659"
     CDS             complement(157..1392)
                     /locus_tag="OO179_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5531764.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1539..1615)
                     /locus_tag="OO179_001660"
     tRNA            complement(1539..1615)
                     /locus_tag="OO179_001660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:tgg)
     gene            complement(1658..1744)
                     /locus_tag="OO179_001661"
     tRNA            complement(1658..1744)
                     /locus_tag="OO179_001661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Leu,seq:cag)
     gene            complement(1765..1840)
                     /locus_tag="OO179_001662"
     tRNA            complement(1765..1840)
                     /locus_tag="OO179_001662"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:His,seq:gtg)
     gene            complement(1899..1975)
                     /locus_tag="OO179_001663"
     tRNA            complement(1899..1975)
                     /locus_tag="OO179_001663"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:ccg)
     gene            complement(2078..3463)
                     /locus_tag="OO179_001664"
     CDS             complement(2078..3463)
                     /locus_tag="OO179_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5531765.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWPYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO179_001665"
     CDS             complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO179_001665"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5531766.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO179_001666"
     CDS             complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO179_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5531767.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO179_001667"
                     /gene_synonym="wecF"
     CDS             complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO179_001667"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5531768.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO179_001668"
     CDS             complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO179_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5531769.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO179_001669"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO179_001669"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5531770.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO179_001670"
                     /gene_synonym="wecD"
     CDS             complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO179_001670"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5531771.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO179_001671"
     CDS             complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO179_001671"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5531772.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO179_001672"
     CDS             complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO179_001672"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5531773.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO179_001673"
     CDS             complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO179_001673"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5531774.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO179_001674"
     CDS             complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO179_001674"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5531775.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO179_001675"
     CDS             complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO179_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5531776.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO179_001676"
     CDS             complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO179_001676"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5531777.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO179_001677"
     CDS             complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO179_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5531778.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO179_001678"
     CDS             complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO179_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5531779.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO179_001679"
     CDS             complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO179_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5531780.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18720..19985
                     /gene="rhlB"
                     /locus_tag="OO179_001680"
     CDS             18720..19985
                     /gene="rhlB"
                     /locus_tag="OO179_001680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5531781.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20119..21603
                     /gene="gppA"
                     /locus_tag="OO179_001681"
     CDS             20119..21603
                     /gene="gppA"
                     /locus_tag="OO179_001681"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5531782.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO179_001682"
     CDS             complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO179_001682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5531783.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23758..24039
                     /gene="ppiC"
                     /locus_tag="OO179_001683"
     CDS             23758..24039
                     /gene="ppiC"
                     /locus_tag="OO179_001683"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5531784.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO179_001684"
     CDS             complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO179_001684"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5531785.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25710..26600
                     /gene="ilvY"
                     /locus_tag="OO179_001685"
     CDS             25710..26600
                     /gene="ilvY"
                     /locus_tag="OO179_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5531786.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO179_001686"
     CDS             complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO179_001686"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5531787.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO179_001687"
     CDS             complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO179_001687"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5531788.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO179_001688"
     CDS             complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO179_001688"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5531789.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO179_001689"
     CDS             complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO179_001689"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5531790.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31268..32914)
                     /locus_tag="OO179_001690"
     CDS             complement(31268..32914)
                     /locus_tag="OO179_001690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5531791.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32917..32967)
                     /locus_tag="OO179_001691"
     CDS             complement(32917..32967)
                     /locus_tag="OO179_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531792.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO179_001692"
     CDS             complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO179_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5531793.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33503..35023
                     /locus_tag="OO179_001693"
     CDS             33503..35023
                     /locus_tag="OO179_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5531794.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35048..35386)
                     /gene="maoP"
                     /locus_tag="OO179_001694"
     CDS             complement(35048..35386)
                     /gene="maoP"
                     /locus_tag="OO179_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5531795.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35505..36344
                     /gene="hdfR"
                     /locus_tag="OO179_001695"
     CDS             35505..36344
                     /gene="hdfR"
                     /locus_tag="OO179_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5531796.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36440..36515)
                     /locus_tag="OO179_001696"
     tRNA            complement(36440..36515)
                     /locus_tag="OO179_001696"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36480..36482),aa:Trp,seq:cca)
     gene            complement(36524..36600)
                     /locus_tag="OO179_001697"
     tRNA            complement(36524..36600)
                     /locus_tag="OO179_001697"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36564..36566),aa:Asp,seq:gtc)
BASE COUNT         8944 a        10035 c         9231 g         8431 t
ORIGIN      
        1 tgccaccgca cgtaaaggct ttgccggata cgatcaccca aaccagtatc cacagaacgc
       61 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
      121 caatcaaccg caggcggctt ccaaatggcc gcttttttac ttattcacca ccatcaaatg
      181 tgcctgcata atgtcactgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
      241 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagccccgg
      301 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
      361 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
      421 ctgttgcggg gaatcgatca tttctgcaat tgtttgctga tgcatattcc ccagccgata
      481 ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga
      541 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
      601 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa
      661 aatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
      721 ctgaatatca ccaggcgcat gtagtttcag tccatcgcgg acagctgttt catcagccag
      781 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
      841 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
      901 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
      961 tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     1021 aaaattattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg
     1081 gaaactgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     1141 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     1201 ttgcagttcg ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat
     1261 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     1321 gttcagatta caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac
     1381 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     1441 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     1501 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     1561 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     1621 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     1681 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     1741 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     1801 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     1861 ggtattacta ccaccgcagc tcaagcgccg gtactaaatg gcgcgcccga caggattcga
     1921 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     1981 gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc tttttaaata
     2041 aattgctcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     2101 taccgtggcg attaagccca aaaactttat aaatcgccgt caccgccagc ataaagatga
     2161 tgccgacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     2221 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     2281 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     2341 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     2401 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     2461 ccgcaaccgg tacgccgtga cgggaaactt ttgccattgc cgccggtaac tgacggtttt
     2521 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     2581 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     2641 aagtcagtac gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga
     2701 taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacggcac
     2761 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     2821 cctggtagga cgccaccaca atacacagag cggtcaggaa ccctttccag ccacccgcaa
     2881 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     2941 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     3001 tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc cgccagattc gccaacgcca
     3061 ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccggggac caaaactgga
     3121 cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag
     3181 gccaggcggt gagatagcca aaaaacgggc tcatataacg atgcgcataa acggcgaacg
     3241 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaaaacga
     3301 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     3361 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     3421 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     3481 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     3541 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     3601 aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     3661 ttgccggtgt agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     3721 ggctgcgaaa gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg
     3781 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     3841 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     3901 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     3961 ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct
     4021 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg
     4081 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     4141 tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata tttaaattcg
     4201 gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcggca
     4261 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     4321 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     4381 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     4441 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     4501 cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct tcacgtgcca
     4561 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     4621 gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     4681 ccacaatcgc cccgagtggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     4741 gcgtaggcga gatcgccagt ccggagtggt tattcagtac ttcccaggta aagtaattgg
     4801 ctgagttcag caccatactc gggcgccccg gccacagcca ggaagggata aagacataga
     4861 aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     4921 acgccagatt ctcccacggt gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg
     4981 cttcatcgcc gctcacattc attccatagc gttttaacgc cagccagaac atgccaacaa
     5041 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     5101 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     5161 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     5221 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     5281 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     5341 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     5401 acgtaagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     5461 cgcgtttgcg taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag
     5521 aaagcaacac ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca
     5581 gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga
     5641 cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg
     5701 tgcagagcag ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     5761 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     5821 ggtgttttta tccgccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     5881 atcgtcggta gtaaacagca ctggcaaatg ctgttccgtc atatcctgcc agaacggatt
     5941 ttccctgtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc
     6001 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc
     6061 gtcaaattcc agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc
     6121 cgcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac
     6181 cacttttacc gtatcgccaa attgctgatg aacggcacgt aaagcggcga catgctcatt
     6241 gctgcggtcg ccggagttcc ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt
     6301 cgccatcata ttgagagaag ggtccatccg cgtcgggaag tacagcagtt ctccccgtac
     6361 ctttgggtgc gttttggcaa aaaagctcaa atcgccgcgg gtggcaaata cacagccgac
     6421 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     6481 ctcatacagg tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact
     6541 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg
     6601 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     6661 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     6721 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     6781 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     6841 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc
     6901 gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg
     6961 aactggctga cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa
     7021 ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg
     7081 cgcatagcgg taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag
     7141 acggtaaaac tcgctgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     7201 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     7261 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     7321 acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     7381 aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg
     7441 ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttaat caacatgatg
     7501 gcggcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa
     7561 ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc
     7621 gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc
     7681 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta
     7741 ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     7801 gagaaaccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     7861 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     7921 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     7981 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     8041 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     8101 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     8161 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagt gttcccccgc
     8221 agggcaaccg tgcagcggaa tgtaatgaaa caccgccatg atttccgctt ctttcagaaa
     8281 gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt
     8341 ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc
     8401 taacgtatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
     8461 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat
     8521 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt
     8581 ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc
     8641 ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
     8701 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttccaccac
     8761 aaacaaatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc
     8821 gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc
     8881 gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc cacgcagcac
     8941 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
     9001 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
     9061 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
     9121 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
     9181 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
     9241 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
     9301 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
     9361 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta
     9421 aagagacata gccgcgaata tcgccggacg ccgcacgtag aatcagacat tgatgatcaa
     9481 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg
     9541 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctagctaac tgacgtaatg
     9601 cgggaatatc ggtctcttgt gccacgacag caccgctatc actgacattg ttcacgggta
     9661 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
     9721 cccccgtatt tgatgccgca attttggcct gcacccgtga ccatggcgct aaggcgtctg
     9781 acgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
     9841 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
     9901 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
     9961 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    10021 tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc ggtatccagc
    10081 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    10141 ttgatggagg taatctccag ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc
    10201 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt
    10261 ggcttttctt caagcgagat agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt
    10321 tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga
    10381 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    10441 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    10501 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    10561 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    10621 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    10681 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacccttc
    10741 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcaccctg atagctgccg
    10801 tcctgcacct gcttccacca gctttcatta gccagatacc actgaaccgt tttacgcatt
    10861 ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt cgaagcatca
    10921 atggcataac gcagatcatg ccccggacgg tcagcgacaa aggtgatcag gtcacgatag
    10981 tgcgccacgc cgtgcggctt gttcgaagcc agttcttcca gcagctcgca aatggtttcc
    11041 acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc
    11101 ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg
    11161 atttgctgcc cgttgccata taccggcagc ggtttacccg ccagcgcgtt gaggatcatc
    11221 agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgatc
    11281 agcgtcggca gaccgtaggt ccgcagccag gcgcgcacca gatggtcgct gctggctttt
    11341 gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc
    11401 gagtgcaggt caccatatac ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt
    11461 ttatcttccg tcagcgtatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg
    11521 acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg
    11581 gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgtgc cagttctgcc
    11641 cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc
    11701 gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcatc gctcgtttcg
    11761 ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt tatcagaatt
    11821 tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg acattgtcgc
    11881 cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca
    11941 gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga
    12001 tgttaggctc aacaaccagc gtttcgccgc tgtgccactg ggcgatcagt tcagcgattt
    12061 ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg
    12121 cgattttcag ttcactggcg cgtttatcgg tagcagcaag gcagtcggca accgccgctt
    12181 tcacctgatc gataacccag aacggtttgt gatcgttaac ttcgcgcgcg gtacggataa
    12241 gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac
    12301 cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa
    12361 tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag
    12421 cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt
    12481 tagtgacgac acactcacct tcgaggaaaa ttttgtacaa ttcgctggcg cgggccgaac
    12541 aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc
    12601 ccggtaatac acgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct
    12661 gtgggaaagt cagatccggg cgcatctctg ctaaccattc tgccatcttc tcggttgacc
    12721 ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg
    12781 agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtag
    12841 gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc
    12901 cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac
    12961 gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg
    13021 aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg
    13081 caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg
    13141 accatcacca tacgggttat gggcgcggct catagcttga tattcgtttt cgtcttttaa
    13201 aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc
    13261 cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag
    13321 cgaaggcgct tcttcctgaa tgccgcctga gtcagtcaaa atcagccagg cgtggttcat
    13381 cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt tcacatgccc
    13441 cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg
    13501 gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt caaagccacg
    13561 accgaaactc tcacgcctat gaccggtcac cagaatcatc tttttatcgg gggcgataaa
    13621 cgggtaattt gccgccagtt ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac
    13681 ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat ccgcaacgtt
    13741 ttcacgcagc aagttttgcc gggaagtttc ggttggagag aagtgataca tcgccagatg
    13801 cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagagcgc ccgtgcgcag
    13861 accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag
    13921 cgtcgtcgtc gtatcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg
    13981 ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat
    14041 gttgagatcg tagtcaggta caatggaaaa gagtttcagt acctgatcaa gcatctcccg
    14101 atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac
    14161 caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc
    14221 gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg
    14281 agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa
    14341 tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat
    14401 agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg
    14461 ctcgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc
    14521 gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag
    14581 gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgctcaa
    14641 tactgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgag
    14701 ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc agatggctgg
    14761 ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg
    14821 tttcggcaat aagcttcacg ctgtcattga ccgcgcgagt aaagtctccg gggataaact
    14881 ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc
    14941 gctgtttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt
    15001 gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttgatcggca gaagccatgt
    15061 ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat
    15121 tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaaagtat
    15181 acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc ttcccagccc
    15241 acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc
    15301 caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg
    15361 atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt
    15421 aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga cccactccgg
    15481 gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg cggcaagggt
    15541 aatcagcaca aacgcctgac gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg
    15601 ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac
    15661 catatccatt agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt
    15721 tttgccctgg gtcgtttcga gcaggatcca gataacggta aaaccaatca gcgtactgcc
    15781 cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatgta
    15841 tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa
    15901 ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc
    15961 aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa
    16021 gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag
    16081 ataaagtttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat
    16141 ttttacgctg atatcaaaac gatcatccag cgcgccaatg aaaacaagca caccggcaca
    16201 agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat
    16261 ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac gtttgcggaa
    16321 gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag
    16381 tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca
    16441 tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa
    16501 agtccgttcc gaacccacca gccctgtgca aaaatcaacc atttgatatg caaattgcat
    16561 aacatgccag caaattccag gaattttcct aacatcgcca gcgcgacatt aagattacag
    16621 atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca aatttatgag
    16681 cgtttcatca tttcgaagaa atcgtcattg gtcttggtca tggccagttt attaatgagg
    16741 aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat ccacattttc
    16801 tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg gttgtagtcg
    16861 atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc catgttgcct
    16921 gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc gataagcgcc
    16981 gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc aaagaagcgt
    17041 ttcggacgat gaagggcatt ggcgtccaca ccaccggtca acactttacc tgacgccgga
    17101 acaacagtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat gataacgtct
    17161 ttcttgtgct caaccaggcg tttggccttc tcgatcacca tttccgcaac ctgaacgtgg
    17221 cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag acgctgcatc
    17281 tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca atccgggtgg
    17341 ttgtaagcaa tgctctgagc aatgttctgc agcagcatag ttttaccggc tttcggcggt
    17401 gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag tacgcgagca
    17461 gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt tgcgtgcagc
    17521 ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc gaagttaact
    17581 tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg aatcttacca
    17641 gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga aacgtagatg
    17701 tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc
    17761 aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg ctgcttcagg
    17821 atggcaaaaa taatgtcctg cttacgcatg cgagccaggt tttccagccc catattttcg
    17881 ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt catagtggtg
    17941 tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat ggcaggatca
    18001 tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac aggagagtac
    18061 gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc acttcgcatt
    18121 taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg tatgtttaat
    18181 acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta gcgacggggc
    18241 acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc aactgacctt
    18301 tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg
    18361 ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg
    18421 caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt
    18481 tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta
    18541 catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata tataactcca
    18601 caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg
    18661 actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc taccacacta
    18721 tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat ccgaaggttg
    18781 tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca ctggcccttc
    18841 cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc gggaaaacga
    18901 tggcgtttct tacgtcaacg tttcattatc ttctctctca tcctgcgatt gccgatcgca
    18961 aggtgaatca accgcgtgcc ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc
    19021 acgccgacgc ggaaccgctg gcggaagcta ccggcctgaa gctgggcctg gcttacggtg
    19081 gtgatggcta tgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca
    19141 ccacgggtcg tttaattgac tacgccaagc agaaccacat taacctcggt gccattcagg
    19201 tagtggtact ggacgaagcc gatcgcatgt acgatctagg ctttattaaa gatatccgct
    19261 ggctgttccg ccgtatgccg cctgcaaatc agcgcctcaa catgctgttc tccgccacgc
    19321 tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa tatattgaag
    19381 tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac ccttctaacg
    19441 aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac agagcgatta
    19501 ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg gcagatggtc
    19561 atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg
    19621 aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg cgtggtttgc
    19681 atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt gaagattacg
    19741 ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt
    19801 gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac tcaattccgg
    19861 taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc
    19921 gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag
    19981 gttaaataaa atgtcatact caaaatcatt caaactgtat caagatggca agagaatgaa
    20041 tcccgatgag cttactgtag taagtgattc gggtgaaaga atgcagccaa cacagagaca
    20101 gattgaagga tgaagagtat gggttccacc tcgtcgctgt atgcagccat tgatctcggt
    20161 tcgaatagtt ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg
    20221 cgaattaaac gcaaagtgcg tctggctgct ggcctgaata gcgaaaatga cctctctaat
    20281 gaagcaatgg agcgcggttg gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc
    20341 cctccctcgc aaattcgcgt tgtcgctacg gcgacattac gacttgccgt caatgcgggt
    20401 gattttattg ccaaagcgca ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa
    20461 gaggaagcgc gtctgattta tcagggcgtt gctcacacca ctggtggtgc cgatcagcgc
    20521 ctggtggtgg atataggcgg tgccagtact gaactggtaa ccggcacggg tgcacaaacc
    20581 acctcgttgt tcagcctgtc gatgggctgc gtcacctggc tggaacgcta ttttgccgat
    20641 cgtaatctgg ggcaagaaaa ttttgatgct gcagaaaaag cggcacgcga agtgttacgt
    20701 ccggttgccg atgaattacg gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc
    20761 gtgcaggcgt tacaggaaat catgatggca caggggatgg atgaacgcat taccctggaa
    20821 aagttgcagc aattgaaaca gcgagccatt cattgcggtc ggctggagga actggagatt
    20881 gacgggctga cgctggaacg tgcattagtg ttcccgagtg gtctggcgat cctgatcgcc
    20941 atttttaccg aactgaatat tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc
    21001 ctggtctacg gcatgttgca tctggccgtc gagcaggata ttcgcagccg tacactgcgt
    21061 aatattcaac gccgctttat gatcgatatt gatcaggcac agcgcgtagc caaagttgcg
    21121 gctaacttct tcgatcaggt ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg
    21181 ctcatcagcg cctgtcagct tcatgaaatc ggcctgagcg ttgacttcaa acaagctccg
    21241 caacacgctg cttatctggt gcgtaacctg gatcttcccg gttttacccc cgcacagaag
    21301 aaactgctgg cgacgctact gctcaaccag actaacccgg tcgatctctc atcgctgcat
    21361 cagcaaaatg ccgtaccacc gcgcgtcgca gaacaactct gccgtttact ccgcctggct
    21421 atcatttttg ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt acaggctaac
    21481 catgaactgt tgaccttgac gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa
    21541 gagattattg ctcaggaaaa ccagtggcag agctatgtcc actggccgct ggaagtgcat
    21601 taatgagtaa gtgccggatg cgatgctgac gcatcttctc cggccttgat tatttccctc
    21661 gttttgccgc catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt
    21721 cttcggcact gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca
    21781 gcaaaaagcg gctcggctcc gggcgcacca gttcgccgta ctgacggcgt tctttacaca
    21841 gcgtaaaggt caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct
    21901 catcgatatt atcttcatcg atactgctct ggtgcggcaa aaacccttct tccataccga
    21961 ccatgtagac ataaggaaac tccagccctt ttgacgcgtg gagagtcatc agttgcacct
    22021 gatccagctc ttcttcactc tcaccacgct ccatcatgtc gcgtaaagta aagcgcgtca
    22081 ccacctgggt gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc
    22141 agctaaacag ttggttgacg ttcttcatgc gcatttcggc agctttcgtg ctgggcgatg
    22201 tttcgtacag ccaggattca taatccatgc cgtggatcag atcgcgcacc gcggcaatcg
    22261 gctcccgctc cgccagacgc tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt
    22321 cataaccacg tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc
    22381 ttttattgcg cgtcatcgcc cactcaccca gctttttcag cgtcgccggg ccaatctctc
    22441 gcttcggcgt gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc
    22501 gcagataagc cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccagaaa
    22561 ttttgtacgg gatgcggttt tgcatcagga atttttcaaa cacccgcgac tgatagttac
    22621 cgcgataaag aatagcgtaa tctttgtact gcgttttatt gacgaagtga tgcgcaatca
    22681 gttcaccagt gacgcgctcg gcttcatgtt cttcgttatt cgcgcttaaa acttttagct
    22741 ctgtgccgta acccagttcg gagaacagac gcttttcaaa gacgtgcggg ttattggcga
    22801 tcaggatgtt cgccgctttc aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa
    22861 tcaccttcag cgccggaaaa tcctggctca gcagcaccag gttttgcgga cgtgcgccgc
    22921 gccaggagta gatcgactgg tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca
    22981 ccagcagttt caccagttca tactggctgg tgttggtgtc ctgatattcg tccaccagca
    23041 ggtagcgaat tttgttctgc cagcgctcgc ggacttcttc attgcgttgc agaagtagcg
    23101 tcggcaataa aatcagatca tcgaagtcga gcacattaca ggctttcagg tgtgcatcat
    23161 acagcccata acaatgggca aaaatacggt cccgctcgcc aatcgcactg gctgccgcct
    23221 gggacggtgt tttgagatcg ttcttccagt tagagatggt cgaaatcaac tgttgcagga
    23281 gaactttgtc atcttcaatc agcccttcgg tcaactcttt gagcaacgcc agctgatcgg
    23341 tatcgtcaaa cagcgaaaag ttcgccttca tccccagcgc cgcatactca cgcttgatga
    23401 tatccagccc cagcgtgtgg aaggttgaga tcatcagccc atgcgcctct ttgcgaccca
    23461 gcgtctgtcc gacacgctct ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg
    23521 cgatatgtcg cgcctgataa ccgcacccgc ggatcagatg ggcgatttta ttggtgataa
    23581 cacgagtttt accggaaccc gcacccgcca gcaccaggca ggggccggta acgaattcga
    23641 cagcttgttg ttggccgggg tttagacgca taggaaaatc gctcaatgaa agtcgggagg
    23701 ggaaaataac agcgtggtag tatagccagc ctcatcctta ccactcaagg cacgatcatg
    23761 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    23821 ctcgtgcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc
    23881 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    23941 ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct gcacacccag
    24001 ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct tctccgggag
    24061 aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca tatccgtcat
    24121 atagccgcgc agtttcttac ctacttgctc aatcgcatgg ctgcgaatcg cttcatttac
    24181 atcacgcagc tgcgcgttat ctaccgcacc ttccggaata gctttaccca aatcgcccgg
    24241 ttgcagttct gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt
    24301 accgtactcg gcagtatcag agataaccac gttcatttca tacagacgct tacgggcgat
    24361 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    24421 gccggaatca accatggttt cgaacgccag ttcaacgccc gctttcacca tcgcgatcat
    24481 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    24541 ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    24601 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    24661 gaacagcggt gccatgatct ctttcagctg ttcagacaac gcgtaagcac gcagtttcgc
    24721 cgggttagag agacggtcca tcatcagtgt gataccgccc tgcttcagcg cttcggtgat
    24781 ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac
    24841 cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga tggtttgctc
    24901 acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
    24961 accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
    25021 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgcac
    25081 ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac ggatctgctc
    25141 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
    25201 tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    25261 atccgcctgt ggaatcagtt cttcgtaagt acccacttta aaaccatttt cggtcgcttt
    25321 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    25381 accagaatca cgcatgttca gaccctggtt cagaccttgt gcgccacagc cgacgatgac
    25441 tactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg
    25501 acatttgccc agctgtgcca gctgctgacg cagattcagt gtattgaagt agttagccat
    25561 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
    25621 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
    25681 ctgcaacgtc aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
    25741 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca
    25801 cgctctcacg gcagattcag cgcctggaag aagatctcgg ccagccgctg tttgtgcgcg
    25861 ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa
    25921 cgctgttgca gtatcagcag ctgcgccaca ccatcgacca gcaagggccg ttgctctatg
    25981 gcgaattaca tatcttctgc tcggtgaccg ccgcttacag ccatctgccg ccgattctgg
    26041 atcgcttccg cgcggaacac ccgtcggtgg agatcaaact cactaccggt gatgcggcag
    26101 atgcaatgga aaaagtcgtc acaggtgaag ctgatctggc gattgccggg aagccagaga
    26161 ctctgcccgg cgcggtggcg ttttcgatgt tggagaatct ggcagtggtg ttgattgcac
    26221 ccgcattgcc ctgcccggtg cgtaatcagg tttctgtcga aaagccggac tggtcaacgg
    26281 taccgtttat catggccgat caggggccgg tgcgccgccg cattgagttg tggttccgac
    26341 gcaataaaat cagtaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    26401 cgatggtggc gctcggctgt ggcgtggcat tgttgccgga agtggtgctg gaaaacagcc
    26461 ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgagaaa acgccgtttg
    26521 agctgggcgt gtgtgcgcag aaaaagcggc tacatgagcc gctaattgag gcattctgga
    26581 agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    26641 cgtggcaatc gtagcccagc tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    26701 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    26761 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    26821 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    26881 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    26941 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    27001 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    27061 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    27121 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    27181 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    27241 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcacgca
    27301 catcttcgaa taaatccttc atcgccgcac agatcgcatc gctatcgacg gtgatgatgt
    27361 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgcgacac
    27421 cttcggcgaa cagtcccacg cgtggcagat caaccggatg acccgcatcc agcgctgctt
    27481 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgcggc atcagttgtt
    27541 tgatcagcac cgctacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    27601 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    27661 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    27721 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    27781 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    27841 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    27901 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    27961 agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca agacgcgacg
    28021 acagtttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca
    28081 gcactgctct taaatattcg gcaccttccg gagcaccgga caggggttgc gagtcagcca
    28141 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    28201 gctggcgtag gcgcacaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    28261 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    28321 taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt caatcagacc
    28381 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    28441 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    28501 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    28561 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    28621 ctggctttcg tacactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    28681 ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca ggccgccgtc
    28741 tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt catcgagaga
    28801 atcccaacgg caatcctgcg agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg
    28861 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagagtttg
    28921 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    28981 ttcaccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttttg
    29041 ggtgctcggc gcaactttgc ataactgcgg gactttacgc gaaagtttgt cgatatcact
    29101 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt
    29161 agagccgccc atcgcgatat ccagcgtcat ggcattttca aacgccgctt tgctagcaat
    29221 attgcgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    29281 gcgtttaccg gcattgagga acaactgctt acggtccgcg tgggttgcca gcagcgagcc
    29341 gttgcccggc tgcgacaggc ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    29401 catcccggag caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga
    29461 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gtttgattat
    29521 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    29581 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    29641 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    29701 ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    29761 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    29821 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    29881 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    29941 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    30001 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    30061 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    30121 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca atctgaatac
    30181 cgtctacgct gcgcactggc gtgatttctg ccgccgtacc ggacataaag acttcatccg
    30241 ccaagtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    30301 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    30361 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    30421 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    30481 gcagggaaga gagatagtta ccaccggctt ttgccgcagt tgggatggtg tttggtgctg
    30541 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    30601 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    30661 ctcccatgcc aacattaccg acgaagatca gcggacggat ataggcgctg gtgagattgt
    30721 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgt gaaaccggga
    30781 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    30841 ccggtccttt gtgtgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca
    30901 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    30961 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    31021 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    31081 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    31141 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    31201 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    31261 tgttgcatca tgataatttc tccaacattt ctgaattact ggcacctggc ggcaccagcg
    31321 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    31381 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    31441 cgaaggcgct ggctaacatg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    31501 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    31561 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    31621 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    31681 caactgccgc cggtaaacca aaacccatgg tacctaagcc gctggaggtg atgaaatttt
    31741 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    31801 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggagcgtaga
    31861 tagcgtcacc gggatggtcg taacgccagg aatgttcatc acgcagctgc gcgcagtgtt
    31921 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    31981 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    32041 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    32101 gtgcacccac ggcgatcagc aggtcacact cctgcaccgc gaagtttgcc gctttggttc
    32161 cgtgcatccc cagcatgccc agatagtacg gataatctgc ttctactgcg cccagccctt
    32221 tcagcgtaca ggtggcaggc atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg
    32281 cctgcgccat ccccacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca
    32341 tctggcgcgc ttgctcgact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    32401 accacggttc cagatcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    32461 gaccaggacg acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    32521 ccagagactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    32581 cttcctgaaa tgcgtcggtg ccgataaacg gtgcggacac ctgaccagtg atggcaacga
    32641 cagggatgga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    32701 cagacgtggc gatacatacg ccagttttac cggtagcacg ggcataaccg atagccgcca
    32761 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    32821 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    32881 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    32941 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    33001 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    33061 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    33121 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    33181 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg ggcctatttt
    33241 tatgatggct ttaataattt caatagaaat tgttgttttt taaaaagaaa atagtgagtg
    33301 aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc tttgtccgct
    33361 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
    33421 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
    33481 aatggtgact caaggagggt ttatgtcact gtcaattgtt catacccgcg cagccctgga
    33541 agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac ccggcttaac
    33601 gatggtgggc ttaacagaaa caacggtgaa agaagctcgc gatcgcgtgc gcagcgccat
    33661 tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg ctccagccga
    33721 tctgccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc
    33781 agaacagctt acagccaata agttagatga atatgaatta gtcggagaac tagcgcttac
    33841 aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg
    33901 cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg
    33961 atgcctgata gccgatcatc tgcaggctgt ctgtgcgttt ctggaaggta agaacactct
    34021 cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt
    34081 tgtcggtcag gagcaaggaa aacgaggact ggaaattacc gccgctggtg ggcacaacct
    34141 tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct
    34201 gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa
    34261 tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc
    34321 gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc
    34381 gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc
    34441 cttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac
    34501 ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca
    34561 gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg
    34621 gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc ccggcatttt
    34681 gagtaaaacg gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg taatggccgc
    34741 cagagagcgc caatttaagc ggcagaataa actgaatgcc tggctggata gtccggaaat
    34801 acgccaattc tgcaaacttg agagcgaaga tgcgatgtgg ttggaagaaa cgctgatcca
    34861 tctggggtta tcgattcgtg cctggcagcg gttactgaaa gttgcacgaa ccattgctga
    34921 tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc
    34981 gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc
    35041 ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc
    35101 cggaaagggt gtgaaaacgt tttggacgct taatacgcgt catatacttg gaccacacgc
    35161 gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt
    35221 cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct
    35281 caagcagttg tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat
    35341 gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta
    35401 attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac
    35461 aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt
    35521 taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct
    35581 atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga
    35641 accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc
    35701 cttatgcaga aacgctcatg agcacctggc aggccgcccg caaggaggtg gcgcatacct
    35761 cacgacataa cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc
    35821 agtggttggg acgcttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc
    35881 gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta
    35941 ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg tatttcactt
    36001 tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactgg
    36061 agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac gaagttccca
    36121 ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca
    36181 cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca
    36241 caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatgcgttga
    36301 ttcgcgatct tttgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa
    36361 ggatgccggt agaaggattt acttcggaga ggattatttc agataaaaaa aatccttagc
    36421 tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt
    36481 ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga
    36541 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
    36601 accgaattct tttacaacca ccggttttat gaccggctta c
//
LOCUS       DAJTNW010000032        35188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNW010000032 DAJTNW010000000
VERSION     DAJTNW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(52..834)
                     /gene="istB"
                     /locus_tag="OO179_001698"
     CDS             complement(52..834)
                     /gene="istB"
                     /locus_tag="OO179_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5531797.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(831..1853)
                     /gene="istA"
                     /locus_tag="OO179_001699"
     CDS             complement(831..1853)
                     /gene="istA"
                     /locus_tag="OO179_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5531798.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIYDS
                     FCRGVA"
     gene            2469..2606
                     /locus_tag="OO179_001700"
     CDS             2469..2606
                     /locus_tag="OO179_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531799.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            3206..3985
                     /gene="ipaJ"
                     /locus_tag="OO179_001701"
     CDS             3206..3985
                     /gene="ipaJ"
                     /locus_tag="OO179_001701"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5531800.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(4932..5861)
                     /gene="virB"
                     /locus_tag="OO179_001702"
     CDS             complement(4932..5861)
                     /gene="virB"
                     /locus_tag="OO179_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5531801.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(6078..6314)
                     /locus_tag="OO179_001703"
     CDS             complement(6078..6314)
                     /locus_tag="OO179_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5531802.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(6318..8219)
                     /gene="ipaA"
                     /locus_tag="OO179_001704"
     CDS             complement(6318..8219)
                     /gene="ipaA"
                     /locus_tag="OO179_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5531803.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(8228..9226)
                     /gene="ipaD"
                     /locus_tag="OO179_001705"
     CDS             complement(8228..9226)
                     /gene="ipaD"
                     /locus_tag="OO179_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5531804.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSKN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(9277..10368)
                     /gene="ipaC"
                     /locus_tag="OO179_001706"
     CDS             complement(9277..10368)
                     /gene="ipaC"
                     /locus_tag="OO179_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5531805.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(10388..12130)
                     /gene="ipaB"
                     /locus_tag="OO179_001707"
     CDS             complement(10388..12130)
                     /gene="ipaB"
                     /locus_tag="OO179_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5531806.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(12136..12603)
                     /gene="ipgC"
                     /locus_tag="OO179_001708"
     CDS             complement(12136..12603)
                     /gene="ipgC"
                     /locus_tag="OO179_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5531807.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(12660..13286)
                     /gene="ipgB1"
                     /locus_tag="OO179_001709"
     CDS             complement(12660..13286)
                     /gene="ipgB1"
                     /locus_tag="OO179_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5531808.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(13302..13691)
                     /gene="ipgA"
                     /locus_tag="OO179_001710"
     CDS             complement(13302..13691)
                     /gene="ipgA"
                     /locus_tag="OO179_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5531809.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(13704..15188)
                     /gene="icsB"
                     /locus_tag="OO179_001711"
     CDS             complement(13704..15188)
                     /gene="icsB"
                     /locus_tag="OO179_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5531810.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            15502..17118
                     /locus_tag="OO179_001712"
     CDS             15502..17118
                     /locus_tag="OO179_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5531811.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            17128..17490
                     /gene="ipgE"
                     /locus_tag="OO179_001713"
     CDS             17128..17490
                     /gene="ipgE"
                     /locus_tag="OO179_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5531812.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            17490..17948
                     /gene="ipgF"
                     /locus_tag="OO179_001714"
     CDS             17490..17948
                     /gene="ipgF"
                     /locus_tag="OO179_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5531813.1"
                     /translation="MSRFVFILLCFTPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            17957..19072
                     /gene="mxiG"
                     /locus_tag="OO179_001715"
     CDS             17957..19072
                     /gene="mxiG"
                     /locus_tag="OO179_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5531814.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            19080..19331
                     /gene="mxiH"
                     /locus_tag="OO179_001716"
     CDS             19080..19331
                     /gene="mxiH"
                     /locus_tag="OO179_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5531815.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            19344..19637
                     /gene="mxiI"
                     /locus_tag="OO179_001717"
     CDS             19344..19637
                     /gene="mxiI"
                     /locus_tag="OO179_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5531816.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            19643..20368
                     /gene="mxiJ"
                     /locus_tag="OO179_001718"
     CDS             19643..20368
                     /gene="mxiJ"
                     /locus_tag="OO179_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5531817.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            20365..20892
                     /locus_tag="OO179_001719"
     CDS             20365..20892
                     /locus_tag="OO179_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5531818.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            20834..21529
                     /gene="mxiN"
                     /locus_tag="OO179_001720"
     CDS             20834..21529
                     /gene="mxiN"
                     /locus_tag="OO179_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5531819.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            21522..21929
                     /gene="mxiM"
                     /locus_tag="OO179_001721"
     CDS             21522..21929
                     /gene="mxiM"
                     /locus_tag="OO179_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5531820.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            21913..22341
                     /gene="mxiM"
                     /locus_tag="OO179_001722"
     CDS             21913..22341
                     /gene="mxiM"
                     /locus_tag="OO179_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5531821.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            22471..23103
                     /gene="mxiE"
                     /locus_tag="OO179_001723"
     CDS             22471..23103
                     /gene="mxiE"
                     /locus_tag="OO179_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5531822.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            23090..24790
                     /gene="mxiD"
                     /locus_tag="OO179_001724"
     CDS             23090..24790
                     /gene="mxiD"
                     /locus_tag="OO179_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5531823.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            24809..25876
                     /gene="mxiC"
                     /locus_tag="OO179_001725"
     CDS             24809..25876
                     /gene="mxiC"
                     /locus_tag="OO179_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5531824.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            25889..27949
                     /gene="mxiA"
                     /locus_tag="OO179_001726"
                     /gene_synonym="virH"
     CDS             25889..27949
                     /gene="mxiA"
                     /locus_tag="OO179_001726"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5531825.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            27962..28363
                     /gene="spa15"
                     /locus_tag="OO179_001727"
                     /gene_synonym="spaK"
     CDS             27962..28363
                     /gene="spa15"
                     /locus_tag="OO179_001727"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5531826.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            28367..29659
                     /gene="spaL"
                     /locus_tag="OO179_001728"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             28367..29659
                     /gene="spaL"
                     /locus_tag="OO179_001728"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5531827.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            29752..30108
                     /gene="spa13"
                     /locus_tag="OO179_001729"
                     /gene_synonym="spaM"
     CDS             29752..30108
                     /gene="spa13"
                     /locus_tag="OO179_001729"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5531828.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            30077..30955
                     /gene="spa32"
                     /locus_tag="OO179_001730"
     CDS             30077..30955
                     /gene="spa32"
                     /locus_tag="OO179_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5531829.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGLFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            30949..31830
                     /gene="spa33"
                     /locus_tag="OO179_001731"
                     /gene_synonym="spaO"
     CDS             30949..31830
                     /gene="spa33"
                     /locus_tag="OO179_001731"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5531830.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            31835..32485
                     /gene="spa24"
                     /locus_tag="OO179_001732"
                     /gene_synonym="spaP"
     CDS             31835..32485
                     /gene="spa24"
                     /locus_tag="OO179_001732"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5531831.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            32500..32760
                     /gene="spaQ"
                     /locus_tag="OO179_001733"
     CDS             32500..32760
                     /gene="spaQ"
                     /locus_tag="OO179_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5531832.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            32760..33530
                     /gene="spa29"
                     /locus_tag="OO179_001734"
                     /gene_synonym="spaR"
     CDS             32760..33530
                     /gene="spa29"
                     /locus_tag="OO179_001734"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5531833.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            33537..34565
                     /gene="spa40"
                     /locus_tag="OO179_001735"
                     /gene_synonym="spaS"
     CDS             33537..34565
                     /gene="spa40"
                     /locus_tag="OO179_001735"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5531834.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            34578..34820
                     /locus_tag="OO179_001736"
     CDS             34578..34820
                     /locus_tag="OO179_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531835.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10814 a         5318 c         7153 g        11903 t
ORIGIN      
        1 aaaagtgatc cacttatatc tccaccaacg gcccaatatt gatccaccgt tttactcagg
       61 attagcttct gctataaccc cggcctttcg tttctgtctg agtcgatagc tttctccttt
      121 gatttgaacg acatgtgagt ggtgtaagat acggcccagc atcgctgagg tcagtgctgc
      181 atcaccggcg aacgtttgat cccactgccc gaacggtaga ttggatgtca ggatcattgc
      241 gctcttttcg taacgtttag cgatgacctg gaagaacagt tttgcttctt cctgactgaa
      301 cggcagatag cctatttcat caatgatgag caggcggggg gccattactc cacgctgaag
      361 cgtcgtttta taacggccct gacgttgtgc cgtagataac tgaagtaaca gatctgctgc
      421 tgttgtgaag cgaactttga tacctacacg gactgcttca tagcccatcg ctattgccag
      481 atgggttttc cccacacctg atggccccag taatacgata ttttcattac gttctatgaa
      541 gctgagtgag cgtaacgact ggagttgctt ctgcggtgct ccggtggcga atgtgaagtc
      601 atactcttcg aacgttttca ccgccgggaa ggctgccatt cgggtataca tcgcctgttt
      661 acgttgatga cgtgccagtt tttcttcatg aagcagatgc tccaggaagt ccatataact
      721 ccattcctgg tctactgcct gttgtgacag cgcaggcgct gcgcttataa ggctttccag
      781 ttgcaactgt ccggcgagcg ccatcagtcg ttgatgttgc agttccatca tcacgccact
      841 cctctgcaga atgagtcgta gatggagagt ggatgatgct ggggggggtt gtcgaagctc
      901 accagatttt caccaggatg cacgtcatac tcttttttct ccggaggcag tgccagcatg
      961 gactgctgct cttcgagcca gcgatcacag ggacgggcct ggattgtttc atgctttcgt
     1021 tggttagcga catcgtgcag ccagcgcaga ccgtggcggt tggctgtttc aacatcgaca
     1081 gtgctcccca tcgggcgcag gcgagtcatt agtgggatgt aaaaactgtt acgggtgtac
     1141 tgcaccatcc gttccacctt acctttagtc tgtgccctga aggggcgaca cagtcgggga
     1201 gagaagccca tctccttgcc gaactgccac agcgaaggat ggaaccggtg ctgaccggtc
     1261 tgatatgcgt cacgttgcag aaccacagtt ttcatattgt catacaacac ttcgcgcggc
     1321 acaccaccaa agaagcggaa cgcattacga tggcaggtct ccagcgtgtc ataacgcata
     1381 ttgtcagtga attcgatgta caacattcgg ctgtatccgg gaacagcaac gaacacgtga
     1441 agcggtgagc gaccattacg catagtgccc cagtcaacct gcatctgtcg tccgggttca
     1501 gtttcgaacc gaacggcagg ctcctgctcc tgaggaaccg agagagaacg aatgaatgcc
     1561 ctgagaatgg tcattccgcc acgatatccc tggtctctga tctcgcgagc gattaccgtt
     1621 gccgggattt tgtaaggatg agcatcggcg atgcgttgac gaatataatc ccggtattca
     1681 tccaggagtg aagcaacagc aggtcgcggc gtatattttg gcggctcaga ttttgcctgc
     1741 aaataacgtt taacggtatt gcgggagagc cccagttctc tggcaatcgt ccggctactc
     1801 attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac cataagctct
     1861 cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg tgtggatcac
     1921 tattgcaccg ttcgtgacac ttgttgacta cctgtgcggc atctggtatc aaggtggcgt
     1981 gaagccggtt caggttctca tgcggaacgt tggaacctat atccccccga taccaaaggc
     2041 gaaatgtaag tgacaaaatg caatgaacac gttctccgtt aactgaggag catggtgata
     2101 aataaaggta aatgcagaag gtgtagagtc tcgcagattt ctgacatcat agagcctaac
     2161 attagatgca taacgctaca gattgtagaa tctctacagg tgaaatatac actggttggc
     2221 gtttatgtcg caaaacatgt cattcaggtt catctcacca ataaggatat gagtgaagtg
     2281 gaggataagt gagtctgctg tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt
     2341 ctgcttgata tgggagctta tggaggctct ctgtacaagg ttagggagct aacaaagcag
     2401 ggataaaaag tccgcctgat gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa
     2461 atggtgcgat gaatgttcag gctatcttta tcttgatggt ggttcagctg tttggtaaag
     2521 aaatcgctgt aacaatagaa gaactgcagg cagtactggt cccacatcgt ttgctcagtc
     2581 aactggtgaa gttccgtagc gcgtaaatta atgctttgca tgaaacgtta ctggagtgga
     2641 tgaaagaata cctgcatata tcattactgc gttggattac cagtacaaca gattgaatga
     2701 actggacatg ttgatagacg gcattgaaaa gcagcttata agcatagtgc ggcagagtta
     2761 attggagagc acttcgcctt ctgagtatta agtaagcatg gtggtacctg cggatactat
     2821 ctattcactg tgctcggtcg gcagccttac tgaccaagga gttgggggag ttgataacaa
     2881 agttgaaaaa acagctctca accaaggtgg ttattgtaac aatagcaaat aagctggtgt
     2941 gacctgtatc acaataaccg attatggacg taaatacgac aaaaatatgt cagtatcagg
     3001 ctgtattaat tgctgatgct gatggacatt aactcttaat aaaagggtgg atgctgaaag
     3061 gttgttatgg tgacggacgt gatgacgacg gaaaagagta tgaggttttg gatttaaagt
     3121 tcgttcgggg aataaggacc cggtcagcta attctcatag tagttcgcat tgctggatgc
     3181 aatactgtgc tgcaattact cgtcgatgtc ggaacaacgg aagccttgca aacggggatg
     3241 tattcataca ggtgttatgt tatatggggt tttattgcag ggggctatac caagagaata
     3301 tatgatatct catcagactg atgtgagggt aaatgagaat agagtaaacg aacaaggttg
     3361 ttttttggcg aggaagcaga tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc
     3421 tgcaaaggaa ttaggggttg ataaaatacc acaatacaaa gggtctatgt ccgaaatgac
     3481 aaggaagagt tctcttgatt tggataatcg ttgtgagcgg gatttatact taataactag
     3541 tggtaattat aatccgagaa tacacaaaga taatatcgcg gatgccggtt attctatgcc
     3601 tgataagata gtcatggcta cgaggcttct tggattgaat gcttatgtcg ttgaagaaag
     3661 taacattttt tcgcaagtaa ttagttttat ttatcccgat gcaagggact tattaattgg
     3721 catgggatgt aatatagtac atcaacgaga tgtgttatct tctaaccaaa gagttttgga
     3781 agctgttgct gtatccttta ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc
     3841 tgatggaagc tatatggatc cagctgtagg agaaaattat tcttgttttt caactatgga
     3901 attaggtgca agaagaagta attctaactt tataggatat actaaaatag gtatttccat
     3961 agttataact aatgaggctt tgtaaattgt cgttttttat ctgtgaagga gcttgaatat
     4021 tttggggcgg ggggcataac aaatgatatt actgagtaca aatttagcta tattgggcgg
     4081 attagttgtg tgggaagacg aaatgtttct cttgaaataa gtgaactccc tcaaggaatg
     4141 attgtgtgct taaatattta agtgctggta agtttgagaa ggctgacata ttttgcagag
     4201 caaacagtga ttgacttgga tgatattttt attttagtca ctcgttgtaa ttggagtatg
     4261 ctttatttta tttgaaaata gatgtatttt atacccgcaa tttaaactgt aagccctatg
     4321 aggggtatgg atattataag ttactctttg actaagcata acttttgggg ttagaaaaat
     4381 aaatctcttg ttaataatta atataatttt aaaatcaaac ttgtttataa ttataaaata
     4441 atgtaagtcg gtcattttat gggaagatta ggagaataaa acagtaatta tatttgagaa
     4501 atctgctaat atattcttaa agcttttttg atactgtgaa cgaagtctgt taatggtggg
     4561 ttttttgttt atcaccatgt aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc
     4621 gctgttttta ttgaaacgat taaaaatacg ctgaatattt tcggccagtc actcatcttc
     4681 acataaaatt ttgaccatca tgccgcatcc cttagttgca caattcattt tcaatgatat
     4741 gaatattact aatttcacca gataattttc tcgtggtcag cagggtatga gttttcaccg
     4801 taattttaag gaaacacagt gcgtggggat tgaataacag atagccgacg gaattacccc
     4861 accggctgaa agtcaagttc aacgaagatt tttattatct gaattgggca gtttacatca
     4921 gtgttcgatg tttatgaaga cgatagatgg cgagaaatta tatcccgaat agcttcatct
     4981 atttctctct gaactgtttt gtttatttta gataatgtaa atattagagt tctgtttgtt
     5041 ttattctctt ttctttttat gtaagtccgt ttgtctttag aaataaataa cgagtcaact
     5101 tttagcgaag ggttttgctt tctgtttttg cttttcttta tgatgtttaa aatttctttt
     5161 ttatatatgt ccgggggcaa atttgtatca aggtctttta tttcttcctt taatattggt
     5221 agtgtagaac taagagattc attagccttt tcaagtcctt tataataatt gaataatatc
     5281 ttgtagtcat taaagttaag ttctgacgcg attggaaata gagatattat ttcctgtgga
     5341 acgcttgctg cctgaaaggc acgagtgact ttcgcgcgag acagattctc ttttttggct
     5401 atgtctttat aggacatccc tgatactttc agaaaattaa gaccaatacc aagttctcgg
     5461 atgctatgct cttttgctgt ttgtatatcg tttgctagtt ttctggcatc aagagtagat
     5521 atatattctt ttgaatatag aacttcaaga tctgctcctg catatattgc agatgctctt
     5581 ctacgagtgc catccagaat ttcaattcta ccatcaatct cccttcctat tacagggaag
     5641 aattgttgta gctttatagt ttttatgata tcggctaatg attcttctgt tagggattct
     5701 tgatcgcgta cattcgtttt ttggttgacg aaggttaaat cttggatcct tttatgagga
     5761 atcttggctt tgataaagga aactttatta ccagaatgga gtgtaaactc tttcttttgg
     5821 ccttccttta tacttaacaa gtcgttgcac aaatccacca tatcacaccc tgtttattca
     5881 tattgattta tattgacaaa agttaaatgc agtgtgagca agattgtatg taactgttga
     5941 tgatagtcaa cggtagtctt aaaaattact attttaatag ctggattgat gcaatttatc
     6001 tttattggat aaaagaataa tattcttgtg gagctctgat gtgagagctc catagatgaa
     6061 aaaatgctta tcaattacta tattgatttt gtactatttt tgactacaga aataagatct
     6121 ccaatagtaa taatgtgttc taatgtagat tcatctatac gtagattgaa ctcaaaacta
     6181 atcatatcca gcatttcaat cagagaaaga gaatcggcag aaagatcttc aataaattta
     6241 gtttcttcag atagtttgct gtacgctata ccatagcaaa aggccactat tgataatatt
     6301 ttttctttta tcatatttta atccttattg atattcttta atacttttga tagggaagtg
     6361 gttacatctt ttgctgcttt atatatatta ttgttttcag ctgtaatatc attgttattt
     6421 tttaaatcag atatatcatc tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat
     6481 accttgctga gggcgctaga tacttctttt gccttttcat agatcgcatg atttttatca
     6541 attgtatctg cgttggtttc tgttacgtag tttcttatat ttgcatctgt aacttttaat
     6601 ttgttgttta ttctatgttc tgttgtggat tttgtaatgt cctttgattt ttcctgtaac
     6661 tctcgttctt gtgttcctga tttagttcca atgagagata taagattact tagggagttt
     6721 gttacttttt ttgaagcttc aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt
     6781 gataaaaaat cagtctgaag tacttttgaa tcagtgtttg agttgaattt attgatttgc
     6841 gaagctactc tatgagaaag agaagcgcta cttttagttg tatctgatga gttatcatta
     6901 ctgcttgtca ctccttccgg gtggagaggg ggggtggttg aagagttctg tatgttcaga
     6961 ggcctgtgtc cccgagaaag agagttattt gcagatcttt cttgactact cgtagttaac
     7021 aggtctgaac ctattccatc cgaattatct ggagtatggt tgaaaacctt ttctaaagct
     7081 gaattatttg caaatgtctg gacagtatct gatatagagt gtggtagaac ggaagttact
     7141 ttttgaacta gtgctctttg tggatttagt aatgagtttc cgttacggga taatagatcg
     7201 tttgtctgag aagtaggaga ctgtgcatca ttttctgggt tctcgtgata gctattgtca
     7261 tatactctgt tgtcaaaaac tctattatcg taagttctat tatcattatt tatatgatag
     7321 tgaattacag gttgggatgt tttctcattg gctccggggg ttggtccgcc atctggcata
     7381 tcttcgggag gtgtagggag gccatcttgg ataggatgcg aatcttttat atcttctaaa
     7441 atatcattaa ggacttttct caacagctca ggaaacacgg ctactgtatt taagtctctt
     7501 gacagtttac ctataccaaa tcctttacta tcaatagtat ttttacgttg attgcgcaaa
     7561 ctatcaaagg caagttttgc ctgtgcgtca actaaatcat ctagtttgag agatgaaaca
     7621 gtttcattac ttaactttaa ttcagcaaga atattattct tgataaatga tgatacttca
     7681 gtattgagga gtttttgtcc caatgataaa gcttcaccag atgtgtgttg ttgtgctaat
     7741 agtgcaagtc tgtgctcaat gttttgtcgt aattgatgga gtttcttatt ctttgccgac
     7801 catggtccaa aggcagaatc ttgggataat ttatctttta taattagttc cgaatactgt
     7861 gcgacaattt tttgaagtcc cttttcgttt tcaaaattaa gaccattttg atgtactaag
     7921 gagtacaaaa catttgaaaa ttcgagaatc tcgcttttgg catacgcatt cattgagttg
     7981 gaataaatgc ttcttaacac atcttctttc cctgaggaag aaaataaaaa atcaagacat
     8041 ttctgattaa aagacgttgc aaagttttct ttttcagaca ctgatgctgg tgtttttaga
     8101 gaagtttgcg aactatggat atcggatatt ttgcttttta actcgctgta ttctgttgat
     8161 gatggtgaag ttgccttata taagaatgtt ggcgcttgag tattatttac attatgcatg
     8221 gcgcacctca gaaatggaga aaaagtttat ctgtatctgt acatgagctt attgtactac
     8281 tcaaaacctt tactaaatta tcaaaaatac tattggcatt actgtatttt tgaactaaag
     8341 tttgaagatt atttttcatt gtttcatctt cggcagagaa tccggcattc catgcctgat
     8401 attttgcatt atctagcaca acctcgccat ttccacctag attctctaag ctttttaaca
     8461 tattgtctat tggggtcatg tttatactga caacatatcc cccgtttttt tgagatacct
     8521 tgccgattgt tccacctaat tctgtaagcc atttatttgc ttgttcctga ctaacagtat
     8581 tatttgctgg atatagcggt ttatctttat atttttcctt gagttcttcc aatgcctttt
     8641 taagcgagtt gacttgtaat ttcacggagt ttccgtcgtt acctccggga gagatccagc
     8701 cggcaagact ggaaagaaca gcgctaaaat cttgatacat ttgagtatat gaactaacgg
     8761 catgttcata tactttcaga tactgttcat taatattatt gatggagttt gcaattttag
     8821 cccacagttc tctatgagat atcatttgat ctccatcaag ctcggcttct ttcggagctg
     8881 aatgtaataa ttctcttgcg tctttattaa ttggatattc gttcttggaa agaatattca
     8941 atagttgagc ggatttattt acatccatgc taatctgaga tgaatgtaat gctatttctt
     9001 ctagcgatgt tttagtcaac gttttttgtg aaagctcttt ctttaatgcc tgatttgttg
     9061 ttctgatatt atgaagggtg tcgttgagca tagtaaggga acttacagga tgagaactgg
     9121 tcgttgtttt tatatcagaa ttaactgttt cggttgatga accgttggta ttgtttggac
     9181 tgaatgatga ggtggaaata ctattagtca gagttgttat attcatggtt atttccttac
     9241 aggaagagcc atatattatt atggctcttg gatatattaa gctcgaatgt taccagcaat
     9301 ctgactggct gtcgaattct ttgattggtt gatgctgtca attatattca ataatttttg
     9361 tattaattga tttgtcgctt gggatgcttc tttagatact tgggatgctt cctctgcttg
     9421 tttactgctg gcctgactga ttagttgttc ttcttcttca agagcagatg cataacgccc
     9481 tcctgatgtg gatatattac cagcaacggc tgatgtttcc attgttgcac ggccaatgtt
     9541 ttgtttttgc tgcgcagaaa gggtattgag ctcgtaagtt cttctctggg tgtcaatttt
     9601 atcctgcaaa gaaatatcgg gagaactaag agaagtttta tgttcagttg acagggatat
     9661 attatctggc gccagtttat ttttacctaa aaattttgtg ctagagttag tttgtggtga
     9721 ggtgatattt gtatctattt gtttatttaa ccctaattta gaacctgcaa gctctttttc
     9781 aagagattga gcagtggcaa ggttctttct taaggctcct ttttggtcgc taatccctga
     9841 atgcgttttt ttggcaccga tacccgttat acctacttgt gtgactgctc cagtaatgct
     9901 tgatgatagg gctgccaggc cttgccgaac aatgttctct gcagctgatt ttgtagcatc
     9961 gaacgcaatc aatgacaatt gagagcccaa tttagtttct gcagtgcgga gagcagaaag
    10021 tagaacggct acactaataa ttaaagaaac agcattagaa gaaagactgg aaatatccag
    10081 agtgttctct ggagtaaggg tgtgactcat ctctgaaaga tctttcatct ttttgtcagt
    10141 aaggcgagca atgatttctg aatcatgctg aacctgctct gataacttta aaagttgatc
    10201 atcatttaat gtggttgtta atacgggatt tttaccgaca ttaagtggaa tatgcgctgc
    10261 aatctgctga taattttgtg atttttgtgt ttcggaagaa ctttgagttt gttttgtgga
    10321 tatatctgta tataaaatct gggttggttt tgtgttttga atttccataa cattctcctt
    10381 atttgtatca agcagtagtt tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct
    10441 gagagttgga cattgaggct aatagatctg caattacttc ctgcaattgg ccgaattttt
    10501 ctattgcttc actgatatat tttgacagtt gttcaacttg atatttcgat aatgtcaggt
    10561 ctgctagatt tgtcgacgcg ctgttctgga aaacagcaga agcgacactt cctcccgctt
    10621 gtgtggcaga gttaacactt tctcctaata aaactgcttg gtttaaatgg gtggaaataa
    10681 tttgtttgga tattacttca tcacccgctg caccaagaaa tttatttaat ctggcaacag
    10741 cattagtgat taaatcgtcc agttgagaag aaaaattctt gagaaacttt ggtataaggt
    10801 ctgtgagggt tttacctatt attttgccaa tattttctgc aagttttgct gctgcctgtt
    10861 taccaacagt ggctacgaga acgactgctg caactaggac aagagcgcct gcgattgccc
    10921 ccagaataga gccaatcatt ttggcttttt tcgagtcgac gcccaagcct tcgagcattt
    10981 ttgtaaatgc atctgaaagg agtttgatta agggttcaat gactgctttc atgatcggat
    11041 tcagggcttg ttccatgaag gaattgccgg tcgctgcttg tactatagca tccgtaacca
    11101 taagagctaa accaacagct gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa
    11161 caacactaac gatagttaaa agtgccccaa gtattttccc aacacaaccc attactctgt
    11221 tgagttcttc tgctttacgt acttcagcag catactcatc agattttctc tccatttcag
    11281 tttttcttga ttcttggaga gactggaata gagccagatc attttttaaa gattcttcat
    11341 tattttttcc aactagttga ataaaggttg ccatcaattg agtaacactg gcaagtccgg
    11401 ttaatgattt ctgctgggtt gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa
    11461 aagagtctat ttctttttcg agttgcattg atttatctgt aagtttgcta tgaattgaca
    11521 gggttttctg ctcaatcaat gtcctgtctt taactgctgc gtcttttttg atagtgagtt
    11581 gtatttcttc ccggcttaat tttttctttt ctggtgactc tgggtcgagt tcggataatc
    11641 ttgtttgaat ttggttaatt ttattttcta ggtcttttat tttagaatct gcgtttttta
    11701 gtttattaat ttgtttttca tagtctctgg ttagtccttc agtttcagat agaagagtgt
    11761 taattttatc ggagaattct aggttttttt gctgtcttgc ctgttgctgg gacttccaag
    11821 cagtaatttt atttgttaat gcagttaaag atttttcacc gagtatttga ataaggtttc
    11881 caattaaaag cgttagttgg gaacttgcat ttaatgactt tggtgcttta agttcaggga
    11941 ttaatatatt tgaagttgag tgtgcattag ttgtattaat attttggtta gcagtaagat
    12001 cagcaattgt aagagaaaat aatttgttag ctgcatcatt tgcagcttgg atagtattgt
    12061 ctccaagctc agtggaagcc aatattttgg caagaggaaa accagtggtt gtggtgctta
    12121 cattatgcat aataattact ccttgatatc ctgaattgcg tccaagtatg attgtgcttt
    12181 tatttttaat ttttcatcat tgctgtgttg aattacgagt tcgaagcact ctttagcttt
    12241 taagggggct ttcaaccgaa gctggcattg tccagtatgg aatactggtg tatagtcatt
    12301 ttttcctaat gcaaaagcga cagcataaag gtctgctgct tgttggaact gttcttttat
    12361 ctgataaata gctgcgagtc ccataatgta gtctacattg taaaagtcgt atatacataa
    12421 aaacctgaag aaaacttcag cttcctctat tcttcctttg ttgtaaaagt cataagcata
    12481 tgaataaatg tcatccatca tatcatcagg aattgcatta atatctttca gtgtagcgcc
    12541 agagttaatt gcatcaatta ctgcagtaga gatgctttca ttttcggtga tatttaaaga
    12601 cataaggtct cctttttgtc tgctttactt taataagtat aagatttaat ataaaagatt
    12661 taatttgtat tgctttgacg gtatacagcc tttagaacct ctgcgcttat ttctatttgt
    12721 aaaacttttg ttgattcatt aaatatttta tcgtagacgg aactggatac tagatttgta
    12781 agatccttca tttgtgtgtt aaacaaatcc atgtcactgc tatagcgttt taaaatcttt
    12841 gctctgaggc cagatgaggc aatctcgttt agtgctgtat gactgataga tgtttgtgct
    12901 gatttttcat caagttttat atccgggaat tttttttcaa tagctgcaaa tatgtttttt
    12961 ttgttatctg aggtcaaaac aactccattg tgtatggcca tttcatctat acttctgttt
    13021 atcattgaag agacataagt ggttcgctct tggctcatcc aacaaaatag ttgattcccc
    13081 gaattactat cttttatttt gtctatgacg ctgtccctga ttttatttat attcaccggg
    13141 catccaaaat tcttctgagg aaagcgttgt tttaagttaa aaacagataa tattttcttt
    13201 acaagcttat tgcgacttaa cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt
    13261 ggaagtattt tgtttagaat ttgcatgata ccccctatat gttagttcac ttctgaagtg
    13321 atgtttgcaa tggttagttt aatacaaaat aaaaattcat acaaagattc aatgagttca
    13381 tcaggtgaaa gatatttttt gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg
    13441 agaagacaga tagaagaacc ataatctcta ctcaaagagt agttaaggat gagtatgtgt
    13501 tctggattaa taatattttc tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag
    13561 atgtattcat cattatctgt tatacaaaca ggtatttgtt cttcgagcaa tataaaggct
    13621 tgatttttat cgttaaaatc aagctttgca cctgttattt cataaagttt atcatatagt
    13681 ttgcgacaca tttctatggc cttctatata ttagaatgag agttattcaa taaaagctta
    13741 ttgatctgta ataagtcgtg aatgtaaagt ttggttccaa gatctggcga tttaagagaa
    13801 ttgtaataat caataatttt tattgatttt tcgatgattt cttctttttt atctgttgct
    13861 tcggttttta attcgtttaa tacacgagtc atattgctgg gtgctgattt tactaagtat
    13921 gagtggcaag cgttgatgac cgcatcaaag tcttctgaag atacattttc agtaggatgt
    13981 ttgtaaagtg aaattgataa aggcttgaat gtttttggtg tgtggataag gttaatttta
    14041 ttgaaacttt taagatagct gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat
    14101 tcaatatact tcattatttc gttataagac tgattgagac tttcaatgcg ttgcctaact
    14161 ttatttgcat acgcgtatac atcgttaggt gtagcaacca atttaacgtc agggagtgga
    14221 aagtatattt cagaatttcc tgctttaaga acattaagtg ccataccagc acagttttca
    14281 gatttactga ttaatttata atttgccaat tgtctgaaag catctgcttt ctttttgcat
    14341 ataaattttg acatgtttgt ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc
    14401 tttgttttat tatcacctga taatggaatg tatactttat cagcactgac cccccaataa
    14461 ccataggggt cttttttttg attagctctg ggaaaaaata tttttttgtt gttattttga
    14521 tcttgttttg aatttaataa gtttctaacc tcttggcctg catcaagtct ttcggctgtt
    14581 tggtctgaaa tcatatttag tttgtcttgc ttataggtat cgacactgta acctgagcgt
    14641 ttttcaaaaa ggtagtttat ttctgtatct tttgtaacat tcccgccggg ataccagctg
    14701 agataggtat gagttggttc ctgtagtaaa ttgtttttta tactgatact agtatgccct
    14761 aaatgggtta tgcctctatt tccccagcaa cgaaaaacta aatgtctttc aagatttttg
    14821 gctgtaccga tgccatgaaa acgctctgcg gtattcaaca ttaatttgat gtctctacct
    14881 gtgaaagagt gttgaattga gtttttagct gcttcatagg cggcaaacgc actttctgca
    14941 gatttttcgc tagctaatgt attgaggaaa agcaataagg atttttgatt ctctagtcga
    15001 taatttttta gttgttcatt taatatctgt gaatttatct ttgcattaat agtgcttaat
    15061 gttctgaaaa agagatcctt taggttgaat ttctgtgcta tcaatatagg tccatgctct
    15121 gtatcttcaa cgcgtatagg cccttttgta ttgcttgcgt caatgaaatt gctaattttg
    15181 aggatcatac tttattaact ctccattact tggtgattgt ggggatgtaa aatgtatttg
    15241 gtatataata tattcaatta ataaatttag aaacttgagc ctgttaacat aatcaaattt
    15301 tcttttgctg tacataatat gtacctcgtg agcatatgta gtgctcgttt catcatgaaa
    15361 tcccacaaga taaagtgcct gatgtatcag gctcggagtg ttatagaaaa agagagaacc
    15421 ctgttgaata agtatctttt ttgttgtaaa acgaggtaca aaactgcaat caatgcccca
    15481 tttatgactg aggataatta aatgcacata actaatttgg gattgcatca ggtttcattt
    15541 caaagcggag attcctataa aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg
    15601 atttcatatc aaagagttaa aaatggagaa agaaataagg ggatcgaagc gcttaatcga
    15661 ttatatttac agaaccaaac aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga
    15721 gctgaagttt tttgcgaggc aataaagttg gcaggtggtg atacgtcaaa aattaaagcc
    15781 atgatggaac gattagatac ctataaactt ggtgaagtta ataaaagaca tattaatgag
    15841 cttaataaag taataagtga agagataaga gcacagctag gcattaaaaa taaaaaggaa
    15901 ttgcaaacga aaataaaaca aatatttact gattatttaa ataataagaa ttggggaccg
    15961 gtcgacaaga atattagtca tcatgggaaa aattatggtt ttcaattaac tcctgcctct
    16021 catatgaaga tagggaataa aaatatattt gtcaaagagt ataatggaaa aggaatttgt
    16081 tgtgcttcta cgagagagag tgatcatatc gcgaatatgt ggctttcaaa agtggtggat
    16141 gacgagggga aggaaatttt ttcgggtatc agacatggag ttatatcagc ctatggattg
    16201 aaaaaaaact catctgaaag agctgttgct gctcgtaata aagctgagga gttagtaagc
    16261 gcagcattat atagcagacc agagttatta tcacaggctt tgtctggtaa aacagtagat
    16321 ttaaagattg tttcgacttc tcttctgacg ccgaccagtt taaccggggg agaggagagt
    16381 atgttaaaag atcaggtgaa cgcattaaaa gggttaaact ctaaaagggg ggagccaacg
    16441 aaattattaa ttcggaatag tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg
    16501 gtgacattta actttggtgt aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat
    16561 gttgacaagc ttaatgatga atcaatatgt tctttgttgg gggataattt cctcaaaaat
    16621 ggtgtgattg gaggttgggc tgcagaagca atagaaaaaa atccaccatg taagaatgat
    16681 gtgatatatc tggctaacca gataaaagag attgtaacca aaaagttaca aaaaaatgat
    16741 aatggagagc cgtataaatt gtcacaaaga atgactctat tggcttatac tataggtgct
    16801 gtaccttgct ggaattgtaa gagtgggaag gacagaacag gtatgcagga tgctgaaatt
    16861 aaaagagaaa taataagaaa acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta
    16921 tcctcagaag agaaaagatt attttctact attctaatga atagtggtaa tatggaaatc
    16981 caagagatga atactggtgt gcccggaaat aaagtcatga aaaaattgcc gctatcttcg
    17041 ttagagctat cttattctga aagaataggg gacccaaaaa tatggaatat ggtgaaaggg
    17101 tattcgtcat ttgtataaga ggaatatatg gaagatttag cagatgttat ttgccgagcc
    17161 ttgggtatcc ctttaattga tatcgatgat caagcaatca tgcttgatga tgatgtgcta
    17221 atatatattg aaaaagaagg agattcaata aatttattat gccctttttg tgcattgcct
    17281 gaaaatataa atgatcttat atatgcgttg agcctaaatt actcagaaaa gatatgctta
    17341 gctactgatg atgaaggcgg aaatctaatt gcgcgattag atcttaccgg gataaatgaa
    17401 ttcgaggatg tatatgttaa tacagaatat tatatttcgc gtgtgcgttg gctgaaggat
    17461 gaatttgcgc gaagaatgaa ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt
    17521 ttaccccata tttagggaga gctgattgtt gggataaggc tggtgaaagg tacaatattc
    17581 catcaagttt gttaaaagcg attgcggaaa aagaatccgg atttaataaa tctgcagtca
    17641 atgttaataa caatggaagt aaagattatg gtataatgca aataaatgac tttcattcta
    17701 aaagacttag agaaatggga tattctgagg aaatgctaat tagtcatcca tgtctttctg
    17761 tacattatgc agcaaagtta ttaaatgagt ttatgatgat gtatggaaga gggtgggagg
    17821 ctgttggagc ctataatgca ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg
    17881 cagaggatat ctatagacgc tatttgcgga tagcagcaga gagcaagcag aataatcgaa
    17941 ggatataaga ggatttatgt ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt
    18001 agtgaaactc acaaacggtg tcggggatga gtttccgtta tattatggga ataacttaat
    18061 tgttctcgga cggacgatag aaacacttga gtttggaaac gacaatttcc cagaaaacat
    18121 tattcctgta acagatagta aatcggatgg tataatttat ttgaccataa gcaaagataa
    18181 tatctgtcaa tttagtgatg aaaaaggtga gcaaattgat atcaattcac aatttaattc
    18241 atttgaatat gacgggattt cttttcacct taaaaatatg agggaggata aatcgagagg
    18301 ccatatacta aacggaatgt ataagaacca ctcggtgttt tttttctttg ctgttattgt
    18361 tgtgttaatt ataatttttt ccctctcttt aaagaaagat gaagtaaaag aaattgctga
    18421 aattatagat gataagagat atggaatagt caacactgga cagtgcaatt atattcttgc
    18481 cgaaacacaa aatgatgcgg tatgggcgtc tgtagcctta aataaaaccg gatttaccaa
    18541 atgcagatat attctggtgt caaacaaaga aataaataga attcaacaat atatcaatca
    18601 acgattccca tttatcaatt tgtatgtgtt gaatttagtg agtgataaag cggaattact
    18661 ggtgttttta agtaaagaaa gaaatagttc aaaagatacc gaacttgata aattgaaaaa
    18721 tgctctgatt gttgaatttc cgtatatcaa aaatattaaa ttcaactatc ttagcgatca
    18781 taatgcaagg ggggatgcta aaggtatttt cacaaaggta aatgtccagt ataaagagat
    18841 ttgtgaaaat aataaagtta catattctgt cagagaagaa ctgacagatg aaaagcttga
    18901 gctcattaat agactgattt ctgaacataa aaatatatat ggagatcagt atattgagtt
    18961 ttctgtactt ttgatagatg atgattttaa aggtaaatca tatcttaaca gcaaagacag
    19021 ttatgtaatg ttgaatgata aacactggtt ttttttagat aaaaataagt gaggataaaa
    19081 tgagtgttac agtaccgaat gatgattgga cattgagttc attatctgaa acttttgatg
    19141 atggaactca aacattacaa ggtgaactaa cattggcact agataaatta gctaaaaatc
    19201 cttcgaatcc acagttgctg gctgaatacc aaagtaaatt atctgaatat acattatata
    19261 ggaacgcgca atccaataca gtgaaagtga ttaaggatgt tgatgctgca attattcaaa
    19321 acttcagata atagggagca ttcatgaatt acatttatcc agtcaatcag gttgatatta
    19381 tcaaagccag tgattttcaa tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta
    19441 aatatagtga tattaagatg gatacagata ttcaagtatc acaaataatg gagatggtaa
    19501 gcaatccaga atcattaaac ccagaatctt tggccaagtt acagacgacg ctctcaaatt
    19561 attcaatagg agtatcatta gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa
    19621 ctttattaaa gtcttaattt atatgattag gtataaaggt tttattttat tcttgttgct
    19681 gatgttgatt ggatgtgagc aacgtgaaga gttaatttct aatttatctc aaagacaggc
    19741 aaatgaaata atatctgtgc tagaacgcca taatattact gctagaaaag ttgatggagg
    19801 taaacagggg atctcggtac aagtcgaaaa ggggacattt gcatcggcag ttgatttgat
    19861 gcgcatgtac gatttgccaa atccggagag agttgatatc tcacaaatgt ttcctacaga
    19921 ttcattagtg tcttctccaa gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg
    19981 gctggaacag tctttagttt ctattggtgg tgttatttcg gcaaaaatac atgttagcta
    20041 tgatcttgaa gaaaaaaata tatcttcaaa accgatgcat atatcagtaa tcgctatata
    20101 tgactcaccg aaagagtctg aactattagt tagtaatatt aagcgatttt tgaaaaacac
    20161 cttttctgat gttaagtatg aaaatatatc tgtcatatta actccgaaag aagaatatgt
    20221 ttatacaaat gtacaacctg ttaaggaagt taaatcggaa tttttaacaa atgaagtaat
    20281 atatttattt ctcgggatgg ctgtactagt tgtcattctt ttggtatggg cattcaaaac
    20341 agggtggttc aagagaaaca aaatatgata agaatggatg gaatttataa aaaatatctt
    20401 tcaataattt ttgatccagc gttctatata aatagaaatc ggttgaattt gccttctgaa
    20461 ctgttagaaa atggcgtaat cagaagtgag attaataatc tcataattaa taaatatgat
    20521 ctaaattgcg atattgaacc tttaagcggg gtaaccgcta tgtttgttgc caactggaat
    20581 ttacttccag ctgttgctta ttttatcggt agtcaagagt ctcggttaat taatcatagt
    20641 gagatggtta ttagttatta tgggggcaaa atcagtaagc aaggggaggc cgctattcga
    20701 agtggttttt ggcatcttat tgcgtggaag gaaaatatat ctgttggtat atatgaaaga
    20761 ataaacttat tatttaatcc aattgctttg gagggaaatt atacgcctgt agaaagaaat
    20821 ttgtcccgat taaatgaagg tatgcaatat gcaaaaaggc actttaccgg tatccagaca
    20881 tcatgcctat gatggtgttg ttataaagag aattgaaaag gaattatgta aaaccataaa
    20941 agatagagac actgagagta agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa
    21001 agctgaatca ctgcgtatag atgctgtatg tgatggttat caaattggta tacagactgc
    21061 ttttgagcat attattgact atatttgtga atggaagtta aagcaaaatg aaaacagaag
    21121 aaatattgag gactatataa ctagtttgct ttcagaaaat ttacatgatg agaggatcat
    21181 tagcacttta ttggagcagt ggcttagttc gttgcgtaat acagtaacag aattaaaagt
    21241 ggttttgccc aagtgtaatt tagcactgag gaaaaagtta gaacttgatc tccataaata
    21301 tcgctcagat gtcaagatta ttttaaaata ttctgaaggg aataattata tattctgtag
    21361 tggtaatcaa gtagtggagt ttagtcctca ggatgtaata tcaggtgtta aaatagaact
    21421 tgcggaaaaa ttaacaaaaa atgataaaaa atatttcaaa gagttagcac ataagaaact
    21481 gagacaaatt gcagaagact tgttaaaaga gaatcctgtt aatgattaat caaataaatg
    21541 caagcaatgc tctgcagcaa agattaaata gtgaagaatt tgttaattta aatgagagat
    21601 tatcatcttc gcaatcattt gatgaggata taatctatga aataatgcaa tatttctcac
    21661 agagtgaatt aaattccata gataatgatg aactgcacaa taagattgaa caattattca
    21721 attcacgttt tccttatctt acagctgcac aaaaaagttc acttcttaat aaactcatag
    21781 atgcaaatca atatgtcgat ttgcatgagg gattttatgc atcattgtcc atatataaca
    21841 atattgattt ctatattaag actacaacct tcgattcatt aattagtgtc tttgaagcag
    21901 ggagagaggc agatgattcg acatggtagt aataagttga aaatatttat tttaagtata
    21961 ttgctattaa cactgagtgg gtgtgcttta aagtcatcat ctaattctga aaaagaatgg
    22021 catattgttc ctgtaagtaa ggattatttt tctattccaa atgatttatt atggtcgttt
    22081 aatacaacca ataaaagtat aaatgtttac tctaaatgta ttagtggtaa ggcggtttat
    22141 agttttaatg caggtaaatt catgggcaac tttaatgtta aggaagtaga tgggtgcttc
    22201 atggatgcac aaaagatagc tatagataaa ctattttcta tgctgaaaga cggggttgtt
    22261 ttaaaaggta ataagataaa tgataccatc cttatagaga aggatgggga agttaaatta
    22321 aaattaattc gagggatata attgtattgt gagtaaatat aaaggtctaa atacaagtaa
    22381 tatgttttac atttactcta gtggacatga accagttaac gttgagcttg taaaagataa
    22441 agaacgtaac ataattgagc tggctccagc atggaagggt ttttttttgt ccgaaatcaa
    22501 aatataaaat tcagtgataa tgtgaactat cattatcgct ttaacattaa tagttgtgca
    22561 aaatttcttg ctttttggga ctatttcagt ggtgctctag tagagcatag ccatgctgaa
    22621 aaatgtattc atttctatca tgagaatgat cttcgggata gttgtaatac cgaatcgatg
    22681 cttgataagt taatgttaag attcatcttt tcatctgacc agaatgtaag taatgcactg
    22741 gctatgatac gaatgactga gtcatatcat ttggttcttt atcttctgag aactattgag
    22801 aaagaaaagg aggtaagaat aaagagtctg acagagcatt atggtgtgtc ggaggcttat
    22861 tttagatctt tatgtagaaa agctttaggg gcaaaagtca aagaacaact gaacacttgg
    22921 cggttagtaa atggtttatt agatgtattt ttgcacaatc aaacaatcac ttcagctgcc
    22981 atgaataatg ggtatgcatc aacatcccat ttttcaaatg agataaaaac acgactaggc
    23041 ttttctgcaa gagaattatc gaatattact tttttagtga aaaaaataaa tgaaaaaatt
    23101 taatattaaa tctttgactc tcttgattgt attgttacca ttgatagtta atgctaataa
    23161 tattgactct catttattag aacaaaatga catagcgaaa tatgttgctc aaagtgatac
    23221 ggtaggatct ttttttgaac gtttttctgc gttattaaat tatcctattg ttgttagcaa
    23281 acaagctgca aaaaagagaa tatctgggga gtttgattta tctaatccag aagaaatgct
    23341 tgaaaaactg acattacttg tgggactcat atggtataaa gatggaaatg cattatacat
    23401 atatgactct ggagaattaa taagcaaagt tattttgctg gagaatatat cacttaatta
    23461 tcttattcag tatctgaagg atgctaacct ttatgatcac agatatccta taagaggaaa
    23521 cattagtgat aaaacattct atatatctgg tcctccggca ttggttgaac tggtagctaa
    23581 tacagcaact ttgcttgata agcaggtttc ttctattggg acagataaag ttaattttgg
    23641 ggttataaaa ttaaaaaata catttgttag tgacagaact tataatatga gaggggagga
    23701 tattgtcatc ccaggggtgg ctactgttgt agaacgttta ttaaataatg gtaaggctct
    23761 gagtaacaga caagcccaaa atgatccgat gccacccttt aatattacac agaaagtatc
    23821 tgaggatagt aatgactttt catttagttc agttaccaat agttctatat tagaggatgt
    23881 atcacttatt gcatatccag aaaccaattc tatattagtc aaaggtaatg atcagcaaat
    23941 acagattatt cgcgatatta tcactcagtt agatatagcc aaacgacata tagaattatc
    24001 attatggata atcgatattg ataaatctga attaaataac cttggtgtta attggcaagg
    24061 aacggcttca tttggtgatt cgtttggtgc gtcatttaat atgagctcat cagcatcgat
    24121 aagcactttg gacggcaata agtttattgc ttcagttatg gcattaaatc agaaaaagaa
    24181 agcaaatgta gtttcaaggc cggtgatttt gacacaagag aatattccgg ctatatttga
    24241 taataacagg actttttatg tgtcattagt tggtgagcgc aattcttcgt tggagcatgt
    24301 tacgtatgga acattgataa atgttatacc tcgcttttca tctcggggac aaattgagat
    24361 gtctttaacg atagaggatg gaacaggaaa tagtcagtca aattacaatt ataataatga
    24421 aaatacatct gtccttccag aagttggaag gaccaaaatc agtacaatag cgagagttcc
    24481 acaaggcaaa agtttgttga taggtggtta tacgcatgaa acgaactcta acgaaataat
    24541 atctattcct tttctctcct ctattcctgt aatcggtaat gtgttcaagt ataaaacaag
    24601 taatatcagt aatattgtaa gggtttttct gattcaaccc cgtgagataa aggagtcaag
    24661 ttattataat actgcagaat ataaaagcct catatctgaa agagaaatac aaaagactac
    24721 acaaataatc ccatctgaaa ctactttgct ggaagacgaa aaatcattgg tttcatactt
    24781 aaattactaa ctataaagta ggtgatgtat gcttgatgtt aaaaatacag gagtttttag
    24841 ctctgcattc attgataagt tgaatgcaat gacaaattca gatgatggag atgagactgc
    24901 tgatgcagag cttgattctg gcttggctaa tagcaagtat attgactcat ctgatgagat
    24961 ggcttccgct ctttcgtcat ttataaacag aagagacctt gagaaactga aaggaacaaa
    25021 tagtgatagt caggaacgta ttttagatgg ggaagaagat gaaattaatc acaagatttt
    25081 tgatttaaag agaacgttaa aagataacct tcctctagat cgggatttta tagacagact
    25141 aaagagatat tttaaagatc caagtgatca agtcttagca ttaagggaac ttttgaatga
    25201 aaaagatctt actgctgaac aagtcgaatt attaactaaa attattaatg agataatatc
    25261 aggtagtgaa aaaagtgtta atgctggaat aaattcagct atacaggcta aattatttgg
    25321 caacaaaatg aaacttgaac cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg
    25381 gaacatatca acaacagatc agtatataga atggcttggt aattttggtt ttaatcacag
    25441 acatacaatt gtgaattttg tagagcagtc actgattgta gacatggatt ctgagaaacc
    25501 gagctgtaat gcttatgagt ttggttttgt gttatctaaa ttaattgcaa ttaagatgat
    25561 tagaacttca gacgtaattt ttatgaagaa actggaatcc tcaagcttgc taaaagatgg
    25621 cagtttaagt gcagagcagc tattgctaac tttattatat atttttcaat atccaagtga
    25681 aagtgagcaa attcttactt ctgttataga agtatcacga gccagtcatg aggattctgt
    25741 agtgtatcaa acatatctat cttctgttaa tgaaagtcct catgatatat ttaaaagtga
    25801 aagtgaaaga gaaattgcga tcaatattct acgagagctt gtcacaagtg catacaagaa
    25861 agagctttct agataacagg agataaaagt gatccagtct tttcttaagc aagtaagtac
    25921 taagcctgaa ttaattatat tagtgcttat ggttatgatc attgctatgt tgatcatacc
    25981 attacctaca taccttgttg attttttaat agggcttaat attgtacttg ctattctcgt
    26041 ttttatggga tcattttata ttgaaagaat attgagtttt tctacattcc cttcggtttt
    26101 gttgataacg acattatttc gtctcgcttt atcaataagt actagtcgac taattttagt
    26161 tgatgcggat gcgggtaaaa ttattactac atttggccag tttgtcattg gtgatagtct
    26221 ggctgtaggt tttgttattt tttcaatagt aactgttgtg cagttcattg ttataacaaa
    26281 aggatctgaa agggttgcgg aggtagctgc tcgcttttct cttgacggta tgccggggaa
    26341 acaaatgagc attgatgcgg atttaaaggc tggaattatt gatgccgcag gagctaaaga
    26401 aagacgcagt attctggaac gtgagagtca attgtatggt tcatttgatg gagcaatgaa
    26461 gttcattaaa ggtgatgcaa ttgctggcat cattattatt tttgtcaatt taataggagg
    26521 tatttctgtt ggtatgagtc aacatggaat gtccctatct ggtgcattat ctacttatac
    26581 catactaact attggtgacg gattggtctc tcagattcct gctctgttaa tttctataag
    26641 tgcaggattt atcgttacaa gagttaatgg tgatagcgat aatatgggac gtaacataat
    26701 gtcccagata tttgggaatc cttttgttct cattgttaca tcagctcttg ccttggcgat
    26761 agggatgttg ccaggttttc cgttttttgt ttttttcctg atagcagtta ctttgacggc
    26821 tttattctat tataaaaagg tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag
    26881 tggctatact ggtacatttg atattgataa tagccatgac tcatctttgg caatgataga
    26941 aaatctggat gctattagtt cagaaaccgt tcctttaatt ttattatttg ccgaaaataa
    27001 gataaatgca aatgatatgg aaggtcttat tgaaaggata agaagtcagt tctttattga
    27061 ttatggtgtc aggcttccaa ctattttata tagaacaagc aatgagctta aggtcgatga
    27121 tattgttttg ttaataaatg aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt
    27181 gtgtattaca gatgaaaatg gagatataga tgctctaggt attcctgttg tttcaacttc
    27241 atataatgaa cgtgttattt cttgggtaga cgtttcatat acagaaaatc taactaatat
    27301 tgatgctaaa attaaaagtg ctcaagatga attttatcac cagttgtcac aagctttatt
    27361 aaacaacata aatgagattt ttggtataca agaaacaaaa aatatgttag atcagtttga
    27421 aaatcggtat cctgatctat taaaggaagt cttccgacat gtgactatac agagaatttc
    27481 tgaggtatta caaagattgc ttggagaaaa tatttctgtt cgcaatttaa aacttattat
    27541 ggagtctttg gcgctttggg ctcccagaga aaaagatgtc ataacattag ttgaacatgt
    27601 ccgtgcatca ctttctaggt atatttgtag taaaatagct gtttctggtg agattaaagt
    27661 tgtgatgctt tccggatata ttgaggatgc aataagaaaa gggataaggc aaacctctgg
    27721 tggctctttc ttgaatatgg atatagaggt ttcggatgag gtaatggaaa ctttagcaca
    27781 tgctttgaga gaattgagaa atgcaaaaaa aaatttcgtt cttttggtat cagtagatat
    27841 acgtaggttt gttaaaagac ttatagataa cagatttaag agtatactcg ttatatcgta
    27901 tgctgagatt gatgaagcat ataccattaa tgtattaaag actatttagt gaggtttaaa
    27961 tatgagtaac attaatttag ttcaattagt tagagatagt cttttcacga ttggttgtcc
    28021 tccatcaatt attacagatt tagacagcca ttcagcgatt accatttctt tggatagtat
    28081 gcctgctatt aatatagctc ttgtcaatga gcaggttatg ttatgggcta attttgatgc
    28141 accaagtgat gtaaaacttc agtcatctgc ttataacata ttaaatttaa tgttaatgaa
    28201 ctttagttat agcattaatg agctggtaga gcttcatcgt tctgatgagt acttacagtt
    28261 aagagttgtt attaaagatg actacgttca cgatgggatt gtttttgcag agattctgca
    28321 tgagttttat cagaggatgg aaatcttaaa tggggtctta taatcaatga gctatacaaa
    28381 attgctcact caattatctt ttcctaatag aatctcgggg ccaatcttgg aaacaagtct
    28441 tagcgatgtt tcgattggtg agatttgtaa cattcaggct ggaattgaaa gtaatgaaat
    28501 tgttgcaaga gctcaggttg taggatttca tgatgaaaaa acaatattaa gcttgattgg
    28561 aaattctcgt ggactttcac ggcaaacgtt gattaagccc actgcccagt ttcttcatac
    28621 gcaagtcggc cgtggattat tgggagcagt agtcaatcct ttaggggagg ttactgataa
    28681 gtttgctgtt acagataaca gtgaaattct ttatcgacct gtagataatg ctcctccgct
    28741 atatagtgaa agggctgcaa ttgagaagcc ttttttaaca ggtattaagg ttattgattc
    28801 tttactcacg tgtggtgaag gacagcgaat ggggattttt gcgtcagctg gttgtggcaa
    28861 aacttttctc atgaatatgc tcattgaaca tagtggtgct gatatatatg ttattgggtt
    28921 aattggtgag cgaggtcgag aggttactga aacggttgat tatttgaaaa actctgagaa
    28981 aaaaagcagg tgtgttttag tatatgcaac ttcggattac tcttcggttg atcgttgtaa
    29041 tgctgcatat atagccactg ctatagccga attttttagg actgaaggac ataaagtagc
    29101 gctttttatt gattcattaa caaggtatgc cagagcatta cgtgatgtgg ccttagccgc
    29161 tggagaatca cctgccagaa gaggctatcc ggtttcggtt tttgatagct tacccagact
    29221 tcttgaaagg ccaggaaagt taaaggcagg tggctctatt actgcatttt acactgttct
    29281 tttggaggat gatgattttg ctgatccatt agctgaagag gtaagatcca ttttagatgg
    29341 acatatatat ttgagcagaa atctagccca aaaaggacaa tttcctgcaa ttgattcctt
    29401 aaaaagtata agcagggtat ttacacaggt tgttgatgaa aaacatcgta ttatggccgc
    29461 tgcatttcgg gagctacttt ctgaaataga agagctaagg acaattattg actttggtga
    29521 atacaaaccg ggggagaatg cctctcagga taaaatctac aacaaaatat ctgttgttga
    29581 gagttttctg aagcaagatt atcgactcgg ttttacttat gagcagacaa tggagcttat
    29641 tggtgaaaca attagataag gttttaaaaa tcaaagataa atatcaacgg tctgttaaat
    29701 taattgaagc acacatatta acactaaaaa aaaaactctt tgtatcgaga tgtggaggca
    29761 ttagataaaa ggattatata cttcctgcaa ctggagaatg acttagaacc tgtaggcgct
    29821 cagagtgtta gtcagctttt caatacaaga cgtaagatcg ctatagtaaa aaagcatatt
    29881 atccaatatc agtctgaacg aattcttcta aagggaagaa ttgaagaaat tcaaaaagat
    29941 attgatgagg caaatgcttc taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa
    30001 cggattggct tgattaaaag aaataacttt gccaaacagt tgatacttga tgagttatca
    30061 caagaggata tgaagtatgg cattagacaa tataaaccta aattttagta gtgacaaaca
    30121 gatagaaaaa tgtgagaaac tatcttcaat agataatatt gatagtctcg ttttgaagaa
    30181 gaagaggaag gttgaaattc ctgagtactc tttaattgca tctaactatt ttactattga
    30241 taagcacttt gaacataagc atgataaagg agaaatttat agtggcatta aaaatgcgtt
    30301 cgaacttaga aacgaacgag cgacatattc tgatattcca gaatcaatgg ccattaaaga
    30361 aaatattttg ataccagatc aagatatcaa agcaagggaa aaaataaata tcggcgatat
    30421 gagggggctc ttttcatata ataagagtgg aaatgcagac aagaacttcg aaagaagtca
    30481 tacttcttct gtaaaccctg ataatctgct agaatctgat aatagaaatg gtcaaattgg
    30541 tttaaaaaat catagcttgt ctattgataa gaatattgct gacatcattt ctttactaaa
    30601 tggaagtgtt gctaaatcat ttgagctgcc tgtaatgaat aaaaatactg cagacataac
    30661 cccatccatg tcattgcaag aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa
    30721 aaatagtgaa atgacttacc actttaaaca gtggggggct ggacattctg ttagtatttc
    30781 agtggagtct ggttcttttg ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga
    30841 cttaattttg aaacaagatg ctgagggtaa ttacagattt gatagcagtc aacataataa
    30901 ggggaataaa aataatagta caggatataa tgaacagagt gaagaagaat gctaagaatt
    30961 aaacattttg acgctaacga aaaactacag attttatatg caaagcaact ctgcgagcgt
    31021 ttttcgattc agacattcaa aaataaattt acaggcagtg aaagtttagt cactcttact
    31081 tccgtgtgtg gggattgggt aattcgtatt gatacattat cttttttgaa aaaaaaatac
    31141 gaggtatttt caggattttc tacacaagaa tctttactgc atttatcaaa atgtgtcttt
    31201 atagagtcgt catctgtatt ttcgattcca gaactgtctg ataagattac tttccggatc
    31261 acgaatgaaa tccagtatgc aactactggg agtcatttat gctgtttttc ctcttcttta
    31321 ggtattattt attttgacaa gatgccggta ttacgtaatc aagtttctct tgactcattg
    31381 catcatcttt tagagttttg cttaggttca tctaatgtaa ggctggctac tttaaaaaga
    31441 attcgcactg gtgatataat catagttcag aaactttata atttattatt gtgtaatcaa
    31501 gttattattg gggattatat tgtgaatgat aataatgagg caaaaattaa tctgtcagaa
    31561 agtaatggtg agtcagaaca cacagaagtt tctttggcat tattcaatta tgatgatatc
    31621 aatgtaaaag tggactttat tcttttagaa aaaaatatga caatcaatga actaaaaatg
    31681 tatgtagaaa acgaattatt caagtttccc gatgacatag ttaaacatgt aaatattaaa
    31741 gtaaatggtt ctttggttgg gcatggggaa cttgtttcta ttgaggatgg ttatggtatc
    31801 gagattagtt cttggatggt aaaggagtaa tctcatgctg agtgacatgt ccctcatcgc
    31861 tacactttct ttttttacgc tattaccatt tttagttgct gctggtacct gttatataaa
    31921 gttttctatt gtttttgtaa tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa
    31981 tatgactctc aatggtattg cattgattat ggctttgttc gtgatgaaac ccattattga
    32041 agctggttat gaaaattatc taaatggccc tcaaaaattt gatacaatat ctgatattgt
    32101 ccgtttttct gattctggct tgatggaata taagcaatac ttgaaaaagc atacagatct
    32161 tgagttagca cgttttttcc aaaggtctga agaagaaaat gctgatttaa agagtgcaga
    32221 gaataatgat tattcattgt tttccctctt gcctgcatat gcgctaagtg aaatcaaaga
    32281 tgcgttcaaa ataggttttt atttatattt gccatttgtt gttgttgatc ttgttatttc
    32341 aagcatttta cttgcacttg gtatgatgat gatgagtcca atcacaatat cagtaccaat
    32401 aaaacttgta ttatttgttg cgcttgatgg gtggggtatc ctgtctaagg ccttgattga
    32461 acaatatatc aatattcctg cttagatgat ggaggtaatg tgtctgatat agtttatatg
    32521 ggtaataagg ctctttattt aatccttatc ttttccttat ggccagtagg tatagctacg
    32581 gttattggat tatctattgg tttgttacag acagtgactc aactccaaga gcagacactt
    32641 ccttttggta taaagcttat aggtgtctca atatctttgc tacttctttc tggatggtat
    32701 ggtgaggttt tattgtcttt ttgtcatgaa ataatgtttt taattaagag tggggtttga
    32761 tggacatttc aagctggttc gaaagtattc atgtgttttt aatactcctg aacggcgttt
    32821 tttttagatt agctccatta tttttctttc ttccattttt aaataacggt ataatttctc
    32881 catctattag aatacctgtg atttttcttg ttgcatcagg attaattact tctggtaagg
    32941 tagacatagg ttcttctgtt tttgaacatg tttatttcct tatgttcaag gaaataattg
    33001 ttggccttct tctctctttt tgcttgtctc ttcccttttg gatatttcat gctgttggta
    33061 gcattattga caaccagcgt ggggcaacgc ttagtagttc aattgatcct gccaatggtg
    33121 ttgatacgtc tgagttggca aaatttttca atcttttttc tgcagttgta tttctataca
    33181 gtggtggtat ggtctttatt ttagaatcca tacaattgtc ttataatata tgcccgttat
    33241 tttctcaatg ttctttccgt gtctcaaata tcttaacatt tctgacttta ttggcaagtc
    33301 aggctgttat tttagccagt cctgttatga tagtattgtt actatcagaa gtattacttg
    33361 gtgtattatc gagatttgct ccgcagatga atgctttttc cgtatcatta actattaaaa
    33421 gtttacttgc aatatttatt attttcatct gttcttctac tatttacttt tctaaagttc
    33481 aatttttcct cggcgaacat aaatttttca caaatctatt tgttagataa aatattatgg
    33541 caaataaaac agaaaagccg acacctaaaa aactaaagga tgccgcaaaa aaaggacagt
    33601 catttaaatt taaggattta acgactgttg ttattattct ggtagggaca tttactataa
    33661 tatcattctt ttccttaagt gatgtaatgc tcttatacag atatgtaata attaatgact
    33721 tcgaaattaa tgagggtaaa tacttttttg cagtggttat tgtctttttt aagataattg
    33781 gcttcccact ttttttctgt gttctttcgg ctgtgttgcc aacattggtt caaacaaagt
    33841 ttgttcttgc gactaaagct atcaagattg atttttcagt attaaaccct gttaaagggt
    33901 taaaaaaaat atttagtata aagacaataa aagaattttt caaaagcatt ctgcttctta
    33961 ttattctagc attaacaacc tatttctttt ggattaatga ccgaaaaata attttttctc
    34021 aggtgttttc tagtgttgat ggcttatatc ttatttgggg gaggctgttt aaggatataa
    34081 tattattttt cttggcattt tctattcttg ttattattct tgactttgtg attgagttca
    34141 ttttatacat gaaagatatg atgatggata aacaggagat aaaaagagaa tatatagagc
    34201 aagagggaca ctttgagaca aagtcgagaa ggcgtgagtt gcatatcgag attctttcag
    34261 agcagactaa atctgatata cgtaattcaa aattagtggt aatgaacccg actcatattg
    34321 caattggtat ttattttaat ccagaaatag cgcctgcacc ttttatttct ctcattgaaa
    34381 ctaaccagtg tgccttggct gtcagaaaat atgcaaatga agttggtata ccgactgtgc
    34441 gtgatgtgaa attagctaga aaactatata aaacacatac aaaatatagt tttgttgatt
    34501 ttgaacactt ggatgaagtc ctacgtctta ttgtttggct tgagcaggtt gaaaacactc
    34561 attaataagt aaaggagatg tgttatatgg gagttaattt ctgtaataaa ataggtattg
    34621 atcagtctga atttgaaata gaatcttcca tcattaactc cattgctaat gaggtattga
    34681 acccaatatc tttccttagc aataaggata taataaatgt tttactcaga aagatttcat
    34741 ctgaatgtga cctggttaga aaagacattt atcgttgtgc tctggagttg gtcgttgaaa
    34801 aaacacctga tgatttataa tcattcataa taactctttt gctgatataa atatttttgt
    34861 tgaagatatt tatgtagtca caggatcaat tgtagaaaat tgttatgttt ttgttaggaa
    34921 ctgcactaaa taatatatga taagacagca aaaacgactg accataatcc ttcttttatt
    34981 aggagttgat aagagagact attcatcctg taatgttaaa actctgttat atagtattcg
    35041 cgattatgca aagtctgtaa atgatcatga gatactaacc gagagtaatc gtttattatc
    35101 tcattgcatt tcagatagta acggagcttt ctttaaaagt agtaaatacg ttccccttaa
    35161 gtacttacgt aaaaggagaa tagcacga
//
LOCUS       DAJTNW010000033        34996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNW010000033 DAJTNW010000000
VERSION     DAJTNW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..815)
                     /locus_tag="OO179_001737"
     CDS             complement(<1..815)
                     /locus_tag="OO179_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5531836.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO179_001738"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO179_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OO179_001739"
     CDS             complement(1398..1874)
                     /locus_tag="OO179_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5531837.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO179_001740"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO179_001740"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5531838.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OO179_001741"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OO179_001741"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5531839.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OO179_001742"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OO179_001742"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5531840.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OO179_001743"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OO179_001743"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5531841.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OO179_001744"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OO179_001744"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5531842.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OO179_001745"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OO179_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5531843.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO179_001746"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO179_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5531844.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OO179_001747"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OO179_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5531845.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OO179_001748"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OO179_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5531846.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OO179_001749"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OO179_001749"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5531847.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OO179_001750"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OO179_001750"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5531848.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OO179_001751"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OO179_001751"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5531849.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OO179_001752"
     CDS             13849..14553
                     /locus_tag="OO179_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5531850.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OO179_001753"
     CDS             14758..15471
                     /locus_tag="OO179_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5531851.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OO179_001754"
     CDS             complement(15507..16463)
                     /locus_tag="OO179_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5531852.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO179_001755"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO179_001755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5531853.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OO179_001756"
     CDS             complement(17701..18462)
                     /locus_tag="OO179_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5531854.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OO179_001757"
     CDS             18595..19005
                     /locus_tag="OO179_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5531855.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OO179_001758"
     CDS             complement(18967..20073)
                     /locus_tag="OO179_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5531856.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OO179_001759"
     CDS             complement(20084..21217)
                     /locus_tag="OO179_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5531857.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OO179_001760"
     CDS             complement(21210..22946)
                     /locus_tag="OO179_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5531858.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYHGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO179_001761"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO179_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5531859.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO179_001762"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO179_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5531860.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OO179_001763"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OO179_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO179_001764"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO179_001764"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5531861.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OO179_001765"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OO179_001765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5531862.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OO179_001766"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OO179_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5531863.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OO179_001767"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OO179_001767"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5531864.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OO179_001768"
     CDS             complement(31428..31688)
                     /locus_tag="OO179_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5531865.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO179_001769"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO179_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5531866.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO179_001770"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO179_001770"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5531867.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="OO179_001771"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="OO179_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8296 a         8973 c         9455 g         8272 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctagcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttgccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aacaggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgaccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaat gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccatggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 attatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aaccatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DAJTNW010000034        34952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNW010000034 DAJTNW010000000
VERSION     DAJTNW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..2263
                     /locus_tag="OO179_001772"
     CDS             <1..2263
                     /locus_tag="OO179_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5531868.1"
                     /translation="DAKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGI
                     TENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGG
                     NFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFY
                     LNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKT
                     TSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAIS
                     ASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISA
                     TVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     IVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
     gene            2260..3348
                     /locus_tag="OO179_001773"
     CDS             2260..3348
                     /locus_tag="OO179_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5531869.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3436..4734)
                     /gene="exuT"
                     /locus_tag="OO179_001774"
     CDS             complement(3436..4734)
                     /gene="exuT"
                     /locus_tag="OO179_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5531870.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5218..6630
                     /gene="uxaC"
                     /locus_tag="OO179_001775"
     CDS             5218..6630
                     /gene="uxaC"
                     /locus_tag="OO179_001775"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5531871.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6645..8132
                     /gene="uxaA"
                     /locus_tag="OO179_001776"
     CDS             6645..8132
                     /gene="uxaA"
                     /locus_tag="OO179_001776"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5531872.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8215..8766
                     /locus_tag="OO179_001777"
     CDS             8215..8766
                     /locus_tag="OO179_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5531873.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8771..10015)
                     /gene="sstT"
                     /locus_tag="OO179_001778"
     CDS             complement(8771..10015)
                     /gene="sstT"
                     /locus_tag="OO179_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5531874.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10611..11576)
                     /gene="alx"
                     /locus_tag="OO179_001779"
     CDS             complement(10611..11576)
                     /gene="alx"
                     /locus_tag="OO179_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5531875.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11847..12845)
                     /locus_tag="OO179_001780"
     CDS             complement(11847..12845)
                     /locus_tag="OO179_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5531876.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12924..13616)
                     /locus_tag="OO179_001781"
     CDS             complement(12924..13616)
                     /locus_tag="OO179_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5531877.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13689..14192)
                     /locus_tag="OO179_001782"
     CDS             complement(13689..14192)
                     /locus_tag="OO179_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5531878.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14277..15413
                     /gene="rlmG"
                     /locus_tag="OO179_001783"
     CDS             14277..15413
                     /gene="rlmG"
                     /locus_tag="OO179_001783"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5531879.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15698..16012
                     /gene="higB"
                     /locus_tag="OO179_001784"
     CDS             15698..16012
                     /gene="higB"
                     /locus_tag="OO179_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5531880.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16009..16425
                     /gene="higA"
                     /locus_tag="OO179_001785"
     CDS             16009..16425
                     /gene="higA"
                     /locus_tag="OO179_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5531881.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16470..18488)
                     /gene="fadH"
                     /locus_tag="OO179_001786"
     CDS             complement(16470..18488)
                     /gene="fadH"
                     /locus_tag="OO179_001786"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5531882.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18714..21065)
                     /gene="ygjK"
                     /locus_tag="OO179_001787"
     CDS             complement(18714..21065)
                     /gene="ygjK"
                     /locus_tag="OO179_001787"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5531883.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21082..21501)
                     /locus_tag="OO179_001788"
     CDS             complement(21082..21501)
                     /locus_tag="OO179_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531884.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21479..21832)
                     /locus_tag="OO179_001789"
     CDS             complement(21479..21832)
                     /locus_tag="OO179_001789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531885.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21833..22153)
                     /locus_tag="OO179_001790"
     CDS             complement(21833..22153)
                     /locus_tag="OO179_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531886.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22287..23720)
                     /locus_tag="OO179_001791"
     CDS             complement(22287..23720)
                     /locus_tag="OO179_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5531887.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23783..24232)
                     /locus_tag="OO179_001792"
     CDS             complement(23783..24232)
                     /locus_tag="OO179_001792"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5531888.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24229..27306)
                     /gene="ebgA"
                     /locus_tag="OO179_001793"
     CDS             complement(24229..27306)
                     /gene="ebgA"
                     /locus_tag="OO179_001793"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5531889.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27489..28472)
                     /gene="ebgR"
                     /locus_tag="OO179_001794"
     CDS             complement(27489..28472)
                     /gene="ebgR"
                     /locus_tag="OO179_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5531890.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28690..29021
                     /locus_tag="OO179_001795"
                     /pseudo
     CDS             28690..29021
                     /locus_tag="OO179_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29063..30442)
                     /gene="ygjG"
                     /locus_tag="OO179_001796"
     CDS             complement(29063..30442)
                     /gene="ygjG"
                     /locus_tag="OO179_001796"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5531891.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30860..32380
                     /gene="aer"
                     /locus_tag="OO179_001797"
                     /pseudo
     CDS             30860..32380
                     /gene="aer"
                     /locus_tag="OO179_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32534..33157)
                     /gene="nfeR"
                     /locus_tag="OO179_001798"
                     /gene_synonym="yqjI"
     CDS             complement(32534..33157)
                     /gene="nfeR"
                     /locus_tag="OO179_001798"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5531892.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33434..34179
                     /gene="nfeF"
                     /locus_tag="OO179_001799"
                     /pseudo
     CDS             33434..34179
                     /gene="nfeF"
                     /locus_tag="OO179_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34233..34308)
                     /locus_tag="OO179_001800"
     tRNA            complement(34233..34308)
                     /locus_tag="OO179_001800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34273..34275),aa:Ile,seq:cat)
     gene            34433..34939
                     /gene="mug"
                     /locus_tag="OO179_001801"
                     /pseudo
     CDS             34433..34939
                     /gene="mug"
                     /locus_tag="OO179_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8542 a         8979 c         9180 g         8251 t
ORIGIN      
        1 cgatgcgaag tcattatatt taaatccaga caaaaatact gttaacgctt ttatacatca
       61 gcaagacatc aatgttctgg cacaggatga ttaccaatcg ttgtctattc agggaaacgg
      121 tgcgctggga ataacagaaa atagctatat tggtgcacac tggaatttca acggttatga
      181 tgcagatgat gtcagtgaca gtaatactga cgtcagcgat ctctattatc gttatgattt
      241 tttacgtcgt tattatgtgc aggcgggtcg catggacaac cgcacactat ttaatgcaca
      301 aggcgggaac tttaccttta actttctgcc actcggtgca atcgatggga tgcgtatcgg
      361 gtcgacactg agctatttaa accaggcgca aagccagcaa ggaaccccag taatggttct
      421 gctttcgcgc aattctcgtg ttgacgctta tcgtaatgag caacttttgg gatcgtttta
      481 tctcaatagt ggttcgcaat ttattgatac cagttccttt ccgccaggca gctacagcgt
      541 ggcattaaaa gtctacgaaa ataaccaact cacccgcacc gagcttgtgc cgtttaccaa
      601 aaccggcggt ctgactgacg gaaatgcgca atggttctta caggcaggta aaactacatc
      661 acaggtttct gatgatgaaa gttcagctta tcagctagga gtacgcctgc cattacatcc
      721 gcaatatgag ctctacgcag ggctggcgaa tgccgatgat gtgagtgctt tcgagttagg
      781 taataactgg acggcagatt taggcagggt ggggaatctt gcaatcagcg ccagcgtgtt
      841 ccgtaacgat gacggcggca aaggtgatat gcaacaggcc aactggagta atccgggatg
      901 gccgacgttg ggcttttatc ggaccaactc tgacggcgat gcttgtacaa ccgacagcag
      961 agagagctat aacgccttaa gctgttatga aagtatttcc gcgacagttt cactgaattt
     1021 tgtcggctgg aatatgatgc tgggttatac ctgcacacaa aataacactg atgatagttt
     1081 gcgttgggat aaacagcaga gctttgaaaa taactatctt cgccagacaa cagcgcaaag
     1141 tatctctgaa actgtacaac ttagcgcttc ccgcgctatt gtgatgcgtg actggatttt
     1201 gagtacgtca gtcggtgttt tccatcgtaa tgacaacggt ggcgataacg acgacaacgg
     1261 cttgtactta tcgttttcgt tatctgacac gccaacgatg gatagcaata acaacagcca
     1321 ttcaaccaat gtgcctacgg attatcgtta tagcgaacag gatggcgatc aaacgtcatg
     1381 gcagttatcg catacctttt ataacgattc attcagccat aaagaacttg gcgtaaccgt
     1441 cggaggcctg aacaccgata ccataaacag cgcggttaac gggcgttggg atgggcaata
     1501 cggaaatgtc tacgctaccg tatctgatag ttatgaccgt aagaatcatg atcatctttc
     1561 ggcctttacg ggcacttata gctctacgct ggctgtcagt cgctatggcg ttaatttggg
     1621 agccagtggt acagacgatt tgctgggtgc ggtactggtg gatgtgaaag gcttctctga
     1681 acaggatgaa gagagtcagg atctgcaact cgaagcgcgg gtggcaggca gccgaacttt
     1741 gcagcttggt caaagtgaca gtgtgttgtt cccttatcct ggatttcagt ctggttttgt
     1801 tgaggttaac gacagtagcc agggcaatca gcaggggaca acaaacatca ttaacggtgc
     1861 agggaatcgt gaattaatgt tgttgcctgg caagctgcgc tatcgcgaag tgtctgccag
     1921 ctttaactac aactatatcg gtcgtttatt actgccggca gcggtgaaaa aattcccgat
     1981 tgtggggctg aacagcgcca tgttactggt agctgaagat ggcggattta cacttgaaat
     2041 taacggcagc gaaaaagagc tctatctgct ttccgggcag caattcctta agtgtccact
     2101 gagtgttgta aagaaacgcg ccagcattcg ttacagcgga gatgttactt gtagtgtggt
     2161 gacttattca caattaccgg agtcgattca ggttcaggca cagttgaaac agcctaaatt
     2221 acgtggaaac gttcagacgg cgcaaaggga ggttgcacca tgagaaatcg attgattgcg
     2281 gcgatattgg gcttgtttgg cacgctcact ggcgttcagg cagctcctga cgtgaccagt
     2341 gaaattacgt atgatttggc atctggtaga gcggattatt acttctggaa ggatgaagcg
     2401 tcggcaggaa ataatggata tatgtggtat gaatgttcgt atcctgacct ccaacaaacc
     2461 tgtacagcta atggaaatat atcgacagta caaatctatt taactgaaca acgcagtggg
     2521 atgcgttggc cggtaaaact caaaggattt aaaacagcca ttgtaagtag cgatgaagcg
     2581 ccgctaggat gcaagggggg caaagggctt cagacgaatc ttaaggattc taatagatct
     2641 tcatgtacag aagatggtca acattattat atatacgata caaagtttct tacgctctac
     2701 cttgagcaga cagagatgaa gaatttgccg attggtggcg tctggaaggg gaaagttaaa
     2761 ttacattcga acagcccggc ccaggactat ttcgcaaata ttaccctgaa tacgctcgac
     2821 cccaaccata ttgacgtgtt cttcccggag ttcgcccacg ccacgccaag ggtgcagtta
     2881 gacttgcatc caacaggaag cgttaacggc agcaactacg cgcaagatct gaccatgttg
     2941 gacatgtgcc tgtacgatgg ttttaacggt aatgccatca gttatgaaat catgctcaaa
     3001 gatgaagggc gaccagctgc agggcgcaga gacggttact tctctatcta tcgtcaggga
     3061 gggaccacca ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa
     3121 accggtgggc aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg
     3181 aaacgtgtgc gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca
     3241 cccttgactc tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg
     3301 ggcaaattgt cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg
     3361 gacaaacgct tttttctata taaacgacga agccgcctga taaggcggct tcgtcgttat
     3421 aacttacgtt aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gcgccacctc
     3481 aatcgccggt ttgttctgca atacggtcca gataaccagc gcacccagca ggtcaaatac
     3541 tgccagaact gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag
     3601 cgcaaacagc gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac
     3661 ttcgttacga ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg
     3721 ggcaaaacca ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat
     3781 catacccggg ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat
     3841 caggttcaca ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc
     3901 gaggtcagca aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc
     3961 gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc
     4021 aaggaaacgc ggcagtgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga
     4081 cattttcttc gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg
     4141 ttcttcatcg gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat
     4201 cgcccagata aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcatcac
     4261 gattgcccat accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa
     4321 gtagcctact gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc
     4381 cgccgggatc atcgctgctt ccgcggcacc gaccgcacca cgagcaacag ctaggccacc
     4441 ccagctacct gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc
     4501 ataaccgatt ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt
     4561 ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc
     4621 ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt
     4681 gccgagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa
     4741 cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat
     4801 gttagttacg accgggtaac cgaaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     4861 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     4921 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     4981 ccttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     5041 agcgaggcca gtactggcgc ggcctgcagc gagatttacc actttgagag taattttttt
     5101 aactacgttt attgatctaa ctcaagaaaa tatcttcgga ctctggaaat tggtgtgata
     5161 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     5221 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     5281 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     5341 gcggaagact atcgttttaa aaacctgtat gacatctggt tgaagggcga tcactacaaa
     5401 tggcgcgcta tgcgtaccaa cggtgtggct gagcgtctgt gtactggtga tgcgtctgac
     5461 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     5521 cactggacgc acctcgaact gcgtcgtccg ttcggtatca ctggcaaatt gctttctccg
     5581 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     5641 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     5701 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     5761 ccgagctggc gtccggacaa agccttcaat atcgaacagg cgacctttaa cgactacatg
     5821 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     5881 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     5941 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     6001 ctggctggcg aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     6061 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     6121 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     6181 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     6241 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     6301 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     6361 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     6421 ctgctgagcc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     6481 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     6541 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     6601 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     6661 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     6721 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     6781 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     6841 tggcggatat tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg
     6901 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     6961 ctgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga
     7021 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     7081 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctatggct
     7141 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     7201 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     7261 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     7321 agcaggatga tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc
     7381 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     7441 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     7501 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     7561 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     7621 acgacttcaa acagtacttt attgcccatg atcagccgat ctacgaaaac ccatcaccgg
     7681 ggaacaaagc gggcggtatc accacgctgg aagacaaatc acttggctgt acccagaaag
     7741 cgggttccag tgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccagggc
     7801 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     7861 gctgccatat ggtgctgttc agtactggtc gcggcacgcc atacggtgga tttgtgccga
     7921 cggtgaaaat cgccaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     7981 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     8041 ccatcgttga gtttgccaac ggtaagcaaa cctgcaacga gcgtaacgac ttccgcgaac
     8101 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     8161 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gctaaggatt tatcatgacc
     8221 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
     8281 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
     8341 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
     8401 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
     8461 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
     8521 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
     8581 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcacg
     8641 atgattgagg gtagttttct gcttatgaat ggcctgaata tcattcgttt ctggcggatg
     8701 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccat agacgaaagg
     8761 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
     8821 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
     8881 atacgccgat aataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
     8941 acatattaca ggccagtggg atcagcagca aagaaccccc cgccacgccg gatgcgccac
     9001 aggcacacag agaagccact acgctcaaca gcagcgctgt gggcagatcg accggaatac
     9061 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
     9121 tgatggtggc tcccagcgga atagaaactg aataggtatc gcgatccaga ttcagctttt
     9181 cacacagcgc catattcacc ggaatgttcg ctgcagagct gcgggtgaag aaggcataca
     9241 caccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
     9301 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
     9361 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
     9421 gcccaaaaat accgaacggt gcgaagcgaa tgaccagttt caccataaag gtaacggcat
     9481 tcgacatatc attgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaagccga
     9541 ggccgatcgc ccacaccaga atcccgatgt agttaccttt cagcagggcg tcgatggggt
     9601 tggaaaccat gctcattacc agtccgcgca tcacttcgac aatgcctgac ggcggcgaaa
     9661 tatcacccgc gctactggac aagtgcaggg tagaagggaa ggcaaagctg aagactactg
     9721 cggccagagc tgcagagaag gtgcccagca gatagaggaa caaaatagga cggatattgg
     9781 ttttctgccc gtgctggtgg ttagcaatag atgccattac cagcatcaac accaggatgg
     9841 gggcaacagc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
     9901 cgggttttga gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
     9961 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
    10021 gatcctttca ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
    10081 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
    10141 gctggatttt aaagagaggt catgttttgc aacttaatgg ttgcatttat ttaacataaa
    10201 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
    10261 tatgagaacg gttgcgctcc tttaacgtgt tatgccatat aaagtcactg gcgcagggag
    10321 cgcgcagggg gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat
    10381 cgccgctgcg cggttccctc agcctatccc ttgcggctac cgggtcgggc gcaaggtaac
    10441 gtccctgtaa gatgcgccct cgccccggcc tccagggaac gcttcggcga ttttgacgcc
    10501 accaacaccc gtgcggtctt tttgtaaaga gctataagcc gattacataa ttaatagctc
    10561 acctttgtct ggaatgagtt atctctagga atggcttaat atacagcgaa tcattctccc
    10621 cgctgcttat catgccgata attcacccag gcgttgataa taaacgtcat caccagaatg
    10681 ccaaacacca cgcccagcga gacggcgatt ggaatatggt agaagtcgac aatcagcatc
    10741 ttgataccga taaacaccag aatcaccgcc aggccatatt tgagcatcga gaaacgctct
    10801 gctacgcccg ccagcaggaa atacatcgca cgcaggccga ggattgcaaa caggtttgag
    10861 gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag ccggaatgct atccacggca
    10921 aaaatcacgt cgctcaattc caccagaatc agtaccagca tcagcggtgt ggcgtacagc
    10981 aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga
    11041 ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg attcatcttc atgggcgagc
    11101 gccatcttca cgccggtaaa cagcaggaag gcaccgaaga tatacaggat ccagtcgaac
    11161 tgcgaaatca gccagctgcc tgtgaagatc atgatggtac gcagaacaat cgccccgagc
    11221 acgccataca ccagcacgcg acgttgtaat gccgccggaa cggagaaata gctgaacaac
    11281 atcagccaga caaagacgtt atcgaccgcc agcgatttct caatcagata acccgtgaga
    11341 aaggccaggg cctgtggatc ggcaacggcg cgaccttcgg tttgcaccag ataccaccag
    11401 aaagcggcgt taaacagtaa cgacagcgtc acccagacca gcgaccaggc cgcagcctgt
    11461 ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca acaggtcgat agccagcata
    11521 atggcgacaa caacagcgaa tccgccccat agcaacggcg tgccgacagt attcatagaa
    11581 gttccttaca cataaaaaaa cggccaacgt cggaagacgt cagccgctgc tttttatgca
    11641 tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc
    11701 ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg
    11761 caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag tcagtcaatg
    11821 gtctgttaca taccttttac actgttttac tttggcaagt atagttttac gctatctgcc
    11881 ggaaaaatca ccccagtttg gcggcggatc tcggtcagca gtttggctgt gatgcgactg
    11941 accgccagcc ctggatgatc caccagatgc tcatccacca gggtagcgaa cagctctgct
    12001 tcgtagagca tggtattaat atgctgcggc tgggtgagat cctgcatctg gctgccacgc
    12061 ggcacaaagc agactttctg gcattcagac agtttttcaa tcaccagcga tcccgcttcg
    12121 ccctgaatct cgcttcccag gacagaatca ctgacctttg agtgctgcaa ggtgacgctg
    12181 aaatcaccgt aatccatcac caccacgccg tgggcgtcaa cgccgcttgc cagcaaactc
    12241 gccgttgcct gcacgctttt cggctcacca aataacgcca ccgccgacgc caggcagtaa
    12301 aagccgatat ccataattga accgttagag aatgccggat taaaggtgtt gggattctca
    12361 ccgtccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa gactttacgc
    12421 agtttgcctg ctttcggcag cgcctggcgc aacaaatgaa agttcggcag gcaggcggtt
    12481 ttaaatgcct caaacagcac cacctgattt tcgcgcgcac aggcaatggc ggcatccact
    12541 tccgccagat tcgacgccag cggtttttcg caaatcacat gaattttatg gctaaggaaa
    12601 agttgtgtct gggaaaagtg cagggaattc gggctggcaa tatacaccgc gtcaatggca
    12661 tcgctttccg ccatcgcttc cagcgacgta aacagatgct cgacagaaaa atcattggcg
    12721 aagtgctgag cctgctcaag gctgcgggaa tatacggcgg ttaacttgta tttaccgctc
    12781 tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg taccaatcac agcgaaacgt
    12841 atcataacgc gtggatactc cataaaggga ttctgtcgtc aatgtagcac gcctttttca
    12901 tcttcgacgt gcgcagatac gcattattct ttcgccggac agccatgctc actaaacggc
    12961 ccaatcatga ccgatggccc gagatatttc ggctggcgat ggaggatttt tgcgtcaatc
    13021 accgcgctga cacgagcgag tttctcccgc agttgagtat aaaaaccgtg cttcatattg
    13081 aggtctggcg cgttaaaccc gatagcgtcg atgccgtact gttttgccag ccagatggcg
    13141 cgttggttat gaaattcttg cgagataatg gtgatatggt tttccccaaa cacctttttg
    13201 gcacgcacta ccgaatccag cgttgagaat ccggcatagt cgcaaaagat cactttcgcc
    13261 ggtacgcctt tagcgatcaa agcctgctgc atacctgatg cttcatcgta atttttacgt
    13321 ccgttatcgc cactcaccag tagccatttc acttttccgg cgtggtacaa cgctgctgcg
    13381 gtatcaatac gtcgggtaaa gtagcgatta cccggcctcg cccccagcaa taaacccacg
    13441 ttacgcgccg ggacggcgtt gacatcgccc caggtcagct gcttgctggc gttgaccata
    13501 acgcgatcgg cgataaaaat ggtcgctcct gccacaagta acagtaagca ggcaatcttt
    13561 agagtccggc gagaaaaaca aatacggaga agaaggcggg caaatgcgcg cagcatggtc
    13621 gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaaaga taacgcctga
    13681 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    13741 aactcaagct ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta
    13801 tgctctttct ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc
    13861 ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc
    13921 tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg
    13981 tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga
    14041 gtatattgcc agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    14101 agcacatcgc ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg
    14161 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    14221 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    14281 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    14341 ttaacccgct acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca
    14401 cagaaatccc cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc
    14461 tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg
    14521 agaatttacg cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    14581 actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
    14641 aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
    14701 ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga
    14761 ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc
    14821 cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
    14881 ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc
    14941 aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta
    15001 ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
    15061 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc
    15121 gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg
    15181 tgctctgcaa ccctccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga
    15241 tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    15301 gccatctgga ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga
    15361 cgaataataa atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg
    15421 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttacccct
    15481 aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc
    15541 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    15601 ctacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt
    15661 tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc
    15721 attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa
    15781 cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct
    15841 ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg
    15901 tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca
    15961 taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt
    16021 gccgacatct tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt
    16081 cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat
    16141 cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg
    16201 cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt
    16261 cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa
    16321 tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa
    16381 ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat
    16441 gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg
    16501 gcaattgccc gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca
    16561 gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag
    16621 atcaccacat tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg
    16681 tcatcgtcaa tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg
    16741 atggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg
    16801 cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag
    16861 ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc
    16921 tggctggtgg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca
    16981 caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa
    17041 ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg
    17101 gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc
    17161 agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc
    17221 tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca
    17281 aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt
    17341 ccagcgccga ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt
    17401 tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg
    17461 aggcagccct ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct
    17521 tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg
    17581 ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc
    17641 ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg
    17701 ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc
    17761 gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct
    17821 tcgaccaggt cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg
    17881 cgcaccgtac gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac
    17941 tggtcactac gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag
    18001 cccatcactt cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa
    18061 tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg
    18121 ggggcctgca atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc
    18181 aaaatttgca gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg
    18241 tgtgggatct ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctgttaaa
    18301 tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa
    18361 aacgcagcca gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc
    18421 atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg
    18481 tagctcataa aattgtccag tatgtaaaat aattgttatg tagtcggatg agttctaatt
    18541 tagccgtcga tggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag
    18601 aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg ttttatccgg cttgggggat
    18661 tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagccgttgc aaattactgc
    18721 ttacggaaaa aatcgttata caacatatac aaatgagcgg cactccagga gaaatttggt
    18781 gcgccttgct gcgcgcctgt cagcgggttg taattctcct gaattgggcc atcggcggtt
    18841 aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca gggcatcatc acgataaccg
    18901 taacgctcca tccctttcag accaaaccag aactgatcca cccatacgcg cccgcgccag
    18961 tagatatcag cgccaaaagc cggattggtt aacgccgccg ttcccagcgg aacaaaggtg
    19021 ttgaactctt taggatcgag catcaccttc accaccgcgt cggcattggc ctgcgttgcc
    19081 gcaccgttaa acagcggcga ccagccttcc ggccctttac cgcgctcaac aatcggtttg
    19141 cccgcgcagc cgttcgccag tggtttatct tcaatacgta cgtcatagta gaactgggta
    19201 gtcgggtcga acatacaggt gttgatgtag tccgcgagct gctgtgccaa ctggcgatag
    19261 cgtttggcct cttctggctt accgaggatg gttgccatct ccgccagata atggttatcg
    19321 ctgtacatat agctggcctg atccaccgac tcctgcaata gcgagtagcc cagcaacgtt
    19381 ccgtcctgac tgcggttttc ggcgaatttc accgtccagt cgctacgttt gccgccatta
    19441 gcgacatatt tatccaactg ttctttgtcg ataaaaccaa agacggcggc atcatcacgg
    19501 cccgattccc acgacgcagc gacctgtgcc ggaatttcca gactgtcgta ctggcctttc
    19561 tccaccacgc gggcgtagtt gttcagccca gactgcgtct cttctttatc gccttttttc
    19621 accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt tgtcgcgggt cgcgccatat
    19681 tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc accagtcgtg ataggccacc
    19741 agtttcgggt acatctctgc caaccaggct ttatcctggg tgacgttgta tacttccatc
    19801 accgaccagg cggcaaggct gggtttggta ttacgttcgt tccagttgcc gccatcgcca
    19861 ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga caaagcccac atcctgcgga
    19921 cgcacgctgt cgccaggctg gatctgccag gagaagaccg cgcggatatt ctctttggcg
    19981 atatccggat tgaaatgcgc catcgcaaac gcctgcttcc aggtatccca cggccaggtc
    20041 tgattgccgg agaaccagcg tccggtcacc gacggcgtga cggtgttata attcaccgca
    20101 ccgcccggtg agcgccagtt accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc
    20161 tgttccggcg tcgcatccgg attggttaac cctttcttca ggtattcttc ccagcgttgc
    20221 tgcgaggcgg tgagataaaa cgccggacgc gccagaatat cgcggatctg catttgctct
    20281 ttgctaactt cctgagcggt cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg
    20341 ttgatatgcg ccttactggt aaagcgatta ccgttgattt cagtctgcac cggcagggat
    20401 ttatgcacct gatattccga ttcgccggag gtcagcagat cccaggtggc gcgcactttg
    20461 ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt tgcgctgata gtcagggtat
    20521 tcgccagcaa tggttttatc ggaaagcggt ttcccttctt tcgcttccag tttttccagc
    20581 agttcgccat cccacaccag atccagcggt ttattgctga tgattttagt ttccagtagt
    20641 gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct gcacatcttt tgctgtcagt
    20701 ttttgcacca gcgcgccggg aatactgtat gcctccagcg tgaagttgac tttcttgccg
    20761 tcttgccaga cagtcaggcg gtcgaaattg ctggccataa agttgatgta ttcttccgtc
    20821 agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc cgtctggcaa cagatgacca
    20881 tgccaggctc cgagatcgaa aaacggatta aaacgctggt gatcgtcgta atcgtaatcc
    20941 ttcatgtact gcggcgcacc agtacggtta atcacgtttt tataattgtc ggcgttagcg
    21001 gcatgagcgg aaaaacttat cagcagagcg caggttactg gcgttaaaat agttttgatt
    21061 ttcatgagat tacagtcctt attaccagta gaaatattgc atcaggaaga tttggtcgct
    21121 gggatcgtta gaggcatttt tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc
    21181 gccgccatat tcgtaccaga taccaaactg catgaaagag gtggtttcat cgtcagcgtg
    21241 gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga taaacgcctt ttaacgcttc
    21301 atacatggcg ctcaccatca tcccgctttc acttcccggt acgtccagcc cgtcggcacg
    21361 tccggtatgg tgccaggcgc gagcggcaaa gttaggggta aacaggcccg tgagatagag
    21421 ttgtcctgtg ccgtcggtaa tttccagacc attcatccag gtgatgcctt cggcaaggtc
    21481 atactggatg taaccgttca tccattgccg gataggtgta tttgtcgtcg tagcgatcgt
    21541 acttgccaaa atgtagccag gctttgcttt cgtcatgatg tccggcaggt gtggcggtga
    21601 cgctataacg aaagttgccg cttaagttct gtaccttcag cgcggtcata atgtcgcggg
    21661 tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc ccaccattgc aggtcatcca
    21721 gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc atcattggta cgataaccgc
    21781 catagaaacg gttaatccca ccttcgaaac caccccagct atggtcaggc acctaggcat
    21841 taccgtcgtg gtcggcctgc acggtccagc cttcgccgta aatcaggccg aaccaggagc
    21901 cgtacttcat ctccaggcca ccttcaatat aggtgccgtc gctgtattta tgtttattct
    21961 caccctctaa atccatatat ccaccaagcc ctaattcgcc gtaaaaacga aatgaaggag
    22021 aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg ttctgccgga cgtgcttctt
    22081 ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag taatgctctg catggtgcag
    22141 tgattaattt cattgtgtta atccatgagc taaagaaaaa gtacagcctt ttatcgttgt
    22201 aattataaaa atataaaggc agaaactatt ttccctctcc tcatccagga agagggaaaa
    22261 tgcctgctca ttttttatta gtctgcttaa tcaacattgc tggctggcgt cgcttcaata
    22321 tggcgctctt cagccgttaa tccctttata tatttgctgt atttccacca cgcaaagccg
    22381 aggaagataa caataccgcc gacgttataa aaaatgatgg tcagaatatt cgcgccagtc
    22441 gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa tcagcattga aacaacgata
    22501 ataccggtgc ggcgggagcc catacggaaa tcgcgtggca ggtgatctaa tttggcgcgt
    22561 aaattcaggt aagccagcat gatgaataac ggcggaagca tggacgctgc ggcggtcata
    22621 ttaataatgg tattcatcag atcctgcacg gtattggaac cgagcatcgg gataatcatc
    22681 agcgggatga cgatcagaaa ctggatccat gctgcgcgcg ccggaacgcc gttttcgtta
    22741 agttcgacgg ttttcttacc aaagatgcct tccgggattt cggagaagaa aattttcacc
    22801 ggcgttgcgg tccacatcag cagggaaccg aacattgcgg taaaggacac cagaccgaca
    22861 aagcgattca tcaacgcttc cggcagacca aaatacgccg ccatgccgtg gaatacctgc
    22921 accgatccgc cggtaaattt caactctttg ctgctgacga agacgttaat cagcaccgag
    22981 gagacggaat acagtacgcc gataaaaatc ccggcgagga tgatcacttt aacgaacgat
    23041 ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg actccgcgcc acctgcggcc
    23101 ataaagatcc aggtggtaac gccgaggaac gcccagttga agttcgggat catcgcgtca
    23161 acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg ccgtacctgc cagtaaaatg
    23221 taggagagcg ttaacagcag catcagcgtt gaagtgacgg aggtaattgg ccccagcatt
    23281 ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca gcaccatact gataacggtg
    23341 gtggcaaccg gcgtcataat atattcgtag ccgaggaagg catacgaagc ataagcaata
    23401 acgcgcggca acagcgaggt gaaaaagaac agatttacga accagtaagt gtaggcagta
    23461 ataaatgccc aacgtccgcc cagcgaactt tttacccacg cgtagacacc ggcttctgag
    23521 tttttattta acgaaacaaa ttctgcgatg atcagacaga agggaataaa ataaaaaatc
    23581 gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct caatattatt attgataacg
    23641 ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca agccgaattt gccgattgta
    23701 ttacgtttgg tatcagacat aacacatctc cgaaaatgtg tagggtagcg gtggcggcaa
    23761 actccttttg ccgccgtagt tgttatttgt tatggaaata accatcttcg atggtgactt
    23821 tgagaaccac ttttttgacc gcgttattgc agataaaccg atacgcttca tggatatcgc
    23881 aaataacgat ttgcccttcg tggacctcta cggtttctcc gcagcctttt aaatattcac
    23941 ggtcagtttc atcacgataa tattccacta cctgtaatgt ctctttcggc gcatattcaa
    24001 ttttttgctg cccttgcagg tagtaatgca cttcaaaata gcgtcgatgc ccggtaaata
    24061 gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa tgaatcaccg atggagtggg
    24121 cgacgccagg ctgaatgttg tcgatatttt caatcgcttc aacgcagcgt tgccacttct
    24181 tgccagaggc gtaaatctgg cggaactgtt ctaagttatc gatgatcctc attgcttatt
    24241 ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac tcatacgacg ccaggctttg
    24301 cgcggtagct tctccgccag aaaccggcag caacgtaaag ccgtagctga agtcacggaa
    24361 ccagacgcgc caggagtcca gcacctcgct gccccaggag ttggagccga ggccaagtag
    24421 ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc agctcgttac agtgctgggc
    24481 agcgtggatg ttttcctggg tatagtgcca ggcgctgaag ttaattgggc gctgcggaac
    24541 caccagcaga ccgttgccgt ggcggttagt cagtgccgtc cagcggacat gctgacggtt
    24601 gccgttgttc tgcgggaagg gatagttctc gaacatggca tcgacggtgc tgcgccagat
    24661 atcgatgatg ttagcctgct ggctgtcggc gtagttttcg cccggtccac gaccgtaata
    24721 cgccacctga tcgtattcgc cgttaatccc catggtgaaa ccgatgcacg gaatgatgtg
    24781 cggatagtcg ccgtaacgct cgccggaaag cgccacgtta acctggccat cggcagcgat
    24841 gcgccagatg taggtacagc gcatcccgaa gtcaaacacc ggcggtgcaa taactgtgcg
    24901 gctgatgatc aacacttcac catcgctctg ttctacggca aagtcgcgca gatgttcctg
    24961 catgatctgc aaatgattcg gctgccacaa cccttcgtac tcctgcttgt ggttgtcgat
    25021 catcggcttg aagaagttga tctttggctc gcgggtcagc agcgactcgc catttacctg
    25081 gcaggatgtc ggtttgccac tcatttttga gaaggtgatc gcgaagttgt agccgcgaac
    25141 ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc gcattatttg gtgcgaaagg
    25201 cactggctgc gcggtgtttt ccttcagcgg gaactgataa gaggcgatag aatgcccggc
    25261 ttcgctgtag cgggtgcggg aatctttggt caccgtaatg ttgaggaacg cttcgcgggc
    25321 gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg ttcggcgcaa cgtcgcgcag
    25381 tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc acctctgcgt gcagggtgta
    25441 gtcatcaagc gtggtaaacc acagtttatt ttcgactttc aactcgccgc gagtcagcgc
    25501 gtggattttt accggcgcga taacctgttt gtactctttc aggcccgatc ccggcgtctg
    25561 atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt cgccgccgaa
    25621 tttataccag acattgccgt ggtcgtcctg tgcctggatc ccgtggtcgc accactccca
    25681 gacataatga ccctgaatgc aatcgtgctt atagaagacg ttctggtact ccgtcagccc
    25741 gcccggtccg ttccccatcg catgagcata ttcacagatg atgcgcggct tcggatgcgg
    25801 gtattcacca aactcattca tcagcggcac gcgggtgtac atggtggaaa taatatcgac
    25861 cacttcagca tcgcgatctt cttcgtaatg aaccagtcgc gtgtcatcca gcgctttcgc
    25921 cgcatggtac atcgcgcgga tgttacagcc atagccggat tcattgccca gcgaccagat
    25981 gatgatcgac ggatggtttt tctgtgcgtg gatatggcga acaatgcgct cgacgtagac
    26041 tttttcccac tgcggatcgt cggtaatacg gctgatatcg ccgacattag caaagccgtg
    26101 cgattcgacg tcggtttccg ccatcacaaa caggccgtag atatcacaca gttcgtaaaa
    26161 acgcggatcg ttcgggtagt gagcggtacg cacggagttg atattgtgct gcttcatcaa
    26221 ctggagatct ttctcgacgc gatccattcc aacggcgcgg cctttgcgat gatcgttgtc
    26281 gtgacggttg acgccgtgca gcatcacata acggttattg atccagaaca gaccgtcgcg
    26341 cactttgata tcacggaagc caacgcgttg tggcaccact tccagaacgt tgccgttggc
    26401 gtctttcagc gtcatgacca gatggtaaag ataaggggat tctgctgacc attgctgcgg
    26461 ctgttcgaca gtaaaagcaa agctggcgct ggtcagtttt tcaattgcca aatgatcaat
    26521 ggcgctgctg tgcaccacgc gttcgccatc aaacagggta tattccagcg tcgtgacgac
    26581 aggggaggcg gcgagatttt ccagcaccac ttcgcaggaa agcgtggcat cgcaataggc
    26641 ttcgtcaaag tcggtacgca cagtgaaatc gttaatatgc gttaggtgtt ttccgaccag
    26701 ataaacatcg cggaagatcc ccgccgacca ccacatatcc tggtcttcca cgtaggtaga
    26761 gtccgcccac tgcatcacgc gcacacacaa caggttgtcg ccggttttaa ccatcgcgct
    26821 gatgtcaaac tctgcggtca ggcgactgcc cttgctgaaa cccacatact gaccgttaac
    26881 gtagacttca aaataggttt cgacgccgtc aaatttaatc agcgtctgtt taccctgcca
    26941 gccgtcgctg agggtgaaaa tacgttgata ggcaccggtt gggttatcgc tggggacaaa
    27001 cggcacatcg atggggaacg gaaaaccttc gtcggtatat tgcagtttgc cgtgaccttc
    27061 catttgccac atggcgggga cggtaatatg cccccagtca gccattaact cagaggtgaa
    27121 ggcttctggt acttgcagcg gatggtcaaa aaagtggaaa ttccactgac tgcttaaggg
    27181 cagaaacagg ctgctggttt cgcgggcaaa ggtacgcgct tgcgcaacag aatcatatga
    27241 aaaaaagtac gcacgcggcg caagtcggtt ttcgtgggtg agctgaatgt tttcccagcg
    27301 attcataggg cctcccggta gagagaaatc ggaaccattc atggtgatgg tagtttgtgt
    27361 aattgctggg gggttagtaa aatttttact aaaaagcagc aggtaaatgc atttacttta
    27421 actggatcac gaaaataaac ggcagcactg tccggcaaaa atgattgccg gacagtgtgt
    27481 aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactagcagc
    27541 ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc
    27601 atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata
    27661 tcgttaacgc tgataagcga aatatcttgt gggatgttca ggccacgttc atgaattgcc
    27721 cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc
    27781 cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag
    27841 atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga
    27901 atatccgcct tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg
    27961 ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg
    28021 taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca
    28081 gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg
    28141 tctggtaatc cgttgtgttc ataacagttg gtcagttcga tcgccagctt ttcgcactga
    28201 gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg
    28261 tagctgtaga tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg
    28321 ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct
    28381 ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca
    28441 gcttcgattg cgatgtcttt taatgttgcc atgtttacct tttttacttt ccctgatgtg
    28501 cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt
    28561 tctttgacgc tgtaacggta acacagcggc ttaggaatgt gatcacgcct gcattctcca
    28621 tttttagcct acaggattga gaaaaacttt tctatagttt agtaaaattt tatccgaagg
    28681 gaggaagcaa tggaaaccgt ggcttacgct gattttgcac gtctggaaat gcgcgtcgga
    28741 aagattgtgg aagtgaaacg ccatgaaaac gccgacaagc tgtacatcgt acaggttgat
    28801 gtggggaaaa aacactgcaa accgtgacca gcctggtgcc gtactacagc gaagaggagc
    28861 tgatggggaa aacggtggtg gtcttatgca atctgcaaaa agcgaaaatg cgtggtgaaa
    28921 cgtcggaatg catgctgttg tgtgcggaaa ccgatgacgg cagtgaaagc gtgttgttaa
    28981 cgccggagcg gatgatgcct gcgggcgtac gcgtcgtata aaaagatcgg atggcgacgt
    29041 cgtatcgcca tccgatttga tattacgctt cttcgacact tactcgcatg gccgccagcg
    29101 ccttacgcgc cgctttgatc accagttcac actgttcaat ggtcagtgtc agtggcggtt
    29161 caatgcggat cgttttggcg ttattgagcg ttccggccac cagtacgcgc tggcggaaca
    29221 tctcgctggc aaagttatag ccgatttcgt tatcaacaaa ctcaatcgcc atcaacatcc
    29281 ctttaccacg cgcttcctgt accagatcgg gatattcccg cgccagttga cggaaaccgt
    29341 ccagcaacat atcgcctttt tgctcagcct gagccggtaa gttctgctcc agcaacacat
    29401 tgatggtcgc cagcgccgcc gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca
    29461 ggaatgggtt gtcgaacaga actgaaaaca cctcttcagt ggcgatggtc gcgccaatcg
    29521 gcatcacgcc gccgccgagg gcttttgcca gacacagaat gtccggctga acgttctcat
    29581 gttcgcaggc aaacatcttg cccgtgcgcc ccatgcccgt ttgtacttca tcgaggatca
    29641 tcagtgcgcc gaactcatcg cacagcttac gcacggcggt gagatagccc ggcggcggca
    29701 gaattacgcc accttcgccc tgaatcggtt cgaggatcac cgcagccaca tcatcaccgg
    29761 tttttttgca ctcattaaga gccgtgcgca tggcttcgat attgccaaac ggcacatgac
    29821 ggaaccctgg cagcagtggc ataaacggtt tgcggaaggt cgatttcgcc gtggccgaca
    29881 gcgcgccaag tgatttaccg tggaacgcgc cgctggtggc aataaaagta aatttgccgc
    29941 gcggcgactg ataagccttc gccagcttca gcgccgcttc aacggactcg gtgccgctat
    30001 tacagaagaa gctgtatttc agtttaccgg gcgttagcgc agcaagggtt ttcgccagca
    30061 tagcccgtaa cggatcgagt agctcctggc tgtgcagtgg ttgtttcgca agttgattct
    30121 gtacggcgga aaccacaact ggattacggt gccccacgtt gaaaattcca aaacctccca
    30181 ggcagtcgat aaactcctgt ccctgggtgt cgacaagcgt atttaaacct cccgcttgcc
    30241 actctacggc tccgtaatcc ccgccggcgg taacagattt gcgatactct aaaaaccccg
    30301 gattgacatg ctctttgaag tattcaatca cctctcggtt aagtgctttc atctcctcat
    30361 gatccagcgt tcgcttctca atgagattca gggcgtgggc gctgcacgct aaagccgatg
    30421 cgctcgaagg taacctgttc aaaatatgct ccggaggctc gcgtatcaca tgataccgat
    30481 ttaagtattg cagggattgc gccactccgg ctgcgatcgc gaaaatcggg ataaacacaa
    30541 atgcggttat catttgtgca atatttaatg ctatccgtta acatttgttg ttattttgca
    30601 cggtttttgt cgggtatgga tttgatgatt gctgcgcaaa tgcacgtttt cagggcaata
    30661 attgcctgat ttttgggcaa gataattaac gttttcgtat agtaacagag tgaaagtatt
    30721 ttaaatcaaa gaattagatt tgtgctttaa tcgcaaattg cgatctaaat caaattaatc
    30781 ggttaaagat aaccgcagcg gggccgacat aaactctgac aagaagttaa caaccatata
    30841 acctgcacag gacgcgaaca tgtcttctca tccgtatgtc acccagcaaa ataccccgct
    30901 ggcggacgat accactctga tgtccactac cgatctgcaa agctatatca ctcatgctaa
    30961 tgacactttt gtgcaggtga gcggctttac cttgcaagag ttacaagggc agccgcacaa
    31021 catggtgcgt cacccggata tgccaaaagc ggcgtttgcg gatatgtggt tcaccctgaa
    31081 aaaaggggag ccctggagcg gcatcgtgaa aaatcgccgc aaaaatggcg accattattg
    31141 ggtgcgggcc aatgcggtac cgatggtgcg cgagggaaaa atcagtggct atatgtcgat
    31201 tcgtacccgg gcgacggatg aagagattgc ggcggtggag ccgctgtaca aagcgttgaa
    31261 ctccggacgt accagtaagc gtattcataa aggcctggtg gtgcgtaaag gctggctggg
    31321 taaactgcct tcattaccgc ttcgctggcg gacgcgtgga gtgatgaccc tgatgtttat
    31381 cttgctggcg gccatgcttt ggtttgttgc tgccccggtg gtgacgtatt tcctctgtgt
    31441 gttagtggta ttgttggcaa gcgcttgttt tgaatggcag attgtgcgcc cgatagaaaa
    31501 tgtcgcccgt caggcactga aggtggcgac cggagagcgt aatagtgttg agcacctgaa
    31561 tcgcagcgat gagctggggc tgacattacg cgcggtaggg cagcttggcc tgatgtgccg
    31621 ttggctaatt aacgatgtct caagccaggt gtccagtgtc agaaatggca gtgagacgct
    31681 ggcgaaaggc accgatgaac tgaacgaaca tacccagcag acagttgata acgttcagca
    31741 aacggtggcg accatgaacc aaatggcggc gtcggtgaaa cagaactctg ccacggcgtc
    31801 ggctgccgat aaactttcta tcaccgccag taatgcggca gtgcagggtg gggaggcgat
    31861 gaccacggtg atcaagacaa tggacgatat cgccgacagt acctagcgca ttggcaccat
    31921 tacttcgctg attaacgata ttgcgtttca gaccaatatt ctggccctga atgcggcggt
    31981 ggaagcggcg cgtgccggcg aacagggcaa aggttttgca gtggtggcag gggaagtgcg
    32041 tcatttagcc agccgcagcg ctaatgctgc caacgatatt cgcaagctga ttgatgccag
    32101 tgctgataag gtgcaatccg gttcgcagca ggtacacgcc gccggacgga cgatggaaga
    32161 tattgtggca caggtgaaaa acgtcaccca gttgatcgcc cagattagcc attcaacgct
    32221 ggaacaggcc gatgggcttt ccagcctgac ccgtgcagtg gatgagctta acctgatcac
    32281 ccagaaaaat gccgagctgg tggaagagag tgcgcaggtg tcggcgatgg tgaaacaccg
    32341 cgccagccga ctggaagacg cggtgacggt gctgcattaa tcggtatgcc ggatccggcg
    32401 gttggagcac aatgcctgat gcgatgctgg cgcatcttat caggcctgcg gggtgtgtag
    32461 cgggtgtagg ccggataagg cgcgtcaagc gtcgcatccg gcaattgcac cgcgccactg
    32521 gcggatgcgg cgattaatcc agttgcgtaa tatcaaaagc ggcgcggtcg atcaccgcaa
    32581 tgatcttttt tatttgtgca tcactgatat cgctctggtt gacgcgtaaa tccagcaccg
    32641 ctttaaaatt atccagcgcc cgcttcattt gcgggttctg gcgcagcgcc gcgccaacgc
    32701 aacgcgcttt gatgcgttct tcaatcatct ccacttgttc gcggttttct tccagccact
    32761 gcgcaccttg ttcggtcagc gcaatctgct tcttaccacc ttcctcttcg cggatggtaa
    32821 tcagcgactg ctcctgcaga aaatccagcg tcgggtagat gacgcccggg cttggggtgt
    32881 aattcccctg ggttagattc tcaatcgctt taatcaattc gtaaccgtgg ctgtcatcgc
    32941 gcgagagaat atccagaatc accagacgca attcaccgtg accaaagaaa cgttgccgac
    33001 gaccgccgcc gcgaccgtgg cgaccaccgc aacattgacc gtgttcatgc tggtgaccgt
    33061 gtccgcagtg ctcgtgttct gacttctcac ctttgcagca gccctcatgc cgtggctggc
    33121 cttcatgttt acaacaccct tcgtgatgat ggctcatatc agcctccttt tgcttgatta
    33181 gatatatcta atttatatct gatgtgagca ttactgcaag tacaaaaatg atttttaaac
    33241 gtggaagcac ctcacatcta ggccggataa gacgttcacg ccgcatccgg cagcattgct
    33301 atggcactga atgccttcac atacttcaga catatcagtt ctacaaatcg cttgcattta
    33361 tcatgattaa caatcattat catttgcgaa ttttatttag atatatctga ttaccatcac
    33421 gaaggcgata acaatgaata acaccccccg ctacccgcag cgcgttcgca atgatctgcg
    33481 cttccgtgaa ctgactgtgc tacgcgttga gcgcatcagc gccggttttc agcgcattgt
    33541 ccttggcggc gaggcgttgg acggttttac atcgcgcggc tttgacgatc acagcaaact
    33601 cttctttcct caacctgacg ctcactttgt gccgccaacg gtaacggaag agagcatcgt
    33661 ctggccggaa ggaccacgcc caccgtcgcg tgactatacg ccactgtatg acgaactacg
    33721 ccatgaactg gcgatcgatt tctttattca cgacggtggg gtcgccagcg gctgggcgat
    33781 aaacttacgg tggcaggtcc gcgcggttcg ctggtggtgc cggaagatta cgcgtatcag
    33841 ctgtatgtct gcgatgaatc cggaatgccc gcactgcgcc gccgcctgga aacgttgagc
    33901 aaacttgccg ttaaaccgca agttagtgcg ctggttagcg tgcgggataa cgcctgtcag
    33961 gattatctcg cgcaccttga tggttttaat atcgaatggc tggcacatga tgagcaggcg
    34021 gtagatgcgc gtctggcgca gatgcaaatc cctgccgatg attacttcat ctggataacc
    34081 ggcgaaggta aagtcgttaa gaatttaagc cgccgctttg aagcggaaca gtatgaccca
    34141 cagcgggtac gtgcagcggc ttactggcac gcaaagtaac tgatatataa aaagaaaaag
    34201 gctgacgatt tctcgtcagc ctttgctata tctggtggcc cctgctggac ttgaaccagc
    34261 gaccaagcga ttatgagtcg cctgctctaa ccactgagct aaggggccat ggtagcggat
    34321 tataaagtaa ctccgtgtcg caatccagcc attaccgcgc gcctgctgtt tttataaaca
    34381 atgtattttc aatccgttat acttttcctg gtgatgttag aaaggagtaa atatggttga
    34441 ggatattttg gctccagggt tacgggtcgt gttttgcggt atcaaccctg ggctttcatc
    34501 cgccgggact ggttttccct ttgctcatcc ggcaaatcgc ttctggaagg tgatatatca
    34561 ggccgggttt accgaccgtc agttgaagcc gcaggaggca cagcatctgc tggattatcg
    34621 ttgtggcatc accaaactgg tagaccgtcc aacggtgcaa gccaatgaag tttcaaagca
    34681 ggagctgcac gcaggtgggc gtaagctgat tgaaaaaatt gaagattatc agccgcaggc
    34741 gttggcgatt ctgggcaaac aagcatatga acagggattc agctagcgcg gtgcacagtg
    34801 ggggaaacaa acgctcacca ttggttcgac gcagatttgg gtgctgccaa atcccagcgg
    34861 tttaagtcgc gtttcactgg agaaactggt tgaagcgtat cgcgagctgg accaggcgct
    34921 ggtagtgcgt gggcgataaa aaacgtcacc gg
//
LOCUS       DAJTNW010000035        34466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNW010000035 DAJTNW010000000
VERSION     DAJTNW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..332)
                     /locus_tag="OO179_001802"
     CDS             complement(<1..332)
                     /locus_tag="OO179_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531893.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO179_001803"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO179_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5531894.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO179_001804"
     CDS             complement(1693..2073)
                     /locus_tag="OO179_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5531895.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO179_001805"
     CDS             complement(2087..2503)
                     /locus_tag="OO179_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531896.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO179_001806"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO179_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5531897.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO179_001807"
     CDS             complement(3504..3971)
                     /locus_tag="OO179_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5531898.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO179_001808"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO179_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5531899.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO179_001809"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO179_001809"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5531900.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO179_001810"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO179_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5531901.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO179_001811"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO179_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5531902.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO179_001812"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO179_001812"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5531903.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO179_001813"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO179_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5531904.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO179_001814"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO179_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5531905.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO179_001815"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO179_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5531906.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO179_001816"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO179_001816"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5531907.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO179_001817"
     CDS             complement(14626..15441)
                     /locus_tag="OO179_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5531908.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO179_001818"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO179_001818"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5531909.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO179_001819"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO179_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5531910.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO179_001820"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO179_001820"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5531911.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO179_001821"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO179_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5531912.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO179_001822"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO179_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5531913.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO179_001823"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO179_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5531914.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO179_001824"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO179_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5531915.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO179_001825"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO179_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5531916.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO179_001826"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO179_001826"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5531917.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO179_001827"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO179_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5531918.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO179_001828"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO179_001828"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5531919.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO179_001829"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO179_001829"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5531920.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO179_001830"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO179_001830"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5531921.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO179_001831"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO179_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5531922.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO179_001832"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO179_001832"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5531923.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO179_001833"
     CDS             31575..32189
                     /locus_tag="OO179_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5531924.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO179_001834"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO179_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5531925.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO179_001835"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO179_001835"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5531926.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8112 a         8706 c         9313 g         8335 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgccgctg agaaaaagcg aagcgg
//
LOCUS       DAJTNW010000036        34239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNW010000036 DAJTNW010000000
VERSION     DAJTNW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..578
                     /locus_tag="OO179_001836"
     CDS             <1..578
                     /locus_tag="OO179_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5531927.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="OO179_001837"
     CDS             complement(575..1711)
                     /locus_tag="OO179_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5531928.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="OO179_001838"
     CDS             complement(1717..2040)
                     /locus_tag="OO179_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5531929.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="OO179_001839"
     CDS             2223..2366
                     /locus_tag="OO179_001839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531930.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO179_001840"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO179_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5531931.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO179_001841"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO179_001841"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5531932.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO179_001842"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO179_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5531933.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO179_001843"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO179_001843"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5531934.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="OO179_001844"
     CDS             complement(8959..9567)
                     /locus_tag="OO179_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5531935.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="OO179_001845"
     CDS             complement(9638..10723)
                     /locus_tag="OO179_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5531936.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="OO179_001846"
     CDS             complement(10726..11088)
                     /locus_tag="OO179_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5531937.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="OO179_001847"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="OO179_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5531938.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="OO179_001848"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="OO179_001848"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5531939.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="OO179_001849"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="OO179_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5531940.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="OO179_001850"
     CDS             complement(15380..15589)
                     /locus_tag="OO179_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5531941.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="OO179_001851"
     CDS             15873..16235
                     /locus_tag="OO179_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5531942.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="OO179_001852"
     CDS             16779..17462
                     /locus_tag="OO179_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5531943.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="OO179_001853"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="OO179_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="OO179_001854"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="OO179_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5531944.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="OO179_001855"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="OO179_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5531945.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="OO179_001856"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="OO179_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5531946.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="OO179_001857"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="OO179_001857"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5531947.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="OO179_001858"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="OO179_001858"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5531948.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="OO179_001859"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="OO179_001859"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5531949.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="OO179_001860"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="OO179_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5531950.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="OO179_001861"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="OO179_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5531951.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="OO179_001862"
     CDS             complement(29422..29853)
                     /locus_tag="OO179_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5531952.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="OO179_001863"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="OO179_001863"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5531953.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="OO179_001864"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="OO179_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5531954.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="OO179_001865"
     CDS             32939..33898
                     /locus_tag="OO179_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5531955.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34095)
                     /locus_tag="OO179_001866"
                     /pseudo
     CDS             complement(33885..34095)
                     /locus_tag="OO179_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462607.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(34107..>34239)
                     /locus_tag="OO179_001867"
     CDS             complement(34107..>34239)
                     /locus_tag="OO179_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5531956.1"
                     /translation="IERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         8506 a         8967 c         8723 g         8043 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcgcacc gggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg
    34141 atgaccttgt catgcagctc caccgatttt gagaacgaca gtgacttccg taccagcctt
    34201 gccagatgtt gtctcagatt cagattatgt cgctcaatg
//
LOCUS       DAJTNW010000037        33520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNW010000037 DAJTNW010000000
VERSION     DAJTNW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO179_001868"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO179_001868"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5531957.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO179_001869"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO179_001869"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5531958.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO179_001870"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO179_001870"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5531959.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO179_001871"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO179_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5531960.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO179_001872"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO179_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5531961.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO179_001873"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO179_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5531962.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO179_001874"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO179_001874"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5531963.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO179_001875"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO179_001875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5531964.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO179_001876"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO179_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5531965.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO179_001877"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO179_001877"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5531966.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO179_001878"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO179_001878"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5531967.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO179_001879"
     CDS             8212..9075
                     /locus_tag="OO179_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5531968.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO179_001880"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO179_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5531969.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO179_001881"
     CDS             10411..11028
                     /locus_tag="OO179_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5531970.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO179_001882"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO179_001882"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5531971.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO179_001883"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO179_001883"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5531972.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO179_001884"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO179_001884"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5531973.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO179_001885"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO179_001885"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5531974.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO179_001886"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO179_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5531975.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO179_001887"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO179_001887"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5531976.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO179_001888"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO179_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5531977.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO179_001889"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO179_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5531978.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO179_001890"
     CDS             21859..23568
                     /locus_tag="OO179_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5531979.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO179_001891"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO179_001891"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5531980.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO179_001892"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO179_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO179_001893"
     tRNA            27625..27719
                     /locus_tag="OO179_001893"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="OO179_001894"
     CDS             28020..29204
                     /locus_tag="OO179_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5531981.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="OO179_001895"
     CDS             29616..30659
                     /locus_tag="OO179_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5531982.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OO179_001896"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OO179_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="OO179_001897"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="OO179_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="OO179_001898"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="OO179_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8399 a         8808 c         8262 g         8051 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
//
LOCUS       DAJTNW010000038        33119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNW010000038 DAJTNW010000000
VERSION     DAJTNW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(2..94)
                     /locus_tag="OO179_001899"
     tRNA            complement(2..94)
                     /locus_tag="OO179_001899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO179_001900"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO179_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5531983.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO179_001901"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO179_001901"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5531984.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO179_001902"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO179_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5531985.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO179_001903"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO179_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5531986.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO179_001904"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO179_001904"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5531987.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO179_001905"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO179_001905"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5531988.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO179_001906"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO179_001906"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO179_001907"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO179_001907"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5531989.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO179_001908"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO179_001908"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5531990.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO179_001909"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO179_001909"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5531991.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO179_001910"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO179_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO179_001911"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO179_001911"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5531992.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO179_001912"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO179_001912"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5531993.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO179_001913"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO179_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5531994.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO179_001914"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO179_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5531995.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO179_001915"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO179_001915"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5531996.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO179_001916"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO179_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5531997.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO179_001917"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO179_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5531998.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO179_001918"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO179_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5531999.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO179_001919"
     CDS             complement(20900..21019)
                     /locus_tag="OO179_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5532000.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO179_001920"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO179_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5532001.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO179_001921"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO179_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5532002.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO179_001922"
     CDS             complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO179_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5532003.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO179_001923"
     CDS             complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO179_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5532004.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO179_001924"
     CDS             complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO179_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5532005.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26927..27277
                     /gene="hypA"
                     /locus_tag="OO179_001925"
     CDS             26927..27277
                     /gene="hypA"
                     /locus_tag="OO179_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5532006.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27281..28153
                     /gene="hypB"
                     /locus_tag="OO179_001926"
     CDS             27281..28153
                     /gene="hypB"
                     /locus_tag="OO179_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5532007.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28144..28416
                     /gene="hypC"
                     /locus_tag="OO179_001927"
     CDS             28144..28416
                     /gene="hypC"
                     /locus_tag="OO179_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5532008.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28416..29537
                     /gene="hypD"
                     /locus_tag="OO179_001928"
     CDS             28416..29537
                     /gene="hypD"
                     /locus_tag="OO179_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5532009.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29534..30544
                     /gene="hypE"
                     /locus_tag="OO179_001929"
     CDS             29534..30544
                     /gene="hypE"
                     /locus_tag="OO179_001929"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5532010.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30618..32696
                     /gene="flhA"
                     /locus_tag="OO179_001930"
     CDS             30618..32696
                     /gene="flhA"
                     /locus_tag="OO179_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5532011.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32733..33086)
                     /locus_tag="OO179_001931"
     CDS             complement(32733..33086)
                     /locus_tag="OO179_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5532012.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7802 a         9229 c         8622 g         7466 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcagg ataatgccga tattttccag ggtgtggtaa
    24661 gccagcaggc gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg
    24721 atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt
    24781 gacagggtta acatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca
    24841 gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatccca
    24901 gccagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt
    24961 tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag
    25021 caagcaatca ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct
    25081 ttgctgttgc tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac
    25141 atgccgaggt tgctggcgat gacggcgcag acggcggcag ccatcaacat attgatctgc
    25201 aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac
    25261 agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg
    25321 tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc
    25381 gcggctgccg tatacagact accaaccgcg ccgccaattc cggctatcca gccacttagc
    25441 gctttttgaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    25501 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    25561 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    25621 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    25681 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    25741 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    25801 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    25861 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    25921 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    25981 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    26041 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    26101 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    26161 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    26221 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    26281 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    26341 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    26401 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    26461 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    26521 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    26581 ggcgtgatac aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    26641 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    26701 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    26761 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    26821 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    26881 tggcgcaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    26941 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    27001 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    27061 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    27121 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    27181 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    27241 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    27301 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    27361 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    27421 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    27481 tcacacaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    27541 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    27601 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    27661 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    27721 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    27781 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    27841 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    27901 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    27961 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    28021 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    28081 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    28141 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    28201 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    28261 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    28321 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    28381 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    28441 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    28501 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    28561 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    28621 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    28681 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    28741 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    28801 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    28861 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    28921 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    28981 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    29041 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    29101 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    29161 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    29221 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    29281 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    29341 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    29401 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    29461 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    29521 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    29581 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    29641 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    29701 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    29761 gatttgcggc accgcgaatg acgttgcggg cagtggcgct attccgcgct atctctcctg
    29821 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    29881 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    29941 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    30001 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    30061 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    30121 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    30181 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    30241 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    30301 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    30361 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    30421 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    30481 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    30541 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    30601 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    30661 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    30721 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    30781 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    30841 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    30901 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    30961 ccaaatttgg tcactattgc ctgatgccac tggcggcggg agggcatatt tttggtggct
    31021 gtgaatttat tcgttatgac gagcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    31081 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    31141 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    31201 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    31261 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    31321 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    31381 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    31441 atctctacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    31501 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    31561 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    31621 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    31681 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    31741 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    31801 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggctcgggta
    31861 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    31921 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    31981 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
    32041 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    32101 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    32161 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    32221 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    32281 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    32341 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    32401 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca
    32461 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    32521 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    32581 aaaccaacag cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    32641 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    32701 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    32761 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    32821 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    32881 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    32941 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    33001 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    33061 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatt
//
LOCUS       DAJTNW010000039        32958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNW010000039 DAJTNW010000000
VERSION     DAJTNW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            15..293
                     /gene="tnpA"
                     /locus_tag="OO179_001932"
                     /pseudo
     CDS             15..293
                     /gene="tnpA"
                     /locus_tag="OO179_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(311..979)
                     /locus_tag="OO179_001933"
     CDS             complement(311..979)
                     /locus_tag="OO179_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5532013.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1282..1875)
                     /gene="tehB"
                     /locus_tag="OO179_001934"
     CDS             complement(1282..1875)
                     /gene="tehB"
                     /locus_tag="OO179_001934"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5532014.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1872..2864)
                     /gene="tehA"
                     /locus_tag="OO179_001935"
     CDS             complement(1872..2864)
                     /gene="tehA"
                     /locus_tag="OO179_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5532015.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2988..3968
                     /locus_tag="OO179_001936"
     CDS             2988..3968
                     /locus_tag="OO179_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532016.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(3963..4499)
                     /gene="rimL"
                     /locus_tag="OO179_001937"
     CDS             complement(3963..4499)
                     /gene="rimL"
                     /locus_tag="OO179_001937"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5532017.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4562..4786)
                     /locus_tag="OO179_001938"
     CDS             complement(4562..4786)
                     /locus_tag="OO179_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5532018.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4926..6581)
                     /gene="opgD"
                     /locus_tag="OO179_001939"
                     /gene_synonym="mdoD"
     CDS             complement(4926..6581)
                     /gene="opgD"
                     /locus_tag="OO179_001939"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5532019.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6806..8149)
                     /locus_tag="OO179_001940"
     CDS             complement(6806..8149)
                     /locus_tag="OO179_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5532020.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8366..9289
                     /locus_tag="OO179_001941"
     CDS             8366..9289
                     /locus_tag="OO179_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532021.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9327..10967)
                     /gene="trg"
                     /locus_tag="OO179_001942"
     CDS             complement(9327..10967)
                     /gene="trg"
                     /locus_tag="OO179_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5532022.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11343..12712
                     /locus_tag="OO179_001943"
     CDS             join(11343..11823,11823..12712)
                     /locus_tag="OO179_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5532023.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            12814..12966
                     /gene="hokA"
                     /locus_tag="OO179_001944"
     CDS             12814..12966
                     /gene="hokA"
                     /locus_tag="OO179_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5532024.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(13153..13365)
                     /gene="cspA"
                     /locus_tag="OO179_001945"
     CDS             complement(13153..13365)
                     /gene="cspA"
                     /locus_tag="OO179_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5532025.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(13646..13936)
                     /locus_tag="OO179_001946"
                     /pseudo
     CDS             complement(13646..13936)
                     /locus_tag="OO179_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            14370..15080
                     /locus_tag="OO179_001947"
     CDS             14370..15080
                     /locus_tag="OO179_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5532026.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(15130..16104)
                     /gene="ghrB"
                     /locus_tag="OO179_001948"
     CDS             complement(15130..16104)
                     /gene="ghrB"
                     /locus_tag="OO179_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5532027.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(16208..16858)
                     /locus_tag="OO179_001949"
     CDS             complement(16208..16858)
                     /locus_tag="OO179_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5532028.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            17021..19354
                     /gene="bisC"
                     /locus_tag="OO179_001950"
     CDS             17021..19354
                     /gene="bisC"
                     /locus_tag="OO179_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5532029.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYV
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(19323..19763)
                     /locus_tag="OO179_001951"
     CDS             complement(19323..19763)
                     /locus_tag="OO179_001951"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5532030.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(19760..20323)
                     /gene="tag"
                     /locus_tag="OO179_001952"
     CDS             complement(19760..20323)
                     /gene="tag"
                     /locus_tag="OO179_001952"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5532031.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            20481..21179
                     /locus_tag="OO179_001953"
     CDS             20481..21179
                     /locus_tag="OO179_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5532032.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            21408..22616
                     /locus_tag="OO179_001954"
     CDS             21408..22616
                     /locus_tag="OO179_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532033.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            22940..24631
                     /gene="eptB"
                     /locus_tag="OO179_001955"
     CDS             22940..24631
                     /gene="eptB"
                     /locus_tag="OO179_001955"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5532034.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            24723..24799
                     /locus_tag="OO179_001956"
     tRNA            24723..24799
                     /locus_tag="OO179_001956"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24757..24759,aa:Pro,seq:cgg)
     gene            25543..27150
                     /gene="dppA"
                     /locus_tag="OO179_001957"
     CDS             25543..27150
                     /gene="dppA"
                     /locus_tag="OO179_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5532035.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            27458..28477
                     /gene="dppB"
                     /locus_tag="OO179_001958"
     CDS             27458..28477
                     /gene="dppB"
                     /locus_tag="OO179_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5532036.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            28487..29389
                     /gene="dppC"
                     /locus_tag="OO179_001959"
     CDS             28487..29389
                     /gene="dppC"
                     /locus_tag="OO179_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5532037.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            29400..30383
                     /gene="dppD"
                     /locus_tag="OO179_001960"
     CDS             29400..30383
                     /gene="dppD"
                     /locus_tag="OO179_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5532038.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            30380..31384
                     /gene="dppF"
                     /locus_tag="OO179_001961"
     CDS             30380..31384
                     /gene="dppF"
                     /locus_tag="OO179_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5532039.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(31414..32685)
                     /locus_tag="OO179_001962"
     CDS             complement(31414..32685)
                     /locus_tag="OO179_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5532040.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         8182 a         8143 c         8289 g         8344 t
ORIGIN      
        1 tgttaattaa ctaacctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggcg
       61 tatcgggtag gttactgcga cgagatcgac cagatattgc agtcaggtat tactacaaag
      121 gcgttttgtg gagtcctggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca
      181 tccgccaata cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc gccttatatc
      241 cccgccctga aggatggggt tttacggcgc accggataaa aagcgccatc taacgatggc
      301 gcgtctggaa ctactttttg ttaacgtcaa acatggtggc atcggtcgcc atgtcatcaa
      361 taacttgttt aatattttcg aaagccattg gcgtactttc gttattcagg tctttacctt
      421 cgccctgacg aacgactttg ataaccggtt tattagtcgc tgcatcaatc agctcacctt
      481 caaaatagag gcgagtatcc atggtacggt ggcctgtagc catttgcgtc cccgcaacca
      541 ctaatgcaac aggaaccact tcatagaatt gcagcccttc tttgctggta tctacaccgg
      601 taatggcccc acggaaaatc agactacgcg gcccagcggt ggtaaccact ggtttacgct
      661 gcgctatcgc ttctttcatt tcggtgttgg tatagttcaa aattttatcc agaactttct
      721 gccctacctg ggtagaaggt tttggtaccg gataataagt gattgggttc cagacgatgc
      781 tgtcatattt gctttgatca aaactcgggt ctacccaacg taaaacaggt ttacctgttg
      841 ctgaggttgt ttcttttaaa tcagagtaat tgtttaaaaa accagaatat ttatctggct
      901 gggtgatttt agaggcacaa ccagacagag ccaacaagcc gcataaagct gcaactttcg
      961 caaatgatgt ggtacgcatg aatagtttcc tgagatgatt atattacttt ctaagttata
     1021 gcaaaaagcg ccgcttcgtg ttgtggcaaa aaaagtgttg gtgaaaataa atttttcagc
     1081 cccttttcag acgacatagt caatgccgaa aaaggggcta agtcaatcat cagtagttaa
     1141 atttcagtaa gttaagctgc taaaaaactt cgactactgt gattaatttc acatcccttc
     1201 gataaaaaat atgcttaccg aaaagaagaa cagtaaatca tctcagcgtg gggaattttc
     1261 acgcgttttg tgcttaccgg gtcatttttt acgtgccagc atcgtggcga aacgcagttt
     1321 aatacgatta ccgttggcgt cggtgcggtg cagctcgccg acgtcttcat tgtatttcac
     1381 cctctcccag ccttcgtaat atcgacgtaa ttctccctct ttgaaggcaa acgtgaagcc
     1441 gacggtacat ggataatcag cggtatccat cgccgccaca atcaggttgt aaccaccagg
     1501 tttagtgcaa cgttgcatat tggcaatcaa ccctgggatg gttttagcct caaggaacat
     1561 cagcaccaca gtcgaaagaa taaaatcgta ctgtccatca aatgtgaggt tattcagatc
     1621 aacgacccgg gtgtgtaaat tatccagatt ttcaatggat ttaatgcgct cgacgttggc
     1681 gatactcatg gcatttttat cccatgcgtc aacatcataa ccattggctg ccaggtaaag
     1741 actgttacga ccattgccac agcccagatc cagcgtttta cccggtttaa ccactttcac
     1801 cgcttccagt acttcagagt gtgtgcgggt taattcatat ttatcagtaa aatagttttc
     1861 gtcacgaatg atcattcttt gtcctctgct ttcattaaaa cggcgcgctc ggttctgact
     1921 aacaattttc cctgcatcag aagcgcaaaa gtacggatga gcagtattgc aataataaaa
     1981 ttggtaaaga taaacagcgg caccgccagc gtatggaaaa atccattatc gctgccactc
     2041 cccagatgca aaccggtggt tgccagtgca gatacgccga acgagaaact ccagaatgaa
     2101 gcattaaatg gctgggagag ataccatggc atcagacgta gcataaacag cagttgcagc
     2161 agtccataac cgaaaagcat tttcgccagc gtgtcaccct cgccgccgtt gacgctcagc
     2221 caggcactac aggccaccag cgcaggagcg agctgaatgc cgagtgatgt ccgcagtgcc
     2281 gtgggtaatt ctcccgaact gcgcagacgc tgcaagatca ccggttcaag gcttagccat
     2341 gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg tgtagcccaa cgcaccacag
     2401 gccattgcgc tgataaagtt gttggcaact gtcggcagat acagtccagg cgtggtagct
     2461 tcttcagggt gagatccgcg ccataatccc gcagtttgcc aggcggcata agccaactga
     2521 accacgacac caaaactgaa catgcacacc gccagtgggc gaaaccacgg aacaaaacca
     2581 atcgccacca gcatcgtcgt tgccggaaac aaactcacaa aactgctcag cactggatgg
     2641 cgaacttccg ccagcacgct atgcggaaag cgtatgagtc gggtaataaa tgcgctagtc
     2701 aataatcccc agatgatcat cgccagaatc accagcccat cccctaacca gtggctgacc
     2761 tgccaaacct ggctggcata gcgccaggca aatcccatcc ctatcgtccc caacacaata
     2821 ccaaagtagc ctgccggcaa attgagcact ttatcgctct gcatcttctt actcattttg
     2881 tttaatttat aaagtatatt tgaaatgcat tttatggaat tttctcgagc atagccagag
     2941 ccgcagaatt tgctacggtt atagtaagtc acctgtggga aaacgctatg cgcaaatatc
     3001 gcctaagtga agaacagcga gcctttagtt atcaggaaga tggcactaaa aaaagtgtgt
     3061 tattacggca gattatcgcc attagcgatt ttaacgatgt gatagcagga accgctggcg
     3121 gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa ttgctggatt tacgaccaga
     3181 atgctattgc gttcggcgga acggtgattt ccggtaatac gcgtatcacc ggcaccagcg
     3241 tgctgtgggg agaggtttac gcaacggata acgtctggat cgataatagt gaaatcagcc
     3301 agggtgcata tataagtgac agtgtcacca ttcatgactc attagtttac gtgcaatgta
     3361 gaatatttgg tcacgcctta attgatcaac attctatgat cgtcgccgca caaggcttaa
     3421 cgcccgacca tcaactcctg ctccaaattt atgatcgcgc aagggttagc gcctcgcgta
     3481 ttgtccacca ggcacaaatt tatggcgatg ccgtcgtccg gtatgctttt attgaacatc
     3541 gcgccgaagt ttttgatttt gccagtgttg aaggtaatga agagaacaac gtctggctat
     3601 gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc tggcatagaa gaagatgcca
     3661 ttccgacgat tcattacagt tcgcaggtgg cggaatatgc cattgtggaa ggtaattgtg
     3721 tgttaaaaca tcatgtcttg attggcggaa atgccgtggt acgtggtgga ccgattctgc
     3781 tcgacgagca cgttgtaata caaggtgaga gccgtattac aggtgcggtg ataattgaaa
     3841 accatgtgga gttaaccgac cacgccgtcg ttgaagcctt tgatggcgac acggttcatg
     3901 tccgtggtcc aaaagtcatt aacggcgagg agcgcattac gcgaacgccg ctggcagggt
     3961 tattatgaat cgataatacg cgcgtataag ttcacatcat cataggcatc attcaggaac
     4021 tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc gcaaagcgac ctggttgctt
     4081 tgcggattgt ccacccgaca tttgatcacg aagcgtctaa gttcaccaga ctgagcgtaa
     4141 tgatgaatca atgcctgcag cgcctgagaa atgatcccct gcccctgatg agattcgtcc
     4201 agccagtagc cgatttcagc agttttattc agtggttcaa tacgattaaa cgagataacg
     4261 ccgataagtt catcttcttt gaaaatcatg aacattttgg catagccgcg ttgatgcaac
     4321 atcacattac cctgcaccgt ttttcgcgtg tcctcttcac tctgaacgaa ctgcggccag
     4381 tttagcgact gctgtaacca ggttttattt ttacagatta actgataaag aggtgtgacg
     4441 tgactttccg caacagcatg taattcaagt gattcgctta cttttatcgt ttcagtcatc
     4501 ttatctccag tcaatccatg ctgttgttaa aaaagccgcc tggctggcgg cttaggaaag
     4561 tttacacctc tttgacgctc tcctgctgca ggattttgag catctcatct tttaactgta
     4621 gcttttgttt tttcatgcgg accacttccg cattgtaccc tcgaccgtcg gaaccttcct
     4681 ttctggcaat ttcatgatca agtttattgt gtttatcgaa caaggacata aagcgaggat
     4741 tttcgttttt cagacgggat attaaatctc ggtattctgg aaacatacgc actccctgtt
     4801 agtggctgac acggtgtatc tgaaatacac accatcagat tactcattta acttagggaa
     4861 aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc gaagaaggtg ccgaagaaaa
     4921 aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt tatccggcgc tggcgggaaa
     4981 tactgataca gccatgtttc actgatagcg tccccctgac aacgtagata catccgcata
     5041 tcgaccggat cagtggagtc cgaagtcgga taccagtcaa actgaatacg ataaccatcg
     5101 atgggttcaa tatagagaat ttcgatttgc ttcgcttccc cactggaaag cgtaatcacc
     5161 ggctcaatgc cttttggcgc ggcagctttc agatcaccac caacgaaatc gacggcaaaa
     5221 cgacgcgccc atttttcggg atagtgttca cctggcgccc aaccttccgg gaaaccgccc
     5281 atgccggtac gcgtcgccat aacgcgcgct aatgggcaat gaacaggcgg ttgcgcactc
     5341 cagtacagac gatactggaa tgcaaactca tcacctgctt ttacagcttt ttctggctgc
     5401 cagaagcaga caatgttatc cagcgtttcg cccgttgttg ggatttccat caggccgatg
     5461 gtgcccttac cccacttgtt acgcggttcc acccacagac ttgggcgttt gttataccag
     5521 cccataatgt cctgataatg ggagaagtca cgatccagtt gcaataaacc aaaccctttc
     5581 gggttgttgt cggtgtaagc attgaactgc aatttttgcg gattattcag cggacggcaa
     5641 atccactcgc cgttgccccg ccacatggac agacgatcag agtcatgaat ttgcggatga
     5701 attgtatcgc acatccgacg ttcattagta ccgcagctga acatactggt catcggcgaa
     5761 atgcccagct gtttaatgtc tttgcgcgca tacaggtgat tttccacatc cataatcacc
     5821 tgacttttct cacaatggat agtgaactta taggcaccag taatgctggc gctatcgagc
     5881 aacgcataaa cggtaaaggt agttgccccc ggttttaccg tatcaaacca gaaggcggta
     5941 aagtcgggga actcttcttt actgtcggtg taagtgtcga tcgccaggcc gcgggccgac
     6001 aaaccgtatt gatatgtatc atcaacggcg cggaaataac tcgcgccgag aaatgatact
     6061 acatcacggc gcgccagttc gggggcttta aacacgcgaa aaccggcaaa gccgagatcg
     6121 ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt tgtatttgaa caactccggg
     6181 cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta cagaaaacat acgaacgcgg
     6241 cgacggaatc ccattcccat atggaagaac tgagcgtcca gttgacggtt ctcaacgtta
     6301 tgccagagcg atttttcggc gtcgtattga atactgttat aagcctgcgg cgtcattgtc
     6361 gccagcgtgt caggtaacgg acgaggcgca ccacgccacg ctgtttgcgc taagtcgtgc
     6421 gccattgact gtagaatgga gaagtcaaaa cgctgggttt gcccgtcggc aatatcagaa
     6481 tctgccgcga atgctgcctg agaaaaaaga gaagcaatgc cgctggtacc gcacacggcg
     6541 gccatagcca ttaaaccttt aataaatcgt ctacgatcca tacctgaaag tgagtccttc
     6601 tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc aagaaagtgc ttatttatga
     6661 ccgctcactc tagacaatat ttattaataa tccaattgtt gaactgagag aatatgcata
     6721 aatcggcatt ttttttatgt cgatgggatg agcctgattc gtttgtaaaa tcaggcaaat
     6781 ttttgcaaag gcggatggtc agaatttaaa gcaaaccaca gcgacgttta ctgcgttctt
     6841 ccgcttcctg gtaaagctgg aactcatcca aaaccggaca acccaacgcc tgctcaaatg
     6901 cttcgcgact ggcattaccg tgactgcgtg tctgcgtttc attacccaga tttgactgga
     6961 agatccccgc cgcgctaacg ggcaagaaat cttcataggt gatgggttgc gccactaccc
     7021 aaccacgttc aattaagggc tgtggatcgt ctccaggatg aatcgcctga cgatgcgcct
     7081 cacccgaagg cgtcagacgg taccggaacc atgccaaccc ttgctgacgc attaaaaact
     7141 cactgtcagg aaaagtgcgg aaggtttcct gtaaatgcat ttggtgagtg agattatcct
     7201 gcccggttcc agcgttacgc agaagatcat catacagttg tcgccctttc ggcgttaatg
     7261 ccacgccacg ctgctcaatt tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg
     7321 caaacaacac cgtctcttcc agtgctttaa agctggtctg gcgtagtaaa atcggtacct
     7381 cgcggcgcgg cggcccctcg atgagaattt tgggttcaat tccgcattca ggcgtcatcg
     7441 actgcacccg gtcaatatcc agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa
     7501 aacagaccac atcagcaatc aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat
     7561 ctaccgttgc taactggtgc cagcgaaacg tttccagggc ttcctgcaca aactcctgtg
     7621 cctgtgtttc gttaaaaccg ccctgctgct catattcctc taacagttgt cgacaacgtg
     7681 gggtgaagat atcgcgctga cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct
     7741 cgataagctc aaggcggagt aaggatgtaa aaacgcggaa gggattacgc gccagagaag
     7801 catcatcaat gggccgaaat gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt
     7861 aatagctcac cgggtacatc cccataatgg caaacatccg ccgaagagta gcaagctctt
     7921 gtgcagtccc aacgcgaatc gccccatgac gttcaacatt cagtcgcgcc agctcgtctg
     7981 catttaccat tttttcgtgc agttgaggat tgttttccag cacagccaga ttcacatcag
     8041 ctaccagttc cagcagcgtg ccatattgcg gaacttcttg ctggtacatg gctgacattg
     8101 cctgcgaaaa ctgttcccga atctcatccg ccgtgatgct gttcgccata gtgtctgcct
     8161 ccagtggtca gtaatctgga gtgtaggtaa ccgcattcac tctggcggga agaatttaca
     8221 aactgtgatc tcgccgcgaa aacatcaata ttatccattt tgctgtaaca taattgcttt
     8281 aattattaat aatattttgc aatcaagtta tcataatcaa acaacttcac ttgtcagcga
     8341 caccgcttcg tttttaacat cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg
     8401 tttgcgccac cttcatacat tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcagc
     8461 tgaaaccctt aatttgagtc aacctgcgct ctctaataca ttgaatgaac tggagcagct
     8521 gacgggcgct cgcttgtttg agcgtggtcg tcagggggcg caacttacct tacccggcga
     8581 acaattttta acgcatgcag tcagagttct tgacgccatc aacactgccg gacagtcgct
     8641 tcatcgtaaa gaaggtctta ataatgatgt cgtcagggtt ggtgcactac ctactgcggc
     8701 actggggata ttaccttcgg ttataggtca gtttcatcag caacaaaaag agacgacctt
     8761 gcaagttgcg acaatgagta accctatgat tctggcgggt ttaaaaaccg gggaaatcga
     8821 tatcggcatt ggtcggatgt cagatcctga actgatgacc gggcttaatt acgaactgct
     8881 gtttcttgaa tcgttgaagc tggttgtccg ccctaatcac ccgctacttc aggagaacgt
     8941 aacgctaagc cgggtgctgg aatggccggt cgttgtatca ccagaaggca ctgcgccacg
     9001 ccagcattca gatgcattag tgcagagcca gggctgtaaa attccttcgg gttgtatcga
     9061 aacgctgtct acttcgctat ctcgtcaact taccgttgaa tacgattacg tgtggtttgt
     9121 cccttctggc gctgtaaaag acgacctgcg tcatgccacg ctggtggccc tgcctgttcc
     9181 gggacatggt gcaggcgaac cgattggaat actgacccgc gtagatgcga cgttctcttc
     9241 tggttgccag ttgatgatta acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg
     9301 atctgtcgat ccctccttga acattttcac accgtagcga aactaactgg ttcacccgct
     9361 ccgcgaggtt ctgccgacac agaatgtttg ttcagacgga atacgttcac cgcctcagtt
     9421 aatcgtgcag cctgttcttc aagggacacc gccgctgctg aggcctcttc taccagagaa
     9481 gcattctgtt gcgtcacctt atccatttca gaaatcgcct ggctaacctg agttatgcct
     9541 ctactttgtt catccgaggc ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg
     9601 gcatcaacaa tagtgctcat ggttttccct gccgttgcca cctcatccga ccccaggtca
     9661 attaacctga ctgattcact gatcaagcct tcaatctctt tcgctgcctg agcgctgcgg
     9721 cttgcgagtg tccgtacttc gctggcgaca acggcaaatc cacgcccttg ctcacccgct
     9781 cgcgcggctt caacggcagc attcagtgcc agaatattcg tctggaaagc aatactgttg
     9841 atgacggcgg tgatctcaga aattttcttc gaactcgtgg agatagcgcc catcgttttt
     9901 actacaccgg aaaccgtctg cccgccatcg ctggctttaa tagaagcctc ttgcgccagt
     9961 ttgctggcat gatgcgcgtt atccgcattc tgtttcaccg tcgcagtgag ttgctccatg
    10021 ctggcggcag tttgttcgat agccgccgct tgttcttcgg tgcgagatga caggtccgca
    10081 ttgccagctg aaatttcgct ggtgccacga taaatctctt ccgcgccctg tcgaacagtc
    10141 cctactgtca tccccagtga atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt
    10201 tcattacgac ccgccggttc atcattcatc gtcagatcgc cactggcgat tttttcaatc
    10261 cgttgtgcgg catgttgcag tggacgaatg acgatccgac gtagcaccat aaatgttatc
    10321 agcgtcatga ccagggcaag cacaaacgcg ccaatcatga acatcccacc caggcgggtg
    10381 cgctgatggg ccagttccgc cagttggttg gctctggtgc tacgtatctt aacggctttg
    10441 ttcaaaatat cggtataagc attatccagc ggtcggatct gctcactttc atgattgata
    10501 atcgcttcaa acatgccatt tttggcatat ttcaacatag gttgcatacc cgtgatataa
    10561 gcctgaaagc gttgatttaa ttcagtgtcg aaggcttcat cagcaggtgt tttcaccggt
    10621 cgattctgat aagcacgata accttgctgc gactgtttaa tctccgattc ggcttgcgca
    10681 atatttcgct tcattgcttc catttccgca atacgactcg cagccccggc ctgaatcata
    10741 ttgatccggg ctgaacgcaa aaaatctgaa ctgttcgcta acccggtccg aatctcaatc
    10801 tccgcagtaa catcacgctg atcgcgatcg gcctgccaga ggaaatagcc agccagagca
    10861 gaacttaatg cgaatagaac taagataccg ccaagaatgc aggcaaataa cggaaccagc
    10921 ctgatgtgat gcaaaaaacc taatcgctgt gagggagttg tattcattac cgttgtctct
    10981 cgtccaggtt tactcctgat agaaagtcat cggcacttga cggtaattac ttatgaccaa
    11041 aaacctcttt gcgtcacatt tttacaacaa ttattgcaga gaaaattaat gctattggaa
    11101 tgaagagagt aacgaatggc taaactggct aacccgaatg acgaaaaatt ctggaaaaca
    11161 gcacgcaatt tcgtgaagtg attattttcg tccctaagga aacctcatag gatgcctgcg
    11221 ggtgcctgag actttctgtt ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc
    11281 gatatttcta tcaactgtac tgcacccatt ttgttggacg atgaaatgga atagccccta
    11341 atatgtcaaa gccaaaatac ccttttgaaa agcgccttga agtcgtgaat cactacttca
    11401 ctactgatga tggttacagg atcatctcgg cacgttttgg tgtcccccga acccaggtca
    11461 ggacatgggt tgccctctat gaaaaacatg gagaaaaagg tttaattccc aaacctaaag
    11521 gcgttagtgc tgatccagag ttgcgtatta aggtcgtgaa agctgtgatc gagcagcaca
    11581 tgtcccttaa tcaggctgct gctcacttta tgcttgctgg tagtggttct gtagccaggt
    11641 ggctgaaggt ctatgaagag cgcggagaag ctggtttacg cgcgctcaag attggcacca
    11701 aaagaaacat tgcaatatca gttgatccag aaaaagcggc atcagcattg gagctgtcaa
    11761 aagaccgacg cattgaggat cttgaaaggc aagttcgatt tcttgaaacg cggcttatgt
    11821 atctaaaaaa gctgaaagcc ttagctcatc ccacgaaaaa gtgaaagtac tcaacgagct
    11881 aaggcagttt tatcctcttg atgagcttct cagggctgcg gagataccgc gcagtacgtt
    11941 ttattaccat ctaaaggctc tcagcaagcc tgacaagtat gcggacgtta aaaagcgtat
    12001 tggtgagatt tatcacgaga atagaggccg atacggatac cgtagggtaa cgctgtctct
    12061 tcatcgagaa gggaaacaga ttaaccataa agctgttcag cgcctgatgg gaaccctctc
    12121 acttaaagca gcgattaagg tcaagcgata ccgctcttac agaggagagg tagggcaaac
    12181 cgcccctaat gttctccaaa gagatttcaa ggctacgcgg ccaaacgaga agtgggttac
    12241 cgatgttact gaatttgcag tcaatgggcg caagctgtat ttgtctccag taatagatct
    12301 cttcaacaac gaagttattt cttacagcct ttcggaaaga ccagtgatga acatggttga
    12361 gaatatgctc gatcaggcat tcaaaaagct taatcctcac gagcatcctg ttctgcactc
    12421 tgaccaggga tggcagtatc gtatgagaag atatcaaaat atccttaaag aacatggtat
    12481 taaacaaagc atgtccagaa aaggcaattg tctggataat gctgtggtgg agtgtttctt
    12541 tggaacctta aagtcggagt gtttttatct tgatgagttc agtaatataa gcgaactgaa
    12601 ggatgctgtt acggaatata ttgaatacta taacagcaga agaattagcc tgaaattaaa
    12661 aggtctgact ccaattgaat atcggaatca gacctatatg cctcgtgttt aactgtccaa
    12721 ctttttgggg tcagtacaaa gtgaggctac cctatgcctg aacaccataa ggttagcctc
    12781 ttactcgttg gaaatcaaca cagggggctg ggaatgccgc agaaatatag attactttct
    12841 ttaatagtga tttgtttcac gcttttattt ttcacctgga tgataagaga ttcactgtgt
    12901 gaattgcata ttaaacagga gagttatgag ctggcggcgt ttttagccta caaattgaaa
    12961 gagtaagagt cttcggcggg aaattattcc cgccttactt acggcgttgc gcattctcat
    13021 tgcacccata tttattcttc acaaaaataa taatagattt tattacgcga tcgattattt
    13081 atttcctgaa aaaaaataaa aaaatccccg ccaaatggca gggatcttag attctgtgct
    13141 tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc
    13201 gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc
    13261 agagaagtgt acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt
    13321 gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta
    13381 ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga
    13441 atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct
    13501 ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc actcattggc gggtgatgca
    13561 acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt
    13621 aaacagcaat aaagtggata aaagtctatt tcatcaactg cttacttaat gccgggttgg
    13681 cttgaatcaa acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc
    13741 attcctttac catggcgact gatacgccca aaacccgggc gaaatcatcg atttttaatc
    13801 ctgtcccttt tcataactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa
    13861 ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg
    13921 gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga
    13981 agctcattaa gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg
    14041 gctatgattc gattcagcct gtcatttagc gtgacttata gccacctaac gatctgataa
    14101 atgccgatat atctcttatc actcctgttt tgtaaagatt tcgcaaatct aaccttctgc
    14161 gaaggcgtaa atctctggct acaaaaaagg caacggcact atcatttttt aacaatgaaa
    14221 caacaaatta accttgtttc attttctcca tgattttacc aggaagatta cgtagaaagg
    14281 gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg
    14341 cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt
    14401 ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt gataaagaag
    14461 gcgatcagcg taaagcgttt atcgacttcc tgcaaaatac agtgatgcgt agcggtgaac
    14521 gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc tctgattacg
    14581 cgattctgta tggttattct cagcaggtga atcaggcgat ggattccggt ctgcgcccgg
    14641 tcgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa agcggtccgc
    14701 tgcgtgaaat gaatggttcg ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc
    14761 aggccgatgc tgcgcactct gcgctaaaac agagcgatga cctgaaaccg gtatttgatc
    14821 aggcattcac caaagtggta acgacgccag ctgatgcatt gcaaccgttg attccggcgg
    14881 cacaaacttt cacgcaacaa ctggtgatgg ttggagacta cattgctcag caaggtactc
    14941 aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc acagcaggca agtgaatata
    15001 acaaactgat tgcgccatta ccggcacaac atcaggcgtt taatcaggcc tggactacag
    15061 cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt tacttcacgc
    15121 agtcgcagct tagtccgcga cgtgaggatt cacacagttc ttctcaacct ttccttgtaa
    15181 cgcatcaatc aaattatcca cggcacaggc ggccatgcca taacgcgtct catgggtggc
    15241 agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg aatctacgga
    15301 cagtggctct tgttcgaaga catccagccc ggcggcgtga atttcgcctt tctgcaatgc
    15361 tgcgatcagt gcattttcgt caaccaccgg gccacgtccg gcattaatga aaatggcgga
    15421 ggatttcatt ttggcgaatt gttctgcgcc aaacagatga tgcgtctcat cagttaacgg
    15481 caggatcagg caaacgaaat ctgactcttg taacagagta tccaaatcgc agtagcgggc
    15541 gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt
    15601 gaagccaaag tgcacacgtt gtgccagcgc catgccgatc cgtcccatcc cgacaatgcc
    15661 cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg cggtccattc
    15721 gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca acaccagcgc
    15781 catcagcgta tcggcgacgg tttctgttaa tacggttggc gtgtgcatca gcagaatttt
    15841 tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg ttgatgtggc
    15901 acgcagtttc ggcatttttt ccagcaatgc ggcatctaca ttctcgtttg aacccagtaa
    15961 accttcagct tctgcaaaaa ttgctgcatt ttgaacgacg gtttgtgggc tgaggtttgc
    16021 cacctggtga acggtgaaat gcgcttgcag gcgttgcagt aaatcatcag gtaaggcttt
    16081 gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg atcaccttag
    16141 cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc acctggcatg
    16201 aaagggatta cagcgggctt aaggtaattt ctacacggcg gttttgcgcc ttaccttctg
    16261 cggtgctgtt gctggcgatt gggttagccg ggccaaggcc ctgagtacgg atgcggctgg
    16321 cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc tgggagagac
    16381 gcatgttcag gtcgtgaccc cccgtgctgt cggtataacc aatcacgtta accgccgttt
    16441 tcggatactc tttcagcacc attgccacgc cggtcagggt gttagcgcct gccggtttca
    16501 ggggagcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata atgttatccc
    16561 cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc acgcagcttc gcttcctgca
    16621 catccatgta ataacccacg ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg
    16681 cgcctttacc gcgatctttc ttcgaagaag agagcgcacc aatacccgcg cccacgagag
    16741 agcccagacc tgcgccgata gcagatttgc ctgcttcgcg ttcgccggtg taagggttag
    16801 ttgtgcagcc agataccgcc agagcgccac tcactacggc ggcaagtaag ataaacacgt
    16861 ttcttcattg ttaatcctta ataacctttt tattctttgt cacgggttcc gtggcgggag
    16921 attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat
    16981 ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg
    17041 atattccgtt ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac
    17101 cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt
    17161 tcgcgaccag gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct
    17221 cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg
    17281 ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc
    17341 ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc
    17401 gacattatta caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta
    17461 ttcgaccggt gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca
    17521 acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc
    17581 taacccactc aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta
    17641 cttttccgca ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga
    17701 aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt
    17761 tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt
    17821 tctggcgcgt tgcaccacag gttatgtcgt cttcgcctct tatttgctgg gcgagagtga
    17881 cggaatagcg aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat
    17941 ccgcgagctg gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat
    18001 gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt
    18061 ggggcaaatc ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg
    18121 taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg
    18181 cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg
    18241 cgcatatcaa cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc
    18301 gggcggtgcc aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa
    18361 accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat
    18421 tgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag
    18481 taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga
    18541 ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac
    18601 ggaaggaaaa agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg
    18661 ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat
    18721 tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga
    18781 tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat
    18841 tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg
    18901 gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct
    18961 gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc
    19021 tgtcaccatt catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg
    19081 ggtgtggaac tcccgtgggc agatccttgc cggagcggtc attagcgagg gaattaaacc
    19141 tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat
    19201 ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa
    19261 tggctgtgcg ggtaatacga cgctggcatg gctggaaaaa tacaacggtc cggaactgac
    19321 acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc
    19381 tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga
    19441 tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc
    19501 gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg
    19561 ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc
    19621 gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt
    19681 ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct
    19741 gaacgttgcg cttcccgaat catggtttat ttcccagata gcaacagcag ccaaccacat
    19801 gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact
    19861 taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa
    19921 tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa
    19981 agtcgggaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa
    20041 ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat
    20101 cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt
    20161 tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaagcaga
    20221 tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg
    20281 caatataaag cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt
    20341 acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta
    20401 atacagggct actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc
    20461 tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc
    20521 tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag
    20581 aatatatcgt tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc
    20641 atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg
    20701 gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg
    20761 gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg
    20821 atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc
    20881 cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc
    20941 tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc
    21001 aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg
    21061 taggcgctga tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag
    21121 aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac
    21181 gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact
    21241 taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt
    21301 cattccgttc tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat
    21361 ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa
    21421 attatcagcg tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg
    21481 ggtcggttta tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg
    21541 taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg
    21601 ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt
    21661 tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa
    21721 gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact
    21781 gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg
    21841 gtctgggtag cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg
    21901 aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt
    21961 taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt
    22021 acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga
    22081 ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg
    22141 cacaccttga tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt
    22201 caggtcgtct ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca
    22261 ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag
    22321 tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct
    22381 ttccatcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga
    22441 tttacctcgg tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg
    22501 gtttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta
    22561 cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct
    22621 cagccgcgag ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata
    22681 aacaatgact cgtaaaattt gggccagtta acaggccctt tttactgctt tttgtaacgc
    22741 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
    22801 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
    22861 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
    22921 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
    22981 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
    23041 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
    23101 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
    23161 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
    23221 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
    23281 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
    23341 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
    23401 agggcagcgt attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc
    23461 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
    23521 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
    23581 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
    23641 gaagaagttt acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat
    23701 cggtgaaacc acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc
    23761 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
    23821 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
    23881 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
    23941 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta
    24001 tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct
    24061 ggtagacgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct
    24121 gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg
    24181 gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta
    24241 tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga
    24301 taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca
    24361 cctgcacggc acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt
    24421 ctggatgtca gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa
    24481 aaaagaagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg
    24541 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
    24601 cccgcagaca aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa
    24661 aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca
    24721 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
    24781 aatcctctat caccgaccaa attcgaaaag cctgctcaac gagcaggttt ttttgcattt
    24841 gtagctcatg aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat
    24901 aagacgcgcc agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac
    24961 cgccttattc gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact
    25021 atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt
    25081 ttgcgttact tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt
    25141 aatcgctcag atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc
    25201 accaaatgtt gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc
    25261 tctgctgcaa atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg
    25321 tgtaaatccc ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg
    25381 aggggcattt tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca
    25441 cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact
    25501 gcaaagggtt aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa
    25561 aagtcaggga tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt
    25621 caggctaaaa ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg
    25681 tttacctccg gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa
    25741 tttaaaatcg gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa
    25801 gacggtaaaa cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa
    25861 ttcaaaccga cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa
    25921 aacgcgcaaa acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg
    25981 ggcttgccgg agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg
    26041 ctgactcgac cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg
    26101 tcaaaagaat atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac
    26161 ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac
    26221 aaagcgtttg atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt
    26281 acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg
    26341 tacccgaacc cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa
    26401 atgccggggc tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac
    26461 gtgaaagttc gccaagcact aacctacgcg gtgaacaaag acgcgatcat caaagcggtt
    26521 tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac
    26581 gacgacgttc aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg
    26641 ggtctggaaa aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac
    26701 ccgaacgctc gccgcatggc ggagatgatt caggcagact gggcgaaagt gggcgtgcag
    26761 gccaaaatcg tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat
    26821 cagacggtaa tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc
    26881 ctgttcagct gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg
    26941 tttgaagatc tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg
    27001 tacaagcaag cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc
    27061 accgtgtttg aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa
    27121 catcacttcg aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg
    27181 caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt
    27241 tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac
    27301 tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag
    27361 caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc
    27421 agttccaggc tgcgagtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg
    27481 tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca
    27541 catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg
    27601 tcacgtgcag ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca
    27661 ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt
    27721 atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat
    27781 ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat
    27841 tttcgatcac actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg
    27901 gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg
    27961 cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga
    28021 caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc
    28081 cgccattgtg ctgggtacta ttccgctagc ggtcattgtg cgtatgacac gctcctcgat
    28141 gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat
    28201 gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg
    28261 cctccaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc
    28321 cggtctgggg cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg
    28381 cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta
    28441 cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac
    28501 tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta
    28561 ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat
    28621 cgcgatcttt gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct
    28681 gctcgccccg ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga
    28741 cgtcggtcgt gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg
    28801 ctgtctggtg gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta
    28861 cttcggcggc ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc
    28921 aagcctgctg ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc
    28981 gctggcgctg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct
    29041 ggtggaggtg aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg
    29101 ccagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct
    29161 cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca
    29221 gccgccaacg cctgagtggg gcactatgct ctccgacgtg ttgcagttcg cgcaaagcgc
    29281 ctggtgggtc gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct
    29341 gatgggtgac ggtctgcgtg acgcgctcga tcccaaacta aagcagtaag aggttcgaga
    29401 tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc
    29461 gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg
    29521 agtccggctc cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg
    29581 gccgcgtaat ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa
    29641 aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca
    29701 gccttaaccc gtgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg
    29761 gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc
    29821 ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc
    29881 gcgtgatgat cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga
    29941 ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc
    30001 agaaagagaa catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg
    30061 cgcataaaat cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca
    30121 tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg
    30181 ctcaggacaa agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc
    30241 cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag
    30301 aaccggcgct gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg
    30361 atgccgggag gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca
    30421 ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct
    30481 ggtgaaggcg ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt
    30541 gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc
    30601 caccggcggc gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca
    30661 gaagctgcgt cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc
    30721 gcgtaagaaa gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa
    30781 agaacagcgt cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca
    30841 ctatgaccgc tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg
    30901 tggtctgatg ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt
    30961 ctcagtgcgt gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc
    31021 ttatgtcttt atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt
    31081 gatgtaccta ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg
    31141 ccatccgtac acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg
    31201 tgaacgcatc aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc
    31261 cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa
    31321 agactacgga ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg
    31381 ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga
    31441 caagcatagc aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc
    31501 cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa
    31561 cgggcttccc aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa
    31621 cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt
    31681 atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag
    31741 ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg
    31801 aatgatatgt ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa
    31861 ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat
    31921 caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag
    31981 tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa
    32041 taccgcagaa aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc
    32101 ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac
    32161 gacaataatc cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc
    32221 accaccagac gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg
    32281 tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac
    32341 ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa
    32401 aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc
    32461 gctttcagaa agggttttta aataaatgtc ctgcactacc caggtgacag gataagcaat
    32521 gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac
    32581 ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt
    32641 aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga
    32701 aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag
    32761 tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc
    32821 gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc
    32881 aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg
    32941 tgctgacagt aaaaagca
//
LOCUS       DAJTNW010000040        32632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTNW010000040 DAJTNW010000000
VERSION     DAJTNW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            700..1128
                     /gene="uspC"
                     /locus_tag="OO179_001963"
     CDS             700..1128
                     /gene="uspC"
                     /locus_tag="OO179_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5532041.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1135..2549)
                     /gene="otsA"
                     /locus_tag="OO179_001964"
                     /pseudo
     CDS             complement(1135..2549)
                     /gene="otsA"
                     /locus_tag="OO179_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2524..3324)
                     /gene="otsB"
                     /locus_tag="OO179_001965"
     CDS             complement(2524..3324)
                     /gene="otsB"
                     /locus_tag="OO179_001965"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5532042.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3491..4477)
                     /gene="araH"
                     /locus_tag="OO179_001966"
     CDS             complement(3491..4477)
                     /gene="araH"
                     /locus_tag="OO179_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5532043.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4492..6006)
                     /gene="araG"
                     /locus_tag="OO179_001967"
     CDS             complement(4492..6006)
                     /gene="araG"
                     /locus_tag="OO179_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5532044.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6076..7065)
                     /gene="araF"
                     /locus_tag="OO179_001968"
                     /pseudo
     CDS             complement(6076..7065)
                     /gene="araF"
                     /locus_tag="OO179_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7862..8365
                     /locus_tag="OO179_001969"
     CDS             7862..8365
                     /locus_tag="OO179_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5532045.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8445..8696)
                     /locus_tag="OO179_001970"
     CDS             complement(8445..8696)
                     /locus_tag="OO179_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5532046.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8811..8897)
                     /gene="azuC"
                     /locus_tag="OO179_001971"
     CDS             complement(8811..8897)
                     /gene="azuC"
                     /locus_tag="OO179_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5532047.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9160..9483
                     /locus_tag="OO179_001972"
     CDS             9160..9483
                     /locus_tag="OO179_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5532048.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9654..10151
                     /gene="ftnA"
                     /locus_tag="OO179_001973"
     CDS             9654..10151
                     /gene="ftnA"
                     /locus_tag="OO179_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5532049.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10188..10427)
                     /locus_tag="OO179_001974"
     CDS             complement(10188..10427)
                     /locus_tag="OO179_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5532050.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10617..11828
                     /gene="tyrP"
                     /locus_tag="OO179_001975"
     CDS             10617..11828
                     /gene="tyrP"
                     /locus_tag="OO179_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5532051.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11890..12555)
                     /gene="yecA"
                     /locus_tag="OO179_001976"
     CDS             complement(11890..12555)
                     /gene="yecA"
                     /locus_tag="OO179_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5532052.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12751..12837)
                     /locus_tag="OO179_001977"
     tRNA            complement(12751..12837)
                     /locus_tag="OO179_001977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12801..12803),aa:Leu,seq:taa)
     gene            complement(12850..12923)
                     /locus_tag="OO179_001978"
     tRNA            complement(12850..12923)
                     /locus_tag="OO179_001978"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12889..12891),aa:Cys,seq:gca)
     gene            complement(12978..13053)
                     /locus_tag="OO179_001979"
     tRNA            complement(12978..13053)
                     /locus_tag="OO179_001979"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13018..13020),aa:Gly,seq:gcc)
     gene            complement(13205..13753)
                     /gene="pgsA"
                     /locus_tag="OO179_001980"
     CDS             complement(13205..13753)
                     /gene="pgsA"
                     /locus_tag="OO179_001980"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5532053.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13810..15642)
                     /gene="uvrC"
                     /locus_tag="OO179_001981"
     CDS             complement(13810..15642)
                     /gene="uvrC"
                     /locus_tag="OO179_001981"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5532054.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15639..16295)
                     /gene="uvrY"
                     /locus_tag="OO179_001982"
     CDS             complement(15639..16295)
                     /gene="uvrY"
                     /locus_tag="OO179_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5532055.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16591..16767
                     /locus_tag="OO179_001983"
     CDS             16591..16767
                     /locus_tag="OO179_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532056.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16754..16978
                     /locus_tag="OO179_001984"
     CDS             16754..16978
                     /locus_tag="OO179_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5532057.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17011..17631)
                     /gene="sdiA"
                     /locus_tag="OO179_001985"
                     /pseudo
     CDS             complement(17011..17631)
                     /gene="sdiA"
                     /locus_tag="OO179_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17861..18613)
                     /gene="yecC"
                     /locus_tag="OO179_001986"
     CDS             complement(17861..18613)
                     /gene="yecC"
                     /locus_tag="OO179_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5532058.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18610..19278)
                     /gene="tcyL"
                     /locus_tag="OO179_001987"
     CDS             complement(18610..19278)
                     /gene="tcyL"
                     /locus_tag="OO179_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5532059.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19293..20279)
                     /gene="dcyD"
                     /locus_tag="OO179_001988"
     CDS             complement(19293..20279)
                     /gene="dcyD"
                     /locus_tag="OO179_001988"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5532060.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20384..21184)
                     /gene="tcyJ"
                     /locus_tag="OO179_001989"
     CDS             complement(20384..21184)
                     /gene="tcyJ"
                     /locus_tag="OO179_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5532061.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21272..21823)
                     /gene="fliZ"
                     /locus_tag="OO179_001990"
     CDS             complement(21272..21823)
                     /gene="fliZ"
                     /locus_tag="OO179_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5532062.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21869..22588)
                     /gene="fliA"
                     /locus_tag="OO179_001991"
     CDS             complement(21869..22588)
                     /gene="fliA"
                     /locus_tag="OO179_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5532063.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22753..24405)
                     /gene="fliC"
                     /locus_tag="OO179_001992"
     CDS             complement(22753..24405)
                     /gene="fliC"
                     /locus_tag="OO179_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5532064.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24671..26077
                     /gene="fliD"
                     /locus_tag="OO179_001993"
     CDS             24671..26077
                     /gene="fliD"
                     /locus_tag="OO179_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5532065.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26102..26512
                     /gene="fliS"
                     /locus_tag="OO179_001994"
     CDS             26102..26512
                     /gene="fliS"
                     /locus_tag="OO179_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5532066.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26512..26877
                     /gene="fliT"
                     /locus_tag="OO179_001995"
     CDS             26512..26877
                     /gene="fliT"
                     /locus_tag="OO179_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5532067.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26955..28442
                     /gene="amyA"
                     /locus_tag="OO179_001996"
     CDS             26955..28442
                     /gene="amyA"
                     /locus_tag="OO179_001996"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5532068.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28476..28889)
                     /locus_tag="OO179_001997"
     CDS             complement(28476..28889)
                     /locus_tag="OO179_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5532069.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29076..30281
                     /gene="yedE"
                     /locus_tag="OO179_001998"
     CDS             29076..30281
                     /gene="yedE"
                     /locus_tag="OO179_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5532070.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30278..30511
                     /gene="yedF"
                     /locus_tag="OO179_001999"
     CDS             30278..30511
                     /gene="yedF"
                     /locus_tag="OO179_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5532071.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30618..31288
                     /locus_tag="OO179_002000"
                     /pseudo
     CDS             30618..31288
                     /locus_tag="OO179_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31328..32536)
                     /locus_tag="OO179_002001"
                     /pseudo
     CDS             complement(31328..32536)
                     /locus_tag="OO179_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8173 a         8334 c         7718 g         8407 t
ORIGIN      
        1 aattagcatt agaatagttg cgataagctg caataagcag agccaccttt ttggtttaat
       61 aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc
      121 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg
      181 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
      241 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
      301 aggaataatc gaaatgggat gttgcgcacg gttaaaataa ctcaccgtaa ataatcatct
      361 gctataaata atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg
      421 cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta ataaaatgtt
      481 tttcatatat taaccacttg tttgatgcgg ggaatcagta cattattttc ttataattac
      541 agttgaaatc atatgatcac cgagagtgat tgattaattg tctttactaa taatcagact
      601 aatatttacc tgtttgactg agttgggatt gcgtcgtttc tccattagga gtaaagcttt
      661 aatgtcacct gaagttcaca gcagaataag gaacaggtta tgagctatag caatattctt
      721 gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt atctatcgcc
      781 aggccagtaa agggacacat cagtttaatt actctcgctt ccaacccgga aatgtacaat
      841 caattagccg cgccgatgct tgaagattta cgcagcgtga tgcaagaaga aacgcaaagc
      901 tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt tattgcctac
      961 ggtgagttaa gcgaacatat tctggaagta tgtcacaaac atcatttcaa tttggtgatt
     1021 tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa aagagtgatt
     1081 gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gagattaacc gctcctacgc
     1141 aagctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc gcggaactat
     1201 ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt tcacgataac
     1261 gtccagcatt tctgcatgac gggaaatacg ttccaccagc gacatagtca atgcacgatc
     1321 cagcgcagct gcaacttcgt cacgatcgta ggggttaaca attaacgccg acgttaactc
     1381 gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc gggttggctg ggtcctgagc
     1441 agcaacatac tcttttgcta ccaggttcat cccgtcacgc agtggcgtca ctaagcccac
     1501 gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat tcaaataata
     1561 aagcggcgtc cagcctaatt gcccgtattt accattaatt cgtccagctt cattttcgag
     1621 ctgatgacga atatcctggt aggcttgcac atcaccacgc gacgttggtg caatctgggt
     1681 ataacgaatt ttaccatgat gctgcggata tttttccagc aatgcctcat aggcgagaaa
     1741 acgctccggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
     1801 tttcaattcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
     1861 tatttctttc ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt ttccccaggc
     1921 tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg ttagaaaggc aatccaggaa
     1981 cgccagacga tcgttttctg tctgtaaacc cagcaaatca taatcacaaa gctgttcaag
     2041 caaggtgtca tatgtcggca gcgcgttgaa aatttccggt gtcgggaaag gaatatgcaa
     2101 gaaaaaacca atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag
     2161 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
     2221 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
     2281 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
     2341 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga
     2401 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc tttcagtgcc
     2461 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
     2521 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
     2581 ttgtaatgcg gcggttatca tttcaagcca gctccagaca tccggcacac ccgccagtcg
     2641 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     2701 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     2761 aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac ctctcggttt
     2821 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     2881 ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     2941 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg tatgcagttg
     3001 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt taccattgat
     3061 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     3121 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     3181 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     3241 gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg atagttcagg
     3301 ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta ttgtgttgcg
     3361 cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc catttgcaac
     3421 gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta aattgttgtg
     3481 gaaaaaagca tcagacagtg cgcttcgctt tttgcttgta acggtcgaag atcaccgctg
     3541 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     3601 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     3661 tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata agctcataac
     3721 caatcgacgt cattggctgc ccactagtca tacgtgaagc cagaataatt ccggctatcg
     3781 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtatacccg
     3841 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt attacgacca aaggtggttt
     3901 tattcagcag caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca
     3961 gaccgaacca gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt
     4021 taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgtcgtga
     4081 tcagagcatt tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaacgc
     4141 ccagcaacaa acccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg
     4201 ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag
     4261 caaggcagaa caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc
     4321 ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca
     4381 ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact
     4441 taggtgcgcc agaccccgat gtagaaacag aagacatcat actctcctta ctcaggcaac
     4501 tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg
     4561 taacaattca ccggcgattt cgccttcccg catcaccaca atccggtcgg caacgccgag
     4621 gacttcaggt aagtcgctgg aggcaaacag caccgccacg ccctgcgccg ccagcgcata
     4681 aattacgtta tatatttcgt gcttagcgcc aacatcaatg ccgcgcgtag gttcatccag
     4741 caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc
     4801 gcctgagaga ttcatgatca gttgctccgc gcccggcgtt ttgatgttga gcgaacgaat
     4861 gtgctgatcg gcattgtttt cttcccaact gttgttgatt acacaaccgc cgagcacatg
     4921 tttgcgtctg gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc
     4981 cgctttgcga gcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg
     5041 aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa
     5101 caagcctttc attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc
     5161 accactgcga accgccagac ttattggcgt acgcacgcct agtgctttca cagcatcaag
     5221 acgcaggcgc tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg
     5281 cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt
     5341 gacataacgt ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat
     5401 acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa
     5461 aagattgccg atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt
     5521 agcgttacgc gccagcgctt tggcgatttc aaccatctgc cactgaccaa tggcgagata
     5581 tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc
     5641 ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat
     5701 gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc
     5761 cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga
     5821 acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc
     5881 attttcaccc atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag
     5941 cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg
     6001 ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg
     6061 aatttttccg gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt
     6121 gtcacgcgtg atcagtacca cgtctgtaac ttcggtaaat tttggcgttt caacgtcttt
     6181 tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag
     6241 cagggaaccg tagaagccgg ttgcctgtgc tttagacagt tcgcttactg catccacacc
     6301 gttaatacca ataccgatga tatcggctgc tttaaagccc tgaccttcgg ttgcacgtac
     6361 gccgccaagt acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttccggatg
     6421 ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg
     6481 tacctgataa atttgttttt ccgggaatcc tgcagctttc agtgcgtcca tagaacccgt
     6541 tgtacggcgg cgggcggtat ccagttcgtt ggcggtaatc gccatcactg cgctttcttt
     6601 gacatcccag ccacgtttct gcatctcttt atacagttcc tagccctgac gttcgccaat
     6661 tttagtcgcc gccatcatca ccagaggaac ggtatccatt ggcttacctt tggcgttaac
     6721 aaactggtca tccactgcaa tgactttcat atcgtagcca cgcgctttcg cgacgatggc
     6781 agagccgagt ttcgggtccg gagtacaaat aacgaaacct tttgcgccac tggctgccag
     6841 gctgtcgatc gcgttcaatg ttttttcgcc atccggcacg gcaatcttaa taacctcaaa
     6901 ccctaaatcc ttcccggctt tatcggcaaa cttccattcg gtctggaacc acggctcttc
     6961 cggttgcttc accagaaaac cgagcttcag gttctccgcc atagcggatt gtgacataac
     7021 ggctgccaga ccaatggctg ccagggcttt agtaaatttg tgcatggttc tctccagctt
     7081 tagtgtcgtt ttgtgtagtg caaaaacgaa tgacattcgt taaattaatc ggaaaacaaa
     7141 gcattacctt ttatctaaaa gataagtgac cgtgttgaca tagttttagc gagaaattaa
     7201 ttctccatag gagagcaata tcacatcgca gaattacagt gagaacgtgc ataaatttag
     7261 cgggaaaaga cataaggaaa agccaatttg tcagacaaat tgttgaatgc acagcagatt
     7321 aatccataag attagcctgg aaatccttgt tgtcttcggt acccattcgg gatgtcttct
     7381 ttttaaccag tcaataggcc gcattacctg gcgttgagtt tttgaaatgg tgtaataacc
     7441 acaactcaaa gatgtggaaa atgcgcgtca ttcatttcgc tattaattat cactgtgctc
     7501 attaattaac agaacacgta taatgagagc catctcgcaa aaatgaaaaa acgttttata
     7561 aaatcatcac ttcatcatga attcaaattc atcgattaat atcaacaaga tacaaaaagc
     7621 actatcatta aaattcattg catttacatt gatttcacca atgaaatgta aaaatatata
     7681 aacttgatga tttaagcatt ttcttataac cgttcagacg ttattcttat ttcagatcat
     7741 cgtcagaatt gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca
     7801 gtaattatgg tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg
     7861 tatggcaacc gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc
     7921 atccaatctc taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc
     7981 cactttcctt cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt
     8041 tatgaagagt gtcggcgcta cccccatcgt taaagccatt gatgttccag gtgaaaaact
     8101 gaactctctg gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttcaagcac
     8161 tctggcgcag ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct
     8221 gcgcgatctg gaaaaagaac agcagcatga tggcctgttg ctgcaaacca ttcttgatga
     8281 agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa
     8341 tgtcgtgtca caccagctgc attgatcata catcggcgct aatacattgc gccgatgaag
     8401 tttttgagaa accgctgcct catctgtttg aagcagcggt ttttttaatg ggattcaccc
     8461 tgtggggtaa acttgagttc aatcagcgcg atggcttttt ggattgcccg catggtgacc
     8521 gggtctgcgg cagcgggatg atgagtaaag tcgatattct tcagctgact ggacattttt
     8581 tcacgaactt caacgggcgc gattacatcg agaacatcca gaatttgttt gataaccaac
     8641 tggcaagcta ccacatcaga aaccagttcc tgatcggcat tcagcggctg ggacatcgta
     8701 aactcctgat agcattttga aagccgttat agtagcggct tcacatcttc agcgatagtc
     8761 acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg ttatcggaac
     8821 atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat acttttcagg
     8881 attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt attgtctttg
     8941 cgcttataat atgacaacca tcacaaaaat caatctttat gtgatacaaa tcacataaat
     9001 acccctttaa tgttataaaa atgataatca aaaaacagcc cccctatttc tgacacctac
     9061 agatggcaag aaatagcgcc tgccaggcgt cttttccggc cattgtcgca gcgctgtaac
     9121 gcgtaaaata gtgctttctc ttactcttct ggctggacca tgagacttct gattctgact
     9181 ctttcattaa taacgctggc ggggtgtacg gttactcgcc aggcccatgt gagcgaagtt
     9241 gacgcggcaa caggtattgt acggttggtt tatgaccagg cttttttgca gcatgcccat
     9301 accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat gccagcagga aggctatacc
     9361 cacgcgatcc cctttggtca gccagtaggc aactgtagcc tttttgcggg ttccttatgt
     9421 ttgaatacgg aattcacttt gtcatatcag tgccatcatt ccgccttccc tgtctttctc
     9481 taaatatttt aatcagcgag gggatcttcg ctgattaaag aaatagcggt aatgcgtttt
     9541 attcccattc gtatttttaa taattgaagt ttatatttta ccttttgcaa ataataaaat
     9601 aacaaattat agtgacgcca cagcaacaaa tataaccttt gtggagcact atcatgctga
     9661 aaccagaaat gattgaaaaa cttaatgagc agatgaacct ggaactgtac tcttcactgc
     9721 tttatcagca aatgagcgcc tggtgcagct atcatacctt cgaaggtgct gccgcgttcc
     9781 tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg tctgtttgat tacctgactg
     9841 ataccggcaa tttaccgcgt attaataccg ttgaatctcc gtttgctgaa tattcctcac
     9901 ttgatgaatt attccaggaa acctataaac acgaacaatt aatcacccag aaaattaacg
     9961 aactggctca tgctgcaatg accaatcagg actacccaac atttaatttc ctgcagtggt
    10021 atgtttctga gcagcatgaa gaagagaaac tgttcaaatc gattattgat aaattaagcc
    10081 tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa agaactctct accctcgaca
    10141 cacaaaacta atgctctccg gcggcaggtt tctgccgccg tttcaaatca gtgacggcaa
    10201 atcttactct gatcggtcga aaaaccttct tctgaaggta taaatttgcc ttttgctgcc
    10261 agaaacgcta ccagctctcc cgctgtcacc ccttctgccg agcacgtgtg aaaacgtgcc
    10321 tgttcaccaa aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct
    10381 gattcaatca tcatatttaa cacttcatga ccgtgaatag agtccatcgt tcctcctcaa
    10441 aaaaagccta gcgtagcgat taccgcttat gaagactttg cgccagcgca ggactgaatg
    10501 cttttattgt acatttatat ttacacaata tgtaacgtcg gtttgacgaa gcagccgtta
    10561 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
    10621 aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
    10681 tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg
    10741 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
    10801 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
    10861 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcagcatac atcagcggtg
    10921 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
    10981 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg
    11041 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
    11101 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
    11161 tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga
    11221 gtattgtcag ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta
    11281 gtgccatccc cctggtggca tatattttct ggcaggtggc gacgcttggc agcattgatt
    11341 caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt
    11401 tacgcgaaat ggtggcctct ccgcatgttg agctggcggt gcatttattt gctgatttag
    11461 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttgt
    11521 ttcagcgttc aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc
    11581 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
    11641 tggcgctggc ggtactggca ttgattatcc cttcactatt gacctggcaa agcagaaagc
    11701 acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc
    11761 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcaggcttg ttaccagaag
    11821 tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
    11881 tgtgacctct taatgcaggc agcactgctt aaatttctta ccactgccgc acggacaagg
    11941 atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
    12001 atgcgccatc cagtatgcat gtaaatcaag cgccgccagt cgaatggcat ctacgctctc
    12061 ttcaaacgct tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag
    12121 cgcaatcgcc tccagcgctg gttttaaaga atcgggcaac gttgaccagt cagaaagtgc
    12181 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
    12241 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
    12301 atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca
    12361 acgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttctg gttcaatctc
    12421 ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc
    12481 gtggtcagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
    12541 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
    12601 gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg
    12661 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
    12721 attactttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
    12781 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
    12841 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
    12901 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac
    12961 gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgagaccc cgaccttggc
    13021 aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt
    13081 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
    13141 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa
    13201 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
    13261 gagtcagtac cgcagccaca aagaaaagtg caataccgac gtactcaacc caaatgttcg
    13321 gacgccacag cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc
    13381 aggagacggc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
    13441 taataatttc acgggcgatc atcgttgccg ccggtaatgt cacccaccag ctgtgataat
    13501 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgccaca gggtcaagga
    13561 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
    13621 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
    13681 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
    13741 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
    13801 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
    13861 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
    13921 tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt
    13981 atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc
    14041 gcgaatatgc tggataacat gcagcgcggg tgaatctggc ggcaaactaa atccctcacc
    14101 ttccggctca aagaacagcg tttccagtcc agccttacga tctgctcctt tggcaacgcc
    14161 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
    14221 ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
    14281 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
    14341 cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
    14401 caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat
    14461 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt
    14521 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
    14581 ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
    14641 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
    14701 cggtaaggtg cgcatctggc tgccttgtaa atagaactga cctacgaagg tttctaccac
    14761 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
    14821 gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat
    14881 aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc
    14941 ctgaatttgg tcgcgaatac atgccgcttc ttcaaactcc agattctggc tggcagtttc
    15001 catacggcta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
    15061 atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag
    15121 acagcgccct atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc
    15181 gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc
    15241 attcgggaac gggccgaaat attcaccttt ggcatgcttt gcaccacgat gcatcgccag
    15301 acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa
    15361 aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt
    15421 ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt
    15481 gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
    15541 gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt
    15601 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
    15661 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
    15721 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
    15781 tctgagatct cattgacctt ctggccctta gtgatcatca gcataatctg caattcacgt
    15841 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
    15901 tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
    15961 acgacttcct gcggagccgc gcctttgctg aggtagccag cagcacctgc ctgcatgact
    16021 ttcgctggta aagggttttc tgtatggacc gtaagcatga tgattttaac atcagctgtg
    16081 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
    16141 agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc
    16201 tctccggcga cttttatacc cttaatatct tccagaatgc gtcgtatccc tgcgcgcacc
    16261 agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata
    16321 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
    16381 aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg
    16441 tcacgcccgt caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg
    16501 acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat
    16561 taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt
    16621 aacgtatttt atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca
    16681 aagcttatgg tatactccgc cgccttaaca tttttcacca caaattttca ttgcaacatg
    16741 acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc
    16801 aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc
    16861 tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac
    16921 ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata
    16981 taaaaccggc tgagagcgtg cctttcagcc ggttattgac aaaatcatcg ctatctctgc
    17041 tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat
    17101 ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac
    17161 cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca
    17221 acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc
    17281 gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc
    17341 atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcacag gatcaattgc
    17401 gagaaaattt tttgcctgat aataactaac ccacgactca gggtaattgg tgtagaaagc
    17461 cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc
    17521 cagctgctga gcctgaagct ctatttcatg gtaggcctct tctgcggcct ccatcctctg
    17581 aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg
    17641 caacgcccct gagagtgagt atcatttata atgataattc aaactatcac ataaataaga
    17701 tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct
    17761 tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc
    17821 ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc agaagcttct
    17881 cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct
    17941 gctcgactat ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc
    18001 tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt
    18061 tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca
    18121 cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca
    18181 aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag
    18241 cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt
    18301 tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct
    18361 gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga
    18421 tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctacgtagca
    18481 atgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct
    18541 cgaggtcgat accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt
    18601 caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg
    18661 caacgtcgat aacacagtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt
    18721 gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg
    18781 gattgtcgcg gccagcgacg tatcttttac cagactgata aagctgttcg acagcggcgg
    18841 caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat
    18901 accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag
    18961 cgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg
    19021 atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag
    19081 cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg
    19141 cgacaggcgc atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat
    19201 actgagttgc agggtatacc cagccccttt caacaggaac ggcaaggaat caataaccag
    19261 ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc
    19321 gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga
    19381 ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg
    19441 ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag
    19501 ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct
    19561 ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac
    19621 acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg
    19681 gcgtgagttc cggcactgcc cgatgcgact accaccgacg aaatattaac cgccccttca
    19741 cactgttgcg cgatttccag cgcactctcc acataaccta gcgcgccaag agcattagaa
    19801 ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagttct
    19861 tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga
    19921 tccaacaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt
    19981 tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca
    20041 tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct
    20101 gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg ggtgacctca
    20161 tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc
    20221 ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata
    20281 acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata
    20341 aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag
    20401 caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg
    20461 catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct
    20521 gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca
    20581 gcgccgccag acgatcaacg aggatcgcat cgatacgccc tacgcgcaga tcctgatatt
    20641 tggtcgggtc atcatcatag gtacgcacat cgacaccctg aacattctgc cgcagccact
    20701 cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt
    20761 taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg
    20821 tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat
    20881 caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg
    20941 cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt
    21001 tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg
    21061 tgccgctctc tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc
    21121 ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt
    21181 tcatattcac cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca
    21241 caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg
    21301 aggatttctg cacaagtccg gtacaggttt ggcgctgata gtgttgatac ttccgtaatg
    21361 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
    21421 aaccgtcctg cagctgatcg agggaaatat tttgctcatg tagatgatta cccaaacggc
    21481 gcagacgaac gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa
    21541 atttcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
    21601 aaaatcggca caatgccgcc catgattgtt gttccgtttg ccagcctttt tcatcaagca
    21661 gcgtgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga actaaggtaa
    21721 tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa
    21781 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
    21841 caaagttaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
    21901 taatggcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
    21961 tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg
    22021 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta
    22081 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcac
    22141 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcagcatt tgacgataat
    22201 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
    22261 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
    22321 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt
    22381 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacagcatta agtaacccaa
    22441 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
    22501 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
    22561 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
    22621 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
    22681 atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag
    22741 gcattcagac gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt
    22801 tgccagcacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc
    22861 ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt
    22921 gttcaggttg gtgactgcgg aatccaaacg gttttgcacc gcacccaggg aggagcggaa
    22981 tttgtcgatg gagctgatgg cttcatccag agctttcagg ggatcggctg tcgtagctgc
    23041 ttttgtttca gcatctgtcg tcaatttacc atcagcatct ttataaactg ccttaccgct
    23101 gccgttagtc actgaaccat catcctgtag gtaaaaatct acagtatcag atgtggtcaa
    23161 cgcctcgttg ttatcaacat aaaccgaatc aacacctttt gttacagcgc cagttgtgcc
    23221 gttgacgcta taacctgcag tagtgaaatc aacattgttt cccgaggttg atacagcttt
    23281 atcgaaagct gcctgatcaa cttttgcacc tgttactgaa taagtaatag tgtccggcgt
    23341 agtactatta ccggtaaagg agatagaggt accgccaatg tcaatggttg tcgctttggt
    23401 attattggct gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag agccgttttt
    23461 cgttaagcgc ccagttgtat caatgtagag tttatctcca ttgcttgccg taattttacc
    23521 atcactgctg atctgaacat cctgtgcaga accatcgata gtaatagtgc ccttagcggt
    23581 atcaccaact cccggggtca aatatttctg aacatcggca gctgaagccg ttgtggtatc
    23641 aaaagagtat gacttacttg cactgtcata cttgtaattc gttgcactag ccgccgttcc
    23701 gaagccgtta ttaatggttg cctgaacagt atcaccatca ctaactccag ccagcaaatc
    23761 agacgcttta gcagcatcgt aacccgcact cacagtatat ttgttgccga ccactgttgc
    23821 attagcagct accaagtcag ctttagatgc agcagtatta gccacagccc cgccaccatt
    23881 cacattaaac ccattcagcc ccagcgtatc agagtcaatt ttcttcagat caatagtgat
    23941 agtctggccg tcattcgcac caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt
    24001 cacgccgttg aactgggtct gaccggatac gcggtcaatt tcgtcgagac gggatttgat
    24061 ttcgtcctga atggagtcca gatccgaatc agagttagtc ccggtagaag cctgaaccgt
    24121 cagctcacgg atacgctgta agttgttgtt gatttcggac agcgcgcctt cagtggtctg
    24181 tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt taatgttaga
    24241 agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa
    24301 gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt tgatattatt
    24361 ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc gttatcctat
    24421 attgcaagtc gttgattacg ttttgggttt ctacccgtcg gctcaatcgc cgtcaaccct
    24481 gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca ttttttgtta
    24541 gtcgacgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa caattaaacg
    24601 taaactttgc gcaattcagg ccgataaccc cggtattcgt tttacgtgtc gaaagataaa
    24661 aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg atttaagttc
    24721 aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt caaatcagca
    24781 atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt
    24841 ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca gcaccaccag
    24901 cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga aatacaccat
    24961 cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca gagatgatac
    25021 gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg acgacaaaga
    25081 tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat
    25141 caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg gtgaatatcg
    25201 tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct cggtcagcgg
    25261 tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca acggtatgga
    25321 ggtctcggtc gccgcccaga atgcgcagct gacggtcaac aacgtcgcca tcgagaacag
    25381 cagcaacact atcagcgacg cgctggaaaa catcaccctg aacctgaacg atgtcaccac
    25441 gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg cgattaaaga
    25501 ctgggtgaat gcctataact cgctaataga taccttcagc agcctgacca aatacaccgc
    25561 cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg gcgactccac
    25621 gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca gttcttccag
    25681 ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca aactggaact
    25741 ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat
    25801 tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc tgaccagttg
    25861 gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaaa ccctgaataa
    25921 gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa
    25981 agaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca gcagctactt
    26041 aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg ggagacaggt
    26101 catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat
    26161 gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt
    26221 tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa
    26281 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga
    26341 attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa
    26401 tttacgcaac gatgtctccg cagtcgaaga ggtggaagca ttaatgcgta atattgccga
    26461 tgtctggaaa gagtctttac tctccccttc ttcgattcag gacccagtct gatgaacaat
    26521 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
    26581 ctggcaacgg aagaacaatg ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg
    26641 gtgcaggaga tcgcacacct gactgaagag gttgccccgt ccactacgat gcaggagcag
    26701 ctccgcccga tgctgcacct gattctcgac aacgaaagca aggtaaagca gttattacag
    26761 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
    26821 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
    26881 ttaatgagcc gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg
    26941 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
    27001 aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta
    27061 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct
    27121 acgactccta tgatttattt gatctaggtg agtttgatca gaaaggcagc atccctacta
    27181 aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg
    27241 cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
    27301 gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg
    27361 aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg
    27421 atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta
    27481 aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta
    27541 atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga
    27601 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg
    27661 cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta caggaagttg
    27721 cgccaaagcc gctgtttatt gtggctgaat actggtcgca tgaagtcgat aaactgcaaa
    27781 cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat
    27841 tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc
    27901 tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt
    27961 tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
    28021 tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattacg
    28081 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
    28141 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc
    28201 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
    28261 tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
    28321 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgacagac gaaaacggcg
    28381 aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    28441 aaatccatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    28501 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    28561 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    28621 acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat cccccgctgc
    28681 cactgacggc aatccaaggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc
    28741 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
    28801 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    28861 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    28921 gtggcattaa agctctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
    28981 tattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcggctc
    29041 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    29101 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    29161 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
    29221 gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    29281 tccatctgga aggatcgcca ttaacccgca tcgacggcat gatgatcctc ggtatgtttg
    29341 gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc
    29401 gtatccgcat tatgcaggcc atcattggcg gcattatcgc cggttttggc gcgcgtctgg
    29461 caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct
    29521 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
    29581 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
    29641 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
    29701 gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg
    29761 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    29821 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
    29881 ccatcgggat cttcagttac gtacagttag gcgttgaacc taaaatcatg tgggcgggac
    29941 caaatgcggt aattggtggt ttactttttg gttttggcat cgtgctggca ggcggctgcg
    30001 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    30061 gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
    30121 ccaccgactg ggacaaaatc aacctgctga aaacttttgg tccgatggga ggcctgctgg
    30181 tgacatattt gctgctgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct
    30241 tcccccgtga ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
    30301 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
    30361 gccgcaatta aaaaaaggtg agatcctgga agtggtgagc gactgtccgc agtcgatcaa
    30421 taatattcca ctggatgcgc gtaatcacgg ctacacggtg ctggatattc agcaagacgg
    30481 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    30541 gaacatcgtt acctctgaca catagatttt acccgccccc tctcctattc ttttcagtat
    30601 gatctcagga ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc
    30661 ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca
    30721 gatacaatgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc
    30781 atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta
    30841 acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa accgctctgg caacatggtc
    30901 gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc
    30961 ttattttatc tatcgcgctg acggacaacc tgtttttatg gccgcgatag gtagcatacc
    31021 gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg
    31081 tctggtagat attcatgacc gacgcccgct ggttctgtcg ccagaagctg cacgggaatg
    31141 gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc
    31201 agcaaaccag ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg
    31261 cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaatgac gaggacccct
    31321 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg
    31381 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc
    31441 catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca
    31501 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    31561 gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg cggcgcattt
    31621 cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg acattgcgcc
    31681 ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc aaatcctcaa
    31741 tgactatcat tacgtggttt ttgctgacgg tcgttgtgac tttgtgaagg tagtccctgc
    31801 ggatatttgc gatacaggaa tgcagtcgct gtattttgcg tttctgcttc tgccagttgt
    31861 tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcttc ttctggtttt
    31921 tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgaggca agtttagcca
    31981 cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat acttcacttt
    32041 ctgcctgaat actgatgtac cacttaccgc aggactggct gacagtgaca tttttcacaa
    32101 cacccgtgac ctgacggctg ttccggtagc gcatccagcc aagttttggc agaaaaatac
    32161 ggctgttttc ctgagcgagc ttaacgccct gcggatagcg gaatgcatca ttctgtcacc
    32221 gctttttgaa tcaggggaac gccgagcgct tctggaaaaa gtttttgcag gcctgctcag
    32281 ggtctttcag tgactgttgc aatggctgtg acggagcatc tttaatccat tgcgtttcag
    32341 tggcattttt ccactcaacc aatcaggaag ccattttcgc gtaagggatg tctttgttac
    32401 cggccttatg attctcattc tgacgtgcca gagtacgact gaaaacgaaa cgacatgcgc
    32461 ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg tcttaaccgg aatttgaata
    32521 cctgtagtcg tttcacaggg caaattatac tttggtctgt gaaaaaaact gatatccgac
    32581 gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt ctttgttacc aa
//
LOCUS       DAJTNW010000041        32624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNW010000041 DAJTNW010000000
VERSION     DAJTNW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..946)
                     /locus_tag="OO179_002002"
     CDS             complement(<1..946)
                     /locus_tag="OO179_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5532072.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAG"
     gene            1192..1818
                     /gene="gstB"
                     /locus_tag="OO179_002003"
     CDS             1192..1818
                     /gene="gstB"
                     /locus_tag="OO179_002003"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5532073.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1815..2929)
                     /gene="yliI"
                     /locus_tag="OO179_002004"
                     /pseudo
     CDS             complement(1815..2929)
                     /gene="yliI"
                     /locus_tag="OO179_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3040..3423)
                     /gene="bssR"
                     /locus_tag="OO179_002005"
     CDS             complement(3040..3423)
                     /gene="bssR"
                     /locus_tag="OO179_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5532074.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3636..4961
                     /gene="rimO"
                     /locus_tag="OO179_002006"
     CDS             3636..4961
                     /gene="rimO"
                     /locus_tag="OO179_002006"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5532075.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5008..6336)
                     /locus_tag="OO179_002007"
     CDS             complement(5008..6336)
                     /locus_tag="OO179_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5532076.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6344..8692)
                     /locus_tag="OO179_002008"
     CDS             complement(6344..8692)
                     /locus_tag="OO179_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5532077.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8870..9781)
                     /gene="gsiD"
                     /locus_tag="OO179_002009"
     CDS             complement(8870..9781)
                     /gene="gsiD"
                     /locus_tag="OO179_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5532078.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9784..10704)
                     /gene="gsiC"
                     /locus_tag="OO179_002010"
     CDS             complement(9784..10704)
                     /gene="gsiC"
                     /locus_tag="OO179_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5532079.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10722..12260)
                     /gene="gsiB"
                     /locus_tag="OO179_002011"
     CDS             complement(10722..12260)
                     /gene="gsiB"
                     /locus_tag="OO179_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5532080.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12280..14151)
                     /gene="gsiA"
                     /locus_tag="OO179_002012"
     CDS             complement(12280..14151)
                     /gene="gsiA"
                     /locus_tag="OO179_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5532081.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14138..15103)
                     /gene="iaaA"
                     /locus_tag="OO179_002013"
     CDS             complement(14138..15103)
                     /gene="iaaA"
                     /locus_tag="OO179_002013"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5532082.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15307..16542
                     /gene="moeA"
                     /locus_tag="OO179_002014"
     CDS             15307..16542
                     /gene="moeA"
                     /locus_tag="OO179_002014"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5532083.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16542..17291
                     /gene="moeB"
                     /locus_tag="OO179_002015"
     CDS             16542..17291
                     /gene="moeB"
                     /locus_tag="OO179_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5532084.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17470..18132)
                     /gene="fsa"
                     /locus_tag="OO179_002016"
     CDS             complement(17470..18132)
                     /gene="fsa"
                     /locus_tag="OO179_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5532085.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18263..19162
                     /locus_tag="OO179_002017"
     CDS             18263..19162
                     /locus_tag="OO179_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5532086.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19168..21600
                     /locus_tag="OO179_002018"
     CDS             19168..21600
                     /locus_tag="OO179_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5532087.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21746..22561
                     /gene="ybiV"
                     /locus_tag="OO179_002019"
     CDS             21746..22561
                     /gene="ybiV"
                     /locus_tag="OO179_002019"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5532088.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22713..23978
                     /locus_tag="OO179_002020"
     CDS             22713..23978
                     /locus_tag="OO179_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5532089.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24219..25811)
                     /locus_tag="OO179_002021"
     CDS             complement(24219..25811)
                     /locus_tag="OO179_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5532090.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26030..26950
                     /gene="ldtB"
                     /locus_tag="OO179_002022"
     CDS             26030..26950
                     /gene="ldtB"
                     /locus_tag="OO179_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5532091.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27008..28126)
                     /locus_tag="OO179_002023"
     CDS             complement(27008..28126)
                     /locus_tag="OO179_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5532092.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28123..28590)
                     /gene="mntR"
                     /locus_tag="OO179_002024"
     CDS             complement(28123..28590)
                     /gene="mntR"
                     /locus_tag="OO179_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5532093.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28776..28904
                     /gene="mntS"
                     /locus_tag="OO179_002025"
     CDS             28776..28904
                     /gene="mntS"
                     /locus_tag="OO179_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5532094.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29176..30759
                     /gene="opgE"
                     /locus_tag="OO179_002026"
     CDS             29176..30759
                     /gene="opgE"
                     /locus_tag="OO179_002026"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5532095.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30807..31322)
                     /gene="ompX"
                     /locus_tag="OO179_002027"
     CDS             complement(30807..31322)
                     /gene="ompX"
                     /locus_tag="OO179_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5532096.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31375..31440)
                     /locus_tag="OO179_002028"
     CDS             complement(31375..31440)
                     /locus_tag="OO179_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532097.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31675..32562
                     /gene="rhtA"
                     /locus_tag="OO179_002029"
     CDS             31675..32562
                     /gene="rhtA"
                     /locus_tag="OO179_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5532098.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8166 a         8696 c         7881 g         7881 t
ORIGIN      
        1 cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga gtcacaaagg
       61 tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag gtcaataatt
      121 tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac
      181 gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc gtggttcctg
      241 tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg ttaggctgac
      301 gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt
      361 ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat
      421 ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg gcataaccat
      481 tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc gcaatacagg
      541 cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc
      601 ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat
      661 ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa
      721 ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca tccgcattgc
      781 cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc
      841 tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg
      901 cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag
      961 tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt aataacgcac
     1021 acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca atatcctgcc
     1081 gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta actttacttc
     1141 accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag tatgattacg
     1201 ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct cgaagaactg
     1261 gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca cgatgctgat
     1321 tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga aagtgatctc
     1381 attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc
     1441 ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac
     1501 cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac accaccggaa
     1561 gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct gtttgccctg
     1621 ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat
     1681 atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg gacaccgcgt
     1741 ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg
     1801 atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact ggactcgtcg
     1861 gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg
     1921 tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca
     1981 tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat ttatcgctgt
     2041 tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg
     2101 tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc
     2161 aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac
     2221 cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc gccattcctt
     2281 gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc tttataaaag
     2341 gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat
     2401 ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt
     2461 taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc
     2521 ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg ccataaccca
     2581 cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag atgcggcgag
     2641 actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg tgcgcccaaa
     2701 cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag tgacgcaact
     2761 cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc agtgcccagg
     2821 gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc gcccagagag
     2881 cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata acgcctccgg
     2941 attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt ttacaaaaac
     3001 ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct caagcatggc
     3061 gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt caagcttatc
     3121 ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac atactgcggc
     3181 tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat ccagatgaag
     3241 attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt tatctcgtag
     3301 atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca cgtatgcagc
     3361 gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc tgtcaacgaa
     3421 catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc tattttttga
     3481 gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac atttccttcg
     3541 cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta gaatccctgc
     3601 cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact ccccagccga
     3661 aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag cgtattctca
     3721 ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg aacatggtga
     3781 tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa gccattggtg
     3841 aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca aaagaagatc
     3901 agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc tatgagcagg
     3961 ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc ctgagcctgg
     4021 tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa atttcggaag
     4081 gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac ctggtgagcc
     4141 gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc gttaaagaga
     4201 ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat cgtactggct
     4261 tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag ttatcgaaac
     4321 tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac gacgtcatcc
     4381 cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag cacgccagcc
     4441 cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg gcgcgcatca
     4501 aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt gtcggcttcc
     4561 ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa gcacgtctgg
     4621 atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat gccctgcctg
     4681 accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag ctgcagcagc
     4741 agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg attatcgacg
     4801 aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa atcgacggcg
     4861 cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt gtgaaagtcg
     4921 agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag gccgggcaaa
     4981 atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc tgccgttttt
     5041 cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg tagtacattc
     5101 atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct ggaaatgaac
     5161 gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa tcaccgcaaa
     5221 ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg atttaaggat
     5281 tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt cgttaatgtt
     5341 tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca gcgcttttaa
     5401 atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga acgccgagtt
     5461 atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc catccagtac
     5521 gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat taatcactct
     5581 gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg caaagaaaat
     5641 aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt acaatccggt
     5701 cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt ttttagcatt
     5761 atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca ggttctcgct
     5821 gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca aaaatatcag
     5881 actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt caaaagagat
     5941 aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg cacaataatt
     6001 tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag gaccgacatc
     6061 gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa ccaacagctg
     6121 ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc gatattcagc
     6181 aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat acacccgttc
     6241 ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa aaatcagcag
     6301 actgactgta agtacgaact tattgattct ggacatacgt aaactactct tttactaatt
     6361 ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac ccctgagcct
     6421 gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg ccttccgcaa
     6481 tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt tttcccgcag
     6541 taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga ttaatttcat
     6601 aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc cgtacccctt
     6661 gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg atcacggttt
     6721 cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg atggtggtaa
     6781 aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata tgcagtaaga
     6841 aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt gccgttttaa
     6901 taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta atgaacttca
     6961 gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc gcgctcagaa
     7021 tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg ccaatttcca
     7081 ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg agtttcgcgt
     7141 catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc gccagtttca
     7201 gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg gcagacttat
     7261 ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc gctattttac
     7321 gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca ttgaatgtca
     7381 cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc ctgacgattt
     7441 tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac agtttgatga
     7501 tccccacagc cagatactga ttattgttgg actcaataat cgccatattt ttaataaaac
     7561 cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca atttcggcat
     7621 ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg ccttcgcgaa
     7681 ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta ttaaccagct
     7741 cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat accaccagca
     7801 acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg ttcaataaat
     7861 taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact gctgatgtct
     7921 ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg ctattataaa
     7981 taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct tgtttcaaaa
     8041 atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac cgccagttta
     8101 aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg atacgtagcg
     8161 taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga ttccagctgt
     8221 tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa tcggcaaaaa
     8281 taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga taaacaacga
     8341 ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt accagtccca
     8401 ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga atctcctctg
     8461 tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca atatctttaa
     8521 agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt tttaccgcgc
     8581 gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac agtgctgcca
     8641 ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc atgttaatac
     8701 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgcggct
     8761 ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact tgcttagggc
     8821 aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac tatcctttaa
     8881 ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca
     8941 gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata tccgctcgtg
     9001 cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg ccgagaaatg
     9061 acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag
     9121 aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg gcaccaatac
     9181 tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccaggc
     9241 gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc
     9301 ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc
     9361 aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac aagcccagca
     9421 acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg
     9481 caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca aaccagtgct
     9541 gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg tcatagggag
     9601 cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat gcggcggtca
     9661 tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgtacctggt
     9721 caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca
     9781 tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt
     9841 gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg
     9901 catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac
     9961 cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat
    10021 catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct
    10081 cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg taaagcgcgc
    10141 catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact
    10201 gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa ttaaaaacat
    10261 ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc
    10321 cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc
    10381 ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg caatctcatc
    10441 ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat
    10501 atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc
    10561 tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat
    10621 gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca accccagtaa
    10681 tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa atccgcgtct
    10741 tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc
    10801 gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat gatatcctgc
    10861 gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc ctgagccagg
    10921 aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg tggccagttt
    10981 tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc cgaccagcca
    11041 gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc tgccgcccgc
    11101 tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa ctgctgctgg
    11161 gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtactg
    11221 aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc
    11281 cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt agctggcgtt
    11341 gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta attcagcgcc
    11401 tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat ataacgctgc
    11461 ataattgacg gactggccat caactcgata tttttgtttt tctccagcag tgtggcctgc
    11521 tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg
    11581 ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc tggctgccag
    11641 taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac
    11701 ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgtcggt
    11761 gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg
    11821 agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt cttatacagg
    11881 ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc ttttaccgcc
    11941 actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtaa
    12001 gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct
    12061 ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca
    12121 ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac cacatctttg
    12181 gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac taacccacta
    12241 cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta
    12301 ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc
    12361 cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg
    12421 cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg
    12481 atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt gcccgagata
    12541 catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg agataaacag
    12601 atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat
    12661 agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat tcaatgccaa
    12721 cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca
    12781 ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcgtc
    12841 tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg
    12901 acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc
    12961 ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa tttcgccgcc
    13021 ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc
    13081 acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg
    13141 cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgacaagatt
    13201 acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggttcctg
    13261 tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta accctttcat
    13321 cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg
    13381 aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg
    13441 atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat
    13501 ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc
    13561 ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat
    13621 cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa tggtttgtgc
    13681 ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact
    13741 ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa atactgggtt
    13801 cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca catggcgcat
    13861 ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc gcaacagcat
    13921 tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg ccagcgcagt
    13981 cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc gttgcagact
    14041 aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattaag
    14101 attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca acggtgtccc
    14161 ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca
    14221 ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg attaagccac
    14281 cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac
    14341 ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga
    14401 cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggcac
    14461 ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg cctgtggacg
    14521 tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg cccattttct
    14581 gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc tcgcgcgctg
    14641 ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg cgctccatgc
    14701 catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct
    14761 ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc gccaccgcac
    14821 cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt tcatcacgcg
    14881 taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga cgcaccgctt
    14941 ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg gtttcaacaa
    15001 tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc gcgcggctaa
    15061 ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc gtccattaag
    15121 ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt
    15181 gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc
    15241 atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct ccgcaggagt
    15301 gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt
    15361 tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg ttttggtcgt
    15421 attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa ctccgctatg
    15481 gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc cgttgccggt
    15541 aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg cattcgtatt
    15601 atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa
    15661 caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt
    15721 cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg cctgactacc
    15781 gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg
    15841 cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac
    15901 ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca gctgggttgc
    15961 gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc cgcatttatt
    16021 gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt gggtgaagcg
    16081 gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt
    16141 aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg cggcctgccg
    16201 ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaaa
    16261 ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc
    16321 ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg caacgccgac
    16381 ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag ctcctttagt
    16441 ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt gggcgaatgg
    16501 gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc
    16561 aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg
    16621 aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt
    16681 cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gacacggttt
    16741 cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg caaccgaagg
    16801 tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt acgccagtca
    16861 atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat ctggtgctcg
    16921 actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg
    16981 tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc ttcacttatc
    17041 aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcgttaacct
    17101 gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg caggcaatgg
    17161 aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc gtcatgtacg
    17221 atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt
    17281 gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc atcaggcatt
    17341 tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg ggcacagatt
    17401 tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat gaggatgcag
    17461 aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct caaacttcgt
    17521 cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca gtggcagagt
    17581 aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc
    17641 tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt cggtcacagt
    17701 ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa catattccgc
    17761 acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc ccagcgtcgg
    17821 aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca ccggaacttt
    17881 caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca tcccttcagc
    17941 agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt catgaagttg
    18001 cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt tagtggtcac
    18061 acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg aagtatccag
    18121 atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac tatcacggga
    18181 aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta ttttaatgtt
    18241 tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga cccatgatgg
    18301 ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc gctggtgtca
    18361 gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac tgtgtctgga
    18421 aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc tgaatggttt
    18481 gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg actgctgtcc
    18541 gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca tggcgaccgt
    18601 tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt cgggtggtga
    18661 acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg aggcaggcat
    18721 tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc
    18781 ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg
    18841 gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg cagcgggcaa
    18901 aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa cctctgtaaa
    18961 agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt ttctgcccta
    19021 ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg ccccggaaaa
    19081 accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc agaaagggtt
    19141 aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac gctcagcgac
    19201 cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt ctgtaccgag
    19261 cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat cccggtacgt
    19321 cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa acacgatgag
    19381 ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact
    19441 gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc tggctttgcg
    19501 gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg cggtcagacg
    19561 gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct ggcgaccggc
    19621 atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt caatttcccg
    19681 ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc
    19741 atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc gattgatatc
    19801 gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg tgcaatggcc
    19861 gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga aaactgcgat
    19921 cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg ttacttcatc
    19981 cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg tatggatcag
    20041 tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga tcgcgaacac
    20101 gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa aatccgctcc
    20161 ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac cattggcgga
    20221 caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta cgcgatcctc
    20281 gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta ccatgcagga
    20341 atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt cgggatgccg
    20401 gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat tgaaccgcag
    20461 gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg caaatggggc
    20521 tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa
    20581 ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca
    20641 ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat ccgttactac
    20701 acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac
    20761 gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa
    20821 ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa cctcggcaac
    20881 agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca gcaacagctg
    20941 gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg tcagcggctg
    21001 attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct gctggctcgc
    21061 gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta cggtcgtggt
    21121 ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt accgtttggc
    21181 gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc agaaggcgct
    21241 agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc agtgggtaaa
    21301 ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg
    21361 ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt ctttgaagtg
    21421 cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa
    21481 aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc cgccttcttc
    21541 accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca tatgctgtaa
    21601 tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca atttcatcct
    21661 tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac tgttacggtt
    21721 tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag atatggacgg
    21781 tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc aatatcagga
    21841 actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt accagcttat
    21901 ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg gcgcactggt
    21961 ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat cgcggattgt
    22021 tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc tgcaaagtgc
    22081 atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac actaccatcg
    22141 cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt tttcgctcaa
    22201 cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc tggatggcat
    22261 tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg gtctacataa
    22321 agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc
    22381 gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt cctttgcgat
    22441 gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg ataataatca
    22501 tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt ttaacagctg
    22561 acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct gtacatcaca
    22621 tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg caaattagca
    22681 tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc agcgacacat
    22741 tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg
    22801 gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa
    22861 tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca
    22921 aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga
    22981 taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc
    23041 tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc
    23101 tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc
    23161 gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa
    23221 gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc
    23281 gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc ggggcgctgg
    23341 aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat ggcaatctgg
    23401 cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca
    23461 acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc tcggatatgc
    23521 tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg tacgtactgt
    23581 tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag
    23641 tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa
    23701 aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg
    23761 ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga
    23821 aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg
    23881 acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca
    23941 acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat
    24001 gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat
    24061 gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc aatatattac
    24121 aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt gtcatcagcc
    24181 gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc tttactacgc
    24241 aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat ttccagaatg
    24301 cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga
    24361 tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg gttggtcggt
    24421 tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag catccgccct
    24481 ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct gaacagcaaa
    24541 cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc
    24601 cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc gtagtaacca
    24661 atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc gcccaccagc
    24721 gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag tttttcaccg
    24781 acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc tttggtcaga
    24841 ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg gataaacggg
    24901 ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc
    24961 gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac
    25021 tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg cgcctgggtc
    25081 gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc gtaatccaga
    25141 tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat gatgatcatg
    25201 gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg
    25261 ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg cgccagaagc
    25321 acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg ttccactgga
    25381 attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc gtccatttcg
    25441 ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat ttccggcaaa
    25501 gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc catgatcacc
    25561 gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt accaatgcgc
    25621 tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc gagaatcttc
    25681 ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg
    25741 aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt gacgttactg
    25801 gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta tgccataagt
    25861 ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc aaaaaatgtg
    25921 atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg cttccaatac
    25981 tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa
    26041 attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc
    26101 ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac
    26161 cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc agatggggct
    26221 ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag gcggtactgt
    26281 actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca tcgtcattaa
    26341 cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta tcgtgctgcc
    26401 gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca aagttgagca
    26461 taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt accgcgctgc
    26521 gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg gtctgtatgc
    26581 actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg gtatcggcct
    26641 gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa
    26701 agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga ccaccgagcc
    26761 agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag cccagtttga
    26821 aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga ccggtcagcc
    26881 agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga tgccggttcg
    26941 cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct tttttatttg
    27001 ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc gccgcccaca
    27061 acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg ttcgccatac
    27121 gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc acgtttaccg
    27181 cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta ctcggcacgt
    27241 tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca gataaatgac
    27301 tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa
    27361 acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg cgacgcgcca
    27421 gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg aactccagcg
    27481 ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt ttccactccg
    27541 gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac cagcacaaca
    27601 gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata aatccggcaa
    27661 acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt ggcgtcagta
    27721 gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc gggatctcac
    27781 acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca tcgttggtca
    27841 gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga cgctccgtag
    27901 caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac tccacacctt
    27961 tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc
    28021 aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga attaataact
    28081 gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt
    28141 ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct ccatgccttc
    28201 cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat tttcgactat
    28261 ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta aaaacacgcc
    28321 acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca ttttagccac
    28381 cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt cccccacttc
    28441 cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc gatgcgcctc
    28501 tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg tcactttttt
    28561 tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg acgcatacat
    28621 agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa aaaattaacg
    28681 ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc aatcatccct
    28741 caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag aggtgtatgc
    28801 gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg ttgtgcgata
    28861 tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt cgcggtacgc
    28921 cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa accacttttt
    28981 gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg
    29041 ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg acccctttcc
    29101 cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac atggcgtttg
    29161 ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg tagccgggta
    29221 tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct cggcttaggt
    29281 taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt gctatggcga
    29341 acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc ggcttgttat
    29401 ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc attgcactcc
    29461 accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag ctacctggtc
    29521 ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa
    29581 gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac attttttgtt
    29641 gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac tggctatccg
    29701 gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga agagcaagaa
    29761 cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt taagccgaag
    29821 tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct cggtgccttt
    29881 ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat atttgctgac
    29941 tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa tcgcgtagtc
    30001 gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc gggcttccag
    30061 acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat cgccagcatc
    30121 gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc agataaaaac
    30181 acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa
    30241 ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga caggacacaa
    30301 ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg
    30361 caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg cagcagcttc
    30421 tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa
    30481 taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat ggtcatttcc
    30541 agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga cttcttaggc
    30601 tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata cccgtttata
    30661 tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa ggtggattac
    30721 aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc cgccccgaga
    30781 ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc aatccaagtg
    30841 cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc aacgttttcc
    30901 atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg
    30961 taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac accgtagatg
    31021 cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta ctggtttttg
    31081 ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga accgatcaca
    31141 cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc
    31201 atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg
    31261 gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc aatttttttc
    31321 ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca aaaattaatg
    31381 ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga acgtttccat
    31441 acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag tattacgcat
    31501 gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt ccatcaatcc
    31561 tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac cagcattggg
    31621 tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga aaggatgcct
    31681 ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt tgccatggcg
    31741 tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag cgcaccgggt
    31801 gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt taagccatgg
    31861 cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt
    31921 gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat tgcggtggcg
    31981 ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc ggtagatttc
    32041 gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg gcaagacgtt
    32101 tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg ttgggctatt
    32161 tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt agcaattggt
    32221 tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg tgaagcactc
    32281 tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac cgctctgcct
    32341 tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg tacgctgatg
    32401 agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga aacactgaca
    32461 cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc tacgctgaca
    32521 gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat ttctgtatgg
    32581 ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DAJTNW010000042        32142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNW010000042 DAJTNW010000000
VERSION     DAJTNW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO179_002030"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO179_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO179_002031"
     CDS             1127..1234
                     /locus_tag="OO179_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532099.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO179_002032"
     CDS             complement(1287..2045)
                     /locus_tag="OO179_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5532100.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO179_002033"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO179_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5532101.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO179_002034"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO179_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5532102.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO179_002035"
     CDS             3758..4618
                     /locus_tag="OO179_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5532103.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO179_002036"
     CDS             complement(4659..5195)
                     /locus_tag="OO179_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5532104.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO179_002037"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO179_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5532105.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO179_002038"
     CDS             6173..6763
                     /locus_tag="OO179_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5532106.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="OO179_002039"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="OO179_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8290..9106)
                     /locus_tag="OO179_002040"
                     /pseudo
     CDS             complement(8290..9106)
                     /locus_tag="OO179_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="OO179_002041"
     CDS             complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="OO179_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5532107.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9829..12090
                     /gene="katE"
                     /locus_tag="OO179_002042"
     CDS             9829..12090
                     /gene="katE"
                     /locus_tag="OO179_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5532108.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="OO179_002043"
     CDS             complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="OO179_002043"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5532109.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12908..14260)
                     /gene="celF"
                     /locus_tag="OO179_002044"
                     /gene_synonym="chbF"
     CDS             complement(12908..14260)
                     /gene="celF"
                     /locus_tag="OO179_002044"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5532110.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="OO179_002045"
                     /pseudo
     CDS             complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="OO179_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="OO179_002046"
     CDS             complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="OO179_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5532111.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="OO179_002047"
     CDS             complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="OO179_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5532112.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="OO179_002048"
     CDS             complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="OO179_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5532113.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="OO179_002049"
     CDS             complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="OO179_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5532114.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18217..19044
                     /gene="nadE"
                     /locus_tag="OO179_002050"
     CDS             18217..19044
                     /gene="nadE"
                     /locus_tag="OO179_002050"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5532115.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19274..20161
                     /gene="cho"
                     /locus_tag="OO179_002051"
     CDS             19274..20161
                     /gene="cho"
                     /locus_tag="OO179_002051"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5532116.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20121..20696)
                     /gene="ves"
                     /locus_tag="OO179_002052"
     CDS             complement(20121..20696)
                     /gene="ves"
                     /locus_tag="OO179_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5532117.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20899..21384)
                     /gene="spy"
                     /locus_tag="OO179_002053"
     CDS             complement(20899..21384)
                     /gene="spy"
                     /locus_tag="OO179_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5532118.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21714..22682)
                     /gene="astE"
                     /locus_tag="OO179_002054"
     CDS             complement(21714..22682)
                     /gene="astE"
                     /locus_tag="OO179_002054"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5532119.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22675..24008)
                     /gene="astB"
                     /locus_tag="OO179_002055"
                     /pseudo
     CDS             complement(22675..24008)
                     /gene="astB"
                     /locus_tag="OO179_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24005..25483)
                     /gene="astD"
                     /locus_tag="OO179_002056"
     CDS             complement(24005..25483)
                     /gene="astD"
                     /locus_tag="OO179_002056"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5532120.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25480..26514)
                     /gene="astA"
                     /locus_tag="OO179_002057"
     CDS             complement(25480..26514)
                     /gene="astA"
                     /locus_tag="OO179_002057"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5532121.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26511..27731)
                     /gene="astC"
                     /locus_tag="OO179_002058"
                     /pseudo
     CDS             complement(26511..27731)
                     /gene="astC"
                     /locus_tag="OO179_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28177..28983
                     /gene="xthA"
                     /locus_tag="OO179_002059"
     CDS             28177..28983
                     /gene="xthA"
                     /locus_tag="OO179_002059"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5532122.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29150..29858
                     /locus_tag="OO179_002060"
                     /pseudo
     CDS             29150..29858
                     /locus_tag="OO179_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29863..30540
                     /gene="ydjY"
                     /locus_tag="OO179_002061"
     CDS             29863..30540
                     /gene="ydjY"
                     /locus_tag="OO179_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5532123.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30555..31262
                     /locus_tag="OO179_002062"
     CDS             30555..31262
                     /locus_tag="OO179_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5532124.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31262..31810
                     /locus_tag="OO179_002063"
     CDS             31262..31810
                     /locus_tag="OO179_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5532125.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31820..>32142
                     /locus_tag="OO179_002064"
     CDS             31820..>32142
                     /locus_tag="OO179_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5532126.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8246 a         8243 c         7612 g         8041 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttccaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaatcatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat
     8401 gtccaactta tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac
     8461 ctcatacccg agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac
     8521 accacttccc attcccggaa gatttagtgg attaaaaaga agattgatat taagcattga
     8581 tattatcgaa ggaaacttta ggagactctc attttttttg aatcgcagca aaaatgctcc
     8641 cggcaggatg tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc
     8701 tattttttat attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca
     8761 tcccaacaaa attctcatta atatctctta tccgaaactc actgccataa agattattaa
     8821 ttatcactct atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc
     8881 ctagctttga ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg
     8941 actctgctat ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca
     9001 tggtagactc aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac
     9061 cataattact ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat
     9121 tattatctta tttaaatcta agtcatttac aatataagtt taagagcgac gccacaggat
     9181 gaactatcaa aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaat
     9241 aattaaagaa aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta
     9301 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc
     9361 aaaaccggga ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
     9421 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
     9481 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
     9541 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
     9601 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
     9661 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
     9721 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
     9781 tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat
     9841 aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
     9901 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
     9961 ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa
    10021 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
    10081 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
    10141 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
    10201 cgtattgttc atgcacgcgg ctcagcagct cacggttatt tccagccata taaaagctta
    10261 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
    10321 aggttctcta ccgttcaggg tggagctggc tctgccgata ccgtgcgtga tatccgtggt
    10381 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
    10441 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
    10501 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
    10561 ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
    10621 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
    10681 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
    10741 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
    10801 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
    10861 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
    10921 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
    10981 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
    11041 gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
    11101 gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag
    11161 cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg
    11221 atcaacgata actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca
    11281 taccaagagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat
    11341 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
    11401 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
    11461 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
    11521 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
    11581 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg
    11641 attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccatgaag
    11701 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
    11761 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
    11821 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat
    11881 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
    11941 aaagcaacaa tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc
    12001 gacggtagtt ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc
    12061 attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt
    12121 gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa
    12181 ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt
    12241 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag
    12301 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
    12361 caccataaaa cgcgctactg aatccctgcg aactgcgcag attaaccggt aaatcaccag
    12421 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
    12481 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
    12541 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
    12601 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
    12661 cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc
    12721 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
    12781 tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
    12841 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
    12901 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
    12961 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
    13021 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
    13081 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
    13141 gcgtaatgcg cggatgtggc gtcgcgccat cgtgccccag cttacaggtc atttctaccg
    13201 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
    13261 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
    13321 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa
    13381 aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc
    13441 ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca
    13501 acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc
    13561 cagatacttt taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc
    13621 catttaccag cacatcctta atgaacacca tatggttgag gccaaacaaa tcgatagata
    13681 aatcatcgct gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa
    13741 tattacacac gccgataaaa cgtttaaatc cggtatgacg ataaacggct tcagtaacca
    13801 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
    13861 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt
    13921 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg
    13981 gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
    14041 aatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
    14101 gatcgaaaat aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc
    14161 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
    14221 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
    14281 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
    14341 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatatct
    14401 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
    14461 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gtttttttgc
    14521 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
    14581 cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
    14641 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actacggtac
    14701 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
    14761 catctcaaca aactcttcca gccctgtttc gcggaaattt aatgaagaaa tcactgtttc
    14821 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcaacaa agcaataagg
    14881 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg
    14941 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
    15001 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
    15061 tagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata
    15121 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
    15181 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
    15241 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
    15301 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
    15361 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gctttcgctg
    15421 cggcaaaatc gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc
    15481 cagagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
    15541 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
    15601 ccgtaccggc atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta
    15661 tcaatcgcat tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
    15721 atgccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
    15781 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
    15841 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
    15901 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
    15961 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
    16021 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
    16081 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
    16141 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
    16201 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc
    16261 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
    16321 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
    16381 gaaagaataa taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg
    16441 ctgtcgggca gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
    16501 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
    16561 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
    16621 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
    16681 gccattaaag acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga
    16741 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac
    16801 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt
    16861 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
    16921 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
    16981 tacccttaaa aaccgcaatt taaatattac ggtattgatt tatgaaataa ctctttgacg
    17041 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
    17101 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
    17161 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
    17221 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
    17281 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga gatagacatg
    17341 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
    17401 atctgtcagc cagacactcc gcaagccttg acctgcttcc atgctctggg taacttgcga
    17461 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
    17521 tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc
    17581 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
    17641 ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt
    17701 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
    17761 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
    17821 gcgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
    17881 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg
    17941 atcataagct gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
    18001 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
    18061 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
    18121 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
    18181 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
    18241 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
    18301 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
    18361 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
    18421 caggaaactc gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
    18481 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
    18541 gttaatatca agggcgcggt attggctagc gagcaggcat tgcgggaagc aggcattgaa
    18601 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
    18661 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
    18721 ggattcttca ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac
    18781 aaacgccagg gtaaacagtt actgacggca ttaggttgcc cggaacacct ttataagaaa
    18841 gccccaactg ccgatcttga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
    18901 gtgacctatg acaatatcga cgactatctg gaagggaaaa acctacctga acaggtcgcc
    18961 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaacgtt
    19021 ttcgatgatt tctggaaaaa gtaataattt gcacctattg gattgtgcga aaaagagtaa
    19081 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
    19141 atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg
    19201 tcatcaaccc gcctcttttt taatcgcctc ccgcctgtta cactggatag ataaccagca
    19261 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
    19321 aatttatgaa tatcccgaac atttacattc attccttaat gacttaccca cccgaccagg
    19381 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
    19441 tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca
    19501 atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc
    19561 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
    19621 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
    19681 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
    19741 gcagagcatc gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag
    19801 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
    19861 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgagtggt
    19921 gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata
    19981 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
    20041 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
    20101 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
    20161 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
    20221 aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta
    20281 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
    20341 actttcgcct tacagacatc caggcgcgtc ataatattga aatccatcga catctgccct
    20401 gccgtcagtt tcgcttttac cacctggtcc gccgtaaagg caaaaggctg taacggtttt
    20461 aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
    20521 actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
    20581 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
    20641 ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
    20701 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatgat tatcggcaac
    20761 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag
    20821 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
    20881 agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
    20941 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat
    21001 gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
    21061 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
    21121 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
    21181 gatttcgtgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
    21241 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc
    21301 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
    21361 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
    21421 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
    21481 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
    21541 aagaaaattt gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta
    21601 tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
    21661 taatttataa gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
    21721 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac
    21781 acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt
    21841 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
    21901 gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg
    21961 acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga
    22021 tcgttttgcc caaagggcaa cgctttgcca agttccaacg tacagaccag cgcgccaaaa
    22081 tgtctgacgc tgaaatgggt aaacgtacca ccaggctcct gatggaacac cagcgcctcc
    22141 agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
    22201 aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccaa
    22261 cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc
    22321 cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcggttcata
    22381 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc
    22441 aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt
    22501 tccacaggtg ccgtctcatt accgtggatc cccgcagaaa tcaccagcgc gccttgcggt
    22561 ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg
    22621 atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc
    22681 attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
    22741 tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt
    22801 aagacgatcg cggtagtaac gatccaccca gtcattgagc acattaaaca aggtatcgtt
    22861 catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaataccc cccgcaaccg
    22921 caggcacgcc gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc
    22981 actaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgctc
    23041 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct
    23101 gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat
    23161 cgccataaaa ccgttcaccc gcgtgccagt aactgtgact ggcgagcgaa cgcctgttgg
    23221 tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgctctgg
    23281 tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattaagc
    23341 cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta
    23401 tcattgcctt cttctcgccc gtagacaaaa agtagcatac ccggttcacc gtaatgaccg
    23461 ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc
    23521 gaatggacgc taaatttctc ttcgtcctta aaaatcgctt ttaacagcga ttcagtgacg
    23581 ggcgcttcca gcgaacggtg aaatttattg ttcaggttgg caacggtgag atgcactttg
    23641 ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa
    23701 gcggaactga cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc
    23761 tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc
    23821 gggatcacgg cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt
    23881 tttgccgcca gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca
    23941 aacgacaggc ccgcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag
    24001 gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtg gcgggcaatg
    24061 ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg
    24121 cgctggggcg atggttaccg gaagcaccaa tgccgccgaa tggggcggta ctggcagcac
    24181 cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga
    24241 atttttcccg ctcgggggaa accagaccgc aaaagagtcc gaagcgagtg ttgttcgcca
    24301 tccgaatcgc ctcatcaaaa gtatcataac gccagacgcg caaaagcggc ccgaacacct
    24361 cttcatccgg cacaccgcca acgcccgtca tttcaatgat ccctggtgtc agcaacgatg
    24421 tccctgcttg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct
    24481 gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct
    24541 gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc agactagcaa
    24601 gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt
    24661 gcccgactgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca
    24721 cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct
    24781 gaccggagag ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac
    24841 cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt
    24901 tcagcacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc
    24961 acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga
    25021 tatgtccgtt cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt
    25081 gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac
    25141 gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg
    25201 ccgcttccca gcgcggctta cccgtttctc tggcaataat cgcggttaat tcggctttat
    25261 tgctttccag caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc
    25321 gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc tgagcggcat
    25381 cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg
    25441 cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc
    25501 gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc
    25561 ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg
    25621 gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc
    25681 tgccacttcc accagccgac ttttacggat ggcgcgcacg cggtcgatgt cacactcaag
    25741 cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag
    25801 caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg
    25861 ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg
    25921 cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag
    25981 gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac
    26041 tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa
    26101 atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct
    26161 gccggtgtga tcgttactaa gaaacagcgt cggcaatgcg ttatagacat tcagctcttt
    26221 tgaggcgtga accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc
    26281 cacctcaata gcgcaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac
    26341 atagccctgc tcgcttttgg gcagttcacc ttgccaggtt ttgattgccc tttcgatacg
    26401 cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc
    26461 aagctgcatc agcgccgaga catctgagcg ctcaacggga cggatgacca tcatgatgaa
    26521 cctcggctaa caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct
    26581 tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc
    26641 atcacgcctg ctttcgccgc ttccagagag atctgtttcg cttgcccggc gtaatcggca
    26701 ttcagtacac agccaatcaa caaacctaag ccgcgaactt cactgaacaa accatagcgg
    26761 tgattaatag tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc
    26821 atctctggtg tgttgatgag ctccagcact ttgcctgcta ccgccgaggc cagcggatta
    26881 ccgccatagg tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc
    26941 aacaacgcac cgaccgggaa accgccgccc agcgctttgg cggtggttaa cagatcgggc
    27001 gtcacgccgt agtgcatata ggcatacagc tcaccagtgc gcccgacgcc ggtttgtacc
    27061 tcatcaaaaa tcagcaaggc attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac
    27121 gcgttgctgg ctggcaccac accgccttcg ccctagatgg gctcgacaat caccgcacag
    27181 gtggcgtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata
    27241 tccgccggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta
    27301 aacagcgtgc gaccatgaaa cgcatttttg aacgccacga tgccactctt atggctgccg
    27361 tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg
    27421 gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc
    27481 agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc
    27541 agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg
    27601 aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt
    27661 ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt
    27721 ggctgagaca tagcgacctc tacagtaaat aattcgttat ttgcatgtta acaataagta
    27781 atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa
    27841 acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca
    27901 tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg
    27961 cagaagtaaa atataattct agaattgtga tcattgacga aatttactgg aaattactgc
    28021 gccattctga cgcagcgcgc accaaaagcg ggcatttttt gcgccatcat tgacatcatt
    28081 aacaaccatc gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca
    28141 tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc
    28201 aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat
    28261 gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg
    28321 aagctcggct acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg
    28381 accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag
    28441 cggcggatta ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt
    28501 tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt
    28561 tatcagaatc tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt
    28621 atgggcgata tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt
    28681 aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac
    28741 aggctgatga gctggggggt ggtcgatacc ttccgccatg ctaatccgca aacagcagat
    28801 cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc
    28861 gacctgctgc tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat
    28921 gaaattcgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc
    28981 taatttagca gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa
    29041 caaactgtca gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta
    29101 gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga
    29161 gcgtaacttt ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt
    29221 cggtttattc gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg
    29281 attgttcggt tatagcctct atattctgtt attcatcaat gccccctctt tctgttacca
    29341 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg acacaactct
    29401 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    29461 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    29521 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    29581 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    29641 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    29701 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    29761 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    29821 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    29881 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    29941 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    30001 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    30061 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    30121 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    30181 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    30241 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    30301 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    30361 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    30421 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    30481 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    30541 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    30601 tcatcatcct gttattcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    30661 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    30721 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    30781 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    30841 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    30901 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    30961 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    31021 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    31081 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    31141 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    31201 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    31261 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    31321 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    31381 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    31441 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    31501 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    31561 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    31621 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    31681 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    31741 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    31801 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    31861 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    31921 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    31981 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    32041 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    32101 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aa
//
LOCUS       DAJTNW010000043        32130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNW010000043 DAJTNW010000000
VERSION     DAJTNW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO179_002065"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO179_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5532127.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO179_002066"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO179_002066"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5532128.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO179_002067"
     CDS             complement(3736..4092)
                     /locus_tag="OO179_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5532129.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO179_002068"
     CDS             complement(4096..4512)
                     /locus_tag="OO179_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5532130.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO179_002069"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO179_002069"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5532131.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO179_002070"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO179_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO179_002071"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO179_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5532132.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO179_002072"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO179_002072"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5532133.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO179_002073"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO179_002073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5532134.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO179_002074"
     CDS             10521..11504
                     /locus_tag="OO179_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5532135.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO179_002075"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO179_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5532136.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO179_002076"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO179_002076"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5532137.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO179_002077"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO179_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO179_002078"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO179_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5532138.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO179_002079"
     CDS             complement(15306..15515)
                     /locus_tag="OO179_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5532139.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO179_002080"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO179_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5532140.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO179_002081"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO179_002081"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5532141.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO179_002082"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO179_002082"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5532142.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO179_002083"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO179_002083"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5532143.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO179_002084"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO179_002084"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5532144.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO179_002085"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO179_002085"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5532145.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO179_002086"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO179_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO179_002087"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO179_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5532146.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO179_002088"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO179_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5532147.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO179_002089"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO179_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5532148.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO179_002090"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO179_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5532149.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWVHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO179_002091"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO179_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5532150.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO179_002092"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO179_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5532151.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8246 c         7782 g         7930 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggtgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt
//
LOCUS       DAJTNW010000044        31664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNW010000044 DAJTNW010000000
VERSION     DAJTNW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..1256)
                     /gene="yeaG"
                     /locus_tag="OO179_002093"
     CDS             complement(<1..1256)
                     /gene="yeaG"
                     /locus_tag="OO179_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5532152.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGL"
     gene            1692..2438
                     /gene="mipA"
                     /locus_tag="OO179_002094"
     CDS             1692..2438
                     /gene="mipA"
                     /locus_tag="OO179_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5532153.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2528..3289
                     /locus_tag="OO179_002095"
     CDS             2528..3289
                     /locus_tag="OO179_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5532154.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3340..4224)
                     /locus_tag="OO179_002096"
     CDS             complement(3340..4224)
                     /locus_tag="OO179_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5532155.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4308..5303)
                     /gene="gapA"
                     /locus_tag="OO179_002097"
     CDS             complement(4308..5303)
                     /gene="gapA"
                     /locus_tag="OO179_002097"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5532156.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5645..6058
                     /gene="msrB"
                     /locus_tag="OO179_002098"
     CDS             5645..6058
                     /gene="msrB"
                     /locus_tag="OO179_002098"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5532157.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6100..6372
                     /locus_tag="OO179_002099"
     CDS             6100..6372
                     /locus_tag="OO179_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5532158.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6742..7818
                     /locus_tag="OO179_002100"
     CDS             6742..7818
                     /locus_tag="OO179_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5532159.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7845..9224
                     /locus_tag="OO179_002101"
     CDS             7845..9224
                     /locus_tag="OO179_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532160.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9241..10286
                     /locus_tag="OO179_002102"
                     /pseudo
     CDS             9241..10286
                     /locus_tag="OO179_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10307..11143
                     /locus_tag="OO179_002103"
     CDS             10307..11143
                     /locus_tag="OO179_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5532161.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11148..12095
                     /locus_tag="OO179_002104"
     CDS             11148..12095
                     /locus_tag="OO179_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5532162.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12105..13085
                     /gene="ydjG"
                     /locus_tag="OO179_002105"
     CDS             12105..13085
                     /gene="ydjG"
                     /locus_tag="OO179_002105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5532163.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13222..13980
                     /locus_tag="OO179_002106"
     CDS             13222..13980
                     /locus_tag="OO179_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5532164.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14098..15456
                     /locus_tag="OO179_002107"
     CDS             14098..15456
                     /locus_tag="OO179_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532165.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15549..16190)
                     /gene="pncA"
                     /locus_tag="OO179_002108"
     CDS             complement(15549..16190)
                     /gene="pncA"
                     /locus_tag="OO179_002108"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5532166.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16201..17217)
                     /gene="ansA"
                     /locus_tag="OO179_002109"
     CDS             complement(16201..17217)
                     /gene="ansA"
                     /locus_tag="OO179_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5532167.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17384..19240)
                     /gene="sppA"
                     /locus_tag="OO179_002110"
     CDS             complement(17384..19240)
                     /gene="sppA"
                     /locus_tag="OO179_002110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5532168.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19401..19952
                     /locus_tag="OO179_002111"
     CDS             19401..19952
                     /locus_tag="OO179_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5532169.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20069..21112
                     /gene="selD"
                     /locus_tag="OO179_002112"
     CDS             20069..21112
                     /gene="selD"
                     /locus_tag="OO179_002112"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5532170.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21117..23078
                     /gene="topB"
                     /locus_tag="OO179_002113"
     CDS             21117..23078
                     /gene="topB"
                     /locus_tag="OO179_002113"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5532171.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23205..24245
                     /locus_tag="OO179_002114"
     CDS             23205..24245
                     /locus_tag="OO179_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5532172.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24362..25705)
                     /gene="gdhA"
                     /locus_tag="OO179_002115"
     CDS             complement(24362..25705)
                     /gene="gdhA"
                     /locus_tag="OO179_002115"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5532173.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            25941..26213
                     /locus_tag="OO179_002116"
     CDS             25941..26213
                     /locus_tag="OO179_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5532174.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26179..26586)
                     /gene="nudG"
                     /locus_tag="OO179_002117"
     CDS             complement(26179..26586)
                     /gene="nudG"
                     /locus_tag="OO179_002117"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5532175.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26673..27293
                     /locus_tag="OO179_002118"
     CDS             26673..27293
                     /locus_tag="OO179_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5532176.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27302..28609)
                     /gene="ynjE"
                     /locus_tag="OO179_002119"
                     /pseudo
     CDS             complement(27302..28609)
                     /gene="ynjE"
                     /locus_tag="OO179_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28676..29329)
                     /locus_tag="OO179_002120"
     CDS             complement(28676..29329)
                     /locus_tag="OO179_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5532177.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29329..30846)
                     /locus_tag="OO179_002121"
                     /pseudo
     CDS             complement(29329..30846)
                     /locus_tag="OO179_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30819..31664)
                     /locus_tag="OO179_002122"
                     /pseudo
     CDS             complement(30819..31664)
                     /locus_tag="OO179_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7930 a         7579 c         8211 g         7944 t
ORIGIN      
        1 agaccgttca tcccttcatc gacaccggcg tagtcacgat attcctgata cgacttggct
       61 ttcggatcgg tgtctttcag actttcgcca tcataaaccc gcatctttga ataaatgctg
      121 gagttttctg gctctttcag gcgcgaaaga atggaaaaac gtgacagtgt ttcgagcgtg
      181 ccaggggcgc atggggcgtg agtcaattca ctgtgattaa gcaatttctc gtagattttg
      241 atctcttcgg aaatgcgcaa gcaatacggc accttcacga tgtaaacacg gtcgaggaag
      301 gcttcgttgt ttttgttatt acggaaagtg acccattcgg acttgttcga gtgggcgaga
      361 ataatcccgt tgaacggcag ggcggagata ccttccgtcc cgttgtagtt accttcctga
      421 gtggcggtta acaagggatg cagcactttt atcggtgctt taaacatctc aacgaattcc
      481 atgatcccct gattggcgcg gcacagcgca ccggaatagc cgtaggcgtc cgggtcattc
      541 tgcgcgtagt gttcgagttt acgaatatcg actttcccaa ccagcgcgga gatgtcctgg
      601 ttgttctcat caccgggttc cgttttggcg atagcaattt gttgcagaat tgacggccag
      661 accttcacta cccggaactt agtgatatcg ccaccaaatt catgcaggcg ttttgccgcc
      721 catggcgaca tgatggtgcc gagataacgg cgaggaatgc catactcttt ttccagaatc
      781 tgagcatctt cctgcggatt gaaaagacag aacggatgat cgttgaccgg gctacgctca
      841 ccgttggcgc tcaatacata aatcggtacg agctgcatta atgatttcag tcgctcagca
      901 agcgatgatt tacccccacc cacaggcccc agcagataca ggatttgttt cttctcttcc
      961 agcccctgag ccgcgtgttt cagataagag acaatctgtt caatcgcgtc ttccatgccg
     1021 taaaactctt caaatgccgg ataacgtgca atgacccggt tagaaaagag tcgagaaagt
     1081 ctggggtcct gggctgtatc gaccatgaca ggctcaccga tagccatcaa tagccgctca
     1141 gccgcgttgg cataagcact gcgatcttgc cgacaagtgg taagaaactc ctgcagtgtg
     1201 aactcttcgt ccttggcagc ttcatatcgc tggcgatagt gatcaaatat attcatggta
     1261 tgccgtcctt tcgtttttta gcacaggtta agagccgttc atatgaatag tagaggctcc
     1321 cggaagaggt aaactgaacg acgtctcttg ctaaaacaca gcaactctca tgccatttta
     1381 tcgacgatga gaacaacgtg cccggtgata caccttcaaa attaagcgta gatggcaaat
     1441 ggaaaacttg cctgcacggc tttcttaatt tcaatggcat atcaacaact catccataaa
     1501 ttttgcataa ttaatgtaaa gaccaggctc gccagtaacg ctaaatccat ttggctgtaa
     1561 gcgcggtgtc atccgcggca ggaaaattaa acagttactt taaaaaatga aaacgtaaaa
     1621 aggttgggtt tcgatgtatt gacgggtaaa ctttgtcgcc cgctaaacat ttgtttaagg
     1681 aatgattaat tgtgaccaaa ctcaaacttc tggcacttgg agtgcttatc gcaacgtctg
     1741 caggcgtagc gcacgctgaa ggtaaatttt ccctgggcgc aggcgtaggt gtcgttgagc
     1801 acccatataa agattacgat accgatgttt acccagtacc ggtaatcaac tatgaaggcg
     1861 ataacttctg gttccgtggc ttaggtggtg gttactacct gtggaatgac gcaacggata
     1921 aactttcaat taccgcttac tggtcgccgc tttacttcaa agcgaaagac agtggcgatc
     1981 accaaatgcg tcacctggat gaccgtaaga gcaccatgat ggctggtctg tcttatgctc
     2041 actttaccca gtacggttac ctgcgtacca ccctggctgg cgataccctg gataacagca
     2101 acggcattgt ctgggatatg gcctggttgt atcgttacac caacggtggc ctgaccgtga
     2161 ctccgggtat tggtgtgcag tggaacagcg aaaaccagaa cgaatactat tatggcgtat
     2221 cgcgcaaaga gtccgctcgc agcggtctgc gtggctataa cccgaatgac agctggagcc
     2281 cttaccttga gctgagcgcc agctacaact tcctcggcga ctggagtgtt tacggtaccg
     2341 cgcgctacac ccgtctgtct gatgaagtta ctgacagccc gatggtggat aaatcctgga
     2401 ctggcctgat ttctaccggg atcacctaca aattctgata atgcatcttt ccagggcatt
     2461 ttgtgcataa aaacagggcg tcacgccctg ttttgcatta cggtggtgca atacctggga
     2521 ggaaacaatg caacaaaaaa tgattcaatt tagtggcgat gtctcactgc cagccatagg
     2581 gcagggaaca tggtatatgg gcgaagatgc cagtcagcgc aaaacagaag ttgctgcact
     2641 acgcgcaggc attgaactcg gtttaaccct cattgatacc gccgaaatgt atgccgatgg
     2701 cggtgccgaa aaggtggttg gggaagcatt aaccggtctg cgagagaagg tctttctcgt
     2761 ctctaaagtc tatccgtgga atgctggcgg gcaaaaagcg ataaatgcat gcgaagccag
     2821 tttacgccgt ctcaatactg actatctcga tctctactta ttacactggt ctggcagttt
     2881 cgcttttgaa gagactgttg ccgcgatgga aaaattgatc gcccagggaa aaatccgtcg
     2941 ctggggcgtt tctaaccttg gttatgccga tatgcaggaa ctctggcagc tgccgggggg
     3001 aaatcagtgt gccactaatc aggtgcttta ccatctcggt tcacgaggga ttgagtacga
     3061 tctactcccc tggtgccagc aacagcagat gccggtgatg gcttacagtc cattagccca
     3121 ggccgggcgg ttgcgcaatg gactgttaaa aaacgcggta gtcaacgaaa ttgcacatgt
     3181 ccaacaacat gcagctgcgc ttgaggtcga actttcgtca gcggaattag ctatgctgga
     3241 taaggcatat ccggcaccaa aaggaaaaac tgcgctggat atggtgtgat gttgatacgt
     3301 gccggatgca tcgcacccgg cacacaacat taacgcaaat taacgtttgg caacgcgaat
     3361 ggattgcgcc agatgtgcag gtttctcttt ggtgactttt tgcgtttctg aagcgtaagc
     3421 cgtttctaca caaacaaagg tcttgtagcc atcgtccggc atgtcgccca tgctaactga
     3481 aagcgccggt cccgggttcc agccgacaac gttcagatga tgctggtggc ctacggcgat
     3541 aatacgattc agcgcttcat cattaatcac gctgcaatct tgcggattca gatacacgcg
     3601 atcggtacgg tcagggaagg tctgaatacc gtcggtcagt acatcttctt tcgcgtcatt
     3661 cactttatca atgaagcgat cgcccagccc actgacgctt accttagcga tatcacccac
     3721 gttaaagtag gtatgcaggg cagaggtggt ttcaaattcg ccatgtgatt caagatcgat
     3781 ttcacaggtt ttacccacgc ggaaatgcgc taacagcgta aagtcgtgcg gccagaattt
     3841 tttcgtctct tcgctttgtg tcaattcaaa agtcagcgct acgccatcag catcttcatg
     3901 atgtgatttc agcgtccacg gcaggttgcg ggcaaaaccg tgcgcaggca gaccttgttg
     3961 tgccgccgga ccaaaccacg gccagcaaac cggtacgcca ccgcgaatag cgacgccatt
     4021 tttgaacggt gtgttgttgc tcaaccacag aacttcttct tcacccgcag gtttccacga
     4081 gagaaggtgt gcgccctgta atgcaaaaga ggcttttacc tggggatgat cgaccacaat
     4141 gaggtccagt tcatccagtt tacgacggga gaggacaggg gagatttgtt cgatgaccgg
     4201 aagggcaaaa attttcttaa tcatgacgca gtcctttaac ttcattttat caggtaaaaa
     4261 aaagagcgac cgaagtcgct ctttttagat cacagtgtca tctcaactta tttggagatg
     4321 tgagcgatca ggtccagaac tttgttggag taaccggttt cgttgtcgta ccaggatacc
     4381 agtttcacga agttgtcgtt cagagcgata ccagctttag catcgaacac ggaagtgcaa
     4441 acttcgccgt tgaaatcggt agatactacg tcatcttcgg tgtagcccag aacgcctttc
     4501 atttcgcctt cagcagcagc tttaacggca gctttgatct gctcgtaagt tgcagctttt
     4561 tccagacgaa cggtcaggtc aactacggat acgttcgggg tcggaacgcg gaacgccata
     4621 ccagtcagtt tgccattcag ttctggcagt actttaccta cagctttagc agcaccggta
     4681 gaggacggga tgatgttctg ggaagcgccg cggccgccgc gccagtcttt gtgagacggg
     4741 ccatcaacgg ttttctgagt agcggtagta gcgtgaacgg tggtcatcag accttcgatg
     4801 atgccgaagt tatcgttgat aactttagcc agcggagcta ggcagttggt ggtgcaggaa
     4861 gcgttggaaa cgatgtcctg gccagcatat ttgtcgaagt tagcgccttt aacgaacatc
     4921 ggagtgttgt ctttagacgg accagtcata accactttct tcgcaccagc ggtgatgtgt
     4981 ttacgagcag tttcgtcagt caggaacaga ccagttgctt cagcgacaac gtcaacaccc
     5041 acttcgtccc atttcaggtt agccggatca cgttcagcgg taacacggat ttttttaccg
     5101 ttaacgatca gatgaccgtc tttcacttca acggtaccgt cgaaacggcc gtgagtggag
     5161 tcatatttca gcatgtatgc catgtaatca gcgtctaaca ggtcgttgat tgcaacgatc
     5221 tcgatgtcag aacgtttctg agcagcacgg aaaacaatgc gaccgatacg gccaaaaccg
     5281 ttgataccta ctttgatagt catatattcc accagctatt tgttagtgaa taaaaggttg
     5341 cctgtaaaat tacaaaaacc ttacgcagcg tcaagcggaa tcgtgtcaat cattgcgaca
     5401 aatcaatcct gtgcctaagc attacgcgac tgactcgcct cacttttcct ttgggcttga
     5461 gaccacatgg ggtcggcgcc ccgaatttta aagggcaatt acgataaaaa tgtgattttc
     5521 atcacgattt aggtgcagcc atttcgtcga gatcaagttt aggacaaaat tttgccagtt
     5581 ggtgttaatg ttttgttaga atcagtcacg taatgtgagc acgattaaag tgagatgtga
     5641 gtaaatggcg aataaacctt cggcagaaga actgaaaaaa aatttgtccg agatgcagtt
     5701 ttacgtgacg cagaatcatg ggacagaacc gccatttacg ggtcgtttac tgcataacaa
     5761 gcgtgacggc gtatatcact gtttgatctg cgatgccccg ctgtttcatt ccgaaaccaa
     5821 gtatgattcc ggctgtggct ggcccagttt ctacgaaccg gtgagtgaag aatccattcg
     5881 ttatatcaaa gacttgtcac atggaatgca gcgcatagaa attcgttgcg gtaactgtga
     5941 tgcccatctg gggcatgtct ttcccgacgg gccgcaacca acaggcgaac gttattgtgt
     6001 taactctgct tctttacgct tcactgatgg cgaaaacggc gaagaaatca acggttgaaa
     6061 aaacgattca gcaacttatt ccacaggagc ggaaaaatta tgaatcttga tgacattatc
     6121 aacagcatga cgcctgaggt ataccagcgt ttgtcgaccg ccgttgaact ggggaaatgg
     6181 cctgatggcg ttgcgttgac cgaggaacaa aaagagaatt gcctgcaact ggtgatgctg
     6241 tggcaagccc gccataatac cgaagcacag catatgacaa ttgacacaaa tggtcagatg
     6301 gtcatgaaga gcaaacagca gttaaaagaa gattttggta tctcagcaaa gcctattgcc
     6361 atgtttaagt aagtggttca tccccttttt gccgcggcta tgaaggggat agccaacaat
     6421 gaccccacga ctcgtctcaa ttatctcgac ccgacctggc taagtcccag tccgatctct
     6481 tgcagtcgcg ttcacgcctg aatgacttct cctgaactta agtctcagac ctatttggcc
     6541 ggtaatccct ctcgaatttg ctctttttgc gtcccgcctt ccaccaacct gtaacagacc
     6601 cagccatttt ctgcgtaatt gtgatctgag ttgtaaaatg caggtcatta tctgtgttgt
     6661 gcagatttaa tattgacctc aaaaatcatg tgggtaagta tgatggccat ctaagcaaaa
     6721 caaaacacag aggatgacgc aatgaaagca ctggctcggt ttggcaaggc ctttggcggc
     6781 tacaagatga ttcatgtccc acaacccatt tgtggcccgg aagacgtagt gattgaaatt
     6841 aaagccgcgg caatctgcgg cgcagacatg aagcactaca atgtcgatag cggttctgat
     6901 gagtttaact ctatccgcgg ccatgagttc gcaggttgta ttgcgcaggt tggtgaaaaa
     6961 gtcaaagact ggaaagtggg gcaacgcgtc gtgtcggata acagcggtca cgtttgcggc
     7021 gtttgtccgg cctgtgaaca gggtgatttt ctgtgttgta cagaaaaagt aaaccttggt
     7081 ctggataaca atacctgggg cggtggtttt tccaaatatt gtctggttcc tggtgaaatt
     7141 ctcaaaattc atcgtcatgc gttgtgggaa atccctgatg gtgttgatta tgaggacgca
     7201 gccgtacttg accctatctg taatgcctat aaatccatcg cgcagcaatc gaaattcctt
     7261 cctggtcagg atgttgtcgt cattggcact ggcccactcg ggctgttttc cgtacaaatg
     7321 gcgcggatta tgggggcggt aaatatcgtc gtcgttggtc tgcaagaaga tgtggcggtc
     7381 cgcttcccgg ttgcaaaaga actgggggcg acggcagtag taaatggttc taccgaagat
     7441 atggtggcgc gctgccagca aatttgtggc aaagataatc tgggactggt gattgaatgc
     7501 tccggtgcca atatcgcact gaaacaagcc atcgaaatgc ttcgcccgaa tggggaagtg
     7561 gtacgcgttg gaatgggctt caaacctctt gatttctcga ttaatgacat taccgcctgg
     7621 aataaaagca tcattgggca tatggcctat gactccacct catggcgtaa cgctatccgg
     7681 ctattagcca gcggcgctat caaagtcaaa ccgatgatca cgcatcgtat cggcctgtcg
     7741 caatggcgcg aagggtttga tgcgatggtc gataaaaccg caatcaaagt gatcatgact
     7801 tacgactttg atgaataact actctcctga aggaacgtta tcgcatggaa cagataacaa
     7861 aaccgcattg tggtgcacgg ctggatcgct taccggattg ccgctggcat tcgtcaatgt
     7921 ttgccatcgt cgcgtttggc ttgcttgtct gctggagtaa tgccgttggt ggcttgatcc
     7981 tcgcgcagct gaaagcgttg ggctggacag ataattccac tacagccaca ttctcagcaa
     8041 tcacgaccgc cggaatgttt ctcggtgcgc tggtgggcgg catcattggc gacaaaaccg
     8101 gtcgcagaaa tgcgttcatc ctctatgagg ccattcatat tgcctcgatg gttgtcggtg
     8161 ctttctcacc gaatatggat tttcttattg cctgccgttt tgtgatggac gttgggctgg
     8221 gagctttact ggttacactg tttgctggtt tcaccgaata tatgcccggt agaaatcgcg
     8281 gaacgtggtc cagtcgggtt tcctttattg gcaactggtc atatccgctc tgctcactga
     8341 ttgcgatggg actcacgccg ctgattagtg cagagtggaa ctggcgggta caactgctta
     8401 ttcctgcaat attgtcgctt atcgctacgg cgctggcctg gcgctacttt cctgaatccc
     8461 cgcgctggct ggaatcgcgc ggacggtatc aggaagccga gaaagtcatg cggagtatag
     8521 aagaaggcgt catacgccag acgggtaaac ctttgccgcc tgtggttatt gctgatgacg
     8581 gtaaagcgcc acaagcggtg ccgtattcag ccttactgac aggagtatta ctgaaacgcg
     8641 tgatattagg ctcttgtgtg ctgattacca tgaacgttgt gcagtacaca ctaattaact
     8701 ggttgccaac aatattcatg acccagggga ttaatttaaa agactcgatt gttttaaata
     8761 ccatgagtat gtttggcgcg ccatttggta tttttattgc catgctggtg atggataaaa
     8821 ttccgcgtaa aacaatgggt gtggggctat taatcctgat tgcggtgctc ggatatatct
     8881 attcactgca aaccagtatg ttgctcataa cgctgattgg tttcttcctg attactttcg
     8941 tctatatgta cgtttgctat gcctcggcag tgtatgtccc tgaaatctgg ccgacagagg
     9001 ccaaactccg tggctccggt ctggcgaatg cggtagggcg aatcagtggt attgccgcac
     9061 cttatgccgt tgcagtgctg ctcagtagtt atggtgtaac gggagtcttt attcttctgg
     9121 gggctgtttc aaatattgtc gcaattgcta tcgccaccat tggaattgaa accaaaggtg
     9181 tctccgttga aagtttaagt attgatgcag tagccaataa ataatacggg tggattcaaa
     9241 atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa tgaaaattat ttcagcagaa
     9301 ataccagtgc ctaaagaaga tgaagttttg attaaagtag aatatgtcgg tatttgtggt
     9361 tcagatgtac atggttttga atcaggcccg tttattccgc ctaaagaccc aaatcaagaa
     9421 attggcctgg gtcatgaatg cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa
     9481 tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac
     9541 tgtctggaag gcaaatataa catctgcccg gacgttgatt ttatggcgac acaacccaac
     9601 taccgcggcg cattaacgca ctatctgtgt catccggaga gctttactta caaactgccc
     9661 gacaatatgg acacgatgga aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc
     9721 gcgatgctgg cagatgttaa accgggtaag aagataatta ttctgggagc aggttgtatt
     9781 ggtttgatga cgttgcaagc gtgcaaatgc ctgggcgcaa cggaaattgc cgtcgttgat
     9841 gtactggaaa aacgtctggc aatgggggaa cagcctggtg cgacagtggt tattaatggc
     9901 gcaaaagagg acactattgc acgctgtcag caatttaccg aagacatggg cgcagatatt
     9961 gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg cacctttaat ctggtaatgc
    10021 gcggcggtaa aattatgatt gttggtactg tacccggcga ttcggcaatc aatttcctca
    10081 aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt tatccggtca
    10141 cgattgaagc tatttcttca gggcgattcg atgtgaaatc gatggtgacg catatttacg
    10201 attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc gacattatta
    10261 aaggcgttat taaaattagc gattaattaa tacgggagaa cagaacatgc tcgcagatat
    10321 cagatattgg gaaaacgatg ccacaaataa atattatgca attgcccatt ttaacgtatg
    10381 gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa gaagcgaaat ccccggttat
    10441 tatttctttt agtacaggtt ttgtcggtaa cacctcattt gaagatttct ctcacatgat
    10501 ggtatcaatg gcacaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
    10561 tattgagatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
    10621 tgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tctttcatcc
    10681 gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg tttatgagga
    10741 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
    10801 gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata cgtcagagcc
    10861 gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc cgctggtttt
    10921 gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt tgggtattgc
    10981 gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtagcagtga aagaaaacca
    11041 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
    11101 ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
    11161 acgttatttg tataggtgcc gctattgttg atattccatt acaaccggtc agtaaaaata
    11221 tctttgatgt agattcttat cctcttgaaa gaatcgcaat gagcaccggg ggcgatgcaa
    11281 taaatgaagc aacaattatt tctcgtctgg ggcatcgtac cgcattaatg agccgcattg
    11341 gtaaagatgc ctcagggcaa tttattctcg accattgtcg caaagagaat attgatattc
    11401 aaagcctgaa acaggacgtc agcatagata catctattaa cgttggactg gtgacggagg
    11461 atggcgagcg gacgtttgtc acgaaccgta acggcagttt gtggaaatta aatattgacg
    11521 atgttgattt tgcgcggttt cctcaggcga aattattatc actggccagt attttcaaca
    11581 gtcctctatt ggatggtaaa gcgctaacag aaatttttac gcaagcgaaa gcccggcaga
    11641 tgatcatttg tgccgatatg atcaaaccgc ggttgaatga aacgctggat gatatttgcg
    11701 aagcattgag ttatgtcgat tatctgtttc caaattttgc cgaggaaaaa ttactcacag
    11761 ggaaagagac actggatgaa attgctgact gctttcttgc gtgcggcgta aaaacggtgg
    11821 tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg atgaaggtgc
    11881 cggcggtcgc aggaataacc gccatcgaca ccattggcgc gggcgataac tttgcttcag
    11941 gttttattgc ggcactgtta gaaggcaaaa atctgcgtga atgcgcacgc tttgccaatg
    12001 caacggcggc tatctcggtt ctaagcgtcg gtgccaccac cggcgtaaaa aacagaaagc
    12061 tggtcgaaca attgctggaa gaatacgaag gataatgaag gcaaatgaaa aagatacctt
    12121 taggcacgac ggatataacg ctttcgcgaa tggggttggg gacatgggcc attggcggcg
    12181 gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat tgatacgatt cttgaagccc
    12241 atcgttgtgg cattaatctg attgatactg cgctaggata taactttggc aatagtgaag
    12301 ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta gaaaccaaat
    12361 gcggcattgt ctgggaacga aaaggaagtt tattcaacaa agttggcgat cggcagttgt
    12421 ataaaaacct ttccccggaa tctatccgcg aagaggtaga agccagcttg caacgtctgg
    12481 gtattgatta catcgatatc tatatgacgc actggcagtc ggtgccgcca ttttttacgc
    12541 cgatagctga aactgtcgca gtgcttaatg agttaaaggc tgaagggaaa attcgcgcta
    12601 taggcgctgc taacgtcgat gctgaccata tccgcgagta tctgcaatat ggtgaactgg
    12661 atattattca ggcgaaatac agtattctcg accgggcaat ggaaaacgaa ctgctgccgc
    12721 tatgtcgtga taatggcatt gtggttcagg tttattcccc gctagagcag ggattgttga
    12781 ccggcaccat cactcgtgat tacgttccgg gcggcgctcg ggcaaataaa gtctggttcc
    12841 agcgtgaaaa catgctgaaa gtgattgata tgcttgaaca gtggcagcca ctttgtgctc
    12901 gttatcagtg cacaattccc actctggcac tggcgtggat attaaaacag agtgatttaa
    12961 tctccattct tagtggggct actgcaccgg aacaggtacg cgaaaatgtc gcggcactga
    13021 atatcaactt atcggatgca gacgcaacat tgatgaggga aatggcagag gccctggagc
    13081 gttaaatact caacacgatt cacctctgtt tctgtgaaaa ctgtgatgaa aatctgtgat
    13141 tcaaataggt tattttgaaa gtaaacatcg gttatgcgat aatcgcgcta atgtgatgta
    13201 aaaagataca ggggtgtaat cgtggcggca aaagacagga ttcaggcaat taagcaaatg
    13261 gttgccaacg ataaaaaggt gacagtctca aatttgagtg ggatttttca ggtaaccgaa
    13321 gaaaccattc gccgcgatct tgagaagctg gaagatgaag gctttttgac cagaacctat
    13381 ggtggtgctg ttttaaatac agcggtgttg acggagaata tccattttta taagcgcgct
    13441 tcatcgtttt atgaagagaa gctgcttatt gcacgcaagg cactaccctt tatcgacaat
    13501 aaaaccacca tggcagccga ttccagtagt accgtaatgg aattgctcaa attgttacag
    13561 gaccgtagtg acctgacgtt gctaaccaac tcggcggaag cgattcatgt tctggctcag
    13621 tcagaaatta aagtcgtttc aacgggtggg gaactaaaca aaaatacact ttccctgcaa
    13681 ggaagaatta ctaaagagat catcagccgc taccatgtcg atatcatggt aatgagttgt
    13741 aaaggtcttg atattaacag cggcgcgctg gactctaatg aagcggaagc tgaaatcaaa
    13801 aagacaatga tccgtcaggc gacagaagtt gcgttattgg ttgatcactc taagtttgat
    13861 cgtaaagctt ttgtccagtt agctgacttt agtcatatta attatataat aactgataaa
    13921 tcaccgggtg cagagtggat agcattttgc aaagacaata atatccagct ggtgtggtaa
    13981 tttaagcaca cgttgtcaat attatttgtt cctgcgactg gaaataaaat gatgataaag
    14041 atgcggcaag tgaatttgcc gcaattgcta ccttttatag caaatccgag gcacccaatg
    14101 gaacaatatg atcaaattgg cgcaagactg gaccgcttgc ctttggcccg gtttcattat
    14161 cgtatatttg gtataataag ctttagtctg ttattaacgg ggtttttgag ttactcaggt
    14221 aatgtcgtct tagcaaagct ggtaagcaat ggatggtcaa ataatttcct ctatgccgcc
    14281 tttacctcgg cattaatgtt tggttatttc atcggctcac ttactggtgg gtttattggt
    14341 gactactttg ggcggcgcag ggcgtttcgc ataaatcttc tcatcgtcgg tattgctgca
    14401 acaggggccg cttttgtccc tgatatgtac tggctcattt tctttcgctt cctgatggga
    14461 acaggaatgg gggcgctgat tatggttggc tatgcctcat ttacggagtt tatccccgcg
    14521 acggtgcgtg gaaaatggtc cgcgcggctc tcatttgttg gtaactggtc gcccatgctg
    14581 tctgcggcga taggcgtggt ggttatcgct ttttttagtt ggcgaataat gtttcttctg
    14641 ggtggtattg gcatactgtt agcctggttt ctctcaggta aatactttat cgagtcgcca
    14701 cgatggctgg aagggaaagg gcaaatcgcc ggtgcagaaa gccaacttcg tgaagtagag
    14761 cagcaaattg aaagggagaa gagtattcgt ttaccccagc ttactttgaa ccagagcaac
    14821 agcaaggtta aggtaatcaa gggtaccttc aggctcctgt ttaaagggga aatgttacga
    14881 cgtacattag ttgcgataac tgttttaatt gcaatgaaca tttcgcttta taccattacc
    14941 gtatggatac cgaccatatt tgttaactcc ggcattgatg tcgataaatc aatattaatg
    15001 accgctgtta ttatgattgg tgctccggta ggaatattta ttgcagcatt aattattgat
    15061 cattttcctc gtcgattatt tggctccgcc ttacttatta ttattgccgt gttaggctat
    15121 atctattcaa gtcagactac agagtgggcg attttaatct atggtctggt gatgatcttc
    15181 tttttataca tgtatgtttg cttcgcgtcg gcggtttata tcccggagct ttggccaacg
    15241 catttacgcc tgcgcggttc gggtttcgtt aatgccgtcg gacggatcgt cgcagttttc
    15301 acgccctatg gcgttgcggc attattaaca cattatgggt cgatcacggt gtttatggtg
    15361 cttggtgtca tgttagtgct ctgtgcgctg gttctctcca tttttggcat cgaaacgcgg
    15421 aaggtgtcgc tggaagagat ttctgaggtg aattaaatct ttcgttgatg gcatcaattg
    15481 ccagatgtac cactgacgtg ttttatttgg cctacaatgc cagtacagaa ttgtaggcca
    15541 gcgtaaaatt acccctgcgt ctcttcccag tctgccagcg tatatagcgt tgccccagct
    15601 gctgacatct ccataaacgc gtgtgcactg tcctggggct ggatattcac gccacgacag
    15661 ccatcggtaa tcacgtttac cttataacct aactgtaacg cgtccagcac ggtaaacttc
    15721 acgcaatagt cagtagccag gcccataacg atcaattcat cgatttcatg atcgcgcaac
    15781 cagtcatcaa gcgcggtttt ctgccgacgt ccgttatcaa aaaaggcact gtaactgtca
    15841 actaaaggat tttcgccttt atggaacacc gctgcgatct ctttttgatt cagtaacgga
    15901 tgtaattgtg cgccttcact gttctgcaca cagtgttcag gccagaaagt ttgccgcagg
    15961 ccgccgagtt ggcctggcgt ataaggctct acgccgtgct ggctggcaaa gctgccgtga
    16021 ttcgccggat gccagtcctg gctggcgata accgcttctc cgcgcgactg gcaccagtca
    16081 ataaggcggt tagcaacctc caccgtactg tcaccttccg gtacggcgag cgcgccacca
    16141 gcacagaaat cattttgtaa atcgaccagt aacagggcgc gatggggcat tgcagtctcc
    16201 ttaatcatcc ggcgtcagtt cgccgcgcag gttttggctc atggccttgc gaatggtttc
    16261 agtatccagt tcctggctca gcaggtaatg cagtttggtt agcgtggctt ctacagtcat
    16321 atctgcaccg tcaataacgc cggcatgggc gagggcgtta ccggtggcat aaccactcat
    16381 attcacttta ccggacatac attgtgtcag gttgaccacc acaataccgc gatcgctggc
    16441 ttcttgtaat tcctgcagga aggctttgtt ttgtggcgca ttacccacgc cataggagcg
    16501 cagaatcaat gctttcaccg gttggcgcag aaaattgcgc acgacgtcag cagaaatccc
    16561 tggataaatc gtcaccacgc caattggttg tggcgtgatt ggatgaacga ttaattcccc
    16621 ttcaccgtgc ggggcgggtg gcgtattcaa acgacgaata tggataccag cttccagtaa
    16681 cggaggaagg tttggagagg caaacgcatc aaaaccatcg gcatgggctt tggtagtgcg
    16741 gttgccgcga tacaatcggt tattgaaaaa gagcgttact tcgttgatcg gataattcgc
    16801 cgcaacgtac aacgcattca gcagattaat ttgtccgtca gagcgtaact cagccagcgg
    16861 gatttgtgac cctgtcacaa tgaccggttt accgagattc tcgagcatga acgacagcgc
    16921 agaggcggta tacgccatcg tgtcggtacc gtgtagaatg acaaaaccat catagtcgtc
    16981 atagtgcgct ttaatatctt cagcaatatg ctgccagtct tccggcgtca tatcagaaga
    17041 atccatcaac ggcgtatatt catgaatggt gaaatctggc atctccgggc gatggaattc
    17101 cggcatcagc gccagttggc gttgtagatg acctgacacc ggaatataac cctgctcgga
    17161 acgctgcatc ccgatggtcc cgcccgtgta ggcgacgtaa attgatttct tttgcatgat
    17221 attgatatga atgatatcga aagagcaaaa gtatatacgt gaggcaggag gagcgcacct
    17281 gatactatgc ggataaggaa atatggggcg agacagcggg aagaggattc gtcgaggtaa
    17341 aaaataatgt ggccgacgat ctgtcggcca ctcagtacaa gacttaacgc acgttggcgc
    17401 aggtcaggca aaacgcataa cggttttgtg ggtcgttaaa cgcggccagc ttgtcgcttt
    17461 cacttttaac ggtagaggct accgaggcaa gcggtgcagg taacatggcc tggaacgcat
    17521 ctggcaacat tgcccggaca gaaccagaca tgttgtccat cactttgtcg aagaaggtcg
    17581 gttcatcaac gtagtattcc agatgccact gtttcacttt tgcgagctct gctgctttgg
    17641 caaccgcatc atcgaaatcc ccgagactat cgaccagccc gttagctttt gcatcctgac
    17701 cggtccagac gtggccctgg gcgattttat caatttgctc cggcgtcgaa tgacgcgcat
    17761 cagcaaccag cgtgataaag cgtttatagc cattctcaat gcttaattgc atcatctgct
    17821 gcgcttccgg cggcagtgcc ctggtgatag aaacatccgc cagcggtgaa gttgagacac
    17881 catcagtatg aacaccaatc gaatccagac tattttctac ggtggtgatc acgccgaaga
    17941 taccgataga accggtcagg gtgctggggt tagccacaat gtaattagct ggcgtggaaa
    18001 tccagtaacc accagatgcc gccatgccgc ccatcgatac aaccacagac ttacccgctg
    18061 cccgggctgc tgccagttca gcgcgaatca cttcagacgc ggtaacgctg ccgcctgggc
    18121 tattaacacg caggacaatc gctttcactt tcgggtcaag gcgagcgtcg cggatttgtg
    18181 ccgcagtggt atcaccgcca acattcccct gagtttcctc gccatccata attgcgccat
    18241 tagcgaagac gacaccgatg ctgtcaccgg tatctgccgg cgttttcaat gcgtaatcgt
    18301 aataactgat ggcgcgataa tttttatcag tcttactcca gccgaactct ttggtcagag
    18361 ctttttcgat ttcggcactc gatgccagtg catcgaccag cttgttttcc agtgcatatt
    18421 tcgcggtatc gccaccggtt ttggttaaac cctcaagcaa cccttgcgcg ccagggaata
    18481 cctgctgagc agggatctgc cggttagcgg caacagtatt cagatagttt tgccacagct
    18541 caccaatcca gcggctgtca gcttcgcggg ctgccggtga catatcatca cgaataaacg
    18601 gttcaacggc agatttatac gtacccacgc ggaacacatg ggtggaaact ttcagcttat
    18661 ccagcaacga tttgtagtac agaccgttgg tagcaaaacc gtgcagatca accacgcctt
    18721 gcggtgacag ccaaatttta ttggcgaaac tggcgagata atattgcccc tggctgtagt
    18781 tctcgccaac ggcataaacc ggtttcccgc tgtcacgaaa ctctttcagg gctttgccga
    18841 tgtactgcat agacggctgg tcgccgcctg cgaagttttt cagatccatc acgataccgg
    18901 tgatattgcg gtcgtccttc gcctggcgaa tggtgttgac gatatcaaac agtgagtttt
    18961 cctgcagacg atcggaactg gcaccaagca gctggcggct taatttacta aaccgctgag
    19021 aactgtctgg tttatcgacg atcacaccag aaatgtccag cagcagtgcg ccacgactgg
    19081 ccgtttcttt cgaatcacca ccactgacct gcatccagat ccccacacca accagtacga
    19141 ggaaaataaa gaacaggtta agtaccattt cacggacgaa attcagcaga cgccacgtcc
    19201 atttaaaaaa tccggcaata aatcgccaaa gggttcgcat gtattctccc aacttaaggt
    19261 cacacctgtc acaggcgcaa tagatgtggt tatcgtaatg acccaaccgg cacttgtcag
    19321 caggaattgc ggactgtgct gtaacaaaat ccactctcgt gttaattttg tgaataaata
    19381 tcacgacagg agttaatcaa atggatgcac tcgaactatt gatcaatcgc cgtagcgcct
    19441 cccgcctggc tgaacccgcg ccaacgggtg aacaactgca aaacatcctg cgtgcgggta
    19501 tgcgtgcgcc ggaccataag tccatgcaac cgtggcattt ttttgtgatt gaaggggaag
    19561 ggtgcgagcg tttcagcgcc gtactggaac agggggcgat tgctgccggt agtgatgata
    19621 aagctatcga caaagcccgt aatgcgccgt tccgcgcacc gctcatcatc acggtggtgg
    19681 cgaaatgcaa agaaaatcat aaagtcccgc gatgggaaca ggaaatgtct gccggatgcg
    19741 cggtcatggc gatgcaaatg gcagccgttg cccagggatt tggcggcatc tggcgcagtg
    19801 gcgcattaac tgaaagtccg gtagtgcgtg aagcattcgg ttgccgtgag caggataaaa
    19861 ttgtcggttt tctctacctc ggcacgccgc agttgaaagc atctacgtcg attaacgtcc
    19921 cggacccgac gccgtttgta acttatttct gataattgtc gcgaaactgt ctggattgtg
    19981 agcaaaggcg caggaattca gacactctca cttatcactt cacggaatga gggttaccat
    20041 acccggattg cattgacagg agatgtccat gagcgagaac tcgattcgtt tgacccaata
    20101 cagccacgga gctggttgcg gctgtaaaat ttccccaaaa gtgttggaaa ccatcctgca
    20161 cagtgagcag gcgaagtttg ttgatctgaa tttgcttgtg ggtaatgaaa cccgcgacga
    20221 tgcggcggtg tacgatctgg gcaatggcac cagcgttatc agtaccaccg acttctttat
    20281 gccgatcgtt gataatcctt tcgattttgg ccgcattgcg gcgactaacg ccatcagcga
    20341 tatcttcgcg atggggggca aaccgattat ggcgattgcg atcctcggct ggccgattaa
    20401 caaactttcc ccggaaattg cccgcgaagt aaccgaaggt ggacgctatg catgccgtca
    20461 ggcgggtatt gcgctggctg gcggtcactc catcgatgcg ccggagccga tttttggtct
    20521 ggcggtaacg gggatcgtac cgaccgagcg ggtgaagaaa aacagtaccg cacaagccgg
    20581 atgcaaactg ttcctgacga aaccgctggg gatcggcgtt cttaccacgg ctgagaaaaa
    20641 atcactgttg aaaccagaac atcagggact ggcgacggaa gtgatgtgcc ggatgaacat
    20701 cgcaggcgcg tcctttgcca acatcgaagg cgtaaaagcg atgaccgacg ttacgggctt
    20761 tggtctgctg ggccacttga gcgaaatgtg tcagggggct ggtgtgcagg cacgcgtcga
    20821 ctatgaagcg atcccgaaac tccccggtgt tgaagagtac attaagttgg gcgcagtacc
    20881 tggcggcact gaacgtaact ttgccagcta cggtcatctg atgggtgaaa tgccgcgtga
    20941 agtgcgcgat ctgctgtgcg atccgcaaac ttctggcggt ttgctgctgg cggtcatgcc
    21001 ggaagcagaa aatgaggtta aaactacagc cgccgagttt ggcattgaac tgacggcgat
    21061 tggcgaactg gtgccagcgc gcggcggtcg tgccatggtt gagatccgtt aatttaatgc
    21121 ggttgtttat tgccgaaaaa ccgagtctgg cgcgcgccat tgctgatgtc ctgcccaaac
    21181 cgcaccggaa aggcgatggc tttatcgagt gcggtaatgg tcaggtggtg acttggtgta
    21241 tcggtcacct gcttgagcag gcgcagccag acgcctacga cagccgctat gcgcgctgga
    21301 atcttgcgga tttgccgatt gtcccggaaa agtggcaatt acagccacga ccctccgtga
    21361 ccaaacaact taacgtcatc aaacgcttcc tgcatgaagc cagcgaaatc gttcacgccg
    21421 gggacccgga tcgtgaaggg caattgctgg tggatgaagt gctggactat ctgcaactgg
    21481 caccggaaaa gcgccagcag gtgcagcgtt gcttgataaa cgacctgaac ccgcaggcgg
    21541 ttgagcgggc gatcgaccgt cttcgttcca acagtgagtt tgtgccattg tgcgtttctg
    21601 cgctggcgcg agcgcgtgcc gactggctgt acggcatcaa tatgacccgt gcgtatacca
    21661 ttctcggtcg caatgccggt tatcagggcg tactttccgt gggacgcgtg cagacacccg
    21721 tgctcgggct ggtggtgcgc cgcgatgaag agattgaaaa cttcgtggcg aaagacttct
    21781 ttgaagtcaa agcacatatc gtgacacctg ccgatgagcg gtttaccgct atctggcaac
    21841 cgagcgaagc gtgtgaaccg taccaggatg aagaagggcg cttgttacat cgtccactgg
    21901 cggagcatgt ggttaaccgc attagtggtc aaccggctat tgtcaccagc tataacgata
    21961 aacgggaatc agaatccgcg ccgctgccgt tttcgctttc ggcgttgcag attgaagcgg
    22021 caaaacgctt cggtctgagc gcgcagaacg tgcttgatat ctgccagaag ctgtacgaaa
    22081 cgcacaagct aatcacttat ccgcgttccg attgtcgcta tttgccagaa gaacattttg
    22141 ccggacgcca cgcggtgatg aatgccatca gcgttcatgc accggatctg ttgccgcagc
    22201 cagtggtaga tccagatata cgcaaccgct gttgggatga caaaaaggtc gatgcgcacc
    22261 acgccatcat cccgaccgca cggagttctg cgatcaacct gacggagaac gaagcgaagg
    22321 tctataacct gattgcccgt caatacctga tgcagttctg cccggatgcg gtgttccgca
    22381 agtgtgttat cgaactggac attgccaaag gcaaatttgt cgctaaagcc cgttttcttg
    22441 ctgaagcagg ctggcgcacg ctgttaggca gcaaagagcg cgatgaagaa aacgacggca
    22501 cgccattgcc tgtggtggcg aaaggcgatg agttgctgtg tgaaaaaggt gaagtggtag
    22561 agcggcaaac ccagccgccg cgccatttta ccgatgcaac actgctttcg gcgatgaccg
    22621 ggatcgcgcg ctttgtgcag gacaaagatc tgaaaaaggt ccttcgtgcg accgatggtc
    22681 tggggacaga agcaacccgt gccgggatta ttgaactgct gttcaagcgt ggttttctga
    22741 ccaaaaaagg gcgctatatc cactccaccg acgccggaaa agcgttattc cattcgctgc
    22801 cagaaatggc gacgcgaccg gacatgaccg cgcactggga atcggtgctg acgcaaatca
    22861 gcgaaaagca gtgtcgctat caggacttta tgcagccgct ggtggggacg ctatatcagc
    22921 ttattgatca agccaaacgt acgccggtgc ggcagtttcg cggcattgtg gctccgggca
    22981 gtggtggcag tgctgataag aaaaaggctg caccgcgtaa acgtagtgcg aaaaaaagtc
    23041 cgccagcaga tgaagccgga agcggggcga tagcgtaagc gagcgaatct ttcgtgctat
    23101 tcgagtcata ttctgaaata tccagcggat caagaaaatt cgttggatat ttttttgcat
    23161 ggataaaatt atcgcctcta aagtatgtaa taacagggaa tgtgatgaaa aaagttttat
    23221 tgcaaaacca tcctgggagc gagaagtatt cttttaatgg ctggggaata tttaatagta
    23281 attttgaacg ggtgattaaa gaaaataagg ccatgctgct ttgtaagtgg gggttttatt
    23341 taacatgtgt tgtcgctgta atgtttgtat tcgcagcgat aacatccaac ggtttgaatg
    23401 aaagaggcct gattaccgcg ggatgctctt ttctttatct attaattatg atggggctta
    23461 ttgttcgggc cggttttaaa gcaaaaaaag aacaactgca ttattatcag gctaaaggta
    23521 ttgagccgct cagtatcgag aagttacagg cgctacaatt gatcgcacct tatcgattct
    23581 atcataagca atggtctgaa acgctagagt tctggccgcg aaagcctgaa cctggcaaag
    23641 ataccttcca atatcatgtg cttccttttg actcgatcga tatcatcagt aaaagacgcg
    23701 agtctttaga gaatcaatgg ggtatcgaag atagcgaaag ttattgtgcc ttaatggagc
    23761 attttctttc tggcgaccat ggagccaata cctttaaagc aaacatggag gaagccccag
    23821 agcaggttat cgccttgttg aataaatttg ctgtttttcc ctcagactat atctctgatt
    23881 gcgctaataa tcgctccggt aaatcctcgg cgaagctaat atgggcggcg gaattatcat
    23941 ggatgatctc gatatcaagc acagcttttc aaaacgggac aattgaagaa gaactggcct
    24001 ggcattatat aatgcttgct tctcgaaagg cgcacgagtt gttcgaaagc gaagaagatt
    24061 atcaaaaaaa tagtctaatg ggatttcttt actggcatat ctgctgctat cgcagaaagt
    24121 taacggatgc tgaacttgaa gcgtgttatc gttacgacaa gcagttttgg gagcactaca
    24181 gtaagaaatg ccgttggccc ataagaaatg ttccctgggg agcatcatcc gttaaatact
    24241 cataaacgcc tgaaattttg ccgggggcgc tgcgcttgac cggcctacat tagagcgtaa
    24301 ctgcaatgaa ttgtgcccat ctgtaggcct gataagcgta gcgccatcag gcatttacaa
    24361 cttaaatcac accctgcgcc agcatcgcat cggcaacctt cacaaaaccg gcaatgttcg
    24421 cgccctgcac gtagttggtt tgctcacctt caccaccatg ctcaacacag gcatggtgga
    24481 tatccagcat gatgtgatgc aaacgtgcgt caactttctc ggctttccag cccaggcgcg
    24541 cagcgttttg tgccatttcc aggcccgacg ttgctacacc gccagcatta gccgctttac
    24601 ccggtgcaaa cagtacgcct gcctgctgga acagttcagt cgcttcgatg gtggtcggca
    24661 tatttgcccc ttcagcgacg gctttaacgc cattggcgat aagctgatgc gcggcgtcaa
    24721 catccagttc gttctgggtg gcgcaaggca gggcgatatc aaccggtaca gaccacggct
    24781 gttggccttc gagatagacc agaccaaatt ctttggcgta atctgccact cgaccatcgc
    24841 ggctggcttt gatttcgata agacgtgcca gtttctcttt cgtgaatccg ctttcatcaa
    24901 ctacagtgcc gctggagtct gacgcagtga tcacacgagc accaaattcc atcgcttttt
    24961 cgatagcgta ctgggcgacg ttgccggagc cagaaacgga aacgctcatc ccttcaaaac
    25021 ccataccgtg gcgttttaac attgcttctg tgaaataaac cagaccgtag ccggtagctt
    25081 ccgggcgaat aagactaccg ccaaatgaaa ggcccttacc ggtgaagacg caggcggtat
    25141 tgttggagag ctttttcatc atccccgcca taaagccgac ttcacgacca ccaacgccga
    25201 tatcacctgc cggaacgtcg gtatccgcgc ccaggtggcg atacagttca gtcatcagcg
    25261 tctggcaaaa acgcatcact ttaccttcgc tttttccttt cggatcgaaa tcgctgccgc
    25321 ctttaccacc gcccatcggc agggtagtca gggcattttt gaaggtttgt tcaaagccga
    25381 ggaatttgag aatggaaagg ttaactgacg gatggaagcg cataccgcct ttgtacgggc
    25441 cgatggcaga gctgaactgc acacgccatg cacggttgac ctgtatctgg ttgcgatcat
    25501 caacccatac cacgcgaaac tggatcacgc gctccggttc aaccagacgc tccagtaatg
    25561 acatctggcg atattttgga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac
    25621 gaacggcttg cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg
    25681 actccagaga atatgtctga tccatagata taaaaacctt atatattaat acgattgtgc
    25741 ttatgttgtt tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg
    25801 taagcaagaa aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa
    25861 atgcgataac tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca
    25921 ataacgttaa ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat
    25981 taattgcact ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag
    26041 tgttcagttc aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc
    26101 aaaattattc tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg
    26161 atgacaaaac ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc
    26221 taataatgga atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca
    26281 gaccagcgcc tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc
    26341 atgaagatgg ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac
    26401 agttgcttcg atgtccaatt cttcgtttaa ctcacgcacc agcacctgct gctggctttc
    26461 atccagctcg actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg
    26521 gcgttgcgcg agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat
    26581 tttcataaat tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc
    26641 aatcagaaag gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga
    26701 ttaaaccgtt gttgcatcag tgcgtgcggg ttcttgataa accgggcatt acgccagatg
    26761 gtttaacatt agtgggattt gccatcggcg tgctggctct gccgtttctg gcgctgggct
    26821 ggtatctggc ggcgctagtc gtcattttgt tgaacaggct gcttgatggt ctggacggtg
    26881 cgctggcgcg gcgcagaggg cttaccgacg cgggcggctt tcttgatatt tctctcgatt
    26941 ttctctttta cgcgctggtg ccgtttggct ttattcttgc tgcaccggag caaaatgcgc
    27001 tggcaggcgg ctggttgttg tttgcgttta tcggcacggg cagcagtttt ctcgcctttg
    27061 ctgcactggc agcaaaacat cagattgata accccggtta tgcacataag tcgttttact
    27121 atctgggggg attaaccgaa ggcacagaaa cgatcttact gtttgtgctg ggatgtttat
    27181 tccctgcatg gtttgcgtgg tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct
    27241 ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct ccagcgtcag taattgcaat
    27301 gttatttgct actgcccggg ccgcgttcac cggttgcaac cggatttttt ggatcgctgc
    27361 tccattcgta ccagccgccg tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt
    27421 acataaaggt ttcggacgcg cgccagccgg tgccgcagta gaatgaaact tgctgatctg
    27481 gtttgatatt ccatgctttc cacatagcgg taatatcatc ggcgctacgc atggtgccat
    27541 ccgggttata gaaatcttcc atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc
    27601 cggctatttc accttttggt ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg
    27661 gccacgaacg aatgctcacc agcgatgcat cctggcgatg cagcagtcca cgggcctgtt
    27721 ccatatcgag catcaactgc ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt
    27781 tcactttcgg tggcgttcct cgctcaacag gcagtcccgc gtcggaccag gtttgccagc
    27841 cgccatccag cagacgcaca tctttcacgc ctgcgtagag cataatttgc gccacacgcg
    27901 ctccagcgta tacgtcacgc ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt
    27961 ttgccagcat cgcttttagt tgttcatcag aaactttgtt ccacagcggt tcgctttcca
    28021 cttcgttggt atcgatgtag tcagcgctag gaatatggct gataaggtaa agcttaggag
    28081 cgccctaggc cgcttcaatg actttccagt caccgacagg tttcgccgta acctctttac
    28141 cttgttgcag gtcatgcagc cattgcggat aaaccagctg ctcaaagtgc ggcagttttt
    28201 gtagacggga aggttcgctt agcgcgtcac tcagaatgga gatatgcgta aaacctgctt
    28261 tttgcagtcg cgttttgacg gcgtcgacat ctttgccatt accgtacagc gccaccgggg
    28321 catcggcttt caggttatgt tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt
    28381 cgagccagct agcagagagg tttaaggcgg caggttcatg accagaaggg ccatttaagg
    28441 tttgtggcca gccgttataa aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt
    28501 gctgaagttg gtcaagtgta agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag
    28561 ccagccctaa agccattgcc agcgcggtca tttgagaaac acgtttcatc gaaaatcccg
    28621 acattgtagt tttgagtgcg ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc
    28681 gcagtttgtt gtaattttct gaccactgcg ccatatccag aacagaacta tcagcaggaa
    28741 catcctggag atcgtgcgtt acctgaacga cggggatcgc cagggcgcga acttcgctga
    28801 acacccactg gcgaaaatta tcgcgcagag ccacatcaag acggctgaat ggctcatcca
    28861 ggagtaacgc ttttggctgg gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac
    28921 cgccagacaa agtggcagga tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg
    28981 catcattcac ggcattacgt cgggcattcc ccttaagtgt cgccggtagc gccagcagta
    29041 aattttgccc gacgctgaac tggtcaaata acagtgcatc ctgaaaaaga atgccaatct
    29101 gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt gagccatagc tcacctgtac
    29161 aagaaaactg tccggccagt gcaccaatca tccatgaaaa cagagtggat tttccacagc
    29221 cagacggccc cattaacgtg acgatgtcac ctttatcaac cgtaaagtta acgtttgtca
    29281 gcaagcggct ttctggtaaa cgtagcgaaa catttttcac gcagagcatt agcggattcc
    29341 ttgtctgaca taacctaccc attttgcgac taacgcggtc agggcaaaaa taataagcgg
    29401 taacaacagt tgccataacg cctgggcggc gagaataccg ttgctgccgc cgctgcttaa
    29461 tgccaccgcc tcagtggtga gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat
    29521 atactgcgca atactgactg aaaatcccac cgcgaaggca gtcagcacag ggcgcaacat
    29581 gagtgggcat ttcacgtaaa agaagatttt ggcccgcgac cagcccagtg tttgcgcaat
    29641 caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc aggataaaca gcatccacgg
    29701 cattacccac agcagatgcc cccagaccac cgccgtccag cttccatcca ggttcagcca
    29761 tagcgccagc gtgtactggc ccgccaccag tggcagagcg ggcaatagaa tgggcaacca
    29821 tagccacaac tggcgacgct gtgcccccca ttccagccac agcagtaaca ggagcaaagc
    29881 gatgaatgtt gctaccagcc ccattgtcag actgttgatg agtgcttcac tattgatcgt
    29941 cgactgatcc gcgaggatcg ccagcagaac cacacacagc acaccggtta agggtaaaaa
    30001 actcgccagc gtagtgcctg gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg
    30061 gggaatagtg cgccgccagc tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca
    30121 gactggcgag tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc
    30181 taattactgc cagcgtcggt ggattacctg gcccaagaat aatcgccaca tccacgaccg
    30241 aaagcgacca ggcaacaatc gccagcatcg ccattgccag cgaaggcgcg acggagggca
    30301 acagcagcca gttcaggcat tgccagcggc tgtagcccag cgaatccagc acaatgacct
    30361 gctgcaacag ccgtttttcg ctcaatactg ccactaagat ccacagcaga aatgcgcttt
    30421 ctttcactgc aagggtgagg cccagaccga tccaaatcgg tccattggcg gagtgaaata
    30481 cgggaaatag tcatatagca gccctccgtc agcaaagagc agcagggcgc tggtggcaaa
    30541 agccacatgg ggaatggcga gcagccacgg cagacgggcg cacatacgct gccatttcgg
    30601 cccaggccat agcgccacaa tcaccagcag ggcaatcaac aatgccccga ccgccgcgat
    30661 ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc
    30721 ctgccagcgt gtcaacgaca acgcaggcga tatcagcgtg agagctgccg ggatcagcgg
    30781 tgcataaatc accgccacca tcgcccacag caaaaagatt aatgcgtacc gtaacggcgt
    30841 aaccattctt gttccagtgc attcacccat cctgcgtgcg gttcagccag taccggcggc
    30901 agatcctgcg gcattcttga ttgcaatatt tcgcgctgcc cgtcaggcag tttttgcgga
    30961 tcgagaacag aaggatcgcc ccagacaacg ggatctgctt tacgcagttg cgcatcgggt
    31021 gagagcagga aattggcaac taccttcgcc gcagcactgg cattcgcgtt ggctggagtg
    31081 gtgacgaaat gcacgttgcc aatcatcccc tcgcgaaagc caaaactgta actgcttgcc
    31141 ggcaaatcgc cgctggcgat tttttgctgc gcatgtgcgg ggttaaaggt cagcgacagg
    31201 cgcaatgtgc cggctttcag cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt
    31261 ccttcgcgcc acaaatacgg atgcagtgca tcaagatatt gctacaaggg cgcagtgaca
    31321 cgggcgaaag tcgcatcgtc cggcgcttct tttaatgcgg cgggatctgg cgtcagcata
    31381 atcagcaact gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg ataagtaacc
    31441 gtgccgggat tggctttagc aaactccagt aaggcttgcg gcgtttgtgg tggctgtggc
    31501 gtaacatcgc ggcgggcgat aaacgtcagt tgtgcgccgc cccagggcga ttccgcacct
    31561 tgtgtcggca ctgaaaaatc tccccgcacc ggcagctgtg tgtcgacata gcgccagttg
    31621 ggcagagtct ccgcccagcc cgtttgtaat aaattggcct cttt
//
LOCUS       DAJTNW010000045        31188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNW010000045 DAJTNW010000000
VERSION     DAJTNW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            389..2047
                     /locus_tag="OO179_002123"
     CDS             389..2047
                     /locus_tag="OO179_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5532178.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO179_002124"
     CDS             complement(2044..3000)
                     /locus_tag="OO179_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5532179.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO179_002125"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO179_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5532180.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO179_002126"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO179_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5532181.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO179_002127"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO179_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5532182.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO179_002128"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO179_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5532183.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO179_002129"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO179_002129"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5532184.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OO179_002130"
     tRNA            complement(9859..9946)
                     /locus_tag="OO179_002130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO179_002131"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO179_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5532185.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO179_002132"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO179_002132"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5532186.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO179_002133"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO179_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5532187.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO179_002134"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO179_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5532188.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO179_002135"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO179_002135"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5532189.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OO179_002136"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OO179_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5532190.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OO179_002137"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OO179_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5532191.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OO179_002138"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OO179_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5532192.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OO179_002139"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OO179_002139"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5532193.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OO179_002140"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OO179_002140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5532194.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="OO179_002141"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="OO179_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="OO179_002142"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="OO179_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5532195.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="OO179_002143"
     CDS             28043..28834
                     /locus_tag="OO179_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5532196.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="OO179_002144"
     CDS             complement(28870..29496)
                     /locus_tag="OO179_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5532197.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="OO179_002145"
     CDS             29811..30959
                     /locus_tag="OO179_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532198.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7687 a         7643 c         8379 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcagga
//
LOCUS       DAJTNW010000046        30014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNW010000046 DAJTNW010000000
VERSION     DAJTNW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO179_002146"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO179_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5532199.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO179_002147"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO179_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5532200.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO179_002148"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO179_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5532201.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO179_002149"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO179_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5532202.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO179_002150"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO179_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5532203.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO179_002151"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO179_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5532204.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO179_002152"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO179_002152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5532205.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO179_002153"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO179_002153"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5532206.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO179_002154"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO179_002154"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5532207.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO179_002155"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO179_002155"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5532208.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO179_002156"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO179_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5532209.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO179_002157"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO179_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5532210.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO179_002158"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO179_002158"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5532211.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO179_002159"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO179_002159"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5532212.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO179_002160"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO179_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5532213.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO179_002161"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO179_002161"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5532214.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO179_002162"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO179_002162"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5532215.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO179_002163"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO179_002163"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5532216.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO179_002164"
     CDS             21622..22212
                     /locus_tag="OO179_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5532217.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO179_002165"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO179_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5532218.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO179_002166"
     CDS             22684..23379
                     /locus_tag="OO179_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5532219.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO179_002167"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO179_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5532220.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO179_002168"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO179_002168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5532221.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO179_002169"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO179_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5532222.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO179_002170"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO179_002170"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5532223.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO179_002171"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO179_002171"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5532224.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7822 c         8256 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccat atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gaga
//
LOCUS       DAJTNW010000047        29787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNW010000047 DAJTNW010000000
VERSION     DAJTNW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            57..851
                     /locus_tag="OO179_002172"
     CDS             57..851
                     /locus_tag="OO179_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5532225.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="OO179_002173"
     CDS             873..2282
                     /locus_tag="OO179_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532226.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="OO179_002174"
     CDS             2260..3039
                     /locus_tag="OO179_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5532227.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="OO179_002175"
     CDS             3036..3896
                     /locus_tag="OO179_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5532228.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="OO179_002176"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="OO179_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="OO179_002177"
     CDS             complement(4636..4896)
                     /locus_tag="OO179_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5532229.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="OO179_002178"
     CDS             complement(4887..6158)
                     /locus_tag="OO179_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5532230.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO179_002179"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO179_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5532231.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="OO179_002180"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="OO179_002180"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5532232.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="OO179_002181"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="OO179_002181"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5532233.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="OO179_002182"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="OO179_002182"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5532234.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="OO179_002183"
     CDS             11500..11652
                     /locus_tag="OO179_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5532235.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="OO179_002184"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="OO179_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO179_002185"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO179_002185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5532236.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="OO179_002186"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="OO179_002186"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5532237.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="OO179_002187"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="OO179_002187"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5532238.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="OO179_002188"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="OO179_002188"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5532239.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="OO179_002189"
     CDS             17364..17687
                     /locus_tag="OO179_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5532240.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="OO179_002190"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="OO179_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5532241.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="OO179_002191"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="OO179_002191"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5532242.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="OO179_002192"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="OO179_002192"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5532243.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="OO179_002193"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="OO179_002193"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5532244.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="OO179_002194"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="OO179_002194"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5532245.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="OO179_002195"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="OO179_002195"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5532246.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="OO179_002196"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="OO179_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5532247.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="OO179_002197"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="OO179_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5532248.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="OO179_002198"
     CDS             complement(24262..24669)
                     /locus_tag="OO179_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5532249.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="OO179_002199"
     CDS             24816..25409
                     /locus_tag="OO179_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5532250.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="OO179_002200"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="OO179_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="OO179_002201"
     CDS             complement(26629..27192)
                     /locus_tag="OO179_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532251.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27345..27596
                     /locus_tag="OO179_002202"
     CDS             27345..27596
                     /locus_tag="OO179_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5532252.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27598..29694
                     /locus_tag="OO179_002203"
     CDS             27598..29694
                     /locus_tag="OO179_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5532253.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7297 a         7378 c         8055 g         7057 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggcgtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggctg ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc agccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacactcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cttggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcaatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaacaccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggatcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcaggggcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccggata atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc tcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatggaca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct cgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaatgat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg atgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcacgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgt atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttaccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tgtgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt acagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgcgcgg gtaattttgt aggttaaagg aaagatgaga caaagagaag
    27361 atagtgaccg cgattggcct tcgctgaaga ttcagctggc gctcaaagat aacgggtggt
    27421 cttccttccg ggagctttca ctccatgctg gttatgctca ggattcactg aaagcggtag
    27481 ttcgcacacc aaacaaaaaa tacgaacgcg taatttctga cgctataggt gtaccgccag
    27541 aaatcatctg gcccagccgg tatcgtaacg atggatttat caggaaggcg tcgtgatatg
    27601 cagtcgtacg ttaccgtaaa tgacctgctc ggcgtaccag gaatgccagc caccacaaaa
    27661 ggcatccgac aggcgttaca gcgtttttct cgtgatcttg gtgacgttag tcgccgtcgg
    27721 gaaggcacaa aagccattga gtaccacatt gattgtctgc ctgaaataac acgaaaggca
    27781 ctgcgtgaac gctatgtgga acagctggtg gctacagaga acaatgtttc tgaagtgaaa
    27841 gctgttacca gaaaaacacg caatcctgac gctgtccagg caatcgaagc atatcgcggt
    27901 tctccacaac tgatggaaga acgcctgaat gcgctgaccg aaaaccagcg ctgggtatct
    27961 gaagcaagag ctgcgctggt ggtggaagtg ctgaagctgg aaagcgccgg taaccccggg
    28021 cgactgaaag ccattaactt tcttgttgaa aaagcccgta aaggtgagct gccggagcgc
    28081 ctgcaacagg ccgcagttaa cgccaatgca aaacgtggcg ctaatcgtac tatcagccgc
    28141 gatccacttt accagtgggt tctgaaatac aaccagtcgc agaacgccgc cgaacggctg
    28201 ctgttactgg caccgggaaa acgcgacgaa ataaaaccgg aagaaatcag ctggctgcct
    28261 gaatttctgg cgcagtatcg ccaggtgaac ggcagaccca tgtcagaagc ctacgaggat
    28321 tttgtcgcag aatggcagcg tcgccatgcg gacgaaccgt acatgctgga ggtgatgcct
    28381 tcctatgatg ttgtccggta tgccatgaaa aaactgccgg aagtggtgaa acaaaaaggg
    28441 cgcgttaccg gcagtgaata ccgtcagctt gaaggattca cccgccgcga ctggacagct
    28501 atgccggtga attatgtctg gattggtgac ggtcacggta tgaagctgaa atgtgcgcat
    28561 ccgatccacg ggcgtccatt ctcaccggaa gtcacgtttg tgatcgatgg tggaacacgg
    28621 tttgttgttg gctggagtct tgaccttgcc gaaaacgtgt tcgccgttgc cggtgcgata
    28681 cagcacggca ttcgcaacca cggaaaaccg ttcctgtatt actcggataa cggttccggt
    28741 gaaacggctg acatgctgga caaagaggtc gtgggtattc tgccacgcct ggggattaaa
    28801 caccctaccg gtattgccgg taatccacag ggacgcggca ttattgaacg cctgaaccgg
    28861 acactgccga tgcgtatagc caggaggtat cgcacctatt ttggcaaggg tgctgaccgg
    28921 gaatcgttgc gtgtacttaa ccgtgatctg cgttcagcgt ttaacgccct gcaacaggac
    28981 aagcctctga acgaccgcca gaaagcggca atgcgcgagc tgccatcgtg ggcggagctt
    29041 attgaggcta ttcgtgaagg cgtggaatgg tacaacaacc gcccgcattc tgagctgccg
    29101 atgaaaccga acggacggca ttacagcccg acagagttca gaaaaaaacg ccaggcagaa
    29161 gaagacaccg aaattgagtg gctgagtgat cttgagctgc gcgacatgtt ccgcccaatg
    29221 gtggaacgac cggtaagacg ctgcgaaatt cagtggctga acaatattta ctacgcgccg
    29281 gagttgcgtg acgaacacgg tcgcaaagtg cttatcagtt atgacattca tgacgccgag
    29341 cgaattacgg tacgtcgcaa ggacggcagc tttatctgtg aggcgatatg gaacggcaat
    29401 aaacgcgcag cctttgctgt cagtgctgaa tatcacaaac aacagcagcg tattaaagga
    29461 atgcgcaaac gcgctgagga aaaaattcgt gatgctgaag atgaaggtat tcagattctg
    29521 gaacacaaac aggctgaacc ctggctgagt aatgtttatc gcccggttgg taatgtggtg
    29581 gcagttcagc aaccggaata tgaagaagag catgacgaag aatttgaacg cgatttccgg
    29641 ctggggatgc aaaaactttt tgccatgcag gaagaagatg atccgctggc ctgaataaaa
    29701 aaacagtccg cgggaacgga ctgttaaata aaggaaacaa agctgatttg aaataaccga
    29761 ggaaatacta tgactgatat tgatgat
//
LOCUS       DAJTNW010000048        29638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNW010000048 DAJTNW010000000
VERSION     DAJTNW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..188)
                     /locus_tag="OO179_002204"
     CDS             complement(<1..188)
                     /locus_tag="OO179_002204"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5532254.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO179_002205"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO179_002205"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5532255.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OO179_002206"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OO179_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5532256.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OO179_002207"
     CDS             3865..4146
                     /locus_tag="OO179_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5532257.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4963
                     /locus_tag="OO179_002208"
     CDS             4439..4963
                     /locus_tag="OO179_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5532258.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5044..5718
                     /locus_tag="OO179_002209"
     CDS             5044..5718
                     /locus_tag="OO179_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5532259.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5734..8214
                     /locus_tag="OO179_002210"
                     /pseudo
     CDS             5734..8214
                     /locus_tag="OO179_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8230..9263
                     /locus_tag="OO179_002211"
                     /pseudo
     CDS             8230..9263
                     /locus_tag="OO179_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OO179_002212"
     CDS             complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OO179_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5532260.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OO179_002213"
                     /pseudo
     CDS             complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OO179_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10872..11144
                     /gene="rcnR"
                     /locus_tag="OO179_002214"
     CDS             10872..11144
                     /gene="rcnR"
                     /locus_tag="OO179_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5532261.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11236..11332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11367..12155
                     /gene="thiM"
                     /locus_tag="OO179_002215"
     CDS             11367..12155
                     /gene="thiM"
                     /locus_tag="OO179_002215"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5532262.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12152..12952
                     /gene="thiD"
                     /locus_tag="OO179_002216"
     CDS             12152..12952
                     /gene="thiD"
                     /locus_tag="OO179_002216"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5532263.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13017..13838
                     /locus_tag="OO179_002217"
     CDS             13017..13838
                     /locus_tag="OO179_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5532264.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13886..14632
                     /locus_tag="OO179_002218"
     CDS             13886..14632
                     /locus_tag="OO179_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5532265.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14606..15571)
                     /locus_tag="OO179_002219"
     CDS             complement(14606..15571)
                     /locus_tag="OO179_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5532266.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15568..16571)
                     /locus_tag="OO179_002220"
                     /pseudo
     CDS             complement(15568..16571)
                     /locus_tag="OO179_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16568..17845)
                     /locus_tag="OO179_002221"
     CDS             complement(16568..17845)
                     /locus_tag="OO179_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532267.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18102..19154
                     /gene="fbaB"
                     /locus_tag="OO179_002222"
     CDS             18102..19154
                     /gene="fbaB"
                     /locus_tag="OO179_002222"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5532268.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19462..20316
                     /gene="gatY"
                     /locus_tag="OO179_002223"
     CDS             19462..20316
                     /gene="gatY"
                     /locus_tag="OO179_002223"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5532269.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20345..21607
                     /gene="gatZ"
                     /locus_tag="OO179_002224"
     CDS             20345..21607
                     /gene="gatZ"
                     /locus_tag="OO179_002224"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5532270.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21617..22069
                     /gene="gatA"
                     /locus_tag="OO179_002225"
     CDS             21617..22069
                     /gene="gatA"
                     /locus_tag="OO179_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5532271.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22100..22384
                     /gene="gatB"
                     /locus_tag="OO179_002226"
     CDS             22100..22384
                     /gene="gatB"
                     /locus_tag="OO179_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5532272.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22388..23743
                     /locus_tag="OO179_002227"
     CDS             22388..23743
                     /locus_tag="OO179_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5532273.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23791..24831
                     /gene="gatD"
                     /locus_tag="OO179_002228"
     CDS             23791..24831
                     /gene="gatD"
                     /locus_tag="OO179_002228"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5532274.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24937..25710
                     /locus_tag="OO179_002229"
     CDS             24937..25710
                     /locus_tag="OO179_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5532275.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OO179_002230"
     CDS             complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OO179_002230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5532276.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27097..27414
                     /locus_tag="OO179_002231"
     CDS             27097..27414
                     /locus_tag="OO179_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532277.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OO179_002232"
     CDS             complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OO179_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5532278.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29264..29595)
                     /locus_tag="OO179_002233"
                     /pseudo
     CDS             complement(29264..29595)
                     /locus_tag="OO179_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7702 a         6879 c         7477 g         7580 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
     4801 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
     4861 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
     4921 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
     4981 ttctccaggt aataaacatg gctgctatcc ccatggcggc tttttaattt aagaggcatt
     5041 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
     5101 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
     5161 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
     5221 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
     5281 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
     5341 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
     5401 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
     5461 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
     5521 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
     5581 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     5641 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     5701 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     5761 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     5821 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     5881 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     5941 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     6001 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     6061 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     6121 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     6181 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     6241 tcgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     6301 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     6361 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     6421 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     6481 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     6541 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     6601 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     6661 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     6721 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt
     6781 gaaggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac
     6841 ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctctga
     6901 acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa
     6961 caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg
     7021 agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg
     7081 acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat
     7141 atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc
     7201 aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga
     7261 gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg
     7321 cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag
     7381 aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct
     7441 cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat
     7501 aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg
     7561 agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg
     7621 gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt
     7681 gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa
     7741 acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat
     7801 cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat
     7861 cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa
     7921 attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag
     7981 ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc
     8041 aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc
     8101 accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc
     8161 acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca
     8221 ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg
     8281 agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat
     8341 tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc
     8401 gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg actttataac
     8461 gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca
     8521 aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat
     8581 gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca
     8641 ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta
     8701 ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat
     8761 gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa
     8821 caggacgatt tattaattac tttagacagt attcccgttt cacaattacc agccacaggt
     8881 aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta
     8941 ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta
     9001 accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa
     9061 gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt
     9121 taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag
     9181 ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat
     9241 taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa
     9301 taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa
     9361 atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg
     9421 ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac
     9481 tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa
     9541 tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc
     9601 gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc
     9661 aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca
     9721 tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt
     9781 cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg
     9841 gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga
     9901 gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca
     9961 acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg
    10021 cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc
    10081 caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata
    10141 tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca
    10201 aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg
    10261 ctcgtgcatg ggcatcctga tactcgccat attcgtgatg gtgatgatgt ccttggtcgt
    10321 ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg atggtgatga tgctcataat
    10381 catgcccgtg catattctcc agccagttgc gttcgccgcg ctaggtacgc cagaacatcc
    10441 agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt
    10501 gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg
    10561 tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg
    10621 cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac
    10681 caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa
    10741 attcggtcat gataataatt cttagtatta attcggcaat ctgattctac tcccccccag
    10801 tacctgatac taccccccca gtagattaat agtgctatga tttttcatat tcttgttaac
    10861 caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg cgtgccagta
    10921 agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac gaatgcgctg
    10981 cagttttaca acagattgct gctatccgtg gagcggtaaa cggtctgatg cgggaagtga
    11041 ttaaaggtca tctgacggaa cacatcgttc accaggggga tgagctaaaa cgtgaagaag
    11101 atctggatgt cgttctgaag gtgctggatt catatatcaa ataatttatt aacgcgattg
    11161 taaaactgcc gtttttcctc gtttacaacg ggggcgctgg acattaccat cctcctctgc
    11221 gatttatcat cgcaaccaaa cgactcgggg tgcccttctg cgtgaaggct gagaaatacc
    11281 cgtatcacct gatctggata atgccagcgt agggaagtca cggaccacca ggtcattgct
    11341 tcttcacgtt atggcaggag caaactatgc aagtcgacct gctgagttca gcgcaatctg
    11401 cgcacgcgtt acaccttttt caccaacatt cccctcttgt gcactgcatg accaatgatg
    11461 tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg tgcatcgcca gcgatggtta
    11521 tcgaaaccga agaggccagt cagtttgcgg ctatcgccag tgccttgttg attaacgttg
    11581 gcacactgac gcagccacgc gcacaggcga tgcgtgctgc cgttgagcaa gcaaaaagct
    11641 ctcaaacacg ctggacgctt gatccagtag cggttggtgc gctcgattat cgccgccatt
    11701 tttgtcatga acttttatct tttaaaccgg cagcgatacg tggtaatgct tcggaaatca
    11761 tggcattagc tggcattgct aatggcggac ggggagtgga taccactgac gccgcagcta
    11821 acgcgatacc cgctgcacaa acactggcac gggaaactgg cgcaatcgtc gtggtcactg
    11881 gcgaggtgga ttatgttacc gatggacatc gtatcattgg tattcacggt ggcgatccgt
    11941 taatgaccaa agtggtagga actggctgtg cattatcggc ggttgtcgct gcctgctgtg
    12001 cgttaccagg cgatacgctg gaaaatgtcg catctgcctg tcactggatg aaacaagccg
    12061 gagaacgcgc agtcgccaga agcgaggggc caggcagttt tgttccacat ttccttgatg
    12121 cgctctggca attgacgcag gaggtgcagg catgaaacga attaacgctc tgacgattgc
    12181 cggtactgat ccgagtggtg gtgcggggat tcaggccgat cttaaaacct tctcggcact
    12241 tggcgcttat ggttgctcag ttattactgc actggtggcg caaaataccc gtggcgtaca
    12301 gtcggtgtat cgcattgagc ctgattttgt cgccgcccag ctcgattcgg tgttcagcga
    12361 tgtgcgaatc gataccacta aaatcggtat gttggcggaa accgatattg ttgaagcggt
    12421 ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta ctcgacaccg ttatgctggc
    12481 aaaaagcggc gacccgctgc tttcaccttc ggcggttgct acgctgcgca gtcgattatt
    12541 gccacaggtt tcattaataa cgccaaactt gcccgaagct gccgccttgc tcgacgcgcc
    12601 acacgcgcgc accgaacagg aaatgctgga acaagggcga tcgctgttgg cgatgggctg
    12661 tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag caaagcccgg actggctgtt
    12721 tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt atgaccaaaa acacccacgg
    12781 cactggttgt acactctctg cggcgttggc tgcactacgc ccgcgccata caaactgggc
    12841 tgacaccgta caggaggcaa aaatctggct ttcatcggcg ttagcccagg ccgacacgct
    12901 ggaagttggt cacggtattg gtccggttca ccacttccac gcctggtggt gagcaccgta
    12961 ctatagctgc tttggttatt gtcagccttt ggcactgaca ttcagggatg aaaccaatgc
    13021 agctaagaat taccagtcga aaaaaactca ccgctctatt atgcgctctt gggctaattt
    13081 ccatcgttgc aatttatccg cgtcagacgg tgaatttttt ctattcgaca gcagttcaga
    13141 ttacagacta catccacttc tacggttatc gcccggttaa atctttcgct attcgcattc
    13201 ctgccagtta caccattcac ggattagatg tttcgcgctg gcaggagcgt atcgactggc
    13261 agcgggtggc aaaaatgcgc gacaacgata tccgcttaca gtttgctttt attaaggcta
    13321 cggaaggcga aaagctggtg gacccctatt tttcgcgtaa ctggcaatta agccgcgaaa
    13381 atggcctgct gcgcggggcg tatcattatt tctccccgtc ggtatccgct tcagttcagg
    13441 cgcgattatt tctgcaaacg gtggatttct cacaaggcga tttccctgcc gtgctggacg
    13501 tagacgaacg gggaaaatta tcggcaaaag aattacgcaa gcgggtaagt cagtggctaa
    13561 aaatggtcga aaaacgcacg ggaaaaaagc cgattattta ctcaggagcc gttttttatc
    13621 acaccaatct ggcgggctat ttcaatgaat atccgtggtg ggtggctcac tattatcaac
    13681 gtcgtccgga caatgacggc atggcctggc gcttctggca gcattccgac cgtggacagg
    13741 tagatggcat caatggtccg gtggatttta atgtgtttaa tggcacgggg atgagttgca
    13801 ggcattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata tactcccacc
    13861 agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga ttgcccaact
    13921 gaacgagcgt attttagcgg cggataacac gccgctttat attaagtttg ccgaaacggt
    13981 aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg gtgagcgtga
    14041 tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc aggcgctgga
    14101 agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca acatcttcga
    14161 atactcgttg aaagaagcga gaggtttttc tcagcaggtg gtattgtgcg ggaaaaagcc
    14221 cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc gaagaagttg cgcagcagct
    14281 ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt attcgctatg tcgatgaaga
    14341 agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg ttgatgccat
    14401 cggaatttct ctttatgact atttccgcag ccagcacatt tacccacaac gtacgcgttc
    14461 ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt tagatagcaa
    14521 aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc ctattgagta
    14581 cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt agttcctcgc
    14641 gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact gcattgccca
    14701 gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg ccacctgcat
    14761 gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga atgcagccag
    14821 aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc aacggcgcgg
    14881 caaatttctc ctgccattgc ttgccaagtg ttgttatctc tgcggataaa gcaaaacgtt
    14941 cggcggcaat ctcagcctct tgacgattga gtgacactaa aggtcgacag gccatgatcc
    15001 gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca aaaatcgata aacggcgtca
    15061 cgtcttgcag cttttccagc cattccacta acaattcgcc gcagggcgag gccagttgat
    15121 aaccggaaaa atagagcagg ttgccaggcg caacggttaa tcgtgccaac cattggcgat
    15181 tccattgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc ggctcaacca
    15241 gtgccagaca ccagccgtta tcgctttcgg cgttatcgat caggctgatt aacccctctt
    15301 tcgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc aaggcattac
    15361 ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag ccgccaacgt
    15421 taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca taagcgtcgg
    15481 cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg gttaattcag
    15541 gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac gatatttcgc
    15601 cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagct ccgcctttaa
    15661 cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa atcgccgtcg ccatagcacc
    15721 tatggtgtct gtgtcgccgc caagattggc gcacaggacg gcgcagcggt ttgggtcggt
    15781 ttgtgccagt tcaactagcg caatggcgca cggaacggac tcaatagtgc tggtgcctgc
    15841 gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat tacgcacaat
    15901 tttcagcgca atctccagac gtgccgccag tgaggcgctg aacgtggtgg tgcgtttttg
    15961 ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc gctgaccagc gttctccgtc
    16021 aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca gatcggattt
    16081 atgggtcggg ctggacgcca gcgccacatc atcaataaag gaatcaacat cacgcgctgg
    16141 aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggttacgc cgttgttttc
    16201 cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg aggtcggacc
    16261 tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag tcgagaatat tacgcccaat
    16321 cagattcgga tcgatcttgc cttcacattc cagtaacgca tccgccagac acaggccatc
    16381 gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt ctcctttggt
    16441 ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg tggccaaagc
    16501 tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc gagaatacgt
    16561 tctgttttca tttaacttcc ccttgtgtca acgcaatatc tcgatcatcg accttgatag
    16621 ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa
    16681 tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg tttaccggtt
    16741 cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg agcaaactgc
    16801 cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata tgcacggggg
    16861 cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt acgccgtgaa
    16921 gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgcacta ccgtaaataa
    16981 agaagccata gcagatcgca gcggtgacca gaccaagcaa taataccttt ttgataccaa
    17041 agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac tggccgagcg
    17101 tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg gcaaagatgt
    17161 aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg aggaagtttt
    17221 tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatgtcc attttgccgg
    17281 tgctttttgg cggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga gcagaacttc
    17341 cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc agcatttgcg
    17401 gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc acacgaatgc
    17461 gtgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta gtcagcgcaa
    17521 tggtcggcat ataggttagc gagtaggcca gcagtaacgg aaagaacccg gcaaaagtgg
    17581 tctgttgcgc agcgaaatac atcagcacag caccagcgaa catcaatacc gccagcactt
    17641 tctgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac aggatcgccg
    17701 caatggctgt gcaggcatac gaccagccaa tttctccggc actaaaaccg cttttactta
    17761 accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca aacatcatga
    17821 acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa tgtaaggtac
    17881 gcgatgacaa taccacataa taatgccttt tgaatacctt tgcggtgaaa acttgaccgc
    17941 gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat
    18001 ggaaaaactc gcccgtaacg actaggatta tgattgcccg caaaaggcgg gccaggacaa
    18061 tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    18121 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc
    18181 agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc
    18241 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    18301 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    18361 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    18421 actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca
    18481 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    18541 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga
    18601 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    18661 aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct
    18721 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc
    18781 tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    18841 ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa
    18901 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    18961 taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg
    19021 ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat
    19081 cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa
    19141 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt
    19201 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    19261 agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc
    19321 ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt
    19381 ttttgtgatc attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa
    19441 agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    19501 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    19561 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    19621 tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    19681 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    19741 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    19801 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg
    19861 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg
    19921 tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg
    19981 gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga
    20041 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta
    20101 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    20161 gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg
    20221 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    20281 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    20341 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    20401 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    20461 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    20521 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg
    20581 cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc
    20641 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    20701 taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga
    20761 aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg
    20821 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    20881 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    20941 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat
    21001 cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    21061 agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    21121 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    21181 agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    21241 gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    21301 gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact
    21361 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    21421 aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat
    21481 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc
    21541 gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    21601 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    21661 tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac
    21721 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    21781 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    21841 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt
    21901 atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg
    21961 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac
    22021 tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    22081 acaataaaaa gtactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    22141 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt
    22201 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    22261 atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    22321 ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    22381 gtgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct
    22441 gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    22501 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    22561 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    22621 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    22681 ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    22741 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct
    22801 ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta
    22861 taagctctgc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    22921 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    22981 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    23041 tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    23101 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    23161 gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    23221 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    23281 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat
    23341 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat
    23401 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    23461 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    23521 cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    23581 tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc
    23641 cggtttcatt attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc
    23701 gcgtggtttt actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga
    23761 ggttatcccc cttaacaatc aggagttttt atgaaatcag tagtgaatga tactgatggt
    23821 atcgtgcgcg ttgcaaaaag cgtcattcct gaaattaaac atcaggatga ggtgcgggta
    23881 aaaattgcca gctcgggctt atgtggttcc gatttaccca ggatatttaa aaatggtgca
    23941 cattattatc caataacgtt aggccatgaa tttagcggct atattgatgc ggtgggatcc
    24001 ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg tgccgttatt accctgtttt
    24061 acttgtccag agtgtttgaa agggttttat tcccagtgcg caaaatatga ttttattggc
    24121 tcgcggcgtg atggtggatt tgctgaatat attgtcgtta agcgaaaaaa tgtctttgct
    24181 ctacccacgg atatgcctat tgaggatggg gcttttattg agccgattac cgttggcctg
    24241 catgcttttc atttagcgca aggttgtgag aataaaaacg ttattattat tggtgccgga
    24301 accattggcc tgctggctat tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggcg
    24361 atcgacatta gctcagaaaa actggcactg gcaaaatctt tcggtgcgat gcaaacattt
    24421 aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt tacgcgaact gcgctttaat
    24481 cagcttatcc tcgagacggc tggcgtaccg caaactgtcg aactggcggt agagattgcc
    24541 ggtcctcatg cccaactggc gctggtgggc acgttgcatc aggatctgca tttaacatcg
    24601 gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta tcggcagttg gatgaactat
    24661 tccagccctt ggccggggca ggagtgggaa acggcgagcc ggttgctgac agaacgtaag
    24721 ttaagcctgg agccattaat cgctcaccgt ggaagctttg aaagcttcac ccaggcggtg
    24781 cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc tcattccatg aaaccgcggg
    24841 ccagcgtgat gctggcccgg tattgtgcaa aacagatcat tcaccaatgg tcccccttcg
    24901 tttacactag ccacaattga atatgggtaa atgacgatga attcattcga gcgaaggaat
    24961 aagatcatcc aattagtgaa tgaacaggga accgtgcttg ttcaggatct ggcgggagta
    25021 tttgctgcct cggaagcgac aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg
    25081 gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg gtaatagtga aaccgagacc
    25141 caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg cgccaaaaaa cagaatagcg
    25201 caggcggcag tcaaaatgat ccatgaaggg atgaccgtta ttctcgacag cggaagtacc
    25261 acaatgctta tcgccgaagg attaatgacc gctaaaaata tcacggtgat taccaacagt
    25321 ctcccggcag cgtttgccct ttccgaaaat aaagacatta ctctggtcgt ctgtggtggc
    25381 acggtccgcc ataaaacgcg ctcgatgcat ggttccattg ccgagcgttc attgcaggat
    25441 attaatgccg atttaatgtt tgtcggtgct gatggtattg atgcggttaa tggtattacg
    25501 acctttaatg aaggttattc gattagcggg gcgatggtca ccgccgcaaa taaagtgatt
    25561 gctgttctcg attcatcgaa atttaatcgt cgcggtttta atcaggtact gccaatcgaa
    25621 aaaattgaca ttattgttac cgatgatgct gtgtcagagg tggataaatt ggcattgcag
    25681 aagacacggg taaaattaat tacggtgtag tattgttctg tatgccggat aaggtgttta
    25741 caccgcatcc ggcattacgg attctccctg gttcaagaga gaatagtttg attaacgcaa
    25801 taatggacaa tctggtggta atcgacaacg caacgccgcc ggaagtattt caatatggaa
    25861 gttttgccca ctcaacggtt cgccatcaag attaaaagtg atttcgtgtg gggcttgtat
    25921 atcaaaccac gacgaagcgc cttcgataat attcgggtta tcttcgtcag attttaaggt
    25981 tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc
    26041 gttaattaac gcgttcgggc acaattgttg accgccaccg gcctgacgcc cgttaccaat
    26101 accaatgacc agggcgtcac cttgccagtg aaagttttca ccgcggattt cacaacggtc
    26161 cggttgcaga gtatccatgc gcattaagcc atgaatgatg taagagacgc cacccagcgc
    26221 ggcttttaat ttttccggcg tttctgtggt aatacgcgtc ccaaatccgc ctgtcgccat
    26281 attaataaaa caggtttgtt tgttgacctg cgccatatct atcgcaatgg cattaccggc
    26341 aattgccagt ttcagcgcct tatccagtgc ctcaggaatc cctacactgg tggcaaaatc
    26401 attggcggtt cctaatggca aaattcccag cgcgggtatg tcatccccct cacactgaat
    26461 caacgccgta gaaacttcat taatggtgcc atcgccacca ccggcaatca ccgttgcgac
    26521 gcccaacttc cgggcctcct ctacaaatcg tgcggcatca cctttctccc aggtgacccg
    26581 cacatggatc gtcattcctt cctcacgcaa cagcataatt gcttcgcgca agggtagatt
    26641 gtcagtactt ttaccattaa gaatcagtaa gctggcggga aattctgcca tgatcgtatg
    26701 tgcctttatg attggtctgt agatagtgta gagcagaaaa caaaaggtgg agtcagaaca
    26761 ggatgaaagt cggaggataa aaagtcagtt caagtatgga ggattcagtg gttaccacca
    26821 ggagctttca atggggacag cgaatactaa cggctgccac cgataaattt caaaaaagag
    26881 catataccta atattcaact aaacagtggc atcttcaata taatatatta aagcccccat
    26941 ggagttaccc tgaagggcct caatgtccgt aattcctact tatgtaggaa atgttgtaca
    27001 gagcatttat tataatccta ttcaattata ataatcatgt cattattata tttaaacact
    27061 agagagtgtc gttggtattt aatgggggaa ggtgagatga aaaagatagc tgctatatca
    27121 ttaattagta tttttcttat atctggttgt gctgtgcata atgatgaaac aagtatcggt
    27181 aaatttggtc ttgcatataa aagtaatatt cagcgtaaac tcgataacca atactacacc
    27241 gaagccgaag cttctttagc caggggcaga atatctggtg cagaaaatat agtaaaaaat
    27301 gatgcagccc atttctgtgt tactcagggc aaaaaaatgc agatagttga cctgaagaca
    27361 gaaggtgcag gattacatgg cgtcgctcgt ctgacattca aatgtggaga gtgagaatat
    27421 ttttttgtaa gcgtcaaaca tgcgcgttct ggctgtgcgt agccggaacc tgggcgagca
    27481 cgataccgtt acgtgaaagg cattgtgcta tgaaggggga ttctatcgat gtggtcaatg
    27541 gaagacggtt atcagggatc aatccttctt tacagcttat gtacaaaaaa taagcccgtg
    27601 taagggagat tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatggatta
    27661 tgtttttcag cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta
    27721 agaattttcc atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgtg
    27781 gtttccccgg agaaattacg cattagcagc gcgtaattta gctcaagatc ctgcgggacc
    27841 gggagccaca cagtataacc atcgcctggt gcgaccggca tagcttcgcc tttggcgttt
    27901 tccatgtgct caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aagactgtcg
    27961 ccaacggaga atttattttt caccgctacc gctgcgaggt cccccttgcg ctcaccggta
    28021 aactcaccaa caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa
    28081 tcgtcgtgag tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc
    28141 agagtttcca gcaggctggt atcgaacggt tttcccgcag cggcgtcatc gatagctttg
    28201 cggtaaacct gtgcggtgcg tgcgcaatag tagaaagatt tggtacgacc ttcgattttc
    28261 agcgaatgca cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc
    28321 gagttcatga tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc
    28381 tgggcttctt cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca
    28441 acattttgca ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc
    28501 ccttcctgga cgttgtactc ccagcggcag gcgttggtgc aggtgccctg gttcgggtcg
    28561 cgcttgttga tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg
    28621 tgaacgaaga tctcgatctc catatccggc acctgattat ggatctcttc aatctcttcc
    28681 agcgacagct cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc
    28741 gtcgcccagt tcacggcgtt agcctgcacc gagaggtgga tcggcatttc agggaagtgc
    28801 tcacgcacca gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc
    28861 accaccggtt tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg
    28921 accacgacat aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaagattt
    28981 tcgtggttga attcgttgtt gcgcacacgt agggagtaac gcggctggcc cgcataaaca
    29041 gcatctgcgc cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg
    29101 agttccggtt taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat
    29161 gaagcggtta ggggaggccc ccactttaag ggcgggcatt gtagcgctgt aggggggatc
    29221 tggtaaagcg ccggacgcca acaacccacg cccggcatcg taattacgta ttgtctttca
    29281 ccgtctggct tgtgccattg gctttaacgg aggcaattcc ggtttcacga gattgcgcgg
    29341 tggcgtacat ctggctggag ccgataattt gatgattagc ggctttcaga ttgaaataga
    29401 atttgccgtt gcttgccgtt ttttctcata gcgctcttct tgcgggctgt tgctacgcac
    29461 cgacgcgatg cccttttccg cagaggcttt tgaggtatac agctcgctgg tgaggatagt
    29521 ctcaccattg cccgctttta gcacaaaccg gaactgatta tcactgctct tactgagttc
    29581 aaaccaacca gccataatga ctccttgatt gaaattgctt atcgaaaaca gcatctaa
//
LOCUS       DAJTNW010000049        29408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNW010000049 DAJTNW010000000
VERSION     DAJTNW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..163
                     /locus_tag="OO179_002234"
                     /pseudo
     CDS             1..163
                     /locus_tag="OO179_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            836..2344
                     /gene="ltrA"
                     /locus_tag="OO179_002235"
     CDS             836..2344
                     /gene="ltrA"
                     /locus_tag="OO179_002235"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5532279.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2425..2912
                     /locus_tag="OO179_002236"
                     /pseudo
     CDS             2425..2912
                     /locus_tag="OO179_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2995..3252
                     /locus_tag="OO179_002237"
     CDS             2995..3252
                     /locus_tag="OO179_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532280.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3810..4577)
                     /gene="fecE"
                     /locus_tag="OO179_002238"
     CDS             complement(3810..4577)
                     /gene="fecE"
                     /locus_tag="OO179_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5532281.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4578..5534)
                     /gene="fecD"
                     /locus_tag="OO179_002239"
     CDS             complement(4578..5534)
                     /gene="fecD"
                     /locus_tag="OO179_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5532282.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5531..6529)
                     /gene="fecC"
                     /locus_tag="OO179_002240"
     CDS             complement(5531..6529)
                     /gene="fecC"
                     /locus_tag="OO179_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5532283.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6526..7428)
                     /gene="fecB"
                     /locus_tag="OO179_002241"
     CDS             complement(6526..7428)
                     /gene="fecB"
                     /locus_tag="OO179_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5532284.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7473..9797)
                     /gene="fecA"
                     /locus_tag="OO179_002242"
     CDS             complement(7473..9797)
                     /gene="fecA"
                     /locus_tag="OO179_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5532285.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9884..10837)
                     /gene="fecR"
                     /locus_tag="OO179_002243"
     CDS             complement(9884..10837)
                     /gene="fecR"
                     /locus_tag="OO179_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5532286.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10834..11355)
                     /gene="fecI"
                     /locus_tag="OO179_002244"
     CDS             complement(10834..11355)
                     /gene="fecI"
                     /locus_tag="OO179_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5532287.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11653..11835
                     /locus_tag="OO179_002245"
                     /pseudo
     CDS             11653..11835
                     /locus_tag="OO179_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11829..11933
                     /locus_tag="OO179_002246"
                     /pseudo
     CDS             11829..11933
                     /locus_tag="OO179_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12273..12530
                     /locus_tag="OO179_002247"
     CDS             12273..12530
                     /locus_tag="OO179_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5532288.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(12850..12981)
                     /locus_tag="OO179_002248"
                     /pseudo
     CDS             complement(12850..12981)
                     /locus_tag="OO179_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13263..14621
                     /locus_tag="OO179_002249"
     CDS             13263..14621
                     /locus_tag="OO179_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5532289.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(14860..16245)
                     /locus_tag="OO179_002250"
     CDS             complement(14860..16245)
                     /locus_tag="OO179_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5532290.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(16295..16642)
                     /gene="tnpB"
                     /locus_tag="OO179_002251"
     CDS             complement(16295..16642)
                     /gene="tnpB"
                     /locus_tag="OO179_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5532291.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(16639..17019)
                     /locus_tag="OO179_002252"
     CDS             complement(16639..17019)
                     /locus_tag="OO179_002252"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5532292.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(17374..17808)
                     /locus_tag="OO179_002253"
     CDS             complement(17374..17808)
                     /locus_tag="OO179_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5532293.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(17796..18197)
                     /locus_tag="OO179_002254"
     CDS             complement(17796..18197)
                     /locus_tag="OO179_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532294.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(18457..19026)
                     /locus_tag="OO179_002255"
     CDS             complement(18457..19026)
                     /locus_tag="OO179_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5532295.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(19771..19914)
                     /locus_tag="OO179_002256"
                     /pseudo
     CDS             complement(19771..19914)
                     /locus_tag="OO179_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(20248..20514)
                     /locus_tag="OO179_002257"
     CDS             complement(20248..20514)
                     /locus_tag="OO179_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532296.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            20655..20861
                     /locus_tag="OO179_002258"
     CDS             20655..20861
                     /locus_tag="OO179_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5532297.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            20956..21558
                     /locus_tag="OO179_002259"
     CDS             20956..21558
                     /locus_tag="OO179_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532298.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            22758..23675
                     /locus_tag="OO179_002260"
     CDS             22758..23675
                     /locus_tag="OO179_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532299.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            23760..24632
                     /locus_tag="OO179_002261"
     CDS             23760..24632
                     /locus_tag="OO179_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5532300.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            24850..27234
                     /locus_tag="OO179_002262"
     CDS             24850..27234
                     /locus_tag="OO179_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5532301.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            27231..28136
                     /locus_tag="OO179_002263"
     CDS             27231..28136
                     /locus_tag="OO179_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5532302.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            28133..29203
                     /locus_tag="OO179_002264"
     CDS             28133..29203
                     /locus_tag="OO179_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5532303.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         7067 a         7627 c         7922 g         6792 t
ORIGIN      
        1 gcttgctggc agactcatga aagagctggg gctggtcagt tgccagcagc cgactcaccg
       61 gtataagcgt ggcggtcatg agcacgttgc tatcccgaat catcttgagc gacagttcgc
      121 cgtaacggaa ccaaatcagg tgtggtgcgg tgatgtgacc tatagtgtgc ccggagttca
      181 gggcgggcat ggatgcttaa atgaaccgcg agtctgtctg gaatattgaa ccggtaactc
      241 acgatgagaa acccaacaat cccaccgggt gtgacggtgg agaacctgag cggcagtgac
      301 ctgcggcatg cccgcagggt gatgtaaccc gctgacaacg gggattgagg cgagatcact
      361 aagccgagat gatcctcaag gttaagtact gaaaggctga agaacatgaa cccgttaatc
      421 cgcctctgtg ggttgaaaac gtcaccacgg cctacgtgat ctgacaggcc gtgcaggagg
      481 aactggcagt gatacgtaag cactgccggt cgaaggtgtt ttgacatgta tgcgaaacac
      541 cggggcagca gcgtctatca cgcttgcgtt gctgacttct gccaacttgc ggcaagcaag
      601 gataaagagt gcgacgggca gcctcctcag tatgcctgag tccaggcagg taaaccgggg
      661 aaggtcagcg acggatgtta agggggcatg gctccgatga cgcgctgact ggcggagctt
      721 ccgtagtagt ccgcgatggg gaaagcccat tacatggcga agggaagcag tttgaatgtg
      781 tttgcgacgt gaattaactg gcctaacgag gtgaagacct ttgataatca gcgaaatgca
      841 acgcaagctt gccacatggg cagccaccga tccgtcccta cggattcaac ggctgctgcg
      901 tctgataaca caaccagaat ggctggctga agcggcgcgg atcacgcttt catcaaaggg
      961 ggcgcatacc cccggcgttg atggcgtgaa caaaacaatg ctacaggcca gactggctgt
     1021 tgagctgcaa atcctcaggg atgaattact ctcaggccac taccagccct tgcccgccag
     1081 acgggtttac atccctaaaa gcaacggcaa actgcgacca ctgggtatcc ccgcgttgcg
     1141 ggatcgtatt gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt
     1201 tcatacgctc tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt
     1261 gaaattacag ctcacagact gcggtgaaac ccggggacgc tgggtgattg aaggcgacct
     1321 gtccagttac ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat
     1381 cagtgacgca cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt
     1441 cggtctcttt cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt
     1501 atcgaacatc atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa
     1561 agccagaaaa gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt
     1621 cagagaaaac tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt
     1681 cctcatcgtc aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt
     1741 gctcgaaggc agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa
     1801 tgacggcttt atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat
     1861 gcgagtggtc tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc
     1921 actgttatca ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa
     1981 actgaaaggc tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta
     2041 tatcgaccgt gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg
     2101 tatcgcttcc ctgatgaggt ggtggtgtaa atcaccggaa ccgggtcaga gcaaaacgtg
     2161 ggttttattt ggtaaaacca atcacggcaa gctcagcggc gaaatactgt accggttggt
     2221 ggggcaaggc aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac
     2281 ggagaccaga aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat
     2341 ttaaatggag agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg
     2401 aaatagtccg actacgccct gcctcttact ctactctgga cgggtaagcg ctgggcgtac
     2461 ctcgccgttg ttctcgacct gttcgcaaga aaaccagtgg gctgggccat ctcgttctcg
     2521 ccggacagca ggcttaccat gaaagcactg gaaatggcat gggaaacccg tggtaagccc
     2581 gtcggggtga tgttccaagc gatcaaggca gtcattatac gagcaggcag ttccggcagt
     2641 tactgtggcg ataccagatc agacagagta tgagccggcg tggaaactgc tgggataaca
     2701 gcccaatgga gcgcttcttc aggagtctga agaactaatg ggtgccagcg acgggctatg
     2761 taagcttcag cgatgcagct cacgcaataa cggactatat cgttggatat tacagcgcac
     2821 taagaccgca cgaatataat ggtgggttac caccaaacga atcagaaaac cgatactgga
     2881 aaaactctaa cgcggaggcc agttttagtt gaccacaaca gactacctga agggagccgc
     2941 ggtcgcctag cagttgcagt agcaggagat catccagccg cagtacaggt cacgatgact
     3001 ctggttaatg ataccggctt tgaccccgta ttttccggct ctatcgctga atcatggcgt
     3061 cagcagccgt gcacaccatc ctattgttgt gactgggagg ctgccaccat gcttcgcgct
     3121 ttccctctgg cgaaaaaggg agaaggacgg gcccgtctgc cttcacttta tgccagcttc
     3181 ggtaagctgg gtgagacacc gactcatgaa gatatcattg ataacaatcg atccatcaac
     3241 tggcctgtat aacgtggctg ccggtgatta agaaagctgc acctacctaa gtagtagcaa
     3301 acgcacactt tttagaaaaa tcgatggtca gaaactggat tagcaattcc gttccagggt
     3361 tgcttttgat ttacgttggc gtctgatcat tgatttatcc tcaaaagccc aacctcattg
     3421 gtaatgaacc agctccgtga atgtccgctc tggcacagag cgaaattttt tgatctcccc
     3481 ccctgaaatc taaacttagt catgtcacgt ttttgggttt ctaaaatttt aacttcgcgt
     3541 ttttcgttgc cgtaagggtt atacagaaat gtccgttaag cagagttcaa aattgattgc
     3601 cgtgatcacg actggtttga aagccgcgcc caagcctgta cagctctggt ttgcgttgat
     3661 tatgaacctg tcagcctaaa gcaagcggat ggacgatgag tattggtaat ctttcagagt
     3721 ccggaaaagt tcagccccag tctgaacagg cttgctggcg ccagtccagt ttcattcagt
     3781 cgtggtttgg ttcttacggc ctgtgcaatc tacctcatta ggcacatcgg cctgccagat
     3841 accggctcgg ggtgtatttc cgcttccacg ctgaatactg ttctcagcaa tcctggggtc
     3901 atcacctctt ctggtgtgcc ttgcgccata acatgtccgt ttgccattac caccagttga
     3961 tcgcagtacc ggctagcctg attaaggtcg tgcagcacag cgaccaccgt tttcccctga
     4021 gtccggagtt cgcccatcaa ccgcatcagg tccacctggt gattgatatc aagataggtg
     4081 gttggctcat caagtaatac aacgggcgta ttctgggcca ggaccatcgc cagaaatgcg
     4141 cgctggcgct gaccgccgga aagctcggtt aaccgacgaa cggcaagatg attgatccgg
     4201 gtctggttca tggcgacatt aactcgtgca ttgtcttcag cggagagacg cccccagagt
     4261 gacagccagg gattacgacc atacgaaacc agctcctgga ctgtgatccc ctctggcgtt
     4321 aaatggtgct gaggcagcag cgaaagcctg cgggccaact ggcgtgatga gagcatattt
     4381 atgggattat cgccgagaaa tacggtgcca gactgcggca ttaaaagccg cgaaaaacag
     4441 tttaacagcg tcgatttccc gcaaccgtta gggccgatca gggcggtgat cttccccgtt
     4501 ggcagtgaga gtgaaacgtc gttaagtacc ttgtctgtcc cgtaactgac cgtcagattt
     4561 tcagttcgta aagtcattta tcgcattctc acaagcaacc agacaaacca cggcgcaccg
     4621 ataatggcgg tcagcacgcc aaccgggagc tccagtgggg gatgaataat tctcgccagc
     4681 agatcggcaa ccaccaacag caacgcacct gtcagggctg aaacaggcag cagtctgcgg
     4741 tgacgtccac cggtgatgct acgcatcata tgcggcacca cgagaccaat aaagctaatc
     4801 gggccgcagg cggccacgcc ggtagatgtc atggcgacag ctagtaacaa agcccagaat
     4861 cgggtatggg gcaccgacac accgagcgtg gtggcgcgcg catcgccgag tgcaaggagg
     4921 tcgagatcgc ggcaaaaact caggctcagc ggcagaaata aaatcatcag cgggatggca
     4981 atcttcacaa agctccagtc acggccccat aagctgccgg tcagccacag cagggcgttg
     5041 ttcacatcct gcgggcgcga gagcatcaga taatccgtca ggctggccca gcatgcagaa
     5101 agcgccacgc cggtgagcgc cagcttcatc ggctggtggg tctttgccag catcttcagt
     5161 aatatcaacc ccgccatgcc gcccgcaaag gccagcagcg gcagcaccat cacgggcagt
     5221 gacggcataa gaagtagagc ccccacagag gccaggctgg cggcatggtt aacgccgaga
     5281 atatccggtg atgccagagg gttgcgcaca atcccctgta tcagcacgcc cgccacggcg
     5341 agggctgcac cgacaaacag tgccagcagc aagcgcggca gtcggtactc catcaataca
     5401 taataatgct cgcgtccggc ctgccagtcg gtcagcagcg cgcgccacgg cacggggatc
     5461 actcccatat ggagtgataa cagcgcacag cccgccaggg caagggtgat gaaaataacc
     5521 agcgcaattt tcatcctcgc ctcctcacaa gccagacaaa gcaagggctg ccaatcagcg
     5581 ccagcactgc gcctgcgggc agatctccgg ggaaggccag cgcgcgtgcg agtacatctg
     5641 ccagcagcat cagcgtggcc cccagcagca tgctcactgg cagtacgttg cgctgatcga
     5701 agcctgccca gaagcgcgcc agatgtggca ccagcagacc gataaacgcc accggacctg
     5761 ccacgctgac gcacgcacca accagaagca gcactaacat attgatgacc aaacgtagcc
     5821 tcgtcaggtt cactcccagc gtatgggcgg tgctgtcgct gaggttgagc aggttcagtt
     5881 gattcgccag cagtaacacg acagggactg cagtgaccac caccggcaag agctgccaga
     5941 catcctgcca gcgggcgtgg gacactccgc ctgccagcca gtaaaagatg ccgtaagcat
     6001 gatcttcggc cagcagcagg gtgatgcggg taaggcccat acaaaaggcc gacagcgcga
     6061 tacccgcgag gatcagtttg tttctgtcat gggtatgacg aaatccgcct cctgcggtca
     6121 tgaccagcag ccagctcacg ccgcccccgc atgccgcgat gaacgacaga gaatagcctg
     6181 caatcggcgt cggactcagc gcgctggtaa gcgccatagc cagcgccgcg ccgctgttaa
     6241 tgccgagcag tgaaggagag gccattgggt tgtgggtcag ggtttgcagc agcgtgcccg
     6301 cgagcgccag gcttgcgccg atcagaacgg cgaccaggct tcgtggcaaa cgaaggtttt
     6361 gcaccagcgc ttctggtagc gttggcgtgt gtccaggcag cagggcgcgg gttgcatctg
     6421 ctccggaaac aggaatggcc gagtagcaaa acagactcag ccagaaaata ataataagtg
     6481 ctgcaacggg aagcccccac agcagcatcg ggtgttttat cgcggtcatt tcacaacggt
     6541 aagcggctga tggtggaaga tttttaccgt gtcagcggca atacgctctg cagcaaaaat
     6601 accgcgcatc cgcgcccagg tgttactgtc gaccgaagca acctgctgct tctgcgcggc
     6661 ggttaacatc tgccagagcg gatcttgttg ccagcgttta acaatgctct cttcgcgata
     6721 gtgggcaacc agcagccagg caggattgac cgccagcagt tgctccaggc cgatggacgg
     6781 catggacgaa cccgccatcg cagcgggaac gttcagcccc agagaggcca gcacgctgcc
     6841 ggtccaggtc tcctgagtat gcaggttgaa ttgctgttcg cgtgatgtgc caaaggccac
     6901 gcgtgtccct ttgggaagct ggctggccca ctgcgccatc ctctctttat gttgttccag
     6961 acgtgcctgc atctctcgct ttttacccac catttcgccg atgatagccg cagattgcaa
     7021 attttcagcg taggtttcgt tgcgggactt aagcagcagt accggcgcga tttgctgcaa
     7081 ggcgatgtaa acccccgcat ggcgactgct gtcggcaatg atcaggtctg gtttcagagc
     7141 ggcaatggct tccaggctcg gctgcgcgcg cgttccgacg gactgccacg gtttcaggtg
     7201 cgcacgcact tcgggcagga tgcgttttgc atcgttatcg tcggcaatac cgatcgggct
     7261 gacgtccacg gcggccagcg catcggcgaa cgagagttcc agcaccacaa tccgttgtgg
     7321 cgttttttcg agtgtaaacg tgccgtgttc gtcctgaacc atggcggcaa aggcgtggct
     7381 gatcaccagc agcaggcctg caaaaagaaa acggataaat gccaacataa tcacattcca
     7441 gctaaaagcc cggcaagccg ggcgttaaca catcagaact tcaacgaccc ctgcatatac
     7501 agcgtgcgcg gctggcctgc atagatgcct ttgttgttgt cgtcataaga gcggatgaag
     7561 tagtcctggt cgaagatgtt tttcacaccg aatgccaggt tcagatctgc catctgcggg
     7621 ccaaagtcat acgccacgcg tgcaccccag agcatgaagc cgggaatgcg gccggtactg
     7681 ccgtcggcgc tctctttcac cgtattggcg ttatccgcaa actggctgga ctggaaatcg
     7741 ctgttcagat tgaacgtcca gtttcctggc ttgtagtcca cgcccagcgt gcctttatgt
     7801 ttcggggaga atggtaccag attgccgtag gtgtcgcctt tctcgcggat ttccgcgttc
     7861 acatacgcat agctggcgta gatggaaacg ttatcaagcg ttggcgttag cgtacccaga
     7921 tcgtaacgtg cctgcgtttc cagcccggta tggcgcgttt tgccacgtgc agtgacggtg
     7981 tcgttggtct ggttggagtc gtactgattg ttaaagttaa tcaggaacag ccccatttcc
     8041 gccgtcagcg cgccgtcgtc gtagcgggta ccgagttccc aggttcgcgc tttttccggt
     8101 tccacattgc cgctttgcac agccttgcca atctggctgt actgtacggt gccgaacgag
     8161 ccttcagtgt ttgcataaag attccagctg tcagtcaggt gatagagcac gttcaacgcc
     8221 ggaagcggtg cgttatagct cacttcttcg tgcgtgcctg tgatggcgtt gttctggtat
     8281 gactcgatat gttcgaaacg catacccggc gtgatggtcc agttgccgat gtcgattttg
     8341 tcatccagat accaggcgtg cgcctcggtg ccggaacgcg tatcgcggtc gtaagggctt
     8401 gagccggacg gcaactgccc gctgctggtg gcggtgtagt aacgcatttc atgcgttgat
     8461 tcattcacat agcgatagcc cacgcccact tcgtgcgcgg aagggccgat cataaagatc
     8521 tggctgtagc gtggctcaat accgcgcacc cagtagttac gcggcgagag ggtgatgcgt
     8581 ttgccttgct ccaggtagcc gctgcgcagg gtttgggtgt agaacccctg aatgttgaat
     8641 ttatgctggc tgtctggctg gaactggtag cccaggctcg ccagcttgcg acgaccccag
     8701 aagcggtcat acgggcgggt ggattgccag cgatcggcgt cgtaatccgc gcgagacagg
     8761 ccaccgggca tgtcggcttc accgtcgtaa tattgcagca ggctgttgaa ggtgtgcacc
     8821 tcatccggcg catatttgct tttcagcatc aggtcgtcga tgcgggtggc gctgtgctcg
     8881 cgccagtcac tgccgcgcgt gccggagtag agcagcgcgg tgccaaaacc gttgtccgct
     8941 gtgccgccca ccatcaggtt gtgcgtctct ttcgggttgt tttgtgaaga ggttgggctg
     9001 agctgacctt ccacgcccgc ctcgatacca aagtcctgcg gaatggcacg ggtaacaaag
     9061 ttcaccacgc cgcccacgct ctgcggtccg taacgcaccg caccaccacc gcgtaccacg
     9121 tcaatggcat ccatgttgcc gagcgaaatg ggagccagtg aaagctgcgg ctgaccgtaa
     9181 ggggcgaagg ggacggggat gccgtccatc aggacggtcg agcggctggc gaggcgcggg
     9241 ttcaggcccc ggatgccaaa gttcatcgcc aggtcgtggc tgccggtgcc gttgttttcc
     9301 ggcgcgctga cgccagggat gcggttaagt acctcacgca tggtggttgc gccggttttg
     9361 gcgaaatcct cacggcggat cacgtcacgc gcgccagcat gttcaaatac gtcgttttca
     9421 cgcgcatcac ccagccagtc gccgaccacg gtcagggcat cttcttttgg tgcgggcgcg
     9481 ggctccagcg tccagctgtt atttcccagc ggttttacct gcagtccgct gccgtccagc
     9541 agttgttgca ggccgctctc gacgtcgtaa tcgccgtgca ggccgttgct ctgcttgccg
     9601 cgcgtcaggc tggcgtcaac cgagagggta aatccgctgt gtgcggcata ctgattgagc
     9661 gctttatcga gcgatcccgg tgcgatatta acctgtgcag caaaagcgga aaacgagaga
     9721 ccggccagcg gcagcaggct caggcgaatg gcgttaacca aaggtgttgt tttacgaaaa
     9781 acgcgtaacg gcgtcatacc ttccccatca tcatttttgt tgtgttcagc tatgagtcga
     9841 acgagaaggg taaaaaggac aatcgaaata agaattattt tccttacagt ggtgaaatgt
     9901 ttatccagta ccgcgtaata gactgaattt taacgggaag cgtttgcgcg ataacgttca
     9961 ggatcgcatc ggtatttttc agcgggaacg tcccgctcag gcgcagcccg gcaacggcgg
    10021 gatcgcagcg cagcacgccg ttgcggtaac gggttagcgt ggctatcacc tcacccagcg
    10081 gtttatcgct gaagctcagg atgtccttcg tccagcttgt actctcgtca tccagcggtt
    10141 tcactgcgcc aaactcagag gcgctgaact gcaggctttc accagcgttc acgatgcgtt
    10201 tttgcgcggg ggcactggcg agaagcactt ccacagcgtg ctgctgaacg tcaagctgcg
    10261 tgaaattatc ctgctggcgg acggtaaatt ctgtccctaa agcagtgagc tggccctgac
    10321 gggtcaggac acggaagggg cgttgcaggg catctttcgc ggtagtaatg gcgatttcac
    10381 cgtaccagag ccggacggtg cgctgatgcg catcaaaacg cacatccgcc gcgctttggg
    10441 tattcagcgt gagcagggag ccatcttcca gttgctgacg gctgactgtg cctttggcgg
    10501 tgcggtaatc tgcccgcaga ccttcgccgg tttccgactg ccagagctgc cagcctccgc
    10561 cagcgccgag caacagcagt aatcctttca tcacgtgacg gcgggtgagg cgggtatcgt
    10621 gcaacgcccg gctggcaacg tcgccaggca caccgccaag ctggttgcgc aggttttcaa
    10681 cctgctgcca ggcccactgg ttatcctgat cctgttcata ccactgttgc cagcgcgctt
    10741 cctgttgtgg gctgacgcgc tcgccgctta gcacggcata ccagtgggaa gctgaacgca
    10801 gcgcctgacg gcgggaatcg gttaacaaag gattcataac ccatactcca gacggaacag
    10861 caggcagtgc tcgacggctt tcgccacgta ttttttcacg gagctgatgg aaacaccgag
    10921 tttgtgcgca atctcgctgt atgtcagacc atccagttgc gaaagcagaa acgcttcacg
    10981 tgttttgccg tttagcccgt ccagcatgct gtcgaggagt tgtagggtct cgagttggct
    11041 ttcgcgttcc tcaggtgaag gcgctccccc ctccggcata agcgccagca tctccagata
    11101 cgctttttcc agcgcgtttc ggcgaaacag gtccaccatc acgcgtttgg cgatagtgca
    11161 gaggaaggag cgaggatcgc ggatcgtcga gagcgtttcg ctgaccatta cccgcaaaaa
    11221 agtgtcctgg gcaatgtcat ctgcatcaaa agcagactgg agtttgcgcg tcagccagct
    11281 tttcaaccag ccgtgatgtg tgccataaag cgactcgaac gttaaggaag ctgtggtagt
    11341 ggcgcggtca gacatgcgga gtgcatcaaa agttaattat cacgtagtca tattaatatg
    11401 agaatggtta tcattacaat tggaaataaa attgtttcca atagacattt ttaacatgtt
    11461 gtttttctaa gtgttataag gtaggtataa aatgggatgg agcctctgct tctgacatgt
    11521 gtcggtcaga atgactcatg atgtggtctg ctattattga catcctcact gccctaaagg
    11581 atggggattt cggtaatgat cttacccagc aatagtggac acgcggctaa gtgagtaaac
    11641 tctcagtcag aggtgactca caagacaaaa cagtttcaac cagtaaaaaa acgtaaacac
    11701 cattcgcatg aatttcgtag ttaagatctg aagcttgcag aatgcatcgg tgttactgcc
    11761 ggagtccgtg aacttagtct gcatgaatca ccacagcttt acatctgtcg cagtaaacag
    11821 caaaatcagc aaacttactt aattatatcg agtgcgatta caatcgctgg cgatggtaca
    11881 gttggtgtgg cggtctcagt ccggaacaat ttgaaaatca taatctcact taaaacggca
    11941 tccattttac aaaagatggc cgtctgtgta tttgtaatga ataccttcta catgtttaga
    12001 gtgcataatc cacgacaagg cgatatccta aaaattagct agcaggcaac tttgatggca
    12061 tagataatgt ttttagtaaa aaagagttga ctgaatataa tacagcctct tcctacataa
    12121 gaaggaatgt gctcacaaaa aaaaaccatt agtctgcttc ctgttcttac aataatcagt
    12181 attatgtatt ttatttatat agcaaaatat gcttgttaca cctgtcataa ttaggcatgg
    12241 taaaattatt gactgaaccc tgggggagaa agatgcacag tgattattat aatatggttt
    12301 ttggtgaaaa attggccaat attttatatg aagctaactc tcaatttttt tacgagagaa
    12361 atgtcataga agaggcagtc aacgctttat tctgtgagcg tgaaataata aacaacaaga
    12421 acataataaa gaagctgatg ttctttttgt cagatgttaa tcacaccaag aaagatgttg
    12481 ttcagagcgc acttaatatt atcattgaca ttacatcagg tgatatttga gattttacat
    12541 aaaagataat ctctcaagga gtcttatctg aaaacgaaat atatagtccc tctcgttttg
    12601 taatatcgat tggctttgtt gaatcggtta cgtaccccgg agccggtcat attttcaggt
    12661 aatacacacg ctttcagaca tttcggcttt cgtcattttt gtatcatggg catagcttct
    12721 gctacctgac catcataatc acgcagcttc agtgaagcag tgaccacctg tttaacccgg
    12781 tgtatcgtta tgatcttatc cagcaattga accgccccgg gtttcctgga gagtgtttta
    12841 tctgtgaact caggctgcca gatcatcgtt tccgatggaa gcataataag ctttttctgc
    12901 ttctgccggt gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac
    12961 ccacgtgagt gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc
    13021 aaccgtacaa tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca
    13081 cagcattttt gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa
    13141 taacaacagt gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca
    13201 ttttcctgac tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca
    13261 atatgaaaag aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg
    13321 cggcacaacc tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact
    13381 acgccggcgc atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc
    13441 tgctgttcac cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc
    13501 cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt
    13561 tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc
    13621 gtcctctgca tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg
    13681 gctccaccaa tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg
    13741 ggctggatac ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt
    13801 atggcccgtt cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc
    13861 aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc
    13921 aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg
    13981 tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg
    14041 tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg
    14101 cctacagcaa aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca
    14161 tcctgctgat cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg
    14221 tcgatctcaa taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt
    14281 ttgatgatga gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg
    14341 ttgatctgcg ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca
    14401 gcaaaacgct ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc
    14461 cggtcgaggg caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg
    14521 acgacaagca ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca
    14581 gtgaactgtg gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag
    14641 cagtatcgct tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac
    14701 ccccaatgaa agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg
    14761 tggcagccgg ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa
    14821 aagtaagcgt acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat
    14881 gcgccacgga agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac
    14941 atggcgaagg tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta
    15001 cagtagcgct ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc
    15061 acgcaaccag ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag
    15121 ccgctgctct gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg
    15181 ctccagggct tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg
    15241 gcgggcatga gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa
    15301 cccggcgtac gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg
    15361 gatgcttttt ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc
    15421 attgcggtca tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg
    15481 cagcagtacc tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc
    15541 atgaagcgcc tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag
    15601 cagtgaacgg ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg
    15661 ggtgtgctct gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc
    15721 ccgctcgatg ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc
    15781 atgtttttcc cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc
    15841 ggcggtatcc tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc
    15901 aggcaacagt cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt
    15961 ctgacgcaac ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc
    16021 tttctgaagc cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga
    16081 acgactgccg aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc
    16141 gcgctcccgg ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag
    16201 gcgctgtttc agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac
    16261 cgggcttatt ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca
    16321 gccgcttggg ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa
    16381 ggaacacttt gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg
    16441 tcaggaggca cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc
    16501 cccggaaaat gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg
    16561 cagccaggcc gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg
    16621 tcccggaagg taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc
    16681 gctgacattg ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact
    16741 gagggtttcc gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac
    16801 aggaagtagc tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg
    16861 tcgccatttg aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga
    16921 tatcccgggt tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg
    16981 gcagcctttt cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac
    17041 tacttatttg gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc
    17101 tgtacgctta ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg
    17161 gaaacgcaca cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac
    17221 gaatggaatg acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa
    17281 aggccaccaa caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa
    17341 aggcatcacc gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc
    17401 cctggcatct tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc
    17461 gcagcgtggg cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt
    17521 tctcaccttc accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc
    17581 tttctctgcc gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg
    17641 gtcatgttca aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc
    17701 acaggacaca cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac
    17761 aaaatggcct ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga
    17821 gttgtccggg gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa
    17881 taatcgtggt gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc
    17941 ggtacgaatt gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa
    18001 gggcactgtt gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca
    18061 gtttcgtatt gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca
    18121 taaggtgcgg cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata
    18181 aaggattttg cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg
    18241 tcccggtacg ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga
    18301 gtttgggtaa agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact
    18361 gacgtaaaaa acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata
    18421 cttttggttt gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt
    18481 gcgctcaccg gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag
    18541 cacttgctga aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa
    18601 atgcaccagc gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt
    18661 aatcatcccg gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg
    18721 gttgaccagc agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc
    18781 ccagacgtaa tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg
    18841 gtaagcgtcg attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt
    18901 tgctgccggt acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac
    18961 attcagtgcg tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta
    19021 ggtcatggtt aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag
    19081 agtccctgaa gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg
    19141 tcgtctccgg actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac
    19201 gggtaaggct gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga
    19261 aacgcgatta ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc
    19321 tactaccgga atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg
    19381 ctgactactg tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt
    19441 atcccctgag ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata
    19501 ccatattaaa ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat
    19561 ctgggtttct gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag
    19621 ccggtaatac ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga
    19681 tttcgaattc tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac
    19741 accggagtca tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg
    19801 cgcgcgatcg taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt
    19861 aaaggcttcc agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc
    19921 gtctcctgac gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt
    19981 ccgttatcgg ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag
    20041 tgtcaagagg gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa
    20101 tacgctcagt tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg
    20161 attttacgca tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct
    20221 gttaccggag agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga
    20281 tgacttctta tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc
    20341 ctcctcgcca ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat
    20401 cagaacgcct gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg
    20461 tgcggaagca tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac
    20521 ctccccgttt atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg
    20581 ttatgccggt gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc
    20641 ggaggaacat tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg
    20701 catttatcac tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg
    20761 gggcctttcc ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg
    20821 aagcctggct acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt
    20881 cacccgcagc agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc
    20941 gtgaggaata tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc
    21001 taccagaaat ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg
    21061 aatatctact acttccataa tctgacgccc ggttgggggt cattcaatgg tgaaaaaccg
    21121 gagattgcca tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc
    21181 acgccgcccc tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt
    21241 catcatccga tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct
    21301 ctggcaaata aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa
    21361 ctggtctggc tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag
    21421 aacctgaaac ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag
    21481 agcgcagcac tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt
    21541 gacgatagcc gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat
    21601 gatgctcccg gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat
    21661 gacgggcgtc ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac
    21721 ggacagggat gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag
    21781 gtgcggttat tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt
    21841 acgtggcgac agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt
    21901 tccggcatcc gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact
    21961 gttctttcat cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt
    22021 ttaccgggaa ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg
    22081 ttttgttatc agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta
    22141 tcacctgata atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca
    22201 ttgtgctcct tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt
    22261 tctggcgttt ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac
    22321 aaacgaggta ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac
    22381 gtcagtctgc tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg
    22441 taaaaaaagt gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat
    22501 accccctgtc tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc
    22561 acttccatga taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc
    22621 tgcatttcct gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa
    22681 tataccgtta taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa
    22741 gcagtcggag aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa
    22801 caaatgcgta ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg
    22861 tttatgatcc cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca
    22921 tcacactttc agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg
    22981 ccttcctggt ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat
    23041 tttcctggta tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact
    23101 tgcttcaaaa gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt
    23161 cggactcatc gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga
    23221 atttgcggga tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa
    23281 agagtccttt cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt
    23341 atttgaaggg aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata
    23401 tttaccacgc aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg
    23461 acgatataaa aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa
    23521 aaatgcagga agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc
    23581 tcaaagaatt atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg
    23641 ttgaagagta taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga
    23701 gtcccccgcc acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta
    23761 tgaatccgtc tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac
    23821 tttcccgtca catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca
    23881 ttatgggtaa aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg
    23941 tcaccccggt cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg
    24001 gccatggtca cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag
    24061 atgcagagta tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc
    24121 tgattaaagc cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc
    24181 accggggaca tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc
    24241 atgaatggga tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa
    24301 aaacggaggc ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc
    24361 gcaccggctg ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg
    24421 ccgccagtcc cctgatgacc cgcctgcagg acgggctgcg cacggaatct gtccggggtc
    24481 aggcccgtga acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct
    24541 gtgttgcctc tgttgcccgt aaggttctgc gcgctgttcg tgacaccgtg gtctctgttg
    24601 cccgcgcggt atggaactgg attttcttct gaacccggtg gtgatgcacc agactcttgt
    24661 tacagatttt taaccccatg aaaacacaat gcaaacacag ccatacccgg gttgtcccct
    24721 tgcgttgtgt ttctcaagtt aatagaatgc atcgatcgtt actatcatat cgatcgctat
    24781 aaacgatcgt ttgtggtaaa tgacctcctc cgtggttgtc atgaaaatgc gtctcaggaa
    24841 ggttaaataa tgcacgaaaa gaacatcgcc ctgctttgtg atgaagccga ccgacttttg
    24901 caactgaaca ttaatctgct ccggcaaatg gttgatgaac cggatgtgtt atctgacagt
    24961 aagaacgaaa acagactgct ttttgataaa cagaaagcac tgaaaagaat tgaggagctg
    25021 gagggcgaac aaatcaaaac cgcccgcagg gagatggtgc tggctgttgt cggcacgatg
    25081 aaagcaggta aatcaactac catcaacgcc attgtggggc aggaaattct gcctaaccgt
    25141 aaccgcccca tgacctctgt accgacgctc atccgccacg ttcccggaaa aactgagccg
    25201 gtcctccatc tggaacatat tcagcccgtc cgcaatttat taagcacact gcaacaaaaa
    25261 ctcgacaccc cggcaggaca gcaagtcgca cagaccctgc agcaaaccgg ggatacctgc
    25321 gaactgctgg atattctggc aaatgatggc tggctcaaaa atgaatacca cggggaagac
    25381 gaaatcttta ccggactggc atcgttaaac gatctggttc gccttgctgc ggcaatgggg
    25441 agtgagtttc cttttgatga atacgcagaa gtgcagaaac tgccggtgat cgacgtggaa
    25501 ttcagccatc tggtggggat ggatgcatgc cagggaacac tcacactgct ggataccccc
    25561 ggccctaatg aggccggaca accgcagatg gaagtgatga tgcgggatca actgcagaaa
    25621 gcctctgcgg tgctggcggt gatggattac acccagatga actcaaaagc ggatgaagac
    25681 gtccgtaaag agcttaatgc cattgctgac gtatcagccg gccgcctgtt tgtgctggtc
    25741 aataaatttg atgagaaaga ccgcaatggc gatggggcag atgccgtacg ccagaaagtt
    25801 ccggcaatgc tgaacagcga tgtgctgccc gcctcccgcg tttatccagg ctcatcacgt
    25861 caggcgtacc tggctaaccg tgcgcttcat gagttacgga aaaacagaac ccttcctgtt
    25921 gatgaagcct gggtcgatga ttttgtcagg gaagccttcg gtcgcatgaa aaaagaatac
    25981 gtctgtaaag acagtgaaat ggcaaccgaa ggggcgacag acctgtggga aggctcactt
    26041 atcgatcaac tgataacgga agtcatacag agctcccatt ccagagcggc ggcactggcg
    26101 gttgattctg ccgccgcaaa actgatgcag aatgcggaaa atgtcagcga atatctgtca
    26161 ttacgccatc aggggctaca gcaaagcatt cagtcactgc agtcgcatat caccagcctg
    26221 cttgcggata tccgggaaat cgcggactgt caggagcagg tgaccgctga tgtcagaatg
    26281 gccatggagg agatcgatgc cagaacccgg gaattactga cgggggtctg cacctcactg
    26341 gaagaggagc tgaatgacta tttcagaagc ggtaaacgca aagaacagca aatgctggag
    26401 gaagaaaatt cagcgcagcc ccgggagaga aacgcattcg ctttctttca cgatattttc
    26461 ggaaccggaa atcagcatga caggatgcgg gactttgatc cggacagccc cgaaataaaa
    26521 ttcagtgatc gcagggaagc ccttgaactg atgacgcaaa tcgaatcgac cgtgaccagc
    26581 ctgcaccgtg aggctgaagc acagttccgt cctgaactgg aaaaaatcgt caggggtatt
    26641 gaaacaggtt ttcgtggtac ggccctgtac gccacagaaa atattgccgg acgcatcaat
    26701 gcccgtctgg aggatgaagg tttcactgta aaaatcagtt ttccggcagt cagccagtta
    26761 cagacccggc tcgcagtaaa aacaaatctg agcgcgctta tggaggaaag aacggagaca
    26821 gtaacccgtc gctgtcgaca ggatggtgtc tggggtacgc tttgtcgatg ggcaaacacc
    26881 agtgactggg gctggaaaga gtacagtgtg gatgtgagcc gcagcgtgat caatatcaac
    26941 aaggtcagag aggaagtgat gtcactgacc cgggcatatt tcggggagct gcaggcatcc
    27001 attgagcagg atattaacca gcccgtccgc caggaaatcg atgccttttt ctgtacattc
    27061 agggagaaag ttgaacagct gcgtaacacg ctgattcaga gctctgaaga tcataaacgc
    27121 gatcagcagg cgcaggaacg gctgaccggg cgtcttcagg cgttaaatga aagggttcct
    27181 gagctcatta ctgacagtaa ggcgttaagg gaagaactgg agacaatgct gtgacttcac
    27241 catttattca acaaattgct gataaccggg tatgtcaggt actcacctgt cttcctgaaa
    27301 aatttgtggt tgatttcgca aacggtattg atgttgcaca ggagcatatc cgtacggccg
    27361 gagagcgcac gttcttccgg cggttaaaag aggggctgag cggcgaaggt actgcacggc
    27421 agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc tggctgacgg
    27481 agctgacaac gtcacttgcc accacaaact acgcgattac ccgggtaaac gacagggtca
    27541 gttcactggt cagtgatact gccaggctgg cgcattattc cgcagatacg cgggagcagt
    27601 tactcaccct ggccgatcag gttcaccaga aactgaatca tcttgaagaa aaactccacc
    27661 gtgttgacct ggttcagcgg gcgcagttac atcttgagca gatattctcc tggtggagcg
    27721 ccgggcgata tgcgtcattt tcccctgccg gacgttgtta tgtggcactt gaagagcttc
    27781 gctggggcgc gtttggtgat gtgatacgtc agggcgaaac aggccaggtt aaccagctac
    27841 tggatattct cagacataaa gcattaacgc agatggcaca ggagagtggc ggtagtgcaa
    27901 ccgtgcgtct gaacactctg gactggcttg gtggtcaggg ccgggagcag gcagataacg
    27961 agtggcatga cgcgattaac tggctggggg actggtgcag tgaagagcag catccggtga
    28021 tctggtcaac cacgcaggct gcagaacact taccggttcg catgccccgt ctctgctctg
    28081 cagaacgcct ctctgaaagt atggtcgatg aaatatttca gaagggggct gcatgagtat
    28141 agaaataaaa acagggctgg tgctgtccgg cggtggggcg gtgggcgctt atcaggcggg
    28201 agtggttaag gcactggcag agtgtggtac acagatcagc atggtttcag gggccagcat
    28261 tggcgcattc aatggtgcca ttatcgcggc ctctcccgat ctgtcagaag ctgccgtacg
    28321 cctggaggcg ctctgggatc atcttgggaa taatcaggtg ctgtcggtaa accgatcggt
    28381 ttacttttca ttgctgaaaa aattgttcca ggcaatgaac ctctgccaga tccccggacg
    28441 tgcaggagca ctgcttacga cacttcttcg ccatatatcg ataatcaacg gcttcgacaa
    28501 tccgatgact cagccgttgt tgtcagatgg gcccctgaca gcgttgatgg atcattatct
    28561 tgatacagat gctctggcag gcgggctacc gctgtatgtg tcgctgtacc ccacagaagg
    28621 gggcatgcag gatattattg actgcattcg tgctgaactg ggttccggaa ccacgaaaaa
    28681 cgccgttttt cagcatatcc agagtctgcc ccgcggacag cagaaagagg ctctgcttgc
    28741 gtcagccgca ctgcccctgt tgttccgtcc ccgtgaggtt caggggacaa tgttcggtga
    28801 tggtggtatg ggaggatggc gaaatatgca gggaaatacc cctgtaacgc ctctggtcga
    28861 tgccggatgc aatatggtga ttgtgtcgca tctgagtgac ggttctttat gggatcgcca
    28921 ggcttttccg gacaccacaa tccttgagat ccgtccccgg aaaaggctga aatatgcagg
    28981 tgatggtggc aacagtggcg gtctgctcag tttcacgtcg gcacataccg acgcctggcg
    29041 tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac cgctggcagc
    29101 acgtcaggca ctgtcccggt cagaaacggt gttgcagaaa agtctggaga taacggaaga
    29161 ggcagatttg gcactgagaa gcgcgatggc ccggattaaa taaagacgcc ccaaagaaac
    29221 gccaccgcct gtggtggtgt ttttcctgtc tggtgttgtt gaaaatattt tcagatatat
    29281 attacctggg tgagatttaa gattaacgca tttattaatg tcataaggag agttatcatg
    29341 aaattcatcg taaaagcact taggtgatgc tgccaactta ctgatttagt gtatgatggt
    29401 gtttttga
//
LOCUS       DAJTNW010000050        29079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNW010000050 DAJTNW010000000
VERSION     DAJTNW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(172..1068)
                     /gene="yhaJ"
                     /locus_tag="OO179_002265"
     CDS             complement(172..1068)
                     /gene="yhaJ"
                     /locus_tag="OO179_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5532304.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1173..1874
                     /locus_tag="OO179_002266"
     CDS             1173..1874
                     /locus_tag="OO179_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5532305.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1897..2061
                     /locus_tag="OO179_002267"
     CDS             1897..2061
                     /locus_tag="OO179_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532306.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2273..3583)
                     /locus_tag="OO179_002268"
     CDS             complement(2273..3583)
                     /locus_tag="OO179_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5532307.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3611..4942)
                     /locus_tag="OO179_002269"
     CDS             complement(3611..4942)
                     /locus_tag="OO179_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5532308.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5217..6581)
                     /gene="tdcG"
                     /locus_tag="OO179_002270"
     CDS             complement(5217..6581)
                     /gene="tdcG"
                     /locus_tag="OO179_002270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5532309.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6653..7042)
                     /locus_tag="OO179_002271"
     CDS             complement(6653..7042)
                     /locus_tag="OO179_002271"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5532310.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7056..9350)
                     /gene="tdcE"
                     /locus_tag="OO179_002272"
     CDS             complement(7056..9350)
                     /gene="tdcE"
                     /locus_tag="OO179_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5532311.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9384..10592)
                     /gene="tdcD"
                     /locus_tag="OO179_002273"
     CDS             complement(9384..10592)
                     /gene="tdcD"
                     /locus_tag="OO179_002273"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5532312.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10618..11949)
                     /gene="tdcC"
                     /locus_tag="OO179_002274"
     CDS             complement(10618..11949)
                     /gene="tdcC"
                     /locus_tag="OO179_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5532313.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11971..12960)
                     /gene="tdcB"
                     /locus_tag="OO179_002275"
     CDS             complement(11971..12960)
                     /gene="tdcB"
                     /locus_tag="OO179_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5532314.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13059..13997)
                     /gene="tdcA"
                     /locus_tag="OO179_002276"
     CDS             complement(13059..13997)
                     /gene="tdcA"
                     /locus_tag="OO179_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5532315.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14187..14531
                     /gene="tdcR"
                     /locus_tag="OO179_002277"
     CDS             14187..14531
                     /gene="tdcR"
                     /locus_tag="OO179_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5532316.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14787..15326
                     /locus_tag="OO179_002278"
     CDS             14787..15326
                     /locus_tag="OO179_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532317.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15348..15695
                     /locus_tag="OO179_002279"
     CDS             15348..15695
                     /locus_tag="OO179_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532318.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15864..16514
                     /locus_tag="OO179_002280"
     CDS             15864..16514
                     /locus_tag="OO179_002280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532319.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17022..17398)
                     /gene="rnpB"
                     /locus_tag="OO179_002281"
     ncRNA           complement(17022..17398)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO179_002281"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17431..18576)
                     /gene="garK"
                     /locus_tag="OO179_002282"
                     /pseudo
     CDS             complement(17431..18576)
                     /gene="garK"
                     /locus_tag="OO179_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18673..19563)
                     /gene="garR"
                     /locus_tag="OO179_002283"
     CDS             complement(18673..19563)
                     /gene="garR"
                     /locus_tag="OO179_002283"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5532320.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19593..20363)
                     /gene="garL"
                     /locus_tag="OO179_002284"
                     /pseudo
     CDS             complement(19593..20363)
                     /gene="garL"
                     /locus_tag="OO179_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20379..21713)
                     /gene="garP"
                     /locus_tag="OO179_002285"
     CDS             complement(20379..21713)
                     /gene="garP"
                     /locus_tag="OO179_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5532321.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22088..23659
                     /gene="garD"
                     /locus_tag="OO179_002286"
     CDS             22088..23659
                     /gene="garD"
                     /locus_tag="OO179_002286"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5532322.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23711..24535)
                     /gene="agaR"
                     /locus_tag="OO179_002287"
     CDS             complement(23711..24535)
                     /gene="agaR"
                     /locus_tag="OO179_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5532323.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24784..26064
                     /gene="kbaZ"
                     /locus_tag="OO179_002288"
     CDS             24784..26064
                     /gene="kbaZ"
                     /locus_tag="OO179_002288"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5532324.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26087..26560
                     /gene="agaV"
                     /locus_tag="OO179_002289"
     CDS             26087..26560
                     /gene="agaV"
                     /locus_tag="OO179_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5532325.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26571..27350
                     /locus_tag="OO179_002290"
     CDS             26571..27350
                     /locus_tag="OO179_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5532326.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27340..28149
                     /locus_tag="OO179_002291"
     CDS             27340..28149
                     /locus_tag="OO179_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5532327.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28236..28670
                     /locus_tag="OO179_002292"
     CDS             28236..28670
                     /locus_tag="OO179_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5532328.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28667..>29079
                     /locus_tag="OO179_002293"
     CDS             28667..>29079
                     /locus_tag="OO179_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5532329.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7565 a         7591 c         6865 g         7058 t
ORIGIN      
        1 tgacaaagtg cgctttgttt atgccggatg cggcgtaaac gccttatcca gcctacaaat
       61 tgccaaaatt caatatattg cagtgactgc gtaggcctga taagcgtagc gcatcaggca
      121 gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg tgcttaatag attattttcc
      181 agcgaacaac tttggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
      241 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
      301 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
      361 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
      421 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
      481 tttcacacgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
      541 ggcaacgtag acgttcatta acgtatagag cttgcgcgag ttgatctccg acgaggaacg
      601 aaaatgcata tccggcgcga taacgatatc cgcccgcccc tgctccagcc gttcccacgc
      661 ccccgccagc acttctgtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
      721 gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt
      781 ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt ttatctgcgg cttccagcaa
      841 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga
      901 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
      961 ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa ctgccccggc gatcgatcgc
     1021 atccataacc cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt
     1081 caggaaattt gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta
     1141 ccataaaagg aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt
     1201 gtggacaagc agactacgga tggttgcagg cccggtatac tttttccttt ggacactact
     1261 tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct taaccaggaa gtgctggccc
     1321 caggtgccgc ctttcagccg cgaacctatc ccaaagtcga tattttaaat gtgattctgg
     1381 atggagaagc agagtatcgc gacagcgaag gcaatcatgt tcaggccagc gccggagagg
     1441 cgttgctgct ctctacccag ccgggtgtca gctatagcga acacaatctc agcaaagaca
     1501 aaccgttaac gcgaatgcag ctttggctgg atacctgccc gcagcgagag aatccgctga
     1561 ttcaaaagct ggcgcttaat atggacaagc agcaattaat cgcctcgcca gagggtgcga
     1621 tgggaagcct gcaattacgc caacaagtgt ggctgcacca tatcgtgctc gacaaaggcg
     1681 aaagtgcgaa tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat
     1741 ttcatgcgct tacgcatcat gaagagaaag cggcgctaac ctgcggtgat ggggcgttta
     1801 ttcgtgacga ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag
     1861 atttgcctgt ctagttgttt ttacagggag atgatgatga gtaaaaaatc gaccaaaaag
     1921 cgccagccag cgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa aaattttggc
     1981 tatgaagaga tgttgagcga gctggaagct atcgtcgcgg atgctgaaac gcgtttagcc
     2041 gaggatgaag ctaccgcgta aagacaggtt gccggatgcg gcgtaaacgc cttatccggc
     2101 ctacaaaaca caacaacatc aacaaattaa acaccccgta ggccggataa gatgcgccag
     2161 catcgtatcc ggcatcaggt acaaaacaca acaacatcaa caagttgagc accccgtagg
     2221 ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc gtttatctgg
     2281 ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta
     2341 acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg
     2401 tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg
     2461 aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga
     2521 tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc catgccatcc
     2581 cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa cgcggtaact
     2641 ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agtcgttcat
     2701 catccgctcc gaagtgttct gctacaacca ccacaggcat tattgcagtg atcccctggt
     2761 tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg
     2821 ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc
     2881 actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg
     2941 ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca aatgggactt
     3001 cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact
     3061 cttgctcgcc ctctgccaca cacgcctggt gggtaaacac cacaccattg tgcgtctcaa
     3121 tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc
     3181 agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta acagagactt
     3241 tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca
     3301 cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc
     3361 ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa
     3421 cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca
     3481 gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga
     3541 ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat
     3601 ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca aagcaacaaa ccagtgacga
     3661 taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc
     3721 acgccgggat caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca
     3781 acaccggtgc gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc
     3841 cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca
     3901 ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga
     3961 cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa
     4021 taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt accgcttcgt
     4081 catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc
     4141 caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc
     4201 gcgagcgata agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg
     4261 taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga
     4321 cgttatacag atgccacatt cccaccatcg acacgcccag cgcagcgact accagcagct
     4381 tggtcagcac catgtcggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg
     4441 ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg
     4501 tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga
     4561 acatccaaat caccagcatt acgaaataga gcgcacctaa caggatgccc cagtttttac
     4621 ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat
     4681 taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa
     4741 atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg
     4801 ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc
     4861 gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc
     4921 ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt
     4981 ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa
     5041 agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttaa aataacggag
     5101 atggataaga attttctact aaattaatcg caagagaata ttcattctct atttgcggca
     5161 gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag
     5221 ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata
     5281 tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag
     5341 gtgcggcgca tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt
     5401 tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc
     5461 tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt
     5521 aaccctgccg ctgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg
     5581 ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag
     5641 cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc
     5701 ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt
     5761 tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta
     5821 gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgaca cggcacattg
     5881 agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact
     5941 tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg
     6001 tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct
     6061 gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct
     6121 tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt
     6181 cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag
     6241 ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc
     6301 ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga
     6361 ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt
     6421 gacaacgacc cgtacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt
     6481 tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag
     6541 ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc
     6601 cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac
     6661 tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca
     6721 cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat
     6781 tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca
     6841 cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgtgcctgat
     6901 cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga
     6961 agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt
     7021 gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt
     7081 gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag
     7141 acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc
     7201 cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa
     7261 tacccatcca gcaggccaac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc
     7321 gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc
     7381 gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg
     7441 cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg
     7501 tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt gcgataggtt
     7561 ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg
     7621 cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg
     7681 ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc
     7741 gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc
     7801 gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg
     7861 ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg
     7921 taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc
     7981 tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca
     8041 aagaactgca tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg
     8101 tcgctgttga agtcagtacg catcagatcg tcattttcat actgcaagga agaggtgacg
     8161 atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg
     8221 gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag
     8281 tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag
     8341 attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg
     8401 atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag gacgccagct
     8461 ttaaagtcgc gctcaatgta gatatccagg aacgatgccg tgcgccccag cgacatcgcg
     8521 ccgccatttt gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc
     8581 tgtgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc
     8641 tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga
     8701 tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgcacc
     8761 agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacgacca
     8821 tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag
     8881 taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat
     8941 tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg
     9001 aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc
     9061 ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa
     9121 tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacctt ttcccacaat
     9181 tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat cgccttcata cggtgtatag
     9241 ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc agtccgtacc tttaaagcca
     9301 agccatgcgt cagcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat
     9361 ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac tttacctaaa
     9421 tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacgac gcaaatgaca
     9481 tgcgcatttt cactggaaac aattcgctcg ccaaaagagt tagatcgatt attcatttct
     9541 gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaaactt
     9601 gaattctctc ctattccgcc ggtgaatata attccatcca gccgacgtaa tgaagctgca
     9661 tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt
     9721 tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata
     9781 cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt
     9841 tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc
     9901 aagccttcca gcggcgtcat tcccattgag gtgtcaacac tctgaccgtt gcgaactgcg
     9961 cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga
    10021 ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa
    10081 cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga
    10141 gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat
    10201 tgctgcgccg attcaatacc acttaaattt gcgtaattat gcaggggtgc cagtggagaa
    10261 acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg
    10321 ccgccgtgag cgatgcggtg gccaattaag gccacgctgt catttaaatt ccgtttttcc
    10381 agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc
    10441 tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac
    10501 attaatactt cacaatcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag
    10561 ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta
    10621 aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt
    10681 atccagacga ccacggtatt tcgccagaga cggcgcttta cggatagcat acatcggcaa
    10741 caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt
    10801 cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgagagtatt
    10861 cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc
    10921 attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag
    10981 tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttcg taccggtcat
    11041 ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc
    11101 catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca ccactgcaac
    11161 catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc
    11221 gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa
    11281 gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg
    11341 accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
    11401 gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat
    11461 cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa
    11521 cagcgccaca aagccgcgat tcagcggtgc aaatccgagc tggttttccc agaacgtcat
    11581 aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta
    11641 caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat
    11701 gttgccggaa gggttagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa
    11761 tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc
    11821 gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca
    11881 tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga
    11941 agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga
    12001 gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat
    12061 atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt
    12121 gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac
    12181 cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga
    12241 gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg
    12301 gaaagaagcc gccatgccgt gaacgttttc tgactgtacg ccaataacac gaatggtcgg
    12361 gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccaccaccac caattggcac
    12421 aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc
    12481 agcaatcact ttcggatcat cgtaaggtgg gataaaaata cgaccttcca tttcgacaat
    12541 ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga cttctgcgga
    12601 gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc
    12661 gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca
    12721 ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat taaatgcgcc
    12781 acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt
    12841 gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc gcccagccag
    12901 tcgttgtttc gcttcaataa tatcatcaat agcaaccggc agatcgtatg taatatgcat
    12961 tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca
    13021 gacaggtgga ttatttatgt ttaaataaaa acaaacaact aaccaacttc tattaattgc
    13081 cttcgtctac acccattata agatgaatac tctttggcta attccaccaa aaccgatgct
    13141 gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac aggtaatgtt
    13201 tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat atcacaagga
    13261 attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt
    13321 ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag ttcgctgtag
    13381 taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg
    13441 gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc gaacagcggc
    13501 tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt
    13561 cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc
    13621 gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt aaaaccaatc
    13681 aatgaaggaa aaccaaatga gacttccacc accgcttcag aagacatacc gcttatctca
    13741 ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg
    13801 gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct
    13861 tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc ttttgcagcc
    13921 gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga
    13981 agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc
    14041 aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt aactttcatt actttccaag
    14101 gtatcgttat tacaattaaa tatctttaaa tatcaacaag ttaaagcata aaaatcagca
    14161 taaaccctga tttttatcaa aaaaaaatga cggggataac catattttat ggcgataaca
    14221 tcattcgtta tgtggttaat acaaaaaagg ggctgagacc atatttcaag caattaccgg
    14281 ataattacca ggcaaaattt gagttaaatt taatgagcaa gttctcaaat tttattataa
    14341 ataaaccatt ttcagcgata aatactgcgg cacgtcacat tttttcacgc tatttgttgg
    14401 agaacaaaca tttattttat caatatttta aaatttcgaa tacaggtatt gatcatctcg
    14461 aacaattgat taacgtcaac tttttctctt ctgacaggac gtcattttgt gaatgcaatc
    14521 gttttccata aattcttctc ccctcatagg cgacgaatag cattttgtgt tgaggatcac
    14581 aaaacgaata attgctggtc gccgcgataa ggtcagacga agtcaacaag ggggattttc
    14641 actgtgcttt acatcagcgt aggacacaga atgttgcttc gtttactgta acgccgggta
    14701 aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga aaataagcat ctattattgt
    14761 ttgtcatgac gacaaggcag gcatcaatga aaggcttccc aattgcacat atttttcacc
    14821 cttcaatccc gccaatgcac gcagtggtta acaatcacaa tagaaatatt gattattgga
    14881 cggtaaaaag aaagtttgca gaaattgtct ccaccaatga cgttaataaa atttacagta
    14941 taagtaatga actccggaga gtattatctg caattactgc attgaatttc tatcaaggcg
    15001 atgttccttc tgtcatgatc cgaatccaac cggaaaatat gagtccattc atcatagata
    15061 tttctacagg agaacatgat gattatatca tacaaacatt agatgtaggc acttttgcac
    15121 cttttggtga acaatgtact tgctcagccg tcaataaaaa agagctggaa tgtattaagg
    15181 agacgatttc taagtattgc gcaaagttca cccaaaaaga agccatctta acacccccta
    15241 ggcactttaa taaaacctgc attacttcag attgttggca aattctcttt ttttcacccg
    15301 atcattttaa taatgatttt tattgattat ttataaagga taaaataatg ttccctgttt
    15361 catcaattgg taatgatatt agtagtgatt tagtccgtcg aaaaatgaat gaccttcctg
    15421 aaagccccat aggaaataac ctcgaagcac tagcaccagg tatagaaaaa ctaaaacaga
    15481 cctctattca aatggtcact ttacttaacg cgttacaacc aagtggaaaa tgcattatca
    15541 ctggtgattt tcaaaaagaa ttagcgtact tacaaaatgt aattctttat aatgactcgt
    15601 ctcttcgtct ggatttttta ggttataaca cccaaattat tcaacgatcg gacaatacct
    15661 gcgaacttac cattaatgaa ccgttaaaaa actaggaaat atctacaggc aatatcaatg
    15721 ttaattgccc attaaaagat atttacaatg aaatcaggag gttgaacgta atctttagtt
    15781 gtgggactgg agatatagtt gatctatcct ctctggactt acgtaatgtc gatttagatt
    15841 attatgattt cacagataaa catatggcta atactattct aaatcatttt aaattggatt
    15901 caacaaattt tactaatgcc aacatgtttc aggttaattt tgttagttca acacaaaacg
    15961 ccacaatctc ctgggattat ttactaaaaa taacgcctgt tttaataagc attagcgata
    16021 tgtattctga agaaaaaatc aagtttgtcg aaagttgttt aaatgagctt ggagacatta
    16081 ccgaagaaca attaaaaatt atgagatttg caattataaa atctatacca agggcaactc
    16141 ttacagataa attagaaaat gaattaacaa aagaaatata taaaagctca tcgaaaatca
    16201 acaattgctt gaacagaatt aaattaccag agatgaaaga attctcatca aaaaaaatat
    16261 atgattacat cgatacaatc attgaagatt atgaaaatat caaagaaaat gcttatctgg
    16321 tcgtccccca aattaattat actatggatt taaacataga agactctagc tcagaagagt
    16381 taataccctc gagaaagacg aaaattctcc ggacaatggc tttgaggtcg gggaatataa
    16441 cacatatgaa gcatataact cagagaatca atattttacc agagaggact atacgtataa
    16501 ttacgacctt ttaaatgcaa tatagactta ataacttctt acaaagtcta ttattaatac
    16561 cgtagatatt catcttaccc tgtgcaccaa aagcaaaaac ttatcgacgc gggtttgaaa
    16621 ctgatggcaa ccgcaaaaat gcctgatgcg ctacgcttat caggcctacg ccatctctgc
    16681 aatatattga atttgcgtgc ttttgtaggc aggataaggc gttcacgcag catccggcat
    16741 gaataaagcg cactcaacaa tctgaaaccc gccggaacgg tttattacgt acatcaggtg
    16801 aaactgaccg ataagccgct ttcttttggg tatagtgtcg tggacagtca ttcatctttc
    16861 tgcccctcca aaagcaaaaa cccgccgaag cgggttttta cgtatatcag gtgaaactga
    16921 ccgataagcc gctttctttt gggtatagcg tcgtggacag tcattcatct ttctgcccct
    16981 ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaaa caggtgaaac tgaccgataa
    17041 gccgggttct gtcgtggaca gtcattcatc taggccagca atcgctcact ggctcaagca
    17101 gcctacccgg gttcagtacg ggccgtacct tatgaacccc tatttggcct tgctccgggt
    17161 ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc gcaccctttc
    17221 acccttacct gatcccgctt gcacgggcca tcggcggttt gctctctgtt gcactggtcg
    17281 tgggtttccc ccccaggcgt tacctggcac cctgccctat ggagcccgga ctttcctccc
    17341 ctccgcccgt ctcccccgaa gaggacgacg acgaagcggc gactgtctgg tcagcttcgg
    17401 cgcgcagtat agagggtttg cgcgcccttg tcaccccgtg ttgcgcattc caatcgccag
    17461 tgtcgcggca atattacgtg aagcacggca gatattgtca taagccccgc ggaatgcttc
    17521 gtccaacgta cctatgctgg tcaatacgct gaagaccgca tcaatgccat gctgatgtac
    17581 aacgccaaca tcatcggtca ggctacccgc aatgccaatc accggtttat ggtacttctt
    17641 cgccacgttt gcgacgccaa tcggtacctt gccgtgaata ctctggctgt caatacgccc
    17701 ttcaccggtg atcaccagcg tacaatcgtg aatatgttcc tccagattca gcgccgtagt
    17761 gacgatttca ataccacttt tcagttccgc accaagaaac gccatcagca ccgcacccat
    17821 accgcccgca gctcctgcgc cggggacatc ttttacatca acatgcagcg cttttttaat
    17881 gacatcggca tagtgagaga ggttattgtc cagctcaaca atcatcgctt cactggctcc
    17941 cttttgtggg ccaaagatgc gcgatgcgcc gttatcgccc accagcggat tggtgacatc
    18001 acaagcgacg cgaatgacgc aatcttttaa gcgcggatcg aggccggaaa tatcaatatc
    18061 attcagagta ttaagactac cgccgccaaa accaatttca ttgccgttgg cgtcgcataa
    18121 tttcgccccc agcgcctgta ccatgcctgc gccgccatca tttgtagcgc taccgccaat
    18181 gccgataata atgtttgtcg cgccgctctc cagcgcctac aggattaact cgcctgtgcc
    18241 gcgtgaagtg gtcacgagtg gatcgcgttt ttccgcaggt accagctcca gcccactggc
    18301 cgccgccatt tcaataaacg cggttttgcc atcgccggag atcccccaac tggcattcac
    18361 tttctcgccc agcggccctg taacccaggc gtgacgttca gaaccctggg tggctgcaat
    18421 catcgcttcc accgttcctt cgccaccgtc ggcaaccgga atagaaacgt actgtgcatc
    18481 aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca acctcgctgg cagataaact
    18541 ttctttataa gagtctgggg cgattacgat tttcatacct atgcctgtta ccacatgacg
    18601 ccggagggcg tttctcttat tcggcctgga ttccaggccc ggattgcaat acgccatccg
    18661 ggcacgacgt cattaacgag taacttcgac tttcgccagt ttttcgtagt agcacgccag
    18721 ggcgctatga tccgccgttc ctaaaccatc tgctcgcagt gcctgcatca tctccataac
    18781 cgcagctgtg agcggcagtt gcgcgccgac gccgtgggaa gtatccagcg catttgccag
    18841 atccttaata tgcagatcaa tacggaagcc cggcttgaag ttgcggtcca tcaccatcgg
    18901 cgctttggca tccagcacgg tactgcccgc cagtccaccg cgaattgcct gatacaccag
    18961 gtccgggtta acgcccgctt tagttgccag cgttaacgct tctgacatcg cggcaatatt
    19021 cagcgccaca atgacctgat tcgccagttt ggtgacgtta cctgcaccaa ttgccccggt
    19081 atgcaccacg gaacccgcca ttgctttcat caaatcatag tatttgtcga aaatagcctt
    19141 gtcgccgccc accatcactg acagcgtacc gtcgatggct ttcggttcac cgccgctcac
    19201 cggagcatcc agcatatcaa tgcctttcgc tttcagcgct tcgctgattt cacggcttgc
    19261 cagcggtgcg atagaactca tatcgatcaa taccgtacct ggcttcgcgc cttcaataat
    19321 gccattctca cccagcgcca cctctttcac atgaggggag tttggcagca tggttatgat
    19381 gacgtcgcac tgttcagcga tcgctttagc cgtagaggct gtttctgcac ctgcagcaat
    19441 cacgtcagca atagcttctg ggttacggtc agcaaccacc agcgagtaac ctgctttcag
    19501 aaggttttta ctcattggtt tacccataat ccccaggcca ataaaaccaa ctttcatagt
    19561 catatcaatc atctctcttg ttgcggtggt ggttattttt taaaggtatc agccagttta
    19621 tgagtggcag agcggaagac gccgagatcg ctgccgacag ccacaaacgt cgcgccccat
    19681 tccagataac gacgcgcatc ggcttcgacc ggcgcgagga taccgctggg tttgccgtgc
    19741 gcgctggcac ggttaaaaat gtgctgaatt gctttttgta catccgggtg tgatgcattg
    19801 ccgagatggc ctaatgccgc ggccagatcg ctggggccga cgaagatgcc gtctacgcct
    19861 tcggtagcgg caatggcatc gacgttatct acgccctgct gactttctat ctggaccaga
    19921 atagtgatgt tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa catattggcg
    19981 cggtgagaaa cggagacgcc gcgaatgcct tccggtgggt aacgggttga tgccaccgcc
    20041 tgctctgctt cctcttttgt ttctacaaaa ggaatcagga agttatagaa accgatatcc
    20101 agaagacgct taataatttc cggctcgttg gtcggcactc acactactgg cgcgctggcg
    20161 ctgcctttca aggccattaa ctgcggaata aacgtggaga tatcgtttgg cgcatgttcg
    20221 ccatccagca ccagccagtc aaacccagcc aaaccaagaa cttcagtgct aatcgggtta
    20281 gagagtgctg accagcaacc aatttgtacc tgtttcgcag ccagtgcggc tttgaattta
    20341 ttcgggaaaa catcgttatt catcgcttat acctttgctt atttctgcaa ttccatacgt
    20401 ttaatgtcgc caactacgaa gaggtagcag accatcgcca tcagcgctga acatcccacg
    20461 aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca ggtagccaat caccagtgga
    20521 gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc cgccacagag gccaacaatc
    20581 tctttcggcg cggtgtcaga aatcaccggc cagcccagag caccaaatcc tttgccaaaa
    20641 aaagccagcg ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt acataagatg
    20701 atggtggaag ccagcaacat tcccagcaca atcggtagct tacgtgccag ggtcagggat
    20761 aaaccgcgtt tgatcagata atccgagaag acacctccca gcacgccgcc cgcaaaacca
    20821 cacagtgccg gaatcgaggc gaccagaccc actttcagaa tcgacatgcc tttttcctgc
    20881 accagataaa tcgggaacca ggtgaggaag aaccaggtga tggtgttgat aaaatattgt
    20941 ccgaaaaata cgcccaacat catgcggtta gagagcaatt gcttgatgta atgcagtttg
    21001 agaccgcttg ctgccgcgct gcccggcttt ttgtggtcca tatcgaccac cgcgccattt
    21061 tcagagataa acttcagctc ttcctcagac atacgtgggt gatctgtcgg gttatgaatc
    21121 aacttgatcc acagcgccgt cagcacaaaa caaatcaccc ccataacggt aaagacgtgc
    21181 tcccatcccc aggcgaaagt cagccagcca agcagaggcg aaaagagcgc cagcgagaaa
    21241 tattgtgccg agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg gaaccaggcg
    21301 gcgacaatac gggcgttcgc cgggaatgat ggagcttccg agaatcccag cataaagcgc
    21361 ataaagaaca tggagatccc tgcccaggcc agcgggaaca tatcaacaaa gccttgcagg
    21421 aaggtgaaca gcgaccagaa aaagaggctg taggtgtaaa cttttttcga gccaaactta
    21481 tcaagcaacc agccgccggg gatttgcatc agcaagtagg cccagccaaa agcggagaag
    21541 atgtaaccca tcgaaaccgc acttaactgc aactcttttg ccacttcggt accagcaata
    21601 gacagcgttg cacgatcggc gtagttaacg gcggtaacaa taaaaataat cagtaatatt
    21661 aaatagcggg tatgcacacc tttccttttt tcgtcaacgg tgtccagaat cattttattt
    21721 acctcgggta cttatgctga tttttattat tatggggaag gtgttattta taagtttcat
    21781 ttatgccgta acgacaatga actcgggaat tagtataagc agcgcgagaa taataatcat
    21841 tgtgcaaatg ctaatttaat taatactttt taaatattat tttgagcata tgcacataag
    21901 gttgcgcgct aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctgaa
    21961 atgattgaca ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg
    22021 aaatcattta tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata
    22081 ttgcacaatg gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt
    22141 tcacgacaca gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg
    22201 ttttccggat gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct
    22261 ggacattccg gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg
    22321 tgcaatccca cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc
    22381 gttacacacg ctgccactgg caaccaaagt cccggaaccc ttaccgccgc tggaaggata
    22441 cacctttgag ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat
    22501 caccaccagc gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg
    22561 cgatctgcta ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg
    22621 ttgtggcgtg gcgattaacg caccggcagc agttgtacct attcgtacca ttcacaatat
    22681 ttcgctgaac cctaactttg gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt
    22741 gcagcctgag cgcctgctga ctggaacgga tgatgtgcaa gctattccag tagaaatcgc
    22801 cagcattgtc agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat
    22861 tttgcaggtc gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc
    22921 ggcttcagaa ctggttgtcg gcatgcattg cggtggcagc gatgcgtttt ctggcgtaac
    22981 ggcaaacccg gcagttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat
    23041 gttttcagaa gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa
    23101 cgaagaggtc ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat
    23161 gggaaaaacc gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa
    23221 cgtggtagag aaagcgctcg gctccattgc taaatcgggc aaaagtgcaa ttgttgaagt
    23281 gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag
    23341 cgattttgtc tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac
    23401 cggtcgtggt acgccgtacg gcctgatggc ggtacccgtc attaaaatgg caacccgcac
    23461 cgagctggcg aaccgctggt ttgatttaat ggatattaat gcgggcacca tcgctaccgg
    23521 cgaagaaact attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag
    23581 cgggaagaag aaaaccttct cggatcaatg gggactgcat aaccagctgg cggtgtttaa
    23641 cccagcaccg gtgacctgat aattcaattc gtattatgcc cgatagcaca cgtttatcgg
    23701 gcatattgag tcacagcgaa gacgcgtgat ccccgaccag aatcacttca accccagcct
    23761 ttcgcagtcc ttccagacta tccgcaggaa tgccttcatc aacaatgatc atgtcgatac
    23821 gttgagtatc aatgatctta tgtaaactgg aacgattgaa tttactggaa tcggtgacca
    23881 cgatgatccg ttccgcaact tcgcacatcc gacggtttaa acgggcttca tcttcattat
    23941 gtgtgctgac gccgcgctcc agatcgatcg catctacacc aagaaacagc atatcgaagt
    24001 ggtaattttg cagcgattgc tcagcctgat cgccgtaaaa agattgcgac tgacggcgca
    24061 aatgcccgcc ggtcatcagc agctcaacgc cttccgcttc cagcaacgca ttagccacgt
    24121 tcataccgtt ggtcatcgca attacgtcag tgtacttgcg catcagacga gcaatctcaa
    24181 aagtggtggt cccggaatcg aggatcaccc gatgacctgg ctgaatcaac tcaacggcag
    24241 ctttcgcaac gctgcgtttc atcgcggtgt tcagtgcgct tttatcttcc actgatggct
    24301 cgactgacgg cgtcgtgcta tcgcagatca acgcgccacc ataggcacgc acagcgatcc
    24361 cctgcttttc cagaaacgcc agatcattgc ggatcgtcac agtagatacg ccatacaatg
    24421 ccgacagatc gttaacctgc acactccctt gctgtcgcag acgctgaatg atctgttctc
    24481 gtcgctcgct ggtgcctgtc actcgcttct cacctgaagc gtcggtatta ctcatagtaa
    24541 gtcctttcgt aaaactttcg tttcatttcg ttttgcctat taacgccttt ctattaagca
    24601 aatgcaagcc caccttgccc attggcgcaa gctactctcg tttcagtgac tttcattacg
    24661 tttcttttgt gaatcagatc agaaaactat tatctttcgt tttattttta tctcaccatg
    24721 acgcagtatc aattgaaaca aaacgaaaga tcaatatcgc agtaatctga actggagagg
    24781 aaagtgaaac atctgacaga aatggtgaga cagcacaaag cgggcaaaac aaatgcaatt
    24841 tatgccgttt gttccgcaca tccgctggtg ctggaggctg caatccgcta cgccagtgca
    24901 aaccaaacgc cgttactgat tgaagcaacc tccaatcagg tagaccagtt cggcggttat
    24961 accggaatga cgcccgccga ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat
    25021 ttcccgcagg atgcgctgat tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac
    25081 ctgccagccg ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta cgttgcggca
    25141 ggattcaaaa aaatccacct tgattgcagc atgtcctgtc aggacgatcc aattccctta
    25201 actgatgaca tcgtggctga acgcgccgcc cgtctggcga aagtggcgga agaaacctgt
    25261 cgcgaacact ttggcgaagc cgatctggag tatgtcattg gtaccgaagt gccggtacct
    25321 ggcggcgcgc atgaaacctt aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc
    25381 acgctggaag cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc
    25441 attattgccc tggtggttca acccggcgtc gaattcgatc acaccaacgt tattgattat
    25501 cagcccgcca aagcgagcgc cttaagccag atggtcgaaa actacgaaac gctgattttc
    25561 gaagcgcact ctaccgatta tcaaacgccg caatctctgc gccagctggt gattgaccac
    25621 tttgccattc tgaaagttgg cccggcgctg accttcgccc tgcgtgaagc ccttttttct
    25681 ctggcggcga ttgaagaaga actggtacca gcgaaagcct gttccggtct gcgtcaggtg
    25741 ctggaaaacg tgatgctcga ccgcccggaa tactggcaaa gccactacca cggtgacggc
    25801 aacgcgcgtc gtctggcgcg tggttacagc tactcagatc gcgtgcgcta ttactggccg
    25861 gacagccaga ttgatgacgc tttcgctcat ctggttcgta atctggcgga ttcaccaatt
    25921 ccgctaccgc tcatcagcca gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag
    25981 ctgcaaccga cgccacggga actcattata aaccatattc aggacatcct ggcgcagtac
    26041 cacacagcct gtgaaggcca ataagcaaaa caaagaggaa catgctatgc caaatattgt
    26101 tttaagccgg attgatgaac gcttgattca cggtcaggtc ggcgttcagt gggtcggatt
    26161 tgcgggggca aatctggtgc tggtagccaa cgatgaggtt gccgaagatc cggtacaaca
    26221 aaacctgatg gaaatggtac tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa
    26281 agttatcgac aacattcatc gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac
    26341 acccgccgat ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg
    26401 caatatgcac tacgccaatg gcaaacaaca aatcgccaaa acggtttctg tggatgcggg
    26461 cgatatcgca gcatttaacg acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt
    26521 cccgacagag cctgctgtgg acctctttaa attactttga gggattcatc atggaaatca
    26581 gcctgttgca ggcatttgcg ttgggcatta tcgcctttat cgctggccta gatatgttta
    26641 acggcttaac ccatatgcac cgcccggtgg tcctcggccc gttggtcggg ctggtacttg
    26701 gcgatctgca taccggaatt ttaaccggcg atacgctgga actagtgtgg atggggctgg
    26761 ccccgctggc gggcgcacag ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt
    26821 ttgccattac tactggcgtg aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg
    26881 tcgcagtaca gatggggatt accttcctgt tctcggtgat gtctggcgtg atgtctcgct
    26941 gcgaccggat ggcggagaac gccgatactc gcggcattga acgcgtgaac tatctggcac
    27001 tgctggcact cggcactttc tattttctct gcgctttcct gcctatctac ttcggcgcgg
    27061 aacatgcaaa aaccatcatt gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg
    27121 cgggcggcat catgccagca atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg
    27181 tctacatacc ctacttcatc ctgggctttg ttgccgcaac ctggctcaag ttaccggtgc
    27241 tggctattgc tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg
    27301 aaccgactca acctgcggca cagaaagagg aatttgaaga tggcatctaa tcaaaccacc
    27361 ctgccgaacg tcagcgaaaa tgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat
    27421 gaagatcgga gcattgccgc ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt
    27481 tccatgctgt tacaagcgtc ttttaactac gaacgtatgc aggcttctgg ctggttgtac
    27541 ggtctgctgc ctgctctgaa aaagatccac actaataaac gcgacctggc gcgcgccatg
    27601 aaggggcata tgggtttctt caatacccat ccgtttctgg tgacgtttgt tatcggcatt
    27661 atccttgcga tggagcgttc taagcaggac gttaacagta ttcagagcac caaaattgcc
    27721 gtcggtgcgc cgcttggcgg gattggcgat gcgatgttct ggctaacgct actgccgatt
    27781 tgtggcggga taggcgcaag cctcgcgcta caaggctcta ttcttggcgc agtcgtcttt
    27841 attgtgctgt tcaacgtggt acacttgggg ctgcgttttg gtctggcgca ttatgcttac
    27901 cgcatgggcg tggcggcgat tccccttatt aaagcgaata ccaaaaaagt cggccatgct
    27961 gcgtcaatcg ttggaatgac ggtaatcggc gcactggtgg caacctatgt tcgtttaagc
    28021 accacgctgg aaatcacggc gggcgacgca gtagttaagt tacaggctga tgttatcgac
    28081 aaactgatgc ctgccttctt accgctggtc tacaccctga ccatgttctg gctggtacgc
    28141 cgcggctaga gtccgctgcg cctgattgcg gttaccgtgg ttctagggat cgtcggtaaa
    28201 ttctgccatt tcctttaaat acaatgaggt attagatgtt aagtattatt ttgacagggc
    28261 atggcgggtt tgccagtggc atggaaaaag caatgaagca aatcctcggc gatcaatcgc
    28321 agtttattgc catcgatttt cccgaaacct caagcgccgc gctgcttaca tcgcagcttg
    28381 aagaggccat cgcccaactg gattgtgaag acggcattgt ttttctgacg gatttgctgg
    28441 gcggcacgcc gtttcgcgta gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga
    28501 ttaccggcac caatttgcaa ctgctactgg agatggtgct ggagcgcgaa gggttaagcg
    28561 gtgaagaatt ccgtgtacag gcgctggagt gtggacaccg agggctgacc agtctggtgg
    28621 acgagttagg ccgctgccat gaagaatgtc cggtcgagga aggaatatga cacacgttct
    28681 gcgcgccaga aggctgctga ctgaagaggg atggttcgat gaccatcagt tgcgtgttgc
    28741 tgacggtgtc atcgcagcaa tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga
    28801 actgctctgc cccgcttaca tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat
    28861 ggatgacgcg ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg
    28921 cagttggtta ccgaccaccg taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg
    28981 tattgctcaa cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga gttatctcga
    29041 aggaccgtac ttcacgccgc agaataaagg cgcgcatcc
//
LOCUS       DAJTNW010000051        29049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNW010000051 DAJTNW010000000
VERSION     DAJTNW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO179_002294"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO179_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5532330.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3188
                     /gene="mdtO"
                     /locus_tag="OO179_002295"
     CDS             1140..3188
                     /gene="mdtO"
                     /locus_tag="OO179_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5532331.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3185..4651
                     /gene="mdtP"
                     /locus_tag="OO179_002296"
     CDS             3185..4651
                     /gene="mdtP"
                     /locus_tag="OO179_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5532332.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4849..5088
                     /locus_tag="OO179_002297"
     CDS             4849..5088
                     /locus_tag="OO179_002297"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532333.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5098..5268
                     /locus_tag="OO179_002298"
     CDS             5098..5268
                     /locus_tag="OO179_002298"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5532334.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5317..6729
                     /gene="fdhF"
                     /locus_tag="OO179_002299"
     CDS             5317..6729
                     /gene="fdhF"
                     /locus_tag="OO179_002299"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5532335.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6730..6996
                     /locus_tag="OO179_002300"
     CDS             6730..6996
                     /locus_tag="OO179_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5532336.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7090..7779
                     /locus_tag="OO179_002301"
     CDS             7090..7779
                     /locus_tag="OO179_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5532337.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO179_002302"
     CDS             complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO179_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5532338.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO179_002303"
     CDS             complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO179_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5532339.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO179_002304"
     CDS             complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO179_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5532340.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10481..12199)
                     /locus_tag="OO179_002305"
     CDS             complement(10481..12199)
                     /locus_tag="OO179_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5532341.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO179_002306"
     CDS             complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO179_002306"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5532342.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO179_002307"
     CDS             complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO179_002307"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5532343.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO179_002308"
     CDS             complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO179_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5532344.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO179_002309"
     CDS             complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO179_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5532345.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16280..18238
                     /gene="acs"
                     /locus_tag="OO179_002310"
     CDS             16280..18238
                     /gene="acs"
                     /locus_tag="OO179_002310"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5532346.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18438..18752
                     /locus_tag="OO179_002311"
     CDS             18438..18752
                     /locus_tag="OO179_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5532347.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18749..20398
                     /gene="actP"
                     /locus_tag="OO179_002312"
     CDS             18749..20398
                     /gene="actP"
                     /locus_tag="OO179_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5532348.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20576..21868
                     /locus_tag="OO179_002313"
     CDS             20576..21868
                     /locus_tag="OO179_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5532349.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22022..23671)
                     /locus_tag="OO179_002314"
     CDS             complement(22022..23671)
                     /locus_tag="OO179_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5532350.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO179_002315"
                     /gene_synonym="yjcD"
     CDS             complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO179_002315"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5532351.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO179_002316"
     CDS             complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO179_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5532352.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26427..26750
                     /gene="soxS"
                     /locus_tag="OO179_002317"
     CDS             26427..26750
                     /gene="soxS"
                     /locus_tag="OO179_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5532353.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO179_002318"
                     /pseudo
     CDS             complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO179_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28759..29040
                     /locus_tag="OO179_002319"
     CDS             28759..29040
                     /locus_tag="OO179_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5532354.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7778 c         7349 g         6634 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gctcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtatcggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     1381 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     1441 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     1501 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccatttac gggcaaacct
     1561 tccccgccat gctcgactat ccggaagtag tcgtgcgctt aacgctgtgg tgtatcgttg
     1621 ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt cccaatcgtg
     1681 ccattacgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
     1741 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
     1801 aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
     1861 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
     1921 cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcttca gaaatcaaca
     1981 agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
     2041 gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
     2101 tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
     2161 ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg ctcgcctgtt
     2221 tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc tgtatgctga
     2281 cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg gtgctgcgtt
     2341 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
     2401 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttggccgcat
     2461 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
     2521 cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa attcgcgatc
     2581 gcgccctggg tatcatcatt ggtaccgtgg tgtccgcgat gatttacacc tttgtctggc
     2641 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgttaagta
     2701 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
     2761 gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
     2821 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
     2881 gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg caggcgctgg
     2941 ataacgcact acagccggac agagcaggtc agtttgccga cgccctggag aaatacgctg
     3001 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccacct
     3061 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
     3121 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
     3181 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
     3241 gctgatttcc ggctgtgccc tggtacgtaa ggattctgcg cctcatcaac agctcaaacc
     3301 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
     3361 gtggtggaaa caactcaatg acccgcagtt ggatgccctg atccaacgga cgctaagtgg
     3421 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcaatcgc aggccgattt
     3481 gttagatgcc ggttcacagt tgcaggtggc agcgttaggg atgcttaacc gccaacgtgt
     3541 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg ggatggacgg
     3601 gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg atttgtgggg
     3661 tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
     3721 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
     3781 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
     3841 agcgcaccaa agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc acggcgcgcg
     3901 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa tcaccgaaac
     3961 gagagaatct ctgcgcgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
     4021 tgtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg agttgctcgc
     4081 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
     4141 ttccgcgcgg gcgttgttct atccgagctt tgatatcaaa gcgtttttcg gccttgatgc
     4201 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
     4261 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca cgcgcgccgc
     4321 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
     4381 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacacgt
     4441 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
     4501 ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt cattactgat
     4561 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcggcgggta
     4621 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg ctgtcacgcg
     4681 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
     4741 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
     4801 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
     4861 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
     4921 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
     4981 ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
     5041 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctgaga tgaggcactg
     5101 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
     5161 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
     5221 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
     5281 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
     5341 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
     5401 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
     5461 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
     5521 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcgttc
     5581 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaagg ctacacgccg
     5641 gagtcggttg aagatatcac cggtgtcagc gccagtgaga ttcgtcaggc ggcacggatg
     5701 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
     5761 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccagtaa cctcggtaag
     5821 ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
     5881 ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc taaccgcgaa
     5941 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggcta tcgcatcagc
     6001 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
     6061 ccgctgcaaa ctgatgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
     6121 gtcatcgttc aggacatctt tataaccaaa accgcgtcgg cggcggatgt cattttgccg
     6181 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
     6241 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
     6301 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
     6361 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
     6421 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
     6481 aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
     6541 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
     6601 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
     6661 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga agatgaggca
     6721 ttggtttgag tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
     6781 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
     6841 gttaccgaaa gcttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
     6901 gtcgagccga tctccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
     6961 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
     7021 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
     7081 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcg
     7141 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgtgca
     7201 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
     7261 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
     7321 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
     7381 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
     7441 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
     7501 aatgactccg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
     7561 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
     7621 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgagga aggttttatc
     7681 gagaagaaca aacaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
     7741 accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga taagacgcgg
     7801 caagcgtcgc atcaggcatc cacacattgc cgggggatat ccccggcaat cttcaattat
     7861 tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag cgcctttttg
     7921 cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt acccaccacg
     7981 ttcagcgcag tacgcgccat gtcgaggata cggtcaacac cagcaataaa cgccagacct
     8041 tccagcggaa tacctacgct acccagcgtt gccagcaaca ccacaaacga cacgccagcc
     8101 acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat ttcctgccag
     8161 atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact ttgatacagc
     8221 gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaagctggt gatcgacgcc
     8281 ggtgctccgt aggcttccat cttctcaata attcgcggca gtacgctttc agagctggca
     8341 gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca gacgcttaac
     8401 ccgcacagac gtgccacaat tcccagcact accagcgcga agaacagaat ggcgaaatgc
     8461 accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt agccaccgtc
     8521 accgcaatca gcgcaaacac acccaccggt gcataacgca tcaccatgtg agtcacttta
     8581 aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc
     8641 agggaagaaa gccccagacc aaacagcacc gagaaaaaga tgatcggcag catttcgcct
     8701 ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc cataatgccg
     8761 tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc
     8821 agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt gatccccaaa
     8881 atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc aatacgcccg
     8941 agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat cacaatcggc
     9001 acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag caaattgacg
     9061 accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag aatgcccagc
     9121 accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat cagaataact
     9181 tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac tgccagggca
     9241 tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta tcatttgcgc
     9301 ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca taataacgcg
     9361 agtttatggc aggattaatg aataaccatt tattatggaa agatttttat ttgtttaatc
     9421 tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat tttttatgca
     9481 gctcaatgac ataatcttca tcaccagatg attaatccga tctccgctgc aacagttttg
     9541 ccatattaat cgactcgacc agctgtgtcc ggttgatccg gggtgagttg agatccatca
     9601 ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca tcggaagcca
     9661 gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc
     9721 gagtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc agcgccgcat
     9781 acagttcagc gttctcacca cgtagttgca acgcctgacg atacgccagc agcgaattgc
     9841 tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccattgttcg ctgttttgtg
     9901 gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggggtt tgctggctgg
     9961 caaactgatg tagcggatcg cgctgacgct gatactccgc acgtaccgcc tgccattttg
    10021 ggcttaacag ataactgccg acacagagga ataccatcaa tatcgtgagt gttgtcagca
    10081 ttttcaccgg tatttgaggc tgtttcatcg cttagccctc actcgccaga ggatcagtgc
    10141 catcagcaat aacaacacca ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg
    10201 tgggttatag cgaacaaaat ctccgtagcg ttcggtcatc cagccaatga tttcgacttc
    10261 gttcttcccc tccgacacca tgctataaac ctgatggcgc atactgaccg ccaccggcgc
    10321 attggattcg agtaagtttt gattctggca ctgcggacaa cgtaactggc tggcaatatt
    10381 taacgcctgt tgctgttgtt gcggattggc gaattgccag gtgtctacaa cctgagcgcg
    10441 cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg cgcttcttcc
    10501 cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc
    10561 gcacaccgct ttgtacatac aaacgaaaag cgtaacgatc cgctctggtt ttttcaccca
    10621 tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc atttgctgac
    10681 ggcgtgcttc ataaaaacgc cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa
    10741 acagcgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga tcgagtcgct
    10801 caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa ttcaggctga
    10861 tctcctgacg actgacactg gaaaccacga tccccgcggc aaataacagc acgccagcat
    10921 gagctaccag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc agcacaatca
    10981 ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc gcgccaacgg
    11041 agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc accagcacga
    11101 tcagtagcac tgcgcaaaac agcaacagcg tagcgaggat taacatttcc cgcgataacc
    11161 ccgaaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga gccaaagacg
    11221 caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta tccagtgcga
    11281 acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtgcccagc agcgacagca
    11341 tcagagtaac tatcgccagc aacagcgacc agtggcggaa aatcccccgc tggcgtgtca
    11401 gggacaaact gtgcagcagc gcagtggcag aaagccaggg taataaagag gcgttttcca
    11461 ccggatccca gaaccaccag ccgccccagc cgagttcgca gtaggcccac caggaaccga
    11521 ggatgatccc cgccgttaat gcactccagc caggtaacgc ccagcgccag caaattcggg
    11581 cgctggccgc atcaaactcg ccgcgcagta gactcgccag cgccacgctc gccgctacca
    11641 tcaaaccgcc atagccaagg caaagcagcg gtggatgaaa gataagaccg ggatgttgca
    11701 gcatcggatt gagatcgcgg ccttcgattg ctggtggaaa tatccgcacg aagggatcgg
    11761 accacagcac cacaaacagt agcagtgcgg cgagcatgag agataaaacg gctagcgtca
    11821 gcggaaatag cggatcgctt tgctgccgat aatgccaggc aaacagcgcg ctccaggcgg
    11881 aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc gccgccagtt
    11941 tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc gagaaatcgc
    12001 tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg aactgcgcca
    12061 gaatgccgat gcaagtgagt cgcatcatgg caggccagcg caaccgcact cccgcaaagg
    12121 ccgtcaacgg ggtcaacacg ttgaccccga gacttaacaa caacgccaga aaaccgactt
    12181 caggaaggaa tacgtccaga aaccacctct tttctggctt acgccaccgt caactgtccg
    12241 gcgtagagaa tgaaaaagcg cagcatcaac acgccgacca gactggcccc acacgccgcc
    12301 agcacggcag gaatgccgga actgcgattg acccacggtt tgagcaacat tggcacaatc
    12361 agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc acctaatgcc
    12421 gccaccagcg cacgaacttt accgtcatcg cccagcgcca gaccgacaaa aaacgccacc
    12481 agcaggaaga tttcacccca taccacgggg atttccatcc ggtgaacaaa gtgcgcttcc
    12541 gtggaatgcg ggttactgcg ttggcgtatc gccatggcga tcagcgccac cgccgcaccg
    12601 gacgagatgc cggagaagag gagcagcacc ggcaggctcg ggttattgag gaacggatac
    12661 gatttcagcg ccgacagcag aaagccggta taagccccca gcagcaccgc caacaccagc
    12721 atcagcgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc
    12781 cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc
    12841 agccacagca ccagcaccac catgtagagc tgaaacagca tcacccccat cgacatcacc
    12901 gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt caggtggaag
    12961 acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt ggtgcgcagc
    13021 aacgtactgt ctgcaccgcc cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc
    13081 accagaccgg cagaaatacc aatcaaaaac aggtagatgg caatcggcca gtcccacacc
    13141 agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg tatttaaacg
    13201 gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa gtaggcttct
    13261 ggcgcagcag ttgcgaaatc tcactgttag gatcgtccag attgccaaac gtcagcgcct
    13321 tcgtcgggca agcttcaacg cacgcgggaa gcttaccggc ctgcaaattg gtcttacgac
    13381 agaaatcgca tttgtccgcc gtcttcgtga ccggatggat aaagcgcacg cggtacggac
    13441 aggcggcgat gcagtactga caaccgacgc agagatcagg attaacatcg acaatcccgc
    13501 tagcggcatc acgaaaagac gcaccggtcg ggcagacgtc aacgcacggc acatgatcgc
    13561 agtgctggca agacttacgg aagaagcgat atttcacatc aggaaattcg ccctgcggct
    13621 cgctacgaat aatcgtcagg cgcgagacgc cttccggcac cttgttcact tcccgacaag
    13681 catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg ccgtaacgca
    13741 ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg acggctgcca
    13801 gcacgccgac gccggtgaga aactggcgac gagaccaggt catggctgct ccttaagcaa
    13861 cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc aatcgacgca
    13921 aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga
    13981 atggcagctg gcgcacgcca ctttggttac gtggacatcg tgcggccaga acgctttttg
    14041 caactgttcc ggcagatgac aggacataca gacgctgttc tgctctccga ccttatacat
    14101 cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg atgcttggcc
    14161 gtgacagttg gtgcaggtga ccggcaattt gttattcggg ttgataacgg aagcatgttt
    14221 gccatgcata ccttcggtat ctggtttatg acagtccaga caggcggcat ccgggttacg
    14281 ctgctgggta acttcgtagc gatcaccaga tgcctgcacc gcaggggtag cggtaattcc
    14341 gttcaggctc cacaacaggc ccgacgccag caccccagca gttaataacg aacgtaatac
    14401 gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg ttattggctt
    14461 aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc
    14521 tgcttctcgg ccttgatttg ttccatgttc aggccaatgg cctgctgggc tttctctttg
    14581 gtcgagatat ccgggatctg gatttcatgg gtgatacctt tggtcgccag caggcgcgcc
    14641 agtttggtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatccg tagaccttct
    14701 tccggtgcgt gcatatgaat accgtgggaa gcgatcgcca gatcccaacg ccactgggca
    14761 tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc atccagcgcc
    14821 gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta
    14881 atcgattgct tacgttccgc gaccactttt tgcaaggcag ctttatcctg ggtatggcag
    14941 ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc ggtgtagagt
    15001 ttgccttcgg cgttctgcac ttttggcata tggcagtcga tacaggtcac gttgttttta
    15061 ccgtgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag cattggcgtt
    15121 ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg ctccatattt
    15181 tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa
    15241 tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc ggctttctca
    15301 aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag ggttaactcc
    15361 ggtttgcctt tggcgaactc tggtgaggcg gtgttatggc agtcggcaca acctaagttg
    15421 ttgacgattt ccgggccgcc gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct
    15481 ttctggatca gacgcgccac atccgggctt ttacaactcc agcacgccat cggtagcgga
    15541 ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc ggtcacagca
    15601 aaggcatgtc cacgaggctt gttgtaatcg cgcgagaagg gataccccgc ccacaggatc
    15661 accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga ggtggctttc
    15721 caggagagat attgatcgtg atgctgcggg gcaaaggttt cattcttcgc ttccacagtt
    15781 acaggttttg cgggagctgc cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga
    15841 atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc
    15901 gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta accgcaccta
    15961 cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg aatgttgtct
    16021 ttaatcaatt gtaagtgcat gtgaaatacc actttagagt tagtcagtat cttcctcttt
    16081 ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg ttggtgacct
    16141 tcaaaaatta ccctgccgtt tatttgcacc attctacttt tgcgtgatct gtcgcccaaa
    16201 tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat tattaacatc
    16261 ctacaaggag aacaaaagca tgagccaaat tcacaaacac accattcctg ccaacatcgc
    16321 agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat ctattaacgt
    16381 acctgatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac cgtaccagaa
    16441 ggtgaaaaat acctcctttg cccccggtaa tgtgtccatt aaatggtacg aggacggcac
    16501 gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg atcgcaccgc
    16561 catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata aagagctgca
    16621 ccgcgacgtc tgccgcttcg ccaataccct gctcgagctg ggcattaaaa aaggtgatgt
    16681 ggtggcgatt tatatgccga tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg
    16741 cattggcgcg atgcattcgg tgattttcgg cggcttctcg ccggaagccg ttgccgggcg
    16801 cattattgat tccaactcac gactggtgat cacttccgac gaaggcgtgc gcgccgggcg
    16861 tagtattccg ctgaagaaaa acgttgatga cgcactaaaa aacccgaacg tcaccagcgt
    16921 agagcatgtg gtggtactga agcgtactgg cggaaacatc gactggcagg aagggcgcga
    16981 cctgtggtgg cacgacctgg ttgagcaagc cagcgatcag caccaggcgg aagagatgaa
    17041 cgccgaagat ccgctgttta ttctctatac ctccggttct accggaaaac caaaaggcgt
    17101 actgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat atgtctttga
    17161 ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta ccggacacag
    17221 ttatttgctg tacggcccgc tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc
    17281 gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg tcaatattct
    17341 ctataccgca cccacggcga tccgcgcgct gatggcggaa ggcgataaag cgattgaagg
    17401 caccgaccgt tcgtcgctac gcattctcgg ttccgtgggc gagccaatta acccggaagc
    17461 gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg
    17521 gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg agctgaaagc
    17581 cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa
    17641 cccgctggaa ggggctaccg aaggtagcct ggtgatcacc gactcctggc ctggtcaggc
    17701 gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tatttttcca ccttcaaaaa
    17761 tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact ggatcaccgg
    17821 gcgtgtggac gacgtgctga acgtctccgg tcaccgtctg gggacggcag agatagagtc
    17881 ggcgctggtg gcgcatccga agattgccga agccgccgta gtaggtattc cgcacaatat
    17941 taaaggtcag gcgatctacg cctatgtcac gcttaatcac ggggaggaac cgtcaccaga
    18001 actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg cgacgccaga
    18061 cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat
    18121 tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga cgcttgccga
    18181 tcctggcgtg gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc
    18241 cacaattgcc ggatgcgaat cggtaacggt ttgtaggcct gataagacgc gacagcgtcg
    18301 catcaggcat tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaagg
    18361 gttacccgag cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta
    18421 cctctggaga atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt
    18481 tcagggagtt agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg
    18541 cagtttatat cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcacgccgc
    18601 tgaatccgaa caccagcgtc acacgcggta ttccaattgg tgttggagtg attgtgatct
    18661 cctttgttct caccggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata
    18721 atgaagtcct gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac
    18781 actccctttc gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac
    18841 gaactggcag gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta
    18901 ctgggcatca aaacgcgtac gttctcgtag cgactactac accgcaggcg gcaatatcac
    18961 tggcttccag aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg
    19021 gatctccgcg ctggtgttta cctccggcta tgacgggctg atctactcgt tgggcttcct
    19081 ggtgggctgg ccaatcattc tgtttctgat tgccgaacgt ctgcgtaacc tgggacgcta
    19141 cacctctgcc gatgtggcct cttatcgtct gaaacaaggg ccgattcgta ttctttcggc
    19201 ctgtggttct ctggtagtgg tggcgcttta ccttatcgct cagatggtag gcgcaggtaa
    19261 actgatcgag ctgctgtttg gccttaacta tcacattgcg gtggtgctgg tcggcgtgct
    19321 gatgatgatg tacgtcctgt tcggcggcat gctggcgacc acctgggtac aaattatcaa
    19381 agctgtgctg ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg
    19441 ctttagcttc aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat
    19501 catgaaaccg ggcggactgg tgaaagatcc gatctccgcg ctctctctgg ggctgggact
    19561 gatgtttggt acggcgggct tgccgcacat tctgatgcgc ttctttacag tcagcgatgc
    19621 ccgcgaagca cgtaagagcg tgttctacgc caccggattt atgggctact tctatattct
    19681 gacctttatt atcggcttcg gcgcgatcat gcttgttggt gcgaatccgg aatataaaga
    19741 cgcggcgggc catctgattg gtggtaacaa catggcggcc gttcacctgg cgaatgcagt
    19801 gggcggcaac ctattcctcg gttttatttc agcggttgct ttcgccacta tcctcgcggt
    19861 ggttgcggat ctgacactgg cgggcgcatc ggcggtttcg catgacttgt acgctaacgt
    19921 cttcaaaaaa ggcgcgaccg aacgtgaaga gctgcgggta tcaaaaatca ccgtactgat
    19981 cctcggcgtg attgcgatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat
    20041 ggtaggcctt gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat
    20101 gtactggtcg aaactgacta cccgtggcgc gatgctgggc ggctggctgg ggctgattac
    20161 cgcagtggtg ctgatgatcc tcggcccgac tatttgggta cagatcctcg gtcacgaaaa
    20221 agccatcttc ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggcat
    20281 ctggttcttc tcggcaaccg ataactcagc ggaaggggcg cgcgagcgtg aactgttccg
    20341 cgcgcagttt atccgctccc agaccggctt tggcgttgag caaggccgcg cacattaatc
    20401 tctccccttc cccggtcgcc tgaccgggga atacgcttcc tctccaacat gcatcacctt
    20461 ttcccataat attaaacaaa taaactcatt aaaaaatgag cgatttttga cagtcgtaga
    20521 aaatgataat gcagagaata tgccttttct ttcttgttaa ttataaggat attttatgcg
    20581 ctataatggt ttaaataata tgtttttccc tctttgccag attaacgata accactctat
    20641 cacaagttca tcacatacaa agaaaacaaa atcagataat tacagcaaac atcataaaaa
    20701 cacgttaatt gacaataaag ccctctctct tttcaaaata gatgatcatg aaaaagtgat
    20761 aggcttgatt cagaaaatga aaagaattta tgatagttta ccatcaggaa aaatcacgaa
    20821 agaaacggac aggaaaatac ataaatattt tatagatata gcgttatatg caaataataa
    20881 atgtgacgat agaattacga gaagagttta ccttagtaaa gaaaaggatg tatccattaa
    20941 ggtggtatat tttataaata atgtcgccgt ccataataat actatcgaaa ttccacagat
    21001 agtaaatggt ggttacgatt tttcacacct tagcctgaag ggtatcgtga ttaaagatga
    21061 agatttatcc aattcgaatt ttgcaggttg cagactacaa aacgctattt tccaggactg
    21121 taatatgtat aaaacgaatt tttattacgc cataatggaa aaaatacttt ttgataattg
    21181 tattctcgat gactcaaatt tcgctcagat aaaaatggcc gacggaactc taaatgcatg
    21241 ctccgctatg catgttcaat tctacaatgc agcaatgaat agagccaata ttaaaaatac
    21301 cttccttgac tattcaaatt tttatatggc gtacatggct gaggtaaatc tttataaagt
    21361 aatagcgcca tatgttaatt tatttaaagc cgaccttagt ttctctaaac tcgatttaat
    21421 taactttgaa catgctgatc tgtctcgcgt caatctgaac aaagcaatcc tccagaatat
    21481 aaacttaatt gatagcaaac tcttttgtac gtggctgaca aatacattcc tcgaaatggt
    21541 tatatgtacc ggctctaata tggctaatgt taattttaat aatgccaatt taagcaactg
    21601 ccatttcaac tgttctattt taacaaaagc ctgtatgttt aatacccgtc tctatcgggt
    21661 taatttcgat gaggctagcg tccagggaat gggcatttcc attctccgtg gggaggaaaa
    21721 tatccccatt gatagtgata ccctggtaac actacagaaa ttctttgaag aagattgtac
    21781 ctctcatact ggcatgtcac aaactgagga taatattaat gcagtcgcta tgaagattac
    21841 tgcagatatt atgcaacacg cagattgagg ttaagtcgaa gaacagccaa tgaattgact
    21901 ttacaagaga gataacttat ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt
    21961 aaaggtgcaa aagattgcac ctttttcaga ttacctcggg cctggttacg tcgctttcgg
    22021 cttactgatt ttcctcaatt agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    22081 gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    22141 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    22201 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctgacctccg
    22261 taagcaactg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    22321 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttct tctttcaatt
    22381 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    22441 caaacagcga gaagagaatg acgccagccg ccaggaacac cagctcatag cgcgccggga
    22501 agacgttacc atccggcaag agcagcggga ttgagagcac accggccaga gtgatcgccc
    22561 cacgcacccc agcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    22621 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    22681 ggaccagcat cagcgccgca tatatcagaa taatatcggt aaacagcatc cagatttcga
    22741 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agttgcagac
    22801 ctaacagcag gaacaccatg ccgttaaaga caaattccag catcgcccag gtgctgtttg
    22861 cgcgcaggcg cattgccagt ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc
    22921 cggcggcaac cgcagcgagg atgcccgaaa cgccaatatg ttcggcaatc agataagaag
    22981 cgaatggcag caagaacagc agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    23041 tgaggaatcg cagcgagcga ccatacagcc agctcaccac aaaaccggca agaataccgc
    23101 caatggctac tttcataaat tcgaccgtcg caccgccgac agtgaagatc atcgtcccca
    23161 tcgccactgc cacggcaaac ttcaacgaca ccaggccgga agcgtcattc atcaacgctt
    23221 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atcccggaga
    23281 gcgccacagc atcggtcgga gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa
    23341 tgcccggcac cacccagtaa ataaggaagc caatgccgac cacggtgacc accaccagcg
    23401 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    23461 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    23521 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    23581 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    23641 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    23701 tctgagaaag acgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    23761 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat ccgatgaaag
    23821 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacggcgata acgaccgtac
    23881 cgatgttcaa cttgcgccat tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    23941 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    24001 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttgctc agcatcagca
    24061 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gaaagcggag
    24121 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaac ccggttttac
    24181 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    24241 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    24301 gcccatcttt atccagcagg ttcgcctggc cggcgacagc gcggatagtt ccggtggcat
    24361 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    24421 ccataatgtc caggctgcca atcagtgaat tgccgttttc atcgctcaat gaaggcatgg
    24481 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    24541 taatggtcag cagaatgcca ccagggactt tcagtttttc caagccgatg atcaccgcca
    24601 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
    24661 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
    24721 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    24781 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
    24841 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
    24901 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    24961 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
    25021 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
    25081 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    25141 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    25201 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    25261 tgcggcaatt tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    25321 ggtaaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
    25381 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
    25441 tcgtcgatgt gcaagattgc agcgttatcg agccgtatac cgttctggat tgcgataaaa
    25501 aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac tggcgaagaa
    25561 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
    25621 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgttcca gcgggataga gagaaagaca
    25681 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgtgtt tgcggataat
    25741 tccaaaagag ctcgatcagt cccctcgccc cttcggggag agggttaggg ttaggggaac
    25801 aggcagcacc agtgcaaata tttaccctca ccccggcccc atagttaacg atgagtcagc
    25861 ttgtccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
    25921 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
    25981 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
    26041 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttctg
    26101 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcgattt
    26161 tgataattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
    26221 ggatactagt aatcaaccct ttgctttcat agaaatgcag cgccgatacc gccacaccgc
    26281 tgcgtttcgc cacttcgcca ggggttagca gcgctttaat gcggggtaat ttcttttcca
    26341 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
    26401 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
    26461 catggattga cgtgcatatt gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag
    26521 gctattcaaa gtggtacttg caacggatgt tccgcacggt gacgcatcag acgcttggcg
    26581 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
    26641 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
    26701 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag ttttattgcc
    26761 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca
    26821 tcgccttcgc aaaaaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
    26881 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
    26941 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
    27001 atttatcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
    27061 caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc agaattaatc
    27121 ggcgtcattt tttcgacatc aagaaatgca tgctcagtca cttcaaattt aatttgctgc
    27181 ggacgcaccg cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga
    27241 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
    27301 cccagatcgc ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc
    27361 ttttctgcca gcggaataaa ctctgccgga ttcattattt gcccctgctc accaggccaa
    27421 cgtaacaacg cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa
    27481 taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag
    27541 ttttgtcgaa tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc
    27601 aacgcaaaaa taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca
    27661 atggctgcaa ttgggcgttt tgtcgaataa attgtcgcat ataaatagcc atttctttgt
    27721 acggttaaat ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt
    27781 aacgagaaaa aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca
    27841 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
    27901 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
    27961 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
    28021 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
    28081 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
    28141 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
    28201 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
    28261 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
    28321 gatgactcat tgctatcaat ttcctgcctg tttataccca gccattgcaa ggtgaaaccc
    28381 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
    28441 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
    28501 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
    28561 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
    28621 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
    28681 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
    28741 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
    28801 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
    28861 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
    28921 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca caacatcgag
    28981 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttgacctga
    29041 tacgtagaa
//
LOCUS       DAJTNW010000052        29002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNW010000052 DAJTNW010000000
VERSION     DAJTNW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..218
                     /locus_tag="OO179_002320"
     CDS             <1..218
                     /locus_tag="OO179_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532355.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO179_002321"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO179_002321"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5532356.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO179_002322"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO179_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5532357.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="OO179_002323"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="OO179_002323"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5532358.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="OO179_002324"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="OO179_002324"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5532359.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="OO179_002325"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="OO179_002325"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5532360.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="OO179_002326"
     CDS             7229..8179
                     /locus_tag="OO179_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5532361.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="OO179_002327"
     CDS             complement(8220..8690)
                     /locus_tag="OO179_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5532362.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="OO179_002328"
     CDS             complement(8745..9947)
                     /locus_tag="OO179_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5532363.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="OO179_002329"
     CDS             complement(10034..10576)
                     /locus_tag="OO179_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532364.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="OO179_002330"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="OO179_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5532365.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="OO179_002331"
     CDS             complement(11319..11921)
                     /locus_tag="OO179_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5532366.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="OO179_002332"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="OO179_002332"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5532367.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="OO179_002333"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="OO179_002333"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5532368.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="OO179_002334"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="OO179_002334"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5532369.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="OO179_002335"
     CDS             16319..17209
                     /locus_tag="OO179_002335"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5532370.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="OO179_002336"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="OO179_002336"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5532371.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="OO179_002337"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="OO179_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5532372.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="OO179_002338"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="OO179_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5532373.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO179_002339"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO179_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5532374.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO179_002340"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO179_002340"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5532375.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO179_002341"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO179_002341"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5532376.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO179_002342"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO179_002342"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5532377.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO179_002343"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO179_002343"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5532378.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO179_002344"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO179_002344"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5532379.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO179_002345"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO179_002345"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5532380.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO179_002346"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO179_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5532381.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="OO179_002347"
     CDS             complement(27384..27845)
                     /locus_tag="OO179_002347"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5532382.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="OO179_002348"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="OO179_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5532383.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7006 a         7821 c         7250 g         6925 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactagtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca ac
//
LOCUS       DAJTNW010000053        28492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNW010000053 DAJTNW010000000
VERSION     DAJTNW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(92..229)
                     /gene="yjdI"
                     /locus_tag="OO179_002349"
                     /pseudo
     CDS             complement(92..229)
                     /gene="yjdI"
                     /locus_tag="OO179_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
     gene            410..2041
                     /gene="dcuS"
                     /locus_tag="OO179_002350"
     CDS             410..2041
                     /gene="dcuS"
                     /locus_tag="OO179_002350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5532384.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2038..2757
                     /gene="dcuR"
                     /locus_tag="OO179_002351"
     CDS             2038..2757
                     /gene="dcuR"
                     /locus_tag="OO179_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5532385.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3328..4668
                     /gene="dcuB"
                     /locus_tag="OO179_002352"
     CDS             3328..4668
                     /gene="dcuB"
                     /locus_tag="OO179_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5532386.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4746..6392
                     /gene="fumB"
                     /locus_tag="OO179_002353"
     CDS             4746..6392
                     /gene="fumB"
                     /locus_tag="OO179_002353"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5532387.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6515..7144
                     /locus_tag="OO179_002354"
     CDS             6515..7144
                     /locus_tag="OO179_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5532388.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7283..8704)
                     /gene="melB"
                     /locus_tag="OO179_002355"
     CDS             complement(7283..8704)
                     /gene="melB"
                     /locus_tag="OO179_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5532389.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8819..10162)
                     /gene="melA"
                     /locus_tag="OO179_002356"
     CDS             complement(8819..10162)
                     /gene="melA"
                     /locus_tag="OO179_002356"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5532390.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10445..11353
                     /gene="melR"
                     /locus_tag="OO179_002357"
     CDS             10445..11353
                     /gene="melR"
                     /locus_tag="OO179_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5532391.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11552..13819
                     /gene="adiA"
                     /locus_tag="OO179_002358"
     CDS             11552..13819
                     /gene="adiA"
                     /locus_tag="OO179_002358"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5532392.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14144..14905
                     /gene="adiY"
                     /locus_tag="OO179_002359"
     CDS             14144..14905
                     /gene="adiY"
                     /locus_tag="OO179_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5532393.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            15042..16379
                     /gene="adiC"
                     /locus_tag="OO179_002360"
     CDS             15042..16379
                     /gene="adiC"
                     /locus_tag="OO179_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5532394.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16483..18126
                     /gene="eptA"
                     /locus_tag="OO179_002361"
     CDS             16483..18126
                     /gene="eptA"
                     /locus_tag="OO179_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5532395.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18123..18791
                     /gene="basR"
                     /locus_tag="OO179_002362"
     CDS             18123..18791
                     /gene="basR"
                     /locus_tag="OO179_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5532396.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18792..19892
                     /gene="pmrB"
                     /locus_tag="OO179_002363"
     CDS             18792..19892
                     /gene="pmrB"
                     /locus_tag="OO179_002363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5532397.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19868..19957)
                     /gene="pmrR"
                     /locus_tag="OO179_002364"
     CDS             complement(19868..19957)
                     /gene="pmrR"
                     /locus_tag="OO179_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5532398.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(20069..21571)
                     /gene="proP"
                     /locus_tag="OO179_002365"
     CDS             complement(20069..21571)
                     /gene="proP"
                     /locus_tag="OO179_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5532399.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21835..22713)
                     /locus_tag="OO179_002366"
     CDS             complement(21835..22713)
                     /locus_tag="OO179_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5532400.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22710..24938)
                     /gene="crfC"
                     /locus_tag="OO179_002367"
     CDS             complement(22710..24938)
                     /gene="crfC"
                     /locus_tag="OO179_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5532401.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25340..25675
                     /locus_tag="OO179_002368"
     CDS             25340..25675
                     /locus_tag="OO179_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5532402.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25833..26276
                     /gene="yjdN"
                     /locus_tag="OO179_002369"
     CDS             25833..26276
                     /gene="yjdN"
                     /locus_tag="OO179_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5532403.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26409..27197
                     /gene="phnC"
                     /locus_tag="OO179_002370"
     CDS             26409..27197
                     /gene="phnC"
                     /locus_tag="OO179_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5532404.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27222..28238
                     /gene="phnD"
                     /locus_tag="OO179_002371"
     CDS             27222..28238
                     /gene="phnD"
                     /locus_tag="OO179_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5532405.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28344..>28492
                     /locus_tag="OO179_002372"
     CDS             28344..>28492
                     /locus_tag="OO179_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5532406.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7324 a         7437 c         6953 g         6778 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttaaataagt gatactggtt
       61 gtctggagat tcagggggcc agtctacacg actttcgttt gagattaaat aacttgccgt
      121 tgccacgtac gcaattgcca gaatgctgac atatcgcagt gttgaaatag acatcgatct
      181 tttcgccggt ataacagcga taacccccgt ccagtagcgc ctgatccatg accttccctc
      241 tctcatcagt atgattatcg ctataagcat agccccggaa tcttcataca gcactggcag
      301 tttccgttgc cagagtctct tcccctatat agccaatcat ttttactccc tttgaattac
      361 ccgcctcatc agagataatg cttaagaaat ttgtcacaca aggaagctga tgagacattc
      421 attgccctac cacatattac gcaaacgtcc gatgaaattg agtaccactg tgatcttaat
      481 ggtcagcgcg gtactgttct cggtgctatt ggtggtgcat ctgatttact tctcgcaaat
      541 cagtgatatg acgcgagacg ggctggccaa caaggcactg gcagtggcgc gtaccctcgc
      601 cgactcgccg gaaatccgtc agggcttgca gaaaaaaccg caggagagtg gcatccaggc
      661 catcgcggaa gccgtgcgca aacgcaacga tctgctgttt attgtcgtta ccgatatgca
      721 aagtcttcgc tactcacatc ctgaagccca gcgtattggt cagccattta aaggtgatga
      781 catccttaat gcgctgaatg gcgaagaaaa tgtcgctatc aatcgcggtt ttctggcgca
      841 ggctttacgc gtatttaccc ccatctacga tgaaaatcat aaacaaattg gcgtggtggc
      901 gatcggcctt gagttaagcc gcgtgaccca acagatcaat gacagtcgct ggagcattat
      961 ctggtcggta ttatttggca tgctggtcgg actgattggc acctgcattc tggttaaggt
     1021 actgaaaaaa atccttttcg gcctggaacc ctacgaaatc tccacgctgt ttgagcaacg
     1081 ccaggccatg ttgcagtcta tcaaagaagg cgtcgttgcc gtggacgatc gcggcgaggt
     1141 cacgctgatc aacgatgccg cacaagaatt gctgaattac cgtaagtcgc aggacgatga
     1201 gaaactgtcg acgctcagcc actcatggtc acaggtggta gatgtctcgg aagtgttacg
     1261 cgacggtacg ccgcgccgcg acgaagagat tacgattaaa gaccggctat tactgatcaa
     1321 caccgttccg gtgcgcagta atggcgttat catcggtgcc atttcaacct tcagggacaa
     1381 aactgaagtt cgtaaactga tgcagcgact cgacggtctg gtcaactatg ctgacgcact
     1441 tcgtgaacga tcccacgaat ttatgaataa attgcatgtg attctcggat tattgcatct
     1501 gaagagttat aagcagttgg aagattacat tctcaaaaca gccaataact atcaggaaga
     1561 gattggctct ctgctgggta agatcaaatc tccggttatc gctggttttt taatcagcaa
     1621 gattaaccgc gcgaccgatt taggccatac gctgatttta aacagtgaaa gccagctgcc
     1681 agacagcggc agtgaggacc aggtcgcgac gctgattacc acattgggaa atctgataga
     1741 aaacgcgctg gaagcattag ggccggaacc cggtggcgaa attagcgtaa cattgcacta
     1801 ccgtcacggc tggctgcact gcgaagttaa cgatgatgga ccggggatcg cacccgacaa
     1861 aatcgatcac atttttgaca aaggtgtctc gacaaaagga agcgagcgag gcgtcggttt
     1921 agcacttgtc aaacaacagg tagaaaatct cggcggcagc atcgccgtgg aatcggaacc
     1981 ggggattttc acacaatttt ttgtccagat accctgggac ggggagaggt cgaacagatg
     2041 atcaatgtat taattatcga tgacgacgca atggtcgcgg agctgaatcg ccgatacgta
     2101 gcacaaatcc caggctttca atgctgtgga acagcctcga cgctggagaa agccaaagag
     2161 attatcttca atagcgatgc gcctatcgac ctgatattgc tcgatatcta tatgcaaaaa
     2221 gagaacgggc tcgatttact gcctgtcctg cataacgcgc gttgcaaaag tgatgtgatt
     2281 gtcatctcct ccgcagccga tgcggcaacc attaaagatt cgctgcatta cggtgtcgtg
     2341 gattacctga tcaaaccctt ccaggcttcc cgctttgaag aggcgctcac cggctggcgg
     2401 caaaagaaaa tggcgctgga aaaacatcag tattatgacc aggccgagct tgatcagcta
     2461 atccacggca gcagctccaa cgaacaggat cctcgtcgct tgccaaaagg cttaacgccg
     2521 cagacgttac gcacgctgtg ccagtggatt gacgcgcatc aggactatga gttttcaacc
     2581 gacgagctgg ctaacgaggt taacatctcg cgtgtttcct gccgaaaata cctcatctgg
     2641 ctggtcaact gccacatctt gttcaccagt atccattatg gcgtcacggg gcgtccggtt
     2701 taccgttacc gtattcaggc agaacactac tcactgctga aacaatattg ccaataacgg
     2761 atcgctggtt atctgtaagt aataattatt accgtaatgc aaaatacccc tggcacaagt
     2821 tacatcgtta atcagtaaaa cttgtgccag atcaaatata attatccctc catcatcgtt
     2881 aaaaattaat atctcttcag atgaacggtg tttttaattt caaaacgata acaaaagtta
     2941 attaactatt atgtcacccg cattatgtgt atttttaccc acaaatgggt agatcagatt
     3001 aatctataaa cctaatgata tctgccctga gaacaaaaaa tagaccgata aatatcaata
     3061 agataacagc aaacaaaaca ttaacatctg cgcagtacaa actataaacc tatcgccaga
     3121 gagtttttct ctctgaaaaa gccgcttatc acagtgcata aatttgccgc tgctttaatc
     3181 agccaatatt cactgtgagg tatttgctaa agccggtaac gaccaaacgg atatttagtc
     3241 aggctctgaa aacagttcat acaaaacaga acgtgactgt gatctattca gcaaaaattt
     3301 aaataggatt atcgcgaggg ttcacacatg ttatttacta tccaacttat cataatacta
     3361 atatgtctgt tttatggtgc cagaaagggt ggtatcgcgc tgggtttatt aggcggtatc
     3421 ggtctggtca ttctggtctt cgtcttccac cttcagccag gtaaaccacc agttgatgtc
     3481 atgctggtta tcattgcggt ggtggcggca tcggcgacct tgcaagcttc gggcggtctt
     3541 gatgtcatgc tgcaaattgc cgagaagctg ctgcgccgca acccgaaata tgtctcaatt
     3601 gtcgcgccgt ttgtgacctg tacactgacc attctttgcg gtacgggtca tgtggtttac
     3661 accattctgc cgatcatcta cgacgtcgcc attaagaaca acatccgtcc ggaacgtccg
     3721 atggcggcaa gttctatcgg tgcacagatg gggattatcg ccagtccggt gtcggttgcg
     3781 gtcgtgtctc tggttgcgat gctgggtaat gtcacctttg atggtcgcca tcttgagttc
     3841 ctcgatctgc tggcaatcac cattccatcg acgttaatcg gtatcctggc gatcggtatc
     3901 ttcagctggt tccgcggtaa agatctggat aaagacgaag agttccagaa attcatctcc
     3961 gtaccggaaa accgtgagta tgtttacggt gataccgcga cgctgctgga taaaaaactg
     4021 ccgaaaagca actggctggc aatgtggatt ttcctcgggg caatcgctgt agtcgccctt
     4081 cttggtgctg attcggacct gcgtccatcc ttcggcggca aaccgctgtc gatggtactg
     4141 gttattcaga tgtttatgct gctgaccggg gcgctgatta ttatcctgac caaaaccaat
     4201 cccgcgtcta tctcaaaaaa cgaagtcttc cgttccggta tgatcgccat cgtggcggtg
     4261 tacggtatcg catggatggc agaaaccatg ttcggtgcgc atatgtctga aattcagggc
     4321 gtactgggtg aaatggtgaa agagtatccg tgggcgtatg ccattgttct gctgctggtt
     4381 tccaagtttg taaactctca ggctgcggcg ctggcggcga ttgttccggt cgcgctggcg
     4441 atcggcgttg atccggcata catcgtggct tcagcaccgg cttgctacgg ttattacatc
     4501 ctgccgactt atccgagcga tctggcagcg attcagtttg accgttccgg caccacccac
     4561 atcggtcgct tcgtcatcaa ccacagtttt attctgccgg ggttgattgg tgtgagcgta
     4621 tcgtgcgtct tcggctggat cttcgccgcg atgtacgggt tcttataaat gcactttgcg
     4681 tgccgcccgt gactacgcgg cacgccattt tcgaataaca aatacagagt tacaggctgg
     4741 aagctatgtc aaacaaaccc tttatctacc aggcaccttt cccgatgggg aaagacaata
     4801 ccgaatacta tctactcact tccgattacg ttagcgttgc cgacttcgac ggcgaaacca
     4861 tcctgaaagt ggaaccagaa gcactgaccc tgctggcgca gcaagccttt cacgacgctt
     4921 ctttcatgct acgtccggca caccagaaac aggttgcggc tattcttcac gatccagaag
     4981 ccagcgaaaa cgacaagtac gtggcactgc aattcttaag aaactccgaa atcgccgcca
     5041 aaggcgtgct gccgacctgc caggataccg gcaccgcgat catcgtcggt aaaaaaggcc
     5101 agcgcgtgtg gaccggtggc ggtgatgaag aagcgctgtc gaaaggcgtc tataacacct
     5161 atatcgaaga taacctgcgc tactcgcaaa acgcggcgct ggacatgtac aaagaggtca
     5221 acaccggcac taacctgcct gcgcaaatcg acctgtacgc ggtagatggc gatgagtaca
     5281 aattcctttg cgttgcgaaa ggcggcggct ctgccaacaa aacgtatctc taccaggaaa
     5341 ccaaagccct gctgactccc ggcaaactga aaaacttcct cgtcgagaaa atgcgtaccc
     5401 tcggtactgc agcctgcccg ccgtaccata tcgcgtttgt gattggcggt acgtctgcgg
     5461 aaaccaacct gaaaaccgtc aaattagcaa gcgctcacta ttacgatgaa ctgccgacgg
     5521 aagggaacga acatggtcag gcgttccgcg atgtccagct ggaacaggaa ctgctggaag
     5581 aggcccagaa actcggtctt ggcgcgcagt ttggcggtaa atacttcgcg cacgacattc
     5641 gcgttatccg tctgccacgt cacggcgcat cctgcccggt aggcatgggc gtctcctgct
     5701 ccgctgaccg taacattaaa gcgaaaatca accgcgaagg tatctggatc gaaaaactgg
     5761 aacacaatct aggtcagtac attccacaag aactgcgtca ggccggtgaa ggcgaagcgg
     5821 tgaaggtcga ccttaaccgc ccgatgaaag agatcctcgc ccagctttcg caatatccgg
     5881 tttccactcg tttgtcgctc actggcacca ttattgtggg tcgagatatt gcacacgcca
     5941 agctgaaaga gctgattgac gccggtaaag aacttccgca gtacatcaaa gatcacccga
     6001 tctactacgc gggtccggcg aaaacccctg ccggttatcc atcaggttca cttggcccaa
     6061 ccaccgcagg tcgtatggac tcctatgtgg atctgctgca atcccacggc ggcagcatga
     6121 tcatgctggc gaaaggcaac cgcagccagc aggttaccga cgcgtgtcat aaacacggcg
     6181 gcttctacct cggtagcatc ggcggtccgg cggcggtact ggcgcagcag agcatcaagc
     6241 atctggaatg cgtcgcttat ccggagctgg gtatggaagc tatctggaaa atcgaagtag
     6301 aagatttccc ggcgtttatc ctggtcgatg acaaaggtaa cgacttcttc cagcaaatcg
     6361 tcaacaaaca gtgcgcgaac tgcactaagt aacctcttcg gcccagcgcc tgacagcatg
     6421 ctgccaggtg atccccctgg gccacctctt ttgcgattgt aatttcatgc ttgctggtga
     6481 atagtcagta ttttccctga cttgcgaact caccatgacc cgcacactca agccgttaat
     6541 tcttaacacc ggcgcactgg cgctaacgtt aatcctgatt tataccggca tttcggccca
     6601 tgacaaactc acctggctgc tggaagtgac accggtgatt atcgtcgtgc cgctactact
     6661 tgccaccgcc agacgttacc cgttaacgct gctgctctat acgctcatct tctttcacgc
     6721 catcatcctg atggtaggcg ggcagtacac ctacgcgaaa gtccccgttg gttttgaggt
     6781 gcaggaatgg ctggggttga gtcgtaatcc gtatgacaag ctggggcact ttttccaggg
     6841 gctggtgcct gcactggtgg cacgagaaat tctcgttcgc gggatgtacg tgcgcggacg
     6901 taaaatggtg gcgtttctgg tctgttgcgt ggcgctggcg ataagcgcga tgtatgaatt
     6961 aatcgagtgg tgggcggcac tggcgatggg ccagggagcg gatgattttc tcggtaccca
     7021 gggcgaccag tgggacacgc aatccgatat gttctgcgcg ctgcttggcg cattaacgac
     7081 agtgatattg ctcgctcgct ttcattgccg ccagctacgg cgctatggct tgataactgg
     7141 ataacaacgt gcactttgtt gatgccggat gcggcgtgaa ttccttatcc ggtctacaaa
     7201 atttgcaaat ccaaagcatt acattaagac gtggcctgat aaacatatcg catcaggcca
     7261 ttttcgttag ctattgtcca cgtcaggctt tcacatcact cactgcaccg accggaatat
     7321 cagcatgaac aggctcgggc ggtactttgc gatatttatc cagcagatga atctggatcc
     7381 tgcgcagcgt gtcgccattg aggcgataga aacggaagta gagaatcagc gttaccatga
     7441 aaaacagagt cggtagagca atcataataa actgcatacc taatagggct tgcgtagact
     7501 gttcaacatt cggtacatag ccaatcatcc ctaacacaac cgcaatgaaa aaagccgcaa
     7561 aggctgaacc gcccttcacc accatagtct gcacggagta agcgatgctt tcacagcgta
     7621 cgtgcagttt atattcaccg taatcaacgg tatctgccac catgatgacc tgtaataccc
     7681 agaaaagcgc cgttcccaca ttcagcaaaa tacccgcaat cacaatgagg acgacgttgt
     7741 gatagctcat taatgccatt aacaggagaa caccacagct taacaccgga agaatagatg
     7801 ctccggccca taaaatgcgt cgggataatg atttaactaa gcgtgggaag aatactaacg
     7861 tcaccaggtt agcagctccc gcatacgaca gataataggg gaacaaatcc gcatcaccga
     7921 taacatatga gaaataatag atagcaaatc cggtaataat gttgctggct acattataag
     7981 caagagccat acccaagagg catgaaagct gatcgttttt ataaataagc gcaacgatgg
     8041 ctttaagtgt cagatggctt ccttcagcag acgattgatt gtctgacgaa aagacttcat
     8101 gcacattgcg cagagtgatg atggttgaaa caataaaaaa ggcgatcaat accagagtga
     8161 acatctgaaa gccaaatccc cgatcgccac cgccgacata attaacaaat ggtagcgtca
     8221 cacctgccgt aacaaagcct gccagactgg caaaaaaacg cggataagga accagttgtt
     8281 cgcgttcacg tttatcgagc gtgatggttg gaaccagcga ccagaaggga atatccataa
     8341 tggtgtaagt catgccccag aggatgtagg tcacgcaaac aaagacaatc tgagtagtac
     8401 cttcaaacag atgcgcacta aagaggagaa ataagattac agagtttgcc aacgtaccga
     8461 tcaggatcca gggtttgaac ttaccccatc gcgatcgcgt agcatttaca atccatccca
     8521 taatcggatc gttaatagca tcccagatcc tcgccaccag aaacaaagta ccgaccaaac
     8581 ccacagacag cccgacgaca tcggtgtagt aatacatgag gtacatatac acaatgccga
     8641 tcgcaaaatc cttcccgaac gctccaaatc cataactgag ttttgtagtc attgaaatgc
     8701 tcatagggta tcgggtcgct gttaccaacg cccgccttct gttattggta tcgtcagagg
     8761 tgtgcgccgg aataaatccc ccggcgcagt agcgtttagt cgcgttttaa cggtgcaact
     8821 agcttggcag ccagtcgccg tgggcggcaa tcaggtcgtc aacaagagca tatatttcgt
     8881 caatgcccag cacggcggca gtatgcgggt ccatcatcgc ggcgtggtaa acacggtcgc
     8941 gattttccgt aagaatagct tcggtcagca gcgtctgtac gttgatgttg gtttgcatca
     9001 gggcggccag atgcgaaggt agcgtaccga ctttggtcgg ctgaatgcca ttagcatcaa
     9061 ccagacaggc tacttccacg caacatcctt gtggcaggtt atcaatcaaa ccatcgttac
     9121 ggacgttgcc gtaaatcaca ctcggctcgc cagtccagat agcgttcatg attgtgctgg
     9181 catattcccg tgacggttta atatcaatcc gggaggcgtt tttatactcc tccagctctt
     9241 tatgccagtt cgccagctgc tcgacgcagc gtttcgggta ctcatccagc ggtactttat
     9301 aacgctcaat caaatcctca cgacctagct taataaacca cggcgtatat tcggcaaaat
     9361 gttctgacga ttccgtgacg aagtagccca gctttttgaa catttcgtag cgcacaatat
     9421 tctggcagcg agtattacca tgaatatttg gcttcggtgc ctgccctgct tcataagccg
     9481 ccagcagttc cgggtagaga ttcacgtaac tgccgtcggc ggttttgcgc tccagctcca
     9541 ggtaaaacgc catatggttg atacctgcgc aacggtaacg cagcgtagcc gggtcgatat
     9601 tgaggtcacg cgccagctct tccgccgttc cctgcaccga atggcacagc ccgacctgtt
     9661 tgatatacgg atagcgggca tacatcgccc aggtattcat cgccattggg ttaacatagt
     9721 tgagcatggt ggcatcgggg cagacttccg tcatgttctc gcaaatttgc cacagatgcg
     9781 gaatggtacg tagcgcgcgc ataataccgc ccggccccaa cgtatcggca atggtttgtt
     9841 ccagaccatg ccgcttacag acctcgaaat cagtcaccgt gcaaggttca taaccgccaa
     9901 tctgaaatgc caccacgaca aaatcggcat cctctaaggc ttctttctgt tgggtgtggc
     9961 aggtgatttt gccgctggcc cctgctgaat ccatcagctt acgcaccaca atatgcgact
    10021 cttccaggcg ggtggggtca atgtccatca gggcaatatg cgccgttttc agcgcctcgc
    10081 gatggaacac atcaccaaga atatttttaa cgaaaatcgt cgaaccagcg ccgataaatg
    10141 taattttggg tgcagacatc atgcagatct cctggcttgc ttgaataact tcatcatggc
    10201 aggcgaatct gcagaaaacc tccggcttcc aggttgagca ttcccataaa ctcagatcct
    10261 gcgtgaagca gcagtaaatc tgagtttatg ggaataaatt acctgaaaag tagagttttc
    10321 ggcctcatga tgctcggaca gtcccctcgc ccctctggga agagggtgaa aaccgtgctc
    10381 ccactcgcag tcatcctccc tcactcctgc cataattctg atattctagg aaagagagcc
    10441 atccatgaat acagatacgt ttatgtgcag cagcgacgaa aaacagaccc gcagcccgct
    10501 gtcgctgtat tcggaatacc agcggatgga gattgagttt cgcgcaccgc atatcatgcc
    10561 caccagccac tggcatggtc aggtcgaagt gaatgtgcct ttcgatggcg atgtggaata
    10621 cctgatcaac aatgaaaaag tgaatatcaa tcagggtcat atcacgctgt tctgggcctg
    10681 tacaccgcac caactaacag ataccggaac ctgtcagagc atggcgattt ttaatctgcc
    10741 gatgcatctg tttctctcct ggccgctgga taaagacctg attaaccacg tcactcacgg
    10801 catggtgatc aaatcacttg ccacacagca acttagcccg tttgaagtac gccgctggca
    10861 gcaggaatta aacagtccga atgagcaaat tcgccagctc gccattgatg aaattggcct
    10921 gatgctcaag cgatttagcc tctctagctg ggaaccgatt ctggtcaata aaacctcgcg
    10981 cacacacaaa aacagcgtct cgcgccatgc gcaattttat gttagccaga tgctgggctt
    11041 tattgccgaa aactatgatc aggcgctgac catcaacgat gtggctgagc acgtcaaact
    11101 taacgccaac tatgcaatgg ggatattcca gcgggtcatg caattgacga tgaaacagta
    11161 cattaccgcg atgcgcatca accacgttcg cgcgttactg agcgataccg ataaaagtat
    11221 tctcgatatt gccctgacgg caggctttcg ttcaagtagt cgtttttaca gcacgttcgg
    11281 caaatatgtc ggcatgtcgc cgcaacaata ccgcaaactt agccaacagc gccgccagac
    11341 gtttcccggc taaagacgat atcagtatca gccaaaaaaa tagtttagcc gtcgataata
    11401 agcagtgaaa ataatttaaa ataatcacat aacgtattgt ttataaaact aaatatttca
    11461 aaaatgtttg tttttcacgc gctttacagc ccgaaaaggc cggaagatac ttgcccgcaa
    11521 cgaagattcc ttcataaccg ggtaagcaat gatgaaagta ttaattgttg aaagcgagtt
    11581 tctccatcaa gacacctggg tcggtaacgc cgttgagcgt ctggcagatg ctttaagcca
    11641 gcaaaatgtt accgtgatta aatccacctc ctttgatgat ggttttgcca ttctctcttc
    11701 aaacgaagcc attgactgcc tgatgttcag ctatcaaatg gagcatccgg acgaacatca
    11761 aaacgtcaga caattgatcg gtaagcttca tgagcgccaa caaaacgtgc cggtcttcct
    11821 gttgggcgat cgggaaaaag ccctcgccgc aatggaccgg gatctgctgg agcttgtcga
    11881 tgaattcgcc tggattctgg aagataccgc cgactttatc gccggacgcg ccgttgccgc
    11941 gatgacccgc taccgccagc agctgttgcc gccactgttc agcgcgctga tgaaatatag
    12001 tgacatccat gaatattcct gggcagcgcc aggccaccag ggcggcgttg gttttaccaa
    12061 aacacccgcc ggacgtttct accatgacta ctatggtgaa aatctgttcc gcaccgacat
    12121 gggcatcgaa cgaacttccc tcggttcttt gcttgaccat actggcgcat ttggcgaaag
    12181 cgaaaaatat gccgcacgcg tatttggtgc cgatcgctcc tggtcggtag tcgtcggtac
    12241 ttccggctct aaccgcacca tcatgcaggc ttgcatgacc gataacgatg tcgtggtcgt
    12301 tgaccgtaac tgccataaat ccatcgaaca aggtttgatg ctgacaggcg cgaaaccggt
    12361 ctatatggtg ccaagccgca accgctacgg cattatcggg ccaatctatc cgcaggaaat
    12421 gcaacctgaa accttgcaaa agaaaatcag tgaaagcccg ctgaccaaag acaaagccgg
    12481 gcaaaaaccg tcttactgcg tggtaaccaa ctgcacctat gacggcgtgt gttataacgc
    12541 taaagaagcg caggatctgc tggaaaaaac ctccgatcgt ctgcactttg acgaagcctg
    12601 gtacggctat gcacgtttca acccgatcta tgccgatcac tatgccatgc gcggcgaacc
    12661 tggcgatcac aacggtccta ccgttttcgc cacccactcc atccacaaac tgctgaatgc
    12721 gctgtcacag gcttcttata ttcatgtacg tgaaggtcgt ggggcgatta acttctcccg
    12781 cttcaaccag gcctacatga tgcatgccac cacctccccg ctgtatgcca tctgcgcatc
    12841 caacgacgtg gcggtgtcga tgatggacgg caacagcggc ctgtcactga cacaggaagt
    12901 gattgacgaa gcggttgatt tccgtcaggc gatggcgcgg ctatataaag agttcaccgc
    12961 tgacggtagc tggttcttca aaccgtggaa caaagaagtc gtcaccgacc cacaaaccgg
    13021 caaaacctat gactttgctg acgcaccaac caaactgctg accaccgttc aggactgctg
    13081 ggtaatgcat ccgggcgaaa gctggcacgg cttcaaagat attccggata actggagtat
    13141 gctcgaccca attaaagtca gcatccttgc tccgggaatg ggtgaagatg gtgaactgga
    13201 agaaaccggt gttccggcgg cgctggtcac tgcctggctt ggtcgccacg gcattgtgcc
    13261 tacccgcacc actgacttcc aaattatgtt cctgttctct atgggcgtaa cccgtgggaa
    13321 atgggggact ctggttaaca ccctttgctc cttcaaacac cactatgacg ccaacacacc
    13381 gctggcgcag gtgatgccgg aacttgttga acaatatcct gacacttacg cgaacatggg
    13441 gattcacgat ctgggtgaca ccatgtttgc ctggctgaaa gaaaacaacc ctggcgcacg
    13501 gttgaacgaa gcctattccg gcctgccggt ggcggaaatc accccgcgtg aagcgtacaa
    13561 cgcgattgtc gacaacaatg tcgaactggt atccattgaa aatctgccag gacgcatcgc
    13621 ggcaaactca gttatcccgt atccgccagg aatcccgatg ctgctgtctg gtgaaaactt
    13681 cggcgataaa aacagtccgc aagtaagtta tttacgctcg ctgcaatcct gggaccacca
    13741 tttccctgga tttgaacacg aaactgaagg gactgaaatt attgacggta tttaccacgt
    13801 tatgtgcgtg aaagcgtaac cactattccg ctgaaggcgt aattgtttaa ataacattac
    13861 gccgcctggc cttaggcctt ttgagtatgg caacgttttc ataaaaattg ctgcaaacaa
    13921 aaatgtcata ctttttgcgc ggccccaccc cgcgcttttt tttgcctgtt atttatcctg
    13981 taaaaaaata tgtacatgag aaattactat aaaaatttgt aatattagta aaactcgtta
    14041 tttttatgta tgtttatatt catcatgcaa ttatataacc atttcccggt atcgctttgc
    14101 tttagcgaga actggtcttt tattcgcact caggagtaca tgtatgagga tttgcagcga
    14161 ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga atgggaaaga
    14221 acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata atattatcga
    14281 cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca tcaaagatta
    14341 cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta gcgctacgcc
    14401 catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc aacacagcat
    14461 gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat tcacggtgct
    14521 atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt tacgtaattg
    14581 tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact ggaatcttag
    14641 tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagt tgaaaagcga
    14701 aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg taaatgaatt
    14761 aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca acagtacgtc
    14821 gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt atgttagtca
    14881 gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata ttaataacgg
    14941 catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta tgatatgacg
    15001 taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg gatgctgatg
    15061 ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt atggggtcag
    15121 gtgtttttct gttacctgca aacctggcct ccactggcgg gattgccatt tatggatggt
    15181 tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg tcgttcctcg
    15241 acccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg tttctcggtt
    15301 atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc atggtggtca
    15361 ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg gtattaacca
    15421 tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt ccgaaaatga
    15481 tcacccgcgt gcaggccgtt gccactgtgc tggcgctgat ccccatcgtc gggattgccg
    15541 tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac gtcagcggcc
    15601 tgggcacctt cggtgcgatt caaagtaccc ttaacgttac gctgtggtcg ttcatcggtg
    15661 tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat gtccctatcg
    15721 ccaccattgg tggggtattg attgccgccg tttgctatgt gctctccacc accgcgatta
    15781 tggggatgat ccctaatgcc gcactgcgcg tttctgcctc gccattcggt gatgccgcac
    15841 ggatggcgtt gggtgacacc gctggggcca ttgtttcctt ctgcgcagct gcgggttgct
    15901 taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc gctgccgatg
    15961 acggactgtt cccaccgatt tttgcccgtg taaataaagc gggtacgcca gtggcggggc
    16021 tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca ccaaacgcga
    16081 ccaaagagtt cggtctggtt tcttccgtct cggtcatctt tacactggtg ccatatcttt
    16141 acacctgtgc ggcgttactg ctgctcggcc acggtcactt tggtaaagca cgcccggcat
    16201 atctggctgt tactaccatt gccttcctct actgcatttg ggccgtggtg gggtccgggg
    16261 cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat caccgccatg tatgcgctga
    16321 attacaaccg gctacataaa aacccgtatc ccttagatgc accaataagc aaagattaat
    16381 tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg cttaattttg
    16441 ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa gcgcctacta
    16501 aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta tatctctatc
    16561 tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga ttcgctgcat
    16621 aacgtactgg ttttcttgtc gatgccggtc gtcgctttca gcgtgattaa tattgtcctg
    16681 acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat tctgattggc
    16741 gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc gatgattgcc
    16801 aatattattg ataccactcc agcagaaagt tatgcgctga tgacaccgca aatgttatta
    16861 acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggctaaa aatcaaacct
    16921 gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt ttctgtactg
    16981 ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt ccgcaataac
    17041 aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg gtcatggtac
    17101 tcccatcagc gactggcaaa tctgccgctg gtgcgaattg gtgaagacgc gcaccgcaac
    17161 ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg cgaaacctcg
    17221 cgggcggaga acttctccct caacggctac ccgcgtgcaa ctaacccgcg gctggcgaaa
    17281 gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc agtttcagta
    17341 ccgtgcatgt tctcggatat gccgcgtgag cactacaaag aagagctggc acagcaccag
    17401 gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa tgacaacgat
    17461 ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc gctgaatctg
    17521 cctgatcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg gctggaagag
    17581 tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg cagccacggt
    17641 ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac ctgcgacacc
    17701 aatgagatcc agacctgtac caaagagcaa ctggtgaaca cttacgacaa cacgctggtt
    17761 tacgtcgact atattgttga taaagcgatt aatctgctta aagaacatca ggataaattt
    17821 accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa tggcatctat
    17881 ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc aatgctgctg
    17941 tggctgtcgg aggattatca aaagcggtat caggttgacc agaactgcct gcaaaaacag
    18001 gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg attaactggc
    18061 gttgagacga agtattacca ggccgcggat gatattctgc aaacttgcag gagagtgagt
    18121 aaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggactg attctggcgg
    18181 cgcaaaccga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg gaacaaagcc
    18241 ttgaggacgg tcattacagc ctggtggtac tggatttagg gttacccgac gaagatggac
    18301 tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccggtactg atcctcaccg
    18361 ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt cggtgccgat gactatctgg
    18421 tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta cgacgccata
    18481 ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg ggtcgccgtc
    18541 aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct ctgctgtcac
    18601 ggttaatgct caaagcaggc agtccggtac atcgggaaat tctctacaac gacatctata
    18661 actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat ctgcgcgaca
    18721 aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg gtcgcgaatg
    18781 aggaaaacta attgaatctg atgcgttttc tgcgccgacc aatatcgctg cgccaacggc
    18841 tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc ttctggctat
    18901 ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac aatcgcaaca
    18961 acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt gtccccggcg
    19021 tctttatggt cagcctgacg ctatttatct gctatcaggc ggtacgccgc atcacccgcc
    19081 cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgacaactta acgcccattg
    19141 ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttgaac gatctggtca
    19201 gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc gcgcacgaac
    19261 tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa acgcatcaca
    19321 ttgatgtagc accgttagtg gcacggcttg atcagatgat ggagagcgtc tcccagctgc
    19381 tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat gtaaaactgc
    19441 tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac cagcgacagc
    19501 aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat gcaaccctgc
    19561 tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg caaggcagca
    19621 acattatgat taagctgcaa gaagatggcg gagcggtcat ggcagttgaa gatgaaggac
    19681 caggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt atggacagcc
    19741 gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag ttgcatcacg
    19801 ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg gtacggctga
    19861 agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc agcacgctga
    19921 acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc gttaacctgg
    19981 aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc ctgataagac
    20041 agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt tgctgcacca
    20101 ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg attttctgct
    20161 cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct gacgccgccg
    20221 gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg gtgattaaac
    20281 caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc gagctttcga
    20341 ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata ttaaatgccg
    20401 ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta gaagccataa
    20461 cgcccataaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa atcaggccga
    20521 tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagt gcaacactac
    20581 caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc accggctgga
    20641 caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct tcggagtaat
    20701 gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg
    20761 tggcaattac cagaccaata catgtcaaca ggctgcgcca gtatttagtg gcaatctctt
    20821 taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc agtttatcaa
    20881 catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc ccgataatcc
    20941 ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag ttcgcttcgc
    21001 cgacaatggt cgaaattaac accaccacgc ccgcacccag cacaaacccg gcaatagaac
    21061 cgaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat tccgcaacaa
    21121 atatcgacgc cccggtatat tcaccaccga ccgagaaacc ttgtgccatc ttacagatca
    21181 acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag ccaatacaga
    21241 acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga ccatatttat
    21301 cgcccaacat accaaagaag agtccgccaa gcggtcgaat cagaaaggga acggagaaag
    21361 tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta
    21421 atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca ttacccagtg
    21481 atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca tcacgaaggg
    21541 taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg gcccatttcc
    21601 gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca
    21661 atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat gtacaatcaa
    21721 accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt gtttaaattt
    21781 gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga ttatttacgc
    21841 caaatttaca gagaagattt cctcgaccat tgcccccacc aaacgctgcg ctgaacagag
    21901 tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc aaacgggttg
    21961 tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt
    22021 cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtagtcg gtgccgcatt
    22081 gatactctct gccagatgtt gtgtcgcttt ctcgcgcaaa tcacccagca acaacaccac
    22141 cgcttgcggg cttcccagac ggcaggcatc gccaaatgca ccccagcgca actcctccag
    22201 aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt
    22261 aaaaatttgc tcgctctgat cacctgcctg caccagtagg cctcgttgcg ttaacgcctt
    22321 ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggcaattt gctgggggag
    22381 ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgctgcgc
    22441 gatctgcgtc agcgccagat gaccgggatc cagcatcgac gccagttttt ccaccattgt
    22501 cagtcgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct gaagctgcac
    22561 acgcgaaaga agttcctgca ttagtcgttc acgaaactgc tgctgatggg cttgaggatg
    22621 gcgggcatca tcgccctgcg ccagatccac taaaaagcgc ggatgaaccg actccagcac
    22681 gcgaccgggg ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga
    22741 atatcatcgc gtaacagctg agcgtcggtg taaatccagc tgtgagtcat cacagtctgc
    22801 tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag agccagactt
    22861 tcgcgtaaac gcgtctgtaa ttctgtcaaa catgaagcga actcagcgaa aaaagtattc
    22921 atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat ttgttggcaa
    22981 tagagatcga cttctgcgct taatgctcgt tgcaacacac tgtaatcaac cgtttctgtc
    23041 ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga aaaagtctca
    23101 cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt cctggcgcga aatgacttca
    23161 ctgaacagct ggcgagtgtt gaagtgggga actacgccgt gaaaaacagg aaaatgaaac
    23221 ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat ggggcgaata
    23281 acatcggtta acacccggca aagggtggat tccatctcgg caaaacgcag cgtaaagtcg
    23341 cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg aatctcgctt
    23401 aacggctccg aatcatcctg aaacaaggcg gctaactgcg cattcaccgc atcttgctgt
    23461 tgacgcagaa agtggttggc ggaggtcagg gccagctcga tttcatgttt aatctcgccg
    23521 ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt
    23581 tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc
    23641 gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc gcagagcata caatgaggcg
    23701 ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac
    23761 aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc
    23821 ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cgctggtaac
    23881 ttaccgttgt tggctaactc atagcgcgcc cgattcgcca ggtagcccca catcgacgac
    23941 accgggaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga aatcagtgcc
    24001 cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca
    24061 tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc atcggagatc
    24121 gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg
    24181 ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac
    24241 gtcaactgac cgggataact ttccagcccc gccagatgga caaactccac ttcaatcacg
    24301 ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc
    24361 agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc acccagataa
    24421 tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat atctttatct
    24481 atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt
    24541 tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc cttttgcccg
    24601 ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt aggcagaacc
    24661 tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt accgacaatt
    24721 gccagcacca tttccagtcg ggaaatttta cacaactcat tattcagcat cgcgtgacgt
    24781 tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcgtcgtc tccgggtaca
    24841 gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca
    24901 gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc cttaaagcaa
    24961 attttgttac ttttatttag ccagattgtt tttgagttct gttttcggct tttataatta
    25021 ctgcaagaaa taattttata tttagtgtgt tgttttttat cagaataaat aacgtcttct
    25081 gatgcgttta aaacgtcaga aagataaaaa tatcatgtga ataaaaaaaa gaacaagtag
    25141 agcattaaca ttatcttaaa taataaatag aggcaaaaag attattttct ttttacgttt
    25201 cctttcaaat gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa actcagggat
    25261 tttgtgtata attgcggcct ttttcggcaa tctgccgttt tttggcgctt ttgccctgct
    25321 gacttttgag gaaatccaca tgtcattacc acactgccca aaatgcaact ccgaatacac
    25381 ttacgaagat aacggcatgt acatctgccc ggaatgtgcc tacgaatgga acgacgcaga
    25441 acctgcacag gaaagcgacg agctgatcgt taaagatgct aacggcaatc tgctggctga
    25501 cggcgacagc gttaccatca ttaaagatct gaaggtgaaa ggtagctctt cgatgctgaa
    25561 aattggcacc aaagtgaaaa acatccgcct ggttgaaggc gaccataaca tcgattgcaa
    25621 aatcgacggt tttggtccga tgaaactgaa atctgagttt gtgaaaaaga actgattgta
    25681 ttgtgatcgg taagccggat aaggcgctcg cgccgcatcc ggcaacgatg ccgactgcct
    25741 gatgcgacgc ttgcgcgtct tatcaggcag cctctcatct gtataaattt cgaactacac
    25801 ttaactggct tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg
    25861 ccggtaactg ttccgacgcg attgcctatt atcaacgtac gttgggcgcg gaactgctct
    25921 ataaaatcag cttcggcgaa atgccgaaat cagcgcagga cagcgccgag aactgccctt
    25981 ccggaatgca atttcccgat accgccatcg ctcatgccaa cgtgcgcatt gccggaagcg
    26041 acatcatgat gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg
    26101 tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg
    26161 gaaaaatcga aatggcctgg caggaaactt tctgggcgca tggctttggc aaagtcaccg
    26221 ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg
    26281 ccgggaggcc cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc
    26341 acttttaatt aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag
    26401 gctgcatcat gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc
    26461 aagcgctgca tgcggttgat ctgaacattc atcacagtga aatggtggct ctgcttgggc
    26521 cgtcgggttc cggcaaatcc acccttttac gtcatttaag cggtttgatt accggcgata
    26581 aatccgccgg tagccatatc gagctactgg gccgcactgt ccagcgcgaa ggccgcctgg
    26641 cccgcgatat ccgcaaaagc cgcgccaaca ccggctacat ctttcaacaa ttcaacctgg
    26701 tgaaccgcct gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct
    26761 ggcgcacctg ttttagctgg ttcaccggcg agcagaaaca gcgcgcgtta caggcgctga
    26821 cccgcgttgg catggtgcat tttgcccatc agcgcgtttc caccctctcc ggcggccagc
    26881 agcaacgtgt ggcgattgcc cgtgcgctga tgcagcaggc gaaagtgatt ctggccgatg
    26941 aacccatcgc ctcgctggac ccggaatcag cgcgcatcgt gatggacacc ctgcgcgaca
    27001 tcaaccagaa cgacggcatc accgtggtcg tcacgctgca tcaggtggat tacgccctgc
    27061 gctactgcga acgcatcgtc gccctgcgtc aggggcacgt cttctacgac ggcagcagcc
    27121 aactgtttga taacgaacgt tttgaccatc tctaccgcag cattaaccgc atcgaagaga
    27181 acgcgaaagc tgcctgatat ccccatcatt gaggaaaacg aatgaacgct aagataattg
    27241 cctcgctggc cttcaccagc atgttcagcc tcagcaccct gttaaccccg gcacacgccg
    27301 aagagcagga aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga
    27361 aaccgcaatg gacgccgttt ttacaggata tggagaagaa actgggcgtg aaggtcaacg
    27421 ccttctttgc cccggactac gcgggcatta tccaggggat gcgcttcaat aaagtggata
    27481 ttgcctggta cggcaacctg tcggcgatgg aagcggtgga tcgcgccaat ggccaggtct
    27541 tcgcccagac ggtcgcggcg gatggatcgc cgggttactg gagcgtgttg atcgtcaaca
    27601 aagacagccc gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg
    27661 gcaatggcga tcctaactcc acctctggct tcctcgtccc cggttactac gtcttcgcta
    27721 aaaacaatat ctccgccagc gacttcaagc gcaccgtcaa cgccgggcat gaaaccaacg
    27781 cgctggccgt cgccaacaag caagtggatg ttgccaccaa caacaccgaa aacctcgaca
    27841 agctgaaaac ctccgcgccg gagaagctta aagagctgaa ggtgatctgg aaatcgccgc
    27901 tgatcctagg cgatccgatc gtctggcgca agaatctgtc cgaaactacc aaagacaaga
    27961 tctacgactt ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc
    28021 tgggctgggc gccgttccgc gcttccagcg acctgcaact ggtgccgatt cgccagctcg
    28081 cactgtttaa agagatacaa agcgtgaaag gcaataaagg gctgaatgag caggacaagc
    28141 tggcgaaaac caccgagatt caggcgcagc tggatgacct ggaccgcctg aacaacgcgt
    28201 taagcgcgat gagttcggtg agtaaagcgg tgcagtaaat cgtaggtcgg ataagacgcc
    28261 ccggcgtcgc atccgacaat gtgtaggcgt tgatgccgga tgcggtgcaa gcaccttatc
    28321 cggcctacag accggagcca aacatgcaaa ccatcaccat cgccccaccc aagcgtagct
    28381 ggttctcgct tctgagctgg gccgttgttc tcgacgtgct ggtcgtctcg tggcagggcg
    28441 cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc tt
//
LOCUS       DAJTNW010000054        28421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNW010000054 DAJTNW010000000
VERSION     DAJTNW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            564..1334
                     /locus_tag="OO179_002373"
     CDS             564..1334
                     /locus_tag="OO179_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5532407.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO179_002374"
     CDS             1325..1798
                     /locus_tag="OO179_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5532408.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO179_002375"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO179_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5532409.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO179_002376"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO179_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5532410.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO179_002377"
     CDS             3233..3727
                     /locus_tag="OO179_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5532411.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO179_002378"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO179_002378"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5532412.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO179_002379"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO179_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="OO179_002380"
     CDS             7571..8485
                     /locus_tag="OO179_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5532413.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="OO179_002381"
     CDS             8700..10061
                     /locus_tag="OO179_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532414.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO179_002382"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO179_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5532415.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO179_002383"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO179_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5532416.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="OO179_002384"
     CDS             12611..13900
                     /locus_tag="OO179_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5532417.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="OO179_002385"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="OO179_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5532418.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="OO179_002386"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="OO179_002386"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5532419.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="OO179_002387"
     CDS             16226..16606
                     /locus_tag="OO179_002387"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5532420.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="OO179_002388"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="OO179_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5532421.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="OO179_002389"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="OO179_002389"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5532422.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="OO179_002390"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="OO179_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5532423.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="OO179_002391"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="OO179_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5532424.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="OO179_002392"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="OO179_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="OO179_002393"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="OO179_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="OO179_002394"
     CDS             23335..23847
                     /locus_tag="OO179_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5532425.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="OO179_002395"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="OO179_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5532426.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="OO179_002396"
     CDS             26440..26643
                     /locus_tag="OO179_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5532427.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="OO179_002397"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="OO179_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5532428.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28421)
                     /locus_tag="OO179_002398"
     CDS             complement(27679..>28421)
                     /locus_tag="OO179_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5532429.1"
                     /translation="ENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNLD
                     DFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSG
                     FIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEY
                     WINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDV
                     CNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7431 c         7126 g         6909 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct a
//
LOCUS       DAJTNW010000055        28363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNW010000055 DAJTNW010000000
VERSION     DAJTNW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            229..336
                     /locus_tag="OO179_002399"
     CDS             229..336
                     /locus_tag="OO179_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5532430.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="OO179_002400"
     CDS             712..819
                     /locus_tag="OO179_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5532431.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="OO179_002401"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="OO179_002401"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5532432.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="OO179_002402"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="OO179_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5532433.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="OO179_002403"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="OO179_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5532434.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /locus_tag="OO179_002404"
     CDS             4614..4802
                     /locus_tag="OO179_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5532435.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="OO179_002405"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="OO179_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5532436.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..5922
                     /locus_tag="OO179_002406"
     CDS             5563..5922
                     /locus_tag="OO179_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5532437.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5919..8180
                     /gene="bcsA"
                     /locus_tag="OO179_002407"
                     /pseudo
     CDS             5919..8180
                     /gene="bcsA"
                     /locus_tag="OO179_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10530
                     /gene="bcsB"
                     /locus_tag="OO179_002408"
     CDS             8191..10530
                     /gene="bcsB"
                     /locus_tag="OO179_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5532438.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            10537..11643
                     /gene="bcsZ"
                     /locus_tag="OO179_002409"
     CDS             10537..11643
                     /gene="bcsZ"
                     /locus_tag="OO179_002409"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5532439.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11702..11878
                     /locus_tag="OO179_002410"
                     /pseudo
     CDS             11702..11878
                     /locus_tag="OO179_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            12206..13954
                     /gene="bcsC"
                     /locus_tag="OO179_002411"
                     /pseudo
     CDS             12206..13954
                     /gene="bcsC"
                     /locus_tag="OO179_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            14036..16024
                     /gene="hmsP"
                     /locus_tag="OO179_002412"
     CDS             14036..16024
                     /gene="hmsP"
                     /locus_tag="OO179_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5532440.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16209..17495
                     /gene="dctA"
                     /locus_tag="OO179_002413"
     CDS             16209..17495
                     /gene="dctA"
                     /locus_tag="OO179_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5532441.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17716..19212
                     /locus_tag="OO179_002414"
     CDS             17716..19212
                     /locus_tag="OO179_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5532442.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(19308..20237)
                     /gene="kdgK"
                     /locus_tag="OO179_002415"
     CDS             complement(19308..20237)
                     /gene="kdgK"
                     /locus_tag="OO179_002415"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5532443.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20469..21236
                     /gene="pdeH"
                     /locus_tag="OO179_002416"
     CDS             20469..21236
                     /gene="pdeH"
                     /locus_tag="OO179_002416"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5532444.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            21306..23366
                     /locus_tag="OO179_002417"
     CDS             21306..23366
                     /locus_tag="OO179_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5532445.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23548..24870)
                     /locus_tag="OO179_002418"
     CDS             complement(23548..24870)
                     /locus_tag="OO179_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5532446.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25281..26294)
                     /gene="yhjD"
                     /locus_tag="OO179_002419"
     CDS             complement(25281..26294)
                     /gene="yhjD"
                     /locus_tag="OO179_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5532447.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26343..27242)
                     /locus_tag="OO179_002420"
     CDS             complement(26343..27242)
                     /locus_tag="OO179_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532448.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            27762..>28363
                     /locus_tag="OO179_002421"
     CDS             27762..>28363
                     /locus_tag="OO179_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5532449.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6770 a         7145 c         7792 g         6656 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccaaggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca ataatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgtgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa ctctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 agtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
    10561 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
    10621 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
    10681 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
    10741 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
    10801 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
    10861 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
    10921 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
    10981 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
    11041 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
    11101 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
    11161 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
    11221 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
    11281 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
    11341 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
    11401 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
    11461 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
    11521 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
    11581 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
    11641 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
    11701 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
    11761 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    11821 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    11881 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
    11941 actggcgctg ggccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
    12001 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
    12061 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
    12121 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
    12181 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
    12241 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
    12301 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
    12361 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
    12421 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
    12481 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
    12541 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
    12601 gatggaaagc gcgatggtac tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
    12661 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
    12721 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
    12781 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
    12841 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
    12901 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
    12961 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
    13021 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
    13081 cagcgtggcg gtcggctggc gaaatgacgt ctggagctgg gatatcggta ccacgccgat
    13141 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
    13201 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
    13261 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
    13321 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
    13381 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
    13441 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
    13501 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
    13561 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
    13621 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
    13681 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
    13741 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
    13801 ttccaactgg tttgttggta cggcaattga tatccagcag gcgaaagatt acgcacccag
    13861 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    13921 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    13981 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    14041 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    14101 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    14161 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    14221 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    14281 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    14341 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    14401 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    14461 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    14521 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    14581 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    14641 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    14701 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    14761 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    14821 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    14881 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    14941 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    15001 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    15061 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    15121 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    15181 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    15241 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    15301 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    15361 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    15421 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    15481 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    15541 cttctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    15601 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    15661 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    15721 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    15781 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    15841 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    15901 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    15961 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    16021 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    16081 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    16141 tgttatttta aggccgcagg taccccccat aaccttacaa gacctgtggt tttactaaag
    16201 gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag
    16261 ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg
    16321 gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg
    16381 taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac
    16441 tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg
    16501 tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag
    16561 cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc
    16621 cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg
    16681 tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga
    16741 tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta
    16801 ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc
    16861 aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat
    16921 tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg
    16981 aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg
    17041 acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggttatc ccgacaggct
    17101 actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc
    17161 aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc
    17221 tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc
    17281 tctctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct
    17341 ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg
    17401 ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg
    17461 cgccggatgg caaaacgcac gaattatcct cttaatctca ccacttatgc ccgcactccc
    17521 ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt
    17581 gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa
    17641 gtgtttttaa cgtcatttag actgaccgac aacgggcagt ttttttatta ccaggatagt
    17701 tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg
    17761 atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag
    17821 gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat
    17881 cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc
    17941 ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca
    18001 caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt
    18061 gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg
    18121 aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc
    18181 atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag
    18241 ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg
    18301 ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg
    18361 gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc
    18421 aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt
    18481 tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta
    18541 tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac
    18601 tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc
    18661 agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag
    18721 tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg
    18781 cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg
    18841 gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat
    18901 attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag
    18961 cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag
    19021 gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag
    19081 ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc
    19141 gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca
    19201 cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag
    19261 ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc
    19321 gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg
    19381 cgtttcgccg cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg
    19441 aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga
    19501 acatcaacta acgcttcacc agcaatggac accaggcaag aatccgcccc gcgtttcacc
    19561 accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga
    19621 ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt
    19681 tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg
    19741 tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg
    19801 cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc
    19861 gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct
    19921 tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg
    19981 cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag
    20041 gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt
    20101 aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg
    20161 aaaccgcgct taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg
    20221 gcaatctttt tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct
    20281 gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac
    20341 gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt
    20401 ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga
    20461 catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga
    20521 gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct
    20581 atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga
    20641 acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga
    20701 tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc
    20761 acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac
    20821 caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata
    20881 tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg
    20941 atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca
    21001 tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct
    21061 tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag
    21121 aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt
    21181 ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg
    21241 cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc
    21301 gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga
    21361 ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca
    21421 accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcaat ctgggcgtgg
    21481 tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac
    21541 atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt
    21601 tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc
    21661 cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa aagaacaata
    21721 actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat
    21781 ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag
    21841 taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg ccattcagcg
    21901 aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac atttttggcc
    21961 tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa ataggcggta
    22021 tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta
    22081 atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt
    22141 tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc
    22201 tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa
    22261 aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg
    22321 gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc
    22381 ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag
    22441 aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag
    22501 tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct
    22561 tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata
    22621 tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca
    22681 gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata
    22741 ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga
    22801 cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc
    22861 ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca
    22921 acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg
    22981 acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc
    23041 gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa
    23101 gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta
    23161 tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga
    23221 aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg ctggtaacac
    23281 cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga
    23341 cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat ccggcaatta
    23401 aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct
    23461 tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag tcctgataag
    23521 cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct catgggtcag
    23581 cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa
    23641 cccgtagtta gcctgcaacc aggctgcgat atacggcgca acggaagccc cgagaatcga
    23701 cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa acagctctgg
    23761 taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca gcagcaggaa
    23821 ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa
    23881 caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg catcagccag
    23941 taatccggcg actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa
    24001 cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga
    24061 gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac
    24121 ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc
    24181 tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat
    24241 aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc
    24301 atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg
    24361 tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt
    24421 cgccagcagc gccgcaccgc cccattcacc gcctaagccc agaccctgac caaatcgagc
    24481 cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat
    24541 caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac
    24601 gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc
    24661 gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg
    24721 cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt
    24781 gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgttc
    24841 gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata
    24901 gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa
    24961 aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca
    25021 cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga
    25081 tttatatgct ggggttatga gttggagtga aattgtttgc tcccaaatag atggctgaag
    25141 aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc
    25201 gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt
    25261 tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta
    25321 tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa
    25381 aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat
    25441 ggcagggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt
    25501 ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag
    25561 aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc
    25621 agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca
    25681 accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa
    25741 tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg
    25801 cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt
    25861 gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg
    25921 tttttcaacg tggcggctag tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata
    25981 tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc
    26041 aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc
    26101 agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg
    26161 actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg
    26221 ctattatcca actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg
    26281 ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc
    26341 ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc
    26401 cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag
    26461 cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg
    26521 atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc
    26581 gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct
    26641 aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg
    26701 gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt
    26761 agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg
    26821 caatcaaaac catcatgaag aatatctacc ggacggttat ggctactcag ttccagctca
    26881 attcccggat gctgatagag aaattccgac agacgcggca gtaacaggtt tttcgcgatt
    26941 cctgacggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga
    27001 ccgtccagtt cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct
    27061 tctggcgtga gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt
    27121 tccagtgcct gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg
    27181 cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc
    27241 atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt
    27301 gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc
    27361 tcagcaggaa aagggcggaa tagctttatg agaaaggtga atccccttaa acagagcgta
    27421 tttttcagtg attcccttta aagtgaatag aggacgtatt ggctatttgt gcttattttt
    27481 gtctcattgc catttgttat tttggcttgt agtcgcggaa tttcattttt aattgcatgc
    27541 gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt
    27601 aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct
    27661 gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca
    27721 ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg
    27781 acaggcagtc aatatttatt catggaataa aaatcagttt acagcagcgt attccaggag
    27841 tgagtattca gggggtcagt caggcagacg agttatggca aaagctggaa agttaccctg
    27901 aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa
    27961 aaaccgtggt gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac
    28021 ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgc gattcaaccg
    28081 tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag
    28141 actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc
    28201 gggagatttt aaccatgtta gcggcgggcg aatcaaataa ggagatcggc agagcgctga
    28261 atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga
    28321 aaaatcgcac ccaggcggca atgatgttaa atatctcctc ctg
//
LOCUS       DAJTNW010000056        28021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNW010000056 DAJTNW010000000
VERSION     DAJTNW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="OO179_002422"
                     /pseudo
     CDS             complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="OO179_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1001..2023)
                     /locus_tag="OO179_002423"
     CDS             complement(1001..2023)
                     /locus_tag="OO179_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5532450.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2037..3539)
                     /locus_tag="OO179_002424"
     CDS             complement(2037..3539)
                     /locus_tag="OO179_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5532451.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3679..4635)
                     /gene="ytfQ"
                     /locus_tag="OO179_002425"
                     /pseudo
     CDS             complement(3679..4635)
                     /gene="ytfQ"
                     /locus_tag="OO179_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4945..5475
                     /gene="ppa"
                     /locus_tag="OO179_002426"
     CDS             4945..5475
                     /gene="ppa"
                     /locus_tag="OO179_002426"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5532452.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5854..6195)
                     /locus_tag="OO179_002427"
     CDS             complement(5854..6195)
                     /locus_tag="OO179_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5532453.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6198..9977)
                     /gene="tamB"
                     /locus_tag="OO179_002428"
     CDS             complement(6198..9977)
                     /gene="tamB"
                     /locus_tag="OO179_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5532454.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9974..11707)
                     /gene="tamA"
                     /locus_tag="OO179_002429"
     CDS             complement(9974..11707)
                     /gene="tamA"
                     /locus_tag="OO179_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5532455.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11913..12551
                     /gene="msrA"
                     /locus_tag="OO179_002430"
     CDS             11913..12551
                     /gene="msrA"
                     /locus_tag="OO179_002430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5532456.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12874..14217
                     /locus_tag="OO179_002431"
     CDS             12874..14217
                     /locus_tag="OO179_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5532457.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14279..14485)
                     /locus_tag="OO179_002432"
     CDS             complement(14279..14485)
                     /locus_tag="OO179_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5532458.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14810..15364
                     /locus_tag="OO179_002433"
     CDS             14810..15364
                     /locus_tag="OO179_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5532459.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15427..16365)
                     /locus_tag="OO179_002434"
     CDS             complement(15427..16365)
                     /locus_tag="OO179_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532460.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16577..17317)
                     /gene="cysQ"
                     /locus_tag="OO179_002435"
     CDS             complement(16577..17317)
                     /gene="cysQ"
                     /locus_tag="OO179_002435"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5532461.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17507..19450
                     /gene="cpdB"
                     /locus_tag="OO179_002436"
     CDS             17507..19450
                     /gene="cpdB"
                     /locus_tag="OO179_002436"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5532462.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19576..19956)
                     /locus_tag="OO179_002437"
     CDS             complement(19576..19956)
                     /locus_tag="OO179_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5532463.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20045..20905
                     /gene="qorB"
                     /locus_tag="OO179_002438"
     CDS             20045..20905
                     /gene="qorB"
                     /locus_tag="OO179_002438"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5532464.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21014..21979
                     /locus_tag="OO179_002439"
     CDS             21014..21979
                     /locus_tag="OO179_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5532465.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22087..22749
                     /gene="ytfE"
                     /locus_tag="OO179_002440"
     CDS             22087..22749
                     /gene="ytfE"
                     /locus_tag="OO179_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5532466.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22794..24206)
                     /gene="cycA"
                     /locus_tag="OO179_002441"
                     /gene_synonym="dagA"
     CDS             complement(22794..24206)
                     /gene="cycA"
                     /locus_tag="OO179_002441"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5532467.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24514..25134)
                     /gene="fklB"
                     /locus_tag="OO179_002442"
     CDS             complement(24514..25134)
                     /gene="fklB"
                     /locus_tag="OO179_002442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5532468.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25353..25898
                     /locus_tag="OO179_002443"
     CDS             25353..25898
                     /locus_tag="OO179_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5532469.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25882..26529)
                     /locus_tag="OO179_002444"
     CDS             complement(25882..26529)
                     /locus_tag="OO179_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5532470.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26741..27532
                     /locus_tag="OO179_002445"
     CDS             26741..27532
                     /locus_tag="OO179_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5532471.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27542..>28021
                     /locus_tag="OO179_002446"
     CDS             27542..>28021
                     /locus_tag="OO179_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5532472.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     "
BASE COUNT         6888 a         7470 c         6895 g         6768 t
ORIGIN      
        1 ccacacggag aggcattttt atcgctgcgc aatgttgact cttgtcactg gcgaactctg
       61 acgattctcc cacagcaccg ttaacccacg ctgtaatgct ataaaaataa acaacaaaat
      121 accgatggcg attttcgtcc actaggaact cagcgtgccg tcaaagttga tgtaagtctg
      181 gatcagtccc tgaattgcca cgccaaaaag cgtcccaagc accgtcccga cgccaccact
      241 taacaacgta ccgccaatca ccaccgacgc gatggcgtcc agttccacac ctacgcccgc
      301 cagtgcatat ccggcttggg tataaatcga gaagacaatc cccgccagtg ttgccagtcc
      361 ggtggagagc atataaatgc gaatagtggt gctgcgagta gaaatcccca tcaggttcgc
      421 cgacgtcgcg ttgccgccaa tagcgtacac ctgattgcca aaacgggtac aatgcgcaag
      481 gaaaataccg ataaccacca ccgccagcat caacagcccc atcgcactaa ggcgaccgcc
      541 gccggggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcggaat
      601 cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacatccccg caagcgtaat
      661 gataaatgcc gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca
      721 gcccatcacc agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaaccac
      781 ttttgccaga aacacgccag taaaggcgat caccgaaccg acggagagat cgataccgcc
      841 agagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcgt tatcggtcag
      901 gatattgcag atcacccttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
      961 gcccagcaca aagacgccga tggtgatcat cagcggcaag ttacgtttta tcatggctac
     1021 gtactccttt aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca
     1081 ccaccgcttt caccacctgg ttcatctccg gcggaaagcc cgaaagcaaa attccggtgt
     1141 tcatcccctg aataatcagc gcccccacta ccgagagcag caggttaaag cgcccgccca
     1201 tcagcgatcc gccgccaatc accaccgcga gaatggcgtc cagctccagc cataatccgg
     1261 cgttgttggc atcggcaccg cgaatatccg ccgccacgat aatgcccgca atcgccgcac
     1321 atagcccgct caacacgtag gtgagcatga cgatgattcg cgtgtttacc ccggcatttt
     1381 ttgccgcccg aatgttgata ccaacgactt cgataaacat ccccagcgcc gttttgcggg
     1441 tcaacagcca gaacaggaga agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca
     1501 acagcgatcc gctgccaaac catgagagat ccggcgagtt aaacgtgacg atctgcccgg
     1561 cggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg
     1621 gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccggtgc
     1681 ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg
     1741 tggctccggc gatagccatc accgccccca cagagagatc gataccaccg gtggcgatca
     1801 ccagcgtcat accaatcgcc agtaacgcaa cgggagctgc acggttaaga atgtcgatag
     1861 ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg gctaccaggc
     1921 tatcgaccag cagcaccagc aacagtgccg ccagctgcgg catccccgtc ggccagtgaa
     1981 aacgcctttt tggcggcgta gtgtcaggga gagattgagg catcacactg ttctccttac
     2041 gccgcaatgg cgttcatgat cgccggaacg gaaagctgtg ccagcgggat ctccgccacc
     2101 tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc ccaccagctc ttccagttcg
     2161 gaggagatca ccagcagcgc cagaccgtca gcacacagcg tttcaatcag gcggatgatc
     2221 tcggcgtggg caccaacatc aatgccacgg gttagctcat caaggatcag aaattgcggt
     2281 cgggtcagta gccaacgtga aagcagcact ttttgctgat tgccaccgga gagaaattca
     2341 atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc
     2401 tcttgctgct ctttgcggga aatgggtcgt agccagccgc gctgggcctg gagagcgagg
     2461 atgatatttt cccgcaccga ggcggcggcg atgatgccat cggttttcct gtcttccggg
     2521 cagaatccga tgcccagtac tgaagcctga tgtggcgatc gcagggtttg cggtttgcct
     2581 ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg
     2641 gtacgtccgg atcccagcaa gccagccaga ccgacgatct cgccggggcg cacttcgaga
     2701 tcaaacggtg ctatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtcg
     2761 ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc
     2821 atttttacca gttcgatctg cggtagctcg cgcgtttccc gacagcctac gaaactgccg
     2881 ttgcgtaaga cggtgatccg atcgctgacc tgatagacct gatcgagaaa gtgagtgaca
     2941 aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggccaaataa tagctccatt
     3001 tcctgggtgt cgaggctggc ggtgggttca tcgaggatca gcactttggc ggagagatcg
     3061 atagcccggc aaatggcgac gatttgctgc atcgccaccg aaaaacggtt aagcggttcg
     3121 cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct
     3181 ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt atcagcaacc
     3241 gacatattgg gtagcaggtt gacttcctga tagacggtgc cgataccgag ttgttgtgca
     3301 tgggcggtat ttttcggtga gatagtctgg ccttccagcc agatggtgcc gcgatcggcg
     3361 tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccaagc
     3421 agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg
     3481 gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc ggtcgtcatg
     3541 gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat
     3601 caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg
     3661 catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc
     3721 ggtatcaggc aggtaaaggg tggatttggt taacgtcagc ttttcaggca tggtgccgtc
     3781 ttttttgtat ttctccagcg catcgaaagc gggtcccgcc atgttcggcg tcagttcaac
     3841 actggcgttc gcttcgccat ccatcatcgc tttgtagatg tccggtacac cgtcaatgga
     3901 acccgtgagg atatctttgc ccggtttcag gcccgcttct ttaattgcct gaattgcacc
     3961 aatcaccatg tcgtcgttat gggcgtaaac catgcagatg tttttgctgt tgttttccgc
     4021 tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga
     4081 gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttacggtc
     4141 aatggcgacg ctggccccaa cggtgccctg cagctccacc acgttgcatg gtttgccatt
     4201 cacttctttt accagccagt caccgatcaa cttaccttcg aggatgttgt cggcagtaac
     4261 ggtggtcata tagagagatt tgtctttcac atcgatggaa cggtcgagca agaagaccgg
     4321 gatttcggca tctttcgcct cttttaatac cggttcccaa cccgtcgcaa ccactggagc
     4381 gataaagatc gcatccaccc cttatgcaac gaaggaacgt accgctttaa tctggttttc
     4441 ctgcttttgc tgaccatcgg caattttcaa cgtaattccg cgtttttcgg cctcactttt
     4501 cgccacattg gtttctgcgg cgcgccagcc ggattccgat ccgacctgcg aaaatcctac
     4561 ggttaatgga gcggccaacg ccatagacga catggctgcc gagactgcag agactacaag
     4621 taagcgtttc cacatagggt tgtcctcgtc ggggtaatta ttattggtga aaagatgttc
     4681 gcgaaaaaac tatagacaat tcgttatgta acggattgcg ttacatcaca cttcaaaaaa
     4741 gtaaataaaa cgttaatcac aagtttgtaa tcgctttcat ctcactatga aaaatgcggc
     4801 tacggttatg gattttcctg ctctgtatac cgtcttaaaa ctggcgaaaa aggaaaatga
     4861 agacgaaaac aagcaaagac attcggcgcg agttggctat aatacttggc acttgtttgc
     4921 cacatatttt taaaggaaac agacatgagc ttactcaacg tccctgcggg taaagatctg
     4981 ccggaagaca tctacgttgt tattgagatc ccggctaacg cagatccgat caaatacgaa
     5041 atcgacaaag agagcggcgc actgttcgtt gaccgcttca tgtccaccgc gatgttctat
     5101 ccgtgcaact acggttacat caaccacacc ctgtctctgg acggtgaccc ggttgacgta
     5161 ctggtcccga ctccgtaccc gctgcagcct ggttctgtga tccgttgccg tccggttgcc
     5221 gttctgaaaa tgaccgacga agccggtgaa gatgcgaaac tggttgcggt tccgcacagc
     5281 aagctgagca aagaatacga tcacattaaa gacgtaaacg atctgcctga actgctgaaa
     5341 gcgcaaatcg ctcacttctt cgagcactac aaagacctcg aaaaaggcaa gtgggtgaaa
     5401 gttgaaggtt gggaaaacgc agaagccgct aaagctgaaa tcgttgcttc cttcgagcgc
     5461 gcaaagaata aataagttct tctagcgcaa taaccctgaa cgccgggctt cggttagtaa
     5521 gggttttttt atgcccgcga taaataaact ctctattcca ccatcattat tctcagcggt
     5581 tgcaaggctt gaacggtaag aacaagcaaa cccgaccacc attttgctgt tcatagccac
     5641 ttgctggaag ttagccgacc tcactcatac tcaccgttca aagcgtgccc ccgttttacc
     5701 cctgcacatt tgtgaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggct
     5761 cggagagaaa agcaaaagat ggaaaagtta caaaaaaaat cgcgagatac atcgcataaa
     5821 acaacgccac ccgaaggagg cgtgctttta ttattactta tccctgtcta accagtcgcc
     5881 gctttcaatt agctttaatc catcgacggg tcgttgataa acgtacatcc atgcactccc
     5941 atacggcgtc tgaatcaact ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag
     6001 ttcggccagc gtggcgttgt caatacgata aacttcaccg tgtaccgttc cgttccccgg
     6061 aactgcgcct ggatagtggc ccaggctata caactggtag ttatcgatac tgaaatcgcc
     6121 cagtaactgg gcattggtca tccagtgact gttgccttgt ttgtggcgta aactgccgta
     6181 gacaaatatt cgcattgcta aaactcgaac tgatagagca aatccagtgc ctggtctaca
     6241 ccagacacgg cttccagata tagcttaggc atcaggcgat aacgtaacgt gagtgttgct
     6301 atagagtcaa atatacccac gccgtatttc acttgcagac ctggcaatac atagccgctg
     6361 accactacct gggaggagtc gcctactccc agggtgtcga gcgctaaatt gcttacgcca
     6421 aacgtctcgc cgattttacc cacaatctgg ccactttgcg caacccccag accaatcagc
     6481 atcgaggtca ttgccgcact gtcgctctga tcgctctcca gcccttgtcc acgtagcaaa
     6541 taagacaatg cagcttgttg cgacatcgcc gggtcagaga agatctccgc tttcggttcg
     6601 tccgccagac cagtgacgcg aactccggcg attacatcgt cttctgtagc atccgggtta
     6661 cgaatagctt caatattaag atacggttga tccggcggac cagagaacag taactcaccc
     6721 ttacgcacaa tcagatcctg accataggca tggaagcgcc cttcagggat gttgatctgc
     6781 ccgttcaggc ccagcccttg tttgtcctgt acgacgttaa gatcgcccgt cagccgcgct
     6841 ttcaggccaa aggcgtcaat gcgcacattg ttgccaacgt ggacaatcag gttactgtta
     6901 atcggaatcg acgccgtttt cggctcttcc ggttgcaggt tatcgttaag catcaccaca
     6961 tcgctggaga cgcctactgc gctttccggc agatcgtgca ccacgatgcg cgcccacggg
     7021 acatccacgc gaccatcgag ggtaaacagg tttggtgtag cctcgaatac aacatctggc
     7081 gatacatcca ttcgtaccat cggcggcacg gtgatccgca ctttactgcc tttcgccgtt
     7141 actcgtgccc gccagttttc aatttggctc cagtcggcat taccgttcag gtagatttca
     7201 ccctgctggg tccgtactgt accggcaagc gtcgagcgca taccgttaaa gttgaccgca
     7261 agctggctcg gctgcatatc aaacggcata aagttgccgt cgatatccac acccgtaacc
     7321 tgaagctgac caaacaactg cgggctttgc acatcaccac ccagacgcaa gttggcactc
     7381 accatccccg ctgctttttc cccacgggta aagatggggt ttatcatcgc aaggttgaag
     7441 ttacggatat tgacgttgcc accaagatta cggcggcctt gcggatcggt cacctgcacc
     7501 tgtccatcaa actggccgtt attggtcagg cggatggtcc agcccaattc ggcacggttg
     7561 ttacgcaatt ccgccgtcag attcagtgtc tgaaacgcca ccggcagcgc cgcatcgttg
     7621 acggtttgcg ttacctgcac gttacgcccc gaaagggtga tactgccctg cggcagcccc
     7681 tctttcgtgg tgtcccaggc aacatccgct ttacccgtga agataccgct ggcctgagtg
     7741 gtttctggca taaacggttt cagcatggcg aggtcgaagc ggttgagatt caccaccgca
     7801 cgcccttcgg cccccgcatc gatagtttgc ggcacgcaca gttccgcatt cgggttaagc
     7861 cagcagtgtg gcccgatgct gattttttgc tccttattgc ggtaatccag cgcaatatcg
     7921 cgggtcagcg accacgggcc gaccggcgtc tggaagcggg tattgctaag agttcccttc
     7981 cagcgttctt ctttgcgatc aaaacttcct gccagattaa gctgcccgga gacaggctcg
     8041 ccctgaatcc gcaactgtag ctcgtgctgc ttttcgctgc ctttggcatt cagggtgacg
     8101 aggttgatat ttacatccgg ctgcgaaatt tgctcaacgc gtacgtcgag tttcccggcg
     8161 atctgatcgg tggatttaat gtcgccttcc acgcgaacct gcgccacgga aagttcctgc
     8221 cagcgcaggc cgcgcgcggt gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt
     8281 actttcacca gccctttcgc tgtaccgcca agccccggca gcgcgttatc cagccccggc
     8341 gcgttgatgg tggcatcaag attgagatct tttaccccca gctcgccttt cacttcggca
     8401 ctgtttggcc cgagttccag atgaagccca gggatcatcc actgcatata actgttgcct
     8461 ttcagcgtgc cgtcaacgtt cactttgttc tgtttgacgt taccggtcag cttcagttct
     8521 ggcacctcca tctgccaggt gccgccgtac aggctaccgc gggttttaat caagccattg
     8581 agtttcgacg gccactccgg gaactctttg gcggtgttaa tgccattaag cgttagctca
     8641 ccgcgccaac taatggcctg ctgccagtcg agcaacgcct tgagttcagt tttcccttcc
     8701 agcgccgcga cggtgagttt gtcgagattg acctgctgtt cattaccttt ggcatcaagg
     8761 gtaatggtgg ctggcgggat ctcctgtccc ttcactgccg tacgcataga gagcgtgtaa
     8821 tcggtcattt tgccggtaag tttcagtttc agatcatccg cctgatactg cttctcacca
     8881 gtgaacggcc agtaaagctg tttgctgttc acttccacgt tgagtggcaa tccggcttcc
     8941 gccagtcgcg tctgggcgcg taaatccata tccaccggac cggaaaggtt aacgcctatc
     9001 tccagctgtt cgcgcagcgc gccgcccact ttcagcttca ccttttcacc tttcaacggc
     9061 tccacgttca gtgtgctgtt gagggtgata tccaccggcc agttgtctga cagctgcgcc
     9121 gtgccgctgg cgttgacgat cccctggttg gaatcgatat ccagggcgtc cagtttagta
     9181 ttgccgtcaa tgctgctcac tttcagcagc atggtgcgca cggtgatgtc cgtgtcgccc
     9241 gtcacgcgca gctgctcgcc cttaaactct tcaatgttca ggttaagcgg caaatgcacg
     9301 tcggtcattt ccggcaatac cgggcgagaa aagagatctt tcagcgtttc gccgagcggc
     9361 ttttcctccg gctgcggatt ttcaattttc ggttcgacca cttcttcctg cgccacttcc
     9421 accactttcg gcagagcaat cagcaggcct ttcagcgacg tcggtttcag ggtcagggtt
     9481 ttctcctgcc agttcaggcc ggaggtgaag tccatcaccg acaccgtggt gtcatcaatc
     9541 ttgatgttga cgttgtccag cgccacccgt gtcagggtga tgggatacgg cgtggagaga
     9601 tccagcggac cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg
     9661 tcgatgttga cctgaatgtc tttcagcgcc aggtcattaa tacaaacgct actgttccac
     9721 aggcactcaa gaccgacccc cagatgcaga ttacccgctt ttaccgccac gcctagctgc
     9781 tcataacgaa cgtcagacaa ggtgagatcg cgccagccgc cggtgacctt gccaatatcc
     9841 agtcctggca cccagcgatc cgccgcttta aatacaagat gcaggccgct ggtggtgccc
     9901 accagaaacg ccaccgatcc cagcaacagt aagataacga taaccacgcc gaggctgatt
     9961 tttttccata aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt
    10021 ctttatccgc gaccggtacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc
    10081 gtacgccgac cccggtaccg gttttaaagt cgctgcggcg aatatcgctt accgcttcgc
    10141 cactatcgac aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tattccagcg
    10201 atccggttat caacttcgag gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag
    10261 atttgtattt gtagccgcga atactgcgat cgcccccgac gaagaaacgc agatccggcg
    10321 gtactttgtc gaaatcaccg gtttcaatcc agcccagcgt gccgcgtgtc acaaagcgat
    10381 ggcgatcgta cagtgtgcgg atccagacgt gctgcgcctg gaaaacggag aaatcgacat
    10441 ctgaacccca ggccgtgttg gagtagtcga tagagtagcg ttgcgagtct ccccaggttg
    10501 gcatcaggcc accacgagaa cgcgtgcggc taatcatcac cccaggataa aacagcatcg
    10561 tggtgttggt aatttcaccc tgggtaaagt ggtcgaggct ccagcgcagg ttaatggcac
    10621 gctgccagcc gctggagaga tcccagtagc gagaagccac caacgtagtg gagtcagatt
    10681 cggtatcgtt caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagtg
    10741 gattcttcag cagcggcatt ttatagctga agtcgagggt ctgttccggc gcggaaatac
    10801 tggtactggt ggtcaaactg tgaccgtatg agttcatcca cggctttttc cacgtcgctt
    10861 tcacgcgcgg tcccacgtcc gtagagtaac cgaccccggt ttcgatggtg ttttctgttc
    10921 gcggcgaaac cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg
    10981 gagccaccac caccgagtta aaccagccgg tagcagaaag tcggcggttc agttccgcca
    11041 gatctttcga ttcatactca tcgccctctt taaacggcac cagattttgc aggtattcat
    11101 cgcggatttg tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat
    11161 aatcaatatc ccagaaggct ttatgcaggc cgagcgcaat gcccagctgc gctttggtaa
    11221 attcgctatc gaaataacct ttacgcaacg caatactggt taaggacttt ttgaaatttt
    11281 cataatcgcc ctggttcagt accgtaccaa tagccgggcg agtatcgagc aatttcaaat
    11341 agtctttatc ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg
    11401 gcacacctgg cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga
    11461 gatcaaattc aatggtcggc tggtaataac ccagcgcttt cagaccttca cggatggcat
    11521 catcgacgcg tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa
    11581 gctgcgcacg aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt
    11641 tcgcggcgac ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat
    11701 agcgcacatt ttctcctgaa tatccttttt tcctgcccct ggaacgccgt taaacggctt
    11761 aacaaaaatc cagtaatatg gattaaaaag cagactaaac cccaaatatt tcttatgttt
    11821 tactttagac ctattcacgg tggttattgt gtgcaaatac gcctcttgtt acaaccttga
    11881 ccccaatgac cgattttcgg gagagcgaca ccatgagttt atttgataaa aagcatctgg
    11941 tttcccccgc cgatgccctg cctggacgta acaccccgat gcccgtagcc acgctgcatg
    12001 cggtcaacgg tcactcaatg accaatgtac ctgacggaat ggagattgcc atttttgcga
    12061 tgggttgttt ctggggtgtg gagcgtctgt tctggcagtt acccggcgtt tacagcaccg
    12121 ccgcaggcta taccggcggc tatacgccaa atccgactta tcgggaagtg tgctccggtg
    12181 atacgggtca tgccgaagcg gtacgcattg tttacgatcc ttccgtcatc agctatgagc
    12241 agttgctaca ggtattttgg gagaatcacg atcccgccca gggaatgcgt cagggcaatg
    12301 accacggcac gcagtatcgt tcagcgattt atccgctgac cccagaacag gatgccgcag
    12361 ctcgcgccag tctggaacgt tttcaggcgg cgatgcttgc cgccgatgac gatcgtcaca
    12421 tcaccacgga aatcgctaac gccacaccgt tttattatgc cgaagatgac caccagcaat
    12481 atctgcataa aaacccatat ggttactgtg gaattggcgg aattggcgtc tgtctgccgc
    12541 cggaagcata gcgttacggg tacaaatgta gtttgttgat aaagtgctct ttgtctatgc
    12601 ctgatgcggc ttaaacgact taccaggcct acaaaatcgc ccaaattcaa tatattgcaa
    12661 ggactgcgta ggcctgatcg gcatagcgca tcaggcagta ttgcatttat cagcagtctg
    12721 aattttaacc ctctggcgac tttacagtac cttacgctat actagccact gaaaatgccg
    12781 gttcactttc ttcgaatcgg ctttcaatgt gtatttcaca caaattaatc aactttccct
    12841 tccgagggtc tggcctgaaa ggtcggataa gatatgttaa acagtatttt agtcatactc
    12901 tgcttgatcg ctgtaagtgc gttcttctcg atgtccgaga tctcgcttgc cgcctcacgc
    12961 aaaatcaaac ttaaactgct ggctgatgaa ggcaatataa atgcccaacg cgttctgaat
    13021 atgcaggaaa atcccggcat gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg
    13081 attctcggcg gtatcgtcgg tgatgcggca ttttctccag cttttcacag cctgttctcc
    13141 cgctatatgt cggcagagct ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg
    13201 actggcatgt ttatcctgtt tgcggattta accccgaaac gcatcggtat gattgcgcca
    13261 gaagcggtgg ctttgcgtat catcaacccg atgcgcttct gcctgtacgt ttgcaccccg
    13321 ctggtgtggt tcttcaacgg cctggcgaac atgatcttcc gtattttcaa actgccaatg
    13381 gtacgtaaag atgacatcac ttctgatgac atctacgcgg tagtggaagc cggtgcgctg
    13441 gcgggcgtgt tacgtaaaca ggaacacgag ctgattgaaa acgtctttga gctggaatcc
    13501 cgtaccgttc cgtcttcaat gacaccgcgt gaaaacgtga tttggtttga tctccacgaa
    13561 gatgagcaaa gcctgaagaa taaggtggcg gaacatccgc actctaagtt cctcgtctgt
    13621 aatgaagata ttgaccacat catcggttat gtcgattcta aagacctgct gaaccgcgtg
    13681 ctggctaacc aaagcctggc actgaacagc ggcgtacaaa ttcgcaacac gctgattgtg
    13741 ccggatacgt taaccctttc agaggcgttg gaaagtttta aaaccgcagg tgaagacttc
    13801 gcggtgatca tgaacgagta cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg
    13861 accacgctga tgggcgatct ggtcggtcag gggctggaag agcagattgt cgcccgtgat
    13921 gagaactcat ggctgattga cggcggcacc ccaattgacg acgtcatgcg cgtgctggat
    13981 attgacgagt tcccgcagtc gggcaactac gaaaccatcg gtggctttat gatgtttatg
    14041 ctgcgtaaga tcccgaaacg caccgattcg gtgaaattcg ccggctacaa atttgaagtg
    14101 gtggatatcg ataactaccg catcgaccag ctgctggtga cccggatcga cagcaaggcc
    14161 accgcccttt cgccaaaact gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc
    14221 ccagaaacat caacggctcc tgaatcagga gccgtcttat tactgcatag cactttggtt
    14281 aagccatctc tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat
    14341 gcagcttatc cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa
    14401 tgtccttgat gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg
    14461 ggttgtaccg ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctgtgcggtg
    14521 ccacaatcgg cccaactatt atgaggcgca aattttaatg cttagtgact atagactatc
    14581 cgggcaatgt ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt
    14641 atctttttac cgaagcgcgg tcttcagtat aagcattcat ttttcatatg atttgtgcgc
    14701 ttgaccgcaa actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa
    14761 ctcagaccca agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg
    14821 caagattctg gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga
    14881 aatcggtcag cgagtgccgc cgattggcat taccgatcgg ggcgagttgg tgcttgataa
    14941 agatcagttt agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact
    15001 gcaacatatt gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat
    15061 taaatcagcg aagttaccgc acgatcgtta ccagaccacc accattgtta acaccgacga
    15121 cgcgattccg ggttccggca tgtttgtgcg cagcagtctg gagagtaata aaaagcttta
    15181 tccctggtcg cagtttattg tcgatagcaa tggcgtcgcg cgcggtgcct ggcagctgga
    15241 tgaagaaagt tccgctgtag tggtgctgga taaagacggt cgcgtgcaat gggccaaaga
    15301 cggggcgctc acaccggaag aggtgcagca agtgatggac ctgctgcaaa agttgctcaa
    15361 ataatggcaa taacataaat tctggaaaaa taattttccc ctatcgttat ctatgggata
    15421 ggggaattaa gtctctgagt tcttacaccg aactaatact ggtaacgcta gttgctcttt
    15481 tttaaacgcc tcaatggtcg atttatattt ctcgcaaatc ttttctttgg taatattact
    15541 atctgcggcc agattcgcac ttactatcgc atccacttgt tcggctaatt cgacttgttg
    15601 gcaacctgca ttaaccagaa aattataaat aagagagtaa tcttcatcct ccaaaacaac
    15661 agaggaacca ttacgaaaac gttcatcccc tccatagatt ttaagcgcaa agcccaaaga
    15721 ttctatgact atggcaaaat tatcatcaat tattttttcg taaatatacc atccatcgca
    15781 ttctttaatt ccaccgcctg gtatttggag ttcaaattta ccccgtttta atttgcttac
    15841 tacatcctcc aataaggcat aaccgaaaga tacgtcacca aacgcctggc gaatataacc
    15901 tttaatcctt tttggtatgt cagactgttt tttctgcaaa atatcacagg catacaatct
    15961 tctgttttgt tctaacggaa actcgcggtt tttgaaatcc atgaatgagt tatactcatt
    16021 tattgttatg ggaatttctt cgtattcaaa cccactgcct cgcaatgtta ttgtatattc
    16081 agcaccgtta tctttaaggc tgaaactgat aagaaaatta tccatggcat taacagtttc
    16141 attttttgat gaaagcactt gataactatt tttatcaatc tgtgttgcgc aacgaaagga
    16201 ctcccgcaaa gttaacagac tatcataatt cgaaacccaa tcttttttct taaatgcatg
    16261 ctgaatatta ttaaaaaaat cgtttgaatg tgtttttgta tgcgatgact ttacaaataa
    16321 ctccaccgca ccaataaaat tattgtgtct gatcctgggt aacatatggc taccttattt
    16381 tatttggtta ttttatgtca ccattaaata tctcattata gaagtaaact atcaaaaaac
    16441 gctcattatt aaaatgtaac ttactagcaa ttgaattttt gcactttaaa tacaatctct
    16501 ttttatttat aactttttag gttgggataa atcaggatgg cagaatcagg aaatacactg
    16561 tttctgccat ctgaatttag taaatggaca ctctgaaccc cggattcagg aacgactcgc
    16621 gcggagtgta atccagcggt ttaccctgcc agtcgtgaac gtgcgctccg gcagctgcag
    16681 ccacagcatg tccggcggcg gtgtcccaaa tattcgttgg tccgaagcgc gggtacagct
    16741 gcgcctgtcc ttccgccacc aggcagaatt tcagcgaaga gccgatggac gtggtctgat
    16801 gttcgccaag ctgttgcaga tactctttca gctccgcatc tgcatgggaa cggctgataa
    16861 ccaccagcgg cgggcgcgca tcgcggacct gaatcagctt gcgcacaccg cactcttctt
    16921 tccaggcctt gccttctgcc gcgctgtaca ttacgttcat taccggcgca tacaccacac
    16981 ctaaaatcgg tttgccatgg tcaatgagcg caatgttaac ggtgaattcg ccattacgtt
    17041 taataaactc tttagtacca tccagcgggt ctaccagcca gtaacgctgc cagtgctgac
    17101 ggacttccca accgggagga tcttcttcag aaaggaccgg aatatccggt gccagcgtac
    17161 gtaaaccgtc catgataacg gtgtgagcgg caatatccgc tgccgttacc ggagaattgt
    17221 ccgctttgct gacgacgtcc atcggtttcg tcccgtcgta gacctgcata atggcatcgc
    17281 ctgcattccg tgcaagctgg catacttgat ctaacatttc tccacctcgt ctctgtgagc
    17341 ggtgttaact tattgtttta cttataccct atcgttaatg aatgcgccaa ctgtgatagt
    17401 gtcatcattt tcaaagcgta aaattgtggc attcttcact gttctataag taaggcgttt
    17461 attctccctt ttctttcgta ttcccgatga taaaaggatg tccctgatga ttaagtttag
    17521 cgcaacgctc ctggccacgc tgattgccgc cagtgtgaat gcagcgacgg tcgatctgcg
    17581 tatcatggaa accactgatc tgcatagcaa catgatggat ttcgattatt acaaagacac
    17641 cgccacggaa aaattcggac tggtacgtac ggcaagcctg attaacgatg cccgcaatga
    17701 agtgaaaaac agcgtactgg tcgataacgg cgatttgatt caggggagtc cgctggccga
    17761 ttacatgtcg gcgaaaggat taaaagcagg tgatgttcat ccggtttata aggcgctgaa
    17821 tacgctggat tatacggtcg gtacactcgg caatcatgaa tttaactacg gtctggatta
    17881 cctgaaaaat gcgctggcgg gagcgaaatt cccttatgta aatgccaacg tcattgacgc
    17941 cagaaccaaa cagccaatgt ttacaccgta tttaattaaa gataccgaag tggtcgataa
    18001 agacggaaaa aaacagacgc tgaagattgg ctatattggc gtcgtgccgc cgcaaatcat
    18061 gggctgggat aaagctaatt tatccggaaa agtgacggtg aatgatatta ccgaaaccgt
    18121 gcgcaaatac gtgcctgaaa tgcgcgagaa aggtgccgat gtcattgtcg ttctggcgca
    18181 ttccgggctg tctgccgatc cgtataaagt aatggcggaa aactcagttt attacctcag
    18241 tgaaattccg ggcgttaacg ccattatgtt tggccatgct cacgccgttt tcccgggtaa
    18301 agattttgct gatatcgaag gggctgatat caccaaaggc acgctgaatg gtgttccggc
    18361 ggtaatgccg ggcatgtggg gcgatcatct tggggtggtc gacttacaac tcagtaatga
    18421 cagcggtaaa tggcaggtga cgcaggcgaa agcggaagca cggccgattt acgacatcgc
    18481 caataaaaaa tccctcgcgg cggaagacag caagctggta gaaacactca aagccgatca
    18541 cgatgccaca cgccagttcg tcagcaagcc aatcggtaaa tctgccgaca atatgtatag
    18601 ctatctggcg ctggtgcagg acgatccgac cgtgcaagtc gtgaacaacg cgcaaaaagc
    18661 gtatgtcgaa cattacattc agggcgatcc ggatctggca aaactgccgg tgctttcagc
    18721 tgccgcaccg tttaaagttg gtggtcgcaa aaatgacccg gcaagctatg tggaggtgga
    18781 aaaaggccag ttgaccttcc gtaatgctgc cgatctttat ctctacccca atacgctgat
    18841 tgtagtgaaa gccagcggta aagaggtgaa agagtggctg gagtgctccg ccggacagtt
    18901 taaccagatt gctcctaaca gcacgaaacc acagtcactc atcaactggg atggtttccg
    18961 cacttataac tttgatgtta ttgatggtgt gaattatcag attgatgtta cccaacccgc
    19021 ccgctatgac ggcgagtgcc agatgattaa tgccaatgca gagcggatta agaacctgac
    19081 ctttaatggc aagccgattg atccgaacgc tatgttcctg gttgccacca ataactatcg
    19141 cgcttacggc ggcaaatttg ccggtacggg cgacagccat atcgcttttg cttcaccgga
    19201 tgagaaccgc tcggtgctgg cagcgtggat tgctgatgag tcgaaacgtg cgggggaaat
    19261 tcatccggcg gcagataaca actggcgttt agcaccgata gctgccgata agaaactgga
    19321 tatccgtttc gaaacttccc cgtcagataa agccgcagcg tttattaaag agaaagggca
    19381 gtatccgatg aataaagtcg cgaccgatga tatcgggttt gcgatttatc aggtggattt
    19441 gagtaagtaa aacacttctt tttcggccta taaatcaacc gcatccggca tttattggcg
    19501 gatgcgatgc tgccgtatct tatccaccct acaagccatg aaccgtagac cggataagcg
    19561 cagcgcatcc ggcagttatg ccgcacgttc atcccgcacc gccaacacct cgggcaaatt
    19621 caactcaatc cagtccgcca gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt
    19681 gaggctatat tccacatgcg gcggcaccac cggatacgcg atacggttaa gaaaaccatc
    19741 ctgttctaac gcctgtaacg actgcgcaag catcttttca ctcaccccac cgattttgcg
    19801 ccgcaggtcg ctaaagcgat gagtaccttc gcgtagcgcc accagaatca acacccccca
    19861 acggctggtg acgtgtttca acacctcgcg cgacgggcac tgttccgcaa agagattacc
    19921 ctctttcagt tgttgtgaca ggctaacctg actcatttca tacttacctt tttgcacgta
    19981 cttactaaaa gtaagtttag gtgttagcgt atttaaacac aagacaaaac gatggagact
    20041 tcccatgatc gctattactg gtgccactgg ccaacttggt cactatatta ttgaatcctt
    20101 gatgaaaacg gttcctgcca gccaaatagt ggctatcgtt cgtaatccgg caaaagccca
    20161 ggccctggca gcacaaggca ttaccgtgcg tcaggctgac tacagcgatg aagccgcact
    20221 gacatctgca cttcagggag tggaaaaact actgctgatc tcttccagcg aagtgggtca
    20281 acgtgccccg cagcatcgta atgttattaa tgccgcaaag gcggctggcg tgaaatttat
    20341 cgcttatacc agcctgctac atgcagatac ctccccgctc ggcctcgccg atgagcacat
    20401 cgagacggag aaaatgctgg ctgattctgg catcgtttac accctgctgc gcaacggctg
    20461 gtacagcgaa aactacctcg ccagcgcccc ggcagcactg gaacacggcg tatttatcgg
    20521 tgcggcgggc gatggcaaaa tcgcctcagc aacgcgggca gattatgcga cagctgcggc
    20581 acgcgtgatt agcgaagccg gtcacgaagg caaggtttac gaactggcgg gcgatagtgc
    20641 ctggacgttg acacagttag cggcagagct gaccaaacag agcggcaaac aggttaccta
    20701 tcaaaatctg agcgaagccg atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg
    20761 actggcggat atgctggcgg attctgacgt tggcgcatcg aaaggtggtc tgtttgataa
    20821 cagcaaaacg cttagcaaat tgattggctg cccaacgaca acgttagccg aaagcgtaag
    20881 ccatcttttt aatgttaata actagttaat taaagtggca tcctcccgca tcctctctga
    20941 taatgacggg atgccgggag caatcatgtc tgcttcctga acgtttcttc tgacagacca
    21001 atggatgcca gtaatgatta gcggcgtgct gtacgccctg ttagcagggt tgatgtgggg
    21061 gcttattttt gtcgggccgt tgatcgtgcc ggaatacccg gcgatgttgc agtcgatggg
    21121 gcgttatctg gcgttagggt taattgcgct acccattgcc tggctgggac gcgtgcgtct
    21181 gcgtcagttg gcgcgtcggg actggcttac cgccttgatg ctcactatga tgggcaacct
    21241 catttattac ttctgccttg ccagtgccat tcaacgtact ggcgcgcctg tttccacgat
    21301 gattatcggc accctgccgg tggtgattcc tgtctttgcc aatctgcttt atagccagcg
    21361 cgacggcaaa ctcgcgtggg gaaaactcgc cccggcactg atttgtattg gcatcggcct
    21421 ggcgagtgtg aatattgctg agttaaacca cggactcccc gattttgact gggcacgtta
    21481 tacctctggc atcgtgctag cgttagtttc cgtggtctgc tgggcatggt atgccctgcg
    21541 caacgcccgc tggctgcggg aaaatcccga caaacatccg atgatgtggg cgacggtgca
    21601 ggcgctggtc acgctgccgg tttctctcat cggctatctc gtcgcctgtt actggctgaa
    21661 tatacaaacg ccggacttct ccttaccttt tggcccccgt ccgctggtgt ttattagtct
    21721 gatggttgcg atagccgtgc tttgctcatg ggttggcgca ctctgctgga acgtcgccag
    21781 ccagcgatta ccgacagtga ttctcgggcc gctgattgtt ttcgaaacgc tggcaggttt
    21841 gctgtacacc tttttactcc gccagcaaat gccgccgcta atgacgctga gcggtatcgc
    21901 gctgttagtg attggcgtgg tcatcgcggt cagagcaaaa ccagaaaaac ctttaactga
    21961 atctgtctca gaaagttgac atgctggcag tgagttaaat aagcctctgc tacgtaaggg
    22021 ttatagcttt tgccttaaag gtgtatttaa aatacatctt atcttattaa gaatgaggta
    22081 tcagctatgg cttatcgcga ccaaccttta ggtgaactgg cgctctctat ccctcgcgct
    22141 tcggctctgt ttcgtaaata tgatatggat tactgctgtg gcggtaagca gacgctggcg
    22201 cgcgcggcgg cacgtaaaga actggatgtt gaggtcattg aagctgaact ggcaaagctt
    22261 gctgaacaac cgattgagaa agactggcgt agcgccccgc tggcagaaat cattgaccat
    22321 atcatcgtgc gctaccacga tcgtcaccgc gagcagctgc cggagctgat cctgcaagcg
    22381 actaaagtcg agcgcgttca cgccgacaaa ccgagcgtgc caaaagggct gacaaaatac
    22441 ctgaccatgc tgcatgaaga gctttccagc cacatgatga aagaagagca gatcctcttc
    22501 ccgatgatca aacaaggcat gggcagccag gcaatggggc caatcagcgt aatggaaagc
    22561 gagcacgatg aagcgggcga actgctggaa gtgatcaaac acactaccaa taacgtcaca
    22621 ccgccgccag aagcctgcac cacctggaaa gcgatgtata acggcattaa tgaactgatt
    22681 gatgacctga tggatcacat cagtctggaa aacaatgtac tgttcccacg cgccctagcg
    22741 ggtgagtgat ctaaaagctg gatggcattg cgccatccag catgataatg cggttatttc
    22801 cgcagttcag cagcccgctt cttaccaata aacagccagc ccaaccccag cgcgataaac
    22861 cacagcgggg taaccagcag cgcctggcga gtgtcatctt ccagtgtcag caacacgacc
    22921 acgaacacaa agaacgccat acatacccag cacatcagct tgccgagcgg catcttgtag
    22981 atcgactttt catgcagatg aggacgctgt ttgcggtaca ccaggtacga gcaaaggata
    23041 atcgtccaga cgaacataaa cagaatcgcg gaaacggttg taatcatcgt gaacgcgcca
    23101 atcacgctag gattcacata caacatcacc acgccgccga gcagacagat acacgagaag
    23161 gtcaaccctt tcgcgggtac tgcgcgctta gaaagtttag cgaacgcttt cggcgcaaca
    23221 ccttcctgcg ccagaccaaa cagcatacgg ctggtagaga agacaccgct gttagcggaa
    23281 gacgccgcag aggtcagcac cacaaagttg atcacgctgg cagccgcagg caaacctacc
    23341 aacacaaaca gctcaacgaa cgggcttttc tccgggacta ccgaactcca cggtgtcacg
    23401 gacataatca caatcagcgc gaagacgtag aacataatga tacggatcgg aatggagtta
    23461 atcgcgcgtg gcagtgattt ctccggatct ttggtttccg cagctgttgt acctaccagc
    23521 tcaatcccca cgaaagcgaa aaccgctatc tggaatccgg caaagaagcc acttaaacct
    23581 ttcgggaacc agccgccgtc attccacaaa tgcgcgaatg acgcttccac accggtcggt
    23641 gactgaaagt gcatcgccac catgaccagg ccgacgacaa tcagcgacac gatggcgacg
    23701 attttgatca tcgcaaacca gaactccatc tcaccgaaca ttttcacggt ggcaaggttg
    23761 agcgtcagca gcagcactat caccgccagc gaggcgaccc agtcggagag atcggggaac
    23821 cagaactgag cataagccgt gatcgccacc acgtctgcca taccggttac aacccagcag
    23881 aaccagtaag tccagccggt gaaatatccc gcccacggcc ccagtaaatc ggaagcgaag
    23941 tcactaaaag atttgtattc cagattcgaa agcagcaatt cccccattgc ccgcatcacg
    24001 aaaaagagca taaaaccaat gatcatataa acgaaaatga tcgacggccc ggcaaggcta
    24061 atcgttttgc cggaccccat aaacaaccca gtaccaatgg cacctccaat ggcaataagc
    24121 tgaacatgtc ggtttgtgag attgcgccgt agcgactgtt cagccggagc ctgatcatcg
    24181 gcaacgactt ttacctgatc taccatgttt tttcttcctg tacctgtctg tgttgttcag
    24241 gctctacggc ctttagtcag tctatgatac gacgaatctg tatcgggact catcgatatt
    24301 aggtaacaat acgcgggatg aatactaaga tttagatcta atgttaattt tatgtttaaa
    24361 gtgagtgtca tatcacggtg ataacgcgaa acagctcaca aaaatacacg cttacggggt
    24421 ctttgcaaca caaaatagcg ccgtttggca aactgaaggg tttattgctg aatgcctgct
    24481 cccctctggt tcgaggggaa cagaatgcgc tgcttagagg atttccagca gttcgacttc
    24541 aaacaccagg gtgctgaacg gagggatgga tgcacctgcg ccgcgctcgc catatgccag
    24601 ttcctgcggg atagtcagtt cccatttaga acctaccggc atcagagtca gtgcttcaat
    24661 ccagccaggg atcacgccat taaccgggaa ttcagcgggt tcaccacgag caacggagct
    24721 gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg
    24781 tgccggaatt gcgccttcac cctggttgat cacgcggaat tgcaggccag attcggtgct
    24841 attcacacct tcttttttgg cgttttcttc caggtatttc acgccttcag cagccatcgc
    24901 ctggaaacgc tgacgacgaa cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg
    24961 caccacatca accggcacag ccggatgttt gccttccagc gcatcggcaa tacctgcaac
    25021 cagtgcttct ggcagcagcc cttccaggcc agattcactc agttgttgcc ctacctgcaa
    25081 accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt
    25141 tcctttctta gggtataaaa gtagcgggca gcatatcagc cacacttctt tgggtaaaac
    25201 tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa caatagcaat atcccgtatg
    25261 aatataataa aggcgggttt accacggaca tcatagcgtt agcttattca taggctatga
    25321 ttgaggaaca agacgtggag caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa
    25381 ccctggagaa agtctggcac gcgccggaca attttcgctt catggacccg ctgccgccga
    25441 tgcatcgtcg ggggatcatt attgccgcca ttgtattggt ggtcggattt ctgcttccat
    25501 ctgatgatac gcccaacgcg tcggtggtga cgcgtgaagc gcagttagat attcagtcgc
    25561 aatcacaacc gccgacagaa gaacagccgc aaactaccca aacgcaacca ttccagccag
    25621 atagcggaat tgataatcaa tggcgctctt accgtgtcga acccggtaaa acgatggcgc
    25681 agctgttccg cgaccacgga ctgcccgcta ccgatgtcta tgcaatggcg caggtagaag
    25741 gcgcgggtaa gccgctgagt aatttgcaaa atgggcagat ggtgaaaatc cgccagaacg
    25801 ccagcggcgt ggtgacgggc ttaaccattg atacggggaa taatcaacag gtcctgttta
    25861 cccgccagcc ggatggcagt tttattcgtg cgcggtagat gaagggtcaa ccaaaagcca
    25921 gctaaggatt gtctctttga tgaccttacg tctggcttcc agtgcctggg gggaaaccat
    25981 actgatatga taaactaggc caagcgtatg ctggttgatc aaataatatc ccccaagcgc
    26041 agcaatatta atattcacct gcaatgggtc gatacctggc ttaaaaatat tgtatttctt
    26101 cccttcatcc aataaggatt ccattaactg caagtgagca tggttaattt ctaagagacg
    26161 ctgtgatttt gcataatgca cacctttact ttgattttca ctatgcacaa tttttaaaaa
    26221 ccaaggatta gcaatataat aatcccatgt aaaatcaata attttctcaa tggctttttg
    26281 tggcggaaga tctgagaaat caagggcctt ttctttttgt cgaatatcgt tccaggtata
    26341 ttccagcact tcgataaata aattttcttt attaccaaag tggtgataaa ccgtctgttt
    26401 actacaccct gctgccttta caatattatc tactctggcc ccttcataac catattcggc
    26461 aaattcatta accgcacata gcagcaattt ttccttcagg ctctgtacgt tgctggtatc
    26521 aatatacata acgcttcccc acgacctctt cgtggtgact atcataacct gatttaattt
    26581 cagcttcaga aaaaagggca aaaacttgta ctttcatcag gttatcaaat gtcctgtgat
    26641 cgccatcgca tatcaaccaa ctagatggtt gatcttttta tcctccagtc gcaagctaat
    26701 catcaccaag gaaacaaaat tcaaaaaaga gagctattgt atgaaatcca ctcgtcctgt
    26761 tgccgtgatt accggagcca ctcgcggtat tggaaagggc tgtgcgcagg agcttgctcg
    26821 tggcgggttt aacctgctga ttaacgatcg tcccgatgcc gacagcgtag aaaaactgca
    26881 tatcacacaa caagaatgtt tggcggaagg cgtggaagtg atttgcttcc ctgctgatgt
    26941 cggtgatcta tctctgcatg aggagatgct cgacgcggcg caaaaccagt gggggcggct
    27001 ggactgtctg ctcaataacg cggggatttc agtgaaaaaa cgcggtgacc tcctcgacct
    27061 cgaaccagac agcttcgatc aaaacattgc cattaacacc cgcgcgccgt tcttccttgc
    27121 tcaggcattc agtaagcgct tactggcaca gccgaaaccg gaagcggagc tgccgcaccg
    27181 ctcgattatt ttcgtcagtt ccatcaacgc catcatgctg gcgatgaatc gcggcgaata
    27241 caccattgcc aaaaccgccg tttctgccgc tgctcgtctg tttgctgctc gcctttgtaa
    27301 tgaacaaatt ggcgtttatg aagtgcgccc tggtctcatc aaaactgata tgactattcc
    27361 ggcaacggct tattacgacg aactgattgc caaaggtctg gtgccgtggg gacgttgggg
    27421 ttatccggct gatattgctt ccaccgtccg cgcgatggcg gaaggtaaac tgatttacac
    27481 ctgtggtcag gcggtcgcaa tcgacggcgg cttaagtatg ccgcgcttct gaggctgaat
    27541 catgagagat ctgttacaac gcccggattt attcagtatc aataccgcga cactgggcta
    27601 taaaacgccg ctacccgcca ttattgatgc ctgcgccgca cgcggtattg gcgcaattgc
    27661 cccctggcgc agggagttgc agggtgaaga tctccagcaa atcacccgcc agcttgccgc
    27721 cagtaacatg agcgtttccg gtttgtgccg tagcacttac tacaccgcgc cgacactggc
    27781 ggaacgcaaa ctggcgattg atgataaccg ccgggcgctg gacgacgcag cggtgcttaa
    27841 cgccgcctgt tatatgcagg tggttggcgg tctgccaacg ggcactaaag atctatatga
    27901 agcgcgagaa caagtgaaac aagggattcg ccagttgctg ccacattcga aagatgtcgg
    27961 cgtgccaatt gcgctggagc cactgcaccc gatgacagct gccgatcgct cttgcctgtg
    28021 c
//
LOCUS       DAJTNW010000057        27537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNW010000057 DAJTNW010000000
VERSION     DAJTNW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO179_002447"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO179_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5532473.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO179_002448"
     CDS             complement(1614..1937)
                     /locus_tag="OO179_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5532474.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO179_002449"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO179_002449"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5532475.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO179_002450"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO179_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5532476.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO179_002451"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO179_002451"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5532477.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO179_002452"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO179_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5532478.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO179_002453"
     CDS             7537..8574
                     /locus_tag="OO179_002453"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5532479.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO179_002454"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO179_002454"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5532480.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO179_002455"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO179_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5532481.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO179_002456"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO179_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5532482.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO179_002457"
     CDS             10745..11626
                     /locus_tag="OO179_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532483.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO179_002458"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO179_002458"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5532484.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO179_002459"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO179_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5532485.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO179_002460"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO179_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO179_002461"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO179_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5532486.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO179_002462"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO179_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5532487.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO179_002463"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO179_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5532488.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO179_002464"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO179_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5532489.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO179_002465"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO179_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5532490.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO179_002466"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO179_002466"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5532491.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO179_002467"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO179_002467"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5532492.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO179_002468"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO179_002468"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5532493.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO179_002469"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO179_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5532494.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO179_002470"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO179_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5532495.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO179_002471"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO179_002471"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5532496.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO179_002472"
     CDS             24893..25273
                     /locus_tag="OO179_002472"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5532497.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO179_002473"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO179_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5532498.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO179_002474"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO179_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5532499.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27537
                     /locus_tag="OO179_002475"
                     /pseudo
     CDS             26442..27537
                     /locus_tag="OO179_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6796 a         6855 c         7219 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaa
//
LOCUS       DAJTNW010000058        26733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNW010000058 DAJTNW010000000
VERSION     DAJTNW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..140
                     /locus_tag="OO179_002476"
     CDS             <1..140
                     /locus_tag="OO179_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5532500.1"
                     /translation="GEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNL
                     A"
     gene            complement(137..277)
                     /locus_tag="OO179_002477"
     CDS             complement(137..277)
                     /locus_tag="OO179_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5532501.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            519..812
                     /locus_tag="OO179_002478"
     CDS             519..812
                     /locus_tag="OO179_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5532502.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            884..1543
                     /locus_tag="OO179_002479"
     CDS             884..1543
                     /locus_tag="OO179_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5532503.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1635..2081
                     /locus_tag="OO179_002480"
     CDS             1635..2081
                     /locus_tag="OO179_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5532504.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2288..3188)
                     /gene="hlyE"
                     /locus_tag="OO179_002481"
                     /pseudo
     CDS             complement(2288..3188)
                     /gene="hlyE"
                     /locus_tag="OO179_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3561..3980
                     /locus_tag="OO179_002482"
     CDS             3561..3980
                     /locus_tag="OO179_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5532505.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3980..5248
                     /gene="umuC"
                     /locus_tag="OO179_002483"
     CDS             3980..5248
                     /gene="umuC"
                     /locus_tag="OO179_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5532506.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5294..5824)
                     /gene="dsbB"
                     /locus_tag="OO179_002484"
     CDS             complement(5294..5824)
                     /gene="dsbB"
                     /locus_tag="OO179_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5532507.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5970..7511)
                     /gene="nhaB"
                     /locus_tag="OO179_002485"
     CDS             complement(5970..7511)
                     /gene="nhaB"
                     /locus_tag="OO179_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5532508.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7732..8451
                     /gene="fadR"
                     /locus_tag="OO179_002486"
     CDS             7732..8451
                     /gene="fadR"
                     /locus_tag="OO179_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5532509.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8503..10035)
                     /locus_tag="OO179_002487"
     CDS             complement(8503..10035)
                     /locus_tag="OO179_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5532510.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10365..11663
                     /gene="dadA"
                     /locus_tag="OO179_002488"
     CDS             10365..11663
                     /gene="dadA"
                     /locus_tag="OO179_002488"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5532511.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11673..12743
                     /gene="dadX"
                     /locus_tag="OO179_002489"
     CDS             11673..12743
                     /gene="dadX"
                     /locus_tag="OO179_002489"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5532512.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12911..14325)
                     /locus_tag="OO179_002490"
                     /pseudo
     CDS             complement(12911..14325)
                     /locus_tag="OO179_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14936..16672)
                     /locus_tag="OO179_002491"
     CDS             complement(14936..16672)
                     /locus_tag="OO179_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5532513.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16767..17681)
                     /gene="ldcA"
                     /locus_tag="OO179_002492"
     CDS             complement(16767..17681)
                     /gene="ldcA"
                     /locus_tag="OO179_002492"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5532514.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17781..18392
                     /gene="emtA"
                     /locus_tag="OO179_002493"
     CDS             17781..18392
                     /gene="emtA"
                     /locus_tag="OO179_002493"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5532515.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18394..19128)
                     /locus_tag="OO179_002494"
     CDS             complement(18394..19128)
                     /locus_tag="OO179_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5532516.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19329..19583
                     /locus_tag="OO179_002495"
     CDS             19329..19583
                     /locus_tag="OO179_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5532517.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19633..21093)
                     /locus_tag="OO179_002496"
     CDS             complement(19633..21093)
                     /locus_tag="OO179_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5532518.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21030..21587)
                     /locus_tag="OO179_002497"
     CDS             complement(21030..21587)
                     /locus_tag="OO179_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5532519.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21613..22467)
                     /locus_tag="OO179_002498"
     CDS             complement(21613..22467)
                     /locus_tag="OO179_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5532520.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22467..23276)
                     /locus_tag="OO179_002499"
     CDS             complement(22467..23276)
                     /locus_tag="OO179_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5532521.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23286..24044)
                     /locus_tag="OO179_002500"
     CDS             complement(23286..24044)
                     /locus_tag="OO179_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5532522.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24041..25021)
                     /locus_tag="OO179_002501"
     CDS             complement(24041..25021)
                     /locus_tag="OO179_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5532523.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25021..26043)
                     /locus_tag="OO179_002502"
     CDS             complement(25021..26043)
                     /locus_tag="OO179_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5532524.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26392..>26733
                     /locus_tag="OO179_002503"
     CDS             26392..>26733
                     /locus_tag="OO179_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532525.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6728 a         6507 c         6899 g         6599 t
ORIGIN      
        1 atggagaaca ctttatcagc cgggaaataa tgcgggcaac ggtgtttaat tatatcgaat
       61 gtgattacaa tcggtggcgg cggcacagtt ggtgtggcgg cctcagtccg gaacaatttg
      121 aaaaccagaa cctcgcttag gcctgtgtcc atattacgtg ggtaggatca ctatggcctt
      181 tgctgaactc aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa
      241 gcagcaatac agccttactg atgctcttga ttttcattgg tagtctcctg ttcagaatga
      301 gtacgatgaa ctgttataat ataacaatcc cttacgggag aattccagtg gcttttctgc
      361 gttcggtaaa atcccggcgc ggcatttttt gccgggggga ttacttataa aggggcatgg
      421 actcaacaaa ccgcagaaat ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt
      481 agcttatgcc aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg atttatcgaa
      541 gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc
      601 ctgaggaact gatgaaaggt tttggtcagc ctcagttagc gatgattctg ccgttggatg
      661 ggcggaagaa actggtcaat gctgatattg aaaaggttaa actggcatta accgagcaag
      721 gttactattt gcagttaccg ccaccacccg aagatttgct gaagcaacat ctttccgtca
      781 tggggcagaa aacagacgac actaacaaat aaccgatatc cggcggtggc attatctttg
      841 tcggcgcggg ttttcatatc cacgataagg tgaggggaac gttatgtatc aacatcacaa
      901 ctggcaaggt gcgctgctgg attatccggt gagtaaagta gtctgtgttg gcagtaacta
      961 tgccaaacat attaaagaga tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa
     1021 accagaaacg gcactgtgcg atctgcggca gccattggcg atcccatccg atttcggttc
     1081 agttcatcat gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc aggcaacgga
     1141 agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg cttgatctga cattgcgtga
     1201 tgttcaggga aaaatgaaga aagccgggca gccgtgggaa aaggctaaag cgtttgataa
     1261 ctcttgtccg ctttccgggt ttatccccgc ggcggaattt accggcgatc cacaaaatac
     1321 aacgctgggc ctgagcgtaa acggcgaaaa acgccagcaa ggtacgactg cggacatgat
     1381 ccataaaatc gttccgttga tcgcttatat gagcaagttt tttaccctca aggccggtga
     1441 cgttgtgttg acaggcacgc ctgatggcgt cggcccgttg caaagcggtg atgagctgac
     1501 agtcactttc gatgggcatt ctttgacaac tcgcgttttg taatactttt tgccgccaga
     1561 aagcggcggc aaaacttgca tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa
     1621 tggcggaaca cctgatgagc gatgtacctt tctggcaaag taaaaccctg gacgaaatga
     1681 gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca gtgttgcttg cataaactga
     1741 tggatgaaga caccgacgaa atctacttca ctaacgtcgc ctgtcgccag ctcaatatta
     1801 aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat
     1861 taacccgtga aaatctgcca acattcgaat ggctgccaat gacctgcgct tatcggttgc
     1921 tggcggaagg taaagattta cctgcgtggc atccgctact tactggttcg aaagcggcaa
     1981 tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact
     2041 ggcaggatca tatcttaaat aaacctgact gggcgcagtg ataaatttaa tagatgctga
     2101 tgggggcttt cttaatatac gtctggatgc tcgccatttc gtagccctgt ttttgaaagc
     2161 gctctgtcat aatgagagtt acccgatacc ttattatcaa caggtaactc tcaatcttta
     2221 aaaaacgtct gtgtttttaa atgtgataaa ccttatacct tgagtacatg gagagaataa
     2281 tcgcttatca gacttcaggt acttcaaaga gtgtcttttt accgtgtctt ttctgatact
     2341 cattacaggt gttaatcatt tttttggccg ctgcttttag caaagaaagc attaaatcat
     2401 cataatcaac atagaatctg gttgtttcag tttccgtttt tatctccccg atggcggcta
     2461 tttcggtggt taatttcaat ttggcggcat cgatatcttt attcgcttgt ttaaccgtgt
     2521 tagacagggt ggtaaagaaa ctctgcacag attttaactt gttcttcaat tctggaatca
     2581 gtttcccttc aactacgccc gcagcaatag aataggaaat gattaaacca aatggaccgg
     2641 cgacgacatc ggctgcggca ccggcatacg cttccttcct gattttatct acctgtgact
     2701 ggaaatagct gcttttttct gaaaaatcat tggttaactg gctatctaac gccagcagtt
     2761 tcccggaagc gttgttgaaa ctttgtgagc ttaccagcag ggatttttgc gcttcattca
     2821 gcttcgtgat gccgtcatcc agtaccttaa tgaggggcgg atgctttctt ctcattgtac
     2881 tcatcaaata gcaaaatata cgctgcgagc aattgcgtcg caacaccaca ccattcatac
     2941 actgtttggg ttgcttcaaa atacttatcc tggctatcca taagtaaggt tttaatatcg
     3001 ccgactaaaa cggaggctgc ctgtgaatac tcctgtttaa agcgacttaa ctcttttatg
     3061 gtttcatcaa aggtctgcca ggggatgacc tgatcgagat atttattata aagatctaat
     3121 gctccatctg cggtttcgat tgcgttttta actacttcta ccgttttatc tgcaacgatt
     3181 tcagtcataa tcattcgcct ctttaaatat ataaattgta atgaaactcc tgttttacaa
     3241 ctattaataa attttacttc atctaattca tagttagccg ggcgggatgc gtcaatgtct
     3301 ttatttctat taatatgata aatatcaaac aatgtttaat gtcattatgg cgaatgcttc
     3361 tattctattt ttttgccggg tgatattttt catttctgct ggatgagcgt cgtcgccaga
     3421 aggccacgtg agcacaggat aagagaacga aaaatcagca gtctatgcag cgacaaatat
     3481 tgatagcctg aatcagtatt gatctgctgg caagaacaga ccactgtata taaaaacagt
     3541 ataacttcag gcagattatt atgttgttta tcaagcctgc ggatctccgc gaaattgtga
     3601 cttttccgct atttagcgat cttgttcagt gtggctttcc ttcaccggca gcagattacg
     3661 ttgaacagcg catcgatctg aatcaactgt tgatccagca tcccagcgcg acttacttcg
     3721 tcaaagcaag tggtgattct atgattgatg gtggaattag tgacggtgat ttactgattg
     3781 tcgatagcgc tattaccgcc agccatggtg atattgtcat cgctgctgtt gacggcgagt
     3841 ttacggtgaa aaaattgcaa ctacgcccga cggtacagct tattcccatg aacagtgcgt
     3901 actcgcccat taccatcagt agcgaagata cgctggatgt ctttggtgtg gtgatccacg
     3961 tcgttaaggc gatgcgctga tgtttgccct ctgtgatgta aacgcgtttt atgccagctg
     4021 tgagacggtg tttcgccctg atttatgggg taaaccggtg gttgtgctat cgaataatga
     4081 cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctg ggcgttaaaa tgggcgatcc
     4141 ctggttcaaa caaaaagatc tgtttcgtcg ctgtggtgtg gtttgcttta gcagcaatta
     4201 tgagctttac gcagacatga gcaatcgggt gatgtcgacg ctggaagaac tatcgccccg
     4261 cgtcgagatt tacagtattg atgaagcatt ctgcgatctg acaggtgtgc gtaattgtcg
     4321 cgatctgact gattttggca gagaaattcg cgcaacggtg ctacaacgta cccatcttac
     4381 tgttggtgtg gggatcgccc agaccaaaac gttggctaag cttgccaatc atgcggcaaa
     4441 aaaatggcag cggcagacgg gtggggtggt ggatttatca aatctggaac gccagcgtaa
     4501 attaatgtct gctctccccg tggatgacgt ctgggggatt ggacggcgga tcagcaaaaa
     4561 actggacgcg atggggatca aaaccgttct cgatttggcg gatacagata tccggtttat
     4621 ccgtaaacat tttaatgtca tgctcgaaag aacggtgcgt gaactgcgcg gcgaaccctg
     4681 tttgcaactg gaagagtttg caccgacgaa gcaggaaatt atctgttccc gctcgtttgg
     4741 tgaacgcatc acggattata cgtcgatgcg gcaggccatt tgtagttacg ctgcccgggc
     4801 ggcggaaaaa cttcgcagtg agcatcaata ttgtcggttt atctccacat ttattaagac
     4861 gtcaccattt gcgctcaatg aaccttatta cggcaatagc gcgtcggtaa aactgctgac
     4921 gcccactcag gacagcaggg atatcattaa cgctgctacg cgatctctgg atgccatctg
     4981 gcaagcgggc catcgctacc aaaaagcggg cgtgatgctg ggggatttct tcagtcaggg
     5041 agtcgcgcag ctcaatttat tcgatgacaa cgcaccgcgc cccgggagtg agcaattgat
     5101 ggcggtaatg gatactctga atgctaaaga gggcagagga acactctatt ttgccgggca
     5161 ggggatccaa caacaatggc agatgaagcg agctatgctt tcaccacgtt atacaacgcg
     5221 aagttctgat ttactgcggg tcaaataaat atagcggcag gaaaaaagcg ctcccgcagg
     5281 agcgccgaat ggattagcga ccgaacagat cacgtttttt cgctttaaac ggctgggaaa
     5341 tcaccaccag caccgcgaca atcaggtaag cgataaaaat accgagcagc cactgcggca
     5401 tttccatacc taaaaaatcc cactgacgct cggcgcaatc gccagaggcg acaaacactt
     5461 gcggcaccca tttatccagc ggcagccatt ccgggaaacg aaccataaaa tcacaggtgg
     5521 caaatggcga aggatagagc tgaagcatgg tgtgctcgta agttaactgc acaccgcgga
     5581 acgcactata caaccagata accatcgcta cataacgcag cggagttttc ggggcgatcg
     5641 cgccaatcag cgcagcaccc agaacgccga ataacgcgca gcgttcataa atacagagca
     5701 cgcaaggttt cagtaacatc acatgctgga accacagcgc cgtcagttcc agtgccagag
     5761 cagtaaacgc catcaacagc cacgcgcccc ggccttgtga acattggttc aaaaatcgca
     5821 acataatcat ttccctgcaa tatgcataga gtgcgcagtt taaaccaatt cattcgctgc
     5881 gccaccagga ggcagcaaaa ataggacgca ataggacaat tatcgggcga aaaggcaaac
     5941 cgggcattta tatgcccggt aagttgttat caaagcgttg ctatccagcc catttgcata
     6001 aaccattcgg ttacaggggc aagcgtaaac tcgacgcaga gcaagccgac gagtgtcagt
     6061 acgagggtgt aaggcagggc catccacacc atgcggccat aagagaggcg aatcaatggc
     6121 gcgagtgcag aggtcagcag gaacaggaac gcagcctgac cgttcggcgt agcgacagag
     6181 ggcagattgg taccggtatt aatggcgacc gccagcagct cgtattgctt caacgtgata
     6241 gcgccacttt ccattgccgc tttcgcttcg ttgatataaa tcgtccccac gaagacgtta
     6301 tccgaaatgg atgacagcag accgttgaaa atatagaaca gcgacagctg agcatgttca
     6361 gatgcctgca acacaaactg aataatcggc gaaaacagtt gttggtcgat aatcaccgcg
     6421 actaccgaga aaaacaccgt caacagtgcg gtgaatggca gagattcggt gaaggcttta
     6481 ccgatagaat gttcatcggt gacaccggtc aatgatgttg ccagaataat gactgacaaa
     6541 ccaatcaagc caacttccgc cagatgcaac gccagcgcag tcaccagcca gacgccaata
     6601 atcgcctgga caatcagacg gattttatcc tgacgggtac gctggtggcg gctttgatcg
     6661 tcaaactgtt gcaacacttc gcggactttc tccggcagcg tttcaccgta gccaaaccaa
     6721 cgcagcttct ctaccagcag gcaggttaac aggccacaaa tcagaaccgg aacggtcacc
     6781 ggcgacatgc gcaggaagaa atcgccaaaa tgccagccag ccgctttagc gatgatcagg
     6841 ttctgtggtt cgcccaccat ggtcattacg ccgcctaatg cggtgccgac accggcatgc
     6901 atcatcaggc tacgcagaaa gccacggaac tgttccagaa ccacgttgta atgcttgtcg
     6961 atatgactat cgtcttgcag gtcggtgtct tcggtacggg aagaggctac gcgatgataa
     7021 ataccataaa aaccgactgc aacgctgatc accaccgcca cgacggttaa ggcatcgagg
     7081 aacgcggaga ggaacgctgc cgccacgcaa aaagagagcg acagcagcat tttggagcga
     7141 atgcttaaca gcaaacgggt aaatatgaac agcaacagct gtttcataaa atagataccc
     7201 gccaccataa acatcaataa cagcaagact tcaagatttg ccgccacctc ttcacggacg
     7261 tgttccgcgc tggtcatgcc gatgaatacc gcttcgatag ccaacagacc accggggagt
     7321 agcgggtagc atttcagggc catcgccaga gtgaaaataa attccgctac cagcaaccag
     7381 cccgcgacga aagggctgat gaggaaaatt aacgggttta cgattaagaa aatgatgagg
     7441 gcgagtttgt accagtcggg ggactggccc aaaaaattgc gccatagcgc gcggccccag
     7501 gagatctcca tgatggtttc ccttacctta caaataatca atgatgtttt tatgtttaaa
     7561 cgcaaagctt aacggtcagg caggagtgag gcaagtcttg atagtcaagg ggaaagagat
     7621 gcggaaaatg aagccttgat ccctttttct tctttttgtc tgctatcagc gtagttagcc
     7681 ctctggtatg atgagtccaa ctttgttttg ctgtgttatg gaaatctcac tatggtcatt
     7741 aaggcgcaaa gcccggcggg tttcgcggaa gagtacatta ttgaaagtat ctggaataac
     7801 cgcttccctc ccgggactat tttgcccgca gaacgtgaac tttcagaatt aattggcgta
     7861 acgcgtacta cgttacgtga agtgttacag cgtctggcac gagatggctg gttgaccatt
     7921 caacatggca agccgacgaa ggtgaataat ttctgggaaa cttccggttt aaatatcctt
     7981 gaaacactgg cgcgactgga tcacgaaagt gtgccgcagc ttattgataa tttgctgtcg
     8041 gtgcgtacca atatttccac tatttttatt cgcaccgcgt ttcgtcagca tcccgataaa
     8101 gcgcaggaag tgctggctac cgctaatgaa gtggccgatc acgccgatgc ctttgccgag
     8161 ctggattaca acatattccg cggcctggcg tttgcttccg gcaacccgat ttacggtctg
     8221 atccttaacg ggatgaaagg gctgtatacg cgcattggtc gtcactattt cgccaatccg
     8281 gaagcgcgca gtctggcgct gggcttctac cacaaactgt cggcgttgtg cagtgaaggc
     8341 gcgcacgatc aggtgtacga aacagtgcgt cgctatgggc atgagagtgg cgagatttgg
     8401 caccggatgc agaaaaatct gccgggtgat ttagccattc aggggcgata atcccttccg
     8461 tttaaagagc aaccccctca aacgaggggt tttttgttgt ttttacagat ttcccattct
     8521 tggcgggcaa cgttccagca actcgatgct gccgtcttcg ttttgctgtt cgagcatcac
     8581 atcaaatccc cacaggcgat gcacatgctt caggacttct ttgcgccccc gatccagcgg
     8641 tgcgcgatta tgtggaatat aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt
     8701 ccagatctga atattcggct ccagattact taagttatat tgcgacgata accggttacg
     8761 gatctcccga taaccttctt cattatgaat agcggaaatc tccagataat tatgccgatc
     8821 atcatccagc acggtgaaga agcggaaatc acgcatcact ttcggtgaca gaaactggct
     8881 gataaaactc tcatctttga aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga
     8941 accggcgata tccgggaacc agtatttgtc ttcttccgtt ggcgactgac aaatccgttt
     9001 aatatcctgg aacatggcga acccgagggc atacgggttg atgccgctgt accacgggct
     9061 gttatagggg ggctggaaga ccacattggt gtggctgtgc aaaaactcca gcataaaacg
     9121 ttccgttact ttcccttcat catacagatg gttaaggatg gtatagtgcc agaaggtcgc
     9181 acagccttcg ttcatcacct gagtctgttt ttgcggataa aaatactggc tcaccttacg
     9241 cacaatacgc aggatttcac gctgccatga ttccagcagt ggcgcatttt tctccataaa
     9301 atagagcagg ttttcttgtg gttcggacgg atagcggcgc gcttcagcaa ccgttttctc
     9361 ttcctcgcgc ttcggcaggg tacgccagag catattgacc tgactttgta gatactcttc
     9421 gcgacttttc tgccgggctt tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg
     9481 gtccacgccg tagttcatca gcgcgtggca cgagtccaga agccgttcta cttcatcaac
     9541 gccataacgt tcttcgcact cggtaatata tttacgggca aaaatcagat aatcgacaat
     9601 cgaactggcg tcggtccaac tgcggaataa gcaattgttt ttaaagaaag agttatgtcc
     9661 atagcaggcg tgtgccatca ccagcgcctg catagtaatg gtgttctctt ccatcaagta
     9721 agcgatacac gggttagagt taatgacgat ttcataggcc agcccttgct gaccgtgctt
     9781 atacagccgt tcagtctcga taaacttttt accgaatgac cagtgcggat agttaattgg
     9841 catgccgacg ctggaatagg catccatcat ctgttctgag gttatcactt caatctggtg
     9901 cgggtaggta tccagccggt agagtttcgc cacccggtct atctctgcca gataaacatc
     9961 cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt gtggtgtcct tattcataga
    10021 atcgatcgtc gccatacacg cacctcattg ttgtcggcgc tctctgtgtg gagcacctca
    10081 tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt
    10141 tcttattatc agagtgccac taatccgctt ctgaacggaa ttttatgctg gataaaaagg
    10201 gcgttcagca ggagatacta aagacgccat attgccgcag agtcagggag atgtgagtca
    10261 gctcaccata aaaaagccgt atgttgaata atattttcaa ctgagttatc aagatgtaat
    10321 tagattatta ttcttttact gtatctaccg ttatcggagt ggctatgcga gttgtcatac
    10381 tgggaagtgg tgtggtaggc gttgccagcg cctggtactt aaatcaggca ggacatgagg
    10441 tcaccgtcat tgatcgggag ccgggggccg ccctggaaac cagtgccgcg aatgccgggc
    10501 aaatctcccc cggatatgct gcaccgtggg cagcaccagg tgtgccttta aaagcgatta
    10561 aatggatgtt ccagcgccat gcgccgctgg cggttcgtct cgacggtacg cagtttcagt
    10621 tgaaatggat gtggcaaatg ttacgtaact gcgacaccag ccactacatg gaaaacaaag
    10681 ggcggatggt gcgtctggcg gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa
    10741 ccaatattca gtatgaaggg cgtcagggtg ggacgctgca actgttccgt accgaacaac
    10801 agtatgaaaa tgcgacccgc gatatcgccg tgctggaaga tgccggcgta ccgtatcagc
    10861 tgctggaatc cagccgcctg gcggaagtgg atcccgcgct ggcagaagta gcgcacaaac
    10921 tgacgggcgg cctgcagtta cccaatgatg aaaccggaga ctgccagctg tttacccaga
    10981 atctggcgcg gatggcagag caggcggggg ttaaattccg ctttaatacg cccgttgacc
    11041 aactgctttg cgacggcgag caaatctacg gcgtgaagtt tggcgatgaa gtgattaagg
    11101 ccgatgcgta tgtgatggcg tttggttctt actcgacggc gatgctcaaa ggcattgttg
    11161 atattccggt gtatccgctg aaaggctact cgctgactat tccaattgcg caggaagatg
    11221 gtgcgccagt atccaccatt cttgatgaaa cctacaaaat cgccattacc cgtttcgata
    11281 accgcattcg tgttggcgga atggcggaaa ttgttggttt taacaccgag ctgttgcaac
    11341 cgcgtcgtga aacgctggaa atggtggttc gcgatctcta tccacgcggc ggtcatgtcg
    11401 agcaggcgac tttctggacg ggtttgcgcc cgatgacgcc agacggtacg ccggttgtcg
    11461 ggagtacacg ctttaaaaat ctgtggctga ataccggtca cggcacgctc ggctggacga
    11521 tggcttgcgg ttccggtcag ttgttaagcg atctgctctc tggtcgcacg ccagcgatcc
    11581 catatgagga tctaagcgta gcgcgctaca gccgtggatt tacgccatca cgtccgggcc
    11641 atttacatgg cgcacacagc taaggaaacg agatgacccg tccgatacag gccagcctcg
    11701 atctgcaggc attaaaacag aatctgtcca ttgtccgcca ggccgcgccg cacgcgcgcg
    11761 tctggtcggt ggtaaaagcg aacgcttacg ggcatggtat tgagcgtatc tggagcgcac
    11821 tcggggccac cgatggcttt gcattactta acctggaaga ggcaataacg ttacgtgagc
    11881 gcggctggaa ggggccgatc ctgatgctgg aaggattttt ccatgctcag gatctggaga
    11941 tttatgacca gcaccgcctg accacctgcg tacacagcaa ctggcagctc aaagcactgc
    12001 aaaatgcgcg gctaaaagca ccgttggata tttatcttaa agtgaacagt gggatgaatc
    12061 ggttaggctt ccagtccgat cgcgtgctta ccgtctggca gcagttgcgg gcaatggcga
    12121 atgttggcga aatgaccctg atgtcgcatt ttgctgaagc ggaacatcct gatggaattt
    12181 ccggcgcgat ggcgcgtatt gagcaggcgg cggaggggct ggagtgtcgg cgttcgttgt
    12241 ccaattcggc ggcgactctg tggcacccgg aagcgcattt tgactgggtt cggcctggca
    12301 ttattttgta tggcgcttcg ccgtccggtc agtggcgtga tatcgccaat accggattac
    12361 gtccggtgat gacgctaagc agtgagatta ttggtgtcca gacgctaaaa gcgggcgagc
    12421 gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg
    12481 caggatacgc cgacggttat ccgcgccatg cgcctaccgg tactcctgtt ttagtggacg
    12541 gcgtgcgcac catgacggtg gggaccgtct cgatggatat gctggcggtt gatttaacgc
    12601 cttgcccgca ggcggggatt ggtacgccgg ttgagctgtg gggcaaggag atcaaaattg
    12661 atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt gatgtgcgcg ctggcgttac
    12721 gcgtcccggt tgtgacagtg taacttgttg taagccggat cggaggcagc gtcttctggt
    12781 tgcgaaaaaa tcatccatcc ggctggtcag caactgtagt tgttaatgtg acagagccat
    12841 tgcccatgat agtgtccatt aaaagaatgg acactatttc cccggaacct gaactcgccg
    12901 cacaggcgtt ctacataaaa cgcttacgct tcattgttga ctcggagtcg acttcagata
    12961 aatcacgctt cacccttgat gcagattcgg gttcatgttc agattgttga ttaacaggct
    13021 gcgagtcctg ttgtctttcc agactcaaca ggcccaactg ttgatccagg agctccaggt
    13081 cctcctgtac cttcacgcgc tccaggagtt ccgggtcatc caggaactcc agatccataa
    13141 ggtcctccag ctcctcgagt ccttccaggt caacctagcc ttccttgtgc aaaaagtcca
    13201 aaaggtcctt caggccctgc tgttttttca ggccctcgac ggctttgagc agcgtcggta
    13261 aattgtcttt gtcccacggg ttaccattac agtcaaacac cttcaggctc tggggctgcg
    13321 gcacctgtac cgttttaaac ttgttttctc cacagtagta aattattagt ttgggcggca
    13381 gccgtggtga agagatcaaa tcgttactag aacagtcaaa tactttcagg cgagtcaagt
    13441 tctccagcat ctcatcaggt attgagttaa tattgttgcc ggcacaggat agtttataca
    13501 ggtactgggg catcgacggc ggtaaaaatt gcaagaagtt ttcagaacag ttaatcactc
    13561 tcaggttagt cgacagctca ggcagccaga taatactgtt ttcatagcac ttcagtacca
    13621 tcagttttga gggctgcacc ttcggtaaca agatcaagac gttatctgta cagtcaagca
    13681 ccagcagcca ctttggcatc accttcggta acgcggtcaa cacgttttta ctgcagtaaa
    13741 attcaatcag atgtggaggc aaattctcag ggagtgaagt caatcccagg ttgctaagat
    13801 tgagcatact ccgtcttgta atgagacatt ccttcattct gcgcaatgct tctgcccggt
    13861 tttcaatccg gcttgctggc aggttttctt ccaggatatt ttccttgatt tcttccagac
    13921 tttctttcag gtcttcttgg ggaatttctg actgtttgtt ttgctggttt tcttccagga
    13981 tttcgtacag gtttttttgc aggttttctt gcaaaccatc ctgctgtata gcccattcgc
    14041 accatttggc gaccatcatg aactcgttat gttgctgatt cagacgagtc cccaacgtga
    14101 tgatggtatt gttgtccacc tgtatgttca ctttgcccgc gatactgagg tcaatgatct
    14161 gccagggggc atcaccccag ctgaattttt gcgggctgag atacttcatg ttctgcaaaa
    14221 tttgattctt gagaagagcc gccctggcgg gcgtcgtacg cctgggttaa cggaagatgg
    14281 gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta gccataatgc tacgtttcct
    14341 tgaaataaat atcctctggc agcgctggag acgtgaatgt ggtcaccgca aaggagccac
    14401 aggacgtcat ttgcgctgat atcactactc atcgttgact ttttccctgg tcagaaatgc
    14461 cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag ctctgacgta ttaaagaaaa
    14521 tacgagagtg aacgacccgg aaataatcgt tattaagcaa aggaataact ataagaaatc
    14581 gctcatgcat ccggtgctac aggcggatga aacgcctatt gtgtgggcta tgtcagtgcg
    14641 gcaggttgcg tgggggaata tgttcaggcg atgtcaggca ggagcccctg atggttgacc
    14701 ggtaatgcgg ggctggtttt tattacctgt tacagtcgga aaaagccctt acgctgactt
    14761 tcttgagccg gtgttttgag ttcattagcc agagcctgca atggtttgtc atccataatt
    14821 taaccttctt ctgatgttgg gatgagcata tcaaaatcag gaaatgacaa gtttattaca
    14881 ggctcaggct gcgttcagta aagcatcgca tccggcgata gatgctgcat taaatttaag
    14941 attcagcttc ctcttcagcc acccgaacgc caatcttcaa cacttcatta tcttctttct
    15001 cggccaccgt ccagatcatc ccggcaaact ctacctggtc accgacaacc ggtgccgcgc
    15061 ctagcaactg ctggacaatt tcacccagcg tttgctgttt atcacgatat tctcgcccgt
    15121 cttccaggcc atatatcagc gcaacatcag catatttggc gctggcttcg agaatgaagt
    15181 caccaaagaa gcgttgatcc agcgcgaccg gtggcgactg gctgaacagt ttaccgagcg
    15241 ccgggagatc gcgttcccga ccaattacac acaatacatc gccttcgcgc agtcgggtgc
    15301 tgccggtagg atgaagcaac tggttatcac gaaacagggc tgcaatacgc gtctcttttg
    15361 gcatatgcaa atcgcgcagt gccgcaccta cacaccattt atcagcactc aattgataaa
    15421 caaactgctc ccacggattt tccggatgaa tatccaggcc aacgcgtgac accggacgtc
    15481 cgacgggcgg aaccaccact ttggcttttt tcgccgccca cgagagtgat gttccctgca
    15541 agagcagtga aaccagaacc acaaagaagg cgacattaaa gaacagtcgc gcattctcca
    15601 gccccgccat catcgggaac acggctagga tgattggcac cgcgccgcgt aatcccaccc
    15661 agctgataaa cacgcgctca cgcagattga aaccgcggaa agggagcaat ccagcaaata
    15721 ccgaaagggg acgggcgaag aatatcatcc atgccgacaa aatgagcgcc ggaatggcaa
    15781 taggcagcag atcgcttggg ttaaccaata gccccagcac caggaacatg gcgatttgcg
    15841 ccagccaggc gaggccgtcg aaattttgca ggatgccgta gcggttgcga atcggccgat
    15901 tacccagcag aaaaccgcac agataaacag ccagaatacc gctgccttcc agcgccgtgg
    15961 ttaaagcaaa aattaaaata ccgccgctta atgccagcaa tggatataat ccggcaggca
    16021 gggcgatgcg attaatcatt tgcagcagta aataaccacc gccaagaccg atgacaatcc
    16081 cgaggccaaa ttgctgcaga atatcgacca cgaacatcca actgacactg ctttcatgct
    16141 gctggatcat tgcaatcagg gtaatcgtca gaaagaccgc cattggatca ttactgccgg
    16201 attcaatttc cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc agcagagaaa
    16261 agaccgctgc agcatcggtg gagccgacga tagcgccgat taataagcct tcaatcaaat
    16321 caagattaaa cagccacgcc gccatcatgc cggttaaacc agaggtgata agtacgccca
    16381 gcgtcgccag cgacagcgcc ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca
    16441 tcccgccgtc gagcaaaata atcgccagcg ccaggttact taccatgtag gcgaaggggt
    16501 aattatcaaa cgggataccg ccgacgccat cgactcccgc cagcatgccg atcgccagaa
    16561 aaataaccag aataggaatg ccaagacggg aagaaaatga actaagtaat atactgctgg
    16621 tgacaaggat ggatcctaag atgaaaaggc taattattgt tgtggcatcc aacggtcggt
    16681 tactcctgat tacgctgtct cttatataaa ccctaccata ttagcggcag agacagcgtt
    16741 ttacttagcc ctgaagcgtg atttttttac attttaagaa caggatgacc gctaatcgtc
    16801 aactgagcgc cttcccgggt gttattcaga atggcatgtg cgcccaacgg cagcgtaacc
    16861 gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc
    16921 aaaaacgcgt acactgactc caggttgtaa cctgcgtcat aatcattggg cgtgctgccg
    16981 ctaaagctac cgagaataat cgccttctga cgtggcaaaa ttcccgcatg atagagctgc
    17041 aacagcatac gttcgacgcg gaaagggtgc acgttaatat cttccagcac cagaataccg
    17101 ttctcaattt ttggcatcca cggtgtacca atcagtgaaa tcagcatcgc aagattgcct
    17161 ccccacaacg tgccttcggt ctgacatgtc gggccttccc cttgccattc aatggtgaag
    17221 gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag ttcatccgca
    17281 ccaaagtttg ctaccagcat ggggccgcta aaggtgatga cattgccttg cgccagaaga
    17341 ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga gtaacgggtc atgttgttgg
    17401 cgagccacca gtgcctgcca gtcaatatct gccagtaaac gactggcacc gtaaccgccg
    17461 cgtacagcca gcacgatggt gttgggggta gtcagtctgg caagggaatt gagatcttcc
    17521 agacgctccg tttccgtacc cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta
    17581 ttgacttgat gccccgcgtc ggtaaggcgc tggataccac gcagcgcggc gtgctgttta
    17641 atgctgtaac ccgatggggc aattaagtga aacagagaca tggcaattcc ttgctgacaa
    17701 cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc
    17761 aggctataag gatagaagaa gtgaaattga gatggtttgc ctttttgatt gtgttattag
    17821 cgggttgttc atcaaagcat gactatacga acccgccgtg gaacgcgaaa gttccggtgc
    17881 aacgtgcgat gcagtggatg ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc
    17941 cacaattgat cacggcgatt atcgctatcg aatcgggtgg taatccgaac gcggtgagta
    18001 aatcgaatgc cattggtttg atgcagttaa aagcttcaac ctccggacgt gatgtttatc
    18061 gccgtatggg ctggagtggt gagccgacga ccagcgagct gaaaaatcca gagcgtaata
    18121 tttcaatggg ggcggcttac ctgaatattc tggaaaccgg cccgctggca ggcattgaag
    18181 atccgaaggt actgcaatat gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc
    18241 tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg
    18301 acgagttcct cgatcacgta gcgcgaaatc atcctgcgcc gcaggctccg cgctatatct
    18361 acaaacttga gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc
    18421 ccgggcttct cgctcgagag agaaaataat ccgctgtaat tgccgctcca ccgtcgggct
    18481 gacgttaaga aaacggaagc tcagacgggg agtggtgatg gtttcattct tgccatcaat
    18541 cactttgcgc tcgctggtgg agattaactg ggcgtcaaag tgaaaaacac cccattgccc
    18601 catgttgact tcaatttgag cgaagcgcat accttcttgt aattcggcag gctttgctgt
    18661 ttccagtaat gcgcccatgc cgcctaacga caaatcatac aagcggaaac gtaacgtact
    18721 gttatccgcc agtttggtct ggcaaaaata aggcggatgg agtggggcgg agatgcggaa
    18781 atatcggcgt cgttgtacaa accataaggt gggaggcggt acggtaataa atgccggaag
    18841 ctgcaagtat tcactctgct gtagttgttc aacagtaaac tcgactttcg caccctgagt
    18901 ttcggcggta atggtaatgt gctgtgcctt tagcacggcg atgttgtctt cggcttgact
    18961 gccgaaatcc agcaccagtt tatccggggt tattgccagt attttgctga tcagctgccc
    19021 gccattccaa ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta acacgcccag
    19081 gacggctaac ggattttgtt tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc
    19141 ctgactgaca aactgtcttt ggtttatcgg tcatagttaa caaaacttaa tacaaatgcg
    19201 tgaatatttt ttacatgttg ttcttaaatc agccgacaaa cgcccagcat cgatcccgcc
    19261 cttgcctata cttagagcgt tgacgtaagc atttcttgcg accgattcat cgaaacgagg
    19321 gcctgaacat gggaattatt gcctggatta tttttggcct gatagccggc attatcggca
    19381 agctaatcat gccggggcgt gatggtggtg gatttttcct gacctgtatt ctcgggatag
    19441 tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg cattgacggc tccatcagcg
    19501 gttttaattt gcacagcttc ctggtggcgg tggtgggcgc tatcctggtg ctgggcgtat
    19561 tccgcctcct gcgaagagaa taagattttc ataaggcgga tagcgataca gatgccgcta
    19621 tccgctttca catcagaacg tatactcgac acctgcccag aaattgcgac cttcttcaat
    19681 aaacccttca ctgtaggcat attgggtatc aaacaggtta ttgaccgacg cattgacgct
    19741 gaagccatga cctaaggtgt aatcggctcg aatgtgggtc accgcaaaac cggcggcttt
    19801 ttgtgaaccg tcactgttgc tgtagctgga ggaacgcgcc tcttccgaca gcgttacgct
    19861 taacggctcc catggtttga gtgtcatcca tgcggtcatt gtctgcgttg gaagatcggt
    19921 tatcttgccg atgtctttgc gtttggcgtc agcgtggatc agagcgtagc tcaatcctac
    19981 atccagtata ttgctgattt tccccttaat accggcatcc agaccgctgt aatccaccgt
    20041 gccgctgttc tgattttgaa tggtatcggc attgatattg tgcgagagga tggcatcact
    20101 cacccggtta tagtaaacgc tgacctcaaa tccccagtcg cgcgtgaagg caccattcca
    20161 ggttaaatcc accccgcgag cgcgttccgg cttgagctgc gggttaacaa tcgctatctg
    20221 gttatacgca ggtttggatg tggtatagcg ttctttcagc gtcggaaagc gtgtgcggtc
    20281 atagtacgaa agcgccagag tgtcttcatt ggcaaagtgg tatttcccca tcacctgcca
    20341 gttaaaagct gactgattgt tgtcgtcata atgggtgatg ctgccatctt tctcgtgttt
    20401 tttcgcttct acgctatcgc gccagtcata gctgattccc gccacgacat cgacattatc
    20461 agcagcagcc cattgatatt cactggcgag cgaccaggta cgatcttcat agcgatcgta
    20521 agcggcgtgc ggcgcacctt tttcccggtg tacgtcatct ttccagttaa cggcaaacga
    20581 cagcagatcg ttttcacgca catcggctgc cagttgtaat ccggcaccgt cgctgtaatc
    20641 ggaataatgg ctgtagctgc cttttttatt tttcaaatca gccagcgagt tgtacatcat
    20701 cagcgtattt tcaaaggtgt cgcgatacag ccgacttttc agggtaaaac ggtcgtttag
    20761 ttgggtcgct ccctgataat aaaaactttc tttgtcatac tctggccact gccagtagcg
    20821 tgatttttga ccacaatttc cgctgtatgg cgggttatct ttttcaccat cctgcttaat
    20881 gtaagtcagc gtgtattcat cgttttcgcg cggtgtaaaa ccgagcttca caatgccgcg
    20941 tttatcatca gccgatgaat taatcatctt gccgtgtttg cctgcaatat cattatttac
    21001 accatgcggc aggccgagaa aatcctgctt tagctggcta ccgctgactt gcaaataccc
    21061 aagatcgctg ctggcggcaa atgaagcatg catatcgtag gcattgtcct gactacggct
    21121 ccatccctgg cgatatccca gacttgcttc ttcagggtgg tgatattaat ggctccgccc
    21181 atctgattag gcccatgaag cagcgacgaa taccctttgg aaacttcgac tgcccccaga
    21241 ttgttggtca gaatccgcgc cagatcgagg ttgccgtcat aggggacata aatgggcaca
    21301 ccgtcgaaat agaccggcac ctgccgacta tcaaagccac gaacttttac ctgttcttcg
    21361 ttgcggctgc ctgacttttg cagcaccacg ccggggacga cacttaatgc ctgagcgaca
    21421 ttctgtttat ccaaggcctt catggtgggt tgatcgagaa cggtggtcgt cgtcgatgat
    21481 accggactgg accatacggt cagcgtgtcg ctctcctgag ccgctacggc ctgctgggta
    21541 aaggcaagtg ataatgctgt gcagagataa cgttttttga gtctcataga ataaattccg
    21601 tttaacaaag aattaaatag aggctgtggg ttgcagactg actttaatgt cagctggtgc
    21661 cgcgtaataa ggcgcggagg taataaaaag gcgaatgccg cagtcgaggt aatttttgag
    21721 tgtcgtcaga ttaattccgc cggtcagagc cagcgtgcag tgaggagcca gcgacggcgc
    21781 tatttgcgca atttcagttg cctgttgcgg actaaatttg tcgagctgaa gtacgtctgg
    21841 ttgtgcgcgt aacgcggcga tagcctcttt cggcgtgtcg gcttcaacaa caattttctt
    21901 ctcgggtgcg tggcgacgta actgagcgat tgcgcctgac cagccctgat tgtcatgaag
    21961 aaaatggcgg tggttggcaa acaataatat ggtttccgca catccggcac gatgaattag
    22021 tcctccggca gccagaattg cctgcgaagc cagtaagcgg gtgcccggaa ttgcttttcg
    22081 ggtgcaggcg atattaccgt caggataacg ttcacgaagc aatgccagca tttgagcgag
    22141 ataatcagaa acaccgcaac tccactccag cacattctgg actgccttcc atccctgatg
    22201 aagtgctgcg gcattaccct gcgcacggat tagccgttga cccgcgttcg cttgtgaacc
    22261 gtcgctgacc gcgtcatcaa tagttaatcc cagcgtagtt aacatcttac acgcgacaga
    22321 aacgccgctg acgcagccgc cctgacggtg aaaaaacgct atatagccag gctggtgtcc
    22381 aatatttaat gctcgcgtgg tcaggtcacc tccctggata tcttccagca gtaacgcatc
    22441 gatttgtgcc tgagaaagga aaatcatcac agtgcctcct gcggtacgct atcccaccag
    22501 atgagcgccc agtcgcggct ggcaggggcg agggcacgag catcttgttg ttgataccag
    22561 tgatataacc gctcacgctc atcgtcgtta agcgcgccaa gactccagct gacgttctgc
    22621 tcaaaacgtt cccaggtgct gttatcctgt tggcaatttt gtccacggat aaaatcaacg
    22681 tgggcataaa ttcccatctg atacaggacg ttgagcgcaa agatatagtt gggtaattcg
    22741 atcacttccc gaccagccgc ccgctgaatg gctggagaga cgaacgaggt gctgaccaga
    22801 tgagttgtat agacgcgaag ccgtgcctga ttattcagtt tgctcattgc atggcgcata
    22861 tcagccacca gagtggagcg ggaagccacg gcaatatcgc agcgtggcag gtcactccag
    22921 tcctcttccc aggcgcgctg gatccagtgg acattgtcag ccttcagtgc ggcggcgcgg
    22981 cgggcggcaa cgttcagcat cccctgactg taatcaacgc cataaaccgt agtgagtttg
    23041 tcagccagcg ccagcgatac ggtgcctggt ccgcagccca tatcgaataa ggtctgagcg
    23101 ccttgcaggt caattttagc gatcagttgt tgcaggtagc tgtccgttgg cgaggcgcaa
    23161 ttttccgcca ttttctccgc ccgttgatcc cagtgatcgg gcgttttttc tgtgcggtga
    23221 gccagtttga gttgctgcag ataaaggtct gcaaaatcaa tatcatcaat gagcatgatt
    23281 gttccttagc ggctaattgc agagaggtga tgatgaattt ggtcggcaga aacccgatac
    23341 agggccgcca gtttgttcgt tgttaactgt tcggtcggta agccttgtgt tactcgtcca
    23401 tctggctcta cttggataat gctgtcggca atggcgttgg catggagcgg atggtgagtc
    23461 gacattaaca ttgtcatgcc gttatttttg agctgcgcca gcgtgtccag aagttggatc
    23521 tgatgaccga aatcgaggct ggaggcgggc tcatccagta acaacaagcg cggttgttgg
    23581 acgagtgcac gggcgattaa agccagctga cgttcaccgc cgctaagcgt attccagcgt
    23641 cgtgtcgcca gatgcaaaat gcccagcttt tccagttgct ccgtggcttt caggcgttct
    23701 tgcttaccgg gaacggaaaa agcgcctatg gtgggggcca ggcccatcaa caccatatcc
    23761 agcacactga aagcaaaaat accgtcgtgc gcctgcggca cccaggcaat agcctgtgcg
    23821 cgctggcgat ggctgagtgt ctgcacagag aggctatcca gtaggatctc gccttttaat
    23881 aacggtataa cgccaagaat ggagcgcatg agtgtggttt tgccgcaacc attcgcgcca
    23941 agcagacaac aaattgttcc ttgcgacagg gtgaaggaca cgtcgcgtag cactggatgg
    24001 cgggagtaac ccagagacgc atgacgaacg gcaagcaaag tcactggcga cctccgcgta
    24061 acaacaaaag caggaaaaac ggtgcgccaa caaaagcggt cagaatgcca agtgggattt
    24121 ctgttgttcc aatgctgcgc gccagcgtat cggtaagcag gagcagaata gcaccagtac
    24181 acattgccat aggcaacagc tgctgatggt tatgaccggt caacaagcgg gcaatgtgcg
    24241 gaacgaccag accgatccaa ccaattatgc ccgctattgc taccgtgctg gcggtgatta
    24301 gtgtggcgca gacaatcaat ccaaagcgca gacgcgtcac gttcagtccc agcgaacggg
    24361 cttcgtcgtc ggaaagcgtg agtaaattca tgcgccagcg gagaaaaaac aggggcaagg
    24421 agcctatgag aatgatcggt gcagcatagc agagatcgcg taaggtcacg gtggacagtc
    24481 cccccagcaa ccagaaggta attgacggta actgggtgta aggatcggcc agcgttttaa
    24541 ttagcgatat ccccgctccg cacaacgttc ctaacgcaat gcctaccagc accagggtta
    24601 agatcggatc gtggcgggtc acccgacgcg taatcaacca gacgcccgcg accaccatca
    24661 gcccgccgca aaatgcgtac agctggatgt aaacaatggg taatccccac aggattgccg
    24721 cgcaggcacc aagtccggca ccggcggaga cgcctaaaat atccggtgaa actaagggat
    24781 tacgcaacat tccctgatag gtggttccgg ctcctgccag cgctgcgccc agtaacagcg
    24841 cagcaagaat acgcggcacg cggatttgcc agaagacaat ttgttcctgc acagactcat
    24901 tttcttgacg aagaattagc gccagcagtt gctgcatatc gagatgataa gccccactta
    24961 cggcggcaaa agtgatactg atagctgcca gcagcaaaat acatccgttt acgattctca
    25021 ttagctcgcc accaattttt gatagtcggc atcactgagt gaacaatgcc agaacagttg
    25081 ggcgtaatgc tgcatgtcac ttttaaactg gcgattgatg gcgggatcca gccaggcgtg
    25141 aagtctgcgt aatcccagaa gacggttgat tcccggcggg gcatcgagcc agccaaaggg
    25201 caagccactt aaaaataaga tgcgttgttc cgcaacggct ttcacgccct gccagagagg
    25261 atcacgacga ataaaatctg cagtaacggc ctcctgaacc agaataatat ccggctgcca
    25321 gcgcaggaga ttttccatgg aaacctgtgt caggccgtgg cgatcggcta tttgcgcgac
    25381 gttgtgcaaa cctaataatt ccgccgcctc ggtatgcaac gaaccctgta agcccgtttc
    25441 cagccctcgg ggaccgcgcg cagcataaaa gcggaggtta gcggcgggtg aggtggcgaa
    25501 tgcctgagct tcaccaacga agtggctggc gagattggct tgttctgcgg cgcggtgctc
    25561 ttcgcctaac gttttgccaa gcgttgttaa ctgttctgct gattgcgcca gttttccgtt
    25621 aagcaataac caggggattt gtgtctgttc gctaacctgc cgtgcctggg agatcaaggt
    25681 ttcatccgta ttgccgcaat caacgaccaa atcgggatgt aacgccataa gcccttccag
    25741 cgatagtgtg ctggcgcgcc cggcgagtcg tcccagcctg ggtaactggc gaatgtgctc
    25801 tggcaatgga attaatgcct gacgggcaaa atcaaaagag gaaaaaccga ccattttttc
    25861 tggcgcgaca gccagcaaga gcaaatcggc gggcgcgcct gcgctgacga tgcgtgtaat
    25921 gttttgggct tcgggaagag attgcgaagc aaaacgatta actgtttgtg aaaaggacgg
    25981 tagagactgc aggagcaggg tagatgcaag tgcctgcgca aacgtccgtc tggttatggg
    26041 catggagaac atcctgaaaa gtaaaatcga tatatacttt catatatatc gataaaaatc
    26101 atgttcaata tcaaagttta gctattgaaa acatgaagat gtgacgactt ccgcaagcct
    26161 gttgaccctc tctatcatta aaaatgtatt tatcaggtgg ctaatattat ttaaatcatt
    26221 taaggctaaa tcatttaaat atgaatgaca ttattgtttt ttatcgtcca cggttacgcg
    26281 acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag aaaattatca
    26341 gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag aatgctgggg
    26401 gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag taagcagcgt
    26461 aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat taatgtcatt
    26521 aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc gtttattcgg
    26581 acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct ttattttgat
    26641 aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga gttgcagtta
    26701 ttaagcacag ggtgtagcaa tgataacatt ctc
//
LOCUS       DAJTNW010000059        26077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNW010000059 DAJTNW010000000
VERSION     DAJTNW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO179_002504"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO179_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5532526.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO179_002505"
     CDS             complement(2138..2584)
                     /locus_tag="OO179_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5532527.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO179_002506"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO179_002506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5532528.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO179_002507"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO179_002507"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5532529.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO179_002508"
     CDS             complement(5347..6009)
                     /locus_tag="OO179_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5532530.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO179_002509"
     CDS             6182..6595
                     /locus_tag="OO179_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5532531.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO179_002510"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO179_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5532532.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO179_002511"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO179_002511"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5532533.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO179_002512"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO179_002512"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5532534.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO179_002513"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO179_002513"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5532535.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO179_002514"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO179_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5532536.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO179_002515"
     tRNA            complement(9861..9948)
                     /locus_tag="OO179_002515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO179_002516"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO179_002516"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5532537.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO179_002517"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO179_002517"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5532538.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO179_002518"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO179_002518"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5532539.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO179_002519"
     CDS             14005..14592
                     /locus_tag="OO179_002519"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5532540.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO179_002520"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO179_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5532541.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO179_002521"
     CDS             14984..15841
                     /locus_tag="OO179_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5532542.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO179_002522"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO179_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5532543.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO179_002523"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO179_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5532544.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO179_002524"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO179_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5532545.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO179_002525"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO179_002525"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5532546.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO179_002526"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO179_002526"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5532547.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO179_002527"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO179_002527"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5532548.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OO179_002528"
     CDS             complement(22898..24037)
                     /locus_tag="OO179_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5532549.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="OO179_002529"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="OO179_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="OO179_002530"
     CDS             complement(25322..26017)
                     /locus_tag="OO179_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5532550.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6306 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcggat
//
LOCUS       DAJTNW010000060        25414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNW010000060 DAJTNW010000000
VERSION     DAJTNW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="OO179_002531"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="OO179_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OO179_002532"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OO179_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5532551.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OO179_002533"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OO179_002533"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5532552.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OO179_002534"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OO179_002534"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5532553.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="OO179_002535"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="OO179_002535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5532554.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7840..7915
                     /locus_tag="OO179_002536"
     tRNA            7840..7915
                     /locus_tag="OO179_002536"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7873..7875,aa:Gly,seq:gcc)
     gene            7951..8026
                     /locus_tag="OO179_002537"
     tRNA            7951..8026
                     /locus_tag="OO179_002537"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7984..7986,aa:Gly,seq:gcc)
     gene            8259..8312
                     /locus_tag="OO179_002538"
     CDS             8259..8312
                     /locus_tag="OO179_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532555.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8295..9434)
                     /gene="queG"
                     /locus_tag="OO179_002539"
     CDS             complement(8295..9434)
                     /gene="queG"
                     /locus_tag="OO179_002539"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5532556.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9433..10980
                     /gene="nnr"
                     /locus_tag="OO179_002540"
     CDS             9433..10980
                     /gene="nnr"
                     /locus_tag="OO179_002540"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5532557.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            10952..11413
                     /gene="tsaE"
                     /locus_tag="OO179_002541"
     CDS             10952..11413
                     /gene="tsaE"
                     /locus_tag="OO179_002541"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5532558.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11432..12769
                     /gene="amiB"
                     /locus_tag="OO179_002542"
     CDS             11432..12769
                     /gene="amiB"
                     /locus_tag="OO179_002542"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5532559.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12779..14626
                     /gene="mutL"
                     /locus_tag="OO179_002543"
     CDS             12779..14626
                     /gene="mutL"
                     /locus_tag="OO179_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5532560.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14619..15569
                     /gene="miaA"
                     /locus_tag="OO179_002544"
     CDS             14619..15569
                     /gene="miaA"
                     /locus_tag="OO179_002544"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5532561.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15655..15963
                     /gene="hfq"
                     /locus_tag="OO179_002545"
     CDS             15655..15963
                     /gene="hfq"
                     /locus_tag="OO179_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5532562.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16039..17319
                     /gene="hflX"
                     /locus_tag="OO179_002546"
     CDS             16039..17319
                     /gene="hflX"
                     /locus_tag="OO179_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5532563.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRACTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17405..18664
                     /gene="hflK"
                     /locus_tag="OO179_002547"
     CDS             17405..18664
                     /gene="hflK"
                     /locus_tag="OO179_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5532564.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18667..19671
                     /gene="hflC"
                     /locus_tag="OO179_002548"
     CDS             18667..19671
                     /gene="hflC"
                     /locus_tag="OO179_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5532565.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19753..19950
                     /locus_tag="OO179_002549"
     CDS             19753..19950
                     /locus_tag="OO179_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5532566.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20054..21352
                     /gene="purA"
                     /locus_tag="OO179_002550"
     CDS             20054..21352
                     /gene="purA"
                     /locus_tag="OO179_002550"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5532567.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21557..21982
                     /gene="nsrR"
                     /locus_tag="OO179_002551"
     CDS             21557..21982
                     /gene="nsrR"
                     /locus_tag="OO179_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5532568.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22021..24462
                     /gene="rnr"
                     /locus_tag="OO179_002552"
     CDS             22021..24462
                     /gene="rnr"
                     /locus_tag="OO179_002552"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5532569.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24642..25373
                     /gene="rlmB"
                     /locus_tag="OO179_002553"
     CDS             24642..25373
                     /gene="rlmB"
                     /locus_tag="OO179_002553"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5532570.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5942 a         6456 c         7125 g         5891 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7861 gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccaaaatt
     7921 taaaagtgct gtaaggcaca gaccacccaa gcgggaatag ctcagttggt agagcacgac
     7981 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaatt cttctctcaa
     8041 taaaatatct acagcgacgc gatgcgttat tgctggtttt tgttgtctct gacaaactct
     8101 tgtaaacaga gttatccaca gcctcaggct gtaatcttaa tttcaaagaa acttcgcacg
     8161 gtgaatggta ttttttaacc tattgataaa taagttaaaa atcaagattc cgttttgtcg
     8221 agtcgatcac ttgacgattt tattcgtctt gaattgcaat gcgtttttat ttttattcac
     8281 aagctgtgga tgaatcaggc gtcacgcggt aacccttttt caatcacccg aaccagacgc
     8341 tgttttttcg gcaattgcac ttcgactatg cacgcatttc gtctctctat ttgctgcgca
     8401 atcgcccacg ctatgtgctc atcgagaagt gggtgctcac ctttacgact ttccagcgct
     8461 gtcaaaatcg tttcatccca aggggcattg cctaatgcca cggcgatatt acgcagccag
     8521 cgcaggtgac ctatacggcg aatcgccgat ccttccgtga cttttaaaaa cttctcttcg
     8581 ctccaggcaa ataactcaat gagttccggt gcgtgtagcg gcttacgcgg gctgaaatcg
     8641 tcttctgtag tgagttgcga atagcgattc cacgggcaga taagctggca gtcatcgcaa
     8701 ccgtaaatac ggtttcccat taacggtcgc aactcttccg ggagcgcccc ttcaagttcg
     8761 atggtgagat aagagataca gcggcgagca tcgacggtat atggctcgac gatggcaccg
     8821 gtcgggcaaa tcgtcataca ggccacgcat ttgccgcatc cttcctcgac tggttgatcc
     8881 acgggcagcg gaatatcgac cagcaattcg cctaaaaaga agaacgaacc ggcctcgcga
     8941 ttgaggataa gtgagtgctt acctgtccag ccgagcccag ctttttcagc taacgggcgc
     9001 tcgagaatag gcgcagaatc gacaaacggt ctaaaattca gcgaaacaca atgttgctga
     9061 atcatctcgc ccagcttttt gagtcggttg cgcagaagtt tgtgatagtc acggcccagc
     9121 gcataacggc taacatagcc gagtttgggg tttttcagcg tgctggcaaa tgcggcgtta
     9181 gcaggaaggt aattcatccg cacgctgatt acacgcagcg taccgggtaa taactcatgg
     9241 gggcgagcgc gcagcatacc gtgacgtgcc atccagtcca tttcgccgtg gtattgtttg
     9301 tccagccatg cttgcagttt gggctcggac tcgctgagat cggtatcggt aatacctacc
     9361 tgctgaaagc ccagttccag cccccactgt ttaatttttt gcgctaactg attgagatcg
     9421 aggggctctg acatgacgga ccatacaatg aagaaaaacc ccgtaagtat accacacacc
     9481 gtctggtacg ccgacgatat ccgccgcgga gaacgcgagg cggcagatgt gctggggctc
     9541 acactctatg agctgatgct tcgcgctggc gaggccgcat tccaggtgtg tcgttcggcg
     9601 tatcctgacg cccgccactg gctggtgctg tgcggtcatg gtaataacgg cggcgatggc
     9661 tacgtggtcg cgcgactggc caaagcggtc ggcattgagg tcacgttgtt ggcccaggag
     9721 agcgacaaac cgttgccgga agaggccgcg ctggcacgcg aagcatggtt aaacgcgggt
     9781 ggcgagatcc atgcttcgaa tattgtctgg cccgaatcgg tagatctgat tgttgatgcg
     9841 ctgctcggta ccggcttgca gcaagcgccc cgcgaatcca ttagccagtt aatcgaccac
     9901 gctaattccc atcctgcgcc gattgtggcg gttgatatcc cttccggcct gctggctgaa
     9961 accggcgcta cgccaggcgc agtgatcaac gccgatcaca ccatcacttt tattgcgctg
    10021 aaactaggct tgctcactgg aaaagcgcgg gatgttacag gacaactgca ttttgactca
    10081 ctggggctgg atagctggct ggcaggtcag gagacgaaaa ttcagcggtt ttcggcagaa
    10141 caactttctc actggctgaa accgcgtcgc ccgacttcgc ataaaggcga tcacgggcgg
    10201 ctggtgatta tcggtggcga tcacggcacg gcgggggcta ttcgtatgac gggggaagcg
    10261 gcgctacgtg ctggtgctgg tttagtccga gtactgaccc gcagtgagaa cattgcgccg
    10321 ctgctgactg cacgaccaga attgatggtg catgaactga ctatggactc tcttaccgaa
    10381 agcctggaat gggccgatgt ggtggtgatt ggtcccggtc tgggccagca agagtggggg
    10441 aaaaaagccc tgcaaaaagt tgagaatttt cgcaaaccga tgttgtggga tgccgatgca
    10501 ttgaacctgc tggcaatcaa tcccgataag cgtcacaatc gcgtgatcac gccgcatcct
    10561 agcgaggccg cacggttgtt aggctgttcc gtcgctgaaa ttgaaagtga ccgcttacat
    10621 tgcgccaaac gtctggtaca acgttatggc ggcgtagcgg tgctgaaagg tgccggaacc
    10681 gtggtcgccg cccatcctga cgctttaggc attattgatg ccggaaatgc aggcatggcg
    10741 agcggcggca tgggcgatgt gctctctggt attattggcg cattgcttgg gcaaaaactg
    10801 tcgccgtatg atgcagcctg tgcaggctgt gtcgcgcacg gtgcggcagc tgacgtactg
    10861 gcggcgcgtt ttggaacgcg cgggatgctg gcaaccgatc tcttttccac gctacagcgt
    10921 attgttaacc cggaagtgac tgataaaaac catgatgaat cgagtaattc cgctccctga
    10981 tgagcaggca acattagacc tgggcgagcg ggtagcgaaa gcctgcgatg gcgcaaccgt
    11041 aatctatctg tatggcgatt taggcgcagg taaaaccacc tttagccggg gctttttaca
    11101 ggctctgggt catcagggta atgtcaaaag ccccacttat acgctggtcg aaccctatac
    11161 gctcgacaac ttaatggtct atcactttga tttgtaccgc cttgccgatc ccgaggagct
    11221 ggagtttatg gggatccgcg attattttgc caacgatgcc atctgcctgg tggagtggcc
    11281 acaacaaggt acaggtgttc ttcctgaccc ggatgtcgaa atacacattg attatcaggc
    11341 acaaggccgt gaggcgcgcg tgagtgcggt ttcctctgcg ggtgaattgt tgctggcgcg
    11401 tttagccggt taacctttga aaggtggcgg gatgatgtat cgcatcagaa attggttggt
    11461 agcgacgctg ctgctgctgt gcacgccggt gggtgccgcg acgctctctg atattcaggt
    11521 ttctaacggt aatcaacagg cgcggataac gttgagtttt attggcgatc ctgattatgc
    11581 gtttagccat caaagcaaac gcaccgtggc gctcgatatc aaacaaacgg gcgtgattca
    11641 gggactgccg ttgttgttca gcggcaataa tctggtgaag gcgattcgct ctggaacgcc
    11701 taaagatgca caaacgctac ggctggtggt cgatcttacc gaaaacggta aaaccgaagc
    11761 ggtgaagcgg cagaatggca gcaattacac tgtcgtcttt acgattaacg ccgatgtgcc
    11821 gccaccgcct cctccgccgc ccgtggttgc gaaacgcgtt gaaacgcctg cggttgtcgc
    11881 accgcgcgtc agcgaaccgg cgcgcaatcc gtttaaaacg gaaagtaacc gcactacggg
    11941 tgttatcagc agtaatacgg taacgcgtcc ggcagcgcgc gcgacggcta acactggcga
    12001 taaaattatc atcgctattg atgccggaca cggcggtcag gaccctggcg ctatcggccc
    12061 cggtggtacg cgggagaaaa atgtcaccat cgccatcgcg cgtaaattgc gtactttgct
    12121 caatgacgat ccgatgttta aaggcgtttt aacccgtgac ggggattact ttatctcggt
    12181 gatggggcgc agtgatgtgg cacataagca aaacgccaat ttcctcgtgt cgattcacgc
    12241 tgatgccgca ccgaaccgta gtgcgactgg cgcttccgta tgggtgctct ctaaccgtcg
    12301 cgccaacagt gaaatggcca gctggctgga gcagtacgag aaactgtcgg agctgctggg
    12361 tggggcgggt gatgtgctgg cgaacagtca gtctgacccc tatttaagcc aggcggtgct
    12421 ggatttacag ttcggtcatt cccagcgggt agggtatgat gtagcgacca gtatgatcag
    12481 tcagttgcaa cgcattggcg aaatacataa acgtcgacca gaacacgcca gccttggcgt
    12541 tctgcgctcg ccggatatcc catcagtact ggtcgaaacc ggttttatca gcaacaacag
    12601 cgaagaacgt ttgctggcga gcgacgatta ccaacaacag ctggcagaag ccatttacaa
    12661 aggcctgcgc aattacttcc ttgcgcatcc gatgcaatct gcgccgcagg gtgcaacggc
    12721 acaaactgcc agtacggtga cgacgccaga tcgcacgctg ccaaactaag gacgattgat
    12781 gccaattcag gtcttaccgc cacaactggc gaaccagatt gccgcaggtg aggtggtcga
    12841 gcgacctgcg tcggtagtca aagaactagt ggaaaacagc ctcgatgcag gtgcgacgcg
    12901 tatcgatatt gatatcgaac gcggtggggc gaaacttatc cgcattcgtg ataacggctg
    12961 cggtatcaaa aaagatgagc tggcgctggc gctggctcgt catgccacca gtaaaatcgc
    13021 ctctctggac gatctcgaag ccattatcag cctgggcttt cgcggtgagg cgctggcgag
    13081 tatcagttcg gtttcccgcc tgacgctcac ttcacgcacc gcagaacagc aggaagcctg
    13141 gcaggcctat gccgaaggac gcgacatgga cgtgacggtt aaaccggcag cgcatccggt
    13201 gggaacgacg ctggaggtgc tggatctgtt ctacaacacc ccggcgcggc gcaaattcct
    13261 gcgcaccgag aaaaccgaat ttagccatat tgatgaaatc atccgccgca ttgcgctggc
    13321 gcgtttcgac gtcacgatca acctgtcgca taacggtaaa attgtgcgtc agtaccgcgc
    13381 agtgccggaa ggcgggcaaa aagaacggcg cttaggcgcg atttgcggca ccgcttttct
    13441 cgaacaagcg ctggcgattg aatggcaaca cggcgatctc accctgcgcg gctgggtggc
    13501 cgatccaaat cacactacgc ccgcactggc ggaaattcag tattgctacg tgaatggtcg
    13561 catgatgcgc gatcgcctga tcaatcatgc gatccgccag gcctgcgaag acaaactggg
    13621 ggccgatcag caaccggcat ttgtgttgta tctggagatc gacccgcatc aggtggacgt
    13681 caacgtacac cccgccaaac acgaagtgcg tttccatcag tcgcgtctgg tgcatgattt
    13741 tatctatcag ggcgtgctga gcgtgctaca acagcaactg gaaacgccgc taccgctgga
    13801 cgatgaacca caacccgcac cgcgtgccat cccggaaaac cgcgtggcgg cggggcgcaa
    13861 tcactttgca gaaccggcag ctcgtgagcc ggtagctccg cgctactctc ctgcgcccgc
    13921 ctcaggcagt cgtccggctg ccccctggcc gaatgcgcag ccaggctacc agaaacagca
    13981 aggtgaagtg tatcgccagc ttttgcaaac gcccgcgccg atgcaaaaac caaaagcgcc
    14041 ggaaccgcag gaacctgcac ttgcggcgaa cagtcagagt tttggtcggg tactgactat
    14101 cgtccattcc gactgtgcgt tgctggagcg cgatggcaac atttcacttt tatccttgcc
    14161 agtggcagaa cgttggctgc gtcaggcaca attgacgccg ggtgaagcgc ccgtttgcgc
    14221 ccagccgctg ctgattccgt tgcggctaaa agtttctggc gaagaaaaat cggcattaga
    14281 aaaagcgcag tctgccctag cggaattggg tattgatttc cagtcagatg cacagcatgt
    14341 gaccatcagg gccgtgcctt tacccttacg ccaacaaaat ttacaaatct tgattcctga
    14401 actgataggc tacctggcga agcagtccgt attcgaacct ggcaatattg cgcagtggat
    14461 tgcacgaaat ctgatgagcg aacatgcgca gtggtcaatg gcacaggcca taaccctgct
    14521 ggcggacgtg gaacggttat gtccgcaact tgtgaaaacg ccgccgggtg gtctgttaca
    14581 atctgttgat ttacatccgg cgataaaagc cctgaaagat gagtgatatc agtaaggcga
    14641 gcctgcctaa ggcgattttt ttgatggggc cgacggcctc cggtaaaacg gcgttagcca
    14701 ttgagctgcg taaaatttta ccagtagagt tgataagcgt tgattctgcc cttatttaca
    14761 aagggatggt tatcgggacg gcgaagccga acgctgaaga gttactcgcc gcgccgcacc
    14821 gattgctgga tattcgcgat ccgtcgcagg cttactcggc tgctgatttt cgccgcgatg
    14881 cgctggcgga aatggccgat atcaccgcgg cggggcggat cccactgtta gtgggcggta
    14941 cgatgttgta tttcaaggca ttgctggaag ggttgtcgcc gctaccgtcg gcagacccgg
    15001 aagtgcgggc cagaattgag caacaggcgg cagagcaagg ttgggagtca ttgcatcgtc
    15061 aacttcagga ggtagatccg gttgcggcag caaggattca tccaaatgat ccacaaaggc
    15121 tttcccgggc actggaagtt tttttcattt cgggtaaaac tttaacggaa ctgacgcaaa
    15181 cgtcaggaga cgctctaccg tatcaggtgc atcagttcgc catcgccccg gcgagccgtg
    15241 aactgctcca tcaacgcatt gagcagcgtt ttcatcagat gttggcttca ggttttgaag
    15301 cagaagtccg ggcgcttttt gcccgaggag atttgcatac ggacttgcct tccattcgtt
    15361 gcgtgggtta tcgccagatg tggtcttacc ttgaaggcga aatctcatac gatgaaatgg
    15421 tttatcgagg tgtttgcgcc acgagacagt tggcgaagcg gcagataacc tggctgcgtg
    15481 gttgggaagg ggttcactgg cttgacagtg aaaaaccaga acaggcgcgt gacgaagtat
    15541 tacaggttgt tggtgctatc gcaggctgaa tgtgtacaat tgagacgtat cgtgcgcaat
    15601 tttttcagaa tcgaaaggtt caaagtacaa ataagcatat aaggaaaaga gagaatggct
    15661 aaggggcaat ctttacaaga tccgttcctg aacgcactgc gtcgggaacg tgttccagtt
    15721 tctatttatt tggtgaatgg tattaagctg caagggcaaa tcgagtcttt tgatcagttc
    15781 gtgatcctgt tgaaaaacac ggtcagccag atggtttaca agcacgcgat ttctactgtt
    15841 gtcccatctc gcccggtttc tcatcacagt aacaacgccg gtggcggtac cagcagtaac
    15901 taccatcatg gtagcagcgc gcagaatact tccgcgcaac aggacagcga agaaaccgaa
    15961 taaggtttcg ggctgttttt ttacacgggg agccagcgat cctgcgttcc ccgctgatct
    16021 atttagaggg ttatacgctt gtttgaccgt tatgatgctg gtgagcaggc ggtactggta
    16081 cacatctatt ttacgcaaga caaagatatg gaagacctcc aggagtttga atctctggtc
    16141 tcttccgccg gtgtcgaagc attgcaggtg attaccggta gccgtaaagc gccgcaccca
    16201 aagtattttg taggtgaagg taaagcagtt gaaattgcgg aagctgtcaa agcgatgggt
    16261 gcttcggtcg ttctttttga ccatgccctg agcccggcgc aagagcgtaa cctggagcgt
    16321 ttgtgcgagt gtcgtgttat cgaccgcacc ggccttattt tagatatttt cgcccaacgt
    16381 gcgtgtaccc atgagggtaa gttgcaggtt gagctggcgc agctgcgcca tctggctacg
    16441 cgcctggtgc gtggctggac ccaccttgaa agacagaaag gcgggatagg tttgcgtggt
    16501 ccgggtgaaa cccagctcga aaccgaccgt cgtttgttgc gtaatcgcat cgtgcagata
    16561 cagtcgcgcc tggaaagagt tgaaaagcag cgtgagcagg ggcggcaatc gcgtatcaaa
    16621 gccgacgttc ctactgtttc gctggtggga tataccaacg ccggtaaatc tacccttttc
    16681 aatcgcatca ccgaagcgcg ggtctacgcg gcagaccagt tgtttgccac cctcgacccg
    16741 acgttgcggc gtattgacgt tgcagatgtc ggtgaaaccg tacttgcaga taccgtaggg
    16801 tttattcgcc acctgccgca cgatctggtg gcggcattta aagccacgtt acaagagacg
    16861 cggcaagcca cattactgct gcacgtcatt gatgcggcgg atgtgcgtgt acaagaaaac
    16921 atcgaagcgg tgaatacggt tcttgaagag atcgacgctc acgagatccc aaccctgctg
    16981 gtgatgaaca agatcgatat gctggaagat ttcgaaccgc gtattgatcg ggacgaagag
    17041 aacaaaccga tccgtgtctg gctttccgca cagaccggag cggggatacc acagcttttt
    17101 caggctttga cggagcggct ttccggcgag gtggcgcagc atacattgcg tctgccaccg
    17161 caggaagggc gtctgaggag tcgtttttat cagcttcagg caatagaaaa agagtggatg
    17221 gaggaggacg gcagcgtaag tctgcaagtt cgtatgccga tcgttgactg gcgtcgcctc
    17281 tgtaaacaag aaccggcgtt gatcgattac ctgatctaac ggcgtagcgt ctgaagcgtg
    17341 gagtcatatc ctctggcgtc gaaagacaac agggatcacc gcataacaaa tatggagcac
    17401 aaacatggcg tggaatcagc ccggtaataa cggacaagac cgcgacccgt ggggaagcag
    17461 caaacctggc ggcaactctg agggaaatgg aaacaaaggc ggtcgcgatc aagggccacc
    17521 tgatttagat gatatcttcc gcaaactgag caaaaagctc ggtggtctgg gcggcggtaa
    17581 aggcaccgga tctggcggtg gcagttcatc gcaaggcccg cgcccgcagc ttggcggtcg
    17641 tgtcgttacc atcgcagcgg cagcgattgt cattatctgg gcggccagtg gtttctatac
    17701 cattaaagaa gccgaacgcg gcgtggtaac acgctttggt aaattcagcc atctggttga
    17761 gccgggtctg aactggaaac cgacgtttat cgacgaagtc aaaccggtga acgtggaagc
    17821 cgtgcgtgaa ctggccgctt ctggtgtgat gctgacgtcg gacgagaacg tagtgcgcgt
    17881 tgagatgaac gtgcagtacc gcgtcaccaa tccggaaaaa tatctgtata gcgtgaccag
    17941 cccggatgac agcctgcgtc aggctaccga cagcgccctg cgtggagtta tcggtaaata
    18001 caccatggac cgcattctga cggaaggtcg taccgtgatt cgtagcgata ctcagcgcga
    18061 actggaagag acgattcgtc cgtatgacat gggtatcacg ctgctggacg tcaacttcca
    18121 ggctgctcgt ccgccggaag aagtaaaagc ggcgtttgac gatgcgattg ccgcgcgtga
    18181 aaacgaacag caatacattc gtgaagcaga agcgtatacc aacgaagttc agccgcgtgc
    18241 gaacggtcag gcgcaacgta tcctcgaaga ggcgcgtgcg tacaaggccc agaccatcct
    18301 ggaagctcag ggtgaagtgg cgcgctttgc taaacttctg ccggaatata aagccgcgcc
    18361 ggaaattact cgcgagcgtc tgtatatcga gacgatggaa aaagtgttgg gtaacacccg
    18421 caaagtgctg gttaacgata aaggtggcaa cctgatggtt ctgccgttag accagatgct
    18481 gaaaggtggt aacgcccctg cggcgaagag cgataacggt gccagcaatc tgctgcgtct
    18541 gccgccagcc tcttcctcca caaccagtgg agcaagcaac acgtcgtcca ccagtcaggg
    18601 cgatattatg gaccaacgcc gcgccaacgc gcagcgtaac gactaccagc gtcaggggga
    18661 ataacgatgc gtaagtcagt tatcgcgatt atcatcatcg tgctggtagt gctttacatg
    18721 tctgtctttg tcgtcaaaga aggtgagcgc ggtattacgc tgcgttttgg taaggtactg
    18781 cgtgacgatg acaacaaacc tttggtttat gagcctggtc tgcatttcaa gataccgttc
    18841 attgaaacgg tgaaaatgct cgacgcacgt attcagacca tggacaacca ggccgaccgc
    18901 tttgtgacca aagagaagaa agacctgatc gtcgactctt acatcaaatg gcgcatcagc
    18961 gatttcagcc gttactacct ggcaacgggt ggtggcgaca tttcgcaagc ggaagtgctg
    19021 ttgaaacgta agttctctga ccgtctgcgt tctgaaattg gtcgcctgga cgtgaaagat
    19081 atcgtcaccg attcccgtgg tcgtctgacc ctcgaagtac gtgacgcgct gaactccggt
    19141 tctgcgggta cagaagatga agttactacc ccggcagcag ataacgccat tgccgaagcg
    19201 gcagagcgcg taacggctga gacgaagggc aaagttccgg tcatcaaccc gaacagtatg
    19261 gcggcgctgg gtattgaagt tgtcgatgtg cgtatcaagc agatcaacct gccgaccgaa
    19321 gtgtctgaag cgatctacaa ccgtatgcgc gccgagcgtg aagcggtagc gcgtcgtcac
    19381 cgttcacaag gtcaggaaga agcggaaaaa ctgcgcgcga ctgccgacta tgaagtgacc
    19441 agaacgctgg cagaagctga gcgtcagggc cgcattatgc gtggtgaagg cgatgccgaa
    19501 gcagccaaac tgtttgctga tgcattcagt aaagatccgg acttctacgc attcatccgt
    19561 agcctgcgtg cttatgagaa cagcttctct ggcaatcagg acgtgatggt catgagcccg
    19621 gatagcgatt tcttccgcta catgaagacg ccgacttccg caacgcgtta atataacgac
    19681 tgcggtacag gtcaataaag ccaccgcatc ctcagggatg tcggtggttt tctttttcta
    19741 taaggataat gaatgaattc gacaatctgg ctggcgcttg ccctggtttt ggtactggaa
    19801 ggtttagggc cgatgcttta cccgaaggca tggaagaaga tgatctctgc gatgaccaat
    19861 ttgcccgata atattttacg tcgttttggc ggtggacttg tggttgcggg cgttgtggtc
    19921 tactacatgt tgaggaaaac gattggctga acaaaaaaca gactgatcga ggtcattttt
    19981 gagtgcaaaa agtgctgtaa ctctgaaaaa gcgatggtag aatccatttt taagcaaacg
    20041 gtgattttga aaaatgggta acaacgtcgt cgtactgggc acccaatggg gtgacgaagg
    20101 taaaggtaag atcgtcgatc ttctgactga acgggctaaa tatgttgtac gctaccaggg
    20161 cggtcacaac gcaggccata ctctcgtaat caacggtgaa aaaaccgttc tccatcttat
    20221 tccatcaggt attctccgcg agaatgtaac cagcatcatc ggtaacggtg ttgtgctgtc
    20281 tccggccgcg ctgatgaaag agatgaaaga actggaagac cgtggcatcc ccgttcgtga
    20341 gcgtctgctg ctgtctgaag catgtccgct gatccttgat tatcacgttg ctctggataa
    20401 cgcgcgtgag aaagcgcgtg gcgcgaaagc gatcggcacc accggtcgtg gtatcgggcc
    20461 tgcttatgaa gataaagtgg cacgtcgcgg tctgcgtgtt ggcgaccttt tcgacaaaga
    20521 aaccttcgct gaaaaactga aagaagtgat ggaatatcac aacttccagt tggttaacta
    20581 ctacaaagct gaagcggttg attaccagaa agttctggat gatacgatgg ctgttgccga
    20641 catcctgact tctatggtgg ttgacgtttc tgacctgctc gaccaggcgc gtcagcgtgg
    20701 cgatttcgtc atgttcgaag gtgcgcaggg tacgctgctg gatatcgacc acggtactta
    20761 tccgtacgta acttcttcca acaccactgc tggtggcgtg gcgaccggtt ccggcctggg
    20821 cccgcgttat gttgattacg ttctgggtat cctcaaagct tactcaactc gtgtaggtgc
    20881 aggtcctttc ccggccgaac tgtttgatga aactggcgag ttcctctgca agcagggtaa
    20941 cgaattcggt gcaactacgg gtcgtcgtcg tcgtaccggc tggctggaca ccgttgccgt
    21001 tcgtcgtgcg gtacagctga actccctgtc tggcttctgc ctgactaaac tggacgttct
    21061 ggatggcctg aaagaggtta aactctgcgt ggcttaccgt atgccggatg gtcgcgaagt
    21121 gactaccact ccgctggcag ctgacgactg gaaaggtgta gagccgattt acgaaaccat
    21181 gccgggctgg tctgaatcca ctttcggcgt gaaagatcgt agcggcctgc cgcaggcggc
    21241 gctgaactac atcaagcgta ttgaagagct gaccggtgtg ccgatcgata tcatctctac
    21301 cggtccggat cgtactgaaa ccatgattct gcgcgacccg ttcgacgcgt aattctggta
    21361 cgcctggcag atattttgcc tgccgggcta acagtgtgat acattgctgt gtcggttaag
    21421 ccattacgct atccgacaca gtgttaaatc ctcgcttttt tccttccccg aactgaaata
    21481 aattagcgac acagcttgtg gctggtttat catcaatata aatgtatttt ttcccgattt
    21541 cccttttgag gttgatgtgc agttaacgag tttcactgat tacggattac gtgcgctgat
    21601 ctacatggcg tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta
    21661 cggcgtctcc cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt
    21721 gactgctgtt cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg
    21781 tattggtgat gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt
    21841 ttgccacatt acacctgcct gtcggttgaa acaggcgctt tctaaggccg tgcaaagttt
    21901 tcttacggaa ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta
    21961 taaattattg ctggtggagt gacgaaaatc ttcatcagag atgacaacgg aggaaccgag
    22021 atgtcacaag atcctttcca ggaacgcgaa gctgaaaaat acgcgaatcc catccctagt
    22081 cgggaattta tcctcgaaca tttaaccaaa cgtgaaaaac cggccagccg tgatgagctg
    22141 gcggtagaac tgcacattga aggcgaagag cagcttgaag gcctgcgtcg ccgcctgcgc
    22201 gcgatggagc gcgatggtca actggtcttc actcgtcgtc agtgctatgc gctgccggaa
    22261 cgcctcgacc tggtgaaagg taccgttatt ggtcaccgtg atggctacgg ctttctgcgg
    22321 gttgaagggc gtaaagatga tttgtatctc tccagcgagc agatgaaaac ctgcattcat
    22381 ggcgatcagg tgctggcgca gccgctgggt gctgaccgta aaggtcgtcg tgaagcgcgt
    22441 attgtccgcg tactggtgcc aaaaaccagc cagattgttg gtcgctactt taccgaagcg
    22501 ggcgtcggct ttgtggttcc tgacgatagc cgtctgagct tcgatatctt aatcccgccc
    22561 gatcagatca tgggcgcgcg gatgggcttt gtggtcgtag tcgaactgac tcagcgtccg
    22621 actcgccgca ccaaagcggt gggtaaaatc gtcgaagtgc tgggcgacaa tatgggcacc
    22681 ggcatggcgg ttgatatcgc tctgcgtacc catgaaattc cgtatatctg gccgcaggct
    22741 gttgagcaac aggttgcggg gctgaaagaa gaagtgccgg aagaagcaaa agcgggccgt
    22801 gtcgatttgc gcgatttacc gctggtcacc attgatggcg aagacgcccg tgactttgac
    22861 gatgcagttt actgcgagaa aaaacgcggc ggcggctggc gtttatgggt cgcgattgcc
    22921 gacgtcagct actatgtgcg tccgccaacg ccgctggaca gagaagcgcg taaccgtggc
    22981 acgtcggtgt acttcccttc gcaggttatc ccgatgctgc cggaagtgct ctctaacggc
    23041 ctgtgttcgc tcaacccgca ggtagaccgc ctgtgtatgg tgtgcgagat gacggtttcg
    23101 tcgaaaggcc gcctgacggg ctacaaattc tacgaagcgg tgatgagctc tcacgcgcgt
    23161 ctgacctaca ccaaagtctg gcatattctg cagggcgatc aggatctgcg cgagcagtac
    23221 gccccgctgg ttaagcatct cgaagagttg cataacctct ataaagtgct ggataaagcc
    23281 cgtgaagaac gcggtgggat ctcatttgag agcgaagaag cgaagttcat tttcaacgct
    23341 gaacgccgta ttgaacgtat cgaacagacc cagcgtaacg acgcgcacaa attaattgaa
    23401 gagtgcatga ttctggcgaa tatctcggcg gcgcgtttcg ttgagaaagc gaaagaaccg
    23461 gcactgttcc gtattcacga caagccgagc accgaagcga ttacctcttt ccgttcagtg
    23521 ctggcggagc tggggctgga gctgccgggt ggtaacaagc cggaaccgcg tgactacgcg
    23581 gaactgctgg agtcggttgc cgaccgtcct gatgcagaaa tgctgcaaac catgctgcta
    23641 cgctcgatga aacaggcgat ttacgatcca gaaaaccgtg gtcacttcgg tctggcattg
    23701 cagtcctatg cgcactttac ttcgccgatt cgtcgttatc ctgacctgac gctgcaccgc
    23761 gccattaaat atctgctggc gaaagagcag gggcatcagg gcaacaccac tgaaaccggc
    23821 ggctaccatt attcgatgga agagatgttg caactgggtc agcactgttc gatggcggaa
    23881 cgtcgtgccg acgaagcaac gcgcgatgtc gctgactggc tgaagtgtga cttcatgctc
    23941 gaccaggtag gtaacgtctt taaaggcgta atttccagcg tcactggctt tggcttcttc
    24001 gtccgtctgg acgacttgtt cattgatggt ctggtccatg tctcttcgct ggacaatgac
    24061 tactatcgct ttgaccaggt agggcaacgc ctgatggggg aatccagcgg ccagacttat
    24121 cgcctgggcg atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc
    24181 gactttagtc tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag
    24241 aaagcgaaaa aaggcgatgc aggcaaaaaa ggcggcaagc gtcgtcaggt cggtaaaaag
    24301 gtaaactttg agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg
    24361 aagaaagacg cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg
    24421 accaaagcga agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa
    24481 aaagagggtt agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc
    24541 ttatccggcc tacataatca cgcaaattca atatattgca gagatcatgt aggccggata
    24601 aacgtagtgc atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg
    24661 gcatccacgc agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta
    24721 ttttaaaagg ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg
    24781 gcgtggttat ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc
    24841 atcagggcat tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg
    24901 atctgatcgc ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc
    24961 acaacctcgg cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc
    25021 cgaaagatcg ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag
    25081 aaagcgttcc gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag
    25141 agaatatctg gatcgtcggt acggcaggcg aggcggatca tacactctat cagagcaaaa
    25201 tgaccggacg cctggcgctg gtgatgggcg cggaaggtga aggtatgcgt cgcctgaccc
    25261 gtgaacattg cgatgagttg atcagcatcc caatggcagg aagtgtttct tccctgaacg
    25321 tttcggttgc aaccggaatt tgcttatttg aagcggttcg tcagcgcagc tagttcctct
    25381 gaattatgaa aagccatcca gatttggatg gttt
//
LOCUS       DAJTNW010000061        24971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNW010000061 DAJTNW010000000
VERSION     DAJTNW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..465
                     /locus_tag="OO179_002554"
                     /pseudo
     CDS             1..465
                     /locus_tag="OO179_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            793..1875
                     /locus_tag="OO179_002555"
     CDS             793..1875
                     /locus_tag="OO179_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5532571.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2364..3162)
                     /locus_tag="OO179_002556"
                     /pseudo
     CDS             complement(2364..3162)
                     /locus_tag="OO179_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO179_002557"
     CDS             complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO179_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5532572.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO179_002558"
     CDS             complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO179_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5532573.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO179_002559"
     CDS             complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO179_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5532574.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5097..6762
                     /gene="fliF"
                     /locus_tag="OO179_002560"
                     /pseudo
     CDS             5097..6762
                     /gene="fliF"
                     /locus_tag="OO179_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6755..7750
                     /gene="fliG"
                     /locus_tag="OO179_002561"
     CDS             6755..7750
                     /gene="fliG"
                     /locus_tag="OO179_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5532575.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7743..8429
                     /gene="fliH"
                     /locus_tag="OO179_002562"
     CDS             7743..8429
                     /gene="fliH"
                     /locus_tag="OO179_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5532576.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8429..9802
                     /gene="fliI"
                     /locus_tag="OO179_002563"
     CDS             8429..9802
                     /gene="fliI"
                     /locus_tag="OO179_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5532577.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9824..10165
                     /gene="fliJ"
                     /locus_tag="OO179_002564"
                     /pseudo
     CDS             9824..10165
                     /gene="fliJ"
                     /locus_tag="OO179_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10162..11289
                     /gene="fliK"
                     /locus_tag="OO179_002565"
     CDS             10162..11289
                     /gene="fliK"
                     /locus_tag="OO179_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5532578.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11394..11858
                     /gene="fliL"
                     /locus_tag="OO179_002566"
     CDS             11394..11858
                     /gene="fliL"
                     /locus_tag="OO179_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5532579.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11863..12867
                     /gene="fliM"
                     /locus_tag="OO179_002567"
     CDS             11863..12867
                     /gene="fliM"
                     /locus_tag="OO179_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5532580.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12864..13277
                     /gene="fliN"
                     /locus_tag="OO179_002568"
     CDS             12864..13277
                     /gene="fliN"
                     /locus_tag="OO179_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5532581.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13280..13645
                     /gene="fliO"
                     /locus_tag="OO179_002569"
     CDS             13280..13645
                     /gene="fliO"
                     /locus_tag="OO179_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5532582.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13645..14382
                     /gene="fliP"
                     /locus_tag="OO179_002570"
     CDS             13645..14382
                     /gene="fliP"
                     /locus_tag="OO179_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5532583.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            14392..14661
                     /gene="fliQ"
                     /locus_tag="OO179_002571"
     CDS             14392..14661
                     /gene="fliQ"
                     /locus_tag="OO179_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5532584.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14670..15455
                     /gene="fliR"
                     /locus_tag="OO179_002572"
     CDS             14670..15455
                     /gene="fliR"
                     /locus_tag="OO179_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5532585.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15740..16363
                     /gene="rcsA"
                     /locus_tag="OO179_002573"
     CDS             15740..16363
                     /gene="rcsA"
                     /locus_tag="OO179_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5532586.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16407..16595)
                     /gene="dsrB"
                     /locus_tag="OO179_002574"
     CDS             complement(16407..16595)
                     /gene="dsrB"
                     /locus_tag="OO179_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5532587.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16758..16985
                     /gene="yodD"
                     /locus_tag="OO179_002575"
     CDS             16758..16985
                     /gene="yodD"
                     /locus_tag="OO179_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5532588.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17283..18098
                     /locus_tag="OO179_002576"
                     /pseudo
     CDS             17283..18098
                     /locus_tag="OO179_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18095..19789)
                     /gene="yedQ"
                     /locus_tag="OO179_002577"
                     /pseudo
     CDS             complement(18095..19789)
                     /gene="yedQ"
                     /locus_tag="OO179_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19960..20142)
                     /locus_tag="OO179_002578"
     CDS             complement(19960..20142)
                     /locus_tag="OO179_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5532589.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20221..21138)
                     /locus_tag="OO179_002579"
     CDS             complement(20221..21138)
                     /locus_tag="OO179_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5532590.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21311..22231
                     /gene="yedA"
                     /locus_tag="OO179_002580"
     CDS             21311..22231
                     /gene="yedA"
                     /locus_tag="OO179_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5532591.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22220..22690)
                     /gene="vsr"
                     /locus_tag="OO179_002581"
     CDS             complement(22220..22690)
                     /gene="vsr"
                     /locus_tag="OO179_002581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5532592.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22671..24089)
                     /gene="dcm"
                     /locus_tag="OO179_002582"
     CDS             complement(22671..24089)
                     /gene="dcm"
                     /locus_tag="OO179_002582"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5532593.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24156..24696)
                     /locus_tag="OO179_002583"
                     /pseudo
     CDS             complement(24156..24696)
                     /locus_tag="OO179_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24736..>24971)
                     /locus_tag="OO179_002584"
     CDS             complement(24736..>24971)
                     /locus_tag="OO179_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532594.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPACCRRYE"
BASE COUNT         6309 a         6409 c         6153 g         6100 t
ORIGIN      
        1 tgttaattaa ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa
       61 ggcgtatcgg gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc
      121 aaagatgttt tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc
      181 gtcatccgcc aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta
      241 tatccccgcc ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac
      301 acctgcatgg acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa
      361 agtattagag ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa
      421 aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac
      481 attaataaat ctaaataaat atcatgcaaa taatttccat ggttattttt aggctattta
      541 aattaaaaaa ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac
      601 caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata
      661 aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
      721 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
      781 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
      841 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
      901 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
      961 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
     1021 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
     1081 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
     1141 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
     1201 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
     1261 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
     1321 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
     1381 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
     1441 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
     1501 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
     1561 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
     1621 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
     1681 ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca
     1741 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
     1801 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
     1861 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
     1921 atataagtgg cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata
     1981 aattaccctt tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca
     2041 attaacgtat caattagatt taataacaac gatatccgta cctaacctga tatctaatgt
     2101 cataagcatc cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca
     2161 catagcccaa acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag
     2221 gaagtgagta tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga
     2281 ttgttcaggc tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc
     2341 tgttgctcct taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
     2401 atattaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
     2461 ctggcaatct cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa
     2521 cctcacgtcg tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa
     2581 tgtggaaaca gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg
     2641 ttcggatagc attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag
     2701 taaacaatta acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg
     2761 aatttgcgtt gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa
     2821 ctcggggttc tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata
     2881 aaatttttga aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag
     2941 acctgcttct ggataattca caatataaaa ttcatatcca cagggaaata atattatctg
     3001 attattatca acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat
     3061 acgacataaa gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg
     3121 tatttcttca atctttaggt tagacttatc tcttgccagc atactcttac cctattttat
     3181 ctcataaact ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac
     3241 taacgctccg ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt
     3301 aaactggtaa tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata
     3361 accaaaatca tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc
     3421 tgcatcagtg ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca
     3481 tccactgcct gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc
     3541 aggagcacca gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga
     3601 aaatacatga gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa
     3661 tgctgcggcc tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt
     3721 cataaaaacc tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta
     3781 tgaataaagg cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt
     3841 agccataata tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg
     3901 tgcacttcgt gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat
     3961 gatagctggc aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac
     4021 gataccgact cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg
     4081 agctaataac cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg
     4141 tgtaaacccc tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc
     4201 accaagataa atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt
     4261 actacagttt gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct
     4321 ggcgtggcaa gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa
     4381 tgagttcact cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg
     4441 tggatggcag aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt
     4501 gccccatcca tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt
     4561 tacacaacct acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc
     4621 acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt
     4681 agcgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt
     4741 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc
     4801 ggcagtgatt cctgcgcaca cgcactcatc gccgtagcct gtaactggct gataacccct
     4861 tcaatcccct gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac
     4921 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat
     4981 agaaaaaacg aaagcacaaa taatgggagc gtcaattttt cgagtttgct gacccgagag
     5041 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga
     5101 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
     5161 aaattccatt gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
     5221 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
     5281 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
     5341 ctattgaggt tccggcagat aaagttcacg aactgcgcct gcgcctggcg caacaaggtt
     5401 tgcccaaagg cggcgcggtc ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc
     5461 agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg
     5521 aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat
     5581 tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg
     5641 cactcgatga agggcaaatt agcgctattg tacatctggt ttccagcgcg gttgctggtc
     5701 tgccgcctgg aaacgtcacg ctggtggatc agggcgggca tctgttaacc cagtccaata
     5761 ccagcgggcg cgatcttaat gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta
     5821 ttcagcagcg tattgaagcg atcctgtcac ctattgttgg taacggtaat attcacgcct
     5881 aggtcacggc gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta
     5941 acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg
     6001 gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata
     6061 acgcgccaat cagcacgcct ccgacaaatc aaaataaccg ccagcagcag gcgagcacca
     6121 ccagcaatag tgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc
     6181 gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag
     6241 tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga
     6301 agcaaattga agctctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc
     6361 tcaatgttgt taactcgccg ttcaatagca gtgacgaaag cggcggagca ctgccgtttt
     6421 ggcaacagca agtgtttatc gatcagttgc tggctgccgg tcgctggttg ctggttgctg
     6481 gtactgctgg tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc
     6541 gccgaggcgg tgaagactgt gcagcaacag gcgcaggccc acgaggaagt ggaagatgcg
     6601 gtggaagtcc gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg
     6661 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     6721 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     6781 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     6841 ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     6901 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgctgcact
     6961 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg
     7021 tgccgccagc ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac
     7081 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     7141 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     7201 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     7261 agccgcgctg gccgaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     7321 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca
     7381 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
     7441 catcgacgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg
     7501 tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc
     7561 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     7621 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct
     7681 gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg attggcagcg gcgaggatac
     7741 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg
     7801 cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc
     7861 ttgagcagca actggcgcaa ctgcaaatgc aggcccacga gcagggttat caggcgggga
     7921 ttgccgaagg tcgccagcaa ggtcacgaac agggctatca ggaaggactg gcccaggggc
     7981 tggaacaagg tctggcagag gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc
     8041 aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgata gcttcgcgcc
     8101 tgatgcagat ggcgctggag gcagcacgtc aggtgatcgg tcagacacca acggtggata
     8161 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggga
     8221 aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa
     8281 ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccaccct ggcggatgta
     8341 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     8401 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     8461 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     8521 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     8581 ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     8641 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg
     8701 cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca agcagttgcc
     8761 gctcggtccg gcgttattag gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct
     8821 gcccttcccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
     8881 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     8941 cgtcggacgc gggcagcgta tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct
     9001 gctggggatg atggcacgct acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     9061 acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
     9121 ctcagtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc
     9181 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     9241 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     9301 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     9361 tgccggtaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
     9421 aggcgatgac cagcaggacc ccattgctga ctccgcgcgg gcaatcctcg acggtcacat
     9481 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     9541 gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     9601 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     9661 caaaggcagc gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta
     9721 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     9781 tattttcccg acagtgtcat aacccaggag ataacggcag atggcggaag aacagctcaa
     9841 aatgttgatc gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg
     9901 gatgaccagc aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat
     9961 tactcagcat cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag
    10021 ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac
    10081 ggcggcactg cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg
    10141 cgccgccatg aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac
    10201 accaccacat tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg
    10261 agcgaagcat tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtagca
    10321 acagataagc ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa
    10381 caagcggatt tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc
    10441 acatcaacac cgttaaccac cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat
    10501 acgacaaaag acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc
    10561 ctttttgcga tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc
    10621 gtgttaccgg cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca
    10681 gcacagcctg atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa
    10741 gcccagagta aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg
    10801 ctaatcactc cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca
    10861 ccgctgggtt ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc
    10921 caggggcaac aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt
    10981 tccctcaaag tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta
    11041 cgcgccgccc tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt
    11101 cagttagggc aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc
    11161 cagcaacagc aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat
    11221 acgcttccgg ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt
    11281 ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca
    11341 agacgcagga taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg
    11401 attacgcgat aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta
    11461 ccctcgcggc ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg
    11521 acgacaaagc gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta
    11581 cggtcaattt gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag
    11641 atgaagcaac ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt
    11701 tactgttttc acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga
    11761 ttgccgagat taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg
    11821 tcaccgacgt gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt
    11881 tctcaagctg aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca
    11941 gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt
    12001 gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt
    12061 atggggctgt tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt
    12121 cagccgtacc atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac
    12181 ctgaaaccgc tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc
    12241 gtggataacc tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt
    12301 acccatactg aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc
    12361 gacgcctgga aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg
    12421 gaatttacca atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg
    12481 gagattggca acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg
    12541 ctacgggaat tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg
    12601 cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc
    12661 gatatctcgc tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata
    12721 gaaaaacccg atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac
    12781 ggtactctca acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat
    12841 tctctgaacg aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg
    12901 gcgcaatgga cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa
    12961 gcgctgccga cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg
    13021 atatcgacct gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga
    13081 tgaccatcaa agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg
    13141 cgggcgaacc gctggatatt ctgatcaacg gttatttaat cgcccagggc gaagtggtgg
    13201 ttgttgccga taaatatggc gtgcggatca ccgatatcat taccccgtcc gagcggatgc
    13261 gccgcctgag ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc
    13321 tgctgagcca ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc
    13381 tgctgcctgg ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct
    13441 gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga
    13501 agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc
    13561 cccttcagca ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt
    13621 gcttaagcgt ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc
    13681 tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct
    13741 ggcggtggac aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg
    13801 ttcattccag cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt
    13861 ttattgcgta acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg
    13921 gcactgtttt tgaccttttt tattatgtcg ccagtgatca acaaaattta tgtcgatgcg
    13981 taccagccat tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag
    14041 ccgctgcgtg agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga
    14101 ctggcgaata ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg
    14161 gcctacgtga ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct
    14221 tttttgatta tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt
    14281 ccccccgcca ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg
    14341 caattgctgg tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct
    14401 gaatcggtca tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg
    14461 ctgttgctgg tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag
    14521 attaacgaaa tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt
    14581 attgccggac cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac
    14641 ctgccgtata tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt
    14701 cctggttaag cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc
    14761 caattctgag cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca
    14821 cgttcgccat tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc
    14881 tgtggctggc cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg
    14941 cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg
    15001 cgacatttgt cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata
    15061 tgctggcatt actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg
    15121 tcgatacctt tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg
    15181 cacccaccaa agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca
    15241 ttactctgtt gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat
    15301 tatccatttt tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg
    15361 cattaatgcc gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc
    15421 tggctgatat tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc
    15481 taaggattat ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat
    15541 atttgtttta agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt
    15601 atacaacact ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt
    15661 gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg
    15721 cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact
    15781 aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga
    15841 aaccgttgat gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa
    15901 tgaggactgt ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca
    15961 acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata
    16021 tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga
    16081 cgatatcctt ggcaatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc
    16141 gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg
    16201 aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg
    16261 taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact
    16321 gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtga
    16381 tttcccacca gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga
    16441 tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcgc aaccaggtac
    16501 attgtgcctt cactaaactc ctcaactgcc agtaccactc caggacgacg cggaccgcca
    16561 tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc
    16621 agtgtagaac aatttcgttt ttctggcagc gccaagcgcg cgcgagtgct gattttctcg
    16681 acggtctata cttaagagat gctaggcgga cttaacgact ggcggcaaca acagagtaac
    16741 ggttgcgagg aaagatgatg aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg
    16801 tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc
    16861 atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc
    16921 gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc
    16981 gttgaattca caataaaaaa atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc
    17041 aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc
    17101 aatttttttc gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata
    17161 gaaatgtgac gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa
    17221 atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga
    17281 ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg
    17341 acagtcatag ttatgactgg caaccggcag ccccctggct cagccgttta cacgaagcca
    17401 atattcccgt cattctctgt agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa
    17461 tgttggggct acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg
    17521 aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa
    17581 tctgccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg
    17641 atgtcgacga tgcaaccatt gccgaatgga cgggattaag ccgtagctag gcggcgctga
    17701 cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac
    17761 aatttatcgc tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc
    17821 acgtactgga tgcctctgcc ggaaaagatc aggctgccaa ttggattatc gcgacctatc
    17881 aacaattgtc aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc
    17941 ctttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac
    18001 atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct
    18061 ggcgtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc
    18121 gattgcgccc ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt
    18181 caaaatcata atcaccggtt tcctcgctgc tacttacccc cagcgaggca ctgatgcgta
    18241 tcgttgtact cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg
    18301 cgacctccgc agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac
    18361 cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag
    18421 aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa
    18481 gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac
    18541 gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct
    18601 gccactgcaa cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc
    18661 aggagaggag taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac
    18721 tgccgaaatc gccgcgcacg ccttcgctta gcgtatggac gcgcgccagc acaccgtcga
    18781 aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt
    18841 catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg
    18901 gatggtcagg attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac
    18961 catcgaggtt tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac
    19021 tcatccccag cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct
    19081 gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac
    19141 gattttctcg ttgcgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg
    19201 ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga
    19261 catacatcgc ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa
    19321 cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggaaattcc
    19381 cctcgctgac taatgcatcc gatacgccat tgactggcag ggtgcgccgt cggttgagtt
    19441 cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgcagag
    19501 aggtgaaatc cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt
    19561 tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac
    19621 gctagctgga gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg
    19681 aaaaaagcaa taccacgata aagcagagat taacgacatg accgggcccc aggcggcgtg
    19741 cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc
    19801 ccaacctgct tttttatgat tctggcataa cccggcaata gcgtaccgca aaacagcgat
    19861 aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca ttgggacacc
    19921 aggccggatg tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg
    19981 actcccctcc cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc
    20041 gtcataccaa cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc
    20101 gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg
    20161 agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta
    20221 ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact cccagcacca cgatgccgcc
    20281 gatgataaat ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact
    20341 ttgctgcccg gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat
    20401 cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca
    20461 aggcgcgata atcaataacc ctttacctaa tgcctgcatc agcgcgctag atttttccgc
    20521 cagccaatac cctaggtcat caatcttaac gataacccct accagaccgt agacgcccac
    20581 ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc
    20641 cacaatcccc agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc
    20701 ccctttaatt ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg
    20761 ctgctggctc tgcgctggat cttctttatg tttacgcgcc tccagcatat gcagcacttt
    20821 ctctacccct tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca
    20881 cgggataaac gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga
    20941 tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg
    21001 ttgcgcattg agcgataaat catcccctaa tacaccggcg gttttcttcg ccgccagttt
    21061 gcccatcaca gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact
    21121 actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa
    21181 aaacggcagc tctgatactt gcttcacagg tcaacattta tttaacaatc aagtgcaatt
    21241 atatctctcg ccagcagaat gattttcacg tttactatga accacctttt tatttccgcc
    21301 gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta
    21361 tatcatttgg ggctcaacct attttgtcat tcggattggc gtgggaagct ggcctccgtt
    21421 aatgatggcg ggcgttcgat tccaggcagc cggtatttta ttgctggcat ttttgctact
    21481 gcgcggacac aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt
    21541 attgctggct gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg
    21601 catcgccgcc gtagtgattg caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt
    21661 tggcattaaa acgcgcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat
    21721 catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat
    21781 cggctcgatt agctgggcgt ttggctcagt ttatggctcg cgcattacct tacctgtagg
    21841 gatgatggcg ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat
    21901 gattgcgggt gaaaaactga cggcgctccc ttccctgtca ggcttccttg cggtcggcta
    21961 tctggcgctg tttggttcga ttatcgccat taacgcttat atgtatttaa tccgtaatgt
    22021 cagtccggct ctcgccacca gctacgccta cgttaacccg gtggtcgcgg tcttgctggg
    22081 tacgggactg ggtggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt
    22141 tttcgcggtg gtactggtca cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc
    22201 gccagttatt caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc
    22261 tggcaccttc gccgcagatc cactcttcca gacgctcggt aagcgccgca tccgtcagct
    22321 tctcgcgcct acgtaacgca cactcccaga caatcagtac gcgccagccg agctcctgca
    22381 agcgactgat atcgcggcga tcgcgttcaa catttttacc tatcttctcc agccagaact
    22441 cagttcgagt cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt
    22501 gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg
    22561 cgtcctgaac gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa
    22621 tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa
    22681 cgtcgaccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca
    22741 agcagttttg ccaccgcggc aaagaccggc acgaccaccg agttgccgaa ctggcgatag
    22801 gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt
    22861 aagcgcgcgc attcacgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg
    22921 tcaaagtctt tctcacccgt ggccatatcc caaccgcgat cgattaaaat ttccgcgcca
    22981 tctttgtaat aacgagcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc
    23041 attccataac cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagaggtac
    23101 ttccacagca ccggcgtcag gatatatttc gcctcaacca tcgggtccag cagctgcgcc
    23161 agcgtcactc gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc
    23221 agattcagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa
    23281 tgtttaccat cgatgatttt cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca
    23341 tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactc
    23401 ttcaggtttt tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc
    23461 accacatcaa agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc
    23521 gagttctttt tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa
    23581 acatcgtgtt caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct
    23641 tcttgatgac tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg
    23701 caataatggt tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg
    23761 cactgtccac caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg
    23821 ataaagcgaa acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga
    23881 tgggcaaact ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt
    23941 aaaattgccg cgctccagtg attctcaccc acaccattaa gctgcgccac cagcatttta
    24001 acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat
    24061 gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca
    24121 ggttaacata attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct
    24181 atcaccttgt gatcgatacc ttcattctcc cccgccagtt cggcaccgag cttcgccatc
    24241 aaacagcgcc aagctcaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc
    24301 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    24361 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg
    24421 aaattgcacg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    24481 acgctgccgt tgcggatgat ttttcgccag gctcacgata tcgtgaaaat aacaggcggt
    24541 taaaatcacc agcatatcga catcgtcatc tgccgccagt ttctgtgccg tagcccagac
    24601 acggcgaaaa tgacacaggt catgcgccgc gtcctgatgt tgatgatggt ttttcaacca
    24661 gttctcgaac tgtgcctgcc agtgttgtaa ctccatacgc gctccgttgc caaagagtcg
    24721 caaccttagc agtttttatt catagcgtct gcaacatgcg ggacgaggtc gggtgtacca
    24781 tgcaatgccg cctaacaagg caccaaccag ggccataaac agaaatccga ccaatgctcc
    24841 taaccacttc cccgcggtag atcctaaatc actttcaaat cccgacacgg gggcatcggg
    24901 gatccggctc atcacccaga tgtatcgcgc cattgcccag acgaaatagc cacaccatgt
    24961 atagaaagcc c
//
LOCUS       DAJTNW010000062        24600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNW010000062 DAJTNW010000000
VERSION     DAJTNW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(1..374)
                     /locus_tag="OO179_002585"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OO179_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="OO179_002586"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="OO179_002586"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5532595.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="OO179_002587"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="OO179_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5532596.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="OO179_002588"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="OO179_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5532597.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="OO179_002589"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="OO179_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5532598.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="OO179_002590"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="OO179_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5532599.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="OO179_002591"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="OO179_002591"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5532600.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="OO179_002592"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="OO179_002592"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5532601.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="OO179_002593"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="OO179_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5532602.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="OO179_002594"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="OO179_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5532603.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="OO179_002595"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="OO179_002595"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5532604.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="OO179_002596"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="OO179_002596"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5532605.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="OO179_002597"
     CDS             10736..11161
                     /locus_tag="OO179_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5532606.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="OO179_002598"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="OO179_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5532607.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="OO179_002599"
     CDS             12185..13621
                     /locus_tag="OO179_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5532608.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OO179_002600"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OO179_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5532609.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OO179_002601"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OO179_002601"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5532610.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OO179_002602"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OO179_002602"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5532611.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="OO179_002603"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="OO179_002603"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5532612.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OO179_002604"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OO179_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5532613.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="OO179_002605"
     CDS             complement(20454..20747)
                     /locus_tag="OO179_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5532614.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="OO179_002606"
     CDS             complement(20744..22033)
                     /locus_tag="OO179_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5532615.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="OO179_002607"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="OO179_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="OO179_002608"
     CDS             complement(23047..23811)
                     /locus_tag="OO179_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5532616.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="OO179_002609"
     CDS             24127..>24600
                     /locus_tag="OO179_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5532617.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6094 a         5940 c         6464 g         6102 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DAJTNW010000063        24180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNW010000063 DAJTNW010000000
VERSION     DAJTNW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(22..594)
                     /locus_tag="OO179_002610"
     CDS             complement(22..594)
                     /locus_tag="OO179_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5532618.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO179_002611"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO179_002611"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5532619.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO179_002612"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO179_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5532620.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO179_002613"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO179_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5532621.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO179_002614"
     CDS             complement(3556..5337)
                     /locus_tag="OO179_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5532622.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO179_002615"
     CDS             complement(5330..7102)
                     /locus_tag="OO179_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5532623.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO179_002616"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO179_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5532624.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO179_002617"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO179_002617"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5532625.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8661..10353
                     /locus_tag="OO179_002618"
                     /pseudo
     CDS             8661..10353
                     /locus_tag="OO179_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="OO179_002619"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="OO179_002619"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5532626.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OO179_002620"
     CDS             complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OO179_002620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5532627.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="OO179_002621"
     CDS             complement(11657..12028)
                     /locus_tag="OO179_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5532628.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OO179_002622"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OO179_002622"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5532629.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OO179_002623"
     CDS             complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OO179_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5532630.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OO179_002624"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OO179_002624"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5532631.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OO179_002625"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OO179_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5532632.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OO179_002626"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OO179_002626"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5532633.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     WRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OO179_002627"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OO179_002627"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5532634.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OO179_002628"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OO179_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5532635.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="OO179_002629"
     CDS             21799..22377
                     /locus_tag="OO179_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5532636.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..23896
                     /gene="ampG"
                     /locus_tag="OO179_002630"
     CDS             22421..23896
                     /gene="ampG"
                     /locus_tag="OO179_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5532637.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6663 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcattcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgctag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccatt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt
    22801 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    22861 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc
    22921 agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg
    22981 tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct
    23041 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    23101 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    23161 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct
    23221 tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc
    23281 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    23341 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    23401 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    23461 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta
    23521 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    23581 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    23641 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    23701 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac
    23761 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    23821 tttagtcgcc ctttctggcg tcatgcttgg tggtttgctg gattatctgg cgctacgaaa
    23881 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    23941 tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt
    24001 ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt
    24061 ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt
    24121 tatttcgcat tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta
//
LOCUS       DAJTNW010000064        23376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNW010000064 DAJTNW010000000
VERSION     DAJTNW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            78..1574
                     /gene="ravA"
                     /locus_tag="OO179_002631"
     CDS             78..1574
                     /gene="ravA"
                     /locus_tag="OO179_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5532638.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1568..3019
                     /gene="viaA"
                     /locus_tag="OO179_002632"
     CDS             1568..3019
                     /gene="viaA"
                     /locus_tag="OO179_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5532639.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3024..4016)
                     /gene="asnA"
                     /locus_tag="OO179_002633"
     CDS             complement(3024..4016)
                     /gene="asnA"
                     /locus_tag="OO179_002633"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5532640.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4168..4626
                     /gene="asnC"
                     /locus_tag="OO179_002634"
     CDS             4168..4626
                     /gene="asnC"
                     /locus_tag="OO179_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5532641.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4716..5159
                     /gene="mioC"
                     /locus_tag="OO179_002635"
     CDS             4716..5159
                     /gene="mioC"
                     /locus_tag="OO179_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5532642.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5538..7427
                     /gene="mnmG"
                     /locus_tag="OO179_002636"
     CDS             5538..7427
                     /gene="mnmG"
                     /locus_tag="OO179_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5532643.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7491..8114
                     /gene="rsmG"
                     /locus_tag="OO179_002637"
     CDS             7491..8114
                     /gene="rsmG"
                     /locus_tag="OO179_002637"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5532644.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8731..9111
                     /gene="atpI"
                     /locus_tag="OO179_002638"
     CDS             8731..9111
                     /gene="atpI"
                     /locus_tag="OO179_002638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5532645.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9120..9935
                     /gene="atpB"
                     /locus_tag="OO179_002639"
     CDS             9120..9935
                     /gene="atpB"
                     /locus_tag="OO179_002639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5532646.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9982..10221
                     /gene="atpE"
                     /locus_tag="OO179_002640"
     CDS             9982..10221
                     /gene="atpE"
                     /locus_tag="OO179_002640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5532647.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10283..10753
                     /gene="atpF"
                     /locus_tag="OO179_002641"
     CDS             10283..10753
                     /gene="atpF"
                     /locus_tag="OO179_002641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5532648.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10768..11301
                     /gene="atpH"
                     /locus_tag="OO179_002642"
     CDS             10768..11301
                     /gene="atpH"
                     /locus_tag="OO179_002642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5532649.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11314..12855
                     /gene="atpA"
                     /locus_tag="OO179_002643"
     CDS             11314..12855
                     /gene="atpA"
                     /locus_tag="OO179_002643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5532650.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12906..13769
                     /gene="atpG"
                     /locus_tag="OO179_002644"
     CDS             12906..13769
                     /gene="atpG"
                     /locus_tag="OO179_002644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5532651.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13796..15178
                     /gene="atpD"
                     /locus_tag="OO179_002645"
     CDS             13796..15178
                     /gene="atpD"
                     /locus_tag="OO179_002645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5532652.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15199..15618
                     /gene="atpC"
                     /locus_tag="OO179_002646"
     CDS             15199..15618
                     /gene="atpC"
                     /locus_tag="OO179_002646"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5532653.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15969..17339
                     /gene="glmU"
                     /locus_tag="OO179_002647"
     CDS             15969..17339
                     /gene="glmU"
                     /locus_tag="OO179_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5532654.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17501..19330
                     /gene="glmS"
                     /locus_tag="OO179_002648"
     CDS             17501..19330
                     /gene="glmS"
                     /locus_tag="OO179_002648"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5532655.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19490..20311
                     /locus_tag="OO179_002649"
     CDS             19490..20311
                     /locus_tag="OO179_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5532656.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20298..22406
                     /locus_tag="OO179_002650"
     CDS             20298..22406
                     /locus_tag="OO179_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5532657.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22403..>23376
                     /locus_tag="OO179_002651"
     CDS             22403..>23376
                     /locus_tag="OO179_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5532658.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRK"
BASE COUNT         5925 a         5600 c         6087 g         5764 t
ORIGIN      
        1 actattttag tcaccagcaa gaaacgtcta tactcgcaat ttacgcagaa cttttgacga
       61 aaggacgcca cttcattatg gctcaccctc atttattagc ggaaagaatt tcccgcctga
      121 gcagttcgtt ggaaaagggg ctttatgaac gtagccacgc catccgcttg tgtttattag
      181 cggcattaag tggtgaaagt gtgttcctcc ttggcccgcc aggtattgcc aaaagtttga
      241 tcgcccggcg cttaaaattc gcctttcaga atgcccgcgc gtttgaatat ctgatgaccc
      301 gcttctccac gccggaagaa gtttttggtc ccctttctat tcaggcgcta aaagatgaag
      361 ggcgctatga acgtttaacc agcggttacc tgccggaagc agaaatcgtc tttctggatg
      421 agatctggaa agcgggcccg gcaattctta ataccttgct caccgccatt aacgagcgcc
      481 agttccgcaa cggtgcacac gtagaaaaaa tcccgatgcg tctactggtg gcggcctcca
      541 acgagctgcc ggaagcagac agcagtctgg aagcgttata tgaccgcatg ctgattcgtc
      601 tatggttaga taaagtgcaa gataaagcga atttccgctc catgctgacc agtcaacagg
      661 atgaaaacga caatccggtt cctgatgcct tgcaggtcac agatgaagaa tatgaacgct
      721 ggcagaaaga gattggtgaa attacgctgc ccgatcatgt atttgagctg atttttatgc
      781 tgcgccagca actggataaa ttaccggatg cgccttatgt ctcggatcgt cgctggaaaa
      841 aagcgatccg cttattgcag gccagcgcct tttttagcgg tcgcagtgct gttgccccgg
      901 ttgatctcat tttgctgaaa gattgcctgt ggtatgacgc gcaaagcctg aatttgatac
      961 aacaacaaat tgatgtattg atgaccggtc atgcctggca acagcaaggg atgttgaccc
     1021 gcctgggcgc gattgtgcaa cgtcacctgc aactccagca gcaacaaagc gataaaacag
     1081 ccttaacgtt aattcgtctg ggcggcattt tcagccgtcg tcagcagtat caacttcctg
     1141 ttaacgttac tgcttccact ctgactctgc tgctgcaaaa accgttaaaa ctgcatgata
     1201 tggaagtggt tcatatctcc tttgagcgta gcgcgctgga acagtggctg agtaaaggtg
     1261 gtgaaattcg cggcaaactg aacggtatcg gctttgccca gaaactgaat ctggaagttg
     1321 atagcaccca acatcttgtt gtacgcgatg taagtttaca aggcagtacg ctggcacttc
     1381 ccggttcatc ggctgaaggt ctgccaggtg aaataaaaca acaactggaa gagcttgaaa
     1441 gcgactggcg taagcaacac gctttattca gcgaacagca aaaatgtctg tttatccctg
     1501 gcgactggtt aggtcgcatt gaagacagcc tacaggatgt cggtgcacag attcgtcagg
     1561 cacaacaatg ctaacgctgg atacgcttaa tgtgatgctg gccgtcagcg aagagggatt
     1621 gatcgaagag atgatcatcg cactgctggc ctcaccgcag ctggcggtct tctttgaaaa
     1681 attcccacga ctgaaggcgg caatcactga tgatgttccc cgctggcgtg aggcgctgcg
     1741 cagtcggctg aaagatgccc aagtcccgcc ggaactcacc gaagaggtga tgtgctatca
     1801 gcaaagccag ctcctctcca cgccgcagtt tattgtgcag ctaccacaga tcctggactt
     1861 actgcatcgt ctgaattccc catgggcaga acaagcccgg cagttggttg atgctaacag
     1921 cacaataact tcagcgttac acacgctttt tctccagcgt tggcgtttaa gtctgatcgt
     1981 gcaagcaacg acgttaaatc aacagctatt agaagaagaa cgcgaacaac tgttaagtga
     2041 agttcaggaa cgcatgacgc tgagcggaca acttgaaccg attctcgcag ataacaatac
     2101 cgcagctggt cgtctgtggg atatgagcgc cggtcaactt aaacgtggcg actatcagtt
     2161 tattgtgaaa tacggtgaat ttcttaacga acagccggaa ctgaaacgcc tggcagagca
     2221 gctggggcgt tctcgggaag ccaaatcaat accgcgcaac gatgcgcaga tggaaacctt
     2281 ccgcaccatg gtgcgcgaac cggcgacggt tcctgagcag gttgatggtc tgcaacaaag
     2341 cgatgatatt ttacgtctcc tgccgccaga actggcgaca ctagggataa cggaactgga
     2401 gtatgagttt taccgtcggc tggtggaaaa acagttgctc acctatcgcc tgcacggtga
     2461 gtcgtggcgt gaaaaagtgc tcgaacgtcc ggtggtacat aaagattacg atgaacagcc
     2521 gcgcgggccg tttattgtct gtgtggatac ttccggctca atgggcggct ttaatgaaca
     2581 gtgtgcgaaa gcgttctgcc tggctttgat gcgcattgct ctcgctgaaa accggcgctg
     2641 ttatattatg ctattttcca ccgagatcgt ccgttatgag ctttcaggcc cacaaggcat
     2701 cgaacaagca atccgttttt taagccagca gtttcgtggc ggcaccgatc ttgccagttg
     2761 ttttcgcgcc attatggaac gcttgcaaag cagggaatgg tttgatgccg atgcggtggt
     2821 gatttctgat tttatcgctc agcggttgcc tgacgacgtg acgagtaaag tgaaagagct
     2881 gcagcgggta catcagcatc gctttcatgc cgtggcgatg tcggcacacg gcaaacccgg
     2941 catcatgcgc attttcgatc atatctggcg ctttgatacc gggatgcgaa gccgcctgct
     3001 cagacgctgg cggcgataaa ttattacagc agagaaggga cgctctcgcg aacagcagct
     3061 ggccatactc cacactgaac ctggccgata tgcggcagtt gcagcagcag catagtcaaa
     3121 cgagactggc cgataccgcc gccgatggtc tgcggcattt caccgcgcag cagcgcctga
     3181 tgccattcca gctgcagacg atcttcatca ccggtcagcg ccagctgatg cttcagcgtg
     3241 tcggcatcca cgcggatccc catggaagaa agctcaaacg catcttccag taccgggttc
     3301 cacaccagaa tatcgccgtt caggcccgca tggcccagct ctgacggggt gctccagtca
     3361 tcataatccg gtgcgcgcac gtcgtgacga tgaccatcac tcaacttgcc gccaatcccg
     3421 acgaggaata ccgcgccaag atctttcgca atcgcccgct cacgcccttt ggcatcgaga
     3481 tccggataac gagacagtaa ctcctggctg tgtacgaagt gaatctgatc cggcaggaac
     3541 ggtgccaggc caaactcttc gttaaccgca gcttcggttg ctttaattcc cgcccagatc
     3601 gcctctaccg tgcttttcag agtcgagagt tgacgctcac cgtcgcccat tacgcgttcc
     3661 cagtcccact ggtcaacata gaccgagtgc aacggagaaa gacggtcttc atcggggcga
     3721 agggctttca tgtgcgtgta cagcccttcg cccgcgctga agtcgtgttg ccctaaggtc
     3781 tgacgtttcc acttcgccag tgaatgaacc acttcgaact gggcatcagg cagagctttc
     3841 acttttacct gcaccgcttt ttcacagccc gacaagttat cctgcgtgcc atcccccaca
     3901 cggctaagaa tcggcgcctg gacttcgatc agccccagac gttcttccag ttggcgagaa
     3961 aagtgagatt tcacgaagct aatttggcgt tgtttggcaa tgtaagcggt tttcattttt
     4021 tatactcctg cgtcctgttg cttatgatta agcaacaaaa accgacacac atgcaataat
     4081 cattcaataa aaagcctaac acacttttga ttcattaaaa aagacggcta aaatagaatg
     4141 aatcatcaat ctgcataaga aaatcctatg gaaaattatc tgatcgacaa tctggaccgt
     4201 ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt acgccgaact ggcgaaacaa
     4261 tttggcgtca gtccggggac gattcacgtt cgagtagaga aaatgaagca ggcggggatc
     4321 attaccgggg cgcgtattga tgtcagcccg aagcagctcg gttatgacgt aggctgcttt
     4381 atcggcatta tattaaagag cgccaaagac tacccttccg cgctggcaaa gctggaaagc
     4441 cttgatgaag tcactgaagc ctattacaca accggccact acagcatctt tataaaagtt
     4501 atgtgccgtt cgatcgacgc tctccagcat gtacttatca acaagatcca aacaattgat
     4561 gaaattcagt ccaccgagac attgatcgtc ctgcaaaacc cgatcatgcg taccatcaag
     4621 ccctgatcgg cttttttaat cccatacttt tccacaggta gatcccaacg cgttcacagc
     4681 gtacaatacg ccactcttaa taaaggtggc ggtttatggc agatatcact cttatcagcg
     4741 gcagcaccct cggcggtgcc gaatatgtag cagaacacct ggctgaaaag ctggaagagg
     4801 tgggttttac caccgaaacg ctgcacggtc cgctgttgga agatttatct gcctcgggga
     4861 tctggctggt tatcagctcc acccacggtg ccggagatat tccggacaac ctttctcctt
     4921 tctatgaagc attgcaggaa cagaagcccg atctttctgc ggtccgcttt ggcgcaatcg
     4981 gtattggcag tcgtgaatat gacacctttt gtggggctat cgataaactc gaggcagaac
     5041 tcaaaaattc cggtgcaaaa cagacaggcg aaacactgaa gatcaacatt cttgatcacg
     5101 acattccgga agatccggca gaagaatggc tgggatcgtg gattaattta ctcaaataag
     5161 tatacagatc gtgcgatcta ctgtggataa ctctgtcagg aagcttggat caaccggtag
     5221 ctatccaaag aacaaccgtt gttcagtttt tgagttgtgt ataacccctc attctgatcc
     5281 cagcttatac ggtccaggat caccgatcat tcacagttaa tgatcctttc caggttgttg
     5341 atcttaaaaa tcggatcctt gttatccaca gggcagtgcg atcctaataa gagatcacaa
     5401 tagaacagat ctctaaataa atagatcttc tttttaatac ccaggatccc aggtctttct
     5461 caagccgaca aagttgagta gaatccacgg cccgggcttc aatccatttt cataccgcgt
     5521 tatgcgaggc aatcaccatg ttttatccgg atccttttga cgtcatcatc attggcgggg
     5581 gtcatgcagg caccgaggcc gcgatggctg cggcgcgtat gggtcaacag actctgcttt
     5641 tgacacacaa tatcgacact ctggggcaga tgagctgcaa cccggcgatc ggcggtattg
     5701 ggaagggaca tctggtaaaa gaagtggatg cactcggcgg tctgatggcg aaagcgatcg
     5761 atcaagcggg tatccagttt aggatactaa acgcaagcaa aggaccggcg gttcgcgcta
     5821 cccgagctca ggcggatcgt gtgcgctacc gtcaggcggt acgtacggcg ctggagaacc
     5881 aaccgaacct gatgatcttc cagcaggcgg ttgaagatct tattgtcgaa aacgatcgcg
     5941 tggtcggtgc tgttacccaa atgggactga agttccgtgc caaagctgtg gtgctcaccg
     6001 ttgggacgtt cctcgacggc aaaattcata tcggtctgga taattacagc ggtggccgtg
     6061 ctggcgatcc gccatccatt ccgctctctc gccgtttgcg tgaactgccg ctgcgcgttg
     6121 gtcgtctgaa aaccggtact ccgccgcgta ttgatgctcg aaccatcgac tttagcgtgc
     6181 tggcgcaaca gcatggcgat aacccaatgc cggtattctc gtttatgggc aatgcgtccc
     6241 tgcatcccca gcaggtgccg tgttatatca ctcataccaa cgagaaaacc catgatgtga
     6301 tccgcagtaa cctcgatcgt agcccaatgt atgcaggggt gatcgaaggt gtcggcccac
     6361 gctactgccc gtcgatcgaa gacaaagtca tgcgcttcgc agacagaaat cagcatcaga
     6421 tcttccttga accggaagga ctgacctcta atgaaattta tccgaacggt atctccacca
     6481 gcctgccgtt cgatgtgcag atgcaaatcg tccgctctat gcaggggatg gaaaacgcga
     6541 agatcgtgcg tccgggttat gccattgagt atgacttctt cgatcctcgc gacctgaaac
     6601 cgacgctgga gagcaagttt atccaggggc tgttctttgc tggtcagatt aacggcacta
     6661 ccggttacga agaagccgct gcgcaaggtt tgctggccgg tcttaacgct gcccgtctgt
     6721 ctgctgacaa agaaggttgg gctccggcac gttctcaggc gtatctcggc gtactggttg
     6781 atgacctgtg cactttagga accaaagaac cgtatcgtat gtttacttcg cgcgcagaat
     6841 atcgtctgat gctgcgcgaa gataatgcgg atctgcgttt gactgaaatc ggtcgtgaac
     6901 tgggcctggt ggatgacgaa cgttgggcgc gctttaacga gaaacttgag aatatcgagc
     6961 gtgagcgtca gcgtctgaaa tcgacctggg taaccccgtc ggcggaagct gcagccgaag
     7021 tgaatgctca cctgactgcg ccgctttccc gtgaagccag tggtgaagat ctgctgcgtc
     7081 gtccggaaat gacttatgaa aaattaacca cgctgacgcc gtttgcccct gcgttgacag
     7141 acgaacaggc ggcggaacag gttgagattc aggttaaata cgaaggttat atcgcgcgcc
     7201 agcaagatga gatcgaaaag cagctgcgta acgagaacac cctgctaccc gcgacactgg
     7261 attaccgcca gatatccggt ctttctaacg aagtgatcgc caaacttaac gatcacaaac
     7321 cggcctctat cggccaggct tcgcgtattt ctggcgtcac gcctgcggcc atctccattc
     7381 tgctggtgtg gctgaaaaaa cagggtatgc tgcgtcgtag cgcataacgc attaaaaatg
     7441 cctggtaagc acccgcttac caggcaacgc atcaagaaca ggtaatcacc gtgctcaaca
     7501 aactctcctt actgctgaaa gacgcaggta tttcgcttac tgatcaccag aaaaaccagc
     7561 ttattgccta cgtgaatatg ctgcataaat ggaacaaagc gtacaacctg acttcggtcc
     7621 gcgatcctaa tgagatgctg gtacgccata ttctcgatag cattgtggtg gcaccgtatc
     7681 tgcaaggtga acggtttatc gatgtcggca ccggaccagg actgccaggc attccactct
     7741 ctatcgtgcg tcctgaagcc catttcactc tgttggatag ccttggtaaa cgcgtgcgtt
     7801 tccttcgtca ggtgcaacat gagcttaaac tggagaatat tgaaccagta cagagcaggg
     7861 tagaagagtt tccttcagag ccgccatttg atggcgtaat tagccgcgct tttgcctctc
     7921 tgaacgatat ggtgagctgg tgccatcatc ttcctggtga gcaaggccgt ttctacgcgc
     7981 tgaaagggca aatgccggaa gatgaaatcg ctttgttgcc cgaagaatat caggtcgaat
     8041 cagtggttaa acttcaggtt ccagccctgg atggcgaacg tcatctggtg gtgattaaag
     8101 caaataaaat ttaattttta tcaaaaaaat cataaaaaat tgaccggtta gactgttaac
     8161 aacaaccaag ttttctactg atataactgg ttacatttaa cgccacgttc actcttttgc
     8221 atcaacaaga taacgtggct ttttttggta agcagaaaat aagtcattag tgaaaatatc
     8281 agtctgctaa aaatcggcgc taagaaccat cattggctgt taaaacatta ttaaaaatgt
     8341 caatgggtgg tttttgttgt gtaaatgtta tttattaaaa cagtatctgt ttttagactg
     8401 aaatatcata aacttgtaaa ggcatcattt gccaagtaaa taaatatgct gtgcgcgaac
     8461 atgcgcaata tgtgatctga agcacgcttt atcaccagtg tttacgcgtt atttacagtt
     8521 tttcatgatc gaacaggggt agcggaaaag tcgcaattgt atgcactgga aaaatattta
     8581 aacatttatt caccttttgg ctacttattg tttgaaatca cgggggcgca ccgtataatt
     8641 tgaccgcttt ttgatgcttg actctaagcc ttaaagaaag ttttatacga cacgcggcat
     8701 acctcgaagg gagcaggagt gaaaaacgtg atgtctgtgt cgctcgtgag tcgaaacgtt
     8761 gctcggaagc ttctgctcgt tcagttactg gtggtgatag caagtggatt gctgttcagc
     8821 ctcaaagacc ccttctgggg cgtctctgca ataagcgggg gcctggcagt ctttctgcct
     8881 aacgttttgt ttatgatatt tgcctggcgt caccaggcgc atacaccagc gaaaggccgg
     8941 gtggcttgga cattcgcatt tggcgaagct ttcaaagttc tggcgatgtt ggtgttactg
     9001 gtggtggcgt tggcggtttt aaaggcggta ttcttgccgc tgatcgttac gtgggttttg
     9061 gtgctggtgg ttcagatact ggcaccggct gtaattaaca acaaagggta aaaggcatca
     9121 tggcttcaga aaatatgacg ccgcaggatt acataggaca ccacctgaat aaccttcagc
     9181 tggacctgcg tacattctcg ctggtggatc cacaaaaccc cccagccacc ttctggacaa
     9241 tcaatattga ctccatgttc ttctcggtgg tgctgggtct gttgttcctg gttttattcc
     9301 gtagcgtagc caaaaaggcg accagcggtg tgccaggtaa gtttcagacc gcgattgagc
     9361 tggtgatcgg ctttgttaat ggtagcgtga aagacatgta ccatggcaaa agcaagctga
     9421 ttgctccgct ggccctgacg atcttcgtct gggtattcct gatgaatctg atggatttac
     9481 tgcctatcga cttgctgccg tacattgctg aacatgtact gggtctgcct gcactgcgtg
     9541 tggttccgtc tgcggacgtg aacgtaacgc tgtctatggc actgggcgta tttatcctga
     9601 ttctgttcta caacatcaaa atgaaaggca tcggcggctt cacgaaagag ttgacgctgc
     9661 agccgttcaa tcactgggcg ttcattcctg tcaacttaat ccttgaaggg gtaagcctgc
     9721 tgtccaaacc agtttcactc ggtttgcgac tgttcggtaa catgtatgcc ggtgagctga
     9781 ttttcattct gattgctggt ctgttgccgt ggtggtcaca gtggatcctg aatgtgccgt
     9841 gggccatttt ccacatcctg atcattacgc tgcaagcctt catcttcatg gttctgacga
     9901 tcgtctatct gtcgatggcg tctgaagaac attaatttac caacactact acgttttaac
     9961 tgaaacaaac tggagactgt catggaaaac ctgaatatgg atctgctgta catggctgcc
    10021 gctgtgatga tgggtctggc ggcaatcggt gctgcgatcg gtatcggcat cctcgggggt
    10081 aaattcctgg aaggcgcagc gcgtcaacct gatctgattc ctctgctgcg tactcagttc
    10141 tttatcgtta tgggtctggt ggatgctatc ccgatgatcg ctgtaggtct gggtctgtac
    10201 gtgatgttcg ctgtcgcgta gtaagcgttg cttttattta aagagcaata tcagaacgtt
    10261 aactaaatag aggcattgtg ctgtgaatct taacgcaaca atcctcggcc aggccatcgc
    10321 gtttgtcctg ttcgttctgt tctgcatgaa gtacgtatgg ccgccattaa tggcagccat
    10381 cgaaaaacgt caaaaagaaa ttgctgacgg ccttgcttcc gcagaacgag cacataagga
    10441 ccttgacctt gcaaaggcca gcgcgaccga ccagctgaaa aaagcgaaag cggaagccca
    10501 ggtaatcatc gagcaggcga acaaacgccg ctcgcagatt ctggacgaag cgaaagctga
    10561 ggcagaacag gaacgtacta aaatcgtggc ccaggcgcag gcagaaattg aagccgagcg
    10621 taaacgtgcc cgtgaagagc tgcgtaagca agttgctatc ctggctgttg ctggcgccga
    10681 gaagatcatc gaacgttccg tggatgaagc tgctaacagc gacatcgtgg ataaacttgt
    10741 cgctgaactg taaggaggga ggggctgatg tctgaattta ttacggtagc tcgcccctac
    10801 gccaaagcag cttttgactt tgccgtcgaa caccaaagtg tagaacgctg gcaggacatg
    10861 ctgacgtttg ccgccgaggt aaccaaaaac gaacaaatgg cagagcttct ctctggcgcg
    10921 cttgcgccag aaacgctcgc cgagtcgttt atcgcagttt gtggtgagca actggacgaa
    10981 aacggtcaga acctgattcg ggtaatggct gaaaatggtc gtcttaacgc gctcccggat
    11041 gttctggagc agtttattca ccttcgtgcc gtgagtgagg ctaccgctga ggtagacgtc
    11101 atttccgctg ccgcactgag tgaacaacag ctcgcgaaaa tttctgctgc gatggaaaaa
    11161 cgtctgtcac gcaaagttaa gctgaattgc aaaatcgata agtctgtaat ggcaggcgtt
    11221 atcatccgag cgggtgatat ggtcattgat ggcagcgtac gcggtcgtct tgagcgcctt
    11281 gcagacgtct tgcagtctta aggggactgg agcatgcaac tgaattccac cgaaatcagc
    11341 gaactgatca agcagcgcat tgctcagttc aatgttgtga gtgaagctca caacgaaggt
    11401 actattgttt ctgtaagtga cggtgttatc cgcattcacg gcctggccga ttgtatgcag
    11461 ggtgaaatga tctccctgcc gggtaaccgt tacgctatcg cactgaacct cgagcgcgac
    11521 tctgtaggtg cggttgttat gggtccgtac gctgaccttg ccgaaggcat gaaagttaag
    11581 tgtactggcc gtatcctgga agttccggtt ggccgtggcc tgctgggccg tgtggttaac
    11641 actctgggtg caccaatcga cggtaaaggt ccgctggatc acgacggctt ctctgctgta
    11701 gaagcaatcg ctccgggcgt tatcgaacgt cagtccgtag atcagccggt acagaccggt
    11761 tataaagccg ttgactccat gatcccaatc ggtcgtggtc agcgtgaatt gatcatcggt
    11821 gaccgtcaga caggtaaaac cgcactggct atcgatgcca tcatcaacca gcgcgattcc
    11881 ggtatcaaat gtatctatgt cgctatcggc cagaaagcgt ccaccatttc taacgtggta
    11941 cgtaaactgg aagagcacgg cgcactggct aacaccatcg ttgtggtagc aaccgcgtct
    12001 gaatccgctg cactgcaata cctggcaccg tatgccggtt gcgcaatggg cgaatacttc
    12061 cgtgaccgcg gtgaagatgc gctgatcatt tacgatgacc tgtctaaaca ggctgttgct
    12121 taccgtcaga tctccctgct gctccgtcgt ccgccaggac gtgaagcatt cccgggcgac
    12181 gttttctacc tccactctcg tctgctggag cgtgctgcac gtgttaacgc cgaatacgtt
    12241 gaagccttca ccaaaggtga agtgaaaggg aaaaccggtt ctctgaccgc actgccgatt
    12301 atcgaaactc aggcgggtga cgtttctgcg ttcgttccga ccaacgtaat ctccattacc
    12361 gatggtcaga tcttcctgga aaccaacctg ttcaacgccg gtattcgtcc tgcggttaac
    12421 ccgggtattt ccgtatcccg tgttggtggt gcagcacaga ccaagatcat gaaaaaactg
    12481 tccggtggta tccgtaccgc tctggcacag tatcgtgaac tggcagcgtt ctctcagttt
    12541 gcatccgacc ttgacgatgc aacacgtaag cagcttgacc acggtcagaa agtgaccgaa
    12601 ctgctgaaac agaaacagta tgcgccgatg tccgttgcgc agcagtctct ggttctgttc
    12661 gcagcagaac gtggttacct ggcggatgtt gaactgtcga aaattggcag cttcgaagcc
    12721 gctctgctgg cttacgtcga ccgtgatcac gctccgttga tgcaagagat caaccagacc
    12781 ggtggctaca acgacgaaat cgaaggcaag ctgaaaggca tcctcgattc cttcaaagca
    12841 acccaatcct ggtaacgtct ggcggcttgc cttagggcag gccgcaaggc attgaggaga
    12901 agctcatggc cggcgcaaaa gagatacgta gtaagatcgc aagcgtccag aacacgcaaa
    12961 agatcactaa agcgatggag atggtcgccg cttccaaaat gcgtaaatcg caggatcgca
    13021 tggcggccag ccgtccttat gcagaaacca tgcgcaaagt gattggtcac cttgcacacg
    13081 gtaatctgga atataagcac ccttacctgg aagaccgcga cgttaaacgc gtgggctacc
    13141 tggtggtgtc gaccgaccgt ggtttgtgcg gtggtttgaa cattaacctg ttcaaaaaac
    13201 tgctggcgga aatgaagacc tggaccgaca aaggcgttca atgcgacctc gcaatgatcg
    13261 gctcgaaagg cgtgtcgttc ttcaactccg tgggcggcaa tgttgttgcc caggtcaccg
    13321 gcatggggga taacccttcc ctgtccgaac tgatcggtcc ggtaaaagtg atgttgcagg
    13381 cctacgacga aggccgtctg gacaagcttt acattgtcag caacaaattt attaacacca
    13441 tgtctcaggt tccgaccatc agccagctgc tgccgttacc ggcatcagat gatgatgatc
    13501 tgaaacataa atcctgggat tacctgtacg aacccgatcc gaaggcgttg ctggataccc
    13561 tgctgcgtcg ttatgtcgaa tctcaggttt atcagggcgt ggttgaaaac ctggccagcg
    13621 agcaggccgc ccgtatggtg gcgatgaaag ccgcgaccga caatggcggc agcctgatta
    13681 aagagctgca gttggtatac aacaaagctc gtcaggccag cattactcag gaactcaccg
    13741 agatcgtctc gggggccgcc gcggtttaaa caggttattt cgtagaggat ttaagatggc
    13801 tactggaaag attgtccagg taatcggcgc tgtagttgac gtcgaattcc ctcaggatgc
    13861 cgtaccgcgc gtgtacgatg ctcttgaggt gcaaaatggt aatgagcgtc tggtgctgga
    13921 agttcagcag cagctcggcg gcggtatcgt gcgtaccatc gcaatgggtt cctccgacgg
    13981 tctgcgtcgc ggtctggatg taaaagacct cgaacacccg atcgaagtcc cggtaggtaa
    14041 agcgactctg ggccgtatca tgaacgtact gggtgaaccg gtcgacatga aaggcgagat
    14101 cggtgaagaa gagcgttggg cgattcaccg cgcagcacct tcctacgaag agctgtcaaa
    14161 ctctcaggaa ctgctggaaa ccggtatcaa agttatcgac ctgatgtgtc cgttcgctaa
    14221 gggcggtaaa gttggtctgt tcggtggtgc gggtgtaggt aaaaccgtaa acatgatgga
    14281 gctcattcgt aacatcgcga tcgagcactc cggttactct gtgtttgcgg gcgtaggtga
    14341 acgtactcgt gagggtaacg acttctacca cgaaatgacc gactccaacg ttatcgacaa
    14401 agtatccctg gtgtatggcc agatgaacga gccgccggga aaccgtctgc gcgttgctct
    14461 gaccggtctg accatggctg agaaattccg tgacgaaggt cgtgacgttc tgctgttcgt
    14521 tgacaacatc tatcgttaca ccctggccgg tacggaagta tccgcactgc tgggccgtat
    14581 gccttcagcg gtaggttatc agccgaccct ggcggaagag atgggcgttc tgcaggaacg
    14641 tatcacctcc accaaaactg gttctatcac ctccgtacag gcggtatacg tacctgcgga
    14701 tgacttgact gacccgtctc cggcaaccac ctttgcgcac cttgacgcaa ccgtggtact
    14761 gagccgtcag atcgcgtctc tgggtatcta cccggccgtt gacccgctgg actccaccag
    14821 ccgtcagctg gacccgctgg tggttggtca ggaacactac gacaccgcgc gtggcgttca
    14881 gtccatcctg caacgttatc aggaactgaa agacatcatc gccatcctgg gtatggatga
    14941 actgtctgaa gaagacaaac tggtggtagc gcgtgctcgt aagatccagc gcttcctgtc
    15001 ccagccgttc ttcgtggcag aagtattcac cggttctccg ggtaaatacg tctccctgaa
    15061 agacaccatc cgtggcttta aaggcatcat ggaaggcgaa tacgatcacc tgccggagca
    15121 ggcgttctac atggtcggtt ccatcgaaga agctgtggaa aaagccaaaa aactttaacg
    15181 ccttaatcgg agggtgatat ggcaatgact taccacctgg acgtcgtcag cgcagagcaa
    15241 caaatgttct ctggtctggt cgagaaaatc caggtaacgg gtagcgaagg tgaactgggg
    15301 atctaccctg gccacgcacc gctgctcacc gccattaagc ctggtatgat tcgcatcgtg
    15361 aaacagcacg gtcacgaaga gtttatctat ctgtctggcg gcattcttga agtgcagcct
    15421 ggcaacgtga ccgttctggc cgacaccgca attcgcggcc aggatctcga cgaagcgcga
    15481 gccatggaag cgaaacgtaa ggctgaagag cacattagca gctctcacgg cgacgtagat
    15541 tacgctcagg cgtctgcgga actggccaaa gcgatcgcgc agctgcgcgt tatcgagttg
    15601 accaaaaaag cgatgtaaca ccggcttgaa aagcacaaaa gccagtctgg aaacaggctg
    15661 gcttttttta cgcgttgacc cgtcctgaag agcgttcaca tagatcctgc tgatataaaa
    15721 cacccctgtt ttcccgttta ttcattgatc gaaataagag caaaaacatc cacctgacgc
    15781 ttaaatgaag gtactgcctt aattttctgc agacaaaagg cgtgacgatg gtcgaaaatg
    15841 gcactttcgt cagcggggat aatccgttat tgaacaattt atcctctgtc catttcacga
    15901 tgaaaaaaat gtagtttttt caaggtgaag cggtttaaat tcgttctcaa attacagtca
    15961 ggacgcgtat gttgaataat gctatgagcg tagtgatcct tgccgcaggc aaaggcacgc
    16021 gcatgtattc cgatcttccg aaagtgctgc atacccttgc cgggaaagcg atggttcagc
    16081 atgtcattga tgctgcgaat gaattaggcg cagcgcacgt tcacctggtg tacggtcacg
    16141 gcggcgatct gcttaaacag gcgctgaaag acgacaacct taactgggtg cttcaggcag
    16201 agcagctggg tacgggtcat gcaatgcagc aggccgcacc tttctttgcc gatgatgaag
    16261 acattttaat gctctacggc gacgtgccgc tgatctctgt cgaaacactc cagcgcctgc
    16321 gtgatgctaa accccagggt ggcattggtc tgctgacggt gaaactggat gatccgaccg
    16381 gttatggacg tatcacccgt gaaaatggca aagttaccgg cattgttgag cacaaagatg
    16441 ccaccgacga gcagcgtcag attcaggaga tcaacaccgg cattctgatt gccaacggcg
    16501 cagatatgaa acgctggctg gcgaagctga ccaacaataa tgctcagggc gaatactaca
    16561 tcaccgacat tattgcgctg gcgtatcagg aagggcgtga aatcgtcgcc gttcatccgc
    16621 aacgtttaag cgaagtagaa ggtgtgaata accgcctgca actctcccgt ctggagcgcg
    16681 tttaccagtc cgaacaggct gaaaaactgc tgttagcagg cgttatgctg cgcgatccgg
    16741 cgcgttttga tctgcgcggt acgcttactc acgggcgcga tgttgaaatt gatactaacg
    16801 ttatcatcga gggcaacgtg actctcggtc atcgcgtgaa aatcggcacc ggttgcgtga
    16861 ttaaaaacag cgtgattggc gatgattgcg aaatcagtcc gtataccgtt gtggaagacg
    16921 cgaatctggc agcggcctgt actattggcc cgtttgcccg tctgcgtcct ggtgctgagt
    16981 tgctggaagg tgcacacgtt ggtaacttcg ttgagatgaa aaaagcgcgt ctgggtaaag
    17041 gcacgaaagc cgggcatctg acttacctgg gcgatgcgga aattggcgat aacgttaaca
    17101 tcggcgcggg aaccattacc tgcaactacg atggtgcgaa taaatttaag accattatcg
    17161 gcgacgatgt gtttgtcggt tccgacactc agctggtggc cccggtaaca gtaggcaaag
    17221 gcgcgaccat tgctgcgggt acaactgtga cgcgtaatgt cggcgaaaat gcattagcta
    17281 tcagccgtgt gccgcagact cagaaagaag gctggcgtcg tccggtaaag aaaaagtaat
    17341 tctggccggg taatccggtc atctgggatg aggagataac ataatctccc tcccacaagc
    17401 agtaactata aaaataaccc cactctctac aaggctcggg gcgcccgaaa aaacgggcat
    17461 acaggttgac cgacaacgat ataaatcgga atcaaaaact atgtgtggaa ttgttggcgc
    17521 gatcgcgcaa cgtgatgtag cagaaatcct tcttgaaggt ttacgtcgtc tggaataccg
    17581 cggatatgac tctgccggtc tggccgttgt tgatgcggaa ggtcatatga ctcgcctgcg
    17641 tcgcctcggt aaagtccaga tgctggctca ggcagcggaa gaacatcctc tgcatggcgg
    17701 caccggtatt gctcacactc gctgggcgac ccacggtgaa ccttcagaag ctaacgcgca
    17761 tccgcatgtt tctgaacaca ttgtggtggt gcataatggc atcatcgaaa accatgaacc
    17821 gctgcgtgaa gagttaaaag cgcgtggcta taccttcgtt tctgaaaccg acaccgaagt
    17881 gattgcccat ctggtgaact gggagctgaa acaaggcggg actctgcgtg aggctgttct
    17941 gcgtgctatc ccgcagctgc gcggtgcgta cggtacagtg atcatggact cccgtcaccc
    18001 ggataccctg ctggcggcac gttctggtag tccgctggtg attggcctgg gtatgggcga
    18061 aaactttatc gcttctgacc agctggcgct gttgccggtg acccgtcgct ttatcttcct
    18121 tgaagagggc gatattgcgg aaatcactcg ccgttcggta aacatcttcg ataaaactgg
    18181 cgcggaagta aaacgtcagg atatcgaatc caatctgcaa tatgacgcgg gcgataaagg
    18241 tatttaccgt cactacatgc agaaagagat ctacgaacag ccgaacgcga taaaaaacac
    18301 ccttaccggg cgcatcagcc acggtcaggt tgatttaagc gagctgggac cgaacgccga
    18361 cgaactgctg tcgaaggttg agcatattca gatcctcgcc tgtggtactt cttataactc
    18421 cggtatggtt tcccgctact ggtttgaatc gctggcaggt attccgtgcg acgtcgaaat
    18481 cgcctctgaa ttccgctatc gtaaatctgc cgtgcgtcgt aacagcctga tgatcacctt
    18541 gtcacagtct ggcgaaaccg cggataccct ggcgggcctg cgtctgtcga aagagctggg
    18601 ttaccttggt tcgctagcaa tctgtaacgt tccgggttct tctctggtgc gcgaatccga
    18661 tctggcgctg atgaccaacg cgggtacgga aatcggcgtg gcgtccacta aagcattcac
    18721 cactcagtta actgtgctgt tgatgctggt ggcgaagctg tctcgcctga aaggtctgga
    18781 tgcctccatt gaacatgaca ttgtgcatgg tctgcaggcg ctgccgagcc gtattgagca
    18841 gatgctgtct caggataaac gcattgaagc tctggcagaa gatttctctg acaaacatca
    18901 cgcgctgttc ctgggccgtg gcgatcagta cccaatcgcg ctggaaggcg cattgaagct
    18961 gaaagagatc tcttacattc acgctgaagc ctacgctgca ggcgagctga aacacggccc
    19021 gctggcgctg attgacgcgg atatgccggt tatcgtcgtt gcaccgaaca acgaactgtt
    19081 ggaaaaactg aaatccaaca ttgaagaagt tcgcgcgcgt ggcggtcaat tgtatgtctt
    19141 cgccgatcag gatgcgggtt ttgtaagtag cgataacatg cacatcatcg agatgccgca
    19201 tgtggaagag gtgattgcac cgatcttcta caccgttccg ctgcagctgc tggcttacca
    19261 tgtcgcgctg atcaaaggca ccgacgttga ccagccgcgt aacctggcaa aatcggttac
    19321 ggttgagtaa tcactatcat agataaaaaa gggcttgtgg gcggacaata aagtcttaaa
    19381 ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa
    19441 actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc
    19501 aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg
    19561 gcatggtaaa gactatattc catggctaac agtacaagaa gttccttctt caggtcgttc
    19621 ccaccgtatt tattctcata agacgggacg agtccatcat ttgctatctg acttagagct
    19681 tgctgttttt ctcagtcttg agtgggagag cagcgtgcta gatatacgcg agcagttccc
    19741 cttattacct agtgatacca ggcagattgc aatagatagt ggtattaagc atcctgttat
    19801 tcgtggtgta gatcaggtta tgtctactga ttttttagtg gactgcaaag atggtccttt
    19861 tgagcagttt gctattcaag tcaaacctgc agcagcctta caagacgagc gtaccttaga
    19921 aaaactagaa ctagagcgtc gctattggca gcaaaagcaa attccttggt tcatttttac
    19981 tgataaagaa ataaatcccg tagtaaaaga aaatattgaa tggctttatt cagtgaaaac
    20041 agaagaagtt tctgcggagc ttttagcaca actatcccca ttggcccata tcctgcaaga
    20101 aaaaggagat gaaaacatta tcaatgtctg taagcaggtt gatattgctt atgatttgga
    20161 gttaggcaaa acattgagtg agatacgagc cttaaccgca aatggtttta ttaagttcaa
    20221 tatttataag tctttcaggg caaataagtg tgcagatctc tgtattagcc aagtagtgaa
    20281 tatggaggag ttgcgctatg tggcaaatta atgaggttgt gctatttgat aatgatccgt
    20341 atcgcatttt ggctatagag gatggccaag ttgtctggat gcaaataagc gctgataaag
    20401 gagttccaca agctagggct gagttgttgc taatgcagta tttagatgaa ggccgcttag
    20461 ttagaactga tgacccttat gtacatcttg atttagaaga gccgtctgta gattctgtca
    20521 gcttccagaa gcgcgaggag gattatcgaa aaattcttcc tattattaat agtaaggatc
    20581 gtttcgaccc taaagtcaga agcgaactcg ttgagcatgt ggtccaagaa cataaggtta
    20641 ctaaggctac agtttataag ttgttacgcc gttactggca gcgtggtcaa acgcctaatg
    20701 cattaattcc tgactacaaa aacagcggtg caccagggga aagacgttca gcgacaggaa
    20761 cagcaaagat tggccgagcc agagaatatg gtaagggtga aggaaccaag gtaacgcccg
    20821 agattgaacg cctttttagg ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa
    20881 agaccaccgt tgcctataga cgatttgtgg acttgtttgc tcagtatttt cctcgcattc
    20941 cccaagagga ttacccaaca ctacgtcagt ttcgttattt ttatgatcga gaatacccta
    21001 aagctcagcg cttaaagtct agagttaaag caggggtata taaaaaagac gtacgaccct
    21061 taagtagtac agccacttct caggcgttag gccctgggag tcgttatgag attgatgcca
    21121 cgattgctga tatttattta gtggatcatc atgatcgcca aaaaatcata ggaagaccaa
    21181 cgctttacat tgtgattgat gtgtttagtc ggatgatcac gggcttttat atcggctttg
    21241 aaaatccgtc ttatgtggtg gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg
    21301 ccatttgtgc ccagcatgat attgagatta gtagctcaga ctggccgtgt gtaggtttgc
    21361 cagatgtgtt gctagcggac cgtggcgaat taatgagtca tcaggtcgaa gccttagttt
    21421 ctagttttaa tgtgcgagtg gaaagtgctc cacctagacg tggcgatgct aaaggcatag
    21481 tggaaagcac ttttagaaca ctacaagccg agtttaagtc ctttgcacct ggcattgtag
    21541 agggcagtcg gatcaaaagc catggtgaaa cagactatag gttagatgca tctctgtcgg
    21601 tatttgagtt cacacaaatt attttgcgta cgatcttatt cagaaataac catctggtga
    21661 tggataaata cgatcgagat gctgattttc ctacagattt accgtctatt cctgtccagc
    21721 tatggcaatg gggtatgcag catcgtacag gtagtttaag ggctgtggag caagagcagt
    21781 tgcgagtagc gttactgcct cgccgaaagg tctctatttc ttcatttggc gttaatttgt
    21841 ggggtttgta ttactcgggg tcagagattc tgcgtgaggg ttggttgcag cggagcactg
    21901 atatagctag acctcaacat ttagaagcgg cttatgaccc agtgctggtt gatacgattt
    21961 atttgtttcc gcaagttggc agccgtgtat tttggcgctg taatctgacg gaacgtagtc
    22021 ggcagtttaa aggtctctca ttttgggagg tttgggatat acaagcacaa gaaaaacaca
    22081 ataaagccaa tgcgaagcag gatgagttaa ctaaacgcag ggagcttgag gcgtttattc
    22141 agcaaaccat tcagaaagcg aataagttaa cgcccagtac tactgagccc aaatcaacac
    22201 gcattaagca gattaaaact aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg
    22261 cggagcattt gaagccaagc tcttcaggtg atgaggctaa agttattcct ttcaacgcag
    22321 tggaagcgga tgatcaagaa gattacagcc tacccacata cgtgcctgaa ttatttcagg
    22381 atccaccaga aaaggatgag tcatgagtgc tacccggatt caagcagttt atcgtgatac
    22441 gggggtagag gcttatcgtg ataatccttt tatcgaggcc ttaccaccat tacaagagtc
    22501 agtgaatagt gctgcatcac tgaaatcctc tttacagctt acttcctctg acttgcaaaa
    22561 gtcccgtgtt atcagagctc ataccatttg tcgtattcca gatgactatt ttcagccatt
    22621 aggtacgcat ttgctactaa gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg
    22681 cagaaatcct aaaacaggag atttacaaaa gcatttacaa aatggttatg agcgtgttca
    22741 aacgggagag ttggagacat ttcgctttga ggaggcacga tctacggcac aaagcttatt
    22801 gttaattggt tgttctggta gtgggaagac gacctctctt catcgtattc tagccacgta
    22861 tcctcaggtg atttaccatc gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga
    22921 ctgctcgcat aatggttcgc taaaagaaat ctgcttgaat tttttcagag cgttggatcg
    22981 agccttgggc tcgaactatg agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat
    23041 gttggctttg atgtcgcaaa tagccaatgc acatgcttta gggttgttgg ttattgatga
    23101 aattcagcat ttaagccgct ctcgttcggg tggatctcaa gagatgctga acttttttgt
    23161 gacgatggtg aatattattg gcgtaccagt gatgttgatt ggtaccccta aagcacgaga
    23221 gatttttgag gctgatttgc ggtctgcacg tagaggggca gggtttggag ctatattctg
    23281 ggatcctata caacaaacgc aacgtggaaa gcccaatcaa gagtggatcg cttttacgga
    23341 taatctttgg caattacagc ttttacaacg caaaga
//
LOCUS       DAJTNW010000065        22457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNW010000065 DAJTNW010000000
VERSION     DAJTNW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..280)
                     /locus_tag="OO179_002652"
     CDS             complement(<1..280)
                     /locus_tag="OO179_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532659.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPS"
     gene            418..1338
                     /locus_tag="OO179_002653"
     CDS             418..1338
                     /locus_tag="OO179_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5532660.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1338..2273
                     /locus_tag="OO179_002654"
                     /pseudo
     CDS             1338..2273
                     /locus_tag="OO179_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2291..2929
                     /locus_tag="OO179_002655"
     CDS             2291..2929
                     /locus_tag="OO179_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5532661.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3220..3948
                     /gene="mpaA"
                     /locus_tag="OO179_002656"
     CDS             3220..3948
                     /gene="mpaA"
                     /locus_tag="OO179_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5532662.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3923..4882)
                     /gene="ycjG"
                     /locus_tag="OO179_002657"
     CDS             complement(3923..4882)
                     /gene="ycjG"
                     /locus_tag="OO179_002657"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5532663.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5001..5507
                     /gene="tpx"
                     /locus_tag="OO179_002658"
     CDS             5001..5507
                     /gene="tpx"
                     /locus_tag="OO179_002658"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5532664.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5551..7092)
                     /gene="tyrR"
                     /locus_tag="OO179_002659"
     CDS             complement(5551..7092)
                     /gene="tyrR"
                     /locus_tag="OO179_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5532665.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7240..8301)
                     /locus_tag="OO179_002660"
     CDS             complement(7240..8301)
                     /locus_tag="OO179_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5532666.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8298..9695)
                     /locus_tag="OO179_002661"
     CDS             complement(8298..9695)
                     /locus_tag="OO179_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5532667.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9851..10849
                     /locus_tag="OO179_002662"
     CDS             9851..10849
                     /locus_tag="OO179_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5532668.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(10960..11832)
                     /gene="ompG"
                     /locus_tag="OO179_002663"
                     /pseudo
     CDS             complement(10960..11832)
                     /gene="ompG"
                     /locus_tag="OO179_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(11905..12986)
                     /locus_tag="OO179_002664"
                     /pseudo
     CDS             complement(11905..12986)
                     /locus_tag="OO179_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13000..13659)
                     /gene="pgmB"
                     /locus_tag="OO179_002665"
     CDS             complement(13000..13659)
                     /gene="pgmB"
                     /locus_tag="OO179_002665"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5532669.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13656..15923)
                     /gene="ycjT"
                     /locus_tag="OO179_002666"
     CDS             complement(13656..15923)
                     /gene="ycjT"
                     /locus_tag="OO179_002666"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5532670.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15920..16543)
                     /locus_tag="OO179_002667"
     CDS             complement(15920..16543)
                     /locus_tag="OO179_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5532671.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16540..16962)
                     /locus_tag="OO179_002668"
     CDS             complement(16540..16962)
                     /locus_tag="OO179_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5532672.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(16984..17759)
                     /locus_tag="OO179_002669"
                     /pseudo
     CDS             complement(16984..17759)
                     /locus_tag="OO179_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17777..18829)
                     /locus_tag="OO179_002670"
     CDS             complement(17777..18829)
                     /locus_tag="OO179_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5532673.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18860..19702)
                     /locus_tag="OO179_002671"
     CDS             complement(18860..19702)
                     /locus_tag="OO179_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5532674.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19689..20570)
                     /locus_tag="OO179_002672"
     CDS             complement(19689..20570)
                     /locus_tag="OO179_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5532675.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20591..21874)
                     /locus_tag="OO179_002673"
                     /pseudo
     CDS             complement(20591..21874)
                     /locus_tag="OO179_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(21888..>22457)
                     /gene="ycjM"
                     /locus_tag="OO179_002674"
     CDS             complement(21888..>22457)
                     /gene="ycjM"
                     /locus_tag="OO179_002674"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5532676.1"
                     /translation="KNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAILLS
                     FPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVYHE
                     LSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVNIT
                     DLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5524 a         6052 c         5479 g         5402 t
ORIGIN      
        1 cggatgggcg gttctgcaga tcgctcaggg atgtcagggc agaatctatg tcatgcaata
       61 tagggccaag aacagacaaa agatgctcgc ccgcttcagt gggtacaacg ctgcgcgtgg
      121 ttcgcgtcag aagccgcaat cccaatcgtt cttctatacg gcgcactatc tggcttaaag
      181 ctgactgcac catgctcagg cgggctgctg cgcgagtgaa gctacgctcc tctgcaacga
      241 cgacaaacgc catcagatca gcgatttctt cacgcttcat tcagtatctc catacaatct
      301 attaatcatt taaatgatag gtctaatcat gatttagggt ctaatcaact taatgatcaa
      361 tatctatagt gatatcacca accgtcaaaa cgaaccgaca actccatgag gaactgaatg
      421 atgaataata aagtcagctt cactaacagc aataatccaa ccatctcttt gtctgcagtg
      481 atctatttcc ctccaaaatt tgatgaaacc cgtcagtatc cggcaattgt ggtatctcac
      541 ccaggtgggg gcgttaaaga gcaaacggcc ggaacctatg ccgaaaagct ggcggaaaag
      601 ggatttgtca ctattgctta tgacgcatct tatcaaggtg aaagtggcgg cgagccgcgt
      661 cagttagaaa acccatacat tcgtactgaa gacgtcagtg ccgtgattga ttacctgaca
      721 acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg gaatttgtgc cggtgcagga
      781 tatacagcca atgctgcaat tcaggatcgc cgcatcaagg ccatcggtac cgttagtgcg
      841 gtcaatattg gttcgatgtt ccgcaatggt tgggaaaaca atgtgaaatc tattgatgct
      901 ctgccacaca ttgaagctgg ctcaaacgcc cgaaccagcg atattagcag cgaagaatac
      961 gccattatgc cactggctcc aatgaaggag gccgatgcgc cgaacgaaga actgcgtcag
     1021 gcctgggagt attaccacac acctcgtgcg cagtatccaa cagcaccggg ttacgctact
     1081 ttgcgcagcc ttaaccagat tattacctat gatgcttacc atatggcgga agtgtacctg
     1141 actcagccga tgcagattgt ggcgggcagc caggcaggga gcaaatggat gagtgacgac
     1201 ctgtacgatc gggcatcaag ccaggataaa cgctatcaca ttgttgaagg cgcaaaccat
     1261 atggatcttt atgacggcaa agcctatgtg gctgaagcaa tttcagtatt agctccgttc
     1321 tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa tagtgatatg gcctggcata
     1381 ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc aaaacagtat ccggcgatta
     1441 tcagcgtcca tccttttggc agttgtaagg agcagacgtc tggcaatatc tatggcaagg
     1501 cattggctga aaaaggctat gtcgttctcg cttatgacgc cagtttccag ggagaatctg
     1561 gtgggtagcc tcgttggata gaagatccga cacagcgcgt agaggatatt agccgtgtta
     1621 tcgattatgc agtaactctg ccatatgttg atgctgaacg cattggcgtg ctgggtgtgt
     1681 gggggggggg gggtggctat gccattaatg ccgcgctgac agaaaaacga attaaagctg
     1741 tagtgagtat tacgggtgtt aatatcgggc gtctttttcg ggaaggtttc agcaattatg
     1801 atccgattgg cgtattgaat gcaatggcgt cacaacgagc aaaagaggct cgtggtggag
     1861 aattacagat caacgaatta cttcccgcca gcctggatgc agcaaaagcc catggactga
     1921 ccgaacgcga tgtttatgaa gcaactgact actataaaac accacgcggg caacaaccgg
     1981 gaggtgcgac aaaaatgttg ttctcacatg cacagaaaac gctggcctgg gatgccttcg
     2041 catttactga agtgttactg acccagccgg tgatggtagt agttggtgaa aaagtcggtg
     2101 ccttcggtgc ttatcgtgat ggactggaag tctatggccg ggcgatggtt tcgcaggatc
     2161 gtcagttagt ttcgttaccc gatttctcac attatgaact atatgacaaa ccggaagcag
     2221 ttcaggaggc gctggcaaaa gttattcctt ttttcaacac ccatctggga taagtttcat
     2281 gagaaatatt atgaaaaatg tactgattct tggtgctggc ggtcagatag cccgccatgt
     2341 gattaatcaa ctggcagata aacaaactat taaacaaact ttatttgccc gtcagccagc
     2401 aaaaatacac aaaccctatc caactaatag ccagattatt atgggggatg tactgaatca
     2461 tgcagcactg aagcaggcca tgcagggaca ggatattgtg tatgccaacc ttacggggga
     2521 agatttagat attcaggcaa attcagtgat cgctgcgatg aaagcctgtg atgttaaacg
     2581 tctgatcttt gttctttccc tcggtattta tgatgaagtt ccaggaaaat ttgtcgaatg
     2641 gaataatgca gttattggtg aacccctaaa accattccgc agggctgcgg atgcaattga
     2701 ggcttctgga ctggaatata caattcttcg ccctgcctgg ctgactgatg aagatattat
     2761 tgattatgag cttacctctc gcaatgaacc attcaaaggt acgatcgtat cacgtaagag
     2821 tgtcgcagca ttaattacag atatcatcga taaacctgag aaacacattg gtgagaatat
     2881 aggcatcaat caacccggga ctgatggcga taagccattc ttcatgtaaa tcaattatat
     2941 agataccatc gtactgtact atgtgcagta cgatttatta aacttattaa ggtgaactga
     3001 tcaatcagat gcgattaaca tgtgtagaac cagtaagaat aaaatacgcc atgtatataa
     3061 cattacgaca attctaatat ctgcacatca atgaaaatgc aaacaacttc cggcaaaaaa
     3121 gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt tgataatccg caaaatttgc
     3181 acaacactgc ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgctcacgc
     3241 gccgaacgcg gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg
     3301 ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc
     3361 actcacggtg atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gactttgaca
     3421 ccttctttgc gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg
     3481 ttacgggcca atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag
     3541 gaaggtgaaa cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg
     3601 acaggtgaca aacctggctc cgaacctgaa acccaggcgc tgtgccagct tatacaccgc
     3661 attcaacctg cctgggtggt ctcattccac gatccactgg cttgtattga agatcccaga
     3721 cacagcgaat taggcgaatg gctggcccag tcgtttgaat tgcctctggt aaccagcgtc
     3781 ggttatgaaa cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc
     3841 accgcagagt ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg
     3901 gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg
     3961 gttccacatc taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca
     4021 ccagcggtaa agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac
     4081 tgaaaccttg tgcacgcgct tcagtcgcca gcgcttccgt cagaccaccg gttttatcga
     4141 gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta cgagtatgac
     4201 aactttcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc gcatcgtcct
     4261 gcgcaggaag cggttgttca agcatcgcaa cgcctaaatc cgccaatagc tggcaacgcg
     4321 ccgccaaccc ttctgcacgc caggattcat ttgcatcaac gatcagcgtg gcatcgggca
     4381 cagcggtgcg aattgccacc atccgctcac tgataagatg gttatccagc ttcactttca
     4441 gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga tcaggcgtac
     4501 cgatgacaac cgtctgtgca gtgataactg tctcgggaag cgttatgccg atcaaatcag
     4561 ccagcgattg ctgctgtttt cgcgccgcga gatcccacaa tgcacaatcc agcgcattac
     4621 gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct ttctctaatt
     4681 gcggcacgac gctcataatt tgcgccatta ccgaggcatc actttctcca taacgcggat
     4741 acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc accaccacgc
     4801 gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc caggcttcct
     4861 caaatacctt aacgattctc atctttactc cttgttgtcg cggtggtctg cggattaagc
     4921 gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac gttaactata
     4981 tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac ccggttacag
     5041 tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc gtggcaaaag
     5101 atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg aacattttcc
     5161 cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa ctggcaactg
     5221 agatcgacaa caccgttgtg ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt
     5281 tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt aacgctgaat
     5341 ttctgcaagc ctacggtgtg gcaattgctg atggcccact gaaaggtctg gcagcgcgtg
     5401 ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg gatgaaatca
     5461 ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataagtt aatatgcctg
     5521 atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct gactcagacc
     5581 atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt ttgccagttt
     5641 gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa aacggctggt
     5701 gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg cggcgtcata
     5761 atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg
     5821 atagatagcg ttctttaact gccgcacatt tcccggccac gcataacgtg taagtacagt
     5881 attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac
     5941 gaacagctca gttaacggca taatgtcctg cggacagtca cgtagcggcg gcagattgag
     6001 cgtcaacacg ttcagacgat aatagagatc ttcacggaac acgccttttt gcaccagttc
     6061 gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct catggtcttc
     6121 gccaacccga cggaaagtgc catcattaag aaaacgcagt aatttcgcct gcatccgtgg
     6181 tgacatttcc cctatttcat ccaacagcac cgaaccaccg ttcgcctgct caaagaaccc
     6241 tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt ctggtataga
     6301 cgcgcaattc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat ggcaggcgta
     6361 ggcaaaaaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg cgcttagcat
     6421 cgccagtttc tgcgcctgtt cgacaacgtg cttcattttc gggctgacgg cgacaatttg
     6481 gctgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca tacgaatcgt
     6541 tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat cctgaagata
     6601 aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct cgttatgcga
     6661 atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca attgtgcggc
     6721 ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc
     6781 catatccact ttgcttttca tatcgacaga gagcacaggt tcaggcaacg cctccagtaa
     6841 cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac gcacatcggt
     6901 aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa actccagttc
     6961 agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta aatcaatgcc
     7021 tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac
     7081 ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg atatgacact
     7141 atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga aaaacgggca
     7201 cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc tgggcgtttt
     7261 gcctttttgc agagtttctt tcacctgacc gataagctga cgacggaaat ccccaaggcg
     7321 aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt taatcccgag
     7381 tcgtaccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca aacgagcagc
     7441 gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg ctccggcaaa
     7501 agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc ccagttcaat
     7561 gccatacagc gtggcaatgc gattaattaa acgcagattg cgccaggcga taaacgccat
     7621 atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg ccgaatggct
     7681 gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat acaggctgac
     7741 cacttcacga tcgttctgcg tttcatggat tgaggcatac cagcgttgca gcgccggatg
     7801 cgactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcgac ctttgcccgt
     7861 gccgtggcta tgcaataaat cacgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg
     7921 ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa tcaatgcccc
     7981 agcggcacat ccacccagcg ctacccagtc ctgggtttgc caggcattca ttgtccactg
     8041 tataccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca ccattttgcg
     8101 ccacaggctg cgtttcggac gtaacgctgc gtccattacc gcttcgactt gcccctcttc
     8161 ttcctgcgct tcgtcgagcg tggccggggc aaaattttgc gcctgatttt cgtcaaaggt
     8221 ctgctgcgcc ctgaattttg gattctgttc gacctccagc ggaccgtcga aatcaatacg
     8281 tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag cgcggcatca
     8341 agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa cgcttcaaac
     8401 tggaaccctt gcttgtccca gaacgcctga ccgggcaaac gcgcaggaac ttcaccgggg
     8461 taaacggtga gaggcgcacc atcgctgagc cggttaccgc gcaatgccgg aattttctcg
     8521 ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc caggcaatcc
     8581 atactaatcc tttcaaacgc cgcattttgc caggcatcct gaatcagttg ttgcagtaat
     8641 gaaaccatat tggcgtgctg atcgaccgtt acatggtccg ctttcatcgc ggcaaacagc
     8701 agtttgtcga taaccggcga gaataagcgg cgaaacagcg tgcgctgccc gtagtggaaa
     8761 ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg cccactgttg
     8821 agaggttgca ggcaatccac cagcacaatc tggcggtcaa agcgcagaaa atgattctta
     8881 tagaacccct tcaccacttt ctcgcagtaa taattaaacc gctcgcgcag cattccggca
     8941 ttggtgtgct tatcggcctg cgccagtttg gactcgtccc aggcatcgac atccggccac
     9001 gggaagaatt gcagcgcggg cgcacctgcc atatctcccg gcaagacaaa gcgcccaggc
     9061 tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca cacggcggca
     9121 atgtctgcca gccggttttc gtcggcaggc gctagcgggt caagtccttc gcacatcatt
     9181 cgccatttcg ccgaccattc gccgcgctga ccattgagta agcccgtcat ctggcgcgac
     9241 cagcttaagt aatcctgcgc cagcatcggc aggtcgagca accattcgcc gggataatcc
     9301 acaatttcca gatacagcgt ggaggtatcc ttaaagtggc gcagcagcga atcgttcgat
     9361 ttaaaacgta acgccaggcg aatttcactg acgccgcgcg taggcgtcgg ccaggcggga
     9421 ggatcaccat ataactgcgc cagtccttca tcataggtga agcgcggaat gccaaagtca
     9481 cgctggggaa tgcgttttac gcccagcaag cgctcttcac gcaccgcgct taacagcggc
     9541 aaacgtgctc cggcatgaat attgagcaac tgattaacca tcgcggtgat aaacgctgtt
     9601 ttgccgctgc ggctaagtcc ggttacagcg aggcgcagat gtctgtcgac accccgattc
     9661 accagcgcat tgagttcatt tttaagtcgc ttcattgctg tcctgtgctg ctctgaagac
     9721 cagaaaaaaa atcattcgta aggatatgaa tgtgggggcc aggccattga ttatcaactg
     9781 cccgtgttgt catcgacggg cgaactccat aagatgtgca gcattcacgt tcggacggag
     9841 ttcctgacct atgtccccta ctatttatga tattgccagg gttgcaggcg tatcaaaatc
     9901 caccgtatca cgcgtgctga ataagcaaac taatatctcc ccggaagcgc gcgaaaaagt
     9961 gttacgggcc attgaagaat tacagtatca gccaaacaag ctggcccgcg cgctgacctc
    10021 ttcgggtttt gatgccatta tggtgatttc tacccgttcg accaaaacta cggcgggtaa
    10081 tccgtttttc tctgaagttt tgcatgccat caccgccaaa gcggaagaag aaggtttcga
    10141 cgtgatatta caaacgtcgc acaatccggc agaagatctg caaaaatgcg aaagcaaaat
    10201 taaacagaaa atgattaaag gcattattat gctcagttcg ccagcggata agtcattttt
    10261 tgcccaactc gataaatacg atattccggt ggtggtaatt ggcaaagttg aaggtcaata
    10321 tgcccatgtt tattctgtcg ataccgataa ttttggtgac agcattgcgt tgaccgatgc
    10381 attaattgaa agtgggcatc aaaatattgc ctgcctgcat gctccgcttg atgtccatgt
    10441 ttcggttgat cgcgttaatg gctataagca aagtctgtcc gcgcataata ttgcagtgcg
    10501 tgataaatgg attgttgacg gcggttatac ccatgaaaca gcactgcaag cagcacggca
    10561 attattaagt cagtcaccgt tgcctgaggc agtgtttgcc actgacagcc tgaaattaat
    10621 gagcatttat cgtgcggcgg cagagaaaaa tattgctatt ccgcagcagt tagcggtggt
    10681 gggttatagt aatgaaacgc tgtcgtttat tttaacgcct gcaccaggcg gcatcgatgt
    10741 tccgacgcag gagttaggtc aacaaagctg cgagttatta ttccgcttaa tttcgggaaa
    10801 accgtcacca cagaatatta ccgttgccac gcatatgacg ctgaaataaa taccagctac
    10861 cttgcctgaa tgccggatgc gatgctggcg catcttatcc ggcctacatt tatagatcgg
    10921 ataaagtact cgcgccgcat ccggcaattt agcccattat cagaacgagt aatttacgcc
    10981 gacacctgca taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc
    11041 gtattccagc gaaacggaaa gtccgttctg gaaatcataa ccgtaaaata aacccacacg
    11101 gttaaaatca tggccttcac gttcaatatc atcctgccag tcccagttac tctagcgatc
    11161 cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc
    11221 gcgaatttct tgtgtggaaa actcaccgtt attgcggctg tcgtccatat tgaagccgcg
    11281 ctcgagataa tagttcactc gcaaagcaac cgtttcgttg aaggtatatt gcagacctgt
    11341 ttccgtttcg acacgggtat cagcgtaacc ggtcgtgttc aaatcgttgg caaatttata
    11401 catcgacaac caaccgttga aacgtaaatc atcagtcagt ttcacatccc agtctggcgc
    11461 gattttccag cgctgcatat tcgccgtgtc tttgcccggt tcatcaacgt agtgataacc
    11521 ataattacgg aaaccgccgg tcaggccgaa actgaaatca tcgctttcga ggaactgata
    11581 atgcacctcc agctccgggc gatcaaacct cgttccacgt ttacccgcgc tataatctac
    11641 cggcccttcc tgataatagg ccagagcaat tctccacggc ccgttggcgg cattaaaata
    11701 gactgaaggc tccgccagcc catccatatc ttcgccataa ccctcgacgt tttctatttc
    11761 gtacatcgca ccgatattaa agtgccagtc gctcttttcc tcggcctgtg cgcaggccat
    11821 tcccgcacac atcaccagtg cggtacaggg taataacttt ttttgttcct tgtattatcc
    11881 tgggggaatg ccttccccct gtatttagcg aattgctatt tccgtttctg catcaaagaa
    11941 atgacatttc gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa
    12001 cgcaccagca cggaccacta actcgtgccc ccaaccgtgg tgtagagcat aaattccgca
    12061 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
    12121 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
    12181 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
    12241 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
    12301 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
    12361 ttcataatca caatccgcgc cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
    12421 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
    12481 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
    12541 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
    12601 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
    12661 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
    12721 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
    12781 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
    12841 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
    12901 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
    12961 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
    13021 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
    13081 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
    13141 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
    13201 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
    13261 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
    13321 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
    13381 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
    13441 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
    13501 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
    13561 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
    13621 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
    13681 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
    13741 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
    13801 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
    13861 tgccactgct caagtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
    13921 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
    13981 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
    14041 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
    14101 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
    14161 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
    14221 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
    14281 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
    14341 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
    14401 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
    14461 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
    14521 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
    14581 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
    14641 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
    14701 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
    14761 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
    14821 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
    14881 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
    14941 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
    15001 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
    15061 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
    15121 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
    15181 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
    15241 gtcactgtct cgcctgcatg aagctgcgta ctggtatgtt gcagtaaacg gcgctctttg
    15301 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
    15361 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
    15421 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
    15481 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
    15541 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
    15601 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
    15661 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
    15721 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
    15781 atccctcgcg tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
    15841 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
    15901 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
    15961 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
    16021 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
    16081 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
    16141 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
    16201 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
    16261 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
    16321 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
    16381 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatcagc aggccaccac
    16441 cctgcagttc tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc
    16501 gggcggtggt aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt
    16561 gcgtatcgag cgcaaacgat ggtgaaagtc gtaggccagc accttgccca gtttgcgcgc
    16621 ggtatcgcac atttcccacg cctgttctgt cgtcatggcg ggcggttttt cgcacatcac
    16681 atggcagcct gcttccagtg ccatcagggt atgttcgtaa tgaaaacggt taggtgagcc
    16741 gacgctaacc acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacacgga
    16801 tgcattcccg tatttttctg ccagcgcctg cgcctgggaa aggcggctgt cacagacagc
    16861 caccagttcc agatcgttgc gggtgcagta gtacgaagca tgaaccttat ccgccacctg
    16921 gcccgcgcct attatcgcga cccgcagcgg agagcttgtc attgcacttt tcacaaagac
    16981 ctcttagcag gtacgcaacc aggccaacga atcacggtac gcctgggcag gatcttctgc
    17041 ccggatacgc ccttcataca ccacataacc ctgatagtta tcggcgcgca gctgttcaaa
    17101 cagcgcgtgg aaatcgaggg tgccactgcc cggctggtaa cgatggttat cggcaatatg
    17161 cacatgaccg agcaagtcac ggttgtcgtg cagcgcctgc gccaggttat cttcttcgat
    17221 gttcatgtga tagaaatcgc cgataatctg cacatgttta agattgtttt cgacgatgta
    17281 acgacgagca tcggcgaggg tgttgatcat atgatcctga tagcggttta acggttcgag
    17341 atacaccacg gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac
    17401 cagtttgcgg tcgccgtcca ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa
    17461 catgccccac gctgccggaa accccccact tctgccagcg cctcgagaat gcgttcgatc
    17521 tgctttaagc cattaagacg acgctcttcg ataaagtcac caatccagcc gtcatagccg
    17581 ccgcaggcgg tggtcactgg taaaccggtt tctttgatcg ccgctttgac ttcttcgatg
    17641 ttgttgacca gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa
    17701 cgaaatctct cgcggatatt ttccggaaag aatgcctgat tctgtgtgcc gattttcatt
    17761 ttttcattcc tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat
    17821 cgacatactg catgtagctt tccgggctgt tggcaaaggt cactaccggg tcgattaaat
    17881 cttcgcaatt gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac
    17941 ggctccagcg cggataatcc gggttcggtt cgctgcacgc gcgggagaaa acaattttgg
    18001 cgttattgaa atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg
    18061 cgacatagga gatggtgccg ccgtaggcca ggccgcgcag cgccgattgc agcgcgtcgg
    18121 cgtaaccgct ggtttcgata atcacatcag ccccctgctt gccggtcagc gttttgatct
    18181 ctttgcctac gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa
    18241 tatcacagcg atgggcgata ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg
    18301 ccagttggat ggcaatttga ccgatcgcgc caagccctac caccacgaca aaatccccta
    18361 cgcgtacgtt ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacagacgg
    18421 cgtttttcca ggagctgcct tgcggcattt tgcgcagctt gtagttattc actgcgttaa
    18481 tgatgaccgt ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtctg
    18541 taacgtcgct gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc
    18601 caaactcaat gccgcgcggc gcatctgccg gacgcggggt gaacatctgc cactcgccgt
    18661 taaagtcttc atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt
    18721 tcggtgcgcc gaaacgcacg cggatcttca cttcattggc taaaatcgcc cgatcttcat
    18781 actcaaccag cgcagcaaca cgcggtgctg tggctactaa ctttttcatg taaaactcct
    18841 tgtaaagaaa ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa
    18901 aacgttccga aagcgcatac ataatcacca ccggcaatgc ggtcaccagc gaggccgcca
    18961 tcatccgtcc ccagatgtaa tcaggcgtac tgaacagcgc gttcaggccc actggtaagg
    19021 tgaaattgct ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca
    19081 taaagcagta gacgaacacc gatatcagcc cggacatcgc caggggcacg gtgatacgga
    19141 agatgatttg cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcaggga
    19201 tggtgtcgaa gtaacttttc agcataaaca cggcggtggg cagggtttgc gtcaccatgg
    19261 taatgatcag cgccatctcg gtgtcgtaaa taccaagcgc ggtgatgatt ttgaacagag
    19321 gcaccaccag caaaatgcca gagaacatat acaccgtgta aaagctggcg ttgatggtca
    19381 tccggccttt aaagcgcagg cgggaaagcg cgtaagctcc gagaatgccg agaaataccg
    19441 ccaccacgga ggaaaccacc gacaccacca tgctgttacg gaagtagtcg acaaacggga
    19501 aaatcatcgg gttaaaaatg tcgacgtaat gctccagcgt ccactgttgc ggcagcagcg
    19561 taggatgcag cgatatcgcc tctttcgcgc ttttgaacga ggtcatcagc atcacaaaaa
    19621 acggaaacag cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atacgactga
    19681 gcgtgcgttt atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg
    19741 aaaataatga tgaagagcac caccgagatc gccgccgctt tccctaagtc attaaaggca
    19801 aaggcggttt tgtagagata gacaccaaga atatcgacct tggttgtcag cagataaaca
    19861 tcagcgaaca tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc
    19921 ataattgcgg gcagcgtgac gatgcgaaac cgctgccagg cattagcgcc atccatctct
    19981 gccgcttcgt acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca
    20041 tacgggaagt agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta
    20101 tcaaaccaca gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca
    20161 atgccgtagc cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt
    20221 acgtaggaga ggatcaccag cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa
    20281 aacatcgcca cggccagccc gagcacggtg ctgcccgcca ccaccagcgc cgtataccag
    20341 accgtcatcc acagcgagtg ccagaagccg ggatcggaga gaatgcgcac atagttactc
    20401 accccaacaa acgttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg
    20461 atattcgaca ccatcggcca cgccaccaga ccgcccagca gtaataagct gggcgcgaga
    20521 agcagcagcg caaacggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc
    20581 tgtcctgggt ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg
    20641 tgccagggag atcggcttta cccacggtga cgttatgcac catggagctc accacaccag
    20701 aacccgtcac atcgcccatg cgggtaaagt ttttatcccc tactgcgcca aagacctgaa
    20761 tatttggcag ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt
    20821 tgtctttcca ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg
    20881 acatcatcac ccagtcggca atgttgtccg cctgctccat aaaggtgaca aatttttctg
    20941 ctgcttcggt cttttgtccg gcggtaatgg tcagcgaggt caacatgccg tagaccgcag
    21001 agtttttctc ggttggcacc acgaaaccga cgtttttcgg gtcgccttct ttaatcacag
    21061 ccggaaggat ataggtggag taaatcgcca tcggcgcggt gccgttcata aaggcgtctt
    21121 tcacttccat gatgtcgtta gaacccggca tggtgttggc agcaaggtcg cggtaatagg
    21181 tcagcgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg ttaaagacgt
    21241 tggcctagtt ggataacgca aactgggaga aggattgttc cgtcagcacg ctttctgccg
    21301 taggcagcgc aataccgtat tttttattcg ccgggtcatt cagtttctgt gcaacgtcca
    21361 gcagctgttg ccagtttttc ggctcctcca gccccgcttt tgccagcaca tctttgcgat
    21421 accagatgtc gccaatccag gcgctgacag gaacaccggt ccatgcgcta ccatcttcgg
    21481 tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg ctgatgaccg
    21541 tggcaaccgc tttgcgatcg ataagctgct ctttatccat aactttggcg tagtcatggc
    21601 tggtttcgat cacttccggc agcgagccgc tacgtgaaag ggtgatgact ttggtgttat
    21661 gagcgtcttc ttccacgggc acttgtttaa cggtgatacc tgggttttct ttttcaaaac
    21721 gggcaatcaa tttgctgata actgcctgac gctcctgctc caccgaagaa tgcataaatt
    21781 cgatggatac atgggtttta ttttcttctt tacagcctga aatcagggcg caggaaacca
    21841 gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta tttaatccac
    21901 ataacctgcc aggggcgcag cgttatttca ttacccaata tatcaacttc actaatcaga
    21961 tcccgaccat gcagatcagt aatatttaca tgctgaatat ttttactgac attaaacaat
    22021 ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga tgaattaacc
    22081 gtatcgatgg taaaattatt atccggatga aactcgttat ggctgcgacg aagtgtaatt
    22141 aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt cagttctcgt
    22201 gttatctctt cactgtgata ttttttacgg ttaatcgcac ggttatatcc gagtttttcg
    22261 acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc gggtacaccg
    22321 gggaaactta acaaaatcgc atgggcaagg ataaacctgg tgcaacgttc ttcatcgcta
    22381 ctctcgcggc ggcttaacgc attcatatag gtgacattca tctcataggg actgcgcgtg
    22441 ccgtcgggat tattttt
//
LOCUS       DAJTNW010000066        22249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNW010000066 DAJTNW010000000
VERSION     DAJTNW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..239
                     /locus_tag="OO179_002675"
     CDS             <1..239
                     /locus_tag="OO179_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5532677.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="OO179_002676"
     CDS             249..887
                     /locus_tag="OO179_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5532678.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="OO179_002677"
     CDS             890..2053
                     /locus_tag="OO179_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5532679.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..3762
                     /locus_tag="OO179_002678"
     CDS             2137..3762
                     /locus_tag="OO179_002678"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5532680.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="OO179_002679"
     CDS             complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="OO179_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5532681.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="OO179_002680"
     CDS             complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="OO179_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5532682.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4814..5563
                     /gene="yjfP"
                     /locus_tag="OO179_002681"
     CDS             4814..5563
                     /gene="yjfP"
                     /locus_tag="OO179_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5532683.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="OO179_002682"
     CDS             complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="OO179_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5532684.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="OO179_002683"
     CDS             complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="OO179_002683"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5532685.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7842..9239
                     /gene="ulaA"
                     /locus_tag="OO179_002684"
     CDS             7842..9239
                     /gene="ulaA"
                     /locus_tag="OO179_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5532686.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9255..9560
                     /gene="ulaB"
                     /locus_tag="OO179_002685"
     CDS             9255..9560
                     /gene="ulaB"
                     /locus_tag="OO179_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5532687.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9570..10034
                     /gene="ulaC"
                     /locus_tag="OO179_002686"
     CDS             9570..10034
                     /gene="ulaC"
                     /locus_tag="OO179_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5532688.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10048..10698
                     /gene="ulaD"
                     /locus_tag="OO179_002687"
     CDS             10048..10698
                     /gene="ulaD"
                     /locus_tag="OO179_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5532689.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10708..11544
                     /gene="ulaE"
                     /locus_tag="OO179_002688"
     CDS             10708..11544
                     /gene="ulaE"
                     /locus_tag="OO179_002688"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5532690.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11544..12230
                     /gene="ulaF"
                     /locus_tag="OO179_002689"
     CDS             11544..12230
                     /gene="ulaF"
                     /locus_tag="OO179_002689"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5532691.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12359..12634)
                     /locus_tag="OO179_002690"
     CDS             complement(12359..12634)
                     /locus_tag="OO179_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5532692.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12961..13356
                     /gene="rpsF"
                     /locus_tag="OO179_002691"
     CDS             12961..13356
                     /gene="rpsF"
                     /locus_tag="OO179_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5532693.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13363..13677
                     /gene="priB"
                     /locus_tag="OO179_002692"
     CDS             13363..13677
                     /gene="priB"
                     /locus_tag="OO179_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5532694.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13682..13909
                     /gene="rpsR"
                     /locus_tag="OO179_002693"
     CDS             13682..13909
                     /gene="rpsR"
                     /locus_tag="OO179_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5532695.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13951..14400
                     /gene="rplI"
                     /locus_tag="OO179_002694"
     CDS             13951..14400
                     /gene="rplI"
                     /locus_tag="OO179_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5532696.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14471..15034)
                     /locus_tag="OO179_002695"
                     /pseudo
     CDS             complement(14471..15034)
                     /locus_tag="OO179_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15098..16245
                     /locus_tag="OO179_002696"
     CDS             join(15098..15335,15335..16245)
                     /locus_tag="OO179_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5532697.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            complement(16264..16467)
                     /locus_tag="OO179_002697"
                     /pseudo
     CDS             complement(16264..16467)
                     /locus_tag="OO179_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(16614..17516)
                     /locus_tag="OO179_002698"
     CDS             complement(16614..17516)
                     /locus_tag="OO179_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5532698.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            complement(17726..18472)
                     /gene="dsbG"
                     /locus_tag="OO179_002699"
     CDS             complement(17726..18472)
                     /gene="dsbG"
                     /locus_tag="OO179_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5532699.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18844..19407
                     /gene="ahpC"
                     /locus_tag="OO179_002700"
     CDS             18844..19407
                     /gene="ahpC"
                     /locus_tag="OO179_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5532700.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            19652..21217
                     /gene="ahpF"
                     /locus_tag="OO179_002701"
     CDS             19652..21217
                     /gene="ahpF"
                     /locus_tag="OO179_002701"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5532701.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(21338..21766)
                     /gene="uspG"
                     /locus_tag="OO179_002702"
     CDS             complement(21338..21766)
                     /gene="uspG"
                     /locus_tag="OO179_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5532702.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            21987..>22249
                     /locus_tag="OO179_002703"
     CDS             21987..>22249
                     /locus_tag="OO179_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5532703.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
BASE COUNT         5429 a         5306 c         5821 g         5693 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac cgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc
     3421 aatattatgt gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta
     3481 ttgtcggaag catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt
     3541 cgtttacagc agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag
     3601 ctattcctgc tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag
     3661 gcgtggtgtc aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag
     3721 aatgatttat tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg
     3781 acgctggttt cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg
     3841 cgccgcatcc ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt
     3901 acgcgccagt tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca
     3961 ccttgttcat cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata
     4021 ttgctaactg agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat
     4081 tctgcagact gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca
     4141 agtaattgtt tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat
     4201 cgcggtaata aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag
     4261 ggacgcaaag caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat
     4321 ggcttgtgaa taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata
     4381 atcagctttt gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc
     4441 ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat
     4501 ctcttgcgga tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca
     4561 ggcacttaac atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt
     4621 cctgctaacc atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt
     4681 tacctggtac gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt
     4741 atgaactaat accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa
     4801 aaaggagaga cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca
     4861 tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt
     4921 tacttcatcc agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg
     4981 ggtgatcatg ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg
     5041 gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc
     5101 ggcaatagcc gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat
     5161 gggcgcgatg acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag
     5221 catgatggga tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc
     5281 tgaaacggca gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga
     5341 agcgacaaac cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga
     5401 cgatgacgtt gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg
     5461 acgggataaa ctgctaacct gttcatggca gccaggcgtg cgtcaccgta ttacgcctga
     5521 ggcgttagat gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac
     5581 cccttgcgct tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat
     5641 atcgatttga tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc
     5701 aaccagtacc accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag
     5761 catatcggtt ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg
     5821 cccggcatag agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt
     5881 gtactgtcca cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag
     5941 cggtagataa ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa
     6001 cgcggtggag ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc
     6061 tttagcaata cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca
     6121 gcgcgggcgc tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct
     6181 ttcgccaagt ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac
     6241 gactttctca acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct
     6301 ttgtgcttca gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat
     6361 agccaggaaa tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca
     6421 gattacagga atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg
     6481 cgcggatagt ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc
     6541 tgccagataa acggcttgaa gccatacttc agacgatctt ttttgatctc ccacagcacg
     6601 cggatctctt gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc
     6661 actttcgcat tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta
     6721 ataccgcgcg ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg
     6781 tgcttcgcat aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg
     6841 gttttgaaca ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct
     6901 ttttgatcgg caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt
     6961 tcaatgtctt tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt
     7021 acgccccagc caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca
     7081 gcacagttct gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga
     7141 gtcgccagta ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg
     7201 cgtaggtttg gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc
     7261 attaacgggt taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg
     7321 gtgccacctt ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg
     7381 ccaggggcga cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa
     7441 gtgctcagga tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt
     7501 catgatagtt caattcgaat caacatgtga ttggttttga ttaatcctga cactattcct
     7561 tcaggaaggc aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt
     7621 gtggaaagat ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg
     7681 tttcgttgtg tgaatcacgc tctatccgtg tggaattatt tgcgggtcgc gccacattta
     7741 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtacac aacattccgg
     7801 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
     7861 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
     7921 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
     7981 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
     8041 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt
     8101 acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg
     8161 cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta
     8221 ttcgtaccat catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta
     8281 cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt
     8341 cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg
     8401 gctgcggttt ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg
     8461 cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga
     8521 atattttcca cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca
     8581 ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg
     8641 tctatatcct gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg
     8701 tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc
     8761 ctggtgcggt tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg
     8821 tctggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct
     8881 gcggttcctc gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca
     8941 tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga
     9001 tggggatgat cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct
     9061 ggatggggat ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca
     9121 tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg
     9181 gccgcgcact gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat
     9241 aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt
     9301 ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg
     9361 taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg
     9421 cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc
     9481 gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt
     9541 tcccgcagga tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata
     9601 aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggcgttg
     9661 acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg
     9721 ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc
     9781 cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt
     9841 tcaaccacga agataacgac ccggtggata tcctcatcac catggcggcg gtcgatgcca
     9901 acacgcacca ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt
     9961 tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca
    10021 acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac
    10081 aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt
    10141 atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa
    10201 gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt
    10261 ctttctcgta tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat
    10321 atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc
    10381 gaactgaccg gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag
    10441 caggtggttt atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc
    10501 gatatcactg cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc
    10561 ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt
    10621 cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc
    10681 gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat
    10741 gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg
    10801 ggcttcgatt ttgtcgagat gtcggtagat gaaactaacg agcgcctgtc gcgcctcgac
    10861 tggagccgcg aacagcgtct ggcgctggtg aatgctattg ttgaaaccgg cgtgcgtgtg
    10921 ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg
    10981 cgggcgcagg ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt
    11041 cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt
    11101 cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc gcaggtgacg
    11161 ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac
    11221 gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg
    11281 tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat
    11341 gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat
    11401 ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag
    11461 atgtggagcg aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa
    11521 gcaggcatgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca
    11581 tggatctgcc gcgctatgtg ctggtgacct ttacttgggg caacgtcagc gctatcgacc
    11641 gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag
    11701 acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt
    11761 ccgacactgc gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc
    11821 atacccactc cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag
    11881 gcaccacgca cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag
    11941 aagaggtgca gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca
    12001 acgccgagcc gctgcatacg ccggaaattg tggtgtatca gcacggaccg ttcgcctggg
    12061 ggaaagatgc ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg
    12121 cgtggattgc gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata
    12181 aacacttcat gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct
    12241 gcggaaattt ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa
    12301 gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct
    12361 agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa
    12421 ttacgtggta gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt
    12481 cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca
    12541 tatttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa
    12601 gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg
    12661 tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg
    12721 gtgaccattt ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa
    12781 atcagcggtt tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt
    12841 tagcggggta tgccagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    12901 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    12961 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    13021 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    13081 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    13141 ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    13201 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    13261 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    13321 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    13381 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    13441 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca
    13501 tggtgtcaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    13561 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga
    13621 ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg agactagcca
    13681 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    13741 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    13801 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    13861 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    13921 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    13981 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    14041 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    14101 cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag
    14161 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    14221 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    14281 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg
    14341 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    14401 ttcgttattc aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt
    14461 tcgtcaggca ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag
    14521 gatggtttgc tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc
    14581 gctcgaaacc taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt
    14641 tcaaatcatg gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg
    14701 cattagaaac gcctgtaaca gtgtgaagtt ggtcgatata aagatcgatt gttggtagtt
    14761 cagatgacca gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc
    14821 cggtatagtc tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac
    14881 tgttgggttc gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg
    14941 cacccgcgta agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt
    15001 tccatccaaa agaattatca ccattcattg ttactgaatc gccacgggtt taacagacac
    15061 ctcagagtca tttaagatgg cttaaagaga ggtgcccatg agcggtaagc gttatcccga
    15121 agagtttaaa actgaagcag tcaaacaggt tgttgatcgc ggttattctg ttgccagcgt
    15181 tgcaacacgt ctcgatatca ccacccacag cctttatgcc tggataaaga agtacggtcc
    15241 ggattcttcc actaataaag aacagtcaga tgctcaggcc gagatccgcc gtctccagaa
    15301 agagctgaaa cgggttaccg acgaacggga catattaaaa aaagccgcgg cgtacttcgc
    15361 aaagctgtcc gactgaggta cgcctttatc cgtgacaact cctgttgctg gcctgttcgc
    15421 ctgctctgtc gggtgctgga tgttcatccc agtggttttt acgcctggct tcagcagccg
    15481 cattcacaac gccatcaggc agacctgaga ctgacaggac agattaaaca gttctggctg
    15541 gaatcgggat gcgtctatgg ttatcgcaaa atccatctgg atctgcgtga cagcgggcaa
    15601 cagtgcggag taaacagagt ctggagactg atgaaacgtg tcggaataaa ggctcaggtc
    15661 ggataccgaa gcccgcgggc acgtaaaggc gaggccagta tcgtgtcacc caacaggctc
    15721 cagcgacagt tcaatccgga tgctcctgat gagcgttggg taacggacat aacctacatc
    15781 aggacccacg aaggctggct gtatcttgcc gttgttgttg atctgttctc acgcaaaatt
    15841 atcggctggt ccatgcaatc ccggatgaca aaggacattg tcctgaacgc actgctgatg
    15901 gctgtatggc ggcgtaatcc cgaaaaacag gtgctggttc attcggatca gggcagtcag
    15961 tacacaagcc atgagtggca gtcgttcctg aaatcacacg gcctggaggg tagcatgagc
    16021 cgtcgcggta actgccatga taatgcggtt gcagaaagtt ttttccagtt gttgaaacgt
    16081 gaacggataa agaaaaagat ctacggaacg cgggaagaag cccgcagtga tatttttgat
    16141 tacatcgaaa tgttttataa cagtaagcgt cggcatggtt ctagcgaaca gatgtcaccg
    16201 acagaatatg aaaaccagta ttatcaacgg ctcggaagtg tctagattat ccgtggcgat
    16261 tcattgcgcc tcccagttga taaacaaaac gcagattttt ttgcccattt gtcgggcaag
    16321 ttcggctgtc agatgcagca ttaaaccaga gtctttgccg ccagaaaatg agacacaaac
    16381 gcggggtagg gtgttgagtg tctaggtaat acgctctcgt gcagcttcca gaatattgag
    16441 cggaagagga attttataaa tagacatctg aagacgctct ctgaacgtta cagggtgaac
    16501 agtgagataa gactgttaat tataaagtaa acactgtaaa gcagagtgtt ttatttttca
    16561 ttcgcgctgt gaataaatag atgaaagtta tagtagctgt ctatagttat atatcaaaaa
    16621 ctctcattta gacggtcaat aaatcgggag aacaccaaat tatttaaaga agatttatta
    16681 taggaaatgt aaagctttat tgaaggtaac ggatgttcta gttttatctc ttttaaatta
    16741 agaaagtcac ggtggtagtt ataaagttca taggggacta ttcccagtaa atctgtaact
    16801 gcaatgctat taattattgt cagcaaagag gaaccacgaa atgaaatttt gcgattcata
    16861 aacttctcgt ctatttccat ttgaatatca tcaactcctg ctgatttggc tattaattgg
    16921 gtgaactctt ctgccataat ttgttcataa gtgctattgt cagtaattcg cggatgctta
    16981 ttactacaaa ttagagtatt gcgaattgta tgcaatggca tgcaaatgac ggaacggctg
    17041 atcactggca tttgggtgat caccagatcg gctttgcggt gaaccaacag ttcttcagca
    17101 ttctctgctg acatgaggat atcatggcac tcaatctcga cagaggcatc ttgccgtagg
    17161 cagcggatca gcatactatt attagagcaa gaaatgagct gagggccgta gataataaag
    17221 tttttcttta gttcggactt attgactata ttaattgttt gttcaaggcc tttgaggttt
    17281 ttctcaagat ggtgatgcag attcaaccct gttgtcgtcg gcgcgatacc ttttcctgag
    17341 cgaatgaata aagggtcatt aaattgcgca cgtaagcgtt gtaacgactg gctcacggct
    17401 gatggggtga tatatagtga ttcagcagct ttgctaatgc ttaaatgctg gtaaatacac
    17461 tcaaatatga caagaagatt taagtcgaac tttttcaagt cgtagagatt ggccatacac
    17521 tactccggtt tatatttttc ttccatggta tattcactta gtcaatgttt tttgcgtatg
    17581 tatttttatt gattatgttt ttttgcttaa ctcaggatta agtttttcta aaatagcaca
    17641 gccacttagg gtagagcgag aaattcatgc ttttttagga tggttgaaaa gcagggatat
    17701 agttcaacca tccatgaatt accttttatt tatttcccat aatgatatta agcgttttct
    17761 gatcgggcaa ccccacggcc tgttgcagcg tattttcctt actcatgtaa tagatagccg
    17821 gcgtgacatt tgcccccaga tcgtccatca gtttctcatt gtcacttaac actttcattt
    17881 gctctgtact tacgtttgca ggcacgttta gcttaagctt gcaaccagag gcttcatatt
    17941 gttgccaggt ttttgcggga tctttggagg caagaattgc cgctgctgtc gccgggcttt
    18001 ctggcttgat aaccccaacc aacaatgttc ttaattgcac tttgccagaa tctacccacg
    18061 ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa cggatcggag aagacgtaga
    18121 caatgaccgg cgcatctttt ttaccgtcga ggagccagtg ggattgttcc atccgttgcc
    18181 acatttcgcg tccggctggt gcgtaaattt ctttttcgat aagtgtgtta ctcaggtttt
    18241 cacctttctc gttgtacatg taaccagaga tagcgtgctt accatcggga gtcaggtaga
    18301 tggtgacgcc catatcctga tactttccga gataaccttt cattcctccg ggggcatcga
    18361 acgttttgat gattgtaatg ccctgttttt caatcgcttt tactggggaa ggaagttcct
    18421 ctgcgaaggc gattgcagga agcagagcca gtaaaagtat cttttttaac attaatttgt
    18481 tcttttcagt cagtgcaaaa gtcgagtaaa aggcataacc tatcactgtc ataggtaaga
    18541 gcttagatca ggtgattgcc ctttgtttat gagggtgttg taatccatgt cgttgttgca
    18601 tttgtaaggg caacacctca gcctgcaggc aggcgctgaa gataccaaag ggtagttcag
    18661 attacacggt cacctggaaa gggggccatt ttacttttta tcgccgctgg cggtgcaaag
    18721 ttcacaaagt tgtcttacga aggttgtaag gtaaaactta tcgatttgat aatggaaacg
    18781 cattagccga atcggcaaaa attggttacc ttacatctca tcgaaaacac ggaggaagta
    18841 tagatgtcct tgattaacac caaaattaaa ccttttaaaa accaggcatt caaaaacggc
    18901 gaattcatcg aaatcaccga aaaagatacc gaaggccgct ggagcgtctt cttcttctac
    18961 ccggctgact ttactttcgt atgcccgacc gaactgggtg acgttgctga ccactacgaa
    19021 gaactgcaga aactgggcgt agacgtatac gcagtatcta ccgatactca cttcacccac
    19081 aaagcatggc acagcagctc tgaaaccatc gctaaaatca aatatgcgat gatcggcgac
    19141 ccgactggcg ccctgacccg taacttcgac aacatgcgtg aagatgaagg tctggctgac
    19201 cgtgcgacct tcgttgttga cccgcagggt atcatccagg caatcgaagt taccgctgaa
    19261 ggcattggcc gtgacgcgtc tgatctgctg cgtaaaatca aagcagctca gtacgtagct
    19321 tctcacccag gtgaagtttg cccggctaaa tggaaagaag gtgaagcaac tctggctccg
    19381 tctctggacc tggttggtaa aatctaaatt tccttcgtct ttcacgccat agcggcgttg
    19441 gcgtcgcccg ctcaccccgg tcacttactt gtgtaagctc ccggggattc acaggctagt
    19501 cgccttgctc tgacgcgaaa tacttcggaa attcacctaa ttcttcgggt gctgcggcac
    19561 ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgcag aggccgcttg
    19621 catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga aaactcaact
    19681 caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc tggatgacag
    19741 cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt cagacaaagt
    19801 cacctttaaa gaagataaca gcttgccagt gcgtaagccg tctttcctga tcaccaaccc
    19861 aggttccaac cagggaccac gttttgcagg ctccccgctg ggccacgagt tcacctcgct
    19921 ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt ctctgctgga
    19981 gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc tctcttgcca
    20041 taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtattgaacc cgcgcatcaa
    20101 gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca acgtgatggg
    20161 cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga cgttgactga
    20221 aatcgttgcc aaaattgata ctggcgcgga aaaacgtgcg gcagaagagc tgaacaagcg
    20281 tgatgcttat gacgtattaa tcgttggttc cggcccggcg ggtgcagcgg cagcaattta
    20341 ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg gtcagatcct
    20401 cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc agaagttggc
    20461 aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca gccagagcgc
    20521 cagcaaactg atcccggcag cggttgaagg tggtctacac cagattgaaa cagcttctgg
    20581 cgcggtactg aaagcacgca gcattatcgt ggcgacgggt gcaaaatggc gcaacatgaa
    20641 cgttccgggc gaagatcagt atcgcactaa aggcgtgacc tactgcccgc actgcgacgg
    20701 accgctgttt aaaggcaaac gcgtagcggt tatcggcggc ggtaactccg gcgtggaagc
    20761 ggcaatcgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg cgccagaaat
    20821 gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg acattattct
    20881 gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc tggaatatcg
    20941 agatcgtgtc agcggcaata ttcacaacat cgaactggcc ggtatttttg tccagattgg
    21001 tctgctgccg aacaccaact ggctggaagg cgcagtcgaa cgtaaccgca tgggcgagat
    21061 tatcattgat gcgaaatgtg aaaccaacgt caaaggcgtg ttcgcagcgg gtgactgtac
    21121 gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag cctctctgag
    21181 tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat tcacctgcaa
    21241 ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa gccctgccgg
    21301 gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaacgacc agtacaggca
    21361 gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc gaaatcgatg
    21421 ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac tcattgactt
    21481 catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg atggtgaagt
    21541 ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct tcaaaacgac
    21601 gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt acgtgaagta
    21661 gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct ttgtcgctca
    21721 attccatttc aaatacatca actggcataa tgattgcctt atacataacc ctttctccct
    21781 gttaatcatg aacaaatcat tctccatgat tataatattt atccctgata tttgtctggt
    21841 tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac taggaattct
    21901 cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa agactgactc
    21961 ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc ccacatcacg
    22021 ttcaggtaga aaatgttccc gatccgggca ttgaacaggc agatgatatt attctgcgta
    22081 ttacggtaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa ataccccagg
    22141 ttaaacatgg cgatattttt ggtcatgaat ttatggggga agtcgttgaa accgggaagg
    22201 acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gttcgtcat
//
LOCUS       DAJTNW010000067        22103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNW010000067 DAJTNW010000000
VERSION     DAJTNW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO179_002704"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO179_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5532704.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="OO179_002705"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="OO179_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5532705.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="OO179_002706"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="OO179_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5532706.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO179_002707"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO179_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5532707.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="OO179_002708"
     CDS             3251..5011
                     /locus_tag="OO179_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5532708.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="OO179_002709"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="OO179_002709"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5532709.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO179_002710"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO179_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5532710.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO179_002711"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO179_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5532711.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO179_002712"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO179_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5532712.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO179_002713"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO179_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5532713.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="OO179_002714"
     CDS             complement(9679..11586)
                     /locus_tag="OO179_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5532714.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO179_002715"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO179_002715"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5532715.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="OO179_002716"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="OO179_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5532716.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO179_002717"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO179_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5532717.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO179_002718"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO179_002718"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5532718.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="OO179_002719"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="OO179_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="OO179_002720"
     CDS             complement(17598..18113)
                     /locus_tag="OO179_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5532719.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="OO179_002721"
     CDS             complement(18121..18663)
                     /locus_tag="OO179_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5532720.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="OO179_002722"
     CDS             complement(18761..19744)
                     /locus_tag="OO179_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5532721.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..>22103)
                     /locus_tag="OO179_002723"
     CDS             complement(19735..>22103)
                     /locus_tag="OO179_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5532722.1"
                     /translation="LNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPAF
                     KQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINAL
                     FSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYLQ
                     RAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSNA
                     QITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVPL
                     LQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAMF
                     GLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYST
                     SGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGLF
                     YLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSLP
                     LNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGYN
                     SEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSLG
                     ETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLEN
                     AVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDG
                     SLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5477 a         5793 c         5194 g         5639 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaa
//
LOCUS       DAJTNW010000068        21504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNW010000068 DAJTNW010000000
VERSION     DAJTNW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(38..2107)
                     /gene="glyS"
                     /locus_tag="OO179_002724"
     CDS             complement(38..2107)
                     /gene="glyS"
                     /locus_tag="OO179_002724"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5532723.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="OO179_002725"
     CDS             complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="OO179_002725"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5532724.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3123..3422)
                     /locus_tag="OO179_002726"
     CDS             complement(3123..3422)
                     /locus_tag="OO179_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5532725.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3597..4592
                     /gene="wecH"
                     /locus_tag="OO179_002727"
     CDS             3597..4592
                     /gene="wecH"
                     /locus_tag="OO179_002727"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5532726.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4634..5071)
                     /locus_tag="OO179_002728"
     CDS             complement(4634..5071)
                     /locus_tag="OO179_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5532727.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5117..5458)
                     /locus_tag="OO179_002729"
     CDS             complement(5117..5458)
                     /locus_tag="OO179_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532728.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="OO179_002730"
     CDS             complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="OO179_002730"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5532729.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="OO179_002731"
                     /pseudo
     CDS             complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="OO179_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8841..9833
                     /gene="xylF"
                     /locus_tag="OO179_002732"
     CDS             8841..9833
                     /gene="xylF"
                     /locus_tag="OO179_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5532730.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9911..11452
                     /gene="xylG"
                     /locus_tag="OO179_002733"
     CDS             9911..11452
                     /gene="xylG"
                     /locus_tag="OO179_002733"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5532731.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSKHHV
                     EKQSV"
     gene            11430..12611
                     /gene="xylH"
                     /locus_tag="OO179_002734"
     CDS             11430..12611
                     /gene="xylH"
                     /locus_tag="OO179_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5532732.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12689..13866
                     /gene="xylR"
                     /locus_tag="OO179_002735"
                     /pseudo
     CDS             12689..13866
                     /gene="xylR"
                     /locus_tag="OO179_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13974..14798)
                     /locus_tag="OO179_002736"
     CDS             complement(13974..14798)
                     /locus_tag="OO179_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5532733.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="OO179_002737"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="OO179_002737"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5532734.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="OO179_002738"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="OO179_002738"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5532735.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="OO179_002739"
     CDS             complement(18730..19203)
                     /locus_tag="OO179_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5532736.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="OO179_002740"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="OO179_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5532737.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20354..21352
                     /gene="yiaK"
                     /locus_tag="OO179_002741"
     CDS             20354..21352
                     /gene="yiaK"
                     /locus_tag="OO179_002741"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5532738.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21364..>21504
                     /locus_tag="OO179_002742"
     CDS             21364..>21504
                     /locus_tag="OO179_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5532739.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5400 a         5487 c         5282 g         5335 t
ORIGIN      
        1 agcctgccag atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg
       61 caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt
      121 ctttgtcatc aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct
      181 ctgccagttc gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt
      241 cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg
      301 cattcacgcg atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt
      361 tcgccgccgc cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc
      421 gggcatcgaa atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa
      481 cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat
      541 catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca
      601 gcgtttgcag atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg
      661 cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac
      721 ccgccagagt atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca
      781 ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg
      841 cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga
      901 agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa
      961 cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt
     1021 cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt
     1081 ccagacgttt tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa
     1141 cgactttctc gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga
     1201 agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac
     1261 ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg
     1321 cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta
     1381 agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt
     1441 tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat
     1501 cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat
     1561 cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg
     1621 tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg
     1681 gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct
     1741 tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt
     1801 caacggtaat accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag
     1861 cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt
     1921 ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc
     1981 cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca
     2041 ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct
     2101 cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg
     2161 aagcgtaata tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct
     2221 gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt
     2281 tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg
     2341 cttctttctc gtactgctca aagcaggtga acaggaagtc cacatccgcg tattcgaagt
     2401 tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca
     2461 gcgggccgtc gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca
     2521 gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct
     2581 gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc
     2641 cccaggcccc cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag
     2701 tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg
     2761 atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt
     2821 agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc
     2881 ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg
     2941 gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct
     3001 ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa
     3061 ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg
     3121 gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc
     3181 tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg
     3241 acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct
     3301 tccatattca acgaacgcag aggatcaacc tttacccgtt gtgctttctg cacgggtgac
     3361 ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc
     3421 attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat
     3481 atcttacgaa tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt
     3541 gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc
     3601 agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga
     3661 ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata
     3721 tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg
     3781 gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat
     3841 gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata
     3901 tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct
     3961 tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa
     4021 aaatgttgct ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc
     4081 agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt
     4141 actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag
     4201 cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga
     4261 cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc
     4321 cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc
     4381 gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg
     4441 ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg
     4501 atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac
     4561 aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc
     4621 gggcttacgt gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt
     4681 ttattccggc atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga
     4741 aagccagtcc ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca
     4801 tcagtagagc aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg
     4861 tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc
     4921 ctaacaccag tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata
     4981 gccctaacag gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg
     5041 ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt
     5101 aagagcttta tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa
     5161 aaaaggcgat ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga
     5221 taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat
     5281 ccagttgccc gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta
     5341 acacgccaag aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca
     5401 gataaaccag cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa
     5461 cgatctccat atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaaaaa
     5521 tgccattaac aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat
     5581 atatagatgt gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg
     5641 gcagaagttg ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac
     5701 gctgcgcatc tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg
     5761 atttctctgg attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat
     5821 ccccccccgt acggtaatcg agctgctgac cgctgatatc cgccagcatc tgacgccagt
     5881 actcactacg cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat
     5941 gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca
     6001 gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat
     6061 tgtgtggtgt acgctcgccg gaaagataag gcagaaacca aactggctcg gcactttcat
     6121 cagcctgttg agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg
     6181 cccaatccag acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta
     6241 gcgcatggca aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag
     6301 caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca accattccca
     6361 caccaactgc accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac
     6421 cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg
     6481 cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct
     6541 ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg
     6601 tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct
     6661 ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt
     6721 tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac
     6781 agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg
     6841 ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat
     6901 cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg
     6961 accaaagtgg atgcgggcgc gaaacggtca gcttttccgt ttgcgaagca accacctcac
     7021 cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca
     7081 tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg
     7141 cacagttagc cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg
     7201 gcgaccactc tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc
     7261 cagtgacatt tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg
     7321 ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc
     7381 cagtgccatc gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact
     7441 ttgacgacgt actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag
     7501 aatttcatac atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca
     7561 gcccagttgc gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc
     7621 ggtggctatt tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt
     7681 cagtttaatc tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc
     7741 gtaatcatat tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgcccta
     7801 gaagccgatt ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg
     7861 ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac
     7921 atagttttca ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc
     7981 ccagctgaag acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt
     8041 aaagcagtta gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag
     8101 gacatcaacc atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga
     8161 aacatccaca tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc
     8221 gacatctgct ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt
     8281 aaacgccccc acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc
     8341 ggcaaaacgc aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg
     8401 acggaatgct aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc
     8461 aaaataggct tgcatattga actccataat caggtaatgc cgcgggtgat ggatgatgtc
     8521 gtaatattgg gcactccctt tcaattgctc aattatgtta tttcacactg ctattgagat
     8581 aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat gatgaaactg ggtaattcct
     8641 cgaagataaa aatgcaataa gtacaattgc gcaacaaaag taagatctcg gtcataaatc
     8701 aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt tcccctgttt
     8761 agttgctaaa aattggttac gtttatcgcg gtgattgtta cttattaaag ctgtcctcta
     8821 actacagaag gccctacacc atgaaaataa agaacattct actcaccctt tgcacctcac
     8881 ttctgcttac caacgttgcg gcacacgcca aagaagtcaa aataggtatg gcgattgatg
     8941 atctccgtct tgaacgctgg caaaaagatc gagatatttt tgtgaaaaag gcagaatctc
     9001 tcggtgcgaa agtatttgta cagtctgcaa atggcaatga agaaacacaa atgtcgcaga
     9061 ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg
     9121 tattaagtaa cgttgtaaaa aaagccaaac aagaaggtat taaagtatta gcttacgacc
     9181 gtatgattaa cgatgcggat atcgattttt atatttcttt cgataacgaa aaagtcggtg
     9241 aactgcaggc aaaagccttg gtcgatattg ttccgcaagg taattacttc ctgatgggcg
     9301 gctcgccggt agataacaac gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac
     9361 cttacgttga ttctggaaaa attaaagtcg ttggtgacca atgggttgat ggctggttac
     9421 cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc caataataac aaaattgatg
     9481 ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat tcaggcatta agcgcgcaag
     9541 gtttatcagg gaaagtagca atttccggcc aggatgcgga tctcgcaggt attaaacgta
     9601 ttgctgccgg tacgcaaact atgacggtgt ataaacctat tacattgctg gcaaatactg
     9661 ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc aaaagcagat acctcactga
     9721 ataatggcct gaaagatgtc ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca
     9781 acatcaaaga tacggtaatt aaagacggat tccacaaaga gagcgagctg taagcgttac
     9841 gccccggcgc ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg
     9901 tggagtcgtt atgtcttatc tacttgaaat gaagaacatt accaaaacct tcggcagtgt
     9961 gaaggcgatt gataacgtca gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg
    10021 ggaaaatggg tctggtaaat caacgctgat gaaagtgctg tgtggtattt atccccatgg
    10081 ctcctacgaa ggcgaaatta tttttgcggg agaagagatt caggcgagtc acatccgcga
    10141 taccgaacgc aaaggtatcg ccatcattca ccaggaattg gccctggtga aagaattgac
    10201 cgtgctggaa aatatcttcc tgggtaacga aataacccac aatggcatta tggattatga
    10261 cctgatgacg ctacgctatc agaagctgct cgcacaggtc agtttatcca tttcacctga
    10321 tacccgcgtt ggcgatttag ggcttgggca acaacaactg gttgaaattg ccaaggcact
    10381 taataaacag gtgcgcttgt taattctcga tgaaccgaca gcctcattaa ctgagcagga
    10441 aacgtcggtt ttactggata ttattcgcga tctacaacag cacggtatcg cctgtattta
    10501 tatttcgcac aaactcaacg aagtcaaagc gatttccgat acgatttgcg ttattcgcga
    10561 cggacagcac attggtacgc gtgatgctgc cggaatgagt gaagacgata ttatcaccat
    10621 gatggtcggg cgagagttaa ccgcgattta ccctaatgaa ccacatacca ccggagatga
    10681 aatattacgt attgaacatc tgacggcatg gcatccggtc aatcgtcata ttaaacgagt
    10741 taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg gactcgttgg
    10801 tgccggacgt accgagacca ttcagtgcct gtttggcgtg tggcccggac aatgggaagg
    10861 aaaaatttat attgatggca aacaggtaga tattcgtaac tgtcagcaag ccatcgccca
    10921 ggggattgcg atggtacccg aagacagaaa gcgcgacggc atcgttccgg taatggcggt
    10981 tggtaaaaat attaccctcg ccgcactcaa taaatttacc ggtggcatta gccagcttga
    11041 tgacgcggca gagcaaaaat gtattctgga atcaattcag caactcaaag ttaaaacgtc
    11101 gtcccccgac cttgctattg gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc
    11161 ccgctgtctg ttacttaacc cgcgcatcct cattcttgat gaacctacca ggggtattga
    11221 tattggcgcg aaatatgaga tctacaaatt aattaaccaa ctcgtccagc agggtattgc
    11281 cgttattgtc atctcttccg aattacctga agtgctcggc cttagcgatc gtgtactggt
    11341 gatgcatgaa gggaaactaa aagccaacct gataaatcat aacctgactc aggagcaggt
    11401 gatggaagcc gcattgagga gcaaacatca tgtcgaaaag caatccgtct gaagtgaaat
    11461 tggccgtacc gacatccggt ggcttttctg ggctgaaatc actgaatttg caggtcttcg
    11521 tgatgattgc agctatcatc gcaatcatgc tgttctttac ctggaccacc gatggtgcct
    11581 acttaagcgc ccgtaacgtc tccaacctgt tacgccagac cgcgattacc ggcatcctcg
    11641 cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc ggctcaatga
    11701 tggggctatt aggtggcgtc gcggcgattt gtgacgtctg gttaggctgg cctttgccac
    11761 ttaccatcat tgtgacgttg gttctgggac tgcttctcgg tgcctggaac ggatggtggg
    11821 tcgcgtaccg caaagtccct tcatttattg tcaccctcgc gggcatgttg gcatttcgcg
    11881 gcatactcat tggcatcacc aacggcacga ctgtatcccc caccagcgcc gcgatgtcac
    11941 aaattgggca aagctatctc cccgccagta ccggcttcat cattggcgcg cttggcttaa
    12001 tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt
    12061 ctccggcctc taccgcagta gtcggtcgcc aagctttaac cgctatcatc gtattaggcg
    12121 caatctggct gttgaatgat taccgtggcg ttcccactcc tgttctgctg ctgacgttgc
    12181 tgttactcgg cggaatgttt atggcaacgc ggacggcatt tggacgacgc atttatgcca
    12241 tcggcggcaa tctggaagca gcacgtctct ccgggattaa cgttgaacgc accaaacttg
    12301 ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg attaatcctt agttctcgac
    12361 ttggcgctgg ttcaccttct gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg
    12421 tgattggcgg caccagcctg gctggcggtg tgggaagcgt tgccggagca gtaatggggg
    12481 catttatcat ggcttcactg gataacggca tgagtatgat ggatgtaccg accttctggc
    12541 agtatatcgt taaaggtgcg attctgttgc tggcagtatg gatggactcc gcaaccaaac
    12601 gccgttcttg attttgataa aaattttctc aaagccggtt acgtattacc ggttttgagt
    12661 ttttgcatga ttcagcagga aaagaaccat gtttactaaa cgtcaccgca tcacattact
    12721 gttcaatgcc aataaagcct atgaccggca ggtagtagaa ggcgtagggg aatatttaca
    12781 ggcatcacaa tcagaatggg atattttcat tgaagaagat ttccgcgccc gcattgataa
    12841 aatcaaggac tggttaggag atgtcgtcat tgccgacttc gacgacaaac agatcgagca
    12901 agcgctggct gatgtcgacg tccccattgt tggggttggc ggctcgtatc accttgcaga
    12961 aagttaccca cccgttcatt acattgccac cgataactat gcgctggttg aaagcgcatt
    13021 tttgcattta aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacata tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgaccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg ggcaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg acgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacgcctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggcaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgttgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgctc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaac
    20341 aaggaagcct aggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
    20401 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
    20461 caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
    20521 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
    20581 tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg
    20641 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca
    20701 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
    20761 ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcatcggcac
    20821 taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc
    20881 gatgttctct tacggcatgt tagaagttaa ccgcctggca ggccgtcagc tcccggtcga
    20941 tggtggcttt gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaagaatcg
    21001 ccgcattttg ccgatgggct actggaaagg ttctggcatg tcgattgtgc tggatatgat
    21061 cgctactctc ctttccgacg gcgcatcggt tgccgaagtc acccaggaca acagcgacga
    21121 atacggcatt tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac
    21181 ccgcgatgcc aagctgcaac gtatcatgga ttacgttact accgctgaac gcgctgacga
    21241 aaaccaggcc atccgcttac ccggccacga atttactacc ctgctggccg aaaaccgccg
    21301 taacggcatc actgttgatg acagcgtgtg ggccaaaatc caggcgttat gaggagataa
    21361 gtcatgattt ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc cgccattgaa
    21421 aaggcgctcg attttctgcg cgctaccaac ttcaacgtcc tggagccggg cgttgtcgaa
    21481 atcgacggca agaatattta tgcg
//
LOCUS       DAJTNW010000069        21427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNW010000069 DAJTNW010000000
VERSION     DAJTNW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..447)
                     /locus_tag="OO179_002743"
     CDS             complement(<1..447)
                     /locus_tag="OO179_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5532740.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO179_002744"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO179_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5532741.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO179_002745"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO179_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5532742.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO179_002746"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO179_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5532743.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO179_002747"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO179_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5532744.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO179_002748"
     CDS             complement(5217..6815)
                     /locus_tag="OO179_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5532745.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO179_002749"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO179_002749"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5532746.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO179_002750"
     CDS             complement(8494..9072)
                     /locus_tag="OO179_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5532747.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO179_002751"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO179_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5532748.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO179_002752"
     CDS             10511..10690
                     /locus_tag="OO179_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5532749.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11169)
                     /locus_tag="OO179_002753"
     CDS             complement(10810..11169)
                     /locus_tag="OO179_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5532750.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="OO179_002754"
     CDS             complement(11531..11875)
                     /locus_tag="OO179_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5532751.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="OO179_002755"
     CDS             12007..13917
                     /locus_tag="OO179_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5532752.1"
                     /translation="MTDDFAPDGQLAKAIPGLKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="OO179_002756"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="OO179_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5532753.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="OO179_002757"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="OO179_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5532754.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="OO179_002758"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="OO179_002758"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5532755.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="OO179_002759"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="OO179_002759"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5532756.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO179_002760"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO179_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5532757.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO179_002761"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO179_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="OO179_002762"
     CDS             complement(20412..20978)
                     /locus_tag="OO179_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5532758.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..21288
                     /locus_tag="OO179_002763"
     CDS             21190..21288
                     /locus_tag="OO179_002763"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5532759.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREVR"
     gene            21345..>21427
                     /locus_tag="OO179_002764"
     CDS             21345..>21427
                     /locus_tag="OO179_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5532760.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSI"
BASE COUNT         5321 a         5305 c         5500 g         5301 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacaacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc accattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tattgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatcct tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg ttcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcctt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg acgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaaa aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacagc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacgctctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggggatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgag catagctctt gaattgagca tttttctgcg
    21121 gtatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagagagg tgaggtagcc tgagtttaac
    21301 ggacactcct tcctgaaata gaatggcatc agaaggagct aataatgagc agaaaaaccc
    21361 aacgttactc taaagagttc aaagccgaag ctgtcagaac ggttcttgaa aatcaacttt
    21421 cgatcag
//
LOCUS       DAJTNW010000070        21387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNW010000070 DAJTNW010000000
VERSION     DAJTNW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..134)
                     /locus_tag="OO179_002765"
     CDS             complement(<1..134)
                     /locus_tag="OO179_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532761.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="OO179_002766"
     CDS             complement(236..1216)
                     /locus_tag="OO179_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5532762.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="OO179_002767"
     CDS             complement(1224..1934)
                     /locus_tag="OO179_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5532763.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO179_002768"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO179_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5532764.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="OO179_002769"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="OO179_002769"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5532765.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="OO179_002770"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="OO179_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5532766.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="OO179_002771"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="OO179_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5532767.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="OO179_002772"
     CDS             8511..8621
                     /locus_tag="OO179_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5532768.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO179_002773"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO179_002773"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5532769.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO179_002774"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO179_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5532770.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="OO179_002775"
     CDS             11503..12135
                     /locus_tag="OO179_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5532771.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO179_002776"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO179_002776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5532772.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="OO179_002777"
     CDS             15667..16599
                     /locus_tag="OO179_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5532773.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="OO179_002778"
     CDS             complement(16640..18070)
                     /locus_tag="OO179_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5532774.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO179_002779"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO179_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5532775.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO179_002780"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO179_002780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5532776.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="OO179_002781"
     CDS             complement(19931..20200)
                     /locus_tag="OO179_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5532777.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="OO179_002782"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="OO179_002782"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5532778.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="OO179_002783"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="OO179_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5532779.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAJTNW010000071        21254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNW010000071 DAJTNW010000000
VERSION     DAJTNW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..792
                     /locus_tag="OO179_002784"
     CDS             <1..792
                     /locus_tag="OO179_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5532780.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(852..2150)
                     /gene="eno"
                     /locus_tag="OO179_002785"
     CDS             complement(852..2150)
                     /gene="eno"
                     /locus_tag="OO179_002785"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5532781.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="OO179_002786"
     CDS             complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="OO179_002786"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5532782.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="OO179_002787"
     CDS             complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="OO179_002787"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5532783.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4990..7224)
                     /locus_tag="OO179_002788"
     CDS             complement(4990..7224)
                     /locus_tag="OO179_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5532784.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="OO179_002789"
     CDS             complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="OO179_002789"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5532785.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8630..11386
                     /gene="barA"
                     /locus_tag="OO179_002790"
     CDS             8630..11386
                     /gene="barA"
                     /locus_tag="OO179_002790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5532786.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="OO179_002791"
     CDS             complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="OO179_002791"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5532787.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12979..14319)
                     /locus_tag="OO179_002792"
     CDS             complement(12979..14319)
                     /locus_tag="OO179_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5532788.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="OO179_002793"
     CDS             complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="OO179_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5532789.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16107..16556)
                     /locus_tag="OO179_002794"
     CDS             complement(16107..16556)
                     /locus_tag="OO179_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5532790.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16574..17356)
                     /gene="truC"
                     /locus_tag="OO179_002795"
     CDS             complement(16574..17356)
                     /gene="truC"
                     /locus_tag="OO179_002795"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5532791.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17356..17685)
                     /locus_tag="OO179_002796"
     CDS             complement(17356..17685)
                     /locus_tag="OO179_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5532792.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18307..18852)
                     /gene="syd"
                     /locus_tag="OO179_002797"
     CDS             complement(18307..18852)
                     /gene="syd"
                     /locus_tag="OO179_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5532793.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18920..19768
                     /gene="queF"
                     /locus_tag="OO179_002798"
     CDS             18920..19768
                     /gene="queF"
                     /locus_tag="OO179_002798"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5532794.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19880..21244
                     /gene="ppnN"
                     /locus_tag="OO179_002799"
                     /gene_synonym="ygdH"
     CDS             19880..21244
                     /gene="ppnN"
                     /locus_tag="OO179_002799"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5532795.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5227 a         5670 c         5072 g         5285 t
ORIGIN      
        1 gttgcagccc aaccaactat tgtcccacaa ttacagcaac aagttacggg tcttacgagt
       61 agcttaaact cacaagaaaa gaaagaactg acgcacaagt tagaatctat tttcaataat
      121 acccaagtgc aaattgctgt attaattgtt tccacaacca aagatgagac aattgaacaa
      181 tatgctacaa gagtttttga caattggcgt ttaggagatg ccaaacgtaa tgatgggata
      241 ctgatcgttg ttgcctggtc ggatcgcact gtccgcatcc aggtaggtta tgggctggaa
      301 gaaaaggtaa ccgatgctct ggctggagat atcatccgta gcaacatgat acccgcattt
      361 aaacaacaaa aattagctaa gggattagag ttagctataa tcgctttgaa taaccaactc
      421 acttcacaac accaatatcc ggctaatcct tcagaaagtg aatcaacgtc ttccactgat
      481 cattattact ttgctatttt ttgggtattt gcagtgatgt tcttcccttt ctggtttttt
      541 catcaaggca gtaatttttg tcgcgcatgt aaaagtggag tctgtatttc agcaatccat
      601 cttttagatt tattcctgtt ctccgataaa actttttcca ttgctgtatt ttccttcttt
      661 tttactttca ccatatttat ggtctttacc tgtttatgtg tacttcagaa aagagcatct
      721 ggtagaagtt atcattcaga caacagcggt tccgctggag gctcaagtgg cggcgcatcc
      781 ggccgctggt aactccacca taaaaaatgc cagcccgaag gctggcatta tcaaatcaga
      841 taaagtcaga cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg
      901 cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc
      961 atagaaccag ttttgatctg gcctgcagca gtaccaacag ccagatcagc gatggtagca
     1021 tcttcagttt caccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc
     1081 ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag
     1141 ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg
     1201 tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag
     1261 tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc
     1321 aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat
     1381 ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc
     1441 agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa
     1501 cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc
     1561 agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt
     1621 ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca
     1681 ccaccgttga tgatgttcat catcggaacc ggcatggagt atttgcccgg agtgccgttc
     1741 agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta
     1801 gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc
     1861 aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc
     1921 tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg
     1981 gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga
     2041 gcagctgcca tgccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta
     2101 ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc
     2161 tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaagg tacgcgttgc
     2221 cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca
     2281 aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg
     2341 caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg
     2401 gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact
     2461 tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtatagctg gcgaaccagg
     2521 ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc
     2581 ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca
     2641 accgggtact tacagtctgg cacaaattct gtagagttgg cgttctccat gttggcaaca
     2701 tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata
     2761 ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa
     2821 ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca
     2881 tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc
     2941 agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg
     3001 atggtgactt cacttaccgg attcgcttct tcgaagataa cctgttccca ttcggacaga
     3061 tttgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat
     3121 ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt
     3181 tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggtac agcgcgatct
     3241 gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc
     3301 tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc
     3361 ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatagc ttcgaggaac
     3421 ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct
     3481 tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg
     3541 gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc
     3601 gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg
     3661 tccaagtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc
     3721 atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac attgaggcca
     3781 cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg
     3841 accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa
     3901 aagacgatgg aataaccaag acgggaaagc agtatacccg aacatgacct gtgccacaaa
     3961 ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc
     4021 ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat
     4081 ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga
     4141 cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac
     4201 gctccacttc gcgaaaacga cgctcgaatt tttcgttcac tttttgcaat gcgatttctg
     4261 ctttcgtccc taaatggcga gccagattaa ccgtggcaaa cagcaggtcc cccatttcct
     4321 cctccagttt agcctggtcg acaacagcct gccgcgcctc gtacatcacc tcgtcgatct
     4381 cttcgtagac tttatcaacc accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc
     4441 aacgtttctg gattttttgc gcccgcatta aagccggtaa actacgagga atatcgtcca
     4501 gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa
     4561 gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta
     4621 atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca
     4681 tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa
     4741 aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa
     4801 tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct
     4861 gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct
     4921 gtcaggtgta ataccttcga gtaaaagata tcgctggata cggctgacgc catatccagc
     4981 tatgtaagac taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     5041 tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     5101 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     5161 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5221 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5281 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5341 tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5401 gtggtgcatc aggttaccaa caccttcaac caccacacga ccgttatctt tactgcggtt
     5461 ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc
     5521 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5581 gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg gcagcagatg
     5641 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5701 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     5761 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5821 ctgtttctgg gtgataattt caatctggtc gcccatttgc agctggtagg tgaacggcac
     5881 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5941 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     6001 gacatacacc cggtcgtcaa acacctgact acgtacttcg tcgagcattt cgccggaatc
     6061 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     6121 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6181 ctccgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt ttccacccgg
     6241 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacgtaatc
     6301 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     6361 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     6421 gaggttcttt ttctgcattt tgcgccagat gctgtagatg tgtttcggac gaccatacac
     6481 ttcagcttta acgccttcag ctttcatctc agcgcgcaga tggccgacaa actcttcgat
     6541 atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt
     6601 cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     6661 acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta cacgttcatc
     6721 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6781 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6841 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6901 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6961 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     7021 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     7081 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     7141 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7201 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7261 gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7321 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7381 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7441 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7501 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7561 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgca
     7621 ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7681 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7741 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7801 aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7861 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7921 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7981 gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct gcccagacat
     8041 gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca ttgtttgacg
     8101 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     8161 aagtagttca gacttaaacg cgcgcggcgg cgatagcccc agggaacatc ggcgatcact
     8221 tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8281 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8341 gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8401 ttatcttcag taacagcaac ttccgcgttt tcctgcggca ataatccggg gataaataac
     8461 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8521 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8581 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8641 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8701 gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8761 cggtgctagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8821 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8881 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8941 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     9001 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     9061 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgcttgagct
     9121 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcct gcgtgatgat
     9181 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9241 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9301 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9361 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9421 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc tggcgaaaaa
     9481 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9541 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9601 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9661 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9721 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9781 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9841 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9901 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9961 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
    10021 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccgggct
    10081 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
    10141 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10201 tattatcgaa gggccatcca tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc
    10261 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10321 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10381 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10441 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagttaatg ctgaaaaact
    10501 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10561 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10621 taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga aacttatcgg
    10681 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcagt
    10741 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgccggacat
    10801 ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcgac aaacgccggt
    10861 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10921 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10981 caagcctggc agcggtattt cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    11041 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    11101 tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    11161 tgaggaacag ctggctggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11221 tggcagttgc ggctatagcg gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca
    11281 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11341 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11401 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11461 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11521 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11581 aaccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11641 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11701 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11761 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    11821 tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca gtaattttac
    11881 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11941 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    12001 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    12061 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    12121 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    12181 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12241 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12301 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12361 catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    12421 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12481 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12541 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12601 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12661 cgcgcccact agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12721 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12781 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12841 tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatgac
    12901 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    12961 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    13021 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt
    13081 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    13141 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13201 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13261 cacatgggta aatatcgcca gagagatatc gaaatggttg ttggaatggc agccccaggt
    13321 cagcccccag tcgtcgcaaa gctgcgccac gcggactgca ccggaaagcg tccagaagtg
    13381 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13441 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgtcgaa actccgccat
    13501 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13561 atcattcagc cctttgcata aagaaatggc ttcatcaagc agccatgcac cgttgggatc
    13621 aacggtaatc cgcgcatccg ggaagctttt cttcaatgca cgaacagtgt cgatttcttg
    13681 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13741 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13801 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13861 tatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13921 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13981 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    14041 ggctgcctga ttacctttat gtacctgctg aaccacttta ttcaggcgcg caacttcctg
    14101 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    14161 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acgatattgc gggtgaagta
    14221 tgcgttatgc gcaccaccaa tattgagcaa catgctgtca tgtccggcga ccggaatgac
    14281 tttcatatca gtaataacag gactggattg tgtcgccatc ttattgcccc gcgacaggtt
    14341 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaggg
    14401 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    14461 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14521 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14581 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14641 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14701 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataattcca cccagcacac
    14761 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14821 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14881 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga
    14941 tctgccccca cttcacgctg aacggcactt taactttggt gctttgctga tccatattga
    15001 tcagcgcgcc acccgcggcg atatactcca gctctttctg atttaccccc ggatgttgat
    15061 ttggctcgtg gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa
    15121 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15181 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgca
    15241 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15301 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15361 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15421 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15481 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15541 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15601 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15661 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15721 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15781 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15841 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15901 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg
    15961 aagaaataaa gaagagtatg aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc
    16021 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    16081 aacgacattt accgctcgct gacatatcag gacaacagcg tgccccactg ttcgacccac
    16141 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16201 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa
    16261 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16321 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16381 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    16441 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16501 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16561 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16621 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16681 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16741 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16801 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatatc
    16861 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16921 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16981 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    17041 ccaccagtgg ataatccaac accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    17101 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    17161 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17221 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17281 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17341 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17401 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17461 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17521 cagcccccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17581 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17641 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17701 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17761 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17821 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17881 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17941 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    18001 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    18061 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    18121 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18181 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18241 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    18301 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18361 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18421 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18481 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18541 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18601 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18661 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18721 agttgaaata atgcacggtg aaggaacacc gtacaattcc tcacttaacg gccagctttt
    18781 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18841 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18901 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18961 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    19021 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    19081 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    19141 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19201 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19261 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19321 tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg atgaccagga
    19381 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    19441 aaaagtagtg gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19501 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19561 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19621 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
    19681 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19741 atcgaccaca agactagttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19801 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19861 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19921 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19981 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    20041 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    20101 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    20161 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccataatac
    20221 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20281 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    20341 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct
    20401 gggcctgcgt gagctgaata tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat
    20461 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    20521 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20581 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20641 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20701 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20761 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
    20821 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20881 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
    20941 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    21001 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    21061 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    21121 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21181 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21241 gtaatctccc ctcc
//
LOCUS       DAJTNW010000072        20836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNW010000072 DAJTNW010000000
VERSION     DAJTNW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO179_002800"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO179_002800"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5532796.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO179_002801"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO179_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5532797.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO179_002802"
     CDS             complement(2649..3200)
                     /locus_tag="OO179_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5532798.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO179_002803"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO179_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5532799.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OO179_002804"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OO179_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5532800.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO179_002805"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO179_002805"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5532801.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO179_002806"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO179_002806"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5532802.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO179_002807"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO179_002807"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5532803.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO179_002808"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO179_002808"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5532804.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO179_002809"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO179_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5532805.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO179_002810"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO179_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5532806.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO179_002811"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO179_002811"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5532807.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO179_002812"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO179_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5532808.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO179_002813"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO179_002813"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5532809.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO179_002814"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO179_002814"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5532810.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO179_002815"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO179_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5532811.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO179_002816"
     CDS             complement(18174..18569)
                     /locus_tag="OO179_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5532812.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO179_002817"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO179_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5532813.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO179_002818"
     CDS             19485..20078
                     /locus_tag="OO179_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5532814.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OO179_002819"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OO179_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5532815.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4962 a         5226 c         5520 g         5128 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatt
//
LOCUS       DAJTNW010000073        20713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNW010000073 DAJTNW010000000
VERSION     DAJTNW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO179_002820"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO179_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5532816.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO179_002821"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO179_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5532817.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO179_002822"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO179_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5532818.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO179_002823"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO179_002823"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5532819.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO179_002824"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO179_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5532820.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO179_002825"
     CDS             complement(8217..9110)
                     /locus_tag="OO179_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5532821.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO179_002826"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO179_002826"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5532822.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO179_002827"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO179_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5532823.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO179_002828"
     CDS             complement(10241..11473)
                     /locus_tag="OO179_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5532824.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO179_002829"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO179_002829"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5532825.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO179_002830"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO179_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5532826.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO179_002831"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO179_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO179_002832"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO179_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5532827.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO179_002833"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO179_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5532828.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO179_002834"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO179_002834"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5532829.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO179_002835"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO179_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5532830.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4983 a         5725 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAJTNW010000074        20650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNW010000074 DAJTNW010000000
VERSION     DAJTNW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            134..433
                     /locus_tag="OO179_002836"
     CDS             134..433
                     /locus_tag="OO179_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5532831.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            443..967
                     /gene="ygaP"
                     /locus_tag="OO179_002837"
     CDS             443..967
                     /gene="ygaP"
                     /locus_tag="OO179_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5532832.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="OO179_002838"
     CDS             complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="OO179_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5532833.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2086..2535
                     /gene="alaE"
                     /locus_tag="OO179_002839"
     CDS             2086..2535
                     /gene="alaE"
                     /locus_tag="OO179_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5532834.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2572..2916)
                     /locus_tag="OO179_002840"
     CDS             complement(2572..2916)
                     /locus_tag="OO179_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5532835.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3068..3298
                     /locus_tag="OO179_002841"
     CDS             3068..3298
                     /locus_tag="OO179_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532836.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3645..3890
                     /gene="nrdH"
                     /locus_tag="OO179_002842"
     CDS             3645..3890
                     /gene="nrdH"
                     /locus_tag="OO179_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5532837.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3887..4297
                     /gene="nrdI"
                     /locus_tag="OO179_002843"
     CDS             3887..4297
                     /gene="nrdI"
                     /locus_tag="OO179_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5532838.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4270..6414
                     /gene="nrdE"
                     /locus_tag="OO179_002844"
     CDS             4270..6414
                     /gene="nrdE"
                     /locus_tag="OO179_002844"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5532839.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6424..7383
                     /gene="nrdF"
                     /locus_tag="OO179_002845"
     CDS             6424..7383
                     /gene="nrdF"
                     /locus_tag="OO179_002845"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5532840.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7738..8940
                     /gene="proV"
                     /locus_tag="OO179_002846"
     CDS             7738..8940
                     /gene="proV"
                     /locus_tag="OO179_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5532841.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8933..9997
                     /gene="proW"
                     /locus_tag="OO179_002847"
     CDS             8933..9997
                     /gene="proW"
                     /locus_tag="OO179_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5532842.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10055..11047
                     /gene="proX"
                     /locus_tag="OO179_002848"
     CDS             10055..11047
                     /gene="proX"
                     /locus_tag="OO179_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5532843.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11239..12418
                     /locus_tag="OO179_002849"
                     /pseudo
     CDS             11239..12418
                     /locus_tag="OO179_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12542..13279
                     /gene="ygaZ"
                     /locus_tag="OO179_002850"
     CDS             12542..13279
                     /gene="ygaZ"
                     /locus_tag="OO179_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5532844.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13269..13604
                     /gene="ygaH"
                     /locus_tag="OO179_002851"
     CDS             13269..13604
                     /gene="ygaH"
                     /locus_tag="OO179_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5532845.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13695..14225
                     /gene="emrR"
                     /locus_tag="OO179_002852"
     CDS             13695..14225
                     /gene="emrR"
                     /locus_tag="OO179_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5532846.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14352..15524
                     /gene="emrA"
                     /locus_tag="OO179_002853"
                     /pseudo
     CDS             14352..15524
                     /gene="emrA"
                     /locus_tag="OO179_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15541..17079
                     /gene="emrB"
                     /locus_tag="OO179_002854"
     CDS             15541..17079
                     /gene="emrB"
                     /locus_tag="OO179_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5532847.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="OO179_002855"
     CDS             complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="OO179_002855"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5532848.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="OO179_002856"
     CDS             complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="OO179_002856"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5532849.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19437..19865)
                     /locus_tag="OO179_002857"
     CDS             complement(19437..19865)
                     /locus_tag="OO179_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5532850.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="OO179_002858"
     CDS             complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="OO179_002858"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5532851.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4976 a         5282 c         5163 g         5229 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctattcag cttacgcttt tactctcatt
       61 tacgctgctg aggctggtat atgttgcatt tttagtttag agtttactta atttagaaaa
      121 tacttaaata aatatgactg aactcgcgca attacaggcc agtaccgaac aggcagcggc
      181 cttattgaaa gcaatgagcc accctaaacg gttgctgatt ctgtgcatgc ttagcggttc
      241 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagcgcct ctgcgacatc
      301 acagcatctc gctcgtatgc gggacgaagg gcttatcaac agccaacggg atgcccaacg
      361 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
      421 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
      481 attaatcgca cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga
      541 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc
      601 taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
      661 cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
      721 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
      781 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
      841 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
      901 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
      961 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc
     1021 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
     1081 ccctgaccgg tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga
     1141 cgcggctggc gttttttacc agcacgtggt gcagcagcag agctattacc caataactct
     1201 tccgggttaa ttccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc
     1261 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
     1321 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg
     1381 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
     1441 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
     1501 tttaatctat ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag
     1561 aataatttca agattatata ttagccattt tgatgagtaa tttaggttat tggtgaacag
     1621 agtggcgctc tctgctcgct aactccgcaa gcctgacaaa accgaagtga ataaaaatgc
     1681 aatataacag aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt
     1741 tctggcatca aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt
     1801 atagcgttac ctccccccca aactttcatt agtgaaatta aaggcaagat catatctaat
     1861 tgatttaatt gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgag
     1921 ccgttacgga atattttatc tacaaaaact aactaaataa aaatttttca ctaattgatt
     1981 agtcatagac agcgatatac gctatgcaaa aatgcagatg gcaatgagat ccactgcttt
     2041 catctccatt aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag
     2101 tcacgcttgc gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg
     2161 aacatgtgta ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga
     2221 ttggtagcga ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg
     2281 tttatgcgcg cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc
     2341 ctggcttatg tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca
     2401 gactggcatc agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg
     2461 gcggtttatg gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag
     2521 caggtaaaag cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc
     2581 ttcgccaaat aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg
     2641 gataccgtaa tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc
     2701 gcaagtttta atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg
     2761 atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc
     2821 cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc
     2881 aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attttcaacc
     2941 gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc
     3001 gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg
     3061 ggatcatatg tataaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca
     3121 gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga
     3181 cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag
     3241 ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgagc
     3301 ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat
     3361 ttttatcggc gcactgttaa gcgtgcgcaa atcgtagtgc aaaaatgata ataaatacgc
     3421 gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc
     3481 ccctcccccg tcacgctcat atccagggta atttcgatca ctatttgcta tatattgtgt
     3541 ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
     3601 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc ataactattt
     3661 atactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct
     3721 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc
     3781 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
     3841 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
     3901 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     3961 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     4021 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     4081 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa
     4141 ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     4201 gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     4261 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     4321 gcgctgaatg cgatgcttaa tctttacgat agcgcaggtc gcattcagtt cgataaagac
     4381 cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt
     4441 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     4501 aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt
     4561 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     4621 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     4681 gcacaaggcg atgagacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt
     4741 cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
     4801 tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca
     4861 ctgcagctgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg
     4921 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
     4981 ttggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc aggtgcagtc
     5041 tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc
     5101 gacgaaaaaa tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga tatcactttc
     5161 catctggcaa aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt
     5221 tatggcaagc cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat
     5281 gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc
     5341 cagtttgagt ccggctatcc ctacatcatg tatgaagaca cagtaaaccg tgctagccct
     5401 atcgccgggc gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc
     5461 tcagagtatg acgagaatct cgactatgcc cgcacaggcc atgatatttc ctgcaattta
     5521 ggttcgttga atattgcgca caccatggat tcccccgatt tttcccgcac ggtagagact
     5581 gccgtgcgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc
     5641 gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt acatggttat
     5701 ctggcgaggg aaggcatcgc ttatggttcg ccggaagccc tggatttcac caatctctat
     5761 ttctatacca tcacctggca cgcactgcgt acctcgatgt tgctggcgcg cgaacgcggt
     5821 gaaaccttcg ccgggttcaa acagtcacgc tatgccagtg gtgaatattt cagccaatat
     5881 ctgcaaggta actggcagcc gaaaacggcg aaagttggcg aactgtttgc ccgcagctgt
     5941 attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc
     6001 atatacaacc agaatctcca ggcggtgccg ccaaccgggt ctatctctta tatcaaccat
     6061 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
     6121 ggacgcgttt actaccctgc cccgtttatg actaacgaaa atctggcgct gtatcaggac
     6181 gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc
     6241 gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac
     6301 aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt
     6361 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
     6421 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
     6481 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg
     6541 aacgatattc ccgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt
     6601 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg
     6661 cccgatgcgc tcacgcctca tgaagaggcg gtattatcga atatcagctt tatggaagcg
     6721 gttcatgccc gctcttacag ttcgattttc tcgacgctgt gccagaccaa agatgtcgat
     6781 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
     6841 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
     6901 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
     6961 aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
     7021 ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag
     7081 agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag
     7141 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacaccaat
     7201 aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat
     7261 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt
     7321 tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc
     7381 tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata
     7441 gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc
     7501 ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct
     7561 cgatttgctc tcagccctta tatcacggaa aattccggcg atttgctcgc atcaatattc
     7621 atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt
     7681 gactattccc attgggtaat atatcgacat aaacaaataa aggaatcatt ctattgcatg
     7741 gccattaaat taaaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagcg
     7801 ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac tgggctatcg
     7861 cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga
     7921 ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc
     7981 cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt
     8041 gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg
     8101 accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc cgaagaacgc
     8161 cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac
     8221 ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt
     8281 aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt aattcgcacc
     8341 gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt
     8401 tcccacgatc ttgatgaagc catgcgtatt ggcgaccgaa ttgccattat gcaaaatggt
     8461 gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc
     8521 cgtaccttct tccgtggcgt tgatattagt caggtgttca gtgcgaaaga tattgcccgc
     8581 cggacaccga atggcttaat tcgtaaaact cctggcttcg gcccacgttc ggcactgaaa
     8641 ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc
     8701 ggcgcagtct ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg
     8761 gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct
     8821 catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc
     8881 atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga
     8941 tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcgcaat ccgcagacgc
     9001 ctggggtaca ccgacgactg caccggctga cggcggtggt gctgactggc tgaccagtac
     9061 gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc
     9121 gctcgacagt tgggtcactg aagggatcga ctgggttgtt acccatttcc gtcccgtctt
     9181 ccagggcgtg cgcgttccgg ttgattatat cctcaacggt ttccagcaat tgctgctggg
     9241 tatgccagcg ccagtggcga ttatcgtttt cgctctcatc acctggcaga tttccggggt
     9301 cggaatgggc gtggcgacgc tggtctcgct gattgccatc ggcgcaatcg gtgcctggtc
     9361 gcaggcaatg gtgactctgg cgctggtgtt aactgccctg ctgttctgta tcgtcatcgg
     9421 tttgccgttg gggatatggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact
     9481 gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt
     9541 tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc cgccgattat
     9601 ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc
     9661 attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac
     9721 cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg tcattgcctc
     9781 gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat
     9841 ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgatcgcct
     9901 gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg
     9961 ccctgttggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtggcgcc
    10021 gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac
    10081 agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat
    10141 tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc tgctggtcag
    10201 tcgtgcgctg gagaaattag gttataccgt caacaaaccc agcgaagtgg attacaacgt
    10261 tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccact
    10321 ccatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt
    10381 tgttaacggc gcagcacagg gttacctgat cgataagaaa accgccgacc aatacaaaat
    10441 caccaacatc gcacaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga
    10501 cggtaaagcg gatttaaccg gttgtaaccc tggctggggc tgcgaaggtg cgatcaacca
    10561 ccagcttgcc gcgtatgaac tgaccaacac cgtgactcat aatcagggga actacgcagc
    10621 gatgatggcc gacaccatca gtcgctacaa agagggcaaa ccggtgtttt attacacctg
    10681 gacgccgtac tgggtgagta atgagctgaa gccagggaaa gatgtggtct ggttgcaggt
    10741 gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa
    10801 ttacggcttc ccggtcagca ccatgcatat cgttgccaac aaagcctgga ccgagaaaaa
    10861 tccggcggca gccaaactgt ttgccattat gcagttgcca gtggccgata ttaacgccca
    10921 gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc acgttgatgg
    10981 ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca
    11041 gaagtaattt ttattcgggc ggataaggcg ttttcgcctc atccgccgct ctgtacacaa
    11101 tgcctgatgc gacgctgacg cgtcctatca tgcctacaaa ccacatcgca catttcaaca
    11161 agctatcttt catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc
    11221 acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc gctgatcgtg
    11281 ctgatgtcta tcgccaccgg tctggcggta gccagtaact attacgccca gccattgctc
    11341 gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc
    11401 gcgcagttgg gctatgccgc aggtctactg tttcttgttc ccctcggtga tatgtttgaa
    11461 cgccgccgcc tgattgtctc gatgacctta ctggcagcgg gcggtatgtt aatcactgcc
    11521 agcagtcagt cactggcgat gatgatcctc ggtacggcat taaccggttt attctcagtc
    11581 gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa acgcggcaaa
    11641 gtggttggca ccattatgag cggtctgctg ttggggatat tgctggcacg aaccgttgcc
    11701 gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcttc ggtgttaatg
    11761 gcactgatgg cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc
    11821 aactacccac agttgttggg ttccgttttc agcatgttca tcagcgataa gatcctgcgc
    11881 acccgcgcgt tgctgggctg catgaccttt gccaacttca gcattctctg gacctcaatg
    11941 gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgacattgg tctgtttggg
    12001 cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgacaagggc
    12061 aaatcgcacc acaactttcg gtctgcttct gctgttactt tcatggctgg caatctggtt
    12121 tggtcacact tccgtactgg cgttgattat cggcattctg gtgctggacc tcaccgtaca
    12181 gggcgtgcat atcactaacc agacggtaat ttatcgaata catcctgatg cgcgtaatcg
    12241 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    12301 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    12361 cctggttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    12421 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    12481 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    12541 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    12601 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    12661 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    12721 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    12781 actgagcgtc atggcaatgg atgttcgcca tgttttgtat ggcccgtcac tgcgtagccg
    12841 tattattcag cgtctgcaaa aatcgaaaac cgccctatgg gcgtttggcc tgacggatga
    12901 ggtttttgcc gccgccaccg caaaactggt acgcaataat cgccgctgga gcgagaactg
    12961 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    13021 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat taggttttat
    13081 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    13141 cgttaccgca gctttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    13201 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    13261 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    13321 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    13381 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    13441 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    13501 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    13561 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    13621 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    13681 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    13741 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    13801 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    13861 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    13921 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    13981 aaaaacgcgg ttggatcgaa cgtcgtaaaa gcgataacga tcgccgctgc ctgcatctgc
    14041 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    14101 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    14161 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    14221 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    14281 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    14341 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    14401 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    14461 cgatagggat ttattggttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    14521 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    14581 ccgataacac cgattttgta aaagaaggcg atgttctggt cactctcgac ccgacagatg
    14641 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    14701 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg
    14761 cgaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    14821 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    14881 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    14941 ctgccgtgca acaggctgcc accgaagtac gtaacgccta gctggcgctg gagcgtaccc
    15001 gtattgtcag tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg ggtgcgcaaa
    15061 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    15121 actttaaaga gacgcagatt gccaatatgc gcatcggtca gccggtcacc atcaccaccg
    15181 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    15241 gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    15301 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    15361 ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    15421 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    15481 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    15541 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    15601 ctggcgacat tcatgcaggt gctgaactcc accattgcta acgtggcgat ccccactatc
    15661 gccgggaatc tgggctcatc gctcagccaa ggaacgtggg taatcacttc tttcggggtg
    15721 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    15781 ctgttccttt ggtccaccat cgcctttgct attgcgtctt gggcgtgtgg tgtctccagc
    15841 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    15901 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    15961 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    16021 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    16081 gtggtgttga tgacactgca aaccctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    16141 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    16201 gaccgcggta aagagctgga ctgtttttca tcacaggaaa ttatcattct taccgtggtg
    16261 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    16321 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    16381 tatatgctct acttcgacgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    16441 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgattctg
    16501 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    16561 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    16621 ttttgcgcgt cggcctggcc gcagtttatc caggggtttg cggtggtctg cttctttatg
    16681 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    16741 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    16801 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    16861 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    16921 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    16981 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    17041 ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt
    17101 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    17161 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacct cacgttccag
    17221 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    17281 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    17341 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    17401 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    17461 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    17521 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    17581 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    17641 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    17701 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    17761 tcagagatga aattttggcc actcccgagt ggcctttttc ttttctgtca ggcgtgtttt
    17821 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    17881 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    17941 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    18001 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    18061 tcatcacaca ctttctgata cgcttcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    18121 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    18181 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    18241 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    18301 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    18361 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    18421 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    18481 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    18541 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    18601 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    18661 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    18721 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    18781 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    18841 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    18901 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    18961 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    19021 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    19081 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    19141 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    19201 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    19261 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    19321 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    19381 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    19441 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    19501 ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc cgctaacaga
    19561 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    19621 ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    19681 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    19741 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    19801 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    19861 ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc tgaataccga
    19921 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    19981 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg acgacggcgt
    20041 caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    20101 ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca acaagaggaa
    20161 gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    20221 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    20281 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    20341 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    20401 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    20461 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    20521 gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga gaatatggtg
    20581 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    20641 agctacggat
//
LOCUS       DAJTNW010000075        20570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNW010000075 DAJTNW010000000
VERSION     DAJTNW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            409..1239
                     /locus_tag="OO179_002859"
     CDS             409..1239
                     /locus_tag="OO179_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5532852.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO179_002860"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO179_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5532853.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO179_002861"
     CDS             complement(2368..3828)
                     /locus_tag="OO179_002861"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5532854.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO179_002862"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO179_002862"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5532855.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO179_002863"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO179_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5532856.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO179_002864"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO179_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5532857.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO179_002865"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO179_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5532858.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO179_002866"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO179_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5532859.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO179_002867"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO179_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5532860.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO179_002868"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO179_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="OO179_002869"
     CDS             complement(12470..13009)
                     /locus_tag="OO179_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5532861.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO179_002870"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO179_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5532862.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OO179_002871"
     CDS             complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OO179_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5532863.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OO179_002872"
                     /pseudo
     CDS             complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OO179_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17232..17948
                     /gene="nanC"
                     /locus_tag="OO179_002873"
     CDS             17232..17948
                     /gene="nanC"
                     /locus_tag="OO179_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5532864.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17968..19074
                     /gene="nanM"
                     /locus_tag="OO179_002874"
     CDS             17968..19074
                     /gene="nanM"
                     /locus_tag="OO179_002874"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5532865.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19139..20119
                     /locus_tag="OO179_002875"
     CDS             19139..20119
                     /locus_tag="OO179_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5532866.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4673 g         5511 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
    14041 cagtttggcc ccaattgtct tgtgtttatt tgttttttaa atttattatc aataagttaa
    14101 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg
    14161 caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt cgattcccga
    14221 gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa ccgcaagcat
    14281 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
    14341 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
    14401 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
    14461 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
    14521 ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca ctaatacgca
    14581 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
    14641 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
    14701 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
    14761 aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct atcagtcaat
    14821 tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta ttttcattga
    14881 tttgattcaa tataacaata gattacagag gattaacttc cagttactta aaaaataaag
    14941 aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg attttgttta
    15001 tttattttga agaacataaa ccattagatt ttgtatgggt atggttaact taatgtttat
    15061 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
    15121 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
    15181 aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
    15241 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctagataa
    15301 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatagcgt aacatgtgcg
    15361 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
    15421 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
    15481 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
    15541 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gctttaagat
    15601 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
    15661 gcaaaatcag acaataatta cgtgttgcat gaggcccggt atttgctgct ttaaggagtg
    15721 actcgatttc actatgggta aggaagttcc tttttttgtt atcagcctta ttcctcatcg
    15781 ttttccctta taattacaga cgcgcactag ctgtgctggg ttagcaataa tccaacattt
    15841 ttatcctgat tatgtttata aaagcgaacg tttgcttaat aacttacgta agtggaccag
    15901 ttcttctgag cgaacttaaa tggagtagcg atagttactt ataataactt tgccatacaa
    15961 acgccatgct cttgcatgct atgtaccttt atatatttat caatctgata cgaatctaat
    16021 aataaccctc atcattaatg caactaatct tatctatata tcatgtgata tgttctgtaa
    16081 caagtaaaat caacataaaa aattcagcaa aatttaatat ttgttaaact agccgtctca
    16141 aatatgacat tattagttaa ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta
    16201 atttcttagt ggctattatc atgctaattc ttagatccct agcgattatt cctgcctggt
    16261 tcgtcagctt aggccatgag attgttacat tcctttttat cctggagata tgggggaacg
    16321 caggcgacgc tttttcatct acatccgttg gtaaatatag ctttggtcac tacgacttaa
    16381 gccttcctca atcacagcta ttcgccgggc gatacggcaa acaaactaag tgaaacatca
    16441 tcctgaaaac accaacatca acaagcctct ccagatcgac atcaggagtg accagttaca
    16501 agccacaaac caagaaacat atttcatgat gagaattatg ctcagcctga tggcgagtaa
    16561 acccttattt tcaccactct cactgagtta gcttgcaaag ctttctttgt ttagattcta
    16621 aataaattat ttataaaaca tatagttaaa agaaaatctt tagtttttga agaccaccgc
    16681 aagtgttcgt ctggcttcac atggcatctt cttcttagaa aaagatcgac atattttgtg
    16741 acgcgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
    16801 ggtataaaga tgcaccacga tagtcaatga gactgggcat ctcgtaatct atggcaaaca
    16861 tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa
    16921 aatgagatca acagacactc tattttatga ctctgggtaa aatgggttga gtaagtgata
    16981 taacttacga atattcaaat caattaaaaa tcagaagaga ttttatactc aggtatttaa
    17041 tctggatctc tgtttattta aataatgtta aaagagaatt ttcacaggag accttataca
    17101 aaaaatataa aatacagtta cccgttgcca aagacactat aagcctggca aaaaaatatt
    17161 acacaacata aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat
    17221 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
    17281 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
    17341 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
    17401 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
    17461 atgttcaggt tgaagttaat tacgcaatta aacttgatga tcaatggacg gtgcgcccgg
    17521 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
    17581 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
    17641 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
    17701 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
    17761 agaatgatta tcgctatgca aaccacaaga aatgggcgac ggaaaatgcc tttgttctac
    17821 aataccatat gacgcccgat attacgccat acatagaata tgactacctt gaccgccagg
    17881 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
    17941 aactgtagta gacaggagac agtcacaatg aataaaacta taatggcgct tgctatcatg
    18001 atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt
    18061 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacagca
    18121 tggtacaagc tagatacaca ggccaaagat aaaaaatgga ctgcgttagc tgcattccct
    18181 ggtggaccga gagagcaagc aacctctgca tttattgatg gcaatctgta tgtgtttggc
    18241 ggcattggca aaaacagcga gggattgact caggtattta atgacgtaca caaatacaac
    18301 cccaaaacca atagctgggt taaattgatg tcgcacgcgc cgatgggcat ggcgggtcat
    18361 gtgacttttg tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc
    18421 aatggctatt ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc
    18481 aacgcccact attttgacaa aaaagcagaa gattatttct tcaataagtt tctgttgtct
    18541 tttgatccct caacacagca atggagttac gctggcgaat cgccctggta cggaacggct
    18601 ggtgcggcgg ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca
    18661 ggattgcgaa cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat
    18721 aagcttgatc ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat
    18781 gattctctta tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac
    18841 ggtaagaact atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg
    18901 cataacggga aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca
    18961 ttgccctgga ataatagttt attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg
    19021 acggattcag ttttaatttc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc
    19081 agggccccgg taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat
    19141 gaacgcaata atatcacccg attattacta tgttcttacc gttgctggtc agtctaatgc
    19201 catggcgtat ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat
    19261 taaacaatta gcgagatttg cgcatacgca tcccggaggc ccgacatgtc actttaacga
    19321 cattattcca ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc
    19381 tctggcaacg aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg
    19441 gaaattactg cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg
    19501 cggatcggct tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca
    19561 tgatgcttgt cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg
    19621 agccgcactg gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga
    19681 atttgactta atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga
    19741 agcctttcgt agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc
    19801 gtggttttgc ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc
    19861 tatttatggc aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca
    19921 acaaggtgaa agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg
    19981 atattacggt tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca
    20041 tttcagcacg gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca
    20101 gttttggcgc gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca
    20161 gagtagacaa caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac
    20221 agcaggcaat tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac
    20281 cgttgttatt gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta
    20341 tggctcggaa atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc
    20401 gcagcaccgt cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga
    20461 cgatgatgat atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc
    20521 attttctttc attcctaaaa gcacccgcca ttaatcccag gcgcattatg
//
LOCUS       DAJTNW010000076        20521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNW010000076 DAJTNW010000000
VERSION     DAJTNW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO179_002876"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO179_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5532867.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO179_002877"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO179_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5532868.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO179_002878"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO179_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5532869.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO179_002879"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO179_002879"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5532870.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO179_002880"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO179_002880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5532871.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO179_002881"
     CDS             4449..4814
                     /locus_tag="OO179_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5532872.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO179_002882"
     CDS             4964..5482
                     /locus_tag="OO179_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5532873.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO179_002883"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO179_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO179_002884"
     CDS             6713..7195
                     /locus_tag="OO179_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5532874.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO179_002885"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO179_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5532875.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO179_002886"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO179_002886"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5532876.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO179_002887"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO179_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5532877.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO179_002888"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO179_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5532878.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO179_002889"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO179_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5532879.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO179_002890"
     CDS             10939..11730
                     /locus_tag="OO179_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5532880.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO179_002891"
     CDS             11751..13037
                     /locus_tag="OO179_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5532881.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO179_002892"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO179_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5532882.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO179_002893"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO179_002893"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5532883.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO179_002894"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO179_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5532884.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO179_002895"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO179_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5532885.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO179_002896"
     CDS             complement(16985..17275)
                     /locus_tag="OO179_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5532886.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO179_002897"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO179_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5532887.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO179_002898"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO179_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5532888.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO179_002899"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO179_002899"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO179_002900"
     CDS             19137..20378
                     /locus_tag="OO179_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5532889.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5303 a         5357 c         4814 g         5047 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gactggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJTNW010000077        20490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNW010000077 DAJTNW010000000
VERSION     DAJTNW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            113..1168
                     /gene="phoE"
                     /locus_tag="OO179_002901"
     CDS             113..1168
                     /gene="phoE"
                     /locus_tag="OO179_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5532890.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OO179_002902"
     CDS             complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OO179_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5532891.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OO179_002903"
     CDS             complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OO179_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5532892.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OO179_002904"
     CDS             complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OO179_002904"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5532893.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3722..5179
                     /gene="pepD"
                     /locus_tag="OO179_002905"
     CDS             3722..5179
                     /gene="pepD"
                     /locus_tag="OO179_002905"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5532894.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5236..5850)
                     /locus_tag="OO179_002906"
                     /pseudo
     CDS             complement(5236..5850)
                     /locus_tag="OO179_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5912..6955)
                     /locus_tag="OO179_002907"
     CDS             complement(5912..6955)
                     /locus_tag="OO179_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5532895.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7201..7653)
                     /locus_tag="OO179_002908"
     CDS             complement(7201..7653)
                     /locus_tag="OO179_002908"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5532896.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OO179_002909"
     CDS             complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OO179_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5532897.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OO179_002910"
     CDS             complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OO179_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5532898.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OO179_002911"
     CDS             complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OO179_002911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5532899.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OO179_002912"
     CDS             complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OO179_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5532900.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10292..12004
                     /locus_tag="OO179_002913"
                     /pseudo
     CDS             10292..12004
                     /locus_tag="OO179_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OO179_002914"
     CDS             complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OO179_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5532901.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12894..13415)
                     /locus_tag="OO179_002915"
                     /pseudo
     CDS             complement(12894..13415)
                     /locus_tag="OO179_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13799..14539
                     /locus_tag="OO179_002916"
     CDS             13799..14539
                     /locus_tag="OO179_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5532902.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14510..15277)
                     /locus_tag="OO179_002917"
     CDS             complement(14510..15277)
                     /locus_tag="OO179_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5532903.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OO179_002918"
     CDS             complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OO179_002918"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5532904.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16301..18745
                     /gene="fadE"
                     /locus_tag="OO179_002919"
                     /pseudo
     CDS             16301..18745
                     /gene="fadE"
                     /locus_tag="OO179_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OO179_002920"
     CDS             complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OO179_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5532905.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19415..20185
                     /gene="yafV"
                     /locus_tag="OO179_002921"
     CDS             19415..20185
                     /gene="yafV"
                     /locus_tag="OO179_002921"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5532906.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20295..20490
                     /locus_tag="OO179_002922"
                     /pseudo
     CDS             20295..20490
                     /locus_tag="OO179_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5010 a         5264 c         5270 g         4946 t
ORIGIN      
        1 gaatatcatt aatctgtaat atatctttaa caatctcagg ttaaaaactt tcctgttttc
       61 aacggggctc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga aaatgaaaaa
      121 gagcactctg gcattagtgg tgatgggcat tgtggcatct gcatctgtac aggctgcaga
      181 aatatataat aaagacggta ataaactgga tgtctatggc aaagttaaag ccatgcatta
      241 tatgagtgat aacgccagta aagatggcga ccagagttat atccgttttg gttttaaagg
      301 tgaaactcaa attaacgatc aactgactgg ttatggtcgg tgggaagcgg agtttgccgg
      361 aaataaagcg gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg ccgggttgaa
      421 atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt atgacgtgga
      481 agcctggacc gatatgttcc cggaatttgg tggcgattcc tcggcgcaga ccgacaactt
      541 tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct tcggcgttat
      601 cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg acgttaaaaa
      661 gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca gcgatttcgc
      721 cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc aaagccgtgg
      781 cacaggcaag cgtgcagaag catgggcaac aggtctgaaa tacgatgcca ataatattta
      841 tctggcaact ttctattctg aaacacgcaa aatgacgcca ataactggcg gctttgccaa
      901 taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc tgcgtccatc
      961 gctgggttat gtcttatcga aagggaaaga tattgagggc atcggtgatg aagatctggt
     1021 caattatatc gacgtcggtg ctacgtatta tttcaacaaa aatatgtcag cgtttgttga
     1081 ttataaaatc aaccaactgg atagcgataa taagctgaat attaataatg atgatactgt
     1141 cgcggttggg atgacgtatc agttttaatg aatattgccg gatgtgatgc atccggcaca
     1201 tttcactcac gccgttaact tcaccggttc atcacgaaaa tcattcgccg gttccagctt
     1261 cagattcagc gtcgtcagca gttcacgcag cttctcgtga aactcacgca gggtgtgctc
     1321 cagtcgttca accacttcag tatcttttac cggaacactc ttccagtcgc ctgctttatc
     1381 gaacagacca aactggtagc tgtaggtaaa acgggattcc tgcgcttcca gctccatcca
     1441 ccagccccag aattcacgca cttccggtgc cggtttcacg ttgacgcata cagccagaca
     1501 atcgaaaaag aatcgattat cttcgcactt accttcacga atatacgggc ctagtgcggt
     1561 aaattttttg atcaatctgc tcttcgggtg tccactcggt aacgtcattg ctatctcctg
     1621 ttgtgatgca actgttttac caaattggca aaatttagca aatttttaac acaagcgttt
     1681 ttcgatccag ccggtgattt cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa
     1741 tgggatctct aataatttac cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc
     1801 cgggctgaac ggatcgttct tccagtagcc tgataacatt ggcgttgggc agcgacgccc
     1861 cagcaatcct tgcactttta atgaatagcg attcagctcc acgcgtaacg cttcgtcgga
     1921 agcatcatgc atccccaaac gactcgccag aacgtcaaga tacatttccg gcacctgttg
     1981 ctggcactta aaatcactca acagggtatg aactaccgga ccaagacagg caaccgcttt
     2041 cagacgcggc gattcaaggt atgccagacg cacggcaacg ttagcgccga aacggaaacc
     2101 aaaggccgcg acgcgagtgt gatccaccca cggtacgtta ggcagcgcct ttaagacgtg
     2161 ctgatgcaac aggctggagt cctgggtgag cttccatttt gaagaaaagc ccaccgacgg
     2221 catatcaata gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat acaggctgta
     2281 atagtccgtc tgcatcgcat ccagaccacc acacattaat actgtcggga acgggccatc
     2341 gcctttcggc atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg taaactccat
     2401 ctgccgcatc gtgcccggca gacgctgggc ggcctcttca taggcgcggt ttgataaagc
     2461 ctgcgcttgc tcggccaggt catctccttt cagatgagga taggcggcaa tgttgtacaa
     2521 cgtagccgca tgcagccagt gtcgaccact gagtttcgga tcttcttccg tacaggcttt
     2581 ttgttgccac accatcgcct gggtggccca ctcataaatc cagttgccac cacggtagcc
     2641 aatcacggta tcgtataaat catcgtcggt acgttcagca tcgctcatca caatacgtgc
     2701 ctggacgtcg aggatttcac gtgggtcaat accgcgccag atccacatca gacggttaat
     2761 catgcgatac cagtgaggga tggttttacc atcaagagct gactgcacag gcggttgtgc
     2821 gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa
     2881 cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa
     2941 ctattgtaac ctgcctgaag ttaaaaagaa caacgcccgg caatgccagg cgttgaaaag
     3001 attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc tgttcgatcc
     3061 aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg
     3121 cgaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaaca gcagtaccac
     3181 cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca
     3241 gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat
     3301 gacgaatacc cagttcacgc gccagtaacg cccccggtac cagaccgcca cggcttacgg
     3361 caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga
     3421 tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt
     3481 tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg
     3541 gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg
     3601 acgcgggata aagtggtatt ctcaatacat atctcgcaag cctgtcttgt gttgacaaca
     3661 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
     3721 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
     3781 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
     3841 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
     3901 tgctaccgca ggtatggaaa atcgtaaacc ggtcgtctta caggcccacc tcgatatggt
     3961 gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc agccttatat
     4021 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
     4081 ggcctctgca ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
     4141 gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga gcaactggtt
     4201 gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct acatgggttg
     4261 tgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
     4321 ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg gcggtgaaat
     4381 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
     4441 agaactggac ctgcgcctta tcgatttcaa cggcggcaca ctgcgtaacg ccatcccgcg
     4501 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgcactga aatctctggt
     4561 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcctt
     4621 gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
     4681 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aattccgacg tggcaaaagg
     4741 tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
     4801 tcactgcctg atccgttcac tgatcgacag cggtaaagac tacgtggtga gcatgctgga
     4861 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
     4921 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc gcctgttcaa
     4981 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
     5041 gtatccggaa atggacatgg tttctatcgg gccaactatc accggtccac actctccgga
     5101 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa
     5161 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
     5221 cagtttcatc cttcattatc cttcgataaa agccatccct gtaaacgttc gtcgcgggtt
     5281 gccacgttca atctggtgat ggaacattcg ccgctgtgat ttcagcaccg cgctattttc
     5341 ctattgctgc tgatccagct tccaggcaat cagcaatcgc gccagccgtt tgttagcatg
     5401 ctggctgcgc tctgactgaa ccttcacgct aataccggat gccaaatgcg tggcgcgtac
     5461 cgccgagtcg gttttattga catgttgacc gcccggcccc gacgaacgca gcgtctcata
     5521 ccggatcgca tcggattgct cctgctcatc agcggtaaaa cgcccaatgc ccagaaacca
     5581 gtttttacgc ccgtgatgtg ggcgatacgg gctcgggcaa atccactgaa tagtgccaca
     5641 ccagctttcg cttaacgccc atgcgttgtc gccatccaga gaaatcagcg ccgaacgcag
     5701 cgtgtcagag tagcggcctg tttctgtttc cagcaccgtt accgcgacgt cttgtcgggc
     5761 agcttcttta attagcctgt ccagcgcctt tttcactgcg agacaacatt cttccggccc
     5821 ctgcgctgag gagagttata gcaagatcat ccttttttcc ctccactgtt tttgagcgtt
     5881 agcaccggac gcagccgcgc gacaggaata attaatccgg cctgcccaga cattgcacca
     5941 cactttcagc cgatttatag gcttgtggcg cttcttcgaa gatgagttgt ttatcgcgac
     6001 aaattacccg actgccaagt tcagtacgcg agagttgcgt cgctgtgtat ttcgctgcca
     6061 gacgcccttt acactcggtg cgcccccatt tacgcccagc cccatgcgcc agcgaatgca
     6121 atgtttcctc actcctgacg ggctgaacca gcctggagta atcaccgcgt gaaccgggaa
     6181 taaccaccag accacagtca tcaggcgtgg cacctttacg atgtaagacc ccttgttgat
     6241 caccgattcg acacgcgcta acaaagttat gcgccacatc cagaaccgga cttccggtgg
     6301 ccttaacctg ttgcattatg cgcaaagcga tcaggtggcg attaaaacgc gcaaacgcca
     6361 gcgcgtcgtc actgccttca ggcaaaccat gatgcgaaaa cgaggcaata tgccgctgta
     6421 aaatagactg ccctagcccc ctcgagccgc tatgaaccag cagttgcaga tgctgcgcat
     6481 ccagacctgc cagtgcaaac agttcagcgt tgatgatttg atcaacttgt tgcagttcag
     6541 cgaaatgatt gccaccaccg atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg
     6601 ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaaa gctgataatc
     6661 gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc catagcgcca
     6721 tgccacagcc gatatcattg ccgaccagtg ctgggtaaaa acggcctgcg gagaagaacg
     6781 ctgcgccaat cgggtagccg cgtccggggt gtaaatctgg catccccact acgcgctgca
     6841 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
     6901 catcagatgc gatggtaaat accgcatcgg ataagggacg aatataattg cccatatacc
     6961 attccgtaaa tagactcgtc atacttcaag ttgcatgtgc tgcgacttga attattgaga
     7021 gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa aagaattaat
     7081 cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac ctccttgcag
     7141 tttgtggaat aagaaaacgc gcacatacta ggcgggcgtt attttcattg caagctggat
     7201 ttaatgttgc ggtttataac gcatataaaa attaataaac cacgctcccc ggcattcaac
     7261 gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg cggttatgct
     7321 tgcgtccacc gtaagttcgg attcagactt aataaaaggt tttaacaaag cgctggcaac
     7381 cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atatagtgtt caacgcaggt
     7441 aataaagccg accagttttg cattaataac tgcaacccgc acttgtgatt tcgcgagttt
     7501 ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgta gtgaataatg
     7561 ctgactgacg gtcatcgtaa ccgctctaat gaaaatagcg catagttgct gaaaatcgcc
     7621 aggatgataa tctcttattt gtatgttatt cataatataa attcaaaaac gcatgcgaaa
     7681 cgcttctgcc atttttccaa ttttatgcat ttggttgtta tctcgagcca gtttatgagg
     7741 ttcaggcttc agaatggcaa tgagcaacca tgcttgctca tcaaacgcac cctgacaata
     7801 taccaaatgc gcttcgtcat tcgttctgct gaattggcgc aactgtggcg gaaatggatt
     7861 attcacattt gccagatgaa tatgagcaac tcgctcaaat ttgattaatg gccaggtaaa
     7921 cgagtcgtcg tagagtgcat cgcgaccaaa tatatctggc aaaacaccgt cacgcttata
     7981 ggaaataaaa tccgccgtta acgcatcaag ttcctctgct gtaagttgca ggcgaataag
     8041 ttttgttttg aatacccgca tccttattcc ttaaagtcat tgaaatcatc atccgtcata
     8101 tcattaagat attgtttagc gcggcgtgta atttcctcta atcttgcagc acttggacga
     8161 aagcgcgcct ttatgggctt tttctcctct tgttcagcaa gcatgtccat taacgcttcg
     8221 aatgcgctgg cacttaagag atatcctgcg gggcgattat tagaaagaac cgcaactggt
     8281 tgatcaataa agtatttagc tgggttttta cgtaactcag tgatattgac cgatttttca
     8341 gcgagaattc gatgcataca gtgataccct cataataatg cacaccagaa tatacataat
     8401 agtatacatc ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca
     8461 tgcagcccca ccagacgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
     8521 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgggt ggtctgctga
     8581 aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg ttttaccttt
     8641 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
     8701 tgatgaatat cttccgccat cgtgcgttcc acgccgacgg attttcgtaa ccgttcgctg
     8761 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
     8821 aagcgtttaa gcagaatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc
     8881 atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggattttttc cagtggtaag
     8941 gtttgtaaaa atgccggaac ttctgccggc gtaatcacaa actgaccgtt gggtttattc
     9001 atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga tgccgtcagt
     9061 tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
     9121 caatggacgc tatcggtgac atcgagataa gcctcatcca gtgacaacgg ttcaatgcgc
     9181 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
     9241 cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatcccagt aggcatagcg
     9301 ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
     9361 cggctgccgc caatagcaat agggatatcg cgcagagcgg gattgtcgcg catctccacc
     9421 gctgcgaaaa agcagtccat atccacatga atgattttac gcattgctca cctctcaaca
     9481 ctggtaaagt atacagtgat ttcaggtttt gagaaatgcg taaagattca gcatttagcg
     9541 cattcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
     9601 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
     9661 aatctcaata cgccgattgc ccgcgctttg cggattcttc gcgtccagca gcatctggtc
     9721 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
     9781 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
     9841 ggccatcgca tcggtatgcc cggtaataat aattttgtta tagagcgagt cgaacactgg
     9901 cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
     9961 catattgcga ttctggtcgt ctttaatcag cacgcgtaat ccctggggaa caatctccat
    10021 ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccaactc
    10081 gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc
    10141 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
    10201 gattttattt aacggcgaca gcccaccacc gttaaaaatc gactgcccgt gcagcgcggc
    10261 gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta tcgtcgtcac
    10321 caaagaggcc gagcgtattt ccgaggtttc tgcccgcttt accctggacg cgatgcccgg
    10381 caaacagatg gcgattgacg ccgatcttaa cgccggattg atcaaccagg cgcaggcgca
    10441 aacccggcgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
    10501 gaagtttgta cgcggagacg ccatcgccgg gatgatgatt ctggcgatca acctgagcgg
    10561 cggcgtctgt atcggcatct tcaaatacaa cctgagcgcc gacgccgcct tccagcaata
    10621 cgtgctgatg accatcggcg acggcctggt ggcgcagatc ccttccctgc tgctctccac
    10681 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
    10741 ccaccagttg ctggcaagcc cgtcggtgct ctacaccgcc accgggatca tgttcgtgct
    10801 ggcggtggtg ccggggatgc cgcatctgcc gtttttgctg ttcagcgccc tgcttggctt
    10861 taccggctgg cggatgagca aacgcccgca ggcggcggag gcggaagaga aaagcctcga
    10921 aacgctgacc cgcaccatca ctgaaaccag cgaacaacag gtcagttggg aaactattcc
    10981 gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg tggacaaagc
    11041 gcagggcaac ccgctcaccc agcggattcg cggcgttcgg caggtgattt ccgacggtaa
    11101 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgcctcaagc ccagccagta
    11161 cgctattttc atcaacggca ttgaggctga tgaagcggat attccggcgg ataaactgat
    11221 ggcgctgccc tccagcgaaa cctacggcga gattgacggc gtgctgggga acgacccggc
    11281 gtacgggata ccggtcacct ggatccagcc tgcgcagaaa gcgaaggctc tgaatatggg
    11341 gtatcaggtg atcgacagcg ccagtgtgat tgccacccat gtgaacaaga ttgtgcgcag
    11401 ctatattcct gatttgttta actatgatga catcacgcag ttgcataacc gtttgtcgtc
    11461 gatggcaccg cgcctggcgg aggatttaag cgcggcgctc aattacagcc aattgctgaa
    11521 agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
    11581 cgtgctggtc gccagtagca cggtgactaa agatcatatt ctgctggcgg ccgatgtgcg
    11641 cctggcgctg cggcgcagca ttactcatcc gttcgttcgc aagcaggagc tgacggtgta
    11701 tacgctgaat aatgagctgg aaaatctgct gaccaacgta gtgaatcagg cgcaacaggc
    11761 cggcaaagtg atgctcgaca gcgtgccggt tgacccgaat atgctcaacc agttccagag
    11821 cacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagatccgg tgctgctggt
    11881 gccgccgcag ctacgccctt tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca
    11941 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg gggcgttgat
    12001 gtaagaatcc ggcatcggtg cacaattgcc ggatgcggcg taaacgcctt atccggccta
    12061 ccgttcaggc acaagttttt aggcatgata agacgcgcca gcgtcgcatc aggcatctgc
    12121 gcataactgc cggatgcggc gtgaacgcct tatccggcct accgttaaag cacgatttgt
    12181 aggccggata agcgtaagcg catccggcac cagcgcacag ttataaaatg atacgctccc
    12241 cggcacttaa atccgttatg tctcccgccc aatcgatggg atataaccct cgcgcgacat
    12301 cgcgatggaa cgttgagaat ggccaatcac tcacctgctt tacccagcca tgtttctctg
    12361 gatttatata aatataatca acatgatggc gataatcttt tgtattgcga atggcatgct
    12421 cccaaaaacg cggttgccag atatttttca atccgcaagc atgggtgaac tgctttttaa
    12481 tctcccgcca gcgagaggaa aaatcatcat cgctttcggg taatgtccat atgcaatgca
    12541 tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc ttaacgttaa
    12601 taatggcgtt acggagcgtc tggaactggg tggtcagaag atggcttcga cgattgcgta
    12661 aatttaccgt gaaaaaccat gttcccccct tgatgtaata acgacgatat tccgacatag
    12721 cgactccttt ttctcaacgg taagaggaaa aggaaaggcc gacaatgacg ctgtgttata
    12781 tcgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg cgtcagcgtc
    12841 gcatcaggca tccgtgcaca accgccggat gcggcgtaaa cgccttatcc ggcctacaaa
    12901 atcatctctt ccgtcaaaat cctgcgcgcg cctaaaaaat ggtcctgcca gaaaggttcg
    12961 gctaaccggc ttacccgaat ggtttccccg gtgcgcagtg actcaataaa ctgaccgtcg
    13021 cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa aaatagcaaa
    13081 tctccccggc gcaggtcgtt attcgccaca atcgttgccc gacggtagtg gtacatctca
    13141 ttggccgtgc gcgggagttt cgcctcaagg atcttgttgt aggcataaaa aactaaccca
    13201 ctacaatcga agccttgatc gggccgcgtg ccgccccaga cgtaaggctt gccaagctgc
    13261 tgctccaggc ggtgaatcgc cacttcggtg atgttatgca ggtgatcgct gcttaaaaag
    13321 tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg cgcaggaaac
    13381 cattctggat gctgttttat ctgctcccgg ttgtggtggc gctggcgagt aaagggagca
    13441 aaacgcataa cgaaaacacg cctggatgca aaaatctgcc gagcaagaac gaagacatga
    13501 aagacataaa ccggctgaat gaaggtgaga attttaaaag accggaggag taaacgggat
    13561 tattaattgc ttatttgcat attactattg agatttcata atattaggaa ataaatgata
    13621 catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac ccagcgccga
    13681 caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga
    13741 tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat
    13801 gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt
    13861 gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg
    13921 atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatcttt acgtcaaaat
    13981 gggcgagcaa tatcaactgc tcgacagtta taaaatctgt aaatattccg gcggcttagg
    14041 gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa
    14101 tcagttaaaa ccagacagcc gttactacaa agcgattaat attggattcc ccaatgctta
    14161 tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt gtgtatccat
    14221 cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc
    14281 gctggtgttt ggtcagccga gcgtgcaggt gagtatttac ccattccgca tgaccgacgc
    14341 caatatgaag cgccataaat attccaactt taaggacttc tgggagcagc tgaagccggg
    14401 ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggcca atggccgtta
    14461 cgtggtcagc aagccgttaa gccacgaagt ggtgcagcca caactggcat caaactacac
    14521 gctccccgag gcaaaataag tgccattcgc ctggcataat tttttgccag gtttcattgc
    14581 ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga
    14641 agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga
    14701 tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtccgaa agcagcatgt
    14761 tgaaaacgcc cttcgcccgc agttcatccg ccagtgaggc gatatattta aacactgccg
    14821 ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc cagcaaaagg
    14881 ctttttcgct gtcggtttca ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc
    14941 ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg cgggtaaatg
    15001 ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg
    15061 aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt
    15121 gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt
    15181 tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg
    15241 tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac aacagcggga
    15301 gcgcatggac cccggcgaag attgatttaa gaattttcag gtcggatgcg gcgtaaacga
    15361 attattcggc ctacggttcg ggtacagatt tgtaggcatg ataagacgcg tcagcatcgc
    15421 atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg cctacgccag
    15481 acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga
    15541 atctcctgaa tgcggtcggc ataaccaaag tgcggaacgc gaatttcgat atccgccgtg
    15601 ccagccattt taccgccgtc tttaccggtc agggtgatca ctttcatccc cttctcacgc
    15661 gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat ccccagcagt
    15721 acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt gaaaccgaaa
    15781 tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga
    15841 cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa
    15901 ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc
    15961 gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc
    16021 gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata
    16081 aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt
    16141 gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg
    16201 ttgtaattat attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc
    16261 acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct
    16321 cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag
    16381 tctgattttg ctcgcctaga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt
    16441 actggtgcct ctgaccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat
    16501 gatttccgcg ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga
    16561 gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc agggcaagcc
    16621 ggactggaaa aagctgcata actatccgca gccgcgcctg accgctgaag agcaagcgtt
    16681 tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga ttactcatga
    16741 gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct tcttcgcgat
    16801 gattatcaaa aaagagtacg gcggactgga gttctcagct tatgcccagt ctcgtgtgct
    16861 gcaaaaactc tctggcgtga gcgggatcct ggcgattacc gtcggcgtgc caaactcatt
    16921 aggcccgggc gaactgttgc aacattacgg cactgacgag cagaaagatc actatctgcc
    16981 gcgtctggcg cgtggtcagg agatcccctg ctttgcactg accagcccgg aagcgggttc
    17041 cgatgcgggc gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca
    17101 ggtgctgggg atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac
    17161 cgtgcttggg ctggcgttta aactctccga cccggaaaaa ttactcggcg gtgcagaaga
    17221 tttaggcatt acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg
    17281 ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag atgtcttcgt
    17341 gccgatcgat tacatcatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt
    17401 ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa
    17461 atcggtagcg ctggcaacgg gcgcgtattc tcacattcgc cgtcagttca aaatctctat
    17521 cggtaagatg gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat
    17581 ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct
    17641 gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat
    17701 ggatattacc ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta
    17761 ccagggcgca ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat
    17821 gatcttcgga caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc
    17881 gaagaacaat gacgtcaacg cgttcgataa actgttgttc aaacatatcg gtcacgtcgg
    17941 tagcaacaaa gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc
    18001 aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc
    18061 cctgctttct gatgtctcga tggcactgct gggcggcagc ctgaaacgtc gtgagcgtat
    18121 ctcggcacgt ctgggggata ttttaagcca gctttacctc gcctctgccg ttctgaagcg
    18181 ttatgacgac gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga
    18241 tgcgctgtat caggcagaac aggcaatgga caatttactg caaaacttcc cgaaccgcgt
    18301 ggttgccggg ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc
    18361 tgacaagctg gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgttcacg
    18421 tattggtcgc ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga
    18481 ggcgttggtg gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctggg
    18541 taaaaatctg ccatttaccc gtctggatga actggcgcac aacgcgctga cgaaggggct
    18601 gattgataaa gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat
    18661 taacgttgat gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa
    18721 agtgcggaaa gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat
    18781 ccgctaatta tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg
    18841 cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt
    18901 tctcttgcga cgttttctca tcaatagccg agaacagccc cgtcatctga ttagatttct
    18961 cggaccacat cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca
    19021 cctgatacgt ctcatctccc aacgttacgg tttgtgcggg agtataagta ccgcctttca
    19081 tcacccatgc aggtagctta tgcccttgca ccatctgatt aaacgcagct ttggtggttt
    19141 cgccctttgc aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga
    19201 ccagtgcggc gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca
    19261 tgttattaac ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa
    19321 tatcagcatt tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag
    19381 ttataaaacc cttcgcataa aggagttgta caccgtgcct agtttgaaga ttacgctttt
    19441 gcagcaaccg ttggtgtggg tggatggtcc tgccaacctg cgccattttg atcgtcaact
    19501 ggaaggtatt accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc
    19561 catggaagcg gcagcttcgt cgcttgcaca agatgacgta gtgaactgga tgacagctaa
    19621 ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc
    19681 ggttaaccgc tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca
    19741 tctgttccgc atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt
    19801 ggaatggcgc ggctggcgta ttttgccgct ggtgtgctac gacttacgtt ttccagtgtg
    19861 gtcgcgcaat ctcaacgatt atgacctcgc cctgtacgtc gccaactggc cagctccgcg
    19921 ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc
    19981 gggatgcaat cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt
    20041 gattaatccg caaggcgaga ttatcgctac cgccgacgcg cataaggcaa cgcgtattga
    20101 tgcggagctg tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga
    20161 tgcggatgag tttaggttgt ggtgagtggg ggttgtgttt agggccgggg gctgggctag
    20221 attacgaaag cccctaactt aaacacaata ttacgccaga agtcaccatt tccaaaacac
    20281 agcgtcgctg taccgtgggt tatctgccca accgcagcga cacgccggtg atcaaaatca
    20341 gcgccaaatg gctgagaaaa gcgggatttg agatcggcac gggcgttacc gtgaagatct
    20401 cggagggatg tttgatcctg cttgggataa caacgaagta caggaactgc gcagggaact
    20461 ttatcaggtt aaacagccag ttaaggggat
//
LOCUS       DAJTNW010000078        20275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNW010000078 DAJTNW010000000
VERSION     DAJTNW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(59..817)
                     /gene="aroD"
                     /locus_tag="OO179_002923"
     CDS             complement(59..817)
                     /gene="aroD"
                     /locus_tag="OO179_002923"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5532907.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIAHVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(848..1714)
                     /gene="ydiB"
                     /locus_tag="OO179_002924"
     CDS             complement(848..1714)
                     /gene="ydiB"
                     /locus_tag="OO179_002924"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5532908.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1726..2997)
                     /locus_tag="OO179_002925"
     CDS             complement(1726..2997)
                     /locus_tag="OO179_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532909.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3180..4385)
                     /locus_tag="OO179_002926"
     CDS             complement(3180..4385)
                     /locus_tag="OO179_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5532910.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4485..4841)
                     /locus_tag="OO179_002927"
     CDS             complement(4485..4841)
                     /locus_tag="OO179_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5532911.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4977..5084)
                     /gene="rprA"
                     /locus_tag="OO179_002928"
     ncRNA           complement(4977..5084)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO179_002928"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5270..6382)
                     /gene="ydiK"
                     /locus_tag="OO179_002929"
     CDS             complement(5270..6382)
                     /gene="ydiK"
                     /locus_tag="OO179_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5532912.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6771..9827
                     /locus_tag="OO179_002930"
     CDS             6771..9827
                     /locus_tag="OO179_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5532913.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9824..10234
                     /gene="menI"
                     /locus_tag="OO179_002931"
     CDS             9824..10234
                     /gene="menI"
                     /locus_tag="OO179_002931"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5532914.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10334..10522
                     /locus_tag="OO179_002932"
     CDS             10334..10522
                     /locus_tag="OO179_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5532915.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11070..11438
                     /gene="sufA"
                     /locus_tag="OO179_002933"
     CDS             11070..11438
                     /gene="sufA"
                     /locus_tag="OO179_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5532916.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11447..12934
                     /gene="sufB"
                     /locus_tag="OO179_002934"
     CDS             11447..12934
                     /gene="sufB"
                     /locus_tag="OO179_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5532917.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12944..13690
                     /gene="sufC"
                     /locus_tag="OO179_002935"
     CDS             12944..13690
                     /gene="sufC"
                     /locus_tag="OO179_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5532918.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13665..14936
                     /gene="sufD"
                     /locus_tag="OO179_002936"
     CDS             13665..14936
                     /gene="sufD"
                     /locus_tag="OO179_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5532919.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14933..16153
                     /gene="sufS"
                     /locus_tag="OO179_002937"
     CDS             14933..16153
                     /gene="sufS"
                     /locus_tag="OO179_002937"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5532920.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16166..16582
                     /gene="sufE"
                     /locus_tag="OO179_002938"
     CDS             16166..16582
                     /gene="sufE"
                     /locus_tag="OO179_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5532921.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16731..17735
                     /gene="ldtE"
                     /locus_tag="OO179_002939"
     CDS             16731..17735
                     /gene="ldtE"
                     /locus_tag="OO179_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5532922.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17798..18034)
                     /gene="lpp"
                     /locus_tag="OO179_002940"
     CDS             complement(17798..18034)
                     /gene="lpp"
                     /locus_tag="OO179_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5532923.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18345..19757)
                     /gene="pykF"
                     /locus_tag="OO179_002941"
     CDS             complement(18345..19757)
                     /gene="pykF"
                     /locus_tag="OO179_002941"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5532924.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5311 a         4854 c         5174 g         4936 t
ORIGIN      
        1 ggttaaaaat acttttttat ttcggcgggg gagggtattt ccgccgaaat attattgctt
       61 atgcctgatg taaaatagtt aataccgtgc gcaaatcatt taccgagatt tgccctggcg
      121 cagaggcttt ttttaccgca ccaaaagttg ccgccgagcc aaatacttca ccaaccagac
      181 gagaaattac gccagttttt gccatcgaca tcgtgatgat tggacgatcg gcatactgct
      241 cctgcatctc cagggtcgcg gcaagcaacg tcagcacatc gctggtactt tgcggcatca
      301 gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt
      361 cttcggcttc cggcgtttta tggaagtcat ggttggacat gacaactttc acatcatgcg
      421 cgtgggcgta ggcgacggtt tctttgacct gatcatcgcc ggtaaataac tccagatcga
      481 tcatatcaac caggccgctg tcgatggctg cacgattgag agcaatataa gcctcggtgg
      541 aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt aaacagcagc ggtttttctg
      601 gcatggtttc acggagaatt tttgccgccg ccatgacaga ctccacattg gagaggtcgg
      661 caaagtggtc cacacgccat tccagaatat caaagtccgc ttcacgatag gcgagagctt
      721 cggatttcac gtgggcgata tctttcgcca tcagcgagac gatgatttta ggtgcgcccg
      781 caccaatgac gagatctttt acagttacgg ttttcatttt ttaccctttc tgcacgcggt
      841 cagcctgtca ggcaccgaac cccatgacct gtttaacata ttccagaggg aaatctttgc
      901 cagtccataa cgtgaactgt tcagcccctt gccacaacaa catgccgtat ccatcaatcg
      961 ttttgcaacc agcttgttgt gcctgctgca ataacttcgt catatgcggg ttatacacgc
     1021 attcagtgac cagcagtccc ggatgtaaca gactgatatc attaaccaat gattcattct
     1081 caaggggttt catacctact tttgtgccat tggttaaaat gtcggcggaa gccagggctt
     1141 cagcaaaggc ttgctgatcg gcgagatcgg tatccgtgac gacacaatcg gtgttttcgt
     1201 taacccgctg cgcgaaggcg agggctttat cgaagaactc atcccgacgg ttaaagagtt
     1261 taatttcttt taaaccttca attgccccct gcgcgccaat cgccgttgag gcacctccgg
     1321 cccctaacag caccatcgtt ttgcctttga tatcaaaacc gctctcttta atggcgcgaa
     1381 tatggcccgt gccgtcggtg ttatagccac gcagatagcc atcatcatta acgatggtgt
     1441 tgatggcacc caccagtttg gctgctggcg ttaattcatc aacatattca cacaccagtt
     1501 gtttgttcgg catcgacaca ccagttccgc gcattttgag ggcttttaat ccttcaattg
     1561 ctccgggaaa gctatcgtta tccacttcga aggccatata ggtaaatggc aatcccgctt
     1621 tttctaaggc tttattctgc atttcaggcg ataaactgtg gcggatagga taagccatca
     1681 atccaatcaa ttcgtatttt gcggtaacat ccataattaa ctcctttaac ctctatgctt
     1741 aattgtgttt aaccgggtgc taaatttgcg ttcgccaaac tgcacatcat tctcaggaat
     1801 aataaaaacg cggtaatagc ggctaaaaac aataattgcg gtaatcaggg ccagcagcgc
     1861 gaaagtaaaa tcgagaacaa tgatatattg caggccgatg ttcgacagat aaccggtaat
     1921 cagcggaata acaaagttag ccagtccacc catcatcata taaatactgg tgactttggc
     1981 tttgcttttg gggaaaaact ctgacatgac cgaaacaccg agctgtaaaa tgccgccagc
     2041 tgctgagaaa ccgataacaa aggcgccggc attacacacc agtggagaag ggtacaggta
     2101 gataatggca gctgttattg ttgccagcgc agagttaaat acattagccc agatgggccg
     2161 gaccattttt ttcagtagtg cggcaaaaat aaagacacag accaacgagc ccatactgta
     2221 ataagagatg gtttttaatg cctcagcttc tgacatacca gcaaaagcca tcgcatattt
     2281 gggcatccac accacaatca cataaaaggt cgagaatgcg gctacaccga acagtaccga
     2341 tgaaacacct tccagccaga ctaaaggttt gctgttcatt tgcggtaatt cattagctac
     2401 gctggcgtcc accaactggc tggggaattt gcttttcaac agcatcagcg tgatcagtac
     2461 aaacagaata cccggaataa tcagcccata gccgtaccag atattattga gcaacatata
     2521 gctcaccagc attgggtaga acatttgccc aaatgacacc atcgctttaa ccagtatgac
     2581 cgccgaacca gaggctttcg gaaagcattc catgagcgcg gggtagccac ccgtatccag
     2641 cgctgagtta gcgataccta cgcacactgc cagaccgtag gcgagagtta aattcgggca
     2701 agcgggaata ccaaagaaga atagcagata cattattact gccattaata tcaccgcccg
     2761 acgaccaaac ttatcggaga tcacaccgaa gaataaaata ctgatcaatc gccccaaacc
     2821 gataccggaa attaaatagg caatgcccgc gttgtcagtg gaaaactttt ctgccagaga
     2881 tgtcatattt tgggcaagcg taataacact aatgccgtgc aggaaatagc tgaagtaaat
     2941 acaaagaaca gccaggataa atggcgtgct gaaagataat ttcgttaccg tacgtattgt
     3001 tctccgacgg tttacataat tactatactg aagatgattt tccctgggta catggcataa
     3061 agtttatgtt tccaggaata ttgcatgtca tagtttgttt ttgatcgcga ttttccttag
     3121 ttagcgcaga aagagttatt tatccgtaag atcttgcatg acctgaaaag aatattgcat
     3181 tacccacccg gagcgacatt ttcctttagc gagtgatgcc gcgttttttt gcggctgcgt
     3241 aagccgataa acagtgccag taaaaaaccg atggcagtat cgaaccacat aatatcggca
     3301 atacttcttt gggacagatg agccgtaatc aacggaatag taaaggtcgc aatactgccc
     3361 gtactgtaat agatccctgt agctttacct ttagcgtaag ggaaacgttc agccattaac
     3421 gtcaggccaa tttgcacaac acctccagca gaggtaaaac caatgacaaa agcaaatatt
     3481 atcaccacat aaaatgtggg atgcaggcag acggtaaaca gagcaataaa tgagataaag
     3541 gtgtacagca tcagtaatgt tgttgggcga acggtattac gaatgagtgg agcggtaata
     3601 aatacacaaa gcagcgaacc cacggtgtag atactgagta gtttgatcga catagtgtat
     3661 gacatgcctg caacaaattg tccgtactgt gccagccact ggctaaccag ataaaacgtt
     3721 gccattgaga tatagccata taaggtataa ctggctaaat cgataattga acagcgatgt
     3781 tccgtagagc tggtggtttt ctttatgaca ggtaagcgac gacccggatg gggtgggaac
     3841 gtacaacggt ataaaaacag agcgttaata aacataatgc ctgcagcaat cataaaggac
     3901 caaccgaacc acagttcagc ccacactaac aggctaatga ttagcggtaa taaaaattgt
     3961 ccgctggaaa caaatgcttt aattaaaata ttggctgtcc caggtgagcg tggaaaagct
     4021 tccatcaaac tgggataagt gcctgcatcg agaaaactgt ttgccattcc cgccagaaag
     4081 ccaaaaacat aagcgataat gatgttattg gtctgcagga tgtcaaaaaa gaaggccata
     4141 tagcagcaca tcccgagcat gataaaaggg cggcgaccaa agcgatcgga taataatcct
     4201 gcaaaaagca agacacttaa tcgaccaatg cccagcgatg agataactat cgagacaccc
     4261 gcggcattag tctgccaaag tgtctccagc gaggccatat tcaggctcat caaaatgacg
     4321 cccataccat gcaccaggta attaaaatac aacccaagtg cggtagggta atagggattt
     4381 ttcataggtt ctacttagca aaggtgcttt aaaaagtgta gcgatgttgt gtcagtatcc
     4441 attcattatt gttgtaacaa cgacaattcc tttctgtgga aaaattaaca aagccgctcc
     4501 agatcgtgtg catataattc agcggctacc gattgataga tcttccagtc aataaaattg
     4561 ccatcagggt agacttgttg aaacgcggtt aagtcgggga aaaaacgcat ggtgttgttg
     4621 ccgatgcggt tatttatttt ctctatgatg gcatgaagat gtttttttcg ttgctgacgc
     4681 atagccaaca gttgctcgac tacacaatca ggaatggcgc atttgccatt ttcccactga
     4741 cgccagcttt cactgtcacc ggtctgggca atccaggtcg cgcattcatc aatagtcatg
     4801 gcaaaaatat ggcggagtgc ctggagttca taagcgttca tcgtgtaatg gctgcatgaa
     4861 ataaacaata aaatataaat aacattatca gcctgctgac ggcttgaaga gagtcacagt
     4921 atcttgtgca acattattgg aaagagtgag gggcgaggta gcgaagcgga aaaatgttaa
     4981 aaaaaagccc atcgtgggag atgggaaaag actacacaca gcaattcgtt gtttcactca
     5041 ggggatttcc atgcttataa atcaatatgt tgatttataa ccgtgagcta atagtaggca
     5101 tacggacttt ttgcgtcgat cagattcgtc tcattagttt aaaaactgta aagaatttgc
     5161 gacaggtact accagctgaa tggcggtaag caggaaaata tggggaagag caagcaaata
     5221 tcaattagcg gtgattaaca atcaggcggc agagccgcct gatgagaaat tacttattcg
     5281 gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg ttggtccgtc ggtggcggga
     5341 cttcttccac ccacgcggca aacagacgcc aggaaacggc taacagaacc ggaccaataa
     5401 acagaccgat catcccgaaa gcaatcaaac caccaataac gccagagagg atcaggatca
     5461 gcggtaaatc ggcacccatg cgaattaaca ttgggcggat gacgttatcc agcgtgccaa
     5521 ccacaccgct ccacactaac aataccgttc cccaggtggt atcgccggtc cagtagagcc
     5581 agataatcgc cggaatcagt accggcaacg ggccaagctg gacaaggcag gagaggatca
     5641 ttaacaccgt tagcaacgtt gcataaggta cgccggatac ggcgaggccg ataccgccaa
     5701 gcactgcctg tactaacgcc gtcaccacca cacccagcgc caccgcgcgg atagcctgtg
     5761 ccgccagcag cacggcggca tctccgcgaa cgcctgccag acgggttgca aaatggcgaa
     5821 tgccttgtgc cacctgttca ccgcgccagt acagcagggc actgaagaga agcatcagcg
     5881 cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc aacgaaccag gtggtggtgg
     5941 tgccaatata agggcggact ttcgccatga tcgccgtacc ccccatatcc agcaagttgt
     6001 gccagcctgc atacagcttc gcgccaatca ccggaatggt attaagccac gccagatcgg
     6061 gtaacgtcat gtcgccgctg gaaatggctt taattagcgg gccgctgccg tcgacgatac
     6121 tgttaaccag caaagcaata gggatgataa acaccatcac taataacagc gtcatcacca
     6181 gaacggcgag agagcggcgg ccaaacatga tcttttgcaa acgtaacaat accggccagg
     6241 tggcgataac caccgtaccg gcccatgcaa agccgagaat aaagggttga acaatccaca
     6301 gacatgccac aatcatgatg gctaaaaaca gcaccgaaag cagaatttgt gcgacatccc
     6361 tgggctgacg aacatttacc ataactactt ttcacctttt ttgtgcgtca aatcgtcgac
     6421 gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc aatttcagcg gtttttaaca
     6481 ggccgattct gcccttaatt ctgatgggcg tgttagaaaa aaatgtggta caacaactca
     6541 ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc atgtgctgcg gctacgttcg
     6601 ttcaccccag tcacttacta ttgtaagctc ctggagttca ctcgcttgcc gccttcctgc
     6661 aactcgaaat ccatagagta aataaacatc acctttcagt gcgctattgc agccgcctga
     6721 aagaatagtg tatcgaccta catcacagac cgcaggaaag ggtcaatata atgattccac
     6781 agatttccct ggcacccggt gtcgttcaac tggtgcttaa ttttttgcaa gagctggagc
     6841 aacaaggttt taccggcgat gcggcgacaa gttatgccga tcgtctgaca atgtcgaccg
     6901 acaacagtat ttaccaactt ctccccgatg cggtggtatt tccgcgttca accgcagatg
     6961 tggcgctgat cgcccgtctt gccgcgcagg aacgctattc atcgctgatc tttacccccc
     7021 gcggcggcgg caccggcact aacggtcagg cgctcaacca ggggattatt gttgatatgt
     7081 cccgccatat gaaccgcatc atcgaaatta accctgaaga gggctgggtg cgcgttgagg
     7141 ccggggtgat aaaagaccaa ctcaatcagt acctgaaacc gttcggctac ttttttgcgc
     7201 cggaactttc gaccagcaac cgggcaacgc tcggtgggat gatcaatacc gatgcatccg
     7261 gtcagggatc gctggtctat ggcaaaacgt cagatcacgt acttggcgta cgcgcggtgt
     7321 tgttgggggg cgatattctc gatacgcaac ctttacccgt cgagctggcg gaaacgctgg
     7381 gtaaatccaa taccacaatc gggcgaattt ataacacggt ttatcaacgt tgccgtcagc
     7441 aacgccagtt aatcatcgac aacttcccca aacttaaccg ctttcttacc ggttacgatc
     7501 tgcgtcatgt ctttaacgat gagatgaccg agttcgacct gacgcgcatt ctgacgggtt
     7561 cagaagggac gctggccttt attaccgaag cgcggctgga tattacgcgc ttgcctaaag
     7621 tgcgccgtct ggtgaatgtc aaatatgact cttttgactc tgcgctgcgt aacgcgccgt
     7681 ttatggttga ggcgcgggcg ctttcggtag agacggtgga ctcaaaagtg ctgaatctgg
     7741 cgcgggaaga tattgtctgg cattccgtca gcgagttgat taccgatgtg cctgacaaag
     7801 agatgctcgg actgaacatt gtggaatttg ctggtgatga tgaggcgctg attgacgagc
     7861 gagttaatgc actctgtgtg cggcttgatg agctgattgc cagccaacag gcaggtgtga
     7921 ttggctggca ggtgtgccgc gagctggcgg gggttgaagg tatctatgcg atgcgcaaaa
     7981 aagccgttgg tctgcttggc aatgccaaag gtgccgctaa gccaattccg tttgctgagg
     8041 atacctgcgt accgccggaa catctggccg attatattgc tgaatttcgc gcgctgctcg
     8101 acagccacgg cttaagctac ggtatgttcg gtcacgtcga cgcaggtgtc ttgcacgtcc
     8161 gtccggcact ggatatgtgc gatccgcaac aagagatttt gatgaagcaa atctctgatg
     8221 acgtggtggc gctgactgcg aaatacggtg gtttgttgtg gggcgagcac ggcaaaggtt
     8281 ttcgcgctga atacagcccg gcgtttttcg gtgaggaact ttttgcagaa ctgcgcaaag
     8341 tgaaagcggc atttgacccg cataaccgac tcaacccagg gaagatttgc ccgccagaag
     8401 gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg cggtacattc gatcggcaga
     8461 tccccattgc ggtacgccag cagtggcgcg gtgcgatgga gtgtaacggc aacggtttat
     8521 gcttcaactt tgatgcccgt agtccgatgt gtccgtcgat gaagatcacc cagaaccgga
     8581 ttcattcacc gaaagggcgc gcaacgctgg tgcgtgaatg gctgcgcttg ttggcggatc
     8641 gcggcgttga cccactcaaa ctggaacaag aactgcctga atccggcgtc agtttgcgga
     8701 cgttaattgc ccgcacgcgc aatagctggc atgcgaataa aggcgaatat gacttctcac
     8761 acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa agcttgttcg acccagtgcc
     8821 ccatcaaaat tgatgtgccg gagtttcgct ctcgttttct gcagctctat cacacccgtt
     8881 atttacgccc gcttcgcgac cacctcgttg ctacggtcga gagctacgca ccgctgatgg
     8941 cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc aaactctcgg
     9001 aaaaacatat cggcatggtt gatttgccac tgctgtcggt cccctcgcta caacaacaaa
     9061 tggtggggca tcgctcggca aacatgacgc tggaacagct tgaagctctc aatgcagagc
     9121 agaaagcgcg cacggtgttg gtggtgcagg acccctttac cagctattac gatgcgcaag
     9181 tggtggcgga ttttgtccgt ctggttgaaa aattaggttt ccagcctgtg ttactgccat
     9241 tttcgccaaa tggcaaagcc cagcatatca aaggctttct taatcgtttt gcgaagacgg
     9301 cgaaaaagac ggcggatttc ctcaaccgta tggctaagct tgggatgcca atggtgggcg
     9361 tcgatccggc gctggtactt tgttatcgcg atgaatataa actggcgctg ggcgaggaac
     9421 gtggcgagtt taacgtctta cttgcgaatg aatggctggc aagcgcactt gagtcacatc
     9481 cagtggctac agtcagcggt gaatcatggt atttctttgg tcactgtacc gaggttaccg
     9541 ctttgccggg cgcgccagca caatgggccg cgatatttgc ccgttttggc gcgaaactgg
     9601 aaaatgtcag cgtgggttgc tgcggcatgg cagggactta cggacatgaa gcgaaaaacc
     9661 atgaaaattc gctcgggatc tatgagttat cctggcatca ggctatgcag cgactgccgc
     9721 gtaaccgctg tctggcgacc ggatattcct gccgtagcca ggtaaaacgg gttgaaggca
     9781 cgggggtacg ccatcctgtg caggctttac tggagattat taaatgatat ggaaacggaa
     9841 aatcaccctg gaagcactga atgctatggg tgaaggaaac atggtggggt tcctggatat
     9901 tcgctttgaa catattggtg atgacaccct tgaagcgaca atgccagtag actcgcggac
     9961 aaagcagcct ttcgggttgc tgcatggagg agcatccgtg gtactggccg aaagtatcgg
    10021 ttccgttgcc ggttatttat gtaccgaagg tgagcaaaaa gtggttggtc tggaaatcaa
    10081 tgctaatcac gtccgctcgg cacgagaagg gcgggtgcgc ggcatatgta aaccgttgca
    10141 tctcggttcg cgtcaccagg tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg
    10201 ctgttcgtca cgattgacga ctgccatttt gtgacgttgt caccagaaaa gtgtgacgca
    10261 tattgctttt ggtcattatc tgcacagtaa acggtggtat actgtgcagg tcttgtctaa
    10321 aagcgaggat gctatgtcta ctcaacttga tcccacccag ctggccattg aatttttacg
    10381 ccgtgaccag agcaatcttt cacctgccca atatctcaaa cgtctgaaac aactagaact
    10441 tgaatttgct gacctgctta ctctctcttc tgctgaatta aaagaagaaa tctactttgc
    10501 ctggcgtctg ggcgtgcatt gatcctgact taatgccgca agttcgccaa atcgacctgg
    10561 attaacctcc gtccaggttc gatgaccttc ttaatactgc ttatctaccg cctgatattg
    10621 ttcaaaaata atactaatcg cagtttgtgt taaaacggcg ggttagcttt atgagattat
    10681 tcttatcgcc ccttcaagag ctaagccact gagagtgccg gagataagcg ccggatgggg
    10741 tagaaaccct taagcctgtg tcgcacagac ttaagggttt tcttatttct gaacgctgca
    10801 cttttttacc tatctcttaa atagtcattt tcaatacaac tttttctttt tcatcacagc
    10861 cttaatgctc cttagataat aaccattatc taacaatgag atacctaatt cttagcgtgc
    10921 ctgttaaccc acacatcagg ggctatgctt attaaaagca ggcaaaaaga atgttttacg
    10981 gtaaagcccc tgcgtttgct gggttgaact gataatcatt atcactaaca tgctgttata
    11041 cgctgaaagc gatgaagtga ggtaaataga tggacatgca ttcaggaacc tttaacccac
    11101 aggatttcgc ctggcaaggc ttaacgctga cacccgcagc ggcgatacac atccgcgagc
    11161 tggtggcaaa gcagcctggt atggtcggcg tgcgcttagg tgtgaagcaa acgggctgcg
    11221 cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat ctgctgtttg
    11281 aacacgacgg cgcgaagctg tttgtcccgc tgcaatcgat gccgtttatt gatggcacgg
    11341 aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac cctaaagccc
    11401 agaatgaatg tggctgtggc gaaagcttta gggtataggc ggtactatgt ctcgtaatac
    11461 tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata aagaaggatt
    11521 cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg tggtgcgcgc
    11581 aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctaa acgcctatcg
    11641 cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca agctgaatta
    11701 tcaggattac agctactact cagcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc
    11761 tgaacctggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag aggtggaggc
    11821 ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat
    11881 tttcgactct gtttcggttg ccaccactta tcgcgaaaaa ctggcggagc agggaattat
    11941 tttctgttcc tttggtgagg cgatccacga tcacccggaa ctggtgcgta aatatctcgg
    12001 caccgtggtg ccggggaatg acaacttctt tgcagcactt aatgcggcgg tagcctctga
    12061 cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg atggaacttt ccacctattt
    12121 tcgcattaac gcggaaaaaa ccgggcagtt tgagcgcaca attctggtgg ccgacgaaga
    12181 cagctacgtc agctacattg aaggctgttc cgctccggtg cgtgacagct atcagttgca
    12241 cgcggcagtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt ccacggtaca
    12301 aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca ccaagcgtgc
    12361 tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg ggtcagcgat
    12421 tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg agttttactc
    12481 agtggcgctg accagcggtc atcagcaagc ggataccggc accaagatga tccacatcgg
    12541 taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata gtcagaacag
    12601 ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg
    12661 cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg
    12721 caataacagc gcacaactgg agcacgaggc aacgacatca cgtattggtg aagatcaact
    12781 gttttactgc ctgcaacgcg ggatcagcga agaagacgcc atctcgatga ttgttaacgg
    12841 tttctgcaaa gacgtgttct cagaactgcc gctggaattt gccgttgaag cacaaaaact
    12901 cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa gtattaaaga
    12961 tttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg acgttcgtcc
    13021 cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct tatcggcaac
    13081 gcttgccggg cgcgaagatt atgaagtgac gggcggcacg gttgagttca aaggcaaaga
    13141 tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc atctttatgg ccttccagta
    13201 tccggtggag attccaggcg tcagtaacca gtttttcctg caaacggcac ttaatgcagt
    13261 gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt tgatggaaga
    13321 gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa acgttggttt
    13381 ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg aaccggagtt
    13441 atgcattctt gatgagtcgg actccgggct ggatattgac gcattaaaag tggtcgccga
    13501 tggcgtgaac tcgctgcgtg atggcaagcg ctcattcatc attgttacgc actaccaacg
    13561 cattctcgac tacatcaagc ctgattacgt tcatgtgcta tatcagggac gaattgtgaa
    13621 atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct ggcttaccga
    13681 acagcagtaa tgcgctgcaa cagtggcatc acttgtttga agctgaaggg acaaaacgct
    13741 caccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg acacgtaaac
    13801 atgaaaactg gaaatatacg ccgctggaag ggctgaccaa tagtcagttt gtcagcattg
    13861 cgggagatat atccccacag cagcgtgatt ccttagcgtt aacgttagac gccgtgcggc
    13921 tggtgtttgt cgatggacgt tacgtgcccg cgctgagcga tgcaattgaa ggcagcggat
    13981 atgaagtgag cattaacgac gaccgtcagg ggttacccga cgctattcag gcggaagtgt
    14041 ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc
    14101 aacggccggc aaagccattg ctgttaatgc atatcaccca gggcgtggca ggtgaagagg
    14161 tgaacactgc ccattaccgg catcatctgg atctggcgga aggtgccgaa gcaacggtga
    14221 tcgaacattt tgtcagcctt aatgatgctc gtcattttac cggggcacgg ttcactatca
    14281 atgtcgcagc gaacgcccac ttgcagcata tcaagctggc gtttgaaaac ccggtcagtc
    14341 accactttgc ccataacgat ttgttgctgg ctgacgatgc caccgcattt agccatagtt
    14401 tcctgctggg tggcgcagtg ttacgacata acaccagtac gcaactcaat ggcgaaaaca
    14461 gcacgctgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt gatacccgta
    14521 cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa actatcgtca
    14581 gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa
    14641 cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcggaa gtggatacaa
    14701 aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg acggtggggc
    14761 gtattgatga tgaacagatg ttctatctgc gctcgcgcgg gatcaatcag caggatgccc
    14821 agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt gatgaggggc
    14881 ttaaacagca ggtgctggcc cgaatcggtc aacggctgcc aggaggtgca agatgacttt
    14941 ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg cgtgaggtaa acggtttgcc
    15001 gctggcttat ctcgacagcg ccgccagtgc acagaaaccg agccaggtga ttgacgccga
    15061 ggccgagttt tatcgtcatg gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc
    15121 ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta ttaatgcccg
    15181 ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa
    15241 tagctggggc aacagcaatg tgcgggcggg cgataacatc atcatcagtc agatggagca
    15301 ccacgctaac attgtcccct ggcagatgct ttgcgcacgc gttggcgcag agctgcgtgt
    15361 gatccctctc aaccccgacg gtacgctgca actggagacg ctgcctacgc tgtttgatga
    15421 gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttggcacag aaaatccact
    15481 ggcggaaatg atcacgcttg cgcaccagca tggcgcaaaa gtgctggtgg atggcgctca
    15541 ggcggtgatg catcatccgg tggatgttca ggcgctggat tgcgactttt acgtgttctc
    15601 cgggcataaa ctgtatggcc ccaccggaat tggcattctt tatgtgaaag aagccttgtt
    15661 gcaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca gcctgagtga
    15721 aggcactacc tggaccaaag caccatggcg gtttgaagcc ggtacaccca ataccggggg
    15781 catcattggt cttggcgcgg cgctggagta tgtttcggcg ctggggctta atagcatagc
    15841 cgagtatgaa cagaatctga tgcattatgc gctatcacag ctggaatctg taccggatct
    15901 cactctctat ggccaacaaa acaggcttgg cgttattgct tttaatctcg gtaaacacca
    15961 cgcctatgat gttggcagtt ttctcgataa ttacggcatt gctgtgcgta ccggacatca
    16021 ctgcgctatg ccattaatgg cctattacaa cgtccctgcg atgtgtcggg cgtcgctggc
    16081 catgtataac acccatgaag aagtggagcg tctggtgacc ggcctgcaac gtattcaccg
    16141 tctgctggga taacagggag gcactatggc tttattgccg gataaagaaa aattgctgcg
    16201 taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg agctgggcca
    16261 gcgtctgcca gaattacgcg ccgaagacgg aagcccacaa aatagcattc agggctgcca
    16321 gagtcaggtg tggattgtca tgcgccagaa tgcgcaggga attattgaat tgcagggcga
    16381 cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc tttattctct acgatcagat
    16441 gacgccgcag gatattgtca atttcgatgt gcgtccgtgg tttgaaaaaa tggcgctcac
    16501 ccaacatctc accccatctc gttcacaagg tctggaagcg atgattcgcg caattcgcgc
    16561 caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg ctcacattgc
    16621 cggatggtca atgtttacac tgacctgcac aggagtgttt tggtttcagg tgaacataag
    16681 caccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca atgaaacgcg
    16741 cgtctttgct tacactcacg cttatcggcg cttttagcgc catccaggct gcctgggcgg
    16801 ttgattatcc gctaccacca accggaagcc gactggttgg gcaaaatcaa acgtatacgg
    16861 tgcaagaagg ggataaaaac cttcaggcta ttgcccgacg ttttgatact gcagcaatgt
    16921 tgatccttga agccaataac actatcgccc cggtgccaaa acctggtacg acgataacta
    16981 ttccttcaca actgttatta cctgatgcac cgcgtcaggg gattatcgtt aaccttgcag
    17041 agctgcgcct ttattattat ccgccgggag aaaatattgt gcaggtttat ccaataggta
    17101 ttggattgca ggggctggaa acgccggtga tggaaacgcg tgttgggcag aaaatcccta
    17161 acccaacctg gacgcctacg gcaggcattc gtcagcgttc gctggagcgt ggcattaaat
    17221 taccgccagt cgttcctgcc ggaccaaata acccgctagg acgttacgca ctgcgcctcg
    17281 cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc gccggacagc gttggtttgc
    17341 gcgtcagttc agggtgtatt cgcatgaatg caccggatat taaagccttg ttctccagcg
    17401 tgcggacggg aacgccggtg aaagtgatca acgaaccggt gaaatattcc gtggaaccta
    17461 acgggatgcg ttatgttgaa gtacatcgac cactatcagc agaagaacag caaaacgttc
    17521 agacaatgcc atacacactg ccagcaggct ttacgcaatt taaagacaat aaggctgtag
    17581 atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt tcggtgagca
    17641 gtggagcaac tcccgcagcc agcaatgcgc cttcagtaga gtcagcgcag aatggtgaac
    17701 cagagcaagg gaatatgtta cgcgcgacgc agtagtggta aatagcagac aaaaaaatgg
    17761 cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat ttagtagcca
    17821 tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga acgtcggaac
    17881 gcattgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc tgaacgtcag
    17941 aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta gaacccagga
    18001 ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag attgagttaa
    18061 tctccatgta gcgttacaag tattacacaa agttttttat gttgagaata tttttttgat
    18121 gggaaggcac ttatttttga tcgttcgctc aaagaagcat cgaacgctgg gttttagata
    18181 aaaaaattga gagaaaacag gatgcttcca tcggattcat cttaaataaa gtgagattat
    18241 atagtgaaat ccgatttgat tttttattgc ttctggttat cgattaaata aaaaaagcgc
    18301 ccatcagggc gcttcgatat acaaattaat tcacaaaagc aatattacag gacgtgaaca
    18361 gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag aaaccataac tacgacgtca
    18421 cctttgtgtg ccagaccgct ctgcagagcc agttctttac ccagacggta gaaatcatca
    18481 gtagaagtga tctctttaac cagctgcggc acaacgcctt tgctcagtac caactgatga
    18541 gccgtttttt cgttggtggt cagtgccagg atggtggcat ccgggaagta tttacgtact
    18601 gcgcgagcag atttaccgcc ctgggtagca accacgatca gcggagcatc cagtttttca
    18661 gcagtttcaa cggcaccacg gcataccgct tcggtgatgc gcagtttacg gttgtcattg
    18721 ttgaactcga gacggctgtt catcacgcgg tcggtacgtt cgcagatggt cgccatgata
    18781 gaaaccgctt ccagcgggta tttacctttt gcggattcac cagacagcat cactgcgtca
    18841 gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt
    18901 ttgatcatgg aatccagcat ctgggtcgca gtgataacga ctttacgtgc acggatacat
    18961 ttttcgatca tcatcttctg ggcgaagata acttcttcta ccgggatttc tacacccagg
    19021 tcgccacgcg caaccatgat gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg
    19081 ccttcctggt tttcgatttt ggagatgatg tggatgtttt cgccgccgtg cgctttcagg
    19141 tgctcacgga tttcgataac gtcagaacgc ttacgaataa aggaagcagc aacaaagtct
    19201 acgccttttt cgcaaccaaa gatcaggtcc tgtttgtctt tttcagccag tgctggcaga
    19261 gcaatggaaa cgccaggcag gttcacacct ttgttttcgc ccaggtcacc gttgttcagc
    19321 actttacaga taactttgtt accttcaatg gcggtaactt ccataccgat cagaccatcg
    19381 tcaaccagta cggtgttgcc aacagacagg tcagtagtga aaccttcata cgttaccgca
    19441 accatttcgc tgttgccgat aacagattta tcagtggtga aagtaaaggt ctgaccagct
    19501 ttcagagaaa catcgttacc gccttccagt ttcatggtgc ggatttccgg acctttggta
    19561 tcaagcagga tagcggcggt tttaccagtt ttgctcatca cgttgcgcag attctgaatg
    19621 cgctgaccgt gttctgcata gtcaccatga gagaagttca gacgcataac gttcatgcca
    19681 gcgtccagca ttttagctaa catctcttca gattcggttt tcggtccgat ggtgcaaaca
    19741 attttggtct ttttcatgac agtcttagtc tttaagttga gaaggatggg agaaacttgc
    19801 tttctgggcg cactgccgga aagcgaatcc ggtattacga aagtggtggt gcctcgctct
    19861 aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg
    19921 tgagtgtctg tttttgagtc gttgagttct acagtccaaa gtgctgaaac cattcaagag
    19981 tcaattggcg cgcattatac gctgaaatta atccaaaagg aaaacaaaaa cagcgtttca
    20041 aaaaatatac aacttgttag acaataaatt gttatcaaag atttaacgtt ccagggaaaa
    20101 ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc cgaagttgtc agtgtgagga
    20161 taagtgaaag ttcatccggt gtataaatat atcatcgctc aattgctaca gttaaaataa
    20221 tttatcgcgt tatttgtctg attttgtttg atttttgttt aattggttag attga
//
LOCUS       DAJTNW010000079        20219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNW010000079 DAJTNW010000000
VERSION     DAJTNW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="OO179_002942"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="OO179_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="OO179_002943"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="OO179_002943"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5532925.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="OO179_002944"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="OO179_002944"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5532926.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="OO179_002945"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="OO179_002945"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5532927.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="OO179_002946"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="OO179_002946"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5532928.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="OO179_002947"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="OO179_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5532929.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="OO179_002948"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="OO179_002948"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5532930.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="OO179_002949"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="OO179_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5532931.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="OO179_002950"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="OO179_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5532932.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="OO179_002951"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="OO179_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5532933.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="OO179_002952"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="OO179_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5532934.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="OO179_002953"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="OO179_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5532935.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="OO179_002954"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="OO179_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5532936.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="OO179_002955"
     CDS             complement(12654..13094)
                     /locus_tag="OO179_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5532937.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="OO179_002956"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="OO179_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5532938.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="OO179_002957"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="OO179_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5532939.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="OO179_002958"
     CDS             14036..15076
                     /locus_tag="OO179_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5532940.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="OO179_002959"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="OO179_002959"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5532941.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="OO179_002960"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="OO179_002960"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5532942.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="OO179_002961"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="OO179_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5532943.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="OO179_002962"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="OO179_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5532944.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5140 a         5120 c         5201 g         4758 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatacttgg
//
LOCUS       DAJTNW010000080        19414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNW010000080 DAJTNW010000000
VERSION     DAJTNW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="OO179_002963"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="OO179_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="OO179_002964"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="OO179_002964"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5532945.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO179_002965"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO179_002965"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5532946.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="OO179_002966"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="OO179_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5532947.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="OO179_002967"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="OO179_002967"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5532948.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="OO179_002968"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="OO179_002968"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5532949.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="OO179_002969"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="OO179_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5532950.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="OO179_002970"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="OO179_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5532951.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="OO179_002971"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="OO179_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5532952.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="OO179_002972"
     CDS             11402..11617
                     /locus_tag="OO179_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5532953.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO179_002973"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO179_002973"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5532954.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="OO179_002974"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="OO179_002974"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5532955.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="OO179_002975"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="OO179_002975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5532956.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="OO179_002976"
     CDS             complement(16353..16892)
                     /locus_tag="OO179_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5532957.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="OO179_002977"
     CDS             complement(16908..17426)
                     /locus_tag="OO179_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5532958.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="OO179_002978"
     CDS             complement(17737..17928)
                     /locus_tag="OO179_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5532959.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="OO179_002979"
     CDS             17946..18098
                     /locus_tag="OO179_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532960.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO179_002980"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO179_002980"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5532961.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5278 g         4839 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAJTNW010000081        19263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNW010000081 DAJTNW010000000
VERSION     DAJTNW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            32..331
                     /locus_tag="OO179_002981"
     CDS             32..331
                     /locus_tag="OO179_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5532962.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="OO179_002982"
     CDS             328..2076
                     /locus_tag="OO179_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5532963.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="OO179_002983"
     CDS             2365..2643
                     /locus_tag="OO179_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532964.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="OO179_002984"
     CDS             2640..2945
                     /locus_tag="OO179_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532965.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="OO179_002985"
     CDS             2955..3116
                     /locus_tag="OO179_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5532966.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="OO179_002986"
     CDS             3258..3482
                     /locus_tag="OO179_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532967.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="OO179_002987"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="OO179_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="OO179_002988"
     CDS             4980..5552
                     /locus_tag="OO179_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5532968.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="OO179_002989"
     CDS             5554..6765
                     /locus_tag="OO179_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5532969.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="OO179_002990"
     CDS             6762..7100
                     /locus_tag="OO179_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5532970.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="OO179_002991"
     CDS             7097..7393
                     /locus_tag="OO179_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5532971.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="OO179_002992"
     CDS             7393..7833
                     /locus_tag="OO179_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5532972.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="OO179_002993"
     CDS             7817..7999
                     /locus_tag="OO179_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532973.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="OO179_002994"
     CDS             8055..8345
                     /locus_tag="OO179_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5532974.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="OO179_002995"
     CDS             8365..10026
                     /locus_tag="OO179_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5532975.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="OO179_002996"
     CDS             10040..10321
                     /locus_tag="OO179_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5532976.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="OO179_002997"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="OO179_002997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5532977.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="OO179_002998"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="OO179_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5532978.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="OO179_002999"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="OO179_002999"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5532979.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="OO179_003000"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="OO179_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5532980.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="OO179_003001"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="OO179_003001"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5532981.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="OO179_003002"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="OO179_003002"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5532982.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="OO179_003003"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="OO179_003003"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5532983.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="OO179_003004"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="OO179_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5532984.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5159 g         4360 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 tat
//
LOCUS       DAJTNW010000082        19043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNW010000082 DAJTNW010000000
VERSION     DAJTNW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..294
                     /locus_tag="OO179_003005"
     CDS             <1..294
                     /locus_tag="OO179_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5532985.1"
                     /translation="RWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTL
                     DSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            266..2194
                     /locus_tag="OO179_003006"
     CDS             266..2194
                     /locus_tag="OO179_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5532986.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2178..2381
                     /locus_tag="OO179_003007"
     CDS             2178..2381
                     /locus_tag="OO179_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5532987.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2378..3971
                     /locus_tag="OO179_003008"
                     /pseudo
     CDS             2378..3971
                     /locus_tag="OO179_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3961..5466
                     /locus_tag="OO179_003009"
     CDS             3961..5466
                     /locus_tag="OO179_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5532988.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5503..5850
                     /locus_tag="OO179_003010"
     CDS             5503..5850
                     /locus_tag="OO179_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5532989.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5908..6936
                     /locus_tag="OO179_003011"
     CDS             5908..6936
                     /locus_tag="OO179_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5532990.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6988..7374
                     /locus_tag="OO179_003012"
     CDS             6988..7374
                     /locus_tag="OO179_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5532991.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7386..7763
                     /locus_tag="OO179_003013"
     CDS             7386..7763
                     /locus_tag="OO179_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5532992.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7750..8334
                     /locus_tag="OO179_003014"
     CDS             7750..8334
                     /locus_tag="OO179_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5532993.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8331..8732
                     /locus_tag="OO179_003015"
     CDS             8331..8732
                     /locus_tag="OO179_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5532994.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8743..9483
                     /locus_tag="OO179_003016"
     CDS             8743..9483
                     /locus_tag="OO179_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5532995.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9542..9928
                     /locus_tag="OO179_003017"
     CDS             9542..9928
                     /locus_tag="OO179_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5532996.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9937..10266
                     /locus_tag="OO179_003018"
     CDS             9937..10266
                     /locus_tag="OO179_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5532997.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10238..12976
                     /locus_tag="OO179_003019"
     CDS             10238..12976
                     /locus_tag="OO179_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5532998.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12976..13305
                     /locus_tag="OO179_003020"
     CDS             12976..13305
                     /locus_tag="OO179_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5532999.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13305..14003
                     /locus_tag="OO179_003021"
     CDS             13305..14003
                     /locus_tag="OO179_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5533000.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14008..14751
                     /locus_tag="OO179_003022"
     CDS             14008..14751
                     /locus_tag="OO179_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5533001.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14688..15290
                     /locus_tag="OO179_003023"
     CDS             14688..15290
                     /locus_tag="OO179_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5533002.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15351..18831
                     /locus_tag="OO179_003024"
                     /pseudo
     CDS             15351..18831
                     /locus_tag="OO179_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            18899..>19043
                     /locus_tag="OO179_003025"
     CDS             18899..>19043
                     /locus_tag="OO179_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5533003.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDL"
BASE COUNT         4450 a         4501 c         5998 g         4094 t
ORIGIN      
        1 agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca ggtgtcaaaa
       61 ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc gatggcttta
      121 tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga tttaacgccg
      181 cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg cgcgcgtgcg
      241 ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga gtaatatgat
      301 ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga cggctgtaga
      361 atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg gggaatggaa
      421 aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga ttcgtacagt
      481 taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg tgatcgggta
      541 ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact cagctgctga
      601 agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc tgaaaaaact
      661 ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc gtttttcctc
      721 tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg aaaaatctgt
      781 cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa aagaaggttc
      841 gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat ccatacgcgg
      901 ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg aatctgcgca
      961 tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc tgaagtttgg
     1021 cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa cggtgtatta
     1081 cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga ccggggggcg
     1141 ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt tcagtgcttc
     1201 gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg cgtacagtcc
     1261 gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag atcccaacgg
     1321 cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
     1381 actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg tgcctgtccg
     1441 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1501 ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca tgggacgtcc
     1561 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat accgccatgc
     1621 agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga tcgatggcga
     1681 aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg taaaaggtgc
     1741 ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc ggggtgtgta
     1801 tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa tgaaggccga
     1861 tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata atccggagat
     1921 tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa agtgggaaaa
     1981 aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     2041 ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt
     2101 gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac tggcagcgaa
     2161 gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc
     2221 ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc aggatcgaat
     2281 atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag tcggttctgg
     2341 gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc ctgtcctgat
     2401 tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg gcgcaggatt
     2461 tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg ccctgttgcc
     2521 tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg gtatcgcggc
     2581 taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta tcagctaccg
     2641 tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg tcggggaggt
     2701 ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg tggaagggaa
     2761 acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta acggcgaaat
     2821 ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc gttttaaagc
     2881 cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact ttctgcgggc
     2941 cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg aggatgattt
     3001 tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca ccgggcgtcc
     3061 ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg ccaatcagtt
     3121 ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc agcttcagtc
     3181 ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata ccgaaaaggc
     3241 atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta ttaatattct
     3301 ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg ttaaaattcc
     3361 gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca gacaatggat
     3421 tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc gtttcctacg
     3481 aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc tccgccaatg
     3541 agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg gccacgcaga
     3601 tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg ccacgggcgc
     3661 gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt ggctccggaa
     3721 gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt gaggccggac
     3781 tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac attttccgcc
     3841 agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg gtgtggatag
     3901 cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag acaacatcac
     3961 gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct gcttctggag
     4021 cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc agcaagcctt
     4081 tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga aacggacgag
     4141 tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg tattgctgta
     4201 ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt ttccggaatg
     4261 actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac tcaggtacgg
     4321 ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt tgactgcgct
     4381 gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa tgacacggcc
     4441 tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac ccagacatcc
     4501 cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca tctggcacag
     4561 gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg caatcagttt
     4621 gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc ggcgcgccgg
     4681 atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt tatgggaaca
     4741 gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga tgaattaatc
     4801 aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga tacaggaggc
     4861 actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg agtgatgggg
     4921 atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct ggcagggcaa
     4981 cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc gcaacaatct
     5041 ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc gaaaggacgt
     5101 gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa agcccgtccg
     5161 attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca gattatggcc
     5221 ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg tccggggatg
     5281 accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga accggtttct
     5341 gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc agcggtacag
     5401 ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct catggccatg
     5461 ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac gaaaaccatc
     5521 actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca taccgccaca
     5581 ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct ggatgaggat
     5641 accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg catactggta
     5701 ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac ctttgcgacg
     5761 gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc ctttgccggc
     5821 agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg gtagcctgac
     5881 tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca gttactcggt
     5941 tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt ccgccgtacg
     6001 gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac gccggtggcg
     6061 gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg tgaaactcgt
     6121 gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca ggcggtggag
     6181 cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg cctgcgtatc
     6241 atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga gatgcaggcg
     6301 gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga aactgttgag
     6361 gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac ggagtggagc
     6421 gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg tgattttgct
     6481 tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct gaatggcttt
     6541 aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct ggaaacggca
     6601 gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct ggcaatcgtg
     6661 gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct gccggacggc
     6721 acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc cattcaggat
     6781 gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca ctggctgacc
     6841 gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat ggtgctgccg
     6901 gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag ctaaatgctc
     6961 ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct gaaacgtctt
     7021 tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc ggcagatatt
     7081 gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt cgcagagccg
     7141 gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc agttcctgaa
     7201 cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc actggcgaca
     7261 ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc gtttcgtgtt
     7321 tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca gtaacaggag
     7381 atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc aatgaagtca
     7441 ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag gggacacacc
     7501 tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg atccggcttg
     7561 aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta cgccgtcagg
     7621 atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg gatgacgggg
     7681 gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat gccagaatga
     7741 ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct cgatacccgc
     7801 atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat tgtttctgtc
     7861 gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa aggtattccc
     7921 ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg aaaaatgtat
     7981 gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac cgcacaggtc
     8041 agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt actgaaagtg
     8101 gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg ctggcatgtg
     8161 atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat cccgatgtcc
     8221 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
     8281 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
     8341 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
     8401 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
     8461 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
     8521 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
     8581 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
     8641 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
     8701 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
     8761 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
     8821 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
     8881 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
     8941 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
     9001 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
     9061 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
     9121 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
     9181 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     9241 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     9301 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca gactgcttcg
     9361 gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatc
     9421 gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt cagcgcacca
     9481 taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc cggagaacat
     9541 gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     9601 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     9661 tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc gtatgggaat
     9721 ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt ctcaggatgt
     9781 tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg gcgcgggggc
     9841 ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg atgatgatca
     9901 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     9961 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc cactgagctt
    10021 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
    10081 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga tgctgcggtg
    10141 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
    10201 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
    10261 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
    10321 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
    10381 gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg caacagcagc
    10441 atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca tggcccgtga
    10501 ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg aggaacaggc
    10561 gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat
    10621 ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag cccggcggga
    10681 gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga ggggatccgg
    10741 gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga aaggcatgat
    10801 gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta aagcctggca
    10861 tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg gacattatgc
    10921 cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga atggtatcac
    10981 gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat ttcatggaaa
    11041 cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg gccagtcggt
    11101 cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc
    11161 gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac gcgtccttgg
    11221 ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg cagaggaaac
    11281 cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta cgctgcaaac
    11341 cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc gtgaagactc
    11401 gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa aatatccgtg
    11461 gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc ttcaggagaa
    11521 aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt accaggagca
    11581 acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa cggaagctgc
    11641 acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc aggctgtcag
    11701 ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga aaaatacacg
    11761 ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc agcaggcaca
    11821 ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg aaaggatgac
    11881 tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg acgggaaaaa
    11941 gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc aggcgctgac
    12001 gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg acctgagaaa
    12061 aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc gtgagcagca
    12121 caatctggat attgccactg caggtatggg ggataagcag cggcagcgct accaggcaca
    12181 gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg acagtcgcca
    12241 gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga aggggagcct
    12301 gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg cgcagggcga
    12361 ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg ataatgcggc
    12421 gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg cactggcgac
    12481 attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt tgtcagatat
    12541 ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg gttctttatt
    12601 cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga gtcgctacag
    12661 tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg
    12721 ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta agctgggggt
    12781 tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca acattgagat
    12841 caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg tttatgacct
    12901 cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg gccggttaag
    12961 tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat gaatgtggta
    13021 tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg
    13081 ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa aggtgaacac
    13141 ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca
    13201 ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg
    13261 ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag gatatccggc
    13321 aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa
    13381 tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag aacgaaaaag
    13441 gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag gggtcggggt
    13501 ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct aacctgcacg
    13561 gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg gtggtccggc
    13621 gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agcgacgccg
    13681 atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg
    13741 tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt ttcccggggc
    13801 gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg
    13861 gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag gacaaatgca
    13921 gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc ggtttccttt
    13981 ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat tctggcgcac
    14041 gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc ggagggagac
    14101 aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg gatggctccg
    14161 gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca cagtcatccc
    14221 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
    14281 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca tctcaccggg
    14341 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ttatcatctg
    14401 gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca cggtcagaat
    14461 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa
    14521 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
    14581 tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg agagaggtat
    14641 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg gcacgcatct
    14701 gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
    14761 ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg
    14821 gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc
    14881 ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg gtgggagcta
    14941 aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt gctatatgga
    15001 tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc agtatgacgc
    15061 ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg
    15121 ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc cagggcaatg
    15181 ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct caggagatca
    15241 gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt
    15301 ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag
    15361 gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc acgcagttgc
    15421 tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat ggattaaaaa
    15481 gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac ttttccggtg
    15541 ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg tttgaatctt
    15601 ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc acccggacca
    15661 tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca ctggtggaaa
    15721 ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag atacagcgta
    15781 acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact tcgcagtatc
    15841 tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg atgcgcagga
    15901 tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcgtacaccg
    15961 aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg caggtggatt
    16021 cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg cgcattctgc
    16081 aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg gacggaacgc
    16141 ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc actcatccgc
    16201 gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg ctgtatgtca
    16261 tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag ccgcgcatca
    16321 cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt gatttctgct
    16381 cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg caggaccgac
    16441 cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc
    16501 cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag gtgaactgga
    16561 ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg caggccattg
    16621 tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc cggggacagg
    16681 cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag accgtggact
    16741 tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa atctgcgatg
    16801 atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc cagacccgga
    16861 cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg ataagcctgg
    16921 ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg
    16981 tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg ctgaagctgc
    17041 cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac gacggcacgt
    17101 atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat aacggggcac
    17161 actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg gtgcagcatc
    17221 tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga tgggacacgc
    17281 cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg gacgacggca
    17341 gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc aggcagctgg
    17401 cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag cagggcgatc
    17461 cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt gaactgatac
    17521 cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg acggtgcagt
    17581 ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa accagcgccc
    17641 gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg ccgggccatg
    17701 attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt gtggaggccg
    17761 tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg ttgatcaata
    17821 aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt gcgccggacc
    17881 tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa accgtcaata
    17941 aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag gttgatacaa
    18001 ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac ggacgccttt
    18061 atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
    18121 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
    18181 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    18241 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    18301 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    18361 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    18421 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    18481 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    18541 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    18601 tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac gaccctggat
    18661 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    18721 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    18781 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    18841 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    18901 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgcc
    18961 tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg cctcgacgaa
    19021 cgctcccggc agtgatgatc tga
//
LOCUS       DAJTNW010000083        17164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNW010000083 DAJTNW010000000
VERSION     DAJTNW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..175
                     /locus_tag="OO179_003026"
     CDS             <1..175
                     /locus_tag="OO179_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5533004.1"
                     /translation="KDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIG
                     NLHYIDTGAVFGG"
     gene            complement(200..391)
                     /locus_tag="OO179_003027"
     CDS             complement(200..391)
                     /locus_tag="OO179_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5533005.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(496..732)
                     /locus_tag="OO179_003028"
     CDS             complement(496..732)
                     /locus_tag="OO179_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5533006.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(850..2289)
                     /gene="rsmF"
                     /locus_tag="OO179_003029"
     CDS             complement(850..2289)
                     /gene="rsmF"
                     /locus_tag="OO179_003029"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5533007.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2369..4989)
                     /gene="yebT"
                     /locus_tag="OO179_003030"
                     /pseudo
     CDS             complement(2369..4989)
                     /gene="yebT"
                     /locus_tag="OO179_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4958..6241)
                     /gene="yebS"
                     /locus_tag="OO179_003031"
     CDS             complement(4958..6241)
                     /gene="yebS"
                     /locus_tag="OO179_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5533008.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6371..6868
                     /gene="msrC"
                     /locus_tag="OO179_003032"
     CDS             6371..6868
                     /gene="msrC"
                     /locus_tag="OO179_003032"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5533009.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6965..7663
                     /gene="proQ"
                     /locus_tag="OO179_003033"
     CDS             6965..7663
                     /gene="proQ"
                     /locus_tag="OO179_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5533010.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7683..9731
                     /gene="prc"
                     /locus_tag="OO179_003034"
     CDS             7683..9731
                     /gene="prc"
                     /locus_tag="OO179_003034"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5533011.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9923..10804
                     /gene="htpX"
                     /locus_tag="OO179_003035"
     CDS             9923..10804
                     /gene="htpX"
                     /locus_tag="OO179_003035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5533012.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10850..12223)
                     /locus_tag="OO179_003036"
     CDS             complement(10850..12223)
                     /locus_tag="OO179_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5533013.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12400..13191
                     /gene="kdgR"
                     /locus_tag="OO179_003037"
     CDS             12400..13191
                     /gene="kdgR"
                     /locus_tag="OO179_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5533014.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13334..13573)
                     /locus_tag="OO179_003038"
     CDS             complement(13334..13573)
                     /locus_tag="OO179_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533015.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13732..13875
                     /gene="mgrB"
                     /locus_tag="OO179_003039"
     CDS             13732..13875
                     /gene="mgrB"
                     /locus_tag="OO179_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5533016.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13950..14237
                     /locus_tag="OO179_003040"
     CDS             13950..14237
                     /locus_tag="OO179_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5533017.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14816..14959
                     /locus_tag="OO179_003041"
     CDS             14816..14959
                     /locus_tag="OO179_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5533018.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14972..15181
                     /gene="cspE"
                     /locus_tag="OO179_003042"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14972..15181
                     /gene="cspE"
                     /locus_tag="OO179_003042"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5533019.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15347..16156
                     /gene="rlmA"
                     /locus_tag="OO179_003043"
     CDS             15347..16156
                     /gene="rlmA"
                     /locus_tag="OO179_003043"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5533020.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLRQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16153..16719)
                     /gene="mntP"
                     /locus_tag="OO179_003044"
     CDS             complement(16153..16719)
                     /gene="mntP"
                     /locus_tag="OO179_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5533021.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4527 a         4154 c         4336 g         4147 t
ORIGIN      
        1 aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg aacgcccaaa
       61 agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt tgcgacatcg
      121 cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg gctaactgac
      181 tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc cagaaaccat
      241 ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg ctcatagaat
      301 aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa ccgccatcta
      361 ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt gccgtaccgt
      421 taagtttagg agagatttta caacggggga ataaccagga caaataaccc gccatgccga
      481 cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt gatcgtaacg
      541 ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg tttcagcatc
      601 ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg ttaacgttcg
      661 atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc ctatgcgcac
      721 actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac cagtccgatc
      781 gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt gcgctttttt
      841 tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt cacgcgggta
      901 gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat gctggaaagt
      961 aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc cgcgatacca
     1021 ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg aggcatgggc
     1081 aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa gtttaatccc
     1141 taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga acaaccacag
     1201 ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa cacttgcagc
     1261 cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga aattgcccac
     1321 tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac gagcaacgaa
     1381 gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt cggtcagcgc
     1441 cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt cggggtaagt
     1501 ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac aggttgagta
     1561 aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt cccgttgggt
     1621 agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg gatctttacg
     1681 caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca tttctggcac
     1741 tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga tgccacagcg
     1801 gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg caaggattgc
     1861 cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg cagcagcgac
     1921 atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg gcaacattga
     1981 actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac ccaatggcaa
     2041 tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa ttggcgtaag
     2101 cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt tcagcgtatt
     2161 aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat caaatgagag
     2221 cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat aaacggtgtg
     2281 ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc ataagcgctc
     2341 cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc cattcacgcc
     2401 actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct ttcggtgcaa
     2461 gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg ttaaaggtgc
     2521 cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat gccaaccaga
     2581 agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc gcaatcatca
     2641 cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc agaccacgga
     2701 acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca ataatgctta
     2761 aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag tcgcgacgag
     2821 gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct agatgctcaa
     2881 cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg cgagcaaagg
     2941 tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt gcggtaatca
     3001 gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc atcccgaccg
     3061 ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca cgggccgctg
     3121 tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg gcaccactga
     3181 ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc tgtacggtca
     3241 ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg ctgttgctgg
     3301 tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg ttagctcgcg
     3361 gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc gatcctgcct
     3421 gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa tcgctaaggc
     3481 tgttctccag cgctttttcc agattggcat acagtggata gctggctttc atctcgcctt
     3541 tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg gcaccgagaa
     3601 actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca aatttgctat
     3661 cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg acgcctttgc
     3721 tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg agcggctgac
     3781 ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg tccggctctt
     3841 gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg ccatcgccgg
     3901 gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg ctaagggata
     3961 atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa cggtcatttc
     4021 cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga cttccagccc
     4081 ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca gttccagttt
     4141 tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg tatcttccgc
     4201 ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc
     4261 cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa cgccggaaac
     4321 gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc
     4381 aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat
     4441 cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg cttgcaggtg
     4501 gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg cgacaaagtg
     4561 atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga cgagggcgtc
     4621 cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact gagtctcttc
     4681 gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc
     4741 gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt
     4801 acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat taccccggtc
     4861 ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag gcagcagcca
     4921 gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc
     4981 ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca
     5041 aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat
     5101 cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgtc
     5161 caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa
     5221 aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac
     5281 tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac ataattccgg
     5341 aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata ggcaataggt
     5401 tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat
     5461 gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc tgatcaggat
     5521 agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc
     5581 gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac gtcaccgtcg
     5641 ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata tgggcataat
     5701 cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact
     5761 ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt cggttaccaa
     5821 accataaata tgctatggag gacaccagaa tgaggggggc accaataacg cagaaaaaga
     5881 ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct tgcatgacgt
     5941 tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc caggcaaacg
     6001 gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac cagtcgcgcc
     6061 cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa tttatctcgg
     6121 gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct
     6181 cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca
     6241 tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg aatgatcttg
     6301 agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag
     6361 tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct
     6421 gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga
     6481 gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata cactggtact
     6541 cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg
     6601 cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca
     6661 tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca
     6721 gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag acgaggacga
     6781 gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa
     6841 aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt
     6901 cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa
     6961 tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga
     7021 acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa ttggtatttt
     7081 tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc
     7141 cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac
     7201 gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc
     7261 tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc
     7321 gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg
     7381 caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca
     7441 aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc
     7501 ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa
     7561 cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc
     7621 gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag
     7681 gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga
     7741 ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc
     7801 agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt
     7861 tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt
     7921 acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt
     7981 taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc
     8041 aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag ccgatggatt
     8101 tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg
     8161 ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctga
     8221 caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc
     8281 gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc
     8341 gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg
     8401 aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat gactacaccg
     8461 ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg
     8521 acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt ggctggcgtc
     8581 ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt
     8641 tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa cgtattcgtc
     8701 tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc
     8761 tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac
     8821 tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt
     8881 tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc
     8941 gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag
     9001 gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg
     9061 caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg
     9121 ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag
     9181 cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc
     9241 aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa acggaaacgg
     9301 gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg acttatgtga
     9361 aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat gcgcgtatcg
     9421 cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg
     9481 acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg
     9541 atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa ccggagttga
     9601 agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg gatgagacgg
     9661 tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgctc
     9721 ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga
     9781 tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat
     9841 ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt
     9901 aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc
     9961 cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca
    10021 ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat
    10081 gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga
    10141 aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg ggatcgctat
    10201 gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg
    10261 tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc
    10321 cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg tcaccatgac
    10381 gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc tggcgcagct
    10441 tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct
    10501 gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat
    10561 caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt
    10621 tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga
    10681 agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca gtgagttgtt
    10741 catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg aatacctgaa
    10801 gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt tatgccctgg
    10861 ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc agaatagccg
    10921 cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc agcgccacca
    10981 gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca ctggccccac
    11041 cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac tggaataagc
    11101 caaatccagc gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg
    11161 gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa gcacccagca
    11221 atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc atcgttgcta
    11281 acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca
    11341 ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc
    11401 aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa
    11461 gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag
    11521 caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga
    11581 aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac
    11641 cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtacgttga
    11701 ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg
    11761 cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc agaaaacgtt
    11821 gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca ccgccgaaac
    11881 cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg
    11941 aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc
    12001 ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc
    12061 agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg gcgacgttgg
    12121 cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt
    12181 atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt
    12241 ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat
    12301 tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac
    12361 agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat
    12421 aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt
    12481 gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc
    12541 gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg
    12601 gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc
    12661 gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact
    12721 atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg
    12781 tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt
    12841 ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg
    12901 gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg
    12961 gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga agggctgcaa
    13021 tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt gagcatctcc
    13081 ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc
    13141 gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa
    13201 gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga
    13261 aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt gagaatctgg
    13321 ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac agtgggcaat
    13381 ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg
    13441 cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt ccaatcaaaa
    13501 cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa
    13561 tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg
    13621 cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta ggcgctgttt
    13681 aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg agtgaaaaag
    13741 tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta
    13801 ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag
    13861 tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta
    13921 gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga
    13981 acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc ttgtggtttt
    14041 ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag gcgttgctgg
    14101 atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac gaaccagaga
    14161 aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat attattcgcc
    14221 tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc
    14281 aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa actctggtct
    14341 gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga tttttttgct
    14401 cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac ttctctttag
    14461 ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac tttttattca
    14521 ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc tctggcctgg
    14581 aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc tgcctgaact
    14641 ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa
    14701 gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat
    14761 aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc tgtttatgtg
    14821 tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc gcttctctgc
    14881 cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg
    14941 ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc aggttaagtg
    15001 gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca aagatgtgtt
    15061 cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag gtcagaacgt
    15121 tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa cagctatctg
    15181 atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa
    15241 tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt gcccgccgcg
    15301 tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt cgttttcctg
    15361 tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc cccaacgaca
    15421 tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc ataaacggtc
    15481 tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct tagatgccgg
    15541 acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc ttgatgaaaa
    15601 ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg catttgccga
    15661 tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga taaaagcggc
    15721 ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt tgccgttttc
    15781 cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag aagaattagc
    15841 acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc gacatttgat
    15901 ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg cagaacaact
    15961 ggaaggtttt acattacggc agagtgcgga gttgtgttat ccgatgcgtc ttcgcggtga
    16021 tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc cagaagtctg
    16081 gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata ttcacctctg
    16141 gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga tgccgatcag
    16201 caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa agcgaccaac
    16261 catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg cggtcgtgat
    16321 aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca ggctggtggc
    16381 aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt catcttcatc
    16441 atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca gcagcacaaa
    16501 cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca ttccccagcc
    16561 gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca atgcttcaga
    16621 aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg catccatcga
    16681 cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct gaccggggta
    16741 tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa gccgcgatgg
    16801 gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct gactgaaagt
    16861 cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat ttccggaatc
    16921 tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag aatataaaac
    16981 aactgttgga atatattatt tcattgcttt attgataacg attttcattt atgtttatat
    17041 atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc ctaagctatg
    17101 tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt attgagttga
    17161 tacg
//
LOCUS       DAJTNW010000084        17052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNW010000084 DAJTNW010000000
VERSION     DAJTNW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..213)
                     /locus_tag="OO179_003045"
     CDS             complement(<1..213)
                     /locus_tag="OO179_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5533022.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO179_003046"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO179_003046"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5533023.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO179_003047"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO179_003047"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5533024.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO179_003048"
     CDS             2894..3634
                     /locus_tag="OO179_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5533025.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO179_003049"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO179_003049"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5533026.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO179_003050"
     CDS             complement(4192..4794)
                     /locus_tag="OO179_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5533027.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4863..4928
                     /gene="yobI"
                     /locus_tag="OO179_003051"
     CDS             4863..4928
                     /gene="yobI"
                     /locus_tag="OO179_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5533028.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5067..5678
                     /gene="ruvA"
                     /locus_tag="OO179_003052"
     CDS             5067..5678
                     /gene="ruvA"
                     /locus_tag="OO179_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5533029.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5687..6697
                     /gene="ruvB"
                     /locus_tag="OO179_003053"
     CDS             5687..6697
                     /gene="ruvB"
                     /locus_tag="OO179_003053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5533030.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OO179_003054"
     CDS             complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OO179_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5533031.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OO179_003055"
     CDS             complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OO179_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5533032.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8460..9392
                     /gene="znuA"
                     /locus_tag="OO179_003056"
     CDS             8460..9392
                     /gene="znuA"
                     /locus_tag="OO179_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5533033.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9408..10730
                     /gene="mepM"
                     /locus_tag="OO179_003057"
     CDS             9408..10730
                     /gene="mepM"
                     /locus_tag="OO179_003057"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5533034.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10850..11821
                     /gene="lpxM"
                     /locus_tag="OO179_003058"
                     /gene_synonym="msbB"
     CDS             10850..11821
                     /gene="lpxM"
                     /locus_tag="OO179_003058"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5533035.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OO179_003059"
     CDS             complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OO179_003059"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5533036.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OO179_003060"
                     /gene_synonym="yebK"
     CDS             complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OO179_003060"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5533037.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14728..16203
                     /gene="zwf"
                     /locus_tag="OO179_003061"
     CDS             14728..16203
                     /gene="zwf"
                     /locus_tag="OO179_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5533038.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16438..>17052
                     /locus_tag="OO179_003062"
     CDS             16438..>17052
                     /locus_tag="OO179_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5533039.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4352 a         4204 c         4434 g         4062 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaattatttt tcaggagtcg
     4861 caatgtatat ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc
     4921 caatataacc tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc
     4981 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatt
     5041 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
     5101 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
     5161 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
     5221 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt
     5281 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg
     5341 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
     5401 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
     5461 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
     5521 ctagcccggc gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct
     5581 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac accagcagtg
     5641 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
     5701 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
     5761 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
     5821 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
     5881 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
     5941 gacttctggt ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga
     6001 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     6061 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     6121 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     6181 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     6241 ggtgccggat ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag
     6301 tgatgacggt gcgcaggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     6361 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     6421 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     6481 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     6541 cctggcggca gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt
     6601 gattcagcaa ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg
     6661 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     6721 atccgacact ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag
     6781 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     6841 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     6901 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     6961 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     7021 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     7081 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt
     7141 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     7201 ccagaacaaa atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg
     7261 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     7321 cagactaacg accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     7381 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     7441 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     7501 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     7561 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     7621 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     7681 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     7741 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc
     7801 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     7861 acgccacaat ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca
     7921 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat
     7981 gctcgcgcta atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     8041 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     8101 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     8161 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     8221 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     8281 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     8341 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     8401 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     8461 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     8521 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     8581 ccattgctga tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg
     8641 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     8701 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     8761 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg
     8821 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     8881 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     8941 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     9001 aggcacaatt ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     9061 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     9121 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     9181 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     9241 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     9301 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     9361 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     9421 cccgctctgt cgccttggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     9481 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg
     9541 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     9601 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     9661 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     9721 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caacttgctg
     9781 ctgccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     9841 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     9901 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     9961 acaatctgct gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt
    10021 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
    10081 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
    10141 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa
    10201 tccgcgctga ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
    10261 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta
    10321 atccggtgac aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta
    10381 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
    10441 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
    10501 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
    10561 ataccggacg ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg
    10621 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
    10681 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
    10741 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgtgcc
    10801 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
    10861 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
    10921 gggagcatgg ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
    10981 tgatcccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
    11041 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
    11101 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
    11161 aatacgcggg ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga
    11221 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
    11281 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
    11341 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
    11401 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
    11461 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
    11521 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt
    11581 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
    11641 accgatggat gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga
    11701 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
    11761 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
    11821 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
    11881 attcgaaatc tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc
    11941 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
    12001 atcacgtcgc cttgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
    12061 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
    12121 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
    12181 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
    12241 atggtaatga tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca
    12301 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
    12361 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
    12421 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
    12481 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
    12541 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
    12601 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
    12661 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
    12721 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
    12781 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
    12841 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
    12901 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
    12961 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
    13021 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
    13081 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
    13141 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
    13201 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
    13261 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
    13321 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
    13381 agccttttgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
    13441 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
    13501 ttaggacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
    13561 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
    13621 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
    13681 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
    13741 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc
    13801 cagctgcgcc agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac
    13861 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac
    13921 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga
    13981 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
    14041 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
    14101 tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
    14161 gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
    14221 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
    14281 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
    14341 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
    14401 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
    14461 attacgcctg tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat
    14521 aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc
    14581 cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa
    14641 gctcgtaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac
    14701 cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac
    14761 ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat
    14821 caactggaaa aagtcggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct
    14881 gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa
    14941 gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc
    15001 gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt
    15061 atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt
    15121 ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg
    15181 tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag
    15241 gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt
    15301 tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt
    15361 accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag
    15421 atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg
    15481 ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg
    15541 cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc
    15601 ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc
    15661 aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg
    15721 ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat
    15781 ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa
    15841 ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct
    15901 ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc
    15961 tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt
    16021 attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc
    16081 attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg
    16141 ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag
    16201 taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt
    16261 agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac
    16321 actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct
    16381 ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg
    16441 aatccacaat tgttacgcgt aacaaatcga atcattgaac gttcgcgcga gactcgctct
    16501 gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca
    16561 tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa
    16621 agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac
    16681 cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt
    16741 ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga
    16801 atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc
    16861 cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg
    16921 acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg
    16981 gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa
    17041 gtggaccgca tg
//
LOCUS       DAJTNW010000085        16460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNW010000085 DAJTNW010000000
VERSION     DAJTNW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            109..1110
                     /locus_tag="OO179_003063"
     CDS             109..1110
                     /locus_tag="OO179_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5533040.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1401..1835)
                     /locus_tag="OO179_003064"
     CDS             complement(1401..1835)
                     /locus_tag="OO179_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5533041.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1807..2598)
                     /locus_tag="OO179_003065"
     CDS             complement(1807..2598)
                     /locus_tag="OO179_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5533042.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2598..3278)
                     /locus_tag="OO179_003066"
     CDS             complement(2598..3278)
                     /locus_tag="OO179_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5533043.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3275..4474)
                     /locus_tag="OO179_003067"
     CDS             complement(3275..4474)
                     /locus_tag="OO179_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5533044.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4475..4828)
                     /locus_tag="OO179_003068"
     CDS             complement(4475..4828)
                     /locus_tag="OO179_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533045.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4828..5568)
                     /locus_tag="OO179_003069"
     CDS             complement(4828..5568)
                     /locus_tag="OO179_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5533046.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5791..6081
                     /locus_tag="OO179_003070"
     CDS             5791..6081
                     /locus_tag="OO179_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCR5533047.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6105..6449)
                     /locus_tag="OO179_003071"
     CDS             complement(6105..6449)
                     /locus_tag="OO179_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533048.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6462..7526)
                     /locus_tag="OO179_003072"
     CDS             complement(6462..7526)
                     /locus_tag="OO179_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533049.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7529..7834)
                     /locus_tag="OO179_003073"
     CDS             complement(7529..7834)
                     /locus_tag="OO179_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533050.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7836..8438)
                     /locus_tag="OO179_003074"
     CDS             complement(7836..8438)
                     /locus_tag="OO179_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533051.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8438..10453)
                     /locus_tag="OO179_003075"
     CDS             complement(8438..10453)
                     /locus_tag="OO179_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR5533052.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10631..11056)
                     /locus_tag="OO179_003076"
     CDS             complement(10631..11056)
                     /locus_tag="OO179_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533053.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11060..11500)
                     /locus_tag="OO179_003077"
     CDS             complement(11060..11500)
                     /locus_tag="OO179_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCR5533054.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11511..12656)
                     /locus_tag="OO179_003078"
     CDS             complement(11511..12656)
                     /locus_tag="OO179_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR5533055.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12660..13217)
                     /locus_tag="OO179_003079"
     CDS             complement(12660..13217)
                     /locus_tag="OO179_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533056.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13192..13581)
                     /locus_tag="OO179_003080"
     CDS             complement(13192..13581)
                     /locus_tag="OO179_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCR5533057.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13568..14122)
                     /locus_tag="OO179_003081"
     CDS             complement(13568..14122)
                     /locus_tag="OO179_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533058.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14119..14526)
                     /locus_tag="OO179_003082"
     CDS             complement(14119..14526)
                     /locus_tag="OO179_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR5533059.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14525..14759
                     /locus_tag="OO179_003083"
                     /pseudo
     CDS             14525..14759
                     /locus_tag="OO179_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14756..15697)
                     /locus_tag="OO179_003084"
     CDS             complement(14756..15697)
                     /locus_tag="OO179_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5533060.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15709..16212)
                     /locus_tag="OO179_003085"
     CDS             complement(15709..16212)
                     /locus_tag="OO179_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533061.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16217..>16460)
                     /locus_tag="OO179_003086"
     CDS             complement(16217..>16460)
                     /locus_tag="OO179_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533062.1"
                     /translation="FKREVLASADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKG
                     RNTRSTTGDAQRPNMGMTSIASLNKQNAEFWSNRKG"
BASE COUNT         3856 a         4420 c         4037 g         4147 t
ORIGIN      
        1 tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgaaa tggactatga
       61 cataaagatc tgatattttc ctcacaaaaa cttagggatt ttattttaat gcataagagc
      121 aactgctttg atacggcaag gcttgtggca gctatgatgg ttctaatcag tcaccactac
      181 gcgctctctg gacaactcga gccgtatata ttcggctttg aaagtgctgg cggcattgct
      241 gttatcatat tcttttccat atcaggatac ctgatttcaa aatcagcaat cagaagtgat
      301 tcctttattg atttcatggc taaaagagca agaagaatat tccctgctct tgtcccttgc
      361 tctgtactga cgtatttttt atttgggtgg atgcttaaca acttcagtac agaatatttt
      421 agtcatgaca ttatccggaa gaccataagc tctattttaa tgagtcaggc tccagatgcc
      481 gacattacat cacatttaat tcatcccggt attaacggca gcttgtggac actaccactg
      541 gagtttatgt gttacataat aactggagtg gctgttgctc tactaaagag cggaaaaacg
      601 ttcatagtta tactattgat ttttgtttca ctttcattag ttgaaagtgt atctgagaac
      661 aaggatgtaa tattctccat tccagtatgg ctgtatcctt tgcgggggat ggctttcttt
      721 tttggtgcca ctatggcgat gtatgagaaa tcttggaatg taagtaatgt gaaaatttcc
      781 gtgatttctt tgcttgctat gtacgcatac gcgagctatg gcaaaggcat tgactataca
      841 atggcttgct atattctggt ttctttctca acaatagcta tatgcacatc ttttggagat
      901 ccactggtta agggtaggtt tgattactct tatggtgtat acatttatgc attccccgtt
      961 cagcaggtta ttataaatac attgcatatt ggcttttacc cttcgatgtt gttaagtgcc
     1021 attgtcgttc ttttcttggc tcatctttct tggaatctaa ttgaaaaaag attccttaca
     1081 cgaagctcgc caaaattatc cctgaattga ataataaagg ggccatcgag cccctgaata
     1141 aatcaaagcc agatataaca ctgcctggca gtggacaccg ttctgatgcc tcgtcctgca
     1201 aggacgagcg aaggtggagt tcttttccac gcagctattt agtacagtac tgagtcatat
     1261 tgtttatgta tgagttaact tccgtaatat caattaaata aaataagttg aggccattgt
     1321 agaagtgagc attatatcca ggttatctgg acctgttgaa aaatgcgtat tattccaaca
     1381 attaccctag attaacaagt ctatttattt ggatgcatag gccactcggg ttttactaca
     1441 ttaacacgca taagcagtac gcgatatttt ttccatgcca gtaacagttt cgtttcatca
     1501 tctgttgcca tacctaaatc aaccgcatcc tgaagcggcg ctattacatt cgttgcctct
     1561 gctatcagtg cagatttctt ttcatcattt attaaggcta attgttctgg tgttggagaa
     1621 ggacggtcaa caatgacagg gtgaccgttc tcaccacagg taatcatttt tgttacactc
     1681 tgtgagttaa tcagtgtttt ccactctgat tccgagattt ccactgcatc atcaggaata
     1741 ttggtgccgt gaaattcagt cgaataaaat ccgtttgtcg atgcagaata aaaatatgac
     1801 atttcgttaa tatcccattg caatgtaata cgcatcagca gatgaaccat atcctgaacc
     1861 gggcgacact gtaatatcaa atccagtgtt atggacattt gtcgccgata cagtcggctg
     1921 ccctgacgcg ccagtaacgt tcaatgtaac tgtgacagca aggactcttg agaatgtcat
     1981 cgggaacctc atcccgtatg ttgaagatac attcggggag ccattcaact gaccgcactg
     2041 taaaattaat ccagacggaa atttttgcgc agcagccgtt gcggtattac ttgacgcgaa
     2101 caggctcata tcaggtatct gattcgcccc tgtacctaca ttccttttag ccgcttctcc
     2161 caaaccaagg tatgtgagaa tgtcagcaat agtatttttc ccaataatgt cacggccaac
     2221 agaagttaaa tcagtctgcg ctgctgtatc atttccagtg aaatatggga gtttatttgc
     2281 acctgttgcg agcccagcta atgccgacag cgtggcatca agcgcctgga aatctttccc
     2341 gaaagcggtc cccattttgg atataaaccc gttcaggtct ccatcatcaa gcacatccag
     2401 cctgcttttg ttggcggtgt actgcgccaa cgctgccgcg ataaagctgg cttgccgaat
     2461 agctttgttg acctgtgcgc tggatgcttt acctgccgta aaccctgaaa gcagagccgg
     2521 aagtgcttcc cagtcagctt gtgaggtgac gttagcgttc ggatcaagcg cgaaaggttt
     2581 aaagttgttt attgccatta gagtattgtc ccccatgctc caacatcgaa cccgccgatg
     2641 tattcgttat ccatatcaaa cccaaagaat ttagagccct cggatggtgc ttctaccgaa
     2701 ggcgtttcaa catcaccggc ccatacgcca gctgctttaa cggtgagata gccctgtttg
     2761 atagcggcaa tcagttcaag agacacatca gaaatatcag tctcggggaa tacccaaacc
     2821 gatatcgtca tgtcctggtt gtcgacaatt tgcatcctca ggcctgagcc ttcagtcgca
     2881 gcgtcaagga tgggaggcag agagtcgttc cgaccgtccc agttgttgat agcgattttc
     2941 gctttcagaa tgatgcggta cgtctcatcg cttagcgtcg tatagccaga atcaggatca
     3001 tatggccctt tccagatgcc ctggtcatac ccaagcccgt cagtgtccca gctgaaataa
     3061 actccgctaa ttggctggct gactatgcga ctgcgtccga tccacagacc gaggatgtcg
     3121 agctgtacac cgacagcagt atcaatatcg aaggctgtta taagccctga catagtgctg
     3181 gacacatcaa tcagcgggcg ggtgctcaga tctatatggt caaaaaagag tggcttggta
     3241 gcgtggtagt tagtgatcag ttcggtgtat ttgctcatga ggtcaccgtg atactgatat
     3301 tcgcggtgct acaggacgca gaagcatcat aggcaatatc aatgtttgat gccgatacgc
     3361 tgccagacga cttaccgatc agcaggtcgg taatatcgta atagcgggca ttcccgccgc
     3421 tcacaacgcc gaggtttgcc ggggaataaa tacggctcag cagaacgtcg tcgccaattg
     3481 ttaggccatt tatataatcg gcaacagcct gtttaatctg ctcgccgatt tgagaggtat
     3541 agccggtaaa aactttcagg gtaatggcta cgaaaattgg cacatcggta gagcgcgaaa
     3601 aactgatgac gtgtggatta ccgtaagtat ccggcaccgt gacagaagtt ttaccgtaag
     3661 ttgcggttcc ctgcccttta ctcccccgga ttgtttgggc tatctcggta acatcccctc
     3721 catcgacgat ggcggaaata gagtgtggcg gcagcccgtt gctgtcggtt gccccagtgt
     3781 cgttctcata tagtttgtga cgtgtcacgc cagcaacatt agcgatagca ccgtcgacac
     3841 cttcaaacgg tgtgatcgat ggtagcgcga cgctttgccc ctgccgaatg cgcagctctg
     3901 cgtcggtttc ggctggtgaa ccgacagtag ccgcaactgg attggttacc gacacccaac
     3961 ctcgggtcgg ggtgttgata gtggtaatag tcccggccag cgccgcaacc gaaccgctat
     4021 tcgcacatgt ggccgtcacc agcacagtac catcaacgcc gatcgctaca cttgcgggaa
     4081 aattccagat aatgccgttt ttatcccgtg cggagccatt cgtgatagtc gtgcctgccg
     4141 taccggttaa cagaaggtca gcagtagagt ttgtcgctac ttttcgcgtg atcccgttaa
     4201 ttttcacatt gctgctaagc gctgcggcct gcgctgtcgt cggtgaaaaa gagttgtaga
     4261 tcccgatagc ggtgttgtta gcgtcatgca cggcaagagc caccagcgcg accatctgcc
     4321 cgtctttgct gtctggttcg aggtaggcat cactaccgta aatctgcctg aaatagctgg
     4381 tcagtgtatc taggattgtc tggtaatcag gcgcactaat cccctgggcg gttaccgttg
     4441 ccgatagccc cagcgtgtcg aggttcaaag ccatttatgc ctcgcttgtt acagtcgtct
     4501 ggccgtagat tgtgtcaatg gaggaagtga aggtgacgcg acggctggtg ccgtcataat
     4561 tggtatcgaa ggaaagaatc gacagaacgc ccggtgtatc ctgtatgcgt tcgcgtatag
     4621 ccaggatgta gacatctgat cgctgcttcc caagcactga ctgaacatac ggcgtgcctt
     4681 ccgtcagatc gagaaaccac tgaccgcgcc acagctcgaa acgggttttt acggcctggg
     4741 cgacacattc cggactgtcg ataaggaaag tatcgtcacc ctggccgaaa gtgtaatcgc
     4801 cttcagtatc ttcgcgacgg tatcgcatta ttgcggccct ccggtagttc ccccgccagt
     4861 ctgtactccg ccatgtttat gcgtggcgac actgatacca gaagctgtta cgtcattcgt
     4921 taccatcacc ggcccaagca tcgtcgcagt accgccgctt tcgcccattc cctgagacaa
     4981 gttgccatta atcgttacgt tgccgttcag cgtgatagtc ggggatgtga ttgtcgttcc
     5041 accttcagcc gtagctgtaa gctggcccgg tgttttaatg gtgatgttat gtcctgcggc
     5101 gacttctaca aacgccgcac catcatcggt tcgcagctgc gcggcgctgg tactgatacc
     5161 gctgattttc cgtgcttgcg actgcgggcc aacgatggcg aacgcatcag ataagtcatg
     5221 ctggcgcggg tcgacggtct cctgaacgcc gccgctctgc caccaaaaat cgatgcaacg
     5281 gtcagcaaaa atcaggaggc actcgtcgcc ttcttttacc ggaaaggtca gcgtgcaacc
     5341 gccgccgcgc gggaagatga ccggcacatc caccagcggt tttaattcgg tggatccatc
     5401 gccaacaata ccgcgtagcg ccacctctac tgtgcaggta acagtctcag gatcgaacga
     5461 ctgaatgatg ccgggcatcg ctacgcgcat ctgggtagac accgaatcgg caatggcctg
     5521 cgcggtctgc tgctcaccgc cgatctgtga ttgagttgga attggcataa aacccccata
     5581 aaaaaaccag ccgaagctgg tttggtatca attatctctt agtaactaag ctacatcagt
     5641 gtgaagtaag gcttttccgc tgcaatggca cacattcaca aggtgtatta ggatatgttt
     5701 gcaacaagta agcattgaaa atcaatttca gccatgaagc cgccatagag tggacctgca
     5761 ccatcgcatt attgactaca ggagtaacaa atgggattta gatttcgtaa aagaatccgg
     5821 attgcgcccg gactcgcgat caacattagt aaaagcggag taagcacttc aattggtggt
     5881 aaaggttcca ccattaacat cgggaaaaaa ggcgtaaaga tgacaaatgg ccttcccggc
     5941 acggggctgt cgcataccac taacctttac tctcccggga aatcgacaga aaaaaaacaa
     6001 cttactcata agcaaaaaat aattagaaac atcctgttcg taattattgt gtttataata
     6061 attaaagctc aatatttttg acgcatgccc gcctatctgg cgggctattt tacttttcgg
     6121 cagtcgtatg ttgcatactg acgcggcgaa tccatgctgg cttgcagcca ctgtgcgttg
     6181 agaatggctt tgccgtttcg attgatgtac tcaagaccaa cccatcttcc aggctgatcg
     6241 gtagccatac gccaatccat cttgatattg ttataatcgc ctttgttttt caggaaggtg
     6301 attttttgca tttctggctt tgcgccattg attctggcta agccatcatc tgcccaatgg
     6361 attttaaaat caccacattg ctgatccgca aaagcgggtg ctgatataag caccgcgaac
     6421 acggtaacag tgcaaagtaa atgtctcacg gccccacctc actaattcgt cgtcccttgc
     6481 attgctttcg ggctataaag atcacgagcg ccacgcgcaa aacacatcaa atccatgtac
     6541 cacgcctgac ctctggtgtc gccagtatag tcgatagctt tgacgatata aacgccatcc
     6601 gtcgcaatgc tggcagcctg tgacgtcgtg ccggtcagca cacggttgcc gttctcttct
     6661 gtttcggtga tacgcccggg cgactgtgct atttcgctat tgccgagcgt ggcgcggtac
     6721 accgaagcct gatcgagctg gataagacca ttaatacgga tgtttgggtt tatcaggcat
     6781 cgcacgttta ctccgccacc catcgtttgt tgcggcatac cgatcaggcc agtattggca
     6841 ttcaacacaa tggcttcgtg aatatattta tcctccggca ccatctggac ctgaccatcc
     6901 accagttgcc atgtcgcttt gcactgcgca gcaatattat ccatcacgtt gcggctggat
     6961 gagtaaatcg cgcggccacg aggaaacacg gtatcaggaa aatcgccggt aatgccctgt
     7021 gtcacgccga acgcgttgaa atcctgcatc gtcgcccggt gcagatccgc aacggtatat
     7081 ccagcggcaa gcgtggtgat ggtggtcgca tagaggaacg cttcgtgatc accaatggcc
     7141 tgaatcaaca cccaggaatc cgtaatgttg tccttcccgg tgacggtgaa gcgaatatca
     7201 ccgtcaaaaa tcaggccgta gttctgaccg ttcacctgcc ctatctggtc tggtgaaatc
     7261 tcccgggaca caccaacctg gctcgcatca acatccggcg ctataccgtc atacccggca
     7321 atgatgcgaa ttttggcaaa ctcctgccca agtatcttgt tcgtggtatc ggtcgaaagg
     7381 ttgtaaattt tcacgttcgc cacgcgcggc cagcgtgtgt ctgcccactc gatctggaac
     7441 gtgaccttaa agtcagacag ggaaacgccc tgcccgttct ggtccaacag ttgcagctca
     7501 aaatggcgca tccagttaag agacatttct actcctgtac gaaaatgagg tggctgtatg
     7561 tgccgaggtt ggttttggtg ggctcgtctg gtgcgccctt atcggtcgcc accaccagcg
     7621 cgccatcaat gccaagctgt ggatattgtc gtaaaaggtt tacgccggtc agtagaggta
     7681 cgccagagag aagcgcggca ccgccgctat ccatcacgtc cataatccag ccagccgcat
     7741 cacgccagat gatcctgagt gtatacgtgg tattgcccag caaaactcgg aactgctgat
     7801 tgtcaggaga aagcggtatt tcgttaaact gcatatcatc ccccgaatgc tgatgtaacg
     7861 cttccgccca gctggctcag caaggattcg tttggcggtg tagtggattt catcccggaa
     7921 ttctgcaccg ccgatgtgct gacgccatcc tgcatatctg atttatctgc aacgctaacg
     7981 ctctgcgtct gcgacatgat cacttcacgc agggtaagcg tgcagttcag cacgttctcg
     8041 ctggttttat ccgttgtcac ctcgatggct cgcaccagca tattgctgta aacccgcttt
     8101 ccggtcacta catcgaacgg caccctagag gactggagat ccagtagttg ctgataggtc
     8161 tctttcgggc taagtccggc gctgaggccg attgacgatg tatcaatgaa gtccagcaac
     8221 gaaccgccac ctgcgaagcc gcattccatc gtgacttcgc tggggcgctt atacgcatga
     8281 tcggcgatga aacccgacgc gctattcgtt gttggcttct ccaccgggtg ctcagtaatt
     8341 tcgagcgcat cagaatgctt ttcagaaaca accacgctgg ggattaatat tccaattcgc
     8401 cgggattgct ggcgaaaaat tgctgataaa atatccatta tctcggtcct gcggggagtt
     8461 gctgggttaa ctgtgaattc acgccctttt gacggtcaac agtcaaacgg gcagcctcgc
     8521 gcggatcgaa aacgccgtgg atgttaatat tcgtttcctg ctgaatcacc ggggcgctgg
     8581 tgggcatatt gctcattact ttcggaatgt agttgcgcgt ttcctgcggc attagcccca
     8641 ttccataacg cttaacattc ccgatccccc agttatatga tgccagtgct ttgctaaggt
     8701 ctccgccgtt ctgccgcaac agctggctca ggtacttagc ggctgcctga gctgactttt
     8761 ccgggtcgaa tacatcattc ccacgcaggc ccatgtcgcg cgccgtgcca tccataaact
     8821 gaaacagacc tttagcgcca gcgcctgaaa cggcgaactg attaccgccc gattccgtta
     8881 tggccacgct gcgcaataac ccctccggaa gccggtagag ctgttccagg ttggtaaaca
     8941 tcggctgcat ccatcccagc agttcagagc ctgctttggt tggctgtggc cgcttaactg
     9001 actggccgag ctgctcaggc tcatcatcac cgaaccagcc gcgcaccgtt cggcccacgc
     9061 tgcgaggatc gaatccccag tgctctttaa tccagtcggc ggttccgttg gcgctgtctg
     9121 ttaccatcgg catcgctgac ggattttcgc tgccctgatt aagcatctgt ttgccgatgc
     9181 tggcggcatc agcccagcgg ccatctttaa tggcgttgag caggtcggcg atcatgttca
     9241 gcatcttgcc gaactcaccc atttgcgaaa tgaagttgct aaaatcccac tttagggacc
     9301 atgacttggg gtcaataccg agcaacttag ccagcgcttt tgtcaggtcc gtaacagtct
     9361 ttttcaggtc accaatcatt ttgacggcct gatcaatttc agtcttccat ttcccccagt
     9421 caatcaggct tttgccacct tctttccacg tctggtaatc atccaagagc agaccaagtg
     9481 caataataag cgtcgttatc atgcccaagg gtgatgacat gaaagcggta tttagcagcc
     9541 gccatgcaac caacagccca ctgaacaatg cgataagctg ctgcgtggct gggttcagct
     9601 ttttgaacca ggcgatcaca ccctcaacag cttgcccagt tcgccataac acgcgggtaa
     9661 tcgcgtcacc tgcccagaga attcctttga tgatcttcgt gatgaccgct tcaatcttcg
     9721 gccagttgtc gagtatctgc ctgcggaagt tatcaatact gccagcaagg ccaccagcta
     9781 ggttagagcc aatcttgtct ttcgcctgtc caagcgtcat cgtcagatta cgcatggagg
     9841 tcatgaaaat attggactgt ttagccgctg actcagcatt aaaaccaata cgctttgccg
     9901 tcaacgcgta ctcagaactg agctgcccca tccctctgcg catcgccatc agcgtgtttt
     9961 catcgatgcc gagcatctgc gcgtattgct tcgcgcggta atacggcatg ttgttgagct
    10021 tttggccaac gccagtaaag atggcagcag tatcacgcat ctttccgctg gcatcgcggg
    10081 tctggacacc aagacggttc aggaaccctt ccgcccccgg attgctacgc atgaagccag
    10141 ccaacccttc gagggaggac atagccgact cggcgctggc accggtttgc gatgcggcgt
    10201 atcccagcgc tttgatgccc tggacactgg cccccgtccg ctgggatgcc cagtaaattt
    10261 tatccagacc gttcgcgatc tgagtggtaa atccgacaat gctcagcgct gcgcctttca
    10321 ccaccgcgcc gaccttcaga acgttcgcgg taacgccttt cagcacggct tcaaacttat
    10381 tagcgccagc ctgatcgata tcgaatccca gcgaaacaag gaaatcttta atcgtatctg
    10441 cgttaccgct cattggccgc tctccattta tctacccggg cgtcgttatc ctcgcgcatg
    10501 tcgaggtagt cattgagaag cgcgatgcgg caaagatcta ccgcaccgct gttaaggtct
    10561 ttctggtcaa tatggaaggc aagcgccggg cgaagaataa aatcttcacc gcccggcagg
    10621 ctgttgaagg ttattccgct agcggggtgg gcgtctcgct ggtagggagt ccttgcaaaa
    10681 aatttcccag cgagtcggcg accacccgcg ccaccagttg cagcatggta agcaggtcga
    10741 tatcgtcaaa cgccatttcg ccatgctggc agaccggcac ccagcctttc atgtgctcgc
    10801 gtgaaacaac ggaaagacag gggaacagga tagcgtccac gtcgccatca ctcagatcgg
    10861 acacagcatt ggcaatcttt ggcaggatag tagccatcgc gccttcggtg tctttgctgc
    10921 tgatcttctc ctgaacgctc cggaagtccg aaaccatccc ggccagaacc ggcaacagct
    10981 tgcgggacac cttcagctgt tcgaaaacgc tgagctttgc ggtgcgatat ttcacgcctt
    11041 taatttcgaa ttccatgcgt taaaactccc cgagaagctg gtcaatcttg ccgcagtcga
    11101 atacccaggc gacggttccg ccctctttag cgttattgaa atcaggctgt ttctggaatg
    11161 cacacgaacg cgcagtagaa atatcacccg atgccgtgtt gcgaatgacg atcacgttat
    11221 tgccccaggt ggcagaggac tggctttgcg cgttatacgc cagagacagc tttttattca
    11281 ccggggaggt tttgagtagc gtcaccgtaa tggtgcctga cttatcggcg tgcaggctgt
    11341 gcatcacctc gccatcggca ccgatagtca tggtgttttt gttgccgccc atggtctggg
    11401 tgataccttc ctcagagttc gcagaaccct gaccaagatc gataacgccg gtcggcccgg
    11461 tgagcgacgc ggttacatcg agaaaagaat aagttgccat ttatcgctcc ttagcgaacc
    11521 acgttgatct gcacatcggc ataatgaact gcgccagcca gcttacaggc cacctggatt
    11581 aacggtgctt tgcgcgcttc acggtcggcc tgcgcctgtt cggacagcgg ttgcgcatac
    11641 acgtaatagc ctttggtcag cgtatcgccg gaattcagct gtccgatagg gccaccattc
    11701 cacacgccag cagctaccag accgttcgtg acggactgat ccatggactg ttcaacgttg
    11761 gaaagcagac gggtcacacc ggcatcagtc tgcggaattt tggtggtgct ggtgtaaagc
    11821 aggttataga ggttggtctg aacgtagttc tgcaaccagt cgagcccgtg gcgctcgtcg
    11881 aagaagtcac cgttcgccat gacaccctgt tgcaggatcg ccgtgtcgtt ggcgtagtac
    11941 acgaatacat tgcagttttt tgcatcaagt gccgatgcct ggctgactgt cagtgtttca
    12001 tacccgacac ccggctcctg cttaaacttg agcgtaatcg cggtattact gccattgaaa
    12061 ttaaccgtga atgcccggcc aaatgcagat aacgcagcgt atttattacc cgatgaatat
    12121 tgaataaaac tgcgtgaata tccggcggtt ttcagttttg atgccaaatc atcgctggat
    12181 gcagtctgca ggcatttctc atcgcttgtc gtaatcgcca gaatacggct tacagaagag
    12241 gattcgatcg ccgcagccac tttcagccag tctgcatccg gaatatcttc atcgtctgca
    12301 atccccagcc cataccatga agtataatcg agcatggcat tcacagcctg ctccagcgtc
    12361 tcaggcgtgg cctgttcgct gtctcccttc gttttcaccc aacgaccaac aaaaacctcc
    12421 tgaggtttcg gtgattgtga gaaaaacacc tgcgcagcct tatattctgg tgattccacg
    12481 ccaaaatctt ttccaatatc ttccgcggca gaataacggc gaatgcgctc acttaccgga
    12541 atgattgtgg acgggccgag aatgagtaat gcaccaaaat ttcgccctga tgctgcacgc
    12601 ggcgacatga tcacatcaac attaacaacg tttgatacag gcaagccctg tgccatagct
    12661 taatctccga aaaagatgac tggtgcttcc accagcgatt taataccgta ctcgcgcaca
    12721 accttccggc gcagacgcac cgtcatatcg tagcggcgga cccattgctg attaataagt
    12781 tcagggaaga gacctgtgta atcgccaaga gacagcccca gcgcattcag tgctgcgttg
    12841 ttctgcggta cagatatacc gtcacgaaac cgggacgcat acaccatccc cgccggacca
    12901 taaaacgaag ccatacactc aatcgtttca tgccgccaga gctgagagcc atcatcggtc
    12961 tgtctggtga atgccggact gtcatcacct gaccatccga taaccccaaa cgcacaccag
    13021 ttcgtttcaa ccggtagcag tggcggctgc tctttctgcc agcgcgggcg aaccatcccg
    13081 gcagacagac cggaaacgtt acgcatccac tggcttaaca gcctgtcgag cgcttcgtca
    13141 taatccggat cgccactggt tggtattaac catccgcgct ctgtactggt gttattgctc
    13201 aaccggagtt cccccatcaa acggcatcaa ctcacaatgc gcctgaacga atccggcccc
    13261 ataagctgta tacgggtcga cgaaggtcac acgataatca cggccctgat acgtcacgat
    13321 atcggcatca cggccagtct gtccctgcgt cagtcgctca gtcgtcacaa tcagaattgc
    13381 accgctgatt acctgccctg cctgcatacg gcggttttcc agagagcgat caacagttac
    13441 gactccggca aactgctttt taacttcact gtcgctgccg atcccgtcct catccaccgt
    13501 ttgcactcgg cgtgttaccc acaaattgaa gtcgcaaaaa tcggggtcaa aaagcacatc
    13561 tgttacatca agagtcggca tctttatccc tcacaacatg ggtaatagct ctgcgatatt
    13621 gcccggtgtc aattaatggt ttcgccagat cggttccggg agattcgcca gcaacacgcc
    13681 gagcaagttc cagtgttgcc cccttgcgcc cccgacgagc ccgggcttca acggtgctat
    13741 cagcaagcgg tgtaaagccg gtaatggtca tgtaacgcct gacgccattc gcggccagcg
    13801 ttccggcgcg gttaagcgct ctttccgcac ccgccgcatt tccatcaagc gcagcctgcg
    13861 ccgctgcttt gagctgcggc accgtctgtt cctctacgga tttaacgccg gggatcaggt
    13921 gcgggcgtgg gggtatgttt tgcgctggtg agccgtattc gttgacgtaa ccgatcccgg
    13981 cattaccaaa cggaacatcc tcacgctcgc tgtcttcttc cgggatgccc accagcacat
    14041 cctttttggt tagcgaccgg agcgcatcca gaatggcctg agcgttatca accctcgttg
    14101 ttacaccact tttgaaactc atagctggcg accgcccgca ccgaacatcg tgatcagctg
    14161 ataaaattca gcgccatatc gggtgttatt ccagaagcct gcgtcagggt ttagcgtcgc
    14221 gctggtgtca tagctgacgc ttaccttgtc aacggacttg gaggactgaa caccattggt
    14281 tgaaccgccc gggccgccaa ccagcattgc ccgactatct gccgcccata gcgtcatgta
    14341 gtgagccacg aacaacccgg caaagtacgg aaacaaatct ttgccggtga cgttttcgct
    14401 cagcagttca tcggccagat tcagacgaaa ctggatttgc gcttcgggat atttggcagg
    14461 gtcagcaaac tgcgggaagt cgcggcgaaa atcacttacc gctggcaggc tttgattctt
    14521 tggcattttt tacctcgtta cgcgcgtctg tggctttgcc aacggatact tccgcgtgcg
    14581 cacgagtgaa ccagtgcgtg gcaacgtctt cctccactgc atgacggcct ttaacaaact
    14641 cgcgccgcga accgtcggga agcgtgagca caaacggggt atgtacgtgt attactgcat
    14701 tattttttgc catcgggtca tccttaatgg ccccgccagg ggggccatgt ggctgttaaa
    14761 tgccatcaac gtacgaaatg gtttctttgt acactggctc aaccgcaccc agcttgccgt
    14821 agtaggtcgc aatctgatac agaccgcgat actggatagg aacgctctgc aacggcacca
    14881 gcggatagcg gacgtatttt ttatcgttgg tgtacgcaac catgcgatcc ttattcccca
    14941 caccacggcc tttcagccat ttaaccgcgc ggatattcag cggaacaccg ttctggtgat
    15001 agctgatggt gttggtctga aggtacgtca acagggactg gttacccgca gatgaaacga
    15061 tgatgctgga caacagagca aactgctctg gcgggatcag caaatcacgc ggaaccacag
    15121 agtaaccgga agcggcccac gcatcagaca gcacctggtt aatgcttgcg cggatttcgt
    15181 ccggtgttga gattgcccac gttttggcag cgttgttgac aggcacgccg tccagggtaa
    15241 caaggccttt caggtttaat gcggaatcgc caacatacac ctgctcgtcg ttgtccatct
    15301 gccatttgag ctgcatcccg tcgtacttct gggtgtcaat cgggcgacct acctgctgag
    15361 ctgctgccag ctctacaacg gtccagccca gttccatgcc ccagagattc agcgggttac
    15421 cggatttgcc gatatccacg tttacgccag caatagcggt tgagtctttg cctacccagt
    15481 ttttgccatt cggatttgca ccagtacccg cagcggcgaa gctggtattc gtccagctgg
    15541 aaatgtcatc tgcgatagag acatcttcac gcaactgaat atcgcgggtc caggtgtacc
    15601 ccgccagtgg caggttcaga gtctggtcga gtcgctccag ctctccgatg agaaaggcac
    15661 cagagctgtc aacggttgcc tgatcaaaag taatcattcg tctgttcctt aaatcttcca
    15721 ggaaatttct gcattgccgt tagcatcacc ggcacctgtg aattcagcgt tggtcagcac
    15781 cacatttttg ccactgactg acgtggacat gaatccaccc agcggcactt tgatggattc
    15841 atcagtggag acgacaacgt ataccgggtc gccttttttg atggtgctgg catcaaaatc
    15901 agaaccgaga ttaacggtca cgtagccacg cttcatggcg tcgcccggga agttcttgcc
    15961 tgttcccacc tggcgaacca tgtccggctg cgaagtggtc ggataagggc gcacgtagat
    16021 ccccttcacc ttgtctgcgg tatcgccatc tgccagcggc acgaaaaaac cgtcagcatc
    16081 gtatttacca gccagcccat aggcagcaaa ggcgttatcg gatttaagga ccaccggttc
    16141 gacggttaag tcctgcgggc gagagatagc cccggcaatg ccaacaggca tccggtacag
    16201 atatgcagtc attggattat cctttgcggt tagaccagaa ttcagcgttt tgtttgttca
    16261 gggaagcgat gctggtcatg cccatgtttg gacgttgtgc atcgcccgtg gtgctgcggg
    16321 tgtttcgccc tttggcaatc tcagacacag cgttaaacgc catatcgacc gattgcttag
    16381 gcaatttgcg gatatccgca tcaccgacaa cctggcgaac cagcgttttg tcagcagaag
    16441 ccagaacctc gcgtttgaac
//
LOCUS       DAJTNW010000086        16386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNW010000086 DAJTNW010000000
VERSION     DAJTNW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO179_003087"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO179_003087"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5533063.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO179_003088"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO179_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5533064.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO179_003089"
     CDS             2337..2552
                     /locus_tag="OO179_003089"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533065.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO179_003090"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO179_003090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5533066.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO179_003091"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO179_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5533067.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO179_003092"
     CDS             5918..6415
                     /locus_tag="OO179_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5533068.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO179_003093"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO179_003093"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5533069.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO179_003094"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO179_003094"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5533070.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO179_003095"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO179_003095"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5533071.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO179_003096"
     CDS             9100..9663
                     /locus_tag="OO179_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5533072.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO179_003097"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO179_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5533073.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO179_003098"
     CDS             complement(10255..11235)
                     /locus_tag="OO179_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5533074.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO179_003099"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO179_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5533075.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO179_003100"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO179_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5533076.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO179_003101"
     CDS             12538..12828
                     /locus_tag="OO179_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5533077.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO179_003102"
     CDS             12836..13429
                     /locus_tag="OO179_003102"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5533078.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO179_003103"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO179_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5533079.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="OO179_003104"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="OO179_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="OO179_003105"
     CDS             15880..16383
                     /locus_tag="OO179_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5533080.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4057 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgg
//
LOCUS       DAJTNW010000087        15923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNW010000087 DAJTNW010000000
VERSION     DAJTNW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO179_003106"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO179_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5533081.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO179_003107"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO179_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5533082.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO179_003108"
     CDS             complement(941..1408)
                     /locus_tag="OO179_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5533083.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO179_003109"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO179_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5533084.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="OO179_003110"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="OO179_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5533085.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="OO179_003111"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="OO179_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5533086.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="OO179_003112"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="OO179_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5533087.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="OO179_003113"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="OO179_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5533088.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="OO179_003114"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="OO179_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5533089.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="OO179_003115"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="OO179_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5533090.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="OO179_003116"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="OO179_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5533091.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="OO179_003117"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="OO179_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5533092.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="OO179_003118"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="OO179_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5533093.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO179_003119"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO179_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5533094.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="OO179_003120"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="OO179_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5533095.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="OO179_003121"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="OO179_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5533096.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="OO179_003122"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="OO179_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5533097.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="OO179_003123"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="OO179_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5533098.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="OO179_003124"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="OO179_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5533099.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="OO179_003125"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="OO179_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5533100.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="OO179_003126"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="OO179_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5533101.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="OO179_003127"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="OO179_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5533102.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="OO179_003128"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="OO179_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5533103.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="OO179_003129"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="OO179_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5533104.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="OO179_003130"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="OO179_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5533105.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO179_003131"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO179_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5533106.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="OO179_003132"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="OO179_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5533107.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="OO179_003133"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="OO179_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5533108.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="OO179_003134"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="OO179_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5533109.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="OO179_003135"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="OO179_003135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5533110.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15060..15443
                     /gene="rplQ"
                     /locus_tag="OO179_003136"
     CDS             15060..15443
                     /gene="rplQ"
                     /locus_tag="OO179_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5533111.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15613..>15923
                     /locus_tag="OO179_003137"
     CDS             15613..>15923
                     /locus_tag="OO179_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5533112.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT         4116 a         3749 c         4211 g         3847 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt actattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccaatcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcga
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15061 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15121 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15181 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg
    15241 ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta
    15301 acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg
    15361 gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga
    15421 aagcagaagc tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt
    15481 ttttataccc gtagtatccc cacttatcta caatagctgt actctttttg gtaagcgccc
    15541 catcagcgac gtcttgtgaa aattgtcctg tctggcaaca atcgcgccca tctatattga
    15601 tggacacgaa cgatgaattc ccagacaaca aaagatattc cctgcttccg ttcttatttg
    15661 cctgatgccc tgcgtttaag atttgaagat aaactgacca tccgggccat cgctcagcgt
    15721 ctgggtctca gtcattccac aatacatacg ctttttcagc gatttcttgc atccggtatc
    15781 gcatggccat tgcccgattc agtttcattc gctcaacttg acgccatcct ttatgccaac
    15841 agaaagaagg aattaacaga gcctcaaatc agagaaggct catggcgaaa agaacggcga
    15901 accagctata gccgtgaatt taa
//
LOCUS       DAJTNW010000088        15410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNW010000088 DAJTNW010000000
VERSION     DAJTNW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..1175)
                     /locus_tag="OO179_003138"
     CDS             complement(<1..1175)
                     /locus_tag="OO179_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5533113.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO179_003139"
     tRNA            complement(1374..1458)
                     /locus_tag="OO179_003139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO179_003140"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO179_003140"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5533114.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO179_003141"
     CDS             2802..4304
                     /locus_tag="OO179_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5533115.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO179_003142"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO179_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5533116.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO179_003143"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO179_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5533117.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO179_003144"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO179_003144"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5533118.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO179_003145"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO179_003145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5533119.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO179_003146"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO179_003146"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5533120.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO179_003147"
     CDS             complement(11869..13065)
                     /locus_tag="OO179_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5533121.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMICKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO179_003148"
     CDS             13258..13761
                     /locus_tag="OO179_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5533122.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO179_003149"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO179_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5533123.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OO179_003150"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OO179_003150"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5533124.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3977 a         3932 c         3966 g         3535 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacata tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTNW010000089        15345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNW010000089 DAJTNW010000000
VERSION     DAJTNW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO179_003151"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO179_003151"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5533125.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO179_003152"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO179_003152"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5533126.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO179_003153"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO179_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5533127.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO179_003154"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO179_003154"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5533128.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO179_003155"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO179_003155"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5533129.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO179_003156"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO179_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5533130.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO179_003157"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO179_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5533131.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO179_003158"
     CDS             complement(8065..9207)
                     /locus_tag="OO179_003158"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5533132.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO179_003159"
     CDS             complement(9216..10229)
                     /locus_tag="OO179_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5533133.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO179_003160"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO179_003160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5533134.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO179_003161"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO179_003161"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5533135.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO179_003162"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO179_003162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5533136.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO179_003163"
     CDS             complement(12827..13951)
                     /locus_tag="OO179_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5533137.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO179_003164"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO179_003164"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5533138.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3446 c         3146 g         4374 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg gaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgctaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcagc aagaattggc gggtaaattg ctatttaagt caaaa
//
LOCUS       DAJTNW010000090        15100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNW010000090 DAJTNW010000000
VERSION     DAJTNW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OO179_003165"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OO179_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5533139.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO179_003166"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO179_003166"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5533140.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OO179_003167"
     CDS             1533..2480
                     /locus_tag="OO179_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5533141.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OO179_003168"
     CDS             2550..3356
                     /locus_tag="OO179_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5533142.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OO179_003169"
                     /pseudo
     CDS             3366..3773
                     /locus_tag="OO179_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3773..4267
                     /locus_tag="OO179_003170"
     CDS             3773..4267
                     /locus_tag="OO179_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5533143.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OO179_003171"
     CDS             4333..5130
                     /locus_tag="OO179_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5533144.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OO179_003172"
     CDS             5141..5929
                     /locus_tag="OO179_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5533145.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OO179_003173"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OO179_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5533146.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OO179_003174"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OO179_003174"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5533147.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVLLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OO179_003175"
     CDS             complement(8372..10003)
                     /locus_tag="OO179_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5533148.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO179_003176"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO179_003176"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5533149.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OO179_003177"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OO179_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5533150.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3937 g         3590 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatag cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaga agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtagaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg catggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggcgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctagtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttctgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgtacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca ggttctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt aagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgctcaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tgtcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 caggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa
//
LOCUS       DAJTNW010000091        14947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNW010000091 DAJTNW010000000
VERSION     DAJTNW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(489..1430)
                     /gene="fabY"
                     /locus_tag="OO179_003178"
                     /gene_synonym="yiiD"
     CDS             complement(489..1430)
                     /gene="fabY"
                     /locus_tag="OO179_003178"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5533151.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1475..1912)
                     /gene="dtd"
                     /locus_tag="OO179_003179"
     CDS             complement(1475..1912)
                     /gene="dtd"
                     /locus_tag="OO179_003179"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5533152.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1909..2781)
                     /locus_tag="OO179_003180"
     CDS             complement(1909..2781)
                     /locus_tag="OO179_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5533153.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2775..3374)
                     /gene="yihX"
                     /locus_tag="OO179_003181"
     CDS             complement(2775..3374)
                     /gene="yihX"
                     /locus_tag="OO179_003181"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5533154.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3473..4258)
                     /locus_tag="OO179_003182"
     CDS             complement(3473..4258)
                     /locus_tag="OO179_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5533155.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4292..5188)
                     /gene="yihV"
                     /locus_tag="OO179_003183"
     CDS             complement(4292..5188)
                     /gene="yihV"
                     /locus_tag="OO179_003183"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5533156.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5356..6252
                     /gene="yihU"
                     /locus_tag="OO179_003184"
     CDS             5356..6252
                     /gene="yihU"
                     /locus_tag="OO179_003184"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5533157.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6276..7154
                     /gene="yihT"
                     /locus_tag="OO179_003185"
     CDS             6276..7154
                     /gene="yihT"
                     /locus_tag="OO179_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5533158.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7171..8412
                     /gene="yihS"
                     /locus_tag="OO179_003186"
     CDS             7171..8412
                     /gene="yihS"
                     /locus_tag="OO179_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5533159.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8550..9452
                     /locus_tag="OO179_003187"
                     /pseudo
     CDS             8550..9452
                     /locus_tag="OO179_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9651..11687
                     /gene="yihQ"
                     /locus_tag="OO179_003188"
     CDS             9651..11687
                     /gene="yihQ"
                     /locus_tag="OO179_003188"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5533160.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11733..13107
                     /locus_tag="OO179_003189"
                     /pseudo
     CDS             11733..13107
                     /locus_tag="OO179_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13150..14553
                     /locus_tag="OO179_003190"
     CDS             13150..14553
                     /locus_tag="OO179_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5533161.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14621..>14947
                     /gene="ompL"
                     /locus_tag="OO179_003191"
     CDS             14621..>14947
                     /gene="ompL"
                     /locus_tag="OO179_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5533162.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3670 a         3852 c         3727 g         3698 t
ORIGIN      
        1 tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta atgagattaa
       61 tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca acataatgat
      121 tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc cattattttt
      181 gcataatatt tgtgagctat agattttaaa aatatttatg aaaaaagtat ggcaccagag
      241 ggggttttga ctatcaaaaa tcgctcaaac ctgaactaat atgatgagtt aagcattatc
      301 tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac agtgatgtag
      361 caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt caatataacc
      421 ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc accgcccctc
      481 tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc
      541 agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttcgacc
      601 tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca
      661 ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg gatatgcgca
      721 tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca gataagcccc
      781 caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg gtgcggattc
      841 tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg ttgaatacgc
      901 acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac
      961 cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat caaaaaatgg
     1021 cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc cagcttggcg
     1081 aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc ttcctgacgc
     1141 gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg cacgtccggg
     1201 tgaacgacca taaagcgaat ggacgcttca ttgtcggcat taatatacaa tcggcctacc
     1261 gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg
     1321 tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca gcgaaactga
     1381 tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat agcgatactc
     1441 tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg
     1501 ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt tgcgtgttca
     1561 tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg
     1621 atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg agggtaaact
     1681 gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc ttgccttcgg
     1741 catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct ttctgttcgt
     1801 cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg cccgtcactt
     1861 ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc
     1921 gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag tgacagtaat
     1981 ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat
     2041 caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat aaagcgcgaa
     2101 acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa tcgcgtcgcg
     2161 gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga tccacgacaa
     2221 cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg
     2281 cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg gccccagcgt
     2341 taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt tactgcgcca
     2401 gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga cgatcagccc
     2461 gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga tataccgctg
     2521 aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta actgaatgct
     2581 gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac
     2641 taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc
     2701 aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt gcctggcttt
     2761 gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct
     2821 ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc ggcgttatcg
     2881 tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg ctggtaaatt
     2941 cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc agcagcatca
     3001 cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt attggaaagc
     3061 accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc
     3121 agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag cggtagagcc
     3181 atctcatgac acagcgcctc tgcgaacgct tcatcgctaa tttccccacg ctcatgctga
     3241 tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat acgcgttaaa
     3301 tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt acctaaatca
     3361 aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg
     3421 atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa atctacgcgt
     3481 cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg
     3541 gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt tcaaccgccg
     3601 ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg tgcgccatca
     3661 tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca tcgctctcaa
     3721 tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag gacgccgctg
     3781 aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc atcacttcaa
     3841 agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt atccgcaaat
     3901 gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg gtgataaata
     3961 ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt tttttctcgg
     4021 tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac gctggaagcc cgacccgcgc
     4081 caccgtgatg gcgcgtaatc aggccttgct cgcttaattt acgaatatcc cggcggaccg
     4141 tctgcgtgga gacatccagc agatttgcca gctcatcaat attcatatac ccacgctcgg
     4201 cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt tagttcggta aggctcatga
     4261 aaattcctcg aaaaaccatc accctggcat tttatacaaa aagtgacaaa aaagatcggg
     4321 tttgatcaca gtcagggagc ccggctcgtc caccgggacg tgtgcatttt aacgccgcta
     4381 caccgctggc gaagcggact gactccgcta aatccccact tgttgccagc gccaccgcca
     4441 aagcgccgtg aaaaacatca cccgcacctg tggtatctac cacatcaact ttgaaggccg
     4501 gttgatgctg acgcccacca ttttccagcc agtcgcagcc tgcgctaccc tgggtcacat
     4561 agacatgacc atttgtgagc gtttgtgcct gttttagcgc actggccatc tctttcacgc
     4621 ccgttaagcg cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat gccaccagct
     4681 cactgatatc ctgcggcgta atgtccccgt ccagaacggt catcacaccc gcctgacggg
     4741 ccagggtgaa ggctttttta gcgccgtcgt gccagcgtac atctgccagc acaacatccc
     4801 actgagagaa atcaatttcc tccaaccact ctgcgtcagg cagcagatcc gggctggggt
     4861 agttaatgat tatccgctcg cctttggtat ccaccatgat ggcggattgc gaagatttcg
     4921 cctggttata ccgtttggtg taacgggtgt taacgcccca ggattccagc tctgccagca
     4981 ggctgttgcc cgcgtcgtca tcacctacgc gaccaataaa atcgacctgc gcccccagcc
     5041 ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc aacttccgta taatttctcg
     5101 ccacgtattt accgctctcc gtcggtaacc cttccacgta atagatgcga tccatcacgg
     5161 ttatacctac acaagcaaca cgaatcatgg tcattcctta aacattttgg gatgcgttca
     5221 ttttaatttc accaattgtt taaaaagtga cggctgtcaa ttttttgacg ataaattaca
     5281 aattcgatca aaaacagaca agcaaagcat gtcataaatg acaaaaagtg acatcactgt
     5341 attcaggaga gggttatggc agcaatcgcg tttatcggtt taggacaaat gggttcgcca
     5401 atggcgagca atttattgca gcaagggcat caacttcgcg tctttgatgt gaacgccgaa
     5461 gctgtgcggc atctggtaga caaaggtgcg actcccgccg ccaacccggc gcaggccgct
     5521 aaagatgccg aatttatcat taccatgttg ccgaatggcg atctggtgcg caacgtgttg
     5581 ttcggtgaaa acggcgtttg cgaaggctta tctaccgatg cgctggtcat tgatatgtcc
     5641 accatccatc cgctgcaaac cgataaattg attgccgata tgcaagccaa aggcttcagc
     5701 atgatggatg ttccggtagg ccgtacttct gcaaatgcca ttaccggtac tctgttactg
     5761 ctggctggcg gcaccgctga acaagttgaa cgtgccacac cgatcctgat ggcgatgggc
     5821 agtgagttga tcaacgctgg cggtccgggc atggggatcc gcgttaagct catcaacaac
     5881 tacatgagca tcgcgctcaa tgcgctttcg gcagaagccg ccgttttgtg cgaagccctg
     5941 aatcttccct tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg taaaggtcac
     6001 ttcaccactt cctggccaaa caaagtcctc agcggagatc tttctcccgc cttcatgatc
     6061 gatcttgccc ataaggatct tggcatcgcc cttgatgtcg ccaaccagct gcatgtgcca
     6121 atgccgctgg gggccgcctc acgggaggtt tatagccagg cgcgcgcagc gggtcacggt
     6181 cgccaggact ggtccgccat tctggaacag gtccgtgtca gtgccgggat gactgccaaa
     6241 gtaaaaatgt aacgactgga taaaggaata aatgaatgaa taagtacacc atcaacgaca
     6301 ttacgcgcgc atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca
     6361 tgatgtttgc cgcggctggg gcacccgctc cggtagccga tagcgtttta actgatttca
     6421 aagttaacgc tgcaaaggcc ctctcgcctt atgcctcggc gattctggta gatcaacaat
     6481 tctgctaccg ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg
     6541 ccgccgatga gttcattcct ggcaacggta ttccggtcga tagcgtggtt attgaccgca
     6601 aaatcaatcc gctacagatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt
     6661 ggcgtagtga tgaagatgcg cagcaacgtc tggatatggt gaaagagttc aacgaactgt
     6721 gccactcaca cggtctggta agcatcattg agccagtcgt ccgcccaccg cgtcgtggcg
     6781 ataaattcga tcgcgaacaa gcgatcatcg atgccgccaa agagctgggc gacagtggcg
     6841 ctgacctcta caaagttgaa atgccgcttt atggcaaagg tccgcaacaa gagcttctct
     6901 gtgcttcaca acgtctgaat gaccatatca atatgccatg ggtgatcctc tcttccagtg
     6961 tcgacgaaaa actgttcccg cgtgccgtac gcgtggcaat gacggcgggc gcatcgggat
     7021 tcctggcagg ccgtgcagtc tgggcatcgg tcgtcggttt accagacaac gagctgatgc
     7081 tccgtgacgt ttgcgcaccg aaattacaac aacttggcga tatcgtcgac gaaatgatgg
     7141 ctaaacgccg ctaagatgag gataaaagga atgaaatggt ttaacaccct aagccacaac
     7201 cgttggctgg aacaggaaac cgaccgcatc tttgattttg gtaaaaattc cgtagtgccg
     7261 actggttttg gctggttagg caataaaggg caaatcaaag aagagatggg cacccatctg
     7321 tggatcacag ctcgtatgtt gcacgtttat tccgttgctg cggcgatggg tcgacctggc
     7381 gcttacgcgt tggttgatca cggtattaaa gccatgaacg gcgcactgcg tgataaaaaa
     7441 tatggcggct ggtatgcctg cgtgaatgac gagggcgtgg tggatgcctc caaacagggc
     7501 tatcaacact tctttgctct gctgggtgcc gccagcgccg tcacaacggg tcacccggaa
     7561 gcgcgcaagc tgctcgatta caccattgag attatcgaga aatatttctg gagcgaagaa
     7621 gagcagatgt gcctggagtc ctgggacgaa gccttcagca aaaccgaaga gtaccgcggc
     7681 ggcaatgcca atatgcacgc ggtggaagct ttcttgattg tttatgacgt cactcacgac
     7741 aaaaaatggc tggatcgcgc gattcgcgtg gcttccgtga ttatccacga cgtcgccaga
     7801 aataatcatt atcgcgttaa cgaacacttc gatacccagt ggaatccgct gccggattac
     7861 aacaaagata acccggcgca tcgcttccgc gcgttcggag gtacaccagg ccactggatc
     7921 gaatggggcc gtttaatgct gcacatccat gctgccctgg aagcccgttg cgaacaacca
     7981 ccagcatggc tgctagaaga tgccaaaggt ctgtttaacg ccaccgtgcg cgatgcctgg
     8041 gcacccgatg gagcggacgg gattgtttat accgttgact gggaaggaaa accggtggtc
     8101 cgcgaacgtg tacgttggcc tatcgtcgaa gcaatgggta cggcctacgc gctctacacc
     8161 gtcactggcg atcgccagta tgaaacctgg tatcaaacat ggtgggagta ctgcattaag
     8221 tacctgatgg actatgaaaa tggttcctgg tggcaggagc tggatgcgga caataaggtc
     8281 atcaccaaag tctgggacgg caaacaggat atttatcacc tgctgcattg cctggtgatc
     8341 ccgcgtatcc cgttagcgcc cggcatggct ccagcggttg cagcgggtct gctggatatt
     8401 aatgcgaaat aactgatctg aaaatagggt gcgggttttc ccctcaccct aaccctctcc
     8461 ccaaaggggc gaggggaccg tccggtgcag tttttgggtt tgacatcagc ctcacgggag
     8521 gctgtatgtc gttaattaag gtgccttgta tgcaaatcac caatatgcat tgcagtggac
     8581 agaccgtttc acttgccgcg ggggattatc acgcgaccat tgtgaccgtc ggtgctggtc
     8641 tggctgaatt aacctttcag ggttgtcatt tagtcatccc gcacaaaccc gaagagatgc
     8701 cgctggcgca tctgggtaaa gtgcttattc cgtggccgaa ccggatcgcc aacggctgtt
     8761 accgttatca ggggcaggaa tatcagttac ccattaatga acatagttca aaagccgcta
     8821 ttcacgggct gcttgcctgg cgagactggc aaattagtga attaaccacc accagcgtta
     8881 ccctgacagc ttttctgccg cccagttacg gctatccgtt tatgctggct tcacaagttg
     8941 tttattcgct caacgcacac acgggtcttt ccgttgagat cgcaagccag aatatcggca
     9001 ctgtcgcagc gccttatggc gtgggtattc atccttattt aacctgcaac ttaacatctg
     9061 ttgatgaata tctgttccag cttccggcaa actaggttta tgccgtcgat gaacacgtaa
     9121 atccgacaac gcttcaccac gttgatgagc tggatttaaa tttcacgcag gcgaaaaaaa
     9181 tagccgcgac aaaaatcgac cacaccttta aaaccgctaa tgatttgtgg gaaatgacca
     9241 ttacccatcc acagcaggcg ctttcggtaa gcctttgttc cgatcaaccc tgggtgcagg
     9301 tttatagcgg tgaaaaatta cagcgtcagg gactggcagt tgaacctatg agttgcccgc
     9361 ctaacgcctt taattcaggt atcgacttat tattactgga accaggaaaa ccgcaccgcc
     9421 tgttttttaa tatttacgga cagagaaaat aacttgtatt gattagcagt caaaatcatt
     9481 gaagaacatt tcactcgcta aaggcaacat tatgtatctt caatttatta agttaaaaac
     9541 agctcagctt ctgtaaagtt tttatatatc agttttatga gaatcatttt acataagcct
     9601 gtaataacag gaaggcaaca atctcgttct caactctatg gagattagtt atggatacgc
     9661 cacgtccaca gttattagat tttcaatttc atcagaataa cgacagtttt accctacatt
     9721 ttcaacaacg tcttatttta acccataaca aagataatcc ttgtttatgg attggctcag
     9781 gtatagcgga tatcgatatg ttccgcggta atttcagcat taaagataaa ctacaggaga
     9841 aaattgcgct taccgacgcc atcgtcagcc agtcaccgga tggttggtta attcatttca
     9901 gccgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat
     9961 tgctggaact acaaaacgac aaccttaacc acaaccgtat ctggctgcgc cttgccgctc
    10021 aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgcggca
    10081 aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaaacaa acctatgtca
    10141 cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta ttactggact ttcttcccac
    10201 agcctacgtt tgtcagcacg cagaagtatt actgccatgt tgataacagt tgctacatga
    10261 acttcgactt tagcgccccg gaataccatg aactggcgct gtgggaagac aaagctacgc
    10321 tacgttttga atgtgctgac acatacatct ccctgctgga aaaattaact gccctgctgg
    10381 gacgccagcc agaactgccc gactggattt atgacggggt aacgctcggc attcagggcg
    10441 ggacggaagt gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg aaggttaacg
    10501 gcatctgggc gcaggactgg tctgggatcc gtataacctc ttttggcaag cgcgtgatgt
    10561 ggaactggaa gtggaacagc gaaaactacc cgcaactgga ttcacgcatt aagcagtggt
    10621 atcaggaggg cgtgcagttc ctggcctata tcaacccgta tgttgccagc gataaagatc
    10681 tctgcgaaga agcggcacaa cacggctatc tagcaaaaga tgcctctggc ggtgactatc
    10741 tggtggagtt tggcgagttt tacggcggcg ttgtcgatct cactaatcca gaagcctacg
    10801 cctggttcaa ggaagtgatc aaaaagaaca tgattgaact cggctgcggc ggctggatgg
    10861 ctgacttcgg cgagtatctg cccaccgaca cgtacctgca taacggcgtc agtgccgaaa
    10921 ttatgcataa cgcctggcct gcgctgtggg cgaagtgtaa ctacgaagcc cttgaagaaa
    10981 cgggcaagct cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt agccagaaat
    11041 actccaccat gatgtgggcg ggcgaccaga acgtggactg gagtctcgac gatggcctgg
    11101 cgtccgtagt cccggcggcg ctgtcgctgg caatgaccgg acatggtctg catcacagcg
    11161 acattggcgg ttacaccacc ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct
    11221 ggtgcgattt cagcgccttc acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg
    11281 acaactggca gtttgacggc gatgcggaaa ctatcgccca ttttgcccgt atgactaccg
    11341 ttttcaccac cctgaaacct tatctgaaag aggccgtcgc gctgaatgcg aagtccggtc
    11401 tgccggtcat gcgcccgctg ttcctgcatt acgaagacga tgcacatacc tattccctga
    11461 aatatcagta cctgttaggc cgcgacattc tggtcgctcc ggtacacgaa gaaggtcgca
    11521 gcgactggac gctctatctg ccggaggata actgggtaca tgcctggacg ggtgaagcgt
    11581 tccggggcgg ggaagttacc gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg
    11641 ccgatagcga atgggcggca ctgttcgcgt cgttaaacag catctaatca catccgcccg
    11701 tggggaaact catgggcaaa gggagaacat cgatgagtca catcacaacg gaagatccag
    11761 caactttacg cctgcccttt aaagagaaac tctcttacgg tattggcgac ctggcctcta
    11821 acatcctgct ggatatcggt acgctttatc ttttgaagtt ttataccgac gttctggggc
    11881 tgccaggcac ctatggcggc attatctttt tgatttcaaa attctttact gcgtttaccg
    11941 atatgggtac cggcattatg ttggattccc gacgcaagat cggtccaaaa ggtaagttcc
    12001 gtccttttat tctgtatgcg tcattcccgg tcaccttact ggcgatcgcc aactttgtcg
    12061 gcacaccgtt tgatgtcacc ggtaaaacgg tgatggccac tattctgttt atgctttacg
    12121 gactgttttt cagcatgatg aactgctcct acggcgcaat ggtcccggca atcaccaaaa
    12181 accccaacga acgcgcctca ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc
    12241 tgctgtgcac ggtgggattc gtgccagtta tgaatcttat cgaaggtaat cagaaacttg
    12301 gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct
    12361 actcaggcgt gaaagagcgt tatgtcgaaa cccaaccaac caatccggcg caaaagcctg
    12421 gcctgttgca atctttccgc gcaattgccg gtaaccgccc actgttcatt ctgtgtattg
    12481 ccaacctctg caccttaggg gcgtttaacg tcaagctcgc cattcaggtc tattacaccc
    12541 agtacgtact taacgatccc atcctgttgt cgtatatggg atttttcagc atgggctgta
    12601 ttttcatcgg cgtgttcctg atgcctggcg cagtcagacg ttttggtaag aagaaggtct
    12661 atatcggcgg cctgctgatt tgggtgctgg gcgatctgct caactatttc ttcggcggcg
    12721 gctcggtcag ctttgtggcg ttctcctgcc tggcgttctt cggctcagcg cctgaactgg
    12781 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
    12841 acggtctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
    12901 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
    12961 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
    13021 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
    13081 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
    13141 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
    13201 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
    13261 ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
    13321 gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
    13381 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
    13441 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
    13501 attgccacca cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
    13561 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
    13621 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
    13681 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
    13741 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
    13801 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
    13861 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
    13921 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
    13981 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
    14041 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
    14101 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
    14161 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
    14221 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
    14281 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
    14341 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
    14401 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
    14461 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
    14521 acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
    14581 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
    14641 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
    14701 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
    14761 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
    14821 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
    14881 aaccatccag cccggtggct taattaatga taagagtatc agttcgggtg gtgcagtgta
    14941 tctggac
//
LOCUS       DAJTNW010000092        14941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNW010000092 DAJTNW010000000
VERSION     DAJTNW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..494
                     /locus_tag="OO179_003192"
     CDS             <1..494
                     /locus_tag="OO179_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5533163.1"
                     /translation="MTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYD
                     WIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKN
                     YLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFS
                     SVK"
     gene            549..1094
                     /locus_tag="OO179_003193"
     CDS             549..1094
                     /locus_tag="OO179_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5533164.1"
                     /translation="METGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVEHLCPLCIA
                     DGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGWQQ
                     EFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDCQG
                     YLFRCLHCGKLRLWGDYFVVI"
     gene            complement(1148..1870)
                     /locus_tag="OO179_003194"
     CDS             complement(1148..1870)
                     /locus_tag="OO179_003194"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5533165.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1872..3043)
                     /locus_tag="OO179_003195"
                     /pseudo
     CDS             complement(1872..3043)
                     /locus_tag="OO179_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(3070..4686)
                     /gene="bglH"
                     /locus_tag="OO179_003196"
     CDS             complement(3070..4686)
                     /gene="bglH"
                     /locus_tag="OO179_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5533166.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4772..6166)
                     /gene="bglB"
                     /locus_tag="OO179_003197"
     CDS             complement(4772..6166)
                     /gene="bglB"
                     /locus_tag="OO179_003197"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5533167.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(6185..8062)
                     /gene="bglF"
                     /locus_tag="OO179_003198"
     CDS             complement(6185..8062)
                     /gene="bglF"
                     /locus_tag="OO179_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5533168.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8196..9017)
                     /gene="bglG"
                     /locus_tag="OO179_003199"
     CDS             complement(8196..9017)
                     /gene="bglG"
                     /locus_tag="OO179_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5533169.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(9304..10029)
                     /gene="phoU"
                     /locus_tag="OO179_003200"
     CDS             complement(9304..10029)
                     /gene="phoU"
                     /locus_tag="OO179_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5533170.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(10044..10817)
                     /gene="pstB"
                     /locus_tag="OO179_003201"
     CDS             complement(10044..10817)
                     /gene="pstB"
                     /locus_tag="OO179_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5533171.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10909..11799)
                     /gene="pstA"
                     /locus_tag="OO179_003202"
     CDS             complement(10909..11799)
                     /gene="pstA"
                     /locus_tag="OO179_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5533172.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11799..12758)
                     /gene="pstC"
                     /locus_tag="OO179_003203"
     CDS             complement(11799..12758)
                     /gene="pstC"
                     /locus_tag="OO179_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5533173.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12845..13885)
                     /gene="pstS"
                     /locus_tag="OO179_003204"
     CDS             complement(12845..13885)
                     /gene="pstS"
                     /locus_tag="OO179_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5533174.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(14133..>14941)
                     /locus_tag="OO179_003205"
     CDS             complement(14133..>14941)
                     /locus_tag="OO179_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5533175.1"
                     /translation="KINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEY
                     HANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRD
                     TPVDYSQPMADVYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKIT
                     KTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGG
                     ELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3864 a         3799 c         3441 g         3837 t
ORIGIN      
        1 aaatgactca ggaagcgctc agacggatag cagagttata cgacatagaa gcggagatac
       61 gtggcagtcc ggcagaggaa cggcttgcag tcagaaaagc cagaagcgtc cagttgatgc
      121 agtcgttgta cgactggata cagttgcaga ggaaaacgct gtcgaaacat gcggagatgg
      181 cgaaggcgtt cgactatatc ctgaatcact ggaatgcgct gaacgagttc tgtcgtgacg
      241 gctgggtgga aatagacaac aacatcggtg aaaacgcgtt acgatcggtg gcggttggaa
      301 gaaaaaatta tctctttttc ggctcagaca agggaggaga aagtgcggcg atcatctaca
      361 gtctgctggt cacctgcaaa cagaacgaag tggagccgga ggactggttg cgcgaagtga
      421 tcgagaagct caatgactgg ccgtcgaacc aagtgcatga actgctgccc tggaacttct
      481 cgtctgtaaa ataatcctta cgctacgtac ttttcggggc gcttacattc aaatatcatc
      541 ccaagccact ggaaacaggc gcatttgaac aggataaaac cgtagagtgc gattgctgtg
      601 aacaacagac gtcagtttat tactcgggtc ccttttattg cgttgatgaa gttgaacatc
      661 tctgtccgtt gtgtattgcg gacggttctg ctgcagaaaa gtttgcaggt agttttcagg
      721 atgatgccag catagaaggt gttgaatttg agtatgatga agaggacgaa tttgccggta
      781 ttaagaacac atatcctgat gaaatgctga aagagttggt tgaacgcacg ccaggttatc
      841 atggatggca acaggaattc tggcttgcgc attgtggcga tttctgtgct tttatcggct
      901 atgtgggctg gaatgatata aaagatcgcc tcgatgaatt tgccaacctt gaagaagatt
      961 gtgagaattt cggtattaga aattctgatc tagctaaatg cctgcaaaag ggtggtgatt
     1021 gtcagggtta tctcttccgc tgtctccact gcggcaagct gagactgtgg ggtgattatt
     1081 tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatacatcc ggccttattt
     1141 cttcagctta accgaacgct aattctgcgg cagcggcttt atcagcgatt accatcagcg
     1201 aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct tgcagcacat
     1261 ttttcagtgc ctgcgcttta cctgcgccac tgacaattat cagcaagttt ttcgccgcca
     1321 tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag tcgccgccca
     1381 actcaccatg cgccacaata tcaaccatct ctccctggat cgggaactcc acggtttgct
     1441 catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca cctaatccca
     1501 gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg cggtagttat
     1561 caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa aacagattac
     1621 gcagattggt aatcgttacg ccttctccct ctttgccgcg aaagggaatt tcatcgaaat
     1681 tatagaaata gcagttatcg taccaaggct tacctttaac cagggtggtg aggtattcat
     1741 acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc gtcttcgaca
     1801 tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct tcggtaatga
     1861 ttaatttcat cttaatgcct tattttaaat ttctgtacga aggctgcata ggcaggccgc
     1921 cagacatcca tttcatggtt caggccggga tattcctggt aatcaaactt aatttttttc
     1981 tgctcaagct cagttttcag cccggcgata tccttgccgg ttacgacatc tttatcacca
     2041 acgaccacag taaaattacg tagttgctgg ttaatagctg ccggatcgtt cagccgggcc
     2101 gcgacacctt catccggtac ggttgtcgtg gtaacaccac tgaatgtggc cagccaaagc
     2161 tttccagatg attcattccg gaaaccagcg cctggtaccc gccttgtgaa agccctgcca
     2221 gcgcgcgacc atcggcatct ttacggacac taaaacgctt gctaatcagc gggataatat
     2281 cgttcatcag ttcgcgatct gccgctttag cattcagcgg ataaaagact ttacgtcttt
     2341 cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta tccgggatca
     2401 ccaccagcat cggtttaatt ttcccttcag ccagcaggtt atccatgatt tgcgggatac
     2461 gcccctgatc gatagcggaa cgtccggtat caccaaaacc gtgatagaaa tagagcactg
     2521 gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca gacatacatc tgacgttcag
     2581 attgcaatgc gttggagtgg taagttatgg cgatcaaatc accatgcgca acagaacgcg
     2641 tatccagata actgcctggc accagaatca tactggagtt aacctggcgc tgaggcttag
     2701 tcattgcggt gcctgtatca atgctgcgta caccatcaac attgaaaaaa tactcgtaca
     2761 ggttgccttt caggataggt gtgcgccacg accagactcc tgcttcgtct ttggtcatcg
     2821 ggtgaatatt gtccggaacc ggaacaccca ccactacaga gacatttttt gccccagggg
     2881 caaagtagcg aaaggtcacg ctgttatcgg cattgacctg agtcacatac tgtttcaccg
     2941 ggatagtggg tgccgggctg gctggcatat ccgctgctat ggcccactga gcagaaatac
     3001 cgctggcaat agccagcgtt aaagcagcaa tttttatatt catcgtaata ttcctttcat
     3061 taagttccat taccaccaga tttcagcctg ggcaccgaca gcaaactggg cgtctttatt
     3121 gtcttcgaag gtgaagccat ccagttcgtt ttccagggct ttgatatacg tggcgtagaa
     3181 acgaatttcc agacgcgagg tcaacatact ggtattgact ttaaaggtat ggaagagcgt
     3241 ggttttataa ccagactcat taaatttatt actattcgca tctttgtttt gctgggtgaa
     3301 atagcccagt tcaacacctg tctggttata ttggtcccaa atataggccg ggcgaacaac
     3361 cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg tagctataaa tattgttacc
     3421 gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg ccgatatagg cttcgccctg
     3481 cgaggtcaga cgaactgctg ttccgccggt gtgatcacca taataacgac cattaaaggt
     3541 ggtaaatgga cttgcgccag cataacggcc aaagttcctg gcgatagagt tattcgcaac
     3601 caggaaggag aattcgttga agccaccttt atcaaatttc tgcgttaaag atgtgccgaa
     3661 catccaggta tctttccagt cataataccc gttattatcc tgattatctt tttccgatgc
     3721 gctttcgttt gccgtgacat aacgaccact caccattaag gtcgctttat cccataacgg
     3781 gatatcttta tagcgtaaat caatagtatg ggtattaatc tgctgtttgt tttgcaggct
     3841 gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata tcaattttac ccggaccgac
     3901 tttccagttt tccagaccta cacccgctgc agcatcagta cgctgcgttt tccagtccag
     3961 catctggatt tcaattttcg gcgcaccgtg tttacccacc cagaaatcag cctctggcgc
     4021 aaagggcagg aaacctttgg tggtaacgta catatcggag aactgcataa agttctcgcc
     4081 accggcatta tcgccaaacc agccggtaga gtactgctga ccaacgttac catctatcat
     4141 cacaacggca tcaacccgtt tgccgttttc gttgtagaca cgttgtttta actgcaaatc
     4201 aaaccagccg gagtattcgt taccaaagcg gcccagagaa ccaatcgccc atgatttagg
     4261 tgaaccatga gaggcggtcc cccaaccaga acggtaatag ccgttgtaac taaagccaat
     4321 ttcatctttc acaaacttac tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt
     4381 ctcactggca tttttctctt catttttgac cagtacagca tcaggttttg cggcactact
     4441 ggtcgggaac ggattggcgg catacccttg atcattcgta cttacgctac gattcaccgt
     4501 cgctggcgta tattttttct tctcttcatc tttatacttt ttgagttcgc tttgcgtttc
     4561 tcttaacgct ttttctaata actcaaggcg ttgttccacc gttaatgatt cagccaatga
     4621 ttgtgcagaa aaggctaacg gtgccattaa taagatggca gaggtaataa gatttcgtct
     4681 aaacataata tccctttatg gtgcaaagaa agaattaacg tatcgcatcc agactgttct
     4741 gaatgcgacg ataattaagg tgctttattg gttatttttt taatgacagc ccccgcgttt
     4801 tgatcacttc tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc
     4861 cattatcatc acgatccaca taaataaagc cgtagcgctt agacatttgt gaatgagatg
     4921 cactgactaa atcaattggc ccccaactgg tgtaccccat aatatccaca ccatcggcaa
     4981 tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc aattcgataa tcgtcctgta
     5041 tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc attctcgaca ataaataacg
     5101 gtttttgata acgatcccaa agcgtattta acagaacccg taatccaacc ggatcaattt
     5161 gccaccccca ctctgaactt ttcagatgcg gattggggat catattcagt atgttgccct
     5221 gcgcattttt attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag
     5281 agatgaaatc tacagtatgt tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga
     5341 tattgtggtc gcggaagaaa cgctgcatat agccgggata ttggccacgc gcctgaacat
     5401 caccaaagaa catccagcgc cggttctctt ccatggcctg caacatatcc tgtggctggc
     5461 aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc gattttcgct tcggggatca
     5521 ggctatgata ggctttaact gcccgcgcac tggcaaccag ttgatgatgg atcgcctgat
     5581 aaacttccgc ctcgccactc tcttctgcca gccccacgcc cgtgaatggc gcgtgtaacg
     5641 acatattgat ttcattaaac gtcagccata acgctacttt atgttggtag cgggtaaaga
     5701 ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg attagcccaa ccgccgtagt
     5761 ttttcaccag cccatatagc atttcgtaat gggataacgt taccagcggc ttgatccccg
     5821 cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca ttcggttcgg
     5881 cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc
     5941 ccatctcggc aaataacgcg atatcttccg ggtaacggta ataaaaatcg atggcgacat
     6001 ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccattacg ccatgaggct
     6061 gtaaatctga ggtcgtgatc cctttgccat cttccagcca ggcaccttcc acctgattgg
     6121 cagctgttgc accaccccaa agaaatgttt ctggaaatgc tttcataatt aactcctttt
     6181 atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg
     6241 gtaatacgtc tgtaaaatca tcgctattac tgattaatac cggcgtcgtc agatcaaatc
     6301 cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt
     6361 cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga
     6421 catgaatcag gatctccaca ccatcatctg actcaatgcc aatggcgtgt aatgtggcga
     6481 acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg
     6541 cgataccttt acccaacagg ccactggcaa acgtggtatc agcgacgtga atgagcggca
     6601 caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg
     6661 gtgttttctc ttgcggggca ccctgcgctg gctgacgttt actggtgatg aaatgaagca
     6721 tcaacgtacc aacaaatgcg caacctatgg caatgacacc gccaataacg ctggcccaga
     6781 cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa ggtgaaaata cttggcaaac
     6841 caaaggagta aactttcgtt tgcgcgtagc caatgatggt ggcccccaat gctccactga
     6901 tacaggcgat aacaaagggg tacttacgcg gcaggttgac gccatatacc gctggttcgg
     6961 tgataccaaa cagacccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc
     7021 gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg ggcattaaca
     7081 gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc
     7141 ccctgtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc gcgcccgcaa
     7201 atgcaggaac cgcctgataa agccagagat aaccggcggc aatcatttcg cttatccagg
     7261 ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga catagcaatg
     7321 gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc
     7381 tgcacaacca ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga
     7441 ccggaatacc caggaaatcc agccccggcg catccgcttt ttgcccgttc tcgaaggcag
     7501 tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc
     7561 cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa gaacccaaac aaggcatcac
     7621 tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg
     7681 ccagagccag catacctttc aggatcccgg ttgccgccat cagaccgatc agaggcgtaa
     7741 aaatacctga aataacataa acaaagcggt ttagcagatt acctttctca tcattttccg
     7801 gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt aaccgccagg aagacatcgg
     7861 ccacatggtt acctatgacc acctgaaact gaccaccgct ttccaccacc ataataatac
     7921 cgggggtctt tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta
     7981 atcgcgttgc gcaatgcatc agactcacaa tgttatctgc gcccccgact cctgcgacta
     8041 tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa
     8101 aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt
     8161 agtgcggtaa caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct
     8221 atatttatgg ctaaaaacat aatctctgcg ggtgaaattt tacgttgata ctgcaaacca
     8281 ataaaaatgg cgatccgttc cgcacattgc catgcttgcg ggtaattttg ttttactgct
     8341 tgttgtaatg attcatcact atcgttaatt gaagcatgtt caagaatacg ccaagataaa
     8401 aacttcagat gtgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg
     8461 aactgaaatt ttattaattg cagcatttcg cgcattaact gcgtgacacc tgcaacatcc
     8521 tccatatttc cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct
     8581 ttcggtaact gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg
     8641 aactctttcg ggtaaagccg ctggatatcc cacagcaaca cgttttgctg aaagcgttta
     8701 atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgtaa ttttcccaag
     8761 cgctcctgcg ctaaagagat aagacgatca caggttgcca tcacctcaag aggaatatga
     8821 cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata ctctttttct
     8881 attccacttg agttaattct ttcgccagcg cgtttttgaa agccaattcc gcgccccatg
     8941 acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt gagaattttg
     9001 gtgatttgca tgttcatagc aaggaccttt tttataaaca aaaaaaaacc gacttcacca
     9061 gtattctctg gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcatttatta
     9121 atggttttta taacgaacat ccaggttcgg aaattaattt aattgcgtgc agagaaaata
     9181 gcaatgcgct attgataaaa atatgaccat gctcgcagtt attaacttta tgtaatttta
     9241 ggaatttata aagttatata taacaaatcc caataattaa gttattggga tttgtctggt
     9301 gaattatttg tcgctatctt ttcccgccag cagtttatcc agctcatcac cgcctacatg
     9361 acggaaatcc tgacccttca cgtagtagaa gataaactca caaatattct ggcagcggtc
     9421 gccgatacgt tcgattgaac gcgcacagaa cagcgcggta agtacgctcg gaatagtgcg
     9481 cgaatcttcc atcatgtagg tcatcagttg acgaacaata ccttcgtatt cctgatcgac
     9541 ttttttatct tcacgataaa tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc
     9601 cagcacgtcg tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa
     9661 cggctgatgc tgctgggaga atttctccag cgcagtacgg cagattttgt ccgccacgtc
     9721 gccaatacgc tccagctcgg caatggtttt actgatcacc ataaccagtc gcaggtcgct
     9781 cgccgtcggc tgacgtttgg cgataatgcg cacgcaggct tcatcgatcg ccacttccat
     9841 catgttgacg ttcttgtcgc cttcgatcac gcgcttcgcc agatcgctgt cctggttatg
     9901 catcgcggtg attgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg
     9961 cgtgcggata ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag
    10021 actgtccata acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt
    10081 ttcttcgctg gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg
    10141 tacataaatg ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtgacgatc
    10201 accacggtgt aatcctgctt cagttcggtg atcagctctt caatacgccc ggtagagatg
    10261 gggtcgagcg ccgaacacgg ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca
    10321 cgcgcaatac acagacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat
    10381 ttatctttgg tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc
    10441 atgtcggcac aggagagctt ctcaaacaga cgaacgccaa aggcgatgtt gtcgtagatg
    10501 gacatcggga acggcgtcgg tttctggaac accatgccca ctttcgcacg cagcagggcg
    10561 atatcctgag agttggtcag gatgttgtcg ccatcaagca gaatttcacc ttccgcacgc
    10621 tgctccgggt acagttcaaa cattttgttg aaggtacgca gcagcgtcga tttaccgcag
    10681 ccggacggcc cgataaacgc cgtaacctgg tttttggcga tatccaggtt gatgtttttc
    10741 agggcatgga atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca
    10801 gtttcaacca tactcattgc aatcttttcc tcatcgtacc tgatgctttt cgctaatcag
    10861 gcttaccgta gggccgggta aggcgttcac gccgcatccg gcaaagtgtt aaccgtgttt
    10921 attcttcgca aaaacaacgc gcgccagaat gttcagcagc aggacgcaca gggtaatgat
    10981 caataccccg gcccaggcca gttgttgcca ttccgcaaac gggctcatcg caaacttaaa
    11041 gatcgtcacc ggcaggttgg cgatcggctg catcatgtcc gtgctccaga actggttgga
    11101 gagcgcggta aacagcagcg gcgcggtttc accggcaata cgggcaatcg ccagcaggat
    11161 accggtcata atcccggaca ccgacgcttt cagcgtaatc gcggagatca tcttccactt
    11221 cggtgtaccc agcgcataag ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt
    11281 ctcagtggtg cggataacga tcggcacctg caacagcgcc agcgcaatta cgcccgccca
    11341 gccggagaag tgctccatct gtgccaccac gatggtgtag acaaacagac caaccacaat
    11401 cgacggcgcg gagagcagaa tgtcgttaat gaagcgaatc acttctgcca gccaggattt
    11461 acgaccatat tccgccagat aaatccccgc cataatgccc agcggcgtac cgaataccgt
    11521 ggcccacaaa attaacagcc cgctacccgc cagggcgttc gccagaccac caccttccgt
    11581 attgggcggc ggcgtcattt cagtgaacag cgccagcgac ataccgtcga taccgcgagt
    11641 gatggtggac attaaaatcc agatcagcca gaacaggccg aaggccatcg tcgccatcga
    11701 gagcgtcagc gcaatacggt ttttgaggcg gcgacgcgcc tgcattttgc ggcgagattc
    11761 agccagcgcc gcagtggttt gcatttcaac catagccatt agcgtgcccc ctcattctta
    11821 gccaggcgca taatcataaa cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg
    11881 atcaggccca gttccatcag tgcggcaacg tgcagaccgg attccgcttc cgcaaattcg
    11941 ttcgccagcg cagaggtgat actattgccc ggcatgtaca gcgaggcgct gtcgagctgg
    12001 taggtgttac cgatgataaa ggtcaccgcc atggtttcac cgagcgcgcg gcccagcccc
    12061 agcatgatgc cgccgataac accatttttg gtgaacggaa gaacgatacg ccagataact
    12121 tcccaggtgg tgcagccaat accgtaggcc gactctttca tcatcaccgg ggtttgttcg
    12181 aacacatcac gcattaccgc cgcaatgtac ggaataatca tgatggcgag gatcacgcct
    12241 gccgcgagga taccgatacc aaatgcgggg ccagagaaca gcgcgccaac aatcgggata
    12301 ttcgacatga tattgccgac cggctcctga aagtaaacgg cgaacagcgg cgcaaagata
    12361 aacaggcccc acatgccgta aacgatactt ggaatggctg ccagcagctc aatggcgata
    12421 cccagcgggc gtttcagcca gccaggcgca agctcagtca ggaacagggc gataccgaaa
    12481 ctcaccggga cggcgatcag cagcgcgata aacgaagtca ccaacgtacc gtagatcggc
    12541 accagcgccc cgtagatatc gttcggtgca tcccactctt tggtccatag gaaagccaga
    12601 ccaaatttct gaatgctcgg ccaggaggag atgatcagag agacaataat gccacccaac
    12661 atcaatagca caatcagcgc cgccagtttt accagcacgc tgaaaattat gtcgcccttt
    12721 ttacccggtg ggttaaaagc gggcttggtt gcagccataa gttactcttc agttaaacgc
    12781 gtttacaaag tcattttgta atgccggatg cggcgtaaaa caccgtaccc ggcctggagt
    12841 tttattagta cagcggctta ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa
    12901 cctgttcaac tacactatcc ggcaggctgg cgtaatccag gtcgttcgcc tgtttagccc
    12961 cggttttgta cgcccagtcg aagaatttca gcacctctgt gccttgttct ggtttcttct
    13021 gatctttgtg gatcagaatg aacgtggtag aggtaatagg ccatgcatct tcgcctttct
    13081 ggttggtcag atcctgagcg aaggttttgc tccagtctgc accttttgct gcattagcga
    13141 agttttcttc ggtcggacta accggtttac catcagcgga gatcagtttg gtgtacgcca
    13201 ggttgttctg cttcgcgtaa gcatattcaa cataaccaat tgcacccggc agacgctgaa
    13261 cgaacgcggc gataccgtcg ttacctttac cgcccagacc gatcggccat tttacggtag
    13321 agccagtacc aacgttgttt ttccactctt cgttcacttt cgccaggtag ctggtgaaga
    13381 cgaaggaagt cccggagcca tctgcgcggc gtactacagc aatgttttgt gaaggcagtt
    13441 tcagacccgg attcagtttg gcgatggctt catcatccca cttcttgatt ttgcccaggt
    13501 agatgtcgcc gagggtttta ccatccagca ccagttcgcc agacttcagc cctggaatgt
    13561 taaccgccag caccacgccg ccaatcacgg tcgggaactg gaacagacct tcctgagcca
    13621 gtttttcgtc agacagcggc gcgtcagagg caccaaaatc aacggtatta gcgataatct
    13681 gttttacgcc accggaagaa ccgataccct ggtagttaac tttattaccg gtttctttct
    13741 ggtaagtgtc agcccatttg gcatacaccg gcgcagggaa ggttgcacct gcacctgtca
    13801 ggcttgcttc tgcaaacaca gagaaagcac tcatcgataa ggtcgcggcg acaacagttg
    13861 cgacggtggt acgcataact ttcataatgt ctcctgggag gattcataaa gcattgtttg
    13921 ttggctacga gaagcaaaat aggacaaaca ggtgacagtt atatataagg aatatgacat
    13981 ttttatgaca gaaaggaatt accaaataac ttactgattt taaatgtgaa gatgaaaatc
    14041 ccacatgcca tctttttgtc atgatgcatt taaaagtgat aaagaaatgg gtgagatgac
    14101 aacgatgtga tgaacaatcg ttgtcagacc tattagcgca caatctcaac agttatcgtc
    14161 gctgttgcag taaattgccc cggggctggg cgagctccag taaggctcgc aggagcagct
    14221 gaaaaagtta cactacctga tttttcacca tacactgttg atttatccat gtctgttggc
    14281 aactcaccac cgttcacttt gatttcctta ttatttttgt cgtaaatttt tatccccacg
    14341 ttagagtttg aagttacgac cattgaattg tccgtattgg gttcggtagc ataaaaagac
    14401 gtactgatat catcggtatt cagtatcccc aggttttcac actcaatagt gactgtttta
    14461 gtaattttat gtgttgttac cgcacttcca acaacagatg aaaaatctgc agctggtatt
    14521 tctttaaaat caaaacaaac tggctttaaa ttatttattt cgcaactttg cggagctgta
    14581 atatcgcccc gaatataaac atcagccatc ggctgtgagt aatccaccgg ggtgtcacgg
    14641 ctaattgttc catacaatgt cactatcttt gtttcaggaa taatcaattt tcctgcaaat
    14701 gtcttcttga tataaaaata tactatcgct ttagccccac tggatatact taaaggttca
    14761 atgcggttgc aattgtactt tgtattacca gacgggttgt tagcatgata ttcaaaaggt
    14821 gcattcacat acccaacccc aagaatatct atagcaatcc caacatcaag gttcggatta
    14881 agatcataat aattaatatt agagtgaacc ccagcataag ttaatgcctg gtttattttt
    14941 g
//
LOCUS       DAJTNW010000093        14844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNW010000093 DAJTNW010000000
VERSION     DAJTNW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO179_003206"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO179_003206"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5533176.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO179_003207"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO179_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5533177.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OO179_003208"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OO179_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5533178.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="OO179_003209"
     CDS             2977..3060
                     /locus_tag="OO179_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5533179.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OO179_003210"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OO179_003210"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5533180.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OO179_003211"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OO179_003211"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5533181.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="OO179_003212"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="OO179_003212"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5533182.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="OO179_003213"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="OO179_003213"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5533183.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDIDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OO179_003214"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OO179_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OO179_003215"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OO179_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5533184.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="OO179_003216"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="OO179_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5533185.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OO179_003217"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OO179_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5533186.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="OO179_003218"
     CDS             13186..14385
                     /locus_tag="OO179_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5533187.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3698 a         3778 c         3910 g         3458 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatattgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tatt
//
LOCUS       DAJTNW010000094        14558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNW010000094 DAJTNW010000000
VERSION     DAJTNW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(337..582)
                     /locus_tag="OO179_003219"
     CDS             complement(337..582)
                     /locus_tag="OO179_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533188.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OO179_003220"
     tRNA            complement(1227..1302)
                     /locus_tag="OO179_003220"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OO179_003221"
     CDS             complement(1408..2115)
                     /locus_tag="OO179_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5533189.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OO179_003222"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OO179_003222"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5533190.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO179_003223"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO179_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5533191.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO179_003224"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO179_003224"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5533192.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OO179_003225"
     CDS             complement(7299..7574)
                     /locus_tag="OO179_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5533193.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO179_003226"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO179_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5533194.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OO179_003227"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OO179_003227"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5533195.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OO179_003228"
     CDS             9534..9860
                     /locus_tag="OO179_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5533196.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OO179_003229"
     CDS             10044..10763
                     /locus_tag="OO179_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5533197.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OO179_003230"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OO179_003230"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5533198.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="OO179_003231"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="OO179_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..14477)
                     /locus_tag="OO179_003232"
     CDS             complement(13164..14477)
                     /locus_tag="OO179_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5533199.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPK
                     LILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLL
                     IVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGC
                     SISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3812 a         3486 c         3741 g         3519 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    13621 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    13681 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    13741 gtaaaaatgc cgcccagaat gccgcccaga atgccgccca tcacaatgaa caccagaagc
    13801 aaacttggta aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa
    13861 agcgggtagc cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt
    13921 aagatccctg gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc
    13981 agggaaaaca caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag
    14041 acgtttacag cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt
    14101 gtgccaccaa cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata
    14161 ttcgccagga cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg
    14221 aggttaatca aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa
    14281 aaggggattg ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg
    14341 attgccgcga tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt
    14401 gagaacgaaa tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc
    14461 gcgatataac tatccattgg taatgctgcc aacttactga tttagtgtat gatggtgatt
    14521 ttaaggtgct tgcgtggctt ccatttccat cagatgtc
//
LOCUS       DAJTNW010000095        14515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNW010000095 DAJTNW010000000
VERSION     DAJTNW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(113..379)
                     /locus_tag="OO179_003233"
     CDS             complement(113..379)
                     /locus_tag="OO179_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5533200.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO179_003234"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO179_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5533201.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO179_003235"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO179_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO179_003236"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO179_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5533202.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO179_003237"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO179_003237"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5533203.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO179_003238"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO179_003238"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5533204.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO179_003239"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO179_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5533205.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO179_003240"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO179_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO179_003241"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO179_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5533206.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="OO179_003242"
     CDS             complement(12641..14095)
                     /locus_tag="OO179_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5533207.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14515)
                     /locus_tag="OO179_003243"
     CDS             complement(14092..>14515)
                     /locus_tag="OO179_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5533208.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3662 c         3429 g         3551 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagt gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaac
//
LOCUS       DAJTNW010000096        14240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNW010000096 DAJTNW010000000
VERSION     DAJTNW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..94)
                     /locus_tag="OO179_003244"
     CDS             complement(<1..94)
                     /locus_tag="OO179_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5533209.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGN"
     gene            complement(135..590)
                     /gene="csgB"
                     /locus_tag="OO179_003245"
     CDS             complement(135..590)
                     /gene="csgB"
                     /locus_tag="OO179_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5533210.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1343..1993
                     /gene="csgD"
                     /locus_tag="OO179_003246"
     CDS             1343..1993
                     /gene="csgD"
                     /locus_tag="OO179_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5533211.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1998..2387
                     /gene="csgE"
                     /locus_tag="OO179_003247"
     CDS             1998..2387
                     /gene="csgE"
                     /locus_tag="OO179_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5533212.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPCARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2412..2828
                     /gene="csgF"
                     /locus_tag="OO179_003248"
     CDS             2412..2828
                     /gene="csgF"
                     /locus_tag="OO179_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5533213.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2855..3561
                     /locus_tag="OO179_003249"
                     /pseudo
     CDS             2855..3561
                     /locus_tag="OO179_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3694..4185)
                     /locus_tag="OO179_003250"
     CDS             complement(3694..4185)
                     /locus_tag="OO179_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5533214.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4287..4841)
                     /gene="ycdY"
                     /locus_tag="OO179_003251"
     CDS             complement(4287..4841)
                     /gene="ycdY"
                     /locus_tag="OO179_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5533215.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4865..5602)
                     /gene="ycdX"
                     /locus_tag="OO179_003252"
     CDS             complement(4865..5602)
                     /gene="ycdX"
                     /locus_tag="OO179_003252"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5533216.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5657..6595)
                     /gene="ghrA"
                     /locus_tag="OO179_003253"
                     /pseudo
     CDS             complement(5657..6595)
                     /gene="ghrA"
                     /locus_tag="OO179_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6830..6917
                     /locus_tag="OO179_003254"
     tRNA            6830..6917
                     /locus_tag="OO179_003254"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6864..6866,aa:Ser,seq:gga)
     gene            complement(7067..7348)
                     /locus_tag="OO179_003255"
                     /pseudo
     CDS             complement(7067..7348)
                     /locus_tag="OO179_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7630..8634)
                     /locus_tag="OO179_003256"
     CDS             complement(7630..8634)
                     /locus_tag="OO179_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5533217.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8706..9266)
                     /locus_tag="OO179_003257"
                     /pseudo
     CDS             complement(8706..9266)
                     /locus_tag="OO179_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9351..9548)
                     /locus_tag="OO179_003258"
     CDS             complement(9351..9548)
                     /locus_tag="OO179_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533218.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9560..10051)
                     /locus_tag="OO179_003259"
     CDS             complement(9560..10051)
                     /locus_tag="OO179_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533219.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10048..10422)
                     /locus_tag="OO179_003260"
     CDS             complement(10048..10422)
                     /locus_tag="OO179_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5533220.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10469..10843)
                     /locus_tag="OO179_003261"
     CDS             complement(10469..10843)
                     /locus_tag="OO179_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5533221.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11006..11227)
                     /locus_tag="OO179_003262"
     CDS             complement(11006..11227)
                     /locus_tag="OO179_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5533222.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11290..11766)
                     /locus_tag="OO179_003263"
     CDS             complement(11290..11766)
                     /locus_tag="OO179_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5533223.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11782..12255)
                     /locus_tag="OO179_003264"
     CDS             complement(11782..12255)
                     /locus_tag="OO179_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5533224.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12597..13418)
                     /locus_tag="OO179_003265"
     CDS             complement(12597..13418)
                     /locus_tag="OO179_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5533225.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13518..13691)
                     /locus_tag="OO179_003266"
     CDS             complement(13518..13691)
                     /locus_tag="OO179_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5533226.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13811..>14240)
                     /locus_tag="OO179_003267"
     CDS             complement(13811..>14240)
                     /locus_tag="OO179_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533227.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3638 a         3484 c         3411 g         3707 t
ORIGIN      
        1 ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg gagaatacga
       61 ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg attgatttaa
      121 aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg tctctgcact
      181 acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc agaacctttc
      241 tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc gttggcactg
      301 cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt tgcccgattg
      361 ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg taactgagca
      421 ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga cttactcaat
      481 tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc ggctgcaatc
      541 ccaggcgcac ccagtattgt taacatcata aacaacaatt tgtttttcat gttgtcaccc
      601 tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta acgcaacctg
      661 tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt ttaataactc
      721 acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc cgcgtaaata
      781 cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt ccgctatcaa
      841 aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg tcttgcgcac
      901 aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg tgtggagaaa
      961 aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata tgtattatta
     1021 gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca aatgtacaac
     1081 ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata taatgagtta
     1141 cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt gaaatcagat
     1201 gtaatccatt agttttatat tttacccatt tagggctgat ttattactac acacagcagt
     1261 gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag gtgtgcgatc
     1321 aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg gtcatacatt
     1381 attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc ttaaacaatc
     1441 gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata tctcttcagg
     1501 ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt attggcagga
     1561 tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc ctgaagatta
     1621 cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg ccatggagga
     1681 tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct actttacgca
     1741 aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca gcacggaatc
     1801 agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg gcgcgtctaa
     1861 taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc atctttataa
     1921 tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg caaacgataa
     1981 cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca gaatttctgt
     2041 tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta actgaccata
     2101 ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg gaaagtgact
     2161 atacgggtaa cttaacgatt aatgaaaggc cctgtgcacg atggggaagc tggatcacaa
     2221 taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa agagacttcg
     2281 agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt cgccagataa
     2341 atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata aaaaattgtt
     2401 cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt tcgccattaa
     2461 gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt aacccaaata
     2521 atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat ccgagctata
     2581 acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag gccatccagt
     2641 cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc cgcatggtga
     2701 ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg aacgtgacag
     2761 atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat aactcaaccg
     2821 atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc ttttggttgc
     2881 cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca gaccgacatt
     2941 aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga cgggtaagat
     3001 ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct acccggcaag
     3061 taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct taacgagcgc
     3121 aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg aatcccgctg
     3181 caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta tgaaagcaac
     3241 gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac gcaataccag
     3301 ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga gatcctctcc
     3361 tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt tttccgcttt
     3421 attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga acccgttatg
     3481 ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga tggtatcgac
     3541 cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct ggtgaaatac
     3601 cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt aataatccgg
     3661 catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga tttcctgctc
     3721 acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt tcattgcgta
     3781 accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac cctgcccggc
     3841 aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca gctgtttggc
     3901 ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa tttccagatg
     3961 tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac taagcagggt
     4021 tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt aggccgtaca
     4081 gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc cccagatacc
     4141 ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc gcatgatagc
     4201 agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa attatctgtt
     4261 gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag ctcgtcccac
     4321 attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg cgtggttgca
     4381 tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc actgaacagt
     4441 gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga agccgcaagc
     4501 agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg ttcggaaaga
     4561 aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata tggcggcacg
     4621 gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt catatcacag
     4681 cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc agccagtttc
     4741 ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg gcggtaatag
     4801 agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt tccattacta
     4861 gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca aggaagttca
     4921 gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc gcgtcgagga
     4981 ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc gagcctaatg
     5041 ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg tcttcactgc
     5101 ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga tgtttcgcgg
     5161 ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga tggcttatta
     5221 tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc gctttgtcat
     5281 gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag tcaaacattt
     5341 taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg ccgcgcagga
     5401 tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc cagtgatgcg
     5461 gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg cccttttgtt
     5521 tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca acggtatgca
     5581 tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca aggatagcag
     5641 gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag accctctccc
     5701 ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg ggacgggtaa
     5761 tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga ctttcaggcg
     5821 gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg ctatccagcg
     5881 ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg agatacgcgc
     5941 catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca ggggtattcg
     6001 gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct tcccgtccgg
     6061 caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc agcggaaagc
     6121 gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc aaaatgccga
     6181 tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta ttttgctgga
     6241 tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca tattcctgca
     6301 tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg ttcagcattt
     6361 cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt gcgaacaccg
     6421 ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa acataatcag
     6481 cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata gctttgcgca
     6541 gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata tccatactga
     6601 ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta caaaggccac
     6661 cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt aaaaccacac
     6721 attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga cctcaggtca
     6781 tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg gtgaggtgtc
     6841 cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg ggggttcgaa
     6901 tccccccctc accgccagat tataagtgca tgttattgtt taacatgcgc ttttttattt
     6961 ttccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata tgccccgcga
     7021 tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat actgccagca
     7081 tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct ggttttctga
     7141 acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca tcttcatagc
     7201 tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg tttccccggt
     7261 tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg gcgagtcggt
     7321 cagcctgttg tccggtgata agatgaaatc tatggtcacc cccgtttttg caacactgat
     7381 ttttgattaa tggttggctt gcttaaatct atccggcgtc tctgtgggca aggatgcccg
     7441 cgccccgatg agttccgcac ctgataatcc taaaaatccc ctcggcttca gtgagccatt
     7501 tcatctgtta ggtttttaat caggtcgatt tgccgttcat gtcatcaata atcagtcttc
     7561 gcaaaaccag aaaggtaact tgtatcttat tgtttaaaat aaactaattg agttatatca
     7621 gcatctatat caggcctgat attctttttt ctgagtagtg attgcccagg caattcgagc
     7681 cagtttattt gccagagcgc aggtaaccac attcgaatgc ttcttgttga gttgctcccg
     7741 aacccactca gcaagttttc catgcttgcg ttcaagtcgc attatgaatg agcgggcaca
     7801 ctgaaccagt aaccggcgta aatttttatc acctcgctta ctgataccca acagagtcga
     7861 ttttcctcca gtgctatact gccggggaac aagtcctgtg gaagctgcaa aatccctgct
     7921 gcatgagaac tgtttcccgt caccaagttg cgacgatagt aagctggcag taatcggacc
     7981 aacaccagga attgtcataa tgcgctgagc ggtatcatca gcgttgattg actgacttaa
     8041 ttctgattcc agctctgcta tttgctcaac aaggtaaaag tagtgggtat gcaacctcac
     8101 caaaaggcgg ctcagatatt cggggatttc gtgttcagcc aacacgagag acaaccgttt
     8161 tattacagca tcacctatcg gcaggctgat tccaaattcc agcaagaaac cgtggatctg
     8221 gttggttgtt ttcaccttat ctctgatgag cgattctctg acccggtgta acgccctcat
     8281 ggcctgctgg gtctcagttc ttggctggac aaaacgcatt gatggtcgtg aagccgcttc
     8341 gcatatggct tctgcatcaa caaaatcatt cttattactt ttgacaaacg ggcgaacgaa
     8401 ctgtggagaa atcagcttag tttcatgccc aaaatcagag atacagcgag ccataaagtg
     8461 tgcacccgca caggcttcca ttacaacaac tgagggcgga catgtcgcca gaaactgcat
     8521 cagtttagta cgggtaaatt ttttacgcag aagagcctta ccagacttgt cttgacaatg
     8581 aatgtggaag gagtgctttc cgagatcgat accaataagt gtgatgttct gcatgatggt
     8641 ctctccgtta aagggattta ccttaacagc gtactgctgt taaggtgggg gtgaccatat
     8701 cattatcccc accggtccca tctcaccaga ttgttgacga taattttact gccaaattta
     8761 caggggcaat tggttttgta attccagttc agccctctga agacgttgat cacccctcgc
     8821 tcaaacacct catccttatt ctggtgcagt tgttcaaatg tgctcagaat gttcgcttcg
     8881 ctgatttccg gaaaattatc gtactccagt gaccggtacc atgtatcacg ggcctgcgta
     8941 tccattaatg acagcatccc ggaacgttgc atcaggtcac gccagatttc gcgatcaaga
     9001 ttccgggtaa tggctttcat tgctgtttcg ggtttctcca tcagccagca gccgcagcga
     9061 aaatcctgtt tcatggccca gtccggggca gttttacctc cgatgctttc cgtcagcacc
     9121 gagatttcag ccagttgtcg gataagtgtg tcgatttgca acagtgtact ctttcgtaca
     9181 gccagaatgt ggcgaatgct ggttgagcag ataacgtccg tatgcccgtt gaccacttcc
     9241 ggacgggaga ttgtgatatc tgacataatc gatattcctt tttgcagata aatgaaaacg
     9301 ccagccctgt gcaggctggc gtcagaggaa tgaagtctgg tgagtgagct tcaccggttc
     9361 tggtgcagaa gctgcagatg acgcagggta cggacatcct cccgggcctg ttcgatacag
     9421 ggcaacagtg ctgctgaatc cgttttatcc tcatcgttgt caaagataat tcccgatccg
     9481 cagtcgatat tgtcctgcag ccacgcaatc agaatatcca gcgctgtttc cgtggttaat
     9541 gatttcatgt tgttcctggt taccagtcga aagtgggtaa acccggcagg cattcagcgc
     9601 tggcatccag atgaatgaga ctgacgccat aacgccggat gagtgtgacg accagacgac
     9661 ggaacgtttt tgacagcccc aggcgtttaa gccgcagaac cgggtatgac cacgcatcgg
     9721 tacgtaacag atatccgcta cctgtaaaat gaatccattc tgattcaccg aagtcactgg
     9781 tctggtgtga cagcgagtac aaccaggcgt tgtccgtttc cgtgatatgt gcggtactgc
     9841 agcgtatgcc ggtaagggta gcaaatggtg gcgtgcattc tgctgaggtc tggtcagatt
     9901 catcaacaac acgaagggag tagccgttat cgcagaccat gttaatcagt tcagcgagct
     9961 caataccatc gacgtcaacg acaatgcgcc ctatgttcag tgcctgtacg ttaacgctgt
    10021 cggcttccag tgtcagggcc agtttcatca tttcgcctcc ggatacttac ccagggtaat
    10081 gttatttacc gttctgtaat tgtcgcgggt catcaggccg gtcgcccggc gagcccggag
    10141 gatatcaata ctgtttatta actgagagcg gggacaggtg ctgaatcccg gctggtcggt
    10201 acgcaccagc gcgtattttt ccacgagaaa attaactgca tcacacagtg aaatgcctgc
    10261 ctcaatatgc tgctcaatca cacgttcatc gacaaacggt gtgtcattca gtgtgaggcc
    10321 ataatgctgg tccagcagtc ggatgagcag tgtctgccag atggtgacgg gagacgggca
    10381 gcgagatacc tcccgtacat gtgtgtcgga taatgttttc atcttaagtt ctctgtaact
    10441 ggtagtgcta aaggtgagag cttcggggtt atgaggtggt tgcgggtgct ggtgtcggat
    10501 aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt
    10561 ttgcatacag cgtgacggta tgctgatatc ggggattcag ttcaccgctg gtgagcatga
    10621 gttccagttg tttcatcagc agcggaaagg cctggtccag atggtatgca tctgcgtcgc
    10681 tgaagcggcc tctgataccg gcgcggtcgg caaggtaata caggcggtta ccctcttgca
    10741 ccagacgagc gccaaaacag ggcctcactg tgcagggaag cccccaccag gggcggtcgt
    10801 gattgtcatc gggatgagtt atcccggaga gtttgtctga cacgataaaa tcctctctga
    10861 aaagtggtta agaatggttt aacgatgaca ggtcagccgg cgcgctgacc ggtttcggtg
    10921 aagatgtatg cattgatgag aatggcctca tccacagcgg cgtctgaggt catctggtcg
    10981 tactcgtttc ccgggtgacg gaacatcagt tcagggtgac actcatcagc cggacatacg
    11041 ggccaaaact gtccttacgg cgttctgcaa acacggcaag aacgccggga atatcctgca
    11101 cttcacgacc agtatacgct tcagcactgc cgtgccagcg atacttaccg gtacagaacg
    11161 gaaaaagacg ggatgcagga tgctgttggt gaatacgcat ggcttcacca cgggtgatga
    11221 ttctcatgat gtgatacctc tgaagacaga agataaaagt gaaaacaggt gtgatgtggt
    11281 tgtgacgggt taaagcagac catgttcggc aaaggagaaa acctggttgc caccgactat
    11341 cagatggtcc ggcacccgga tatccaccag gcccagtgcc tgtaccagac gttcggtgat
    11401 aagccggtct gccttactgg gtgtgacttc accggacggg tgattgtgcg ccagcaccac
    11461 ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc cggggatgaa cttccgtacg
    11521 gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc tgattctggt tgttcagata
    11581 cagcacccgg aactcttccc gctccagtcc cgccatgttc agaatcagcc attcccgtgc
    11641 cgcacgggtg gaggtgaagg ccacgccggg ttcatgaaga tggcggtcca gggtttgcag
    11701 ggcccgcaga atgagactgc gctcgccggg cgtcatgttt ccgggcagaa aggaaagctg
    11761 ctgcattgtc cctgcctcca ttcagtcgat gatgcgcata atggcgctgc attccggatg
    11821 ctgcagggcg taatcccgca gccggtaata atggaccgtc atggcataac actccgtacg
    11881 acaggcatga tgactgtacg tcatcagaca ggcagcaatg ccggcggctt ccgggctcat
    11941 ttcagcgcgg ttaccgttca tggcattgaa cagtacccat gtttcgtcat catcgtcatc
    12001 cggttcgggt gccataaatg ccccgccgtt gttcagggtg tacagattcc agataccacc
    12061 gcagtagtct tcacacagac ggtccatcca gccgaagaca cggggttcca gggtgaccca
    12121 ctgtggaatg aggccaaaat gctgcggcca gaagctgatg cgctgttcat cagggacggg
    12181 agtggcaacc agttgttgca ggccagtctc cggtgcacag gccgaagcgg tggtgttctg
    12241 agacaatgtt ttcatgtatg aaatccttgt aatgtgaata aatgagaggg agtaaaggtc
    12301 gggagctggc aacatcacgt tcatcactga ccgtgatgac ccggtaatat cagggatgaa
    12361 tgatattcgg ctgaaattcc ggacagccgt acgcgtaacc gttccggtca cagacggatg
    12421 gccagatgcc attgcctgta tactcagtct gcacagactg atttctccgg gacggtaaag
    12481 cagacgggta caccaggcgc acaggtcatc atcagaaatg gaacgacacc ccggcagcgc
    12541 ctccggggcg tcgggtgatt ctggtgcctg aatcacgcct tcagggacat gctgcctcag
    12601 cgcaggctct ccagcagcgt ttctgccatt acccacaatg cgcggttgag cttaatatcg
    12661 gtgtcgatgt tgtgaatggc acgggtgtgg atacgttttc cttttgcact gcggccggaa
    12721 atcccgcctt tcagcatatt ctcctggatg gtctgatatg cgctccacag gtctttaccg
    12781 taatcctccc tgcgtcgtgg tgtcagaatg tcagcggtgg tgactggctg atgttcgtca
    12841 ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcaggacc
    12901 agcgactgca tggcatcacg cttctcctct atccggtcaa acacgcccac cacctcgtaa
    12961 gccccttcaa taactttctc caccacattt ccccggtgtg gaacacgcaa ttctcccaga
    13021 gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg taacatctgg
    13081 tagctggatg aaccgtcatg agagttgagc agaataattt cagggacatg ctgaccggtt
    13141 atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg acccgggtca
    13201 cgcacacgtg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag gctttccagt
    13261 acggtgatgg tgggagtgta cgcatagcgt ttactgcggg aagtatgccg gtcttcaccg
    13321 aaaatactgg gaacatagtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc
    13381 tggtttgcag cataaccaaa acgactggct agtcgcataa tttgctcctt atcggtggtt
    13441 aagatttact ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg cggcctgatg
    13501 gacgaaatta atgaggttta ttgtctgaga agaccgtcac tggcgaacct tccggcgtca
    13561 gtctctgcgt tgcggataac ccgacggaaa tgagttccct ggtcatcgtc gataaacaca
    13621 ttgcggtaag ctgttgtgat gacaacagcc tgctcgcgtg agaaagggac ttcgggcaat
    13681 aaacgtgaca tacaaccaga cctgtatttg gttccctgaa aatataagtc cctgtcatca
    13741 tggcagagcc gtatttactt ttgatatcaa taaattaata acgcgaagat gaaccgcata
    13801 acttacgcct ttaatttgtt ttgtttacgt agtccctgac caggtacgcg aattcgtgaa
    13861 cgccagtttc accaaaccaa atcaggtaac cagggtcgat gttgtaatag taaccaacga
    13921 ctttatgcgt ttttgggtta tagagtgtta cgatatagtc ccagccgcag gcgtgggctt
    13981 tacaaccgcc accttctacc caggtcgttc cgttaatgtt tacaggatac gcaccaccat
    14041 caggaccatc catatagccg tcttccccaa acattccgtt taacccaaca gggtttaatg
    14101 ctttgtcgat agcagctctg taggtagcgt caccttcgta taactcgtac gttgttggac
    14161 atgcaccagg ctggtcagcc cgggcttttg agtcgcattt aagtgtctga tttacagtgg
    14221 cggcgtttgt gtcaggactt
//
LOCUS       DAJTNW010000097        13963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNW010000097 DAJTNW010000000
VERSION     DAJTNW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="OO179_003268"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="OO179_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5533228.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="OO179_003269"
     CDS             2218..2766
                     /locus_tag="OO179_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5533229.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="OO179_003270"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="OO179_003270"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5533230.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO179_003271"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO179_003271"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5533231.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO179_003272"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO179_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5533232.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO179_003273"
     CDS             complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO179_003273"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5533233.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO179_003274"
     CDS             complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO179_003274"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5533234.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9764..12379
                     /gene="pepN"
                     /locus_tag="OO179_003275"
     CDS             9764..12379
                     /gene="pepN"
                     /locus_tag="OO179_003275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5533235.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO179_003276"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO179_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5533236.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..>13963)
                     /gene="ssuC"
                     /locus_tag="OO179_003277"
     CDS             complement(13350..>13963)
                     /gene="ssuC"
                     /locus_tag="OO179_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5533237.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 aacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgctggt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgtgacg tggtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccgatcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcgctttc aacgccgccg ctttcggtga ttaaccaatg gcagctagcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg tgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggagcagat
     1201 ggtgctattg gcccggcaac gcgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggcac tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccagccta ttcgctgctc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcacttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta ccaccaactc tggcacgcaa agatattctg
     1501 ccgaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg gaggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt ttcgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggatgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tgatcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcaa ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgag ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttccattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attacccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat cgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttgatctggt gtgaacaaac ccgcctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatct acacttggtt atgagcgcct gcacaatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa aggcgcataa aaaagtccac ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaac
     3661 gcttacagca ctgccacaat cgattcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaacagca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcagacc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcaccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaatgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggagggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acgatttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcgttcagac tgttaatcag tgcatcgaag
     4381 tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg cttaacgctg
     4501 gtattttttg ctaggaagtc ggcagccacg cgtagtgcgc cagtgccacc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcaag gatgccgtca atgctgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggc
     4981 ataaaaaaaa caggaccaac gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt tatgacacct gccactgccg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggagctttg tgaacaccgt
     6361 catatttcca tagaaacgtg atgatattta cagcaatttt tatctattta tatgatttac
     6421 ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat cgacctggca
     6481 caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg tgctatcagc
     6541 taagaaaaaa ctaatccgca ataagaatgc gggttatttt ttgtgcgtaa atgttgcaat
     6601 gtaatattcc gtaacaggat gatcgttatg gcgggggtgc aaatagattg cttgacttaa
     6661 caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc tctttgagga
     6721 agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat cacgcacgtt
     6781 ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac ctgaatgcgg
     6841 aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat tcaggcccat
     6901 ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc caccaatgat
     6961 ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt cttcgttgag
     7021 gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg gtgctttaaa
     7081 gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc agtaaaccgg
     7141 gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt aatccacctg
     7201 cgcgaaatcg gcttcaatga agcgttccag acgtgaaacg gcatctttat ctacgcgttc
     7261 agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga catatttcag
     7321 catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt ccggctccag
     7381 catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga aagtcgggcc
     7441 gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact ggccagatac
     7501 ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt
     7561 acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg caccttcggt
     7621 atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg
     7681 atgcagcgcc tgcgccagcg tatggcgaac gcgcgcgacg gcaccaatca ggtttgtgcg
     7741 cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg ccgccatcgg
     7801 gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct gaatttcaaa
     7861 ttgttgcccc tggcccggcg atgccacgac tttacccgtc acaatgaccg agcagccggt
     7921 ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga cagcctgtac
     7981 aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt ttgaatctcg
     8041 gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc ggccctggag
     8101 tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg gaaaaaataa
     8161 acacttgtcc acccgtaaat gggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaag ggagccgatt
     8281 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
     8341 cgcccgaaca aacgctttat catggcagat agttttgcca gggctgtcag aaagtttcgc
     8401 caccggttta ccgttacact ctaccaactt aatcacaata ttcaggggtt ttacctgggg
     8461 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
     8521 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gcgttttact
     8581 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
     8641 gtcgccagag tcatgacgca ggccctgata ccgactagcg aactcgacac cgaaatcacg
     8701 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatactc
     8761 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
     8821 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
     8881 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
     8941 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccatcagat ggaagcgcga
     9001 catatcaaga ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
     9061 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
     9121 cagcaaagga acttcccaga ggatgacttc acgccacgga ccgcttaagc gaatatccag
     9181 cttgccatta tcgttggaca cggtgacttg ttccgggtta aagcggaact cgcgtaacca
     9241 gttaagatag tcggccttaa agaaaggcag ggcagagagc cactgatatt catcatcctg
     9301 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
     9361 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
     9421 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
     9481 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
     9541 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
     9601 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
     9661 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
     9721 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
     9781 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
     9841 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
     9901 tgcatcagat gctccgcttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
     9961 taatgatgag ccgtggaccg cctggaaaga agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggtaa agtgatcccg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tagacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgtcgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatccgg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccagact
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctgccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggt gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gct
//
LOCUS       DAJTNW010000098        13958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNW010000098 DAJTNW010000000
VERSION     DAJTNW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            295..1548
                     /gene="kch"
                     /locus_tag="OO179_003278"
     CDS             295..1548
                     /gene="kch"
                     /locus_tag="OO179_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5533238.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1603..1776)
                     /locus_tag="OO179_003279"
     CDS             complement(1603..1776)
                     /locus_tag="OO179_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5533239.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1919..3379
                     /gene="cls"
                     /locus_tag="OO179_003280"
     CDS             1919..3379
                     /gene="cls"
                     /locus_tag="OO179_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5533240.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3414..3743
                     /locus_tag="OO179_003281"
     CDS             3414..3743
                     /locus_tag="OO179_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5533241.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="OO179_003282"
     CDS             complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="OO179_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5533242.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="OO179_003283"
     CDS             complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="OO179_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5533243.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="OO179_003284"
     CDS             complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="OO179_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5533244.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="OO179_003285"
     CDS             complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="OO179_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5533245.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="OO179_003286"
     CDS             complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="OO179_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5533246.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="OO179_003287"
     CDS             complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="OO179_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5533247.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11244..13919
                     /gene="adhE"
                     /locus_tag="OO179_003288"
                     /gene_synonym="adhC"
     CDS             11244..13919
                     /gene="adhE"
                     /locus_tag="OO179_003288"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5533248.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3660 a         3454 c         3246 g         3598 t
ORIGIN      
        1 gatccaatat ttaagggcac ggtttttgca accgtgttct tgtttggcct ttccttatag
       61 ccggtcagtg cattcactac cacaagcaga taataacttt atctgtattt ctttatatga
      121 tgtgagcatg ttcaggaaaa aagccctttc ctgacgcaga gttgctcact caaaaccttt
      181 gattactgcg gataaagtca tctactattg tttaacacat tcacgcgctg catgtatgtt
      241 cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt atcagtgagt
      301 cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg gcacgacatt
      361 ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta tggtatgtcg
      421 gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct ctccttgctg
      481 gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat tggcttactg
      541 ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc gctaatttac
      601 accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt tacgctggtg
      661 tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg gacaattttt
      721 gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct ttatttaagc
      781 gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc gatagaaacc
      841 atgtcaaccg tcggctatgg agatattgtc cctgtttctg aatcagcacg attgttcact
      901 atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc aatttttggt
      961 ccgcttatcc gcgggggatt caacaaactt ttaaaaggaa acaatcatac aatgcatcgt
     1021 aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat cctgcaactg
     1081 aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga tatcaagcaa
     1141 cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa tgacagttca
     1201 gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag tgataacgat
     1261 gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt caaaacagtt
     1321 ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc ggatatcatt
     1381 ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg tgaagagatt
     1441 aataatgata tgctcgtttc aatgttgttg aactcaggtc atggtatttt cagcgataac
     1501 gatgaacaag aaacgaaagc tgacagtaaa gaatcggcgc aaaaatagag tttaaagacg
     1561 gaagagtaaa cttattgggc accgcaatgg tgcccagtac atttagatat tgtagatcgc
     1621 aaacaataac gagttacgct gtttgtttag aatgcacttc ctgatgctgt ggatacgcat
     1681 atttcggcgc gattgcccct caagccaacg cgatttacgt cggctagcct gacgctgcat
     1741 gcgccagcgc cccacttccg ttctactccg cttcatgtta actactctat gcaataacag
     1801 aatggtcatt atacccccgc ccccgacgct gaccagtgct tttcccgcgt ttttatttgc
     1861 cagatgaatc catatgcgta aactcataac aatgcgcttt caaaaggatt tctaatctat
     1921 gacaaccgtt tatacgttgg tgagttggtt ggccattctg ggatactggt tgctcattgc
     1981 aggcgtaact ttacgcattc taatgaaacg acgcgcagtt ccctccgcga tggcctggct
     2041 gttgattatt tacattctgc cgttagtcgg aattattgcc tatcttgccg ttggcgagct
     2101 ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg tggccttcca ccgcaaaatg
     2161 gcttaacgac cttaaagcct gtaagcatat cttcgccgaa gaaaatagca gtgtcgctgc
     2221 gccattattc aagctttgcg agcgtcgtca ggggatcgct ggggtcaaag ggaatcagct
     2281 acagctgatg accgagtcag atgatgtgat gcaggcgtta atccgcgaca tccagctcgc
     2341 gcgccataat attgagatgg tgttttatat ctggcagccc ggcggcatgg cggaccaggt
     2401 ggctgaatca ttaatggcgg ctgcacggcg tggtattcat tgccgattga tgctcgactc
     2461 cgccgggagt gtggcttttt tccgcagccc gtggcccgag ctaatgcgta atgccggtat
     2521 tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg tttttacgcc gtatggacct
     2581 gcgccaacat cgcaagatga tcatgatcga taattacatc gcgtacaccg gcagcatgaa
     2641 tatggtcgat cctcgctact tcaaacaaga tgcgggcgta gggcaatgga ttgatctgat
     2701 ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt atttattcct gcgactggga
     2761 gattgaaacc ggaaaacgta ttctgccgcc accaccagat gtcaatatta tgccgtttga
     2821 acaggccagc ggtcacacca ttcacacaat tgcttctggc cccggctttc cggaagatct
     2881 cattcaccag gcattattga ctgcggctta ttcggcgcat gaatatttga tcatgaccac
     2941 gccctacttt gtgccaagcg atgatttact tcatgcgatt tgcacggcgg cgcagcgcgg
     3001 ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg atgctggtcg gctgggccag
     3061 tcgcgcattc tttacggaac tgcttgctgc tggggttaaa atttatcagt ttgaaggcgg
     3121 gttactgcat accaagagcg tgctggtcga tggcgaacta agtctggttg gcaccgttaa
     3181 ccttgatatg cgtagtctgt ggctgaattt cgagattacc ttggcaatcg acgataaagg
     3241 ttttggtgca gaccttgccg ccgttcagga cgattatatt tcgcgttcac gtctgctcga
     3301 tgcccgttta tggctaaaac gtccattatg gcaacgtgtc gccgagcgac tgttttactt
     3361 cttcagcccg ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg actatggata
     3421 tggatctaaa caatcgcctg actgaagatg aaacgcttga gcaggcctac gatatttttc
     3481 tcgaactggc tgccgacaat ctcgatccag ctgatgttct gctgttcaat cttcagtttg
     3541 aagagcgcgg cggcgcggag ttattcgatc cggcggaaga ctggcaggaa catgttgatt
     3601 tcgatttgaa cccggacttt tttgccgaag tggtgattgg tctggcggac agtgaagatg
     3661 gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg cgaaaaagat cacaaacttt
     3721 gccatatcat ctggcgggaa taaataatcg cttttagcgt gaaaaagggc tgacaactgt
     3781 cagcccttat tgtttttaaa gcggatcgac tttcaggcaa gaaacggcgt gtctgaaact
     3841 cccctccaga acaggacgtg ttttggcgca ctccggaccg gcaatcgggc aacgggtacg
     3901 gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat tccccttcca gtaactggat
     3961 ggttttgttc ttctccagat ccggatcagg tatggggact gccgacatca atgccctggt
     4021 gtaaggatgt agtggattgt ggtagacctc atcataggtc cccagttcta ctgcatggcc
     4081 gagatacatc accaacacac gatcggaaat gtgttttacc acggccaagt catgagcaat
     4141 aaaaattaat gacaatccca tctcacgttg cagctgctgg agcaggttaa ccacctgcgc
     4201 ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag ataatcagct tcggttcaag
     4261 aataagagca cgagcaatcc cgatacgctg gcactgccca ccggagaact catgcggata
     4321 gcggttaatc aggttaggca ataacccgac tttcagcatc atcgccttca cgcgctcgcg
     4381 aacttcctgg cgtgacattt tcggatgata agtacgcagt ggttcagcga tgatctcgcc
     4441 gatggtcata cgcgggttta gcgatgccaa cggatcctgg aaaatcatct gaatatcact
     4501 gcgaacggca cgccattcat cgggcttcat gcccagcaac tctttaccta accaggcaac
     4561 atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga gcaaaggtgg acttaccgca
     4621 tcccgattcc cctaccacac ctaatgtttc cccttcatac aggcgaagag tgacaccatc
     4681 gacggctttg agcgttttcg gcggttgcca gaaccactgt ttgccatctt tgatttcaaa
     4741 gtgcacttta agatcggcga tttcaaggag gacttttctt ccttcagtta cagcattcat
     4801 aacagttcct ccaccggttt aaagcaagca cgcagacggc caggcgtaaa ctcttccagc
     4861 ggcggtgcgc tactacaaat ttccatcgca tgtggacaac gtggctggaa cgggcaacct
     4921 ttcggtaatc gcagcaagtt tggcggatta cccgggatgg tcaacattgt ttcaccttcc
     4981 gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat aaggatgaac gggttgataa
     5041 aagacatcgc gagcattgcc atattccatc gtgcgcccgg cgtacattac cagcactttg
     5101 tcacagatcc ccgccaccac gccaagatcg tgggtgatca taatgatggc agtattaaat
     5161 tcccgcttca gttcattcaa tagcgtcatg atctgcgcct gtacggtgac gtccagcgct
     5221 gtagtgggtt catccgcaat cagcaactta ggtcgacata gcaatgccat cgcaatcatg
     5281 actcgctgac gcatgccgcc agaaaattcg tgcgggtaca ttttcatgcg tttacgtgct
     5341 tccggcattt ttaccgcatc gagcatccgc accgactctt caaacgcctc agctttgctc
     5401 atgttcttat gcagcatcag cacttccatc aactgctcac cgacgcgcat atagggattc
     5461 aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt cagcgcgcag tttattgagt
     5521 tcacgctctg gcaaattgag gatttcacgc ccattgaagg ttgccgatcc gccaatgcgt
     5581 ccgttggcag ccaacaggcc catcaacgca aatgcagttt gcgatttacc cgaaccagac
     5641 tcacctacaa tgcccagcgt ttctccggca cgtagggaaa aatttaaatc attgaccgcc
     5701 gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat ctttcacgtt cagcagtgcg
     5761 tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa ttacgctcat ggctgcactc
     5821 cttaacgatc tttcgggtcg agggcatcac gcaagccatc gccgataaag ttgaaacaaa
     5881 acagtgtcac cacaaggaat cccgctggga acaacagtaa ccatggagag acttccatcg
     5941 agttcgcgcc atcactcagc aatgcccccc agctgcttaa cggctcttgc gtacccaacc
     6001 ccaggaagct aaggaaagat tcaaagagga tcatgctggg caccagtagc gatgcgtaga
     6061 ccaccaccac accgagtacg ttcggcacaa tgtggcgaat aacaatgccc ggcgtcgata
     6121 caccgccaac ttgtgccgcc tcaataaact ctttgcgctt cagactcagg gtttgcccac
     6181 gcacaatacg agccatatcc agccaggaaa ccatgccaat cgccacgaaa atcagcagga
     6241 tgttttggcc gaaaaaggtc accagcaaaa tgacgaagaa catgaatggg aaggagttga
     6301 ggatttccag cagacgcatc attaccgaat ccactttacc gcccagataa ccggaaagcg
     6361 aaccataaag tgtccccacg accactgcca ccagtgccgc agcaacaccg accatgagtg
     6421 agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg accggatgag tcagtaccaa
     6481 agtagtgacc ggactccata tccggggcgc tggacatcat cgcccagtca gtatcgtcat
     6541 aggcaaactg ggaaagcatc ggtgccagga ttacaaataa cgcgatcagc accagcacta
     6601 tcagactggc aaccgccgca cggttatgca taaaacgtcg acgtgcgtcc tgccacaagc
     6661 tgcgcccttc gacctccagc ttttcactga aattttccag cgtctcgctg tttttcttac
     6721 ttaacatcat cgcgagctcc agtatcagta acggattttc gggtcgataa ccgcatatag
     6781 cacatcgaca atggcattaa acaaaatggt taacgcacca accaggatgg tcaggcttaa
     6841 cactaaggaa tagtcacggt tcaatgcgcc attaacgaac agttgcccaa tccccggcaa
     6901 accataaatg gtttcgatga ccatagaacc ggtaataatg ccgacaaatg cggggcccat
     6961 ataggagagc acgggtaaca gagcaggttt taatgcgtgg cgtaaaatga tccgccgcat
     7021 aggtaaccct ttcgcccggg cagtacgaat aaagttggag tgtaatactt caatcataga
     7081 gccacgggta atacgcgcaa tactggcgat ataggcgagt gacaacgcca ccatcggcaa
     7141 tatcatgaat ttaagcgccc caccattcca gccaccgccc ggcagccaat gcaaaatgat
     7201 cgcaaatatc atgactaata atggcgcaac cacaaaactg gggataacaa ccccggtcat
     7261 tgccagcccc atcacggtat agtcccattt ggtgttttgt tttaatgcgg caataacgcc
     7321 agcactaaca cccagtatta ccgcaaggaa aaatgcggcg gctcccagtt tggcagaaac
     7381 gggaaaactg gatgccacca ggtcattgac cgaataatct ttatatttaa acgatggacc
     7441 gaaatcgcca tgcgccagtt gtttcaggta gctgaaatac tgtgtcatga tcggatcatt
     7501 aagatgatat ttcgcttcga tattggccat cacttctggc ggtaaagtac gttcgccggt
     7561 aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa atagtaataa gaataaatag
     7621 cgtcggaatc gcttccagac aacgacgtag aataaatttt aacattgccc taccttctgg
     7681 cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg agcaggacac tcctgcccca
     7741 cgtattgcca ttagtgcttc acaatgtaca tattccgggt ataggtatta tccagcggat
     7801 ctttgccggt atagccacca acccacggtt tcaccagacg cgcattcacg tagtaataaa
     7861 caggaacaat ggccgaatcc ttatccagct gttgttctgc tttagtgtac agagctgtgc
     7921 gctgcgcctc gtcagtcact ttcagcgttt ccgccataat gctgtcaaag gccgggctct
     7981 tataatgcgc ggtattcatc gagctgttcg acagcatggt gttcaggaag gaagttggtt
     8041 cgttgtagtc tgcacaccag cctgcacggg ccacatcaaa agtgccctgg tgacgggtgt
     8101 cgaggaacgt tttccactcc tggttaacca gtttgacgtt taccccaatg tttttcttcc
     8161 acaatgaaga ggcagcaatc gccagctttt tatgcagatc ggaggtgtta taccgcaggt
     8221 tgatggtcaa cggtttgtct gcggtataac ccgcttcagc cagcagtttt ttcgcttctt
     8281 cgttacgttt ttcctggctc cagccaaacc attctggctg agtcaatttt gcgccatcag
     8341 tatacggtgg agtataacca taggcgggca tgtcgccctg cgctttcact ttattaacaa
     8401 tgatatcgcg gtccatacct agtttcagtg cggtacgcac acgcacatcg ttgaatggcg
     8461 gtttctggtt gttaatttcg taatagtaag tgcacaggta tggatcaacg tgaacttcgt
     8521 ccgggatctc ttttttcagc ttctggaaca attcgatcgg catgttgtta tacgtcatgt
     8581 cgatttcacc actacggtag cggttgacat cggtaacttc agaagcaata ggcaaatagg
     8641 ttacctggtt aataacggtt ttcgcgttgt tccagtaggt cgggctgcgt tcaagaacga
     8701 ttcgttcgtt tacgacccaa tcttttaagg tataggcacc gttggtgacg atattaccag
     8761 gctgggtcca tttttcgccg aatttttcga tagcggcttt tggcaccggt gaagttgatg
     8821 ggtgaacaag taatttatag aagtacggaa cgggttcact taaggtgact tctaatgtgt
     8881 gatcatcaat agctttcacg ccgagatcgg taatcggttt tttcccttca agaatttcat
     8941 caataccggc gatatgccca tattgcagat aactggcata cggagaagca gtgttcggat
     9001 caacagaacg ttgccagcta tacacaaagt cttgtgctgt gactggcgtg ccatcagacc
     9061 atttcgcatc tttacgcaaa tggaaggtcc agactttcgc gtctttatta tcccaggatt
     9121 cagcgacgcc aggtgctgga tgaccgtcaa gatcgctgac cagtaagcct tcaaacaggt
     9181 ctcggctgat attagactcc ggaacacctt caattttgtg cggatctaat gactgaactt
     9241 ctgaaccatt gttacgtacc agtgtttgtt tttccgccag tgtgacgcct gcgggtacat
     9301 cagctgccag cgcgacattc cctgccatta gcgcagccag aacgccagct gctactaaac
     9361 ttctcttggt gatgttggtc attgtttttt ggactccctc attataatta ctggtattaa
     9421 ccagcatgtg taatcccgga ttgggaccct gcacaagctc ggagactttc tgctgtcagg
     9481 ttactttatc gttacgtatt actatcaccg actttattta ttctggtgat tcagacgatc
     9541 accttatgtc gattcgtact gtgtctcccc tgtcgagaca cattattctt tcgcgacgct
     9601 ctttaattgt taattattct cattaccaat aaagaatcgt ctggcggttg cccggaaagt
     9661 accaaatgcg gttcttactc gccaatacat tttgcaaaat tgttaagcga ttctctttta
     9721 ttgaccgtat cagaatctat agcttgcatc catgccttaa caaacatctt tcaattcaat
     9781 caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt agtgaaatgc
     9841 acaataattt aacaattgat tattatttag cactttcctc taccaaaggc agtggaatta
     9901 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
     9961 gatggtgagg agaaacagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
    10021 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
    10081 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
    10141 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
    10201 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
    10261 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
    10321 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
    10381 cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga
    10441 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
    10501 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
    10561 tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
    10621 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
    10681 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
    10741 gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta
    10801 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
    10861 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
    10921 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
    10981 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
    11041 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
    11101 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta
    11161 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
    11221 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
    11281 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
    11341 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
    11401 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
    11461 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
    11521 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
    11581 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
    11641 ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg
    11701 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
    11761 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
    11821 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
    11881 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
    11941 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
    12001 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
    12061 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga
    12121 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
    12181 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
    12241 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
    12301 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
    12361 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc
    12421 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
    12481 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
    12541 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
    12601 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
    12661 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
    12721 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
    12781 tcttcgaagt agaagcagac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
    12841 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
    12901 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
    12961 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
    13021 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
    13081 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
    13141 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
    13201 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
    13261 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
    13321 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc gcgggtatcg
    13381 catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
    13441 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
    13501 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
    13561 gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg
    13621 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga
    13681 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
    13741 ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg
    13801 agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
    13861 cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
    13921 cagtagcgct gtctggcaat ataaacggcc ccttctgg
//
LOCUS       DAJTNW010000099        13956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNW010000099 DAJTNW010000000
VERSION     DAJTNW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO179_003289"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO179_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5533249.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO179_003290"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO179_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5533250.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO179_003291"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO179_003291"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5533251.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO179_003292"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO179_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5533252.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO179_003293"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO179_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5533253.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO179_003294"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO179_003294"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5533254.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO179_003295"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO179_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5533255.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO179_003296"
     CDS             7490..7639
                     /locus_tag="OO179_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533256.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO179_003297"
     CDS             7840..9423
                     /locus_tag="OO179_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5533257.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO179_003298"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO179_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5533258.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO179_003299"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO179_003299"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5533259.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO179_003300"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO179_003300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5533260.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13801
                     /locus_tag="OO179_003301"
                     /pseudo
     CDS             13382..13801
                     /locus_tag="OO179_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(13826..>13956)
                     /locus_tag="OO179_003302"
     CDS             complement(13826..>13956)
                     /locus_tag="OO179_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5533261.1"
                     /translation="YYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         3227 a         3680 c         3537 g         3512 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagtat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacaaacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgttgggggg
    13801 tgcccctatt aagttgtcaa gcatgttatg acccctgcgg ggtataaaaa gtcccgtcgc
    13861 gcatcatggc gaacagaacg tcgcagcgtc gtctcgccag ggcgataagc acctgattgt
    13921 gtcgttttcc ctgactcatt ttgcgggtgt agtaag
//
LOCUS       DAJTNW010000100        13533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNW010000100 DAJTNW010000000
VERSION     DAJTNW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..890)
                     /locus_tag="OO179_003303"
     CDS             complement(<1..890)
                     /locus_tag="OO179_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5533262.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO179_003304"
     CDS             1131..1931
                     /locus_tag="OO179_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5533263.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO179_003305"
     CDS             2009..2779
                     /locus_tag="OO179_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5533264.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="OO179_003306"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="OO179_003306"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5533265.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="OO179_003307"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="OO179_003307"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5533266.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="OO179_003308"
     CDS             5046..5768
                     /locus_tag="OO179_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5533267.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="OO179_003309"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="OO179_003309"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5533268.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="OO179_003310"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="OO179_003310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5533269.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="OO179_003311"
     tRNA            7161..7237
                     /locus_tag="OO179_003311"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="OO179_003312"
     CDS             complement(7368..8414)
                     /locus_tag="OO179_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5533270.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="OO179_003313"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="OO179_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="OO179_003314"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="OO179_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5533271.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="OO179_003315"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="OO179_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5533272.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="OO179_003316"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="OO179_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="OO179_003317"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="OO179_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3522 c         3611 g         3299 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tgt
//
LOCUS       DAJTNW010000101        12995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNW010000101 DAJTNW010000000
VERSION     DAJTNW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..309)
                     /locus_tag="OO179_003318"
     CDS             complement(<1..309)
                     /locus_tag="OO179_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5533273.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     Y"
     gene            complement(515..1234)
                     /locus_tag="OO179_003319"
                     /pseudo
     CDS             complement(515..1234)
                     /locus_tag="OO179_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="OO179_003320"
     CDS             complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="OO179_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5533274.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="OO179_003321"
                     /pseudo
     CDS             complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="OO179_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3145..3828
                     /gene="cusR"
                     /locus_tag="OO179_003322"
     CDS             3145..3828
                     /gene="cusR"
                     /locus_tag="OO179_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5533275.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3818..5260
                     /gene="cusS"
                     /locus_tag="OO179_003323"
     CDS             3818..5260
                     /gene="cusS"
                     /locus_tag="OO179_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5533276.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5410..7647
                     /gene="nrfB"
                     /locus_tag="OO179_003324"
                     /pseudo
     CDS             5410..7647
                     /gene="nrfB"
                     /locus_tag="OO179_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7634..10602
                     /gene="nfrA"
                     /locus_tag="OO179_003325"
                     /pseudo
     CDS             7634..10602
                     /gene="nfrA"
                     /locus_tag="OO179_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10603..11493
                     /locus_tag="OO179_003326"
     CDS             10603..11493
                     /locus_tag="OO179_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5533277.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11676..12437
                     /gene="envY"
                     /locus_tag="OO179_003327"
     CDS             11676..12437
                     /gene="envY"
                     /locus_tag="OO179_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5533278.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12483..12559)
                     /locus_tag="OO179_003328"
     tRNA            complement(12483..12559)
                     /locus_tag="OO179_003328"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12523..12525),aa:Arg,seq:tct)
     gene            12802..>12995
                     /locus_tag="OO179_003329"
     CDS             12802..>12995
                     /locus_tag="OO179_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5533279.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDG"
BASE COUNT         3179 a         3366 c         3304 g         3146 t
ORIGIN      
        1 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
       61 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      121 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      181 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      241 agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      301 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      361 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      421 caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      481 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      541 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      601 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      661 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      721 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      781 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      841 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
      901 ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
      961 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1021 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1081 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1141 tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1201 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1261 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1321 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1381 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1441 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1501 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1561 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1621 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1681 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1741 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1801 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1861 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     1921 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     1981 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2041 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2101 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2161 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2221 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2281 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2341 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2401 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2461 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2521 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2581 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2641 gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg ggtagcggtc
     2701 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2761 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2821 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     2881 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     2941 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3001 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3061 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3121 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3181 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3241 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3301 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3361 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3421 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3481 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3541 ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat cactctgacc
     3601 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3661 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3721 gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3781 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3841 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca tcgcggcgtt
     3901 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     3961 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4021 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4081 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4141 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4201 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4261 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4321 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4381 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4441 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4501 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4561 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4621 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4681 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4741 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4801 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4861 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     4921 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     4981 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5041 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5101 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5161 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5221 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5281 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5341 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5401 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5461 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5521 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5581 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5641 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5701 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5761 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5821 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     5881 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     5941 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6001 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6061 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6121 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6181 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6241 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6301 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6361 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6421 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6481 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6541 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct ttctattttt
     6601 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6661 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6721 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6781 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6841 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     6901 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     6961 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7021 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7081 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7141 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7201 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7261 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7321 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7381 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7441 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7501 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7561 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7621 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7681 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7741 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7801 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7861 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     7921 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     7981 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8041 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8101 cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8161 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8221 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8281 attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8341 tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat atattactta
     8401 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8461 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8521 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8581 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8641 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8701 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8761 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8821 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     8881 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     8941 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9001 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9061 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9121 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9181 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9241 gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca acaccgctca
     9301 ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9361 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9421 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9481 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9541 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9601 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9661 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9721 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9781 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9841 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
     9901 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
     9961 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10021 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10081 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10141 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10201 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10261 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10321 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10381 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10441 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10501 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10561 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10621 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10681 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10741 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10801 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10861 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    10921 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    10981 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11041 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11101 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11161 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11221 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11281 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11341 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11401 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11461 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11521 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11581 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11641 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11701 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11761 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11821 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    11881 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc ccggttatcg
    11941 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12001 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12061 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12121 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12181 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12241 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12301 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12361 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgag
    12421 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12481 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12541 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12601 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12661 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12721 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12781 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12841 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    12901 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    12961 attatggata tagacttgcc cggaacagac ggttt
//
LOCUS       DAJTNW010000102        12905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNW010000102 DAJTNW010000000
VERSION     DAJTNW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO179_003330"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO179_003330"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5533280.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3223)
                     /locus_tag="OO179_003331"
                     /pseudo
     CDS             complement(1806..3223)
                     /locus_tag="OO179_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3377..3721)
                     /locus_tag="OO179_003332"
     CDS             complement(3377..3721)
                     /locus_tag="OO179_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5533281.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OO179_003333"
     CDS             complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OO179_003333"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5533282.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4711..5646)
                     /locus_tag="OO179_003334"
     CDS             complement(4711..5646)
                     /locus_tag="OO179_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5533283.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5726..6841
                     /gene="yneK"
                     /locus_tag="OO179_003335"
     CDS             5726..6841
                     /gene="yneK"
                     /locus_tag="OO179_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5533284.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6991..8181
                     /gene="ydeA"
                     /locus_tag="OO179_003336"
     CDS             6991..8181
                     /gene="ydeA"
                     /locus_tag="OO179_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5533285.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OO179_003337"
     CDS             complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OO179_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5533286.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9083..9517
                     /gene="marR"
                     /locus_tag="OO179_003338"
     CDS             9083..9517
                     /gene="marR"
                     /locus_tag="OO179_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5533287.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9537..9920
                     /gene="marA"
                     /locus_tag="OO179_003339"
     CDS             9537..9920
                     /gene="marA"
                     /locus_tag="OO179_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5533288.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9952..10170
                     /gene="marB"
                     /locus_tag="OO179_003340"
     CDS             9952..10170
                     /gene="marB"
                     /locus_tag="OO179_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5533289.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OO179_003341"
     CDS             complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OO179_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5533290.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11364..12482
                     /gene="ydeE"
                     /locus_tag="OO179_003342"
     CDS             11364..12482
                     /gene="ydeE"
                     /locus_tag="OO179_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5533291.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12609..12704
                     /gene="mgtS"
                     /locus_tag="OO179_003343"
     CDS             12609..12704
                     /gene="mgtS"
                     /locus_tag="OO179_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5533292.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3296 c         2950 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaatca tcggtcagcg
     1921 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
     1981 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
     2041 catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
     2101 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
     2161 cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca
     2221 tgaccgtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
     2281 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
     2341 gcaaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
     2401 taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa
     2461 tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg
     2521 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
     2581 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
     2641 tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag
     2701 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     2761 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     2821 caatcagaca gtaattaaat agccgtaaag cacttactgg ctcatactta tttttcccca
     2881 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     2941 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     3001 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
     3061 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     3121 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     3181 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     3241 aattgcgggt ggcaattatc tcaacgtgat aagtaaattc tcataaagga tagcatgttc
     3301 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     3361 ctcatggctg attcatctat acaaccagcc agtgatagat taccgccacg atataacgtt
     3421 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     3481 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     3541 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     3601 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     3661 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agatactgca
     3721 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     3781 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     3841 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     3901 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     3961 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     4021 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     4081 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     4141 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     4201 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg
     4261 cccttgcaac atatcgcaga ccaccagcgc accggtatta atgaacggat tacgcggaat
     4321 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     4381 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     4441 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     4501 gtcaacggta cagatagcaa tccccaatcg ggaaccgtct actgtagcca gcgccggaat
     4561 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     4621 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     4681 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ctgtctggat
     4741 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     4801 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     4861 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     4921 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt
     4981 gattgcagca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     5041 cgccagaacc gttggcggat agtagttacc tgctccagcc atcttttccc cgcccagtaa
     5101 caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
     5161 aaaacgagcc attggtccga gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc
     5221 tgccgcagct gacacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa
     5281 gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
     5341 aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc
     5401 cagtacgcat tttttcagtg ccgctccagc acgaacactt ccggtcaccg tgacagcagc
     5461 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
     5521 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
     5581 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
     5641 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
     5701 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
     5761 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
     5821 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
     5881 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
     5941 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
     6001 tgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
     6061 tatttattta gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc
     6121 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
     6181 tatgaatgat tttattgaac aatttccaat gatcaacctt ggcgccccca tcaaaagagc
     6241 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
     6301 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
     6361 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
     6421 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
     6481 ttcattaggt tctaacaaac atgtacaacc cgaagtctgc atctgcttct tatccctttt
     6541 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt
     6601 gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt tgatgccagc
     6661 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca aactgtttgt
     6721 aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc
     6781 ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg aacgtcatta
     6841 aaccgtgatg ttaccgactc tctgactcgg aacagaatca gcgtcagaga aacggaaaac
     6901 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
     6961 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
     7021 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
     7081 gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
     7141 ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
     7201 atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     7261 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
     7321 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
     7381 ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg
     7441 gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg aatgaccttc
     7501 ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta
     7561 ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     7621 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     7681 tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     7741 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
     7801 caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg tctggcactg
     7861 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
     7921 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct
     7981 accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg
     8041 ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg
     8101 gtgcctgctt ttgctgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
     8161 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
     8221 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc
     8281 catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga
     8341 gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
     8401 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
     8461 catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt
     8521 ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
     8581 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
     8641 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag cgtaatacgc
     8701 caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc
     8761 actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa
     8821 cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
     8881 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
     8941 actctttagc taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
     9001 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattaccc cctacaacta
     9061 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
     9121 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct
     9181 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
     9241 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
     9301 tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa
     9361 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggaa atatgcgaac aatgccatca
     9421 attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc
     9481 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt
     9541 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
     9601 tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc
     9661 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta
     9721 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
     9781 aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg
     9841 ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat
     9901 taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca
     9961 ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa
    10021 accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa
    10081 caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc
    10141 gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta
    10201 ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc
    10261 ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat ccaacaatag
    10321 tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaagt
    10381 ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc
    10441 cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt gaatcatgga
    10501 tgcggaacca tcgaaaatca gcgagacaac aaagaaggga atgattggga ttaaagcgct
    10561 ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa
    10621 gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc cgagcatcgc
    10681 cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc
    10741 gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat
    10801 agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt
    10861 aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat accccaagag
    10921 caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat
    10981 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    11041 aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat
    11101 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    11161 taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata
    11221 cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag aagggcgctg
    11281 gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta
    11341 ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt tacttgagtc
    11401 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    11461 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    11521 tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg
    11581 tgacgctggt tgtgctcttt tttgccctca ttaactgcgc ctattctgtt tttgctactg
    11641 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa atcttctcaa
    11701 tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc acgctgttgg
    11761 tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg tttcccatgc
    11821 ttttcattca aatatgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    11881 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggtt
    11941 ttctggcttc ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    12001 ttgctgatgg ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    12061 tggtggttac gttgcaatat tccgtgggcc gccgacttaa cccagctaac atccgcgcgc
    12121 tgatgacagc aggcaccctc tgtttcgtca tcggactggt cggttttatt ttttccggca
    12181 acaacctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    12241 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    12301 tttccgccca gtctttaggc tggcttggtg ccgcgattaa cccattagtg agtggcgtag
    12361 tgctaaccag cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg atcattgctg
    12421 cgtgggtgct gatgttaaaa gggattcgtg caagaccgtg ggggcagccc gcgctttgtt
    12481 gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttat ctctgctaat
    12541 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    12601 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    12661 ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    12721 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    12781 aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt
    12841 acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc
    12901 tcaat
//
LOCUS       DAJTNW010000103        12695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNW010000103 DAJTNW010000000
VERSION     DAJTNW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO179_003344"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO179_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5533293.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO179_003345"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO179_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5533294.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO179_003346"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO179_003346"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5533295.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO179_003347"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO179_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5533296.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO179_003348"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO179_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5533297.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO179_003349"
     CDS             4025..4615
                     /locus_tag="OO179_003349"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5533298.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO179_003350"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO179_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5533299.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO179_003351"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO179_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5533300.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO179_003352"
     CDS             complement(6551..6658)
                     /locus_tag="OO179_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533301.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO179_003353"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO179_003353"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5533302.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO179_003354"
     CDS             7648..7875
                     /locus_tag="OO179_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5533303.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO179_003355"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO179_003355"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5533304.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO179_003356"
     CDS             complement(9447..10703)
                     /locus_tag="OO179_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5533305.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO179_003357"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO179_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5533306.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO179_003358"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO179_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5533307.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO179_003359"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO179_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5533308.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3244 c         3198 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAJTNW010000104        12404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNW010000104 DAJTNW010000000
VERSION     DAJTNW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="OO179_003360"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="OO179_003360"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5533309.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="OO179_003361"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="OO179_003361"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5533310.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO179_003362"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO179_003362"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5533311.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="OO179_003363"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="OO179_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="OO179_003364"
     CDS             3602..3958
                     /locus_tag="OO179_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5533312.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="OO179_003365"
     CDS             4285..4944
                     /locus_tag="OO179_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5533313.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="OO179_003366"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="OO179_003366"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5533314.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO179_003367"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO179_003367"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5533315.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="OO179_003368"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="OO179_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO179_003369"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO179_003369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5533316.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="OO179_003370"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="OO179_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5533317.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="OO179_003371"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="OO179_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="OO179_003372"
     CDS             10287..10628
                     /locus_tag="OO179_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5533318.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="OO179_003373"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="OO179_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="OO179_003374"
     CDS             complement(12063..12344)
                     /locus_tag="OO179_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5533319.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3075 a         3032 c         3137 g         3160 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttg
//
LOCUS       DAJTNW010000105        12163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNW010000105 DAJTNW010000000
VERSION     DAJTNW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..433)
                     /locus_tag="OO179_003375"
     CDS             complement(<1..433)
                     /locus_tag="OO179_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5533320.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINF"
     gene            complement(464..1156)
                     /gene="fimC"
                     /locus_tag="OO179_003376"
     CDS             complement(464..1156)
                     /gene="fimC"
                     /locus_tag="OO179_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5533321.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1376..1918)
                     /gene="fimA"
                     /locus_tag="OO179_003377"
     CDS             complement(1376..1918)
                     /gene="fimA"
                     /locus_tag="OO179_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5533322.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2399..3265
                     /gene="folD"
                     /locus_tag="OO179_003378"
     CDS             2399..3265
                     /gene="folD"
                     /locus_tag="OO179_003378"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5533323.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3267..3479
                     /gene="ybcJ"
                     /locus_tag="OO179_003379"
     CDS             3267..3479
                     /gene="ybcJ"
                     /locus_tag="OO179_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5533324.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3587..4096
                     /locus_tag="OO179_003380"
     CDS             3587..4096
                     /locus_tag="OO179_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5533325.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4132..5517)
                     /gene="cysS"
                     /locus_tag="OO179_003381"
     CDS             complement(4132..5517)
                     /gene="cysS"
                     /locus_tag="OO179_003381"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5533326.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5691..6185
                     /gene="ppiB"
                     /locus_tag="OO179_003382"
     CDS             5691..6185
                     /gene="ppiB"
                     /locus_tag="OO179_003382"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5533327.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6188..6910
                     /gene="lpxH"
                     /locus_tag="OO179_003383"
     CDS             6188..6910
                     /gene="lpxH"
                     /locus_tag="OO179_003383"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5533328.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7028..7537
                     /gene="purE"
                     /locus_tag="OO179_003384"
     CDS             7028..7537
                     /gene="purE"
                     /locus_tag="OO179_003384"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5533329.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7534..8601
                     /gene="purK"
                     /locus_tag="OO179_003385"
     CDS             7534..8601
                     /gene="purK"
                     /locus_tag="OO179_003385"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5533330.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8738..9631)
                     /locus_tag="OO179_003386"
     CDS             complement(8738..9631)
                     /locus_tag="OO179_003386"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5533331.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9628..10275)
                     /locus_tag="OO179_003387"
                     /pseudo
     CDS             complement(9628..10275)
                     /locus_tag="OO179_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10278..11510
                     /gene="allC"
                     /locus_tag="OO179_003388"
     CDS             10278..11510
                     /gene="allC"
                     /locus_tag="OO179_003388"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5533332.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11521..>12163
                     /gene="allE"
                     /locus_tag="OO179_003389"
     CDS             11521..>12163
                     /gene="allE"
                     /locus_tag="OO179_003389"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5533333.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2952 a         3144 c         3102 g         2965 t
ORIGIN      
        1 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
       61 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      121 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      181 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      241 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      301 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      361 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      421 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      481 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      541 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      601 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      661 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      721 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      781 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      841 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      901 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
      961 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1021 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1081 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1141 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1201 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1261 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1321 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1381 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1441 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1501 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1561 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1621 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1681 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1741 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1801 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1861 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1921 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     1981 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2041 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2101 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2161 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2221 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2281 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2341 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2401 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2461 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2521 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2581 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2641 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2701 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2761 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2821 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2881 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     2941 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3001 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3061 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3121 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3181 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3241 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3301 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3361 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3421 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3481 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3541 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3601 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3661 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3721 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3781 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3841 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3901 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     3961 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4021 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4081 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4141 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4201 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4261 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4321 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4381 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4441 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4501 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4561 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4621 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4681 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4741 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4801 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4861 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4921 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     4981 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5041 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5101 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5161 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5221 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5281 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5341 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5401 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5461 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5521 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5581 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5641 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5701 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5761 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5821 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5881 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     5941 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6001 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6061 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6121 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6181 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6241 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6301 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6361 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6421 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6481 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6541 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6601 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6661 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6721 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6781 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6841 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6901 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     6961 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7021 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7081 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7141 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7201 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7261 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7321 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7381 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7441 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7501 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7561 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7621 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7681 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7741 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7801 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7861 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7921 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     7981 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8041 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8101 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8161 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8221 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8281 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8341 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8401 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8461 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8521 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8581 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8641 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8701 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8761 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8821 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8881 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     8941 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9001 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9061 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9121 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9181 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9241 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9301 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9361 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9421 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9481 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9541 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9601 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9661 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9721 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9781 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9841 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9901 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
     9961 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10021 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10081 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10141 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10201 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10261 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10321 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10381 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10441 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10501 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10561 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10621 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10681 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10741 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10801 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10861 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10921 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    10981 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11041 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11101 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11161 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11221 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11281 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11341 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11401 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11461 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11521 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11581 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11641 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11701 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11761 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11821 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11881 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    11941 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12001 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12061 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12121 gaacatggtg cctatattct ttccggtcag ggggtttata acc
//
LOCUS       DAJTNW010000106        11921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNW010000106 DAJTNW010000000
VERSION     DAJTNW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..2338)
                     /locus_tag="OO179_003390"
     CDS             complement(<1..2338)
                     /locus_tag="OO179_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5533334.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="OO179_003391"
     CDS             complement(2272..2895)
                     /locus_tag="OO179_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5533335.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="OO179_003392"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="OO179_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OO179_003393"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OO179_003393"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5533336.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="OO179_003394"
     CDS             7503..8225
                     /locus_tag="OO179_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5533337.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OO179_003395"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OO179_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5533338.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="OO179_003396"
     CDS             complement(10297..11892)
                     /locus_tag="OO179_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5533339.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2946 g         2911 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccggg tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAJTNW010000107        11715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNW010000107 DAJTNW010000000
VERSION     DAJTNW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            127..996
                     /locus_tag="OO179_003397"
     CDS             127..996
                     /locus_tag="OO179_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5533340.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="OO179_003398"
     CDS             complement(1199..1552)
                     /locus_tag="OO179_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5533341.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OO179_003399"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OO179_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5533342.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="OO179_003400"
     CDS             complement(3380..3673)
                     /locus_tag="OO179_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5533343.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="OO179_003401"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="OO179_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5533344.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OO179_003402"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OO179_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5533345.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="OO179_003403"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="OO179_003403"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5533346.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /gene="dcuA"
                     /locus_tag="OO179_003404"
     CDS             7588..8889
                     /gene="dcuA"
                     /locus_tag="OO179_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5533347.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="OO179_003405"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="OO179_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5533348.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /gene="dsbD"
                     /locus_tag="OO179_003406"
     CDS             9319..11016
                     /gene="dsbD"
                     /locus_tag="OO179_003406"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5533349.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="OO179_003407"
     CDS             11053..11628
                     /locus_tag="OO179_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5533350.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2813 a         3024 c         2882 g         2996 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcatccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaagcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga cgctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgccttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagcc atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag agctcatcat agttttgctg
     7621 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgagc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact caaacgcatc tcaactttac acaaatcaaa
    10681 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgctgcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gaaatgtgca gatta
//
LOCUS       DAJTNW010000108        11666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNW010000108 DAJTNW010000000
VERSION     DAJTNW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            597..794
                     /locus_tag="OO179_003408"
     CDS             597..794
                     /locus_tag="OO179_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533351.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO179_003409"
     CDS             complement(744..1937)
                     /locus_tag="OO179_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5533352.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO179_003410"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO179_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5533353.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO179_003411"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO179_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5533354.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO179_003412"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO179_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5533355.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO179_003413"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO179_003413"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5533356.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO179_003414"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO179_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5533357.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO179_003415"
     CDS             complement(10573..10716)
                     /locus_tag="OO179_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533358.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11666
                     /locus_tag="OO179_003416"
     CDS             10966..>11666
                     /locus_tag="OO179_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5533359.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2961 a         2898 c         2993 g         2814 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atggga
//
LOCUS       DAJTNW010000109        11635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNW010000109 DAJTNW010000000
VERSION     DAJTNW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(282..357)
                     /locus_tag="OO179_003417"
     tRNA            complement(282..357)
                     /locus_tag="OO179_003417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO179_003418"
     CDS             916..3168
                     /locus_tag="OO179_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533360.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="OO179_003419"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="OO179_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5533361.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="OO179_003420"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="OO179_003420"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5533362.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="OO179_003421"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="OO179_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="OO179_003422"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="OO179_003422"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR5533363.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="OO179_003423"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="OO179_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5533364.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="OO179_003424"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="OO179_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5533365.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO179_003425"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO179_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5533366.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="OO179_003426"
     CDS             complement(11389..11547)
                     /locus_tag="OO179_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5533367.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2993 a         2856 c         2900 g         2886 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttca
//
LOCUS       DAJTNW010000110        10929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNW010000110 DAJTNW010000000
VERSION     DAJTNW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            156..506
                     /locus_tag="OO179_003427"
     CDS             156..506
                     /locus_tag="OO179_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5533368.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            559..954
                     /locus_tag="OO179_003428"
     CDS             559..954
                     /locus_tag="OO179_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5533369.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="OO179_003429"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="OO179_003429"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5533370.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="OO179_003430"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="OO179_003430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5533371.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="OO179_003431"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="OO179_003431"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5533372.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="OO179_003432"
     CDS             complement(5325..6425)
                     /locus_tag="OO179_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5533373.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="OO179_003433"
     CDS             complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="OO179_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5533374.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7573..8139)
                     /locus_tag="OO179_003434"
     CDS             complement(7573..8139)
                     /locus_tag="OO179_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5533375.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8355..10088
                     /gene="argS"
                     /locus_tag="OO179_003435"
     CDS             8355..10088
                     /gene="argS"
                     /locus_tag="OO179_003435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5533376.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10265..10753
                     /locus_tag="OO179_003436"
     CDS             10265..10753
                     /locus_tag="OO179_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5533377.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2671 a         2790 c         2605 g         2863 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt
      721 cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgtatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat
     6541 gatttattac aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatata
     6601 tatttatcag ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta
     6661 gcacaacgct atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat
     6721 actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca
     6781 tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt
     6841 cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt
     6901 tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct
     6961 cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca
     7021 cggactcctg ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt
     7081 tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc
     7141 aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga
     7201 aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca
     7261 ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg
     7321 cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt
     7381 gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt cagtaccccc
     7441 aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg
     7501 ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt
     7561 tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg
     7621 aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg
     7681 ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga
     7741 aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat
     7801 atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct ccacattgga
     7861 aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc
     7921 attgatcatt ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc
     7981 agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc
     8041 caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc
     8101 aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt
     8161 aaaagcgtta atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct
     8221 gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg
     8281 cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa
     8341 tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga
     8401 ttgcggcagg cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt
     8461 tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc
     8521 gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg
     8581 agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg
     8641 ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg
     8701 tggttgacta ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta
     8761 ccattattgg tgacacagca gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg
     8821 caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc
     8881 agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg
     8941 cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa
     9001 aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca
     9061 tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga
     9121 tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag
     9181 gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg
     9241 aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca
     9301 ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt
     9361 acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag
     9421 gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag
     9481 acggcaaacc gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac ctgctggatg
     9541 aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg
     9601 agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca
     9661 aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata
     9721 ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa
     9781 ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac
     9841 tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc
     9901 cgcatgtaat gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc
     9961 actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac
    10021 aactgacagc gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc
    10081 gtatgtaatc gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt
    10141 tattgccatc taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa
    10201 gaggccagaa gcgtaatatc caggcccagg aaaacgataa cggttcaatt taaggaatac
    10261 cgaagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga
    10321 agatacggtt atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa
    10381 ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatct
    10441 cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag aacaagtgcc
    10501 acgcacaggt gcaaaaaaac ttgatactga acaacttaac ttgcttaaaa aatcacagcg
    10561 agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg aggatgttca
    10621 ggatttaagt gacccctatt ctgaatcaga atggctatca tgtatgatca tacagaccaa
    10681 tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc caagcccttt
    10741 gacaagagga taattcacat ctttttggca tgttttgttg caagctattc ctgataaatg
    10801 attgcaacaa aacatccagc cttttgactg agttattaaa catactcgcg agcgcgtaat
    10861 tttttgtctt tcagcgataa ttcacaatca cttcattccg ttgtaccttt agcggtggaa
    10921 ttaaccgcc
//
LOCUS       DAJTNW010000111        10916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNW010000111 DAJTNW010000000
VERSION     DAJTNW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="OO179_003437"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="OO179_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5533378.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OO179_003438"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OO179_003438"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5533379.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="OO179_003439"
     CDS             complement(2271..3686)
                     /locus_tag="OO179_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5533380.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OO179_003440"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OO179_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5533381.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OO179_003441"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OO179_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10916)
                     /gene="mdtA"
                     /locus_tag="OO179_003442"
     CDS             complement(9875..>10916)
                     /gene="mdtA"
                     /locus_tag="OO179_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5533382.1"
                     /translation="LIGARYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGD
                     LLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQAL
                     VSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVIT
                     QTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATT
                     GTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSEN
                     KVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKA
                     TSREYAKKGARS"
BASE COUNT         2513 a         3074 c         3006 g         2323 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttactg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta acgtgcccct attaag
//
LOCUS       DAJTNW010000112        10915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNW010000112 DAJTNW010000000
VERSION     DAJTNW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..203
                     /locus_tag="OO179_003443"
     CDS             <1..203
                     /locus_tag="OO179_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533383.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OO179_003444"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OO179_003444"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5533384.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OO179_003445"
     CDS             2102..2818
                     /locus_tag="OO179_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5533385.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="OO179_003446"
     tRNA            complement(3274..3349)
                     /locus_tag="OO179_003446"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO179_003447"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO179_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="OO179_003448"
     tRNA            5082..5157
                     /locus_tag="OO179_003448"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO179_003449"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO179_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5533386.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO179_003450"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO179_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5533387.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="OO179_003451"
     tRNA            7492..7567
                     /locus_tag="OO179_003451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO179_003452"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO179_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5533388.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO179_003453"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO179_003453"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5533389.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO179_003454"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO179_003454"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5533390.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10915)
                     /gene="cobU"
                     /locus_tag="OO179_003455"
     CDS             complement(10451..>10915)
                     /gene="cobU"
                     /locus_tag="OO179_003455"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5533391.1"
                     /translation="LYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHAD
                     INPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAK
                     VVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3031 a         2780 c         2362 g         2742 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacag
//
LOCUS       DAJTNW010000113        10706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNW010000113 DAJTNW010000000
VERSION     DAJTNW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO179_003456"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO179_003456"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5533392.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO179_003457"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO179_003457"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5533393.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO179_003458"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO179_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5533394.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO179_003459"
     CDS             6038..6658
                     /locus_tag="OO179_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5533395.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO179_003460"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO179_003460"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5533396.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO179_003461"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO179_003461"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5533397.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO179_003462"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO179_003462"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5533398.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO179_003463"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO179_003463"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5533399.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..10706)
                     /gene="ttdR"
                     /locus_tag="OO179_003464"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..10706)
                     /gene="ttdR"
                     /locus_tag="OO179_003464"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2501 a         3108 c         2640 g         2457 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggata
//
LOCUS       DAJTNW010000114        10665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNW010000114 DAJTNW010000000
VERSION     DAJTNW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OO179_003465"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OO179_003465"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5533400.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="OO179_003466"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="OO179_003466"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5533401.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4036..4278
                     /locus_tag="OO179_003467"
     CDS             4036..4278
                     /locus_tag="OO179_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533402.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4392..5030
                     /locus_tag="OO179_003468"
     CDS             4392..5030
                     /locus_tag="OO179_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5533403.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5027..5764
                     /locus_tag="OO179_003469"
     CDS             5027..5764
                     /locus_tag="OO179_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5533404.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5764..7860
                     /locus_tag="OO179_003470"
     CDS             5764..7860
                     /locus_tag="OO179_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5533405.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7907..8185)
                     /locus_tag="OO179_003471"
     CDS             complement(7907..8185)
                     /locus_tag="OO179_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533406.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8186..8329
                     /locus_tag="OO179_003472"
     CDS             8186..8329
                     /locus_tag="OO179_003472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533407.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8455..8865
                     /gene="psiE"
                     /locus_tag="OO179_003473"
     CDS             8455..8865
                     /gene="psiE"
                     /locus_tag="OO179_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5533408.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OO179_003474"
     CDS             complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OO179_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5533409.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2710 a         2722 c         2763 g         2470 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca agttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 tttttatttt aagcttatga tttttattgt tatttaaata taagttgaaa cttatatttg
     3901 atattcattc caattatcct aaaactccat cgctaattcc ccgcgccgta attcgcatgc
     3961 tttagttgtg tatactcgat cccgcccgaa atgtttttgg gtaaatctcc attcattcaa
     4021 tgaagggaaa ttgttatgaa aaaagttctg tatggcattt ttgccatatc tgcgcttgcg
     4081 gcgacttctg cgtgggctgc acctgtacag gtgggcgaag cggcagagtc ggcagcaacg
     4141 tcggtttcgg cggggagttc ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt
     4201 gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata ccgggaccat cacaaccacg
     4261 accaccagta cccagtaata aagtatgtat ccccaaaata atttgagtca ttgcatctgt
     4321 ggcttgaagt atgaagggat ttaccataac cacactccgg tgtggttatt ctgcccctct
     4381 ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta caggcctgtt
     4441 cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggc acgccaggcg
     4501 tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac atgcagctta
     4561 atggcgggcc gcaattgttt gtggtgctgg ccttcgctga agacggacaa caaaaatggg
     4621 tcactcagga tcaagccact cttgtcacac aacatggtcg tctggtgaag actttgcttg
     4681 gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt aaacccgcac
     4741 aaattgttga tggtgcaaca tggacgcgca cgatgggctg gaccgagtac cagcaggtac
     4801 gctacgccac cgcacgctca gtcttcaaat gggatggcac cgataccgtc aaggtcggca
     4861 gcgatgaaac cctggttcgc gtgctggacg aagaagtctc caccgaccag gcgcgctggc
     4921 ataaccgcta ctggatcgac agcgaagggc aaattcgcca gtcggaacag tatctcggcg
     4981 cggattattt cccggtgaaa accactctca tcaaggcggc aaaacaatga ttaaacaaac
     5041 tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa
     5101 ggtgttcagc aatggcagca gtgaggccaa aacgctaacg ggcgcagagc atttaatcga
     5161 tctggtaggg caacctcgac tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga
     5221 gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca
     5281 gggcgcagat tccagcaccg acgatgccgc cgccattaac gccttacgcc agcaaatcca
     5341 ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc
     5401 cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggac caccgccgtc
     5461 cacggtcacg ttgttcgggc ttatcagcca tcctggcaat cagccattca ctccaggtcg
     5521 cgacgtggcg agctatctct ctgaccagag cctgctcagc ggtgcggatc gcagctacgc
     5581 gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg gtggcttact ggaacaagcg
     5641 tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcagatt ccgtctggag
     5701 tgagacgcct gatgccctta acgccgacat tcttcagact ctgacgcagc ggatacccca
     5761 ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac agcttgctac
     5821 ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg atttcggtgg cgtaggatta
     5881 ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat
     5941 aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct ggaaacaacg
     6001 ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt ctctggcgat
     6061 caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga gagttactgg
     6121 ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt tgatgcggaa
     6181 tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct gggctgggga
     6241 tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt cagccagtga taaatattgc
     6301 tatcgcgata acagctacaa acaggcggga tctatcgacg gtagccagat gttccacggt
     6361 cctgcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct gcgtctgaaa
     6421 ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga gcaaaaaagt
     6481 aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa ccttagctat
     6541 gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc
     6601 ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc agccgcagga tgccattttg
     6661 cagcattcgg tagtggcgaa tcagttaacg ttgctgaaat acaacgccgg acttgccgat
     6721 ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt
     6781 gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct gccggatggg
     6841 atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaagtgac gacggaaacc
     6901 gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt tgggccacga aacgacgctg
     6961 gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg gtatatcgac
     7021 aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa
     7081 aacttttaca tgtatcagct gggcgtgatg ggaacggcag atttgtggct gacggaccat
     7141 ctactgacca ccggcagcct gtttgcaaat cttgccaaca actacgacaa gtttaactac
     7201 accaatccac cgcaggactc gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg
     7261 cagaacgatg tctatgtgaa taacctgcaa gccaactact tccagcatct gggcaacggc
     7321 ttctacggtc aggtctatgg aggttatctc gaaaccatgt ttggcggtgc gggggccgaa
     7381 gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa
     7441 cagcgcgact ggcgtagtgc aaaagatatg atgaaattca ccgactacag cgtgaaaacc
     7501 ggacatctga ccgcctactg gacgccatct ttcgcccagg acgtgttggt gaaagccagc
     7561 gtcgggcagt atctggcagg ggataaaggc ggcacgctgg agatcgctaa acgctttgac
     7621 agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg tttcgaaaga ggagtacggc
     7681 gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc
     7741 ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg tggtcagcaa
     7801 cttgggcgta agttccagtt gtatgacatg accagcgacc gtagcgtaaa tttccgctaa
     7861 gtcatgggaa aggtgccagt tttcgcactc actggcaccc tcattcttaa tgacctgttg
     7921 agccatcagg aacgcagttc acccccggtt tacgatctgg tgtgtaaagg cgaaaacatt
     7981 ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac tgggccatca
     8041 ttgggctgga gacattgctt tgcggtaggt cggctttatc agcagtaccg agcggaccag
     8101 cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc ttcattttta
     8161 ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat
     8221 tgctggtggc tgggaatcac ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt
     8281 tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc tacgggtagg tatatccggc
     8341 tttggtggag gcgcgctcca aatccaggtt gaacaaaaca tacacaaaaa atatagatct
     8401 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     8461 tcactctctc gtccgcgcgt ggagtttatc tccaccattt tgcagaccgt actcaatctt
     8521 ggcctgctgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     8581 gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg agctggtaga aggactggtg
     8641 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     8701 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     8761 gtcgatcata aatcgccact ggatgtgttg atctactcgg ctgcgatcct gttactggtg
     8821 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg
     8881 aaatggcgcg gcgtgctgga caggaagatt acagcgtagc agtttgttgt gttttcttcg
     8941 tttccggttc ccagagcgct tccagctcct caagggtttt acctttggtt tccgggacaa
     9001 atttccacat aaacagtgct gccagaacgc ccatacaacc gtaaatccag taggagaaac
     9061 cgttgtggaa atgggccacc agccaggagt ttttgtccat catcgggaag gtccaggaga
     9121 cgaagtagtt cgccagccac tgggccgcca ccgcgattgc cagcgcttta ccacgaatag
     9181 cattcgggaa gatttccgac agcagtaccc agcataccgg accccaggac atggcaaagg
     9241 cggcaacata gaacagcatc gacagtagcg ccacaatacc cgatgcctga gtgtaaaacg
     9301 cggtaccgag gctaaacata ccgattgcca ttccgagtgc gccgataatt tgcagtggct
     9361 tacgaccaaa tttatccaca gtcataattg ccagtacggt aaaggtgagg ttgataactc
     9421 cgacaataat ggtctgcaac agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt
     9481 ccggcgcgta gtacagcacc acattgatgc cgacaaattg ctggaagatg gagagcataa
     9541 cgccgattac aatcacgccc acgccaaaca tcagcagacg accaccggtt ttgcggccat
     9601 gatccaggga gtgtttaatt tcctgtactg cctgagttgc aagcgtgttg cccataattt
     9661 tgcgcaggat actttccgtc tgttcttgct tgccgcgtga catcagccag cgaggacttt
     9721 ctggcacggt atacagcagc attaagaaca gcagtgcagg gatacattcc gaggcaaaca
     9781 tataacgcct gccgtcagta ttcagccagc tggcatcacc ggaacgggca ataaaatagt
     9841 ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt
     9901 ttccgcgaat atgagctgga gccagttccg caatatacat tggtgaaagc attgaggcta
     9961 aaccaacgcc aataccgcca ataatacgat aaataacaaa ttccgggaca taacctgcca
    10021 gataaatagg tactgtgttg tccgggttta tagaggtaaa accaagttct ggccaggcag
    10081 aacctacacc agaaataaaa aacaggacag cagaaatctt aagtgaatca cgacggccga
    10141 agcggttact gcaataacca ccgagggcac cgccgatgat gcaaccaatc agagcgctgg
    10201 ctacgcaaaa tcctaacagg gagttggcag cggattcact taagttttgt ggagcaacaa
    10261 agacggtatt gagtgactca acagtaccgg aaataacggc ggtgtcgtag ccaaataata
    10321 aaccacctaa tgtagcgact aaggtaatcg aaaatatata actggaatta tactgggtat
    10381 tcattcagac ctgccttaga ccattctgat cttttatcag tgcattgatg ttggaattgt
    10441 caggcagtta aggcaattat cattttttac aatgcgttta cgtaatgtct tttctgtgat
    10501 cttaattgtg ataattatca aaaattgatg aaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aaaaa
//
LOCUS       DAJTNW010000115        10629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNW010000115 DAJTNW010000000
VERSION     DAJTNW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            51..1541
                     /locus_tag="OO179_003475"
     CDS             51..1541
                     /locus_tag="OO179_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5533410.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO179_003476"
     CDS             1593..2750
                     /locus_tag="OO179_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5533411.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO179_003477"
     CDS             complement(2716..2838)
                     /locus_tag="OO179_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533412.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO179_003478"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO179_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO179_003479"
     CDS             4764..7124
                     /locus_tag="OO179_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5533413.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQETRKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..9964
                     /locus_tag="OO179_003480"
     CDS             7181..9964
                     /locus_tag="OO179_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5533414.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10325..>10629
                     /locus_tag="OO179_003481"
     CDS             10325..>10629
                     /locus_tag="OO179_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5533415.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3152 a         2343 c         2295 g         2839 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaaacgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7381 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7441 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7501 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7561 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7621 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7681 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7741 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7801 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7861 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     7921 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     7981 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8041 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8101 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8161 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8221 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8281 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8341 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8401 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8461 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8521 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8581 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8641 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8701 tagcagcaaa gaaacactgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8761 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8821 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     8881 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     8941 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9001 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9061 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9121 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9181 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9241 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9301 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9361 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9421 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9481 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9541 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9601 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9661 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9721 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9781 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9841 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
     9901 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
     9961 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10021 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10081 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10141 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10201 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10261 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10321 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10381 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10441 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10501 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10561 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10621 ttgcgtaaa
//
LOCUS       DAJTNW010000116        10544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNW010000116 DAJTNW010000000
VERSION     DAJTNW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(109..381)
                     /locus_tag="OO179_003482"
                     /pseudo
     CDS             complement(109..381)
                     /locus_tag="OO179_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(475..1037)
                     /locus_tag="OO179_003483"
                     /pseudo
     CDS             complement(475..1037)
                     /locus_tag="OO179_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1241..1447)
                     /locus_tag="OO179_003484"
     CDS             complement(1241..1447)
                     /locus_tag="OO179_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5533416.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1554..2117)
                     /locus_tag="OO179_003485"
     CDS             complement(1554..2117)
                     /locus_tag="OO179_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5533417.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3492..5327)
                     /locus_tag="OO179_003486"
     CDS             complement(3492..5327)
                     /locus_tag="OO179_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533418.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5428..5715
                     /locus_tag="OO179_003487"
     CDS             5428..5715
                     /locus_tag="OO179_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5533419.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5687..7216
                     /locus_tag="OO179_003488"
     CDS             5687..7216
                     /locus_tag="OO179_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5533420.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7386..8603)
                     /locus_tag="OO179_003489"
     CDS             complement(7386..8603)
                     /locus_tag="OO179_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533421.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8970..9956)
                     /locus_tag="OO179_003490"
     CDS             complement(8970..9956)
                     /locus_tag="OO179_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533422.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9953..10481)
                     /locus_tag="OO179_003491"
                     /pseudo
     CDS             complement(9953..10481)
                     /locus_tag="OO179_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3283 a         2416 c         1989 g         2856 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcact tacaccagac
      121 atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca cagcttccct
      181 actccagagg taatataaag gggtgggctc gactgattta tctttacgtc gcttacactg
      241 caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca gagaaggaat
      301 tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa ttttaggatg
      361 tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg cgttacagga
      421 aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa taggtcaaaa
      481 accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg tcaaataatc
      541 cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa tatccaggat
      601 gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt ccaaccctaa
      661 ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct catagtttcc
      721 cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca tttaaaggac
      781 tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg tttgccttgt
      841 tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg gaaacgagat
      901 atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc aatcacagga
      961 gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat attcgtattg
     1021 atagttctca gagatatatc acactctaga tattttaatt aagtatcagt tcgatatcgg
     1081 catattgtac actacctcag gtagatgatg agatcagata cagggatcaa ctgacggtga
     1141 gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca ttcgttagcc
     1201 aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg gcccatacat
     1261 catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg gataatacga
     1321 aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga gcttcaagta
     1381 atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg tcagatttat
     1441 aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg aaattcgtgt
     1501 gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat aattcagtta
     1561 tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag aataataccg
     1621 tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc gtctaccgca
     1681 gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc tttagcccgg
     1741 cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc cagttgcctg
     1801 gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa atgaatatga
     1861 agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat cgtctgggtt
     1921 tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg cccgaaactg
     1981 ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga aaaacgttga
     2041 aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat tttgtaattg
     2101 ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa gagagaatta
     2161 cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa gtaaaccagc
     2221 agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg aggtaatact
     2281 tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat ggaaaaacac
     2341 acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag taatctgaca
     2401 ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt gtcgaaccat
     2461 taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa taatggtata
     2521 cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca tatttaccta
     2581 tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata cgtaaccagt
     2641 gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc ccccatatgc
     2701 ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact tagcataaag
     2761 aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa caatacggca
     2821 cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt atttatggta
     2881 tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat gatcactgta
     2941 taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga gtgaccagta
     3001 atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa gccactaaca
     3061 taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc atgatttggc
     3121 ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa gagcttacac
     3181 cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac aatgaacatg
     3241 caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga tggcgctaag
     3301 aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa aaacaaaaca
     3361 aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa tacccctgct
     3421 aattttgcta atggttagca aaattagcag ggtatataaa gattattgac ccaaaacaca
     3481 ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc acaaataatg
     3541 gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc gccatacaac
     3601 atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa tacaccaagc
     3661 ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc tatcatagtt
     3721 tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc cagcgtctta
     3781 ccatcataaa catactcttt atctatcaga atatttatct tatccagcac cttctctacg
     3841 tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact gatcttttca
     3901 agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc atcatactgt
     3961 atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa tgcatcctga
     4021 taggctttta catggtcaag ccaataaaac aacgagccat actttatatt ttttctattt
     4081 actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata tcctgaaggt
     4141 aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac atcattaatt
     4201 ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct ggtcacatga
     4261 acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata tgcgtcatca
     4321 caaccattct tatcattaat attaaataca agatctttca gacgggaaat atattcctga
     4381 gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact gttttgaaac
     4441 ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct ggtaaagtca
     4501 taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc tctcaccaac
     4561 actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat atcaagtaag
     4621 acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac tttctccagt
     4681 tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt gatagccatg
     4741 atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt tcctgtatat
     4801 ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa tcccagttca
     4861 gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt atcagcgtga
     4921 tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata aaaagcagag
     4981 tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac cccaaaagtg
     5041 cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg ataagaattc
     5101 atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc ctgactgaac
     5161 accccttcag catcaataat acgactgatc tcattataac gttgatccca gaatttttga
     5221 tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc cttaaacaac
     5281 cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg ctgtgctctc
     5341 cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa actgacacaa
     5401 cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct ttacacgcct
     5461 tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag atctactatt
     5521 tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa accaataagg
     5581 cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct gatgttacat
     5641 acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga tgacgacaaa
     5701 actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg cattacttga
     5761 tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta tgctgacggt
     5821 catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc aacgtaccag
     5881 ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc
     5941 aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat atatcgacga
     6001 taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa tcctggagcg
     6061 gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac gagcagcact
     6121 tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca tcgtgaatga
     6181 cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc tgttgctgtc
     6241 tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac tgtacaacac
     6301 attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt tccggctgaa
     6361 tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata agttttttac
     6421 tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat tttgcgaacc
     6481 cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg tgacgcaaca
     6541 atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact ctctgagagc
     6601 atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat caaatgcagc
     6661 atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac agaatcctca
     6721 tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca ggatagcagc
     6781 acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa gtactaatag
     6841 cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg aatcccttcg
     6901 ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta tcacaacagc
     6961 aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc tcaggaaaaa
     7021 atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa ggattgatga
     7081 taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa aaaaactaaa
     7141 tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac tgaagtatca
     7201 ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg tattattcgt
     7261 tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg aactggcatt
     7321 gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta tgccagtcca
     7381 tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata atccgcccat
     7441 gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc cgcctcaacc
     7501 ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata cccctgttca
     7561 ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg
     7621 tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc
     7681 tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt ttccagaatg
     7741 taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat tctttctgca
     7801 ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc cctggcttcg
     7861 cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac attataaggt
     7921 tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg cattgcgggt
     7981 tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac atcaacagga
     8041 tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat gcgctgttta
     8101 acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc tgccacgtcc
     8161 gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta attctcaagc
     8221 gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag ttctgcatgt
     8281 acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc agccttttta
     8341 atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc agtacgtgca
     8401 gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct tttttgtgtc
     8461 accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga aggatgaagg
     8521 gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat atgtcttgct
     8581 ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc catccagtat
     8641 gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac aaaaagtgta
     8701 atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt tatttcaggc
     8761 ggtccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg
     8821 tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga aattgtgcac
     8881 atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca gaacatatat
     8941 gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca acggaaatac
     9001 atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa aaaatagtaa
     9061 attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg ccattccaca
     9121 agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg caatatgaat
     9181 aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac
     9241 agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga
     9301 aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat tatcgattta
     9361 aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg gctgatagta
     9421 agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt cagactttta
     9481 tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt gcggatcatc
     9541 cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag cgcatataac
     9601 gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa cgacagaaat
     9661 cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt tttcttaaac
     9721 cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt gagatcaaaa
     9781 ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc ctctgatgga
     9841 aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat agcataaatg
     9901 tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg aatcatttga
     9961 atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga tccacgccag
    10021 caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg
    10081 gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt atgagttaag
    10141 tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc
    10201 atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc
    10261 tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt
    10321 tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg cgttccccca
    10381 ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta
    10441 atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta cccagcaata
    10501 gtggacacgc ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAJTNW010000117        10176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNW010000117 DAJTNW010000000
VERSION     DAJTNW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            491..622
                     /locus_tag="OO179_003492"
     CDS             491..622
                     /locus_tag="OO179_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5533423.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            882..1877
                     /gene="pgl"
                     /locus_tag="OO179_003493"
     CDS             882..1877
                     /gene="pgl"
                     /locus_tag="OO179_003493"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5533424.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1918..2871)
                     /locus_tag="OO179_003494"
                     /pseudo
     CDS             complement(1918..2871)
                     /locus_tag="OO179_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3055..4107
                     /locus_tag="OO179_003495"
     CDS             3055..4107
                     /locus_tag="OO179_003495"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5533425.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4183..5616
                     /locus_tag="OO179_003496"
     CDS             4183..5616
                     /locus_tag="OO179_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5533426.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5799..7979
                     /locus_tag="OO179_003497"
     CDS             5799..7979
                     /locus_tag="OO179_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5533427.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8120..9403)
                     /locus_tag="OO179_003498"
     CDS             complement(8120..9403)
                     /locus_tag="OO179_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5533428.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9538..>10176)
                     /locus_tag="OO179_003499"
     CDS             complement(9538..>10176)
                     /locus_tag="OO179_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533429.1"
                     /translation="STLSDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKI
                     YQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTL
                     HVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDP
                     PTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2509 a         2349 c         2548 g         2770 t
ORIGIN      
        1 atggatatgg aattagattg tgaataaatt gtatttaatg tatctctcct aatgaggagg
       61 gtgtttcaga atattcgcca taagggaatg agtgttatat catgctttct accctggtaa
      121 gtatatttgc ttttttcgta tatttggttc atcttttttt gatgttttac atattcacaa
      181 tgcagaggat ttataccaga ggacattagt cttgagtaag atactacaac atctccaact
      241 attttagcca gtctgactac ttgacttggt aatatttgtt gctaataatc atgtataaaa
      301 taagcttata tacatagtaa cttcagcaaa ggatttagta atgatcaaaa tcatatctca
      361 atgtactgtt tttttcttta aaaaaacagt acattctatt ttataatgac tgtgtattcc
      421 catggaagga gatcacccaa ttctcccccc cgacttttga cgcagtgtct tgttaacagg
      481 gggattgacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga ataccgatct
      541 acaacgtaat gcgtttattt gtgccggaag tgagttgatt gttgcgcgaa tgggaagttg
      601 gccagaagtt gttatggcat agtgtttacg gtcgttgata caggtaaaca aaagcgggga
      661 gagtcatact ccctgaatag tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg
      721 actaatattt tagtaatatg ccattcgaaa tggagtgcta acttattgat ttaattgatg
      781 tgcgatattg tattttatcg tgttttgttg tctgtttttt atccagtaag tgttgttata
      841 tgtatgaaag ataagggatt tatcattttt gaggtgtgtt catgaagcaa acagtttata
      901 tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga
      961 cgctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca
     1021 aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc
     1081 cggacgatgg cgcactgacc tttgccgcag agtctgtgct gccgggtagt ccgacgcata
     1141 tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga
     1201 gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtagtc gaggggctgg
     1261 acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa
     1321 agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctggtg gcgcaggacc
     1381 ctgcggaagt gactaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg
     1441 aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag
     1501 atccgcacgg caatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg
     1561 acacccgttg ggcggctgat attcatatca ccccggatgg tcgccattta tacgcctgcg
     1621 accgtaccgc cagtctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta
     1681 aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat tacagcggca
     1741 agtatctgat tgccgctggg caaaaatctc accacatctc ggtatacgaa attgttggcg
     1801 agcaggggct actgcatgaa aaaggccgct atgcggtcgg gcagggacca atgtgggtgg
     1861 tggttaacgc acactaaccg ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta
     1921 acttatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt
     1981 atcgtcagtc cacaaattaa tcagcaagga aaaaaagcgt tcagctgccg gagaaagcac
     2041 ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat
     2101 accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc
     2161 cagtcctgcc tccactagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc
     2221 cagcttccag ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cgctggagga
     2281 acgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct actttttggc
     2341 caatggatgg tcacgccgac aggccagcac gaatggctcg ttgaccagtg gggtaaaatc
     2401 aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac
     2461 cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag
     2521 ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc
     2581 taatgtcacc atatcacgat gataagcatt catatcacga atattaaaaa gcgtttcatc
     2641 gaatttcttt attaattccc gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt
     2701 aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat
     2761 tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaatgaact
     2821 ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc
     2881 ggaaaatgga agcgacgatt ttggggggct ggtcgttaaa aattttaact gcatttagcc
     2941 aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg
     3001 tccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
     3061 aaaataccct gcgtgatgat gcgaggtgga acctcgaggg ccgcgttcct gttagcggaa
     3121 catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt
     3181 aacgatctgg aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt
     3241 attagccgtt ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttatcgtc
     3301 catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg cgtaggggca
     3361 ttcgccattg aaaatggttt gattgcagcg acttcgccag ttacccgtgt acgtatccgc
     3421 aacgtcaata cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag
     3481 tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact
     3541 ttcctgaatg ccgctggaac gaaaaccgga aaagttttcc cgactgataa tcagattgat
     3601 tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg
     3661 gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca
     3721 ttattagccc gcattgaatc tatccggcta caagcgggta aagcaatggg cttaggtgat
     3781 gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
     3841 attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
     3901 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
     3961 gtaggatatg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
     4021 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacaac cagaaaaata
     4081 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata
     4141 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
     4201 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
     4261 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtgggtctg
     4321 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg
     4381 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
     4441 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
     4501 ggtttaggta aacttattgc ctatctgctg attggtaaaa tcggtaacac catgctgggt
     4561 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
     4621 gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcgatggc tttggggtcc
     4681 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
     4741 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
     4801 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga
     4861 ttgccgggca tcattatgct gctggtcacc ccgctggtaa tttacaccat gtatccacca
     4921 gaaattaaaa aggtggataa caaaaccatc gctaaagcgg gccttgccga actgggaccg
     4981 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
     5041 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
     5101 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
     5161 ttaatctggt acggcggtat tatcggctta agctccttat tatcgaaagt taaatttttc
     5221 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
     5281 ttcttcgtta ttattttcct cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc
     5341 tatatcgttg ctatgttacc tgtatttgcc atgctggcga acgtctccgg cgcaccgtta
     5401 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
     5461 ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa atcctggtgg
     5521 ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct cggcgtgtgg
     5581 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata cttcaagttg
     5641 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt
     5701 cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt
     5761 ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag
     5821 gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta
     5881 aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct cataatacgt
     5941 ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct
     6001 ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg ctgccgtatg
     6061 tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac ggtgatgacc
     6121 atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct ccgcatattg
     6181 cggtcatcca tcaatatatg cgtgagatga tggctagcgg gggcaaaatg atcctcgggt
     6241 cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag ggtggcggtg
     6301 agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg
     6361 tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg ctggctatca
     6421 ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg
     6481 gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca
     6541 cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg
     6601 gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca
     6661 tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg
     6721 tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg
     6781 agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg
     6841 gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg
     6901 tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg
     6961 cggtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag
     7021 atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc tttggcgcgg
     7081 gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc
     7141 gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt
     7201 ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct
     7261 gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg
     7321 gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc
     7381 cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg
     7441 aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc
     7501 cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga
     7561 ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg gtctatgcgg
     7621 tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg
     7681 gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac gtcatcaact
     7741 gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg gattacattt
     7801 acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga
     7861 tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg acggctgaag
     7921 agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa
     7981 aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat
     8041 cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc
     8101 ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg cccacgccgc
     8161 ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt
     8221 cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc accgcatcgg
     8281 cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac
     8341 cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca tcaccggaag
     8401 cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc gtagccggtg
     8461 caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg gtgttatcga
     8521 acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag ctgttggtca
     8581 ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg ttggtgacaa
     8641 agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacttgg tcaccgtcag
     8701 tacgcagtgc taccgccgga tggttacctg catctacgct atcgcccagc gtgttttcga
     8761 tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca gaacacagaa
     8821 caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac
     8881 ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc
     8941 catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtga
     9001 ttccacctgg agcagcaggg acatacaccg ttccctgata ctccccaggc atcacggcaa
     9061 tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg
     9121 tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg
     9181 ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgcg
     9241 catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag
     9301 tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa
     9361 aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat
     9421 gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc attataaaaa
     9481 agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat aaaatcatta
     9541 tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt
     9601 gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag
     9661 tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga aggaaagacc
     9721 tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg
     9781 acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc
     9841 cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc
     9901 tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact tcatttcgca
     9961 taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca
    10021 tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct
    10081 ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc
    10141 agctattgcc tctcggaatg catcgctcag tgttga
//
LOCUS       DAJTNW010000118        10114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNW010000118 DAJTNW010000000
VERSION     DAJTNW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..177)
                     /locus_tag="OO179_003500"
     CDS             complement(<1..177)
                     /locus_tag="OO179_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5533430.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="OO179_003501"
     CDS             complement(380..847)
                     /locus_tag="OO179_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5533431.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="OO179_003502"
     CDS             complement(844..1377)
                     /locus_tag="OO179_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5533432.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="OO179_003503"
     CDS             complement(1443..2072)
                     /locus_tag="OO179_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533433.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="OO179_003504"
     CDS             complement(2076..2291)
                     /locus_tag="OO179_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5533434.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2584..2658)
                     /locus_tag="OO179_003505"
     tRNA            complement(2584..2658)
                     /locus_tag="OO179_003505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Gly,seq:tcc)
     gene            complement(2662..2737)
                     /locus_tag="OO179_003506"
     tRNA            complement(2662..2737)
                     /locus_tag="OO179_003506"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2702..2704),aa:Thr,seq:tgt)
     gene            complement(2740..2816)
                     /locus_tag="OO179_003507"
     tRNA            complement(2740..2816)
                     /locus_tag="OO179_003507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2780..2782),aa:Arg,seq:tct)
     gene            complement(2818..2894)
                     /locus_tag="OO179_003508"
     tRNA            complement(2818..2894)
                     /locus_tag="OO179_003508"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Ile,seq:cat)
     gene            complement(3094..3474)
                     /locus_tag="OO179_003509"
     CDS             complement(3094..3474)
                     /locus_tag="OO179_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5533435.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3467..3667)
                     /locus_tag="OO179_003510"
     CDS             complement(3467..3667)
                     /locus_tag="OO179_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5533436.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3762..4019)
                     /locus_tag="OO179_003511"
     CDS             complement(3762..4019)
                     /locus_tag="OO179_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533437.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4016..5407)
                     /locus_tag="OO179_003512"
     CDS             complement(4016..5407)
                     /locus_tag="OO179_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5533438.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5404..6282)
                     /locus_tag="OO179_003513"
     CDS             complement(5404..6282)
                     /locus_tag="OO179_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5533439.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6293..7201)
                     /locus_tag="OO179_003514"
     CDS             complement(6293..7201)
                     /locus_tag="OO179_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5533440.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7188..7421)
                     /locus_tag="OO179_003515"
     CDS             complement(7188..7421)
                     /locus_tag="OO179_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533441.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7418..8434)
                     /locus_tag="OO179_003516"
                     /pseudo
     CDS             complement(7418..8434)
                     /locus_tag="OO179_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8366..8629
                     /locus_tag="OO179_003517"
     CDS             8366..8629
                     /locus_tag="OO179_003517"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5533442.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8619..8927
                     /locus_tag="OO179_003518"
     CDS             8619..8927
                     /locus_tag="OO179_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5533443.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8969..9145)
                     /locus_tag="OO179_003519"
     CDS             complement(8969..9145)
                     /locus_tag="OO179_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5533444.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9142..9552)
                     /locus_tag="OO179_003520"
     CDS             complement(9142..9552)
                     /locus_tag="OO179_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5533445.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9549..9827)
                     /locus_tag="OO179_003521"
     CDS             complement(9549..9827)
                     /locus_tag="OO179_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533446.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9902..>10114)
                     /locus_tag="OO179_003522"
     CDS             complement(9902..>10114)
                     /locus_tag="OO179_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533447.1"
                     /translation="QIQCATSPASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESG
                     SPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2446 a         2710 c         2321 g         2637 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc ctcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg caccgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagtctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     2521 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     2581 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     2641 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     2701 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     2761 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     2821 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac
     2881 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     2941 gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg
     3001 cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac
     3061 gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat
     3121 ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag
     3181 cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat
     3241 cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg
     3301 ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac
     3361 ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg
     3421 attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt
     3481 ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt
     3541 tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg
     3601 aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga
     3661 cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata
     3721 atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc
     3781 accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     3841 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     3901 tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc
     3961 atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa
     4021 agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg
     4081 gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt
     4141 tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt
     4201 ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa
     4261 tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt
     4321 tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac
     4381 cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc
     4441 ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat
     4501 atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt
     4561 ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt
     4621 cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag
     4681 acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat
     4741 gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag
     4801 gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt
     4861 catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac
     4921 gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg
     4981 tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg
     5041 tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg
     5101 cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac
     5161 gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt
     5221 caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc
     5281 gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc
     5341 cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag
     5401 ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca
     5461 gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc
     5521 ttccggtgag aggttactga taagaatggt gggcaagata ctttcgtacc tggcgttgat
     5581 aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat
     5641 gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc
     5701 attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact
     5761 ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt
     5821 accacaactg ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc
     5881 cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt
     5941 atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc
     6001 cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca
     6061 gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg
     6121 atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg
     6181 ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc
     6241 ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg
     6301 tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat
     6361 cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag tcttcgaagt
     6421 gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg gtatttcctg
     6481 tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca ggttttattc
     6541 cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta ccaccagcac
     6601 gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt cgggaagaac
     6661 gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct
     6721 ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc
     6781 gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag
     6841 tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat
     6901 taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa
     6961 ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc
     7021 cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat
     7081 cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa
     7141 cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca gacggacgca
     7201 tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag cggataactc
     7261 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt actgaccagg
     7321 caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     7381 ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc tccttgtcag
     7441 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     7501 acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc tggcttgtcc
     7561 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt gcgacagatg
     7621 acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag gcattgatgt
     7681 atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg aaggcgaagc
     7741 cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc tggcgtacca
     7801 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     7861 tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc agggatgaag
     7921 taacagccta accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     7981 tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca atcacagagg
     8041 tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca tcttgcgatg
     8101 tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa aaaaccgcat
     8161 gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc gctttataag
     8221 gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca gatataacga
     8281 ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag aggtccgact
     8341 ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg ttgaacgcat
     8401 gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg acgggctttt
     8461 cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca ctctgacagg
     8521 gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac cgccagccga
     8581 acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa cgcactctcc
     8641 ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct tttaaagcag
     8701 aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca gcacgcaaag
     8761 aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg accgcgatca
     8821 ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc acactaaaaa
     8881 aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca caccgcgccg
     8941 tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt agccaccgga
     9001 tatcccacag gtgagtcgtg tagttgaagg tttttacgtc agattctttt gggattggct
     9061 tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg
     9121 tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga caccctgacc
     9181 aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc tcatctgcgc
     9241 gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat cagcttcctg
     9301 ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg gttttaacca
     9361 tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa attttgcgtt
     9421 ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt ctttatcgag
     9481 aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc ggaggaagat
     9541 tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc tttcattact
     9601 gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc ttcttcacca
     9661 tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat agctgctcgc
     9721 gatgcctgcc atatcaccca ggcaaactct tttaattcat cgtctcctgt gaactggctt
     9781 ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc cctctccccc
     9841 aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg tagatggtca
     9901 tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat ttcggcatat
     9961 tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc gattgctcgt
    10021 caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt tcaagcatca
    10081 aaacagatgc cggtgaagtg gcacactgaa tttg
//
LOCUS       DAJTNW010000119         9952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNW010000119 DAJTNW010000000
VERSION     DAJTNW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO179_003523"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO179_003523"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5533448.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO179_003524"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO179_003524"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5533449.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO179_003525"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO179_003525"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5533450.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO179_003526"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO179_003526"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5533451.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OO179_003527"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OO179_003527"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5533452.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OO179_003528"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OO179_003528"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5533453.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OO179_003529"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OO179_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OO179_003530"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OO179_003530"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5533454.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OO179_003531"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OO179_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5533455.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9952)
                     /locus_tag="OO179_003532"
     CDS             complement(8974..>9952)
                     /locus_tag="OO179_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5533456.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2507 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tg
//
LOCUS       DAJTNW010000120         9929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNW010000120 DAJTNW010000000
VERSION     DAJTNW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..338
                     /locus_tag="OO179_003533"
     CDS             <1..338
                     /locus_tag="OO179_003533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533457.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="OO179_003534"
     CDS             526..1641
                     /locus_tag="OO179_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533458.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="OO179_003535"
     CDS             2320..2538
                     /locus_tag="OO179_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5533459.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="OO179_003536"
     CDS             2756..2998
                     /locus_tag="OO179_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533460.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OO179_003537"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OO179_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5533461.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OO179_003538"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OO179_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5533462.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OO179_003539"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OO179_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5533463.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OO179_003540"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OO179_003540"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5533464.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="OO179_003541"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="OO179_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5533465.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2544 g         2499 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaagaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattg caggacttat tcatttcgt
//
LOCUS       DAJTNW010000121         9905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNW010000121 DAJTNW010000000
VERSION     DAJTNW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO179_003542"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO179_003542"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5533466.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO179_003543"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO179_003543"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5533467.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO179_003544"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO179_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5533468.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="OO179_003545"
     CDS             complement(4692..6452)
                     /locus_tag="OO179_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR5533469.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="OO179_003546"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="OO179_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="OO179_003547"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="OO179_003547"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5533470.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="OO179_003548"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="OO179_003548"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5533471.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2482 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacggc gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAJTNW010000122         9641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNW010000122 DAJTNW010000000
VERSION     DAJTNW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(59..379)
                     /gene="psiF"
                     /locus_tag="OO179_003549"
     CDS             complement(59..379)
                     /gene="psiF"
                     /locus_tag="OO179_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5533472.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(498..1913)
                     /gene="phoA"
                     /locus_tag="OO179_003550"
     CDS             complement(498..1913)
                     /gene="phoA"
                     /locus_tag="OO179_003550"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5533473.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2014..2274)
                     /gene="iraP"
                     /locus_tag="OO179_003551"
     CDS             complement(2014..2274)
                     /gene="iraP"
                     /locus_tag="OO179_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5533474.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2456..2659)
                     /locus_tag="OO179_003552"
     CDS             complement(2456..2659)
                     /locus_tag="OO179_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533475.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2737..3831
                     /gene="ddlA"
                     /locus_tag="OO179_003553"
     CDS             2737..3831
                     /gene="ddlA"
                     /locus_tag="OO179_003553"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5533476.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3855..4067)
                     /locus_tag="OO179_003554"
     CDS             complement(3855..4067)
                     /locus_tag="OO179_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5533477.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4327..4635
                     /locus_tag="OO179_003555"
     CDS             4327..4635
                     /locus_tag="OO179_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5533478.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4694..5788)
                     /gene="yaiW"
                     /locus_tag="OO179_003556"
     CDS             complement(4694..5788)
                     /gene="yaiW"
                     /locus_tag="OO179_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5533479.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5801..7021)
                     /gene="sbmA"
                     /locus_tag="OO179_003557"
     CDS             complement(5801..7021)
                     /gene="sbmA"
                     /locus_tag="OO179_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5533480.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7373..8530
                     /gene="ampH"
                     /locus_tag="OO179_003558"
     CDS             7373..8530
                     /gene="ampH"
                     /locus_tag="OO179_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5533481.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8531..9034)
                     /locus_tag="OO179_003559"
     CDS             complement(8531..9034)
                     /locus_tag="OO179_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5533482.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9122..>9641)
                     /locus_tag="OO179_003560"
     CDS             complement(9122..>9641)
                     /locus_tag="OO179_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5533483.1"
                     /translation="YSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDAGY
                     VTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPYFK
                     LAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQ
                     DWSANVGVKYTW"
BASE COUNT         2396 a         2460 c         2294 g         2491 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca atcaggtatc
       61 aggcggcttt cttgaggcag gcactcataa acttattacg atcatcacct ttcagagctt
      121 gttgcgttgc ttgattatta cattcacgca tcttttgctg ctgtggcgtc aaactttttt
      181 caccaggcgc agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc
      241 ctttcaacgc ctgcgccgtc gcctgctgat tacaggaggt catacgctgt tgttgtgggg
      301 ttaaggttct ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa
      361 ccagtaatgt tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtttgcgc
      421 ccgtgatctg ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt
      481 cactgccggg cgcagtttta tttcagcccc agagcggctt tcatggtgta gaagagatcg
      541 gtctggtcgg tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac
      601 tgactgccgg tatgttcttg tgaatcctct tcggaattcc cgtaactcat caccatcact
      661 gcgccatctt tagtatttag cgcctgagtg aggcccggcg ctttggtatc cggcgcaaca
      721 acctggctgg cgtgggcgtg atcagcggtg actatgacca gcgtgttacc ctccttttta
      781 gcgaattcca gcgcccgttg tacggcttca tcgagatcga ccgtctcgcc aatttgccca
      841 caagggttcg cagcgtgatc ctgtttatcg attgacgcac cttcaacttg caggaaaaat
      901 cctttctcat ttttactcaa caattcaatg gctttgtcgg tcatctgtgc cagggttggt
      961 acactgtcat tacgttgcgg attaggcgta taggtgactg cgggcttatc gatattgccg
     1021 tggtacgttg ctttcggccc tagccagcgc actggcatat tgccgtcagc aaacaggcca
     1081 agcaggggtt tttgctgatt cgcttccgtc accgaattca gtgaggcagc atcgttcacc
     1141 aactgataac cacgcgcctg tgcctgttca cgcagcgttt ttccctgcca ttcaccagcg
     1201 gttgccgttt cagcaaaggt ttttgcgccg ccgccaagcg taacgtcggc acgagcgtta
     1261 agcagctgtt cggtaatcga tccttttccg cctttttcca gagcgttacc cggacatttt
     1321 tcactggtcg cgctcggacc gtagcatttg cgcgaagtca catgtgccac cagcgcagcg
     1381 ggcgtggcat cctgcaactc tgcggtagaa acgttaccgg tcgccagacc tgcggctttt
     1441 gccatttcca gaatcgttgg gtgatctttt tcgtgaatat cgacgcccag cgcgccgtta
     1501 taggttttga caccggttga ccaggcggtt gctgatgcag ccgagtcggt gacgtagtcc
     1561 ggtttgccag tttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca
     1621 tcgatacctt taaaaaagcc gcccgcacct tcggcataat tacgtgcggc agtaatttcc
     1681 gaatccccca tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga
     1741 gaatcacgca gagcggcggt ctgatcaccc gttaagcggc gagcaccgcc gggtgcagta
     1801 atatcgccct gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc
     1861 acaggggtaa acagtaacgg taagagtgcc agtgcaatag tgctttgttt cactttattt
     1921 tctccatgta caaatacatt aaaaaataaa aacaaagcga ctataagtct cggccgtgac
     1981 aactttatga cagctgttga aaagattaac tttttactga caaggatgct tcaataactt
     2041 ctttacgtaa tcgcgcagca gctctgtatc gtcgtcagga atgctggcat cgggctttac
     2101 ctcgtacagc gccccctcta cctgatcaat caaccgctgt tggtcatttt gcgccatatt
     2161 gcgaagcatt gcagtgacga taatctccaa agcttctacc tgcgcacaca gttctttcga
     2221 ctcttcttct ttttgggcaa gcttaaataa caactcagca atgagatttt tcatgtctgt
     2281 atttccttat ccaaagtatg gagaagttaa cattatgttt cacaacttca tagtgtctgg
     2341 aagtattatt tttatgacaa aggaaatact ttgcgcaaat attttttgtt tgattaccag
     2401 cgaaacgttt ctcttattta aataattacc cacacaaaat ataggctttt aattatcaac
     2461 ctttattata aataaaagct atgaaatata ttctcttcgt taacttataa ttaagagaaa
     2521 aaaactcacc gcattgccat ttaagccagg aataaattag cttcccgttc atcatttttg
     2581 tgatcttgcg cacatctttg agataacgca ctttcttcac cagtacgttg cacgctttgt
     2641 cagcagaaga ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat
     2701 gtatgcactt tgtgttagac gggataggtt tttaagatgg aaaaactgcg ggtaggaatc
     2761 gtttttggtg gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc
     2821 gatgccattg ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa acaagggcaa
     2881 tggcacgtca gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg
     2941 ttgcgccctt cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc
     3001 gacgcgcaaa acggtcagcc gttgccgacg gtggatgtca ttttcccgat tgtccacggt
     3061 acgctgggcg aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta
     3121 ggttctgatg ttctggcttc agcagcctgt atggataaag atgtcaccaa acgtctgcta
     3181 cgcgatgccg ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac
     3241 atcagttttg ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa accggctaat
     3301 cagggctctt ctgttggtgt cagcaaagta accagtgaag aacagtacgc aattgccgtc
     3361 gatctggcgt tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgag
     3421 atcgaatgcg cagttctggg caacgacaat ccgcaagcca gcacctgtgg cgagatcgta
     3481 ctcaccagcg atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg
     3541 gtagttccgg cagccattgc accagagatc aacgataaga tccgggcgat tgccgttcag
     3601 gcttatcaga cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag
     3661 aacgaagtgg tgatcaacga gatcaacaca ctgcctggct tcaccaacat cagtatgtat
     3721 ccgaagctgt ggcaagccag cggtctgggt tacaccgatc tgatcacacg tttgattgag
     3781 ctggcgctgg agcgtcacgc tgcggataac gcattgaaaa ccacaatgta atattttaac
     3841 gcccgataat attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcaac
     3901 cccgccagcc agaaactaat cacccacgtc accagaccga cggcataggt ctgccaacct
     3961 tttgcttcaa atcccagtac ccccaccacg ccattaagaa tgaatataag gccaatggcg
     4021 aacgcatagt agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta
     4081 acgaaaaaca acatccttgc atattctgta acaaccgtct gtggtgtaga caacattttc
     4141 gtaattatta ataaatttaa cttaaggttg ccattttgtg actcacaacg aagattcacg
     4201 cttcgggaat attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc
     4261 tgtcagactt gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag
     4321 gtttttatgg ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc
     4381 tcttcaacgc ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg
     4441 gcgcaattgc tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc
     4501 caggaaacag gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg
     4561 atcccgtttt tagcaggctg cctcattttt gcagtggtgg cgctatggct gcactggcga
     4621 catcgccgcc agtaaaattt acgttgccag atgcggcctg aaacgcctta tctggcctga
     4681 cattcctgtt tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa
     4741 accaggccgt agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca
     4801 tttcgcgggg tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca
     4861 gcgccgtctc ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt
     4921 cgctcatccc cagttttccc gccagtttgc gcgttgccag ttcggttttc ctgggttctt
     4981 tactgtcgta acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga
     5041 ctgcgttctg aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc
     5101 ggtatatcgg tgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca
     5161 acccgccgcg gcgactgaag acttcctgac gaactgtacc gtccattttc cacggatacc
     5221 ctttggtatg ctgttcggca aaagcaatgc tgacctgcat cggcccaccg gtgcgcaccg
     5281 gattaagcga accaaacagc gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa
     5341 tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg ttcgctataa cttttgccgt
     5401 ttggcgattt gatttttagc gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag
     5461 cacgacggtc aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct
     5521 gataatttga ctcctgctgc gccaccgcca gcaccgagta gacgttttcc agcgtcggtg
     5581 ccagcccctg actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca
     5641 cgctcgcagg catcttctgc cgcacgacgc tcgctacatc tatcgctttt tcgccctttt
     5701 tcaacggttg cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca
     5761 gcgaagatag atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta
     5821 cttcctgaat tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag
     5881 acatcaactc aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct
     5941 gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg
     6001 acggaaacag caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga
     6061 tgttgaaata catatagtga aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca
     6121 gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt
     6181 tacggtaggc ggcctctact cgctggtttt taaactccag ccccggcagt ttgatcccta
     6241 ccactgccag caatccggtc cccatcagcg accagacgat agcggcaatc accagaccat
     6301 acggaatgtg cccgataatc ggcagctccg gcacatgcgc ggagagcgtt accagcaccg
     6361 gcaggaaggc gatcaacgtc attatggcgt tgataaaact gacgcccata ttctccagcg
     6421 ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca
     6481 gttgttgcca gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacgtagt
     6541 gactgacaaa gaagttgttc agtacactga tcaccactgc gatcagcgca atccccagaa
     6601 agacgcccac ttcgcggtaa aattgttcga tagtgacttt atgcggcgaa cttagcgcgg
     6661 tttgaatcag atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca
     6721 aaaaccaagt gacgaagata atcagcgcag taccgagtat tgaccagtat tgccaacggt
     6781 gcgggctgta gataaaccag aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt
     6841 aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag
     6901 cgccggtgat atgtgccacc cagtcacccc caccggcttg ccagaagata acggcaatca
     6961 atgcccaaac aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca
     7021 tatgcactcc tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg
     7081 ctacaaagcg tcagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg
     7141 ccagcctgac cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag
     7201 cggattaaca cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt
     7261 tacgcgccgt tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa
     7321 tgagtagtat aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg
     7381 tagtctgctt ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca
     7441 gccgatcact gaaccggagt ttgcctctga tattgtcgat cgttatgccg atcatatttt
     7501 ttacggcagc ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt
     7561 tcgtagttat ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg attcggtcgt
     7621 gcgtattgct tccctcacca aactaatgac cagtgaaatg ctggtgaaat tgctcgacca
     7681 ggggaccgta aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtgcc
     7741 gacctacaat gggacgccca ttaccctagt gaatctggca acccatacca gcgccctgcc
     7801 ccgtgaacag cccggtggcg cggcacatcg tccggtgttt gtctggccaa cgcgcgagca
     7861 acgctggaaa tacctttcta cggcgaagct gaaagccgcg ccaggcagtc aggcggcgta
     7921 ctctaacctt gcgttcgacc tgctggcaga tgcactggcg aatgcgtccg gcaagcctta
     7981 tacccagttg tttgaagagc agattaccca tccattgggg atgaaagaca ccacctacac
     8041 cccctcaccg gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa
     8101 taacacgctg gcggcaattg gcagcggcgg cgtctattcc acgcctggcg atatgatgcg
     8161 ctggatgcag cagtatttgt catctgattt ttatcagcgc agtaaccagg cagatcgcat
     8221 gcagacgttg atttatcagc gcgcgcagtt tacgaaagtg attggcatgg atgtccccgg
     8281 caaagcggat gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccgag
     8341 gattattcag aagacaggcg gtggtggcgg tttcattacc tatatggcga tgatcccgca
     8401 gaagaatatt ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat
     8461 gagcgatggc atcaatgatc tggtgaccga gctaagcggg aataaaccgt tggttatccc
     8521 cgcatcctga ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca
     8581 tttcgatata gccgccttta cgcaaagcag cgagaacttc tgcgacgaca gaacgcgata
     8641 tgcgtgtacg ttgatggata taattcatca caccaatacg tgatcgcaat tcttcattcc
     8701 agtcaatcat tgataataat gttgcgcggg tttgtttggc tggtagatga tatcccgtac
     8761 aatttccttc tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagcc
     8821 ccataatata aggagcctgg gtaataccga taagtacgtt ttcttctcta cgcagagaga
     8881 taactccttc taaaataacg aaagtatctt cgttattgac atcactggaa aatataactt
     8941 gcttttcatt attaaactcg aagcgcgtac cgtatctgga caaacattta tcgagcttac
     9001 caaattcctg aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg
     9061 tcaaatcctt gacgggcatt atttagatta aattaccagt atttcttcgg agtgaagaat
     9121 attaccaagt atatttaaca cccacgttcg cggaccagtc ttgatctacg tcaccaccac
     9181 cgaggtagtt agcatcggta taggcgctga agttcttggt gaagctgaac tgagtaccca
     9241 gaccaacacg taccgcagac ccttcagtac cgttatcgat ggaatcaccg ttcacatcgt
     9301 tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc agagcctgat
     9361 cttcgctgta ggtgaaggta taacctgcat ctacacccag ttcataacgc atgctgtcgt
     9421 aaggctgacc gtcaactttc atgtcgttgc tcagctggta gtcatcacca gactggaaca
     9481 gaccagaaat gctgccgtaa ggagtcacgt aaccagcatc acccagtttg aagtcgtaac
     9541 cggttttcaa accgaagccc caagcgtcgg agttggtgct accgtcaacg taagtaccgt
     9601 tgctcatggt tgcagacagg tcgttgttga agtgagagta g
//
LOCUS       DAJTNW010000123         9628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNW010000123 DAJTNW010000000
VERSION     DAJTNW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(18..1001)
                     /gene="kdgT"
                     /locus_tag="OO179_003561"
     CDS             complement(18..1001)
                     /gene="kdgT"
                     /locus_tag="OO179_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5533484.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1261..1881)
                     /gene="sodA"
                     /locus_tag="OO179_003562"
     CDS             complement(1261..1881)
                     /gene="sodA"
                     /locus_tag="OO179_003562"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5533485.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2166..3200
                     /gene="rhaT"
                     /locus_tag="OO179_003563"
                     /pseudo
     CDS             2166..3200
                     /gene="rhaT"
                     /locus_tag="OO179_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3197..4063)
                     /gene="rhaR"
                     /locus_tag="OO179_003564"
     CDS             complement(3197..4063)
                     /gene="rhaR"
                     /locus_tag="OO179_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5533486.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4119..4955)
                     /gene="rhaS"
                     /locus_tag="OO179_003565"
     CDS             complement(4119..4955)
                     /gene="rhaS"
                     /locus_tag="OO179_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5533487.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5243..6712
                     /gene="rhaB"
                     /locus_tag="OO179_003566"
     CDS             5243..6712
                     /gene="rhaB"
                     /locus_tag="OO179_003566"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5533488.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6709..7968
                     /gene="rhaA"
                     /locus_tag="OO179_003567"
     CDS             6709..7968
                     /gene="rhaA"
                     /locus_tag="OO179_003567"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5533489.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8210..9034
                     /gene="rhaD"
                     /locus_tag="OO179_003568"
                     /pseudo
     CDS             8210..9034
                     /gene="rhaD"
                     /locus_tag="OO179_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9044..9358
                     /gene="rhaM"
                     /locus_tag="OO179_003569"
     CDS             9044..9358
                     /gene="rhaM"
                     /locus_tag="OO179_003569"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5533490.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2491 g         2270 t
ORIGIN      
        1 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
       61 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
      121 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
      181 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
      241 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
      301 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
      361 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa
      421 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
      481 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
      541 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
      601 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
      661 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
      721 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
      781 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
      841 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
      901 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
      961 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
     1021 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
     1081 agacattcat ttaatcaata aaagtgatca aaatcacatc aattgaaacg ctgttttatt
     1141 tttataagac cattgttttt atttttaaat tgatcacaaa aaaaccaccg ccgttggcga
     1201 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
     1261 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
     1321 tttgatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
     1381 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
     1441 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
     1501 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
     1561 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
     1621 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
     1681 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
     1741 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
     1801 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
     1861 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
     1921 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
     1981 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
     2041 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
     2101 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
     2161 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
     2221 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
     2281 cagtcggtgg gattgtttcg tggattattc tgccgtgggc catcagcgcc ctgttactac
     2341 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
     2401 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
     2461 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
     2521 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttac
     2581 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
     2641 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
     2701 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
     2761 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt tgctctgcca agctatgttg
     2821 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
     2881 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
     2941 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctagg
     3001 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
     3061 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
     3121 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
     3181 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
     3241 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
     3301 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
     3361 tgacctgccg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
     3421 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
     3481 ggctagccgc cagccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
     3541 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
     3601 cagccatttc gttagcaaac ggcacatgct gactactttc atgctcaagc tgaccgataa
     3661 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
     3721 cgctaaatcc cggaatcgct ccctgccagt caagattcag cttcagccgc tccgggcaat
     3781 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
     3841 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
     3901 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
     3961 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
     4021 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
     4081 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
     4141 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
     4201 ttactgtcgc tgaatccaca gcgataggcg atgtcagtga cgctggcctc gctgtggcgt
     4261 agtagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
     4321 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
     4381 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
     4441 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
     4501 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
     4561 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
     4621 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
     4681 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
     4741 agatgccgat catgatcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
     4801 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
     4861 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacaa
     4921 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
     4981 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaactacgta
     5041 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
     5101 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
     5161 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
     5221 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
     5281 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
     5341 cgaaatccat cgttttaaca atgggctgca tagccaaaac ggttatgtca cctgggatgt
     5401 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
     5461 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
     5521 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc accaatggcc tgatgccgca
     5581 ggcacaacag caactcggca aacgcgatat ttatcagcgt agcggcatcc agtttctacc
     5641 cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa caacctgaac ttattccaca
     5701 cattgctcac gctctgctga tgccggatta cttcagttat cgcctgaccg gcaagatgaa
     5761 ctgggaatat accaatgcca ccaccacaca actggtcaat atcaatagcg acgactggga
     5821 cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc
     5881 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
     5941 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc
     6001 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
     6061 tgacacggcg ctggcagcca acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt
     6121 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa
     6181 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
     6241 tcccaatgac gatcgcttta ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg
     6301 tcgggaaacg gcgcaaccga tcccgggaag tgatgctgaa ctggcgcgct gtattttcga
     6361 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga
     6421 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
     6481 cgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa
     6541 tatcggcatc gagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
     6601 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
     6661 ctatgtggcg cagattcact ctacacgaca gacaaaggag ctttgcgcat gaccactcaa
     6721 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
     6781 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
     6841 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccgggg ggattcaggc cacaggcaat
     6901 tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg atctggaaca ggctatgcgg
     6961 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agatacgcca
     7021 gtctcgcgcg accagatcaa accagagcac ttcaaaaact gggttgaatg ggcgaaagcc
     7081 aatcagctcg gtctggattt taacccctcc tgcttttcgc atccgctaag cgccgatggc
     7141 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
     7201 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
     7261 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
     7321 ctggcagcac tggatgaggt gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc
     7381 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
     7441 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcacttccac
     7501 ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
     7561 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tagtgctgct ggatgatgaa
     7621 acccaggcaa ttgccagtga gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc
     7681 cttgacttct tcgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat
     7741 atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa
     7801 gcggcgggcg attacactgc gcgtctggca ctgctggaag agcagaaatc gttgccgcgg
     7861 caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg
     7921 gagagcgtgc gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat
     7981 gcggcacgag cgccttatcc ggcctacggg tcgaatgtcc atgcgggctg tcaccctcac
     8041 cccggccctc tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct
     8101 gaacaccgct ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca
     8161 agccgcacca tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat
     8221 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct agctgaaagg
     8281 ctgggacgag cgcaacggcg gcaacctgac gctgcgcctg gatgacgccg atatcgcgct
     8341 ttatcacgac aacttccacc cacaaccgcg ctatattccg ctcagccaac ccatgccttt
     8401 actggcaaat acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
     8461 cgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat
     8521 tctctggggg ttaaccaacg aagccgttcc aacttctgaa cttccggctc actttctctc
     8581 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
     8641 caccaacctg atcgccctca cctatgtact ggaaaacgac accgcggtct ttactcgcca
     8701 actgtgggaa ggcagcaccg aatgtctggt agtattcccg gatggcgttg gcattctgcc
     8761 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
     8821 ttcgctggtg ttgtggccct tccacggtgt gttcggttcc ggaccgacgc tggatgaagc
     8881 ctttggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
     8941 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
     9001 cgttacacca ctagccagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
     9061 tgtcatgcag gtaaaccccg acgcccacga agagtatcag agtcggcata atcccatctg
     9121 gccggaactg gaagcagtgt tgaaatctca cggtgtgcat aactacgcca tttacctcga
     9181 caaagcgcgt aatctgctgt ttgccatggt agagattgaa tctgaagaac ggtggaatgc
     9241 ggtagccagt actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
     9301 taacccagat aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt
     9361 gcccaatgtg ccggagatgc ctccggcaca atattttaaa tttcgatttg aaaaattaaa
     9421 catattacat ctcccggtaa gttatatttc cctgatacat tgtgagtaaa tcacaaaaat
     9481 aatgaataac ccattaatga ttcatgtggt ttatttaaat aaccattatg tgcattactc
     9541 cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc
     9601 atcacttaat cgctccacca ttacaatt
//
LOCUS       DAJTNW010000124         9483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNW010000124 DAJTNW010000000
VERSION     DAJTNW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..190)
                     /locus_tag="OO179_003570"
     CDS             complement(<1..190)
                     /locus_tag="OO179_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5533491.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO179_003571"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO179_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5533492.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO179_003572"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO179_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5533493.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO179_003573"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO179_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5533494.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO179_003574"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO179_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO179_003575"
     CDS             complement(3262..3381)
                     /locus_tag="OO179_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5533495.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO179_003576"
     CDS             complement(3342..3527)
                     /locus_tag="OO179_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533496.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO179_003577"
     CDS             3628..3801
                     /locus_tag="OO179_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533497.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO179_003578"
     CDS             3803..4147
                     /locus_tag="OO179_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5533498.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO179_003579"
     CDS             4151..4486
                     /locus_tag="OO179_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5533499.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO179_003580"
     CDS             complement(4543..6864)
                     /locus_tag="OO179_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5533500.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO179_003581"
     CDS             complement(6981..7190)
                     /locus_tag="OO179_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533501.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO179_003582"
     CDS             complement(7941..9464)
                     /locus_tag="OO179_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5533502.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2655 a         2202 c         2079 g         2547 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttgc tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aag
//
LOCUS       DAJTNW010000125         9399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNW010000125 DAJTNW010000000
VERSION     DAJTNW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO179_003583"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO179_003583"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5533503.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO179_003584"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO179_003584"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5533504.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO179_003585"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO179_003585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5533505.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO179_003586"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO179_003586"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5533506.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO179_003587"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO179_003587"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5533507.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO179_003588"
     CDS             5060..6193
                     /locus_tag="OO179_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5533508.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO179_003589"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO179_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5533509.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO179_003590"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO179_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5533510.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO179_003591"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO179_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5533511.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO179_003592"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO179_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5533512.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO179_003593"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO179_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5533513.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO179_003594"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO179_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5533514.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2377 a         2422 c         2416 g         2184 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaaca
//
LOCUS       DAJTNW010000126         9326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNW010000126 DAJTNW010000000
VERSION     DAJTNW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..923
                     /locus_tag="OO179_003595"
     CDS             <1..923
                     /locus_tag="OO179_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5533515.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO179_003596"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO179_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5533516.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO179_003597"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO179_003597"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5533517.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO179_003598"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO179_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5533518.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO179_003599"
     CDS             4446..4805
                     /locus_tag="OO179_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5533519.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OO179_003600"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OO179_003600"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5533520.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="OO179_003601"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="OO179_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5533521.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="OO179_003602"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="OO179_003602"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5533522.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2156 a         2249 c         2502 g         2419 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgcagctgag aaaaagcgaa gcggcactgc
     9181 tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg
     9241 caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta cgaagtttaa
     9301 ttctttgagc atcaaacttt taaatt
//
LOCUS       DAJTNW010000127         9254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNW010000127 DAJTNW010000000
VERSION     DAJTNW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO179_003603"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO179_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5533523.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO179_003604"
     CDS             1828..3147
                     /locus_tag="OO179_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5533524.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO179_003605"
     CDS             3445..3486
                     /locus_tag="OO179_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533525.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO179_003606"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO179_003606"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5533526.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO179_003607"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO179_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO179_003608"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO179_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO179_003609"
     CDS             5815..6711
                     /locus_tag="OO179_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5533527.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OO179_003610"
     CDS             6708..7694
                     /locus_tag="OO179_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5533528.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OO179_003611"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OO179_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO179_003612"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO179_003612"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5533529.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2263 a         2260 c         2319 g         2412 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caag
//
LOCUS       DAJTNW010000128         9015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNW010000128 DAJTNW010000000
VERSION     DAJTNW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO179_003613"
     CDS             complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO179_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5533530.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO179_003614"
     CDS             complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO179_003614"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5533531.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO179_003615"
     CDS             complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO179_003615"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5533532.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO179_003616"
     CDS             complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO179_003616"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5533533.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4450..5574
                     /gene="dprA"
                     /locus_tag="OO179_003617"
     CDS             4450..5574
                     /gene="dprA"
                     /locus_tag="OO179_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5533534.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5546..6019
                     /locus_tag="OO179_003618"
     CDS             5546..6019
                     /locus_tag="OO179_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5533535.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6048..6590
                     /locus_tag="OO179_003619"
     CDS             6048..6590
                     /locus_tag="OO179_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5533536.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6595..7167
                     /gene="tsaC"
                     /locus_tag="OO179_003620"
     CDS             6595..7167
                     /gene="tsaC"
                     /locus_tag="OO179_003620"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5533537.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGIGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7172..7990
                     /gene="aroE"
                     /locus_tag="OO179_003621"
     CDS             7172..7990
                     /gene="aroE"
                     /locus_tag="OO179_003621"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5533538.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7987..8244
                     /locus_tag="OO179_003622"
     CDS             7987..8244
                     /locus_tag="OO179_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5533539.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8220..8774)
                     /locus_tag="OO179_003623"
     CDS             complement(8220..8774)
                     /locus_tag="OO179_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5533540.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2188 c         2246 g         2323 t
ORIGIN      
        1 tctaacaaat gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca
       61 aatggaaata ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggct
      121 ggaagagtct ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat
      181 cgccttgctc aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg
      241 ctccaataat cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg
      301 aggtgctttc atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg
      361 aggaaacacc aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt
      421 gttgtggtga aattgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac
      481 gctggatcaa taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga
      541 tattggcctc gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt
      601 cggccagcag ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt
      661 ccgccagttc ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt
      721 ccagacgacg cgccagccct gcaccgatat taccgccacc aaccagcatg atccgtttat
      781 acggtttttc cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa
      841 taaagaacac ttcatcacca gcttcaacaa tggtcgaacc ttgtggacga atggggcgat
      901 cgtggcggaa aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg
      961 aaagtgcatt accaatcagc gggccgccat aataggcttt aactacagcg aggctgactt
     1021 taccctcagc gaagttcacc acctgcaatg cgcctggata ctcaatcagt cggtaaatat
     1081 tatcgataac caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa
     1141 atagcttatc ggcatcgcgt acgtagtctg gtgagcggat gcgagcgatg cgattagggg
     1201 tgttgaaaag tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta
     1261 cagcaaccag catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc
     1321 catgcccctg cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt
     1381 tggtatcgac aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc
     1441 caacctggcc ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact
     1501 ttttgattag cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc tgattttgtt
     1561 tacccggttg ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa
     1621 aggctttaat ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg
     1681 ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga
     1741 gttccgggat atcgcgatcg cgacgtaacc atttaatatc tggatggcga cgaatcaccc
     1801 ccgttgctga acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt
     1861 gggaagggta acggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat
     1921 tgtcgtaaat gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg
     1981 gtgccacctc aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaagatgt
     2041 gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg
     2101 taacccatcc gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta
     2161 cagcatcagg gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa
     2221 gccagctgtc gcgggaatga tgcgtacgat taatacgcag ccacattggc ggacgctggt
     2281 tattggcttc ggcgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca
     2341 accaggaagg atgcagataa cgtgcatcac gggtattaaa ctcggctaat aactcttctt
     2401 gccgacgctg gaactggcgt aatacaccgt taatcaaccc tttaagttgc ggacgcttaa
     2461 ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa
     2521 gcagttgata caaaccaacc ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg
     2581 gacgggccat taacttatta atcagccagt ctaactgcga aagcgtacgc agtacgccaa
     2641 agcacaactc ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca
     2701 gaatgttgct taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc
     2761 tacgtaaatt acgttgtttt ttcataagta taaaaatgct cggcaagacc gggcttagaa
     2821 gagtggacta tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga
     2881 ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc
     2941 catcaccagt cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag
     3001 cgttggttgc cgtatcaatg accgacgctt tccagacttt aaccggctgt ccttcaattt
     3061 ccagccagct cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg
     3121 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag
     3181 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa
     3241 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
     3301 taatcgggca ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca
     3361 taatggttac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac
     3421 cgcgccagcg tggcagcagt gaaccatgaa tgttgataca gccaagacgc ggcatctcca
     3481 gcacagcttt aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcaaat
     3541 cggcgaccag ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac
     3601 cttcttcctc agccagaact ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg
     3661 gtcggtctgg ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga
     3721 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
     3781 tagttcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
     3841 tcgttgttgt ttcagcggtg acagataatc cataaacagt ttgccaacca ggtgatccat
     3901 ctcatgctga atacagatag ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc
     3961 gcggtcaagg gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg
     4021 gatcgacagg caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
     4081 gattaacacc agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg
     4141 gatatcaacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
     4201 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
     4261 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
     4321 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
     4381 cacttgattg acagcatcac tgaccaatcg caaagattgc taaggcttgc ttatggcagg
     4441 gagataagga tggtcgatac agaaatttgg ctgcgtttaa tgagcatcag cagcttgtac
     4501 ggcgatgata tggtccgtat cgctcactgg ctggcaaaac agtcgcatat tgatgcggtt
     4561 gtattgcagc aaacagggct tacattgcgg caggcacaac gctttctttc atttccacga
     4621 aagagtatcg aaagctcact ttgttggttg gagcaaccca accatcattt aatccctgcg
     4681 gacagcgaat tttatcctcc tcaacttcag gcgacgacag attaccccgg cgcactgttt
     4741 gttgaaggag aactgcacgc gctgcattca tttcagcttg ccgtagtggg gagtcgggcg
     4801 cattcatggt atggcgagcg atgggggcga ttattttgcg aaactctggc gacgtgtgga
     4861 gtgacaatta cgagtggact ggcgcgtgga atcgatggtg tggtgcataa agcggcctta
     4921 caggtaaatg gcgtcagcat tgctgtattg gggaatggac ttaataccat tcatccgcgt
     4981 cgccatgccc gactggctgc cagtctgttt gaacagggtg gcgctctcgt ctcggaattt
     5041 cccctcgatg ttccacccct tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt
     5101 ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca gtggttcgct ggtgacagca
     5161 cgttgtgcgc ttgagcaggg gcgtgaagtt tttgccttgc caggtccaat agggaatccg
     5221 ggaagcgaag ggcctcactg gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa
     5281 gaaattctgg aaaacttgca atttggattg cactggttgc cagacgcccc tgaaaattca
     5341 ttttattcac cagatcagca agacgtggca ttgccatttc ctgagctcct ggctaacgta
     5401 ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag
     5461 gtagttactc aactactcga actggagtta gcaggatgga tcgcagctgt acccggcggc
     5521 tatgtccgat tgaggagggc atgccatgtt cgacgtacta atgtatttgt ttgaaaccta
     5581 tattcacact gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga
     5641 cgcaggtttt gagcgagaag atatctacaa tgccctgcta tggctggaaa aacttgctga
     5701 ttatcaggaa gggttggcag aaccgatgca actggcctcg gatcctctct ccatgcgtat
     5761 ttatacaccg gaagagtgtg aacgactgga tgccagctgc cgtgggtttc tgcttttcct
     5821 tgagcagatt caggtgctca accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc
     5881 gctggataac gcagagttcg aactggatga tctgaaatgg gtgatcctga tggtgttgtt
     5941 caacatcccg ggctgcgaaa atgcgtacca gcaaatggaa gaattactct ttgaagtgaa
     6001 tgaaggtatg ctgcattaat catttcttta attcagcata agttgttatg gcaaaatcag
     6061 cactgttcac ggtgcgtaat aatgagtcct gcccaaagtg cggggctgaa ctggttattc
     6121 gatccgggaa acacggtccg tttcttggat gctcacagta tccggcgtgt gactacgtcc
     6181 gtcctctgaa atcttcagcg gatggacata ttgtcaaagt tctggagggg caggtttgcc
     6241 ccgtatgtgg cgcaaatctg gtattacgcc agggacgctt tggtatgttt attggttgca
     6301 gtaactaccc tgaatgcgaa cataccgaac ttatcgataa accggacgaa acagcaatta
     6361 catgccccca atgtcggacg ggccatctgg tccagcgccg ctcccgttat ggcaaaacat
     6421 ttcactcctg tgatcgctac ccggagtgtc aatttgccat taacttcaaa ccaatagctg
     6481 gagaatgccc tgagtgtcat tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa
     6541 aacacttttg tgccagtaaa caatgtggaa agccggtttc ggcggaataa taacgtgaat
     6601 aataacctgc aaggagacgc tatcgcagct gcgatagatg tcctcaatga aaaacgtgtc
     6661 atcgcctatc caacggaagc cgttttcggt atcgggtgcg atcctgatag cgaaacagca
     6721 gtgatgagac tgttggagtt aaaacagcgt ccggttgata aggggctgat tttaatcgcg
     6781 gcaaattacg agcagcttaa accctatatt gatgacacca tgttgactga cgtgcagcgt
     6841 gaaaccattt tttcctgctg gccaggtcct gtcacctttg tctttcccgc gcctgcgaca
     6901 acaccgcgct ggttgacggg ccgctttgat tcgcttgctg tacgagtcac cgaccatccg
     6961 ttggtggttg ctttgtgcca ggcttatgat aaaccgctgg tttctaccag tgccaacttg
     7021 agtggattgc caccttgtcg aacagtagac gaagttcgcg cacaatttgg cgcggcgttc
     7081 ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg cgatgccctg
     7141 acgggtgaac tgtttcgaca ggggtaacat aatggaaacc tatgctgttt ttggtaatcc
     7201 gatagcccac agcaaatcgc cattcattca tcagcaattt gctcagcaac tgaatattga
     7261 acatccctat gggcgcgtgt tggcacccat caatgatttc atcaacacac tgaacgtttt
     7321 ctttcgtgct ggtggtaaag gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc
     7381 cagagcggat gagcttactg aacgggcagc gttggctggt gcagttaata ccctcaagcg
     7441 gttagaagat ggacgcctgc tgggtgacaa taccgatggt gtaggcttgt taagcgatct
     7501 ggaacgtctg tcttttatcc gccctggttt acgtattctg cttatcggcg ctggtggagc
     7561 atctcgcggc gtactactgc cacttctttc cctggactgt gcggtgacaa tcactaatcg
     7621 gacggtatcc cgcgcggaag agttggctaa attgtttgcg cacactggca gtattcaggc
     7681 gttgggtatg gacgaactgg aaggtcatga gtttgatctc attattaatg caacatccag
     7741 tggcatcagt ggtgatattc cggcgatccc gtcatcgctc attcatccag gcatttattg
     7801 ctatgacatg ttctatcaga aaggaaaaac tccttttctg gcatggtgtg agcagcgagg
     7861 ctcaaagcgt aatgctgatg gtttaggaat gctggtggca caggcggctc atgcctttct
     7921 tctctggcac ggtgttctgc ctgacgtaga accagttata aagcaattgc aggaggaatt
     7981 gtccgcgtga atcaggccat ccagtttccg gacagggaag agtgggacga gaataaaaaa
     8041 tgtgtatgtt ttcccgctct cgtgaatggt atgcaactga catgcgcgat ctctggcgag
     8101 agtctggcgt atcgctttac tggagatacg ccagaacagt ggttagcgag ttttcgtcag
     8161 catcgctggg acctggaaga agaagcggaa aacttaattc aggaacaaag tgaagatgat
     8221 caaggctggg tctggttacc ctgatccaga tattcgtcct tccatttcac gtaattattc
     8281 gcggaatagc gtaacccagc cttctcttca tcacttaacg ggcggatctg tttgacgggg
     8341 ctaccgagat acagatatcc gctctccagc cgtttatttt gtgggaccag actacccgca
     8401 ccaatcatca catcatcttc tactattgcg ccatcaagta aaattgagcc catcccaacc
     8461 aaaactcgat tgccaatggt gcagccgtgg agcatcacct tgtgaccaac agtgacatct
     8521 tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg atttatgagt gacatgcaac
     8581 atactgccat cctggatatt ggtgcgtgct ccgatctgta cataatgtac atctccacga
     8641 atcacaacga gcggccagat ccccacatca tcagccagac gaacgtcacc aatcacgaca
     8701 ctgctatcgt cgatcattac gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt
     8761 aaaacatcag acatacttac ctcagcaata aatgatttac taatgacttt gggggcatta
     8821 ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca
     8881 aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa aaatacttgt gcaaaatatt
     8941 gggatcccta taatgcgcct ccgttgagac gacaacgtga aacacttcac aggatggtcg
     9001 gaacaacgaa gagaa
//
LOCUS       DAJTNW010000129         8992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNW010000129 DAJTNW010000000
VERSION     DAJTNW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            149..2333
                     /gene="espL4"
                     /locus_tag="OO179_003624"
                     /pseudo
     CDS             149..2333
                     /gene="espL4"
                     /locus_tag="OO179_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2302..4039)
                     /gene="aceK"
                     /locus_tag="OO179_003625"
                     /pseudo
     CDS             complement(2302..4039)
                     /gene="aceK"
                     /locus_tag="OO179_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4223..5526)
                     /gene="aceA"
                     /locus_tag="OO179_003626"
                     /pseudo
     CDS             complement(4223..5526)
                     /gene="aceA"
                     /locus_tag="OO179_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5556..7157)
                     /gene="aceB"
                     /locus_tag="OO179_003627"
                     /pseudo
     CDS             complement(5556..7157)
                     /gene="aceB"
                     /locus_tag="OO179_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7426..8355)
                     /gene="metA"
                     /locus_tag="OO179_003628"
     CDS             complement(7426..8355)
                     /gene="metA"
                     /locus_tag="OO179_003628"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5533541.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8512..8955
                     /locus_tag="OO179_003629"
     CDS             8512..8955
                     /locus_tag="OO179_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5533542.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2358 a         2168 c         2167 g         2299 t
ORIGIN      
        1 aatcgcaaga tctcattcca ccgaaacttt tacggctgca ttatgagatt gagcgattta
       61 tgatattttt ttagttgtaa tcatattaaa ttttttcact gttctgatag ttaaaattca
      121 agacatcaat aaacaatgag atatttaaat gattactcgt attcctcgta gttctttctc
      181 tgcaaatatt aataatacag cccagacaaa tgaacaccaa accctgagtg aattgtttta
      241 taaagaactc gaggataaat tttctggcaa ggagctggcg acgcctctat taaaaagctt
      301 ctcagagaac tgtagacaaa atgttcgtca tatctttagc aacaaggatt ttgtcattaa
      361 attttccacg tccgtcttac aagctgataa gaaagaaatt acgataatta ataaaaacga
      421 aaacacgaca ctcactcaaa ccattgctac aatatttgaa gaatacctaa tggaaatttt
      481 acctcaacgc tcagacactc ttgataaaca agaattaaac ctaaaatcag atagaaaaga
      541 aaaagaattc ccaagaatta aacttaatgg tcaatgttat ttcccggggc gccccaaaac
      601 cgtatagtat accgacacat tgctgcacaa tatattaatg atatttatca gaatgttgat
      661 tacaaacccc atcaagatga ttactcttca gctgaaaaat ttctcactca cttcaacaaa
      721 aaatgcaaaa accagacttt ggcgttgatt tccagccgtc ctgaggggcg ttgcgttgct
      781 gcctgcggtg atttcgggct agttatgaaa gcatattttg acaagatgga atcaaatggc
      841 atcagtgtta tggcagccat attactggtg gataaccatg ctttgacggt ccggctaaga
      901 ataaagaaca caactgaagg atgtacccat tacgtggttt cggtttatga tcctaatgta
      961 actaacgata aaataagaat tatgagcgaa agcaaagagg atattaaaca ctattctctg
     1021 atggatttta tgaatgtaga ttatagcctc ctgaaatggt caaatgatca tgttattaac
     1081 caatctgttg caataattcc agcacttccg aaagaacagc tattgatgtt aaaaggaact
     1141 gtggatgaaa taacccctcc attatcacca gcaacgatga atttgctaat ggcaattggt
     1201 cagaatcacc aactcacgca actgatgatt cagctccaga aaatgccaga actacataga
     1261 acagaaatgt tgactgccta taatagtatt aacctccccg gtttatattt ggctataaat
     1321 tatggcaatg cggatatcgt tgagactatt ttcaattcat tgtcagaaac aggatatgaa
     1381 gggttactct cgaaaaaaaa tctcatgcat attctggagg caaaagataa aaatggtttt
     1441 tctggattat ttttagcgat atcacgtaag gataaaaatg ttgtaacctc gattctgaac
     1501 gtcttaccta aactggccac aacacatcat ttagataatg aacaggtgta taaattcctg
     1561 agtgccaaaa atagaacgtc cagccatgtt ttataccatg ttatggcgaa tggtgatgcc
     1621 gacatgctga aaattttttt ggtcgcgtta cctttgttaa ttcgcacatg tcatttgact
     1681 aaagaacagg tactcgatct cctgaaggca aaggattttt atggttgccc aagactatac
     1741 ctggcgatgc aaaatggaca tagcgatatc gtgaaagtta ttctcgaagc attgccctgt
     1801 ctggcccagg aaattaacat ttcagcttcc gatattgtag atcttctgac cgctaaaagt
     1861 cttgcgcgcg acacgggttt gtttatggcc atgcagcgcg gacatatgaa cgttattaat
     1921 actattttta acgcattacc cactcttttt aatacgttta aattcgataa aaaaatatga
     1981 agcccctcct cctggcaaat aattctaatg aatatcccgg tttgttttca gcgatacagc
     2041 ataaacaaca aaacgttgta gagacggttt atcttgcttt atctgaccat gcacgcctgt
     2101 ttggatttac cgctgaagat attatggatt tttggcaaca caaagcgcca caaaaatact
     2161 ctgcctttga gttggctttt gaatttggtc accgggttat tgctgaatta atccttaata
     2221 cattaaataa gatggctgaa agctttggct ttacggataa ccctcgatac attgcggaga
     2281 aaaattatat ggaagcttta ctcaaaaaag catctcccca taccgtacgc taaatctttg
     2341 cctgcgccga tacgcataaa catcttccac atgcccttca cgtatgcggt tttgtagtgc
     2401 gcgccagtaa tcagcgcgga acaggtcggc gtgcatctct tcaaacagcg gaccaatacg
     2461 cgggtcggcg catagccagt ggcgaaactc ttccgggaaa acatcgcccg gcgagacgct
     2521 gtaccacggt tcgctggcaa gttcgtcttc cggatagcgc ggcggcggga tgtcgcggaa
     2581 attcacttcc gtcatgtagc aaatttcatc gtaatcataa aaaaccacac gcccgtgacg
     2641 ggtgacaccg aagtttttaa acagcatgtc gccagggaaa atgttagcag cggcaagctg
     2701 gcgaatagcg ttaccgtatt cttcaatggc gtcgcgcaac tgctgacctt ccacttgttc
     2761 cagccagata ttgagcggca ccatccgccg ctcgattata gagatggcga attacaatct
     2821 gctcaccaag atcggtgatt ttttccgctg cttcctggag cagtaattcc attaataccg
     2881 gggaaatatg ccgcttctcc agcacaaagt tttcaaactc ctgggtgtcc gccattcggc
     2941 ccacacggtc gtgctctttc accagttgat agcaggcacg aacgtgagcg gcagacatct
     3001 ctttctgcgg cgcgaacttg tctttgatga ctttgaatac ccgatcaaag cccggcagcg
     3061 taaacaccaa catcaccatt ccacgaatac ccggcgcttc aatgaactgc tcattacagc
     3121 cctgtagata aacgagatat tcgcggtagc tttcggtttt ggcgtgcttc tggcagccga
     3181 tagccatata caattcagca gtggttttac ctggcagaat ttcccgtagc cactcgacca
     3241 gcgctgcggg cagcggtgca taaaccataa aataagaacg cgcaaagcca aaaacaatgc
     3301 tcgcttcggc ggtcgtcgtc aggcaggtat caataaataa ctcgccgtcg tccgtctggt
     3361 ggatcggcag taagaagggc aacgtgccgg aaggcgtgat cagtttgccc accagccagg
     3421 cggctttatt gcggtaaaac agttcgttcg ccacctgtaa atgactttcc gcgaggttgt
     3481 ctgtccccag cgtttccgtc agatggcgaa tgatgtaatg aatgtcacgg cttttattct
     3541 gccagcgcag gcgcagcggc aggtcgctga taacgcgcat cagtagagat tcccagccgt
     3601 gatcggggtg aaagtctttc gccagcgggc ggggaatggt acgaaagcgg cgctctggct
     3661 gagagctaaa gataaaaagc cgctcgggag taagcgagcg gtggtcaaat aaccgacagt
     3721 acacggagtt aaaaaagctc tccgcaatct cgaagcgcgg gtaatccggc aacagccgcg
     3781 tgtaatgctc tttaacgcgc agtaaaaatg ccgcgtccgt actttggccg ttagtaatgc
     3841 agcgcagttg ctccacgacc agaccaacgt gatgatcgta aagatggata cggtttttca
     3901 tcgcctgctg gacagcatgc cagtcggcct gttcgaaacg ctgctgcgcc ccggaagtca
     3961 cttcgaggaa tcgaccatac tgagcgtcga agccttgcaa aatggtttga gcaatcagta
     4021 attccaggcc acgcggcatt tagcgccctc atcaggagca gagaattgcc ggatgcggcg
     4081 taaacgcctt atccagccta cgttcggcaa cggttgtagg catgataaga cgcgcaagcg
     4141 tcgcatcagg catcgtgcac cgattgccgg atgcggcgta aatgccttat ccggcctaca
     4201 gtcagcaacg tttgtaggtt gcttagaact gcgattcttc agtggagcca gtcagcgcgg
     4261 tgactgaaga cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca
     4321 cttcctgctg gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct
     4381 gcactttctc aacatagtgc ttcatacctt cgccctgcgc ataagcgttt gccaggtcga
     4441 acatgttaaa ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca
     4501 tatccgacag ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt
     4561 tgaacgacgg cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt
     4621 gtgcaaagcg acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag
     4681 catatggcgc atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga
     4741 agaagccttc actggtacgc tcgccggtaa taaattcgct gtcatacggg tcgcaatctg
     4801 aggtgatcag atccgccgca tcagcatcgg tacgtgcaac cagcagggtt ggaacgcccg
     4861 tcacgtcagc tgccagacgc gccgcgacca gtttctgaat agcttcctga gttggcacta
     4921 aaactttgcc gcccatgtga ccgcatttct tcactgacgc cagctgatct tcgaagtgaa
     4981 ctgccgcagc accggcttca atcatcgctt tcatcagttc aaaggcattc aggacaccgc
     5041 caaaaccggc ttccgcatcg gcaacgatcg gcaggaagta atcgacatag cgcggatcgc
     5101 ccggctcaat gcccacggac cattggatct gatcggcacg acggaaggtg ttgttgatcc
     5161 gctccaccac agctggcacc gagtttgccg gatagagcga ctgatccgga tacatgctgg
     5221 ccgccaggtt agcgtccgcc gctacctgcc atcccgacag atagactgct tcaatacccg
     5281 ctttcgcctg ttgcagcgcc tgaccgccag tcagtgcacc gaggctgttg atgtagcttt
     5341 tttcgactca ccgtgcagca gacgccacat tttcgctgcg cccagttgcg ccagcgtgca
     5401 ttcaggattg actgaaccgc gtaatttcac cacatcttcc gcactgtatg ggcgagtaat
     5461 gccttcccaa cgcggttgag tccactcttt ctgtaattct tcaatttgtt gtgtacgggt
     5521 tttcatgtgc agatgctcca tagttatgtg gtggtttacg ctaacaggcg gtagcctggc
     5581 agggtcagga aatcaattaa ctcatcggaa gtggtgatct gttccatcaa gcgtgcggca
     5641 tcgtcaaaac ggccctggga gaaacgttct tcgcccagtt cgctggcaat gactttcatc
     5701 tcttcgccca gcatctggcg gaacaaggct ttggtcaccg gtttgccatt gctcaacgtt
     5761 ttttgatgat ggatccactg ccagatcgag gtacgggaaa tttcagccgt cgccgcatct
     5821 tccatcaggc cataaatcgg cacgcagccg ttgccggaga tccacgcttc gatgtactgc
     5881 acagccacgc gaatgttagc gcgcatacct tcttcggtgc gttcaccatc acaaggtgcc
     5941 agcagctgat cggcagtaat cggcgcgtct tgttcgcgca atacttcaag ctgattttta
     6001 cgggagccga gaatgtcgtt gaataccgcc attgccgtgt ccgcaaggcc tgggtgagcg
     6061 atccatgtgc catcgtgacc gttattggct tccagcgctt tatccgcttt tactttgttg
     6121 agcacctggt tattacgctc ttcatctttg ctcggaataa acgcggccat gccgcccatc
     6181 gcaaaagcac cgcgtttatg gcaggtttta atcagcaggc gtgagtaagc attcaggaat
     6241 ggtttatcca tcgtcacggc ctgtctgtct ggcaggacac gatcgggata gtttttcaac
     6301 gttttgatat agctgaagat gtaatcccag cgaccgcagt tcagaccaac aatatggtcg
     6361 cgcagcgcgt gaaggatttc gtccatctgg aacacggcgg gcagcgtttc aatcagcaac
     6421 gtcgccttga tggtgccgcg cggcagatta aagcgatctt ctgcatagct gaagacttcg
     6481 ctccaccagg ccgcttcctg ctaggactgg gttttcggca gatagaaata gggaccgctg
     6541 ccctttgcca acagtgcctg atagttgtgg aagaaataga gcgcaaaatc aaacaggcta
     6601 ccggggattg cctcaccacg ccaggtgaca tgtttttccg gcaagtgcag accgcgtacc
     6661 cgacaaatca aaaccgctgg attgggcttg agctgataaa ttttgcctgc ttcattggta
     6721 tagctgatgg taccgttaac cgcatcgcgc aggttaattt gcccgtcgat cactttgttc
     6781 cagtctggtg ccagtgaatc ttcgaaatcg gccataaaga ctttcacatt ggcgttcagt
     6841 gcgttgatca ccatcttgcg ctctaccggg ccagttatct ctacgcggcg gtcttctaag
     6901 tccgcaggaa tcccgcgaat tttccaatca gcatcgcgaa tggaagccgt ttccgaaata
     6961 aaatcaggca acgttccgtt atcaatatct tgctgctgct gaatgcgcgc tgccagaagt
     7021 ttattgcgtt gtggcgtaaa atgcgtcacc agctcagtca gaaattctac cgcttcggca
     7081 gtaagaattt gcttctcctg ctcgccatac ggccttgtga aagccagttc atcggttgtt
     7141 gttgcctgtt cagtcatcgt gcagctcctc gtcatggatc cgaaaacttc ccctctgtga
     7201 acgaaggaac gttgtgcgct tttcgttcag cacaactaag actactcatt taaaatgcaa
     7261 aatcaaaaac aatttccatt ttaattaaaa atacttgata acttattgat aacaatatga
     7321 ataaaattta ttcacttgct gaggtgcgtt tcggaaataa aaaaggcacc cgaaggtgcc
     7381 tgaggtaagg tgctgaatcg cttaacgatc gactatcaca gaagattaat ccagcgttgg
     7441 attcatgtgc cgtagatcgt atggcgtgat ctgatagacg taatagttga gccagttggt
     7501 aaacagcaaa ttaccgtgac tacgccagct cgctcgcggt ttgttttgcg gatcgttgtg
     7561 cgggaaatag ttatacggta catccgggtc tagtccggct tccacatcgc gtaaatattc
     7621 ctgcgccagc gtttgcgcat catattcggg atggcccgtc acaaaggcaa tgcgcttatc
     7681 tttgctggca aacagatatg catccccttc ttccgtctct gccagaattt ccagatcggt
     7741 gtaatcacga atcaacgctg ccggaaagtc agcatagcgc gaatgcggtg ccaggaatga
     7801 atcatcaaag ccacgcgtca gaagcgcatg aggatggaga atatgatgct cgtaaacgcc
     7861 agagagtttg tcggtgcgag tttgcttagg aatgccgtag aggatattga gcgcggcctg
     7921 taccgcccag cagacaaaca gcgtcgaggt gacgtgatct ttcgaccact ccagcacctg
     7981 tttgatctgc ggccagtaag cgacatcatt aaactccacc aggcccagcg gcgcaccggt
     8041 tacaatcaga ccgtcaaagt tctgttcctg aatatcttca aagttacagt agaagttgtt
     8101 cagatgctct gcgggcgtgt tgcgcgattc acgggaatcg atgcgcaaca gctgaatatc
     8161 gacctgcaaa ggtgagtttg aaagcaggcg cagaaactga ttttcagttt caatcttctt
     8221 cggcatcagg ttaaggatca gaaccttaag tggacgaatt tcctgaccag acgcacgaga
     8281 agttgtcatc acaaagacgt tttcttcacg caagaaattg acggcgggta gctcgtccgg
     8341 cacacgaatc ggcataacct gattacctca ctacatacgc ttatacgttt agacatccag
     8401 atagctgaag ataaccagaa aatttgccaa tgtcgagcct gcatgttgaa ggtgagaaag
     8461 tttcaaggaa aaggctacgt tagaatataa gaatgacgaa aaggagagaa aatggttatt
     8521 agtattcgcc gctcacggca tgaggaaggg gaggaactcg ttgcgatttg gtgtcgatct
     8581 gtcgatgcca cccacgattt tttatcagca gagtatcggg ccgagctgga agagctggtg
     8641 cgttcctttc tgccggaagc accgttgtgg gtcgcggtta atgaacggga gcagccggtt
     8701 gggtttatgt tgttaagtgg gcagcatatg gatgcgctgt ttatcgatcc tgatgtgtgc
     8761 ggctgcggcg tgggtcggat gctggtggag catgcgctct cgatggcacc ggaactgaca
     8821 accaacgtta atgagcaaaa tgagcaggcg gttgggttct ataagaaggt gggttttaag
     8881 gttacgggac gctctgaggt ggacgatttg gggaaaccgt atccgttgct gaatctggcg
     8941 tatgtggggg aataagccgc ccctgccgtg cctgcccaag ctggagaaga cg
//
LOCUS       DAJTNW010000130         8897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNW010000130 DAJTNW010000000
VERSION     DAJTNW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..2887
                     /locus_tag="OO179_003630"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO179_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO179_003631"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO179_003631"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5533543.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO179_003632"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO179_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO179_003633"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO179_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OO179_003634"
     CDS             complement(7280..8041)
                     /locus_tag="OO179_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5533544.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OO179_003635"
     CDS             complement(8038..8697)
                     /locus_tag="OO179_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5533545.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtt
//
LOCUS       DAJTNW010000131         8636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNW010000131 DAJTNW010000000
VERSION     DAJTNW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO179_003636"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO179_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5533546.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="OO179_003637"
     CDS             complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="OO179_003637"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5533547.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="OO179_003638"
     CDS             complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="OO179_003638"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5533548.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="OO179_003639"
     CDS             complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="OO179_003639"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5533549.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5260..7806
                     /gene="cyaA"
                     /locus_tag="OO179_003640"
     CDS             5260..7806
                     /gene="cyaA"
                     /locus_tag="OO179_003640"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5533550.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="OO179_003641"
     CDS             complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="OO179_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5533551.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8214..>8636)
                     /locus_tag="OO179_003642"
     CDS             complement(8214..>8636)
                     /locus_tag="OO179_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5533552.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2233 c         2220 g         2217 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc
     2101 tgcatcagtt tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata
     2161 ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta
     2221 tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc
     2281 tgatgacgcg gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga
     2341 tagatatcct gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta
     2401 atgaagttgt ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg
     2461 gaactggaaa gctcttcacc gtctgaatcc atcggcgaac catcgctgtc attatcggcc
     2521 agacgcaggt tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa
     2581 tccacctgcg atactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta
     2641 atcagactcg ggtcattcat atccgccagg ctggcgtctg cacttttcag caacgctgca
     2701 gaggtcgtga cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg
     2761 gcctgagcca gcagccaggt tttagcatcg ctgccggaaa tggtggcgat cttttgttgg
     2821 acttcatcca actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgcgca
     2881 gcttgttgct taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat
     2941 gccgtcagtt ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta
     3001 ccccagccat acaaaccgac acccgccgcc agagcaatag cgatagccac cgcgctgaga
     3061 atcaatgcag tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc
     3121 acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct
     3181 tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg
     3241 accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc
     3301 aatagtcgac agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt
     3361 tgctgcaaca tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc
     3421 atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg
     3481 acctcagcac cgcgcaccgt cagggtatcc ccaattagct cacgaccgcc attgccacgt
     3541 aatatcagcg cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg
     3601 ctgatttccc gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt
     3661 gcggtggtgc gtccaatggc gaaataagta ggtagttggg gccatttacg atcttgctga
     3721 tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc
     3781 tcccccagcg ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca
     3841 atcagcggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct
     3901 ccagcgggag acgggcgggt gaccaggata ctcatgccgg ggcctctccg ttatagactt
     3961 cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt
     4021 gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg
     4081 cgccgaccag cgcacatagc cagatttcgc catcaataag ctcggcgtag ctaccaattg
     4141 gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg
     4201 cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc
     4261 attcaatacc gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg
     4321 cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat
     4381 cgtattcgcc gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga
     4441 taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga
     4501 cgatactgcc tgctggtaac gcatccagac tgtcatagtt attggacaca aaggcatcgc
     4561 gaggatcttc acgctcgcaa atagtgacca gccccagacc ttgcgggaat tcaaccggca
     4621 catctttcat tgaatgtacg gcgatatcag cgcgattctc caggagcgcg acttccagct
     4681 cttttacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc
     4741 cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc atcaacttgt
     4801 ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa
     4861 cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc
     4921 agagtgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct
     4981 ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac
     5041 ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg
     5101 atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta
     5161 acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg
     5221 aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact
     5281 ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg
     5341 gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg
     5401 ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat
     5461 gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggat
     5521 ccaccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta
     5581 gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc
     5641 gaagagcgcc agttactaca acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg
     5701 ggtgtggaag tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc
     5761 agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat
     5821 cgtactgccg tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa
     5881 gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat
     5941 gaatggctgg atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc
     6001 ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa aacactgctg
     6061 ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag
     6121 cgtttgcacg acggcgagat tgtatcgttt ggcctcgatc catactgcat gatgctggag
     6181 cgtgttactg aatacctgac ggcgattgaa gatttcaccc gtctggattt agtacgtcgc
     6241 tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt
     6301 cgcgcagtgt tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg
     6361 ctcgataacc gtgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagctgctc
     6421 gacgcgatga tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc
     6481 gtctccgcca gtccgcagga tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa
     6541 gcattgccag gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg
     6601 aatctgacct ttatttatgt gccgccgggc cgggctaacc gttcaggttg gtatctgtat
     6661 aaccgcgcgc caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac
     6721 ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg
     6781 tatattaaag gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg
     6841 tcgcaccatt tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt
     6901 gagattcgcc atctggcgat tatcgttaac ctggaatatg acccgacagc tgcgttccgc
     6961 aatcaggtgg tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa
     7021 aattgcctgg taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg
     7081 ctgcacttca acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg
     7141 catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc
     7201 ggcttaatcc gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc
     7261 agcacccgcc aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggc
     7321 ttgttcttcg aacgcctgaa tgtatcggta cagaagctgg agaacgccat cgagttttat
     7381 ggcgctattt cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc
     7441 aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt ctttttcgaa
     7501 gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag
     7561 gtgtatcacc actgcgaagg cagcaaagag gagctggtac gtgacgtcag tcgcttctac
     7621 tcgtcatcgc acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag
     7681 ttctatcaga ttgtgaaggt tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg
     7741 attggcaacc tgccgcccgc caatcaggat cacgatacgc cgctattaca gcagtatttt
     7801 tcgtgatgag cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac
     7861 tgtttcaccc gcctgttgcg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct
     7921 gcgatcgcaa atccattcat cgcctttcag gtcaaaatgg tagccgccct gtttggttgc
     7981 cagccacacc tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa
     8041 ggtaatggtc agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catccctgtc
     8101 gtccagacgc tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa attcactgtc
     8161 gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc
     8221 ttcaaagttt gcacctctgc tgctgcgttc cggcatgatt catccgtcac cggaataatg
     8281 atgtctctgt tttgaacttc acgtacgttc tcctctctga cacgagtttt cgccgggcag
     8341 ggggagggcg taatccttac ctctgtatcg gtccatgtat aaacattgcg atcgtcataa
     8401 gcgaagcgat cgtttttagt tagcgaaccg ttaactggaa taatttgcaa gctctttgac
     8461 cgtaattggc ggataaagta agcatgctgt ttgccgctat taaatgagcg atcaatgacc
     8521 aggtctggcg tatccagcgg gattatctca agctggtctt caccaaatgt cgataatttt
     8581 gttacataca gccgatcttt atattgcgca atattgccag taatcagatg gaattg
//
LOCUS       DAJTNW010000132         8415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNW010000132 DAJTNW010000000
VERSION     DAJTNW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO179_003643"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO179_003643"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5533553.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO179_003644"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO179_003644"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5533554.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO179_003645"
     CDS             3115..3399
                     /locus_tag="OO179_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5533555.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO179_003646"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO179_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5533556.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO179_003647"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO179_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5533557.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO179_003648"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO179_003648"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5533558.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2020 a         2253 c         2139 g         2003 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtggcatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt atttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gctta
//
LOCUS       DAJTNW010000133         8385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNW010000133 DAJTNW010000000
VERSION     DAJTNW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..805
                     /gene="ttdA"
                     /locus_tag="OO179_003649"
                     /pseudo
     CDS             1..805
                     /gene="ttdA"
                     /locus_tag="OO179_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            802..1407
                     /gene="ttdB"
                     /locus_tag="OO179_003650"
     CDS             802..1407
                     /gene="ttdB"
                     /locus_tag="OO179_003650"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5533559.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1455..2918
                     /gene="ttdT"
                     /locus_tag="OO179_003651"
     CDS             1455..2918
                     /gene="ttdT"
                     /locus_tag="OO179_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5533560.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="OO179_003652"
     CDS             complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="OO179_003652"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5533561.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4212..4427
                     /gene="rpsU"
                     /locus_tag="OO179_003653"
     CDS             4212..4427
                     /gene="rpsU"
                     /locus_tag="OO179_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5533562.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4538..6283
                     /gene="dnaG"
                     /locus_tag="OO179_003654"
     CDS             4538..6283
                     /gene="dnaG"
                     /locus_tag="OO179_003654"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5533563.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6478..8319
                     /gene="rpoD"
                     /locus_tag="OO179_003655"
     CDS             6478..8319
                     /gene="rpoD"
                     /locus_tag="OO179_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5533564.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2022 a         2252 c         2249 g         1862 t
ORIGIN      
        1 ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
       61 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
      121 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
      181 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
      241 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
      301 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
      361 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
      421 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
      481 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
      541 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
      601 attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
      661 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca
      721 gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct
      781 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
      841 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc
      901 gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc
      961 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gtgacaaatg ggagatggtc
     1021 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag
     1081 accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc
     1141 cagaaattca aggcgttaca tgtgattttc ccagcaggct gcgcggtgct ggcggcaacc
     1201 caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg
     1261 gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac
     1321 ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
     1381 tgcgagcacg tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc
     1441 gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgcg ccgctggcgg
     1501 tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct
     1561 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
     1621 tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc
     1681 ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt
     1741 ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct
     1801 atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc
     1861 gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac
     1921 cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc
     1981 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
     2041 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact
     2101 tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt
     2161 tcctcgggat gttgccgctc agcattttac tggttctgct ggttccctgg ctggcttacg
     2221 tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac
     2281 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
     2341 cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg
     2401 tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag
     2461 cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc ggactcaaca
     2521 ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt ggttattcgc
     2581 cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac tttttcgcca
     2641 gcgccacggc gtatacctgc gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc
     2701 cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca
     2761 ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa
     2821 cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg ttgctggtga
     2881 ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg
     2941 ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc
     3001 acgctaacac cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg
     3061 atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt
     3121 tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc
     3181 atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta
     3241 tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg
     3301 ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc
     3361 ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt
     3421 ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac
     3481 gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc
     3541 acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc
     3601 gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc
     3661 ggaacgttcc aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg
     3721 actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac
     3781 tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg ggaggccagt
     3841 tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct
     3901 aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata
     3961 cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc
     4021 gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta
     4081 taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa
     4141 ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag
     4201 gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc
     4261 gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt
     4321 tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg
     4381 cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg
     4441 ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg
     4501 cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt
     4561 attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa
     4621 gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc
     4681 gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg
     4741 caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga
     4801 gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat
     4861 cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca
     4921 acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt
     4981 aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt
     5041 cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt
     5101 ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga tgttccccat
     5161 tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc
     5221 caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct
     5281 ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat
     5341 ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc
     5401 aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta
     5461 tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta
     5521 catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac
     5581 gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact
     5641 ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg
     5701 cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg
     5761 aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga
     5821 acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg
     5881 cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt
     5941 cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact
     6001 ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg
     6061 tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa
     6121 gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga
     6181 actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg
     6241 cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc
     6301 cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt
     6361 acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa
     6421 ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg
     6481 gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat
     6541 ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc
     6601 gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat
     6661 gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg
     6721 caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac
     6781 atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag
     6841 cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc
     6901 acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg
     6961 atcactggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc
     7021 ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc
     7081 gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt
     7141 gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt
     7201 cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg
     7261 gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt
     7321 acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac
     7381 ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg
     7441 atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg
     7501 caaaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc
     7561 aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa
     7621 gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc
     7681 cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa attcgaatac
     7741 cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt
     7801 tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac
     7861 aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa
     7921 gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc
     7981 aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat
     8041 ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt
     8101 gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg
     8161 cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac
     8221 gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg
     8281 agccgttctg aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt
     8341 tacgccgcac ccggcactag tctctctgca caaacgccac ctttt
//
LOCUS       DAJTNW010000134         8275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNW010000134 DAJTNW010000000
VERSION     DAJTNW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            68..316
                     /locus_tag="OO179_003656"
     CDS             68..316
                     /locus_tag="OO179_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5533565.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="OO179_003657"
     CDS             381..749
                     /locus_tag="OO179_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5533566.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="OO179_003658"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="OO179_003658"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5533567.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="OO179_003659"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="OO179_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5533568.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OO179_003660"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OO179_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5533569.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OO179_003661"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OO179_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5533570.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OO179_003662"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OO179_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2018 a         2310 c         2018 g         1929 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc gcaggtagac tggcc
//
LOCUS       DAJTNW010000135         8248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNW010000135 DAJTNW010000000
VERSION     DAJTNW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1153
                     /locus_tag="OO179_003663"
     CDS             <1..1153
                     /locus_tag="OO179_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5533571.1"
                     /translation="MPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRAD
                     DLRDERSYRRRIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVS
                     DAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAG
                     FDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTT
                     GFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKE
                     EKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSF
                     SIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            1198..1422
                     /locus_tag="OO179_003664"
     CDS             1198..1422
                     /locus_tag="OO179_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533572.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1400..1900
                     /locus_tag="OO179_003665"
     CDS             1400..1900
                     /locus_tag="OO179_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5533573.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1900..3318
                     /locus_tag="OO179_003666"
     CDS             1900..3318
                     /locus_tag="OO179_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5533574.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3318..4115
                     /locus_tag="OO179_003667"
     CDS             3318..4115
                     /locus_tag="OO179_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533575.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            4115..4570
                     /locus_tag="OO179_003668"
     CDS             4115..4570
                     /locus_tag="OO179_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5533576.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4573..5181
                     /locus_tag="OO179_003669"
     CDS             4573..5181
                     /locus_tag="OO179_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5533577.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            5191..6522
                     /locus_tag="OO179_003670"
     CDS             5191..6522
                     /locus_tag="OO179_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5533578.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            6523..>8248
                     /locus_tag="OO179_003671"
     CDS             6523..>8248
                     /locus_tag="OO179_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5533579.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEV"
BASE COUNT         2173 a         2028 c         2162 g         1885 t
ORIGIN      
        1 gatgcctgtt gagcaggaag cgccaaccca gactggctgg gatttaactg gcaacgcaac
       61 cgggattctg gaactctccg tgaagtgcaa catgggcgat ccggataacg atttcttcga
      121 gcttcgtgca gatgacctgc gtgatgagcg ttcttaccgt cgccgcatcc aggcatccgc
      181 caaaaaactg gcgaataaca ttgagtcagc gattgccaaa caggcaactg aaatgggctc
      241 gcttgttgtt cacgataccc gcgcaattgg tccatcgact ggcctgcctg gctgggattt
      301 tgtgtctgat gcagagcgac tgatgttctc ccgtgagcta aaccgcgaca tgggcatcag
      361 ttacttcctg aaccctgacg attaccgcaa agcaggccgc aacctggtag atggtgacat
      421 cttcgggcgc gttcctgaag aagcatatcg aaacggtact attcagcgtc agattgctgg
      481 ctttgatgaa attcttcgct caccgaaact tccggcagtt accaagtcaa ccgcgactgg
      541 tgtaactgtt tctggtgcgc agaagtttaa gccgcaggca tacacccttg ataccgatgg
      601 taacaaagag aacgtcgaca accgtgttgc aacggtgacc gtatcctcca ccaccggatt
      661 taagcgcggc gacaaaatca gcttcattgg tgtgaagttc ctgtctcaga tggcgaagaa
      721 tgtgctgact gatgacgcga ctttctcaat cacccgtgtg atcgatggta ctcacatcga
      781 aatcacgccg aagccgattg cgctggatga cgcgtcactg acaaaagaag agaaggctta
      841 cgctaacgta aacacctctc ttgctgatac cactccggta aacgttctga acgtggcaac
      901 aaccaccgct aacgtgttct gggctgatga ctcaatccgc ctgctgtctc agccgatccc
      961 ggtaacccat gaactgtttg ctggcatgaa aacgtcttcc ttcagcattc caggtattgg
     1021 tgttaacggc atcttcgcaa cgcagggtga tatcaacact ctgtctggta agtgccgtat
     1081 tgctgtgtgg tattcagcat gtgctgtacg accagaggca attggtgttg gtctgcctaa
     1141 ccagaccgcg tgataaccag agggagcttc ggctcccgtt tttatctgga gacaagcatg
     1201 acacacatga tctttcgtca tggcgacatg aagaagtgga aaggcgttgg ctacgacttt
     1261 gaaatcgtga aagccgaaga gcttcaggaa tatctggatg ctggctggtt ttcacatcct
     1321 gatgaccttt tgaaggatgt tgcagagcca gaagaaaaac agtgtaaaaa gcctggtcga
     1381 aaacctaagg cggcagcaga tgaacctgac aacgaagggt gatttagttc ttgcggcatt
     1441 acgtaagctc ggtgttgcat caaatgccac gttaaccgat gtcgaaccgc agtctatgga
     1501 agacggcgtc aacgaccttg aaatgatgat ggctgaatgg cttggcggtg atgcgtcacc
     1561 tggtatcaac gttggctaca tttttgctga tgcagatgtt gctccggatc cgggcgatga
     1621 gcacggttta tcaaataacg ctatcaatgc cgtcattttc aaccttgcct gccgaattgc
     1681 tccagattat gcgctggaag cgtctgcaaa acttataacc actgccagat acgggaaaga
     1741 gcgactcgtc aaactgtctg caatggacag ggcaaaagca gctaaatgta agtccggtta
     1801 tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg aagtggaatg gttggaatta
     1861 cttccaccga aaggaacctt gcgataacgg gagcgaataa tgcctattca gcaacttccg
     1921 cttatgaaag gtgtcggcaa agacttccga aacgccgact atatcgacta tctgccagtg
     1981 aatatgctgg ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg
     2041 ggcattgcca aacgttctga cgtgaacggt gtatctcgcg gcgtcgagta caacatggcg
     2101 cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca aaggagaaag tgaagtcggt
     2161 gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc ggacatcaca ggcggtaggc
     2221 gttaatggtc aactggtcga gtatcgctat gatggcacgg tcaaaaccgt ctcaaactgg
     2281 cctacagaca gtggattcac tcagtatgag ttaggttcag ttcgcgacat tacgcgctta
     2341 cgtgggcgtt atgcgtggtc aaaagacgga actgattcat ggtttatcac tgaccttgaa
     2401 gacgaatctc atcctgaccg ttacagcgca caatatcgtg ccgagtctca gcctgacggc
     2461 atcatcggta tcgggacatg gcgagacttc atcgtctgct ttggttcatc gactattgaa
     2521 tatttctccc tgactggcgc aaccaccgtt ggtgccgctt tgtatgttgc acaaccatcg
     2581 ctgatggtgc agaaaggcat tgccggaact tactgcaaaa cgccattcgc tgattcgtat
     2641 gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt atatcatcga ctccggtcag
     2701 gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc gctcctacac tgctgatgaa
     2761 ctggctgatg gtgtgatgga atcgctgcga tttgatgctc atgagttgct gattatccac
     2821 cttccgcgtc acgtcctcgt gtacgacgca tcttcaagcg ccaatggccc acaatggtgt
     2881 gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta tcgacttcgt ttacgaaggc
     2941 aatcagataa cgtgcggcga taagctggaa tctgttaccg ggaaactgca attcgatatc
     3001 agcagccagt acgacaagca acaggaacac ctgctgttta ctccactctt caaagcggat
     3061 aacgccagag tgttcgacct tgaggttgaa tcgtcaactg gcgttgcgca gtacgccgac
     3121 cgcctttttc tctctgcaac cactgactgc atcaattacg ggcgtgagca gatgattgag
     3181 cagaatgaac cgttcgttta cgacaagcgt gttttgtgga agcgagtagg gcgcatcagg
     3241 aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac ctgtcactct gtctggcgct
     3301 cagataagga ttgagtaatg gctgattcga atctcaatgt gccggtaatc atccaagcta
     3361 cgcggctcga tacatcagtc cttccacgca atatcttctc gcagtcgtat ctgctttacg
     3421 ttatcgcaca gggcactgat gttggtaacg tggctaacaa agccaacgag gccggacagg
     3481 gcgcttatga tgcacaggtc aggaacgatg agcaggatgt gattctcgct gaccatgagc
     3541 agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga ggagcgaatc agacaggcag
     3601 aatcaactct tcaggaccat gaaacacgaa tagctcagaa tgaaagcgat atttcgtcgc
     3661 ttgacacaag agttcagtcg ctggagtcgc aggtttcaga ccatgaaacg cgcatcgatg
     3721 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
     3781 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
     3841 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
     3901 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
     3961 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
     4021 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
     4081 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
     4141 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
     4201 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
     4261 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
     4321 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
     4381 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
     4441 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
     4501 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
     4561 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
     4621 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
     4681 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     4741 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     4801 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     4861 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     4921 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     4981 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     5041 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     5101 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     5161 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     5221 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     5281 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     5341 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     5401 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     5461 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     5521 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     5581 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     5641 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     5701 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     5761 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     5821 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     5881 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     5941 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     6001 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     6061 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     6121 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     6181 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     6241 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     6301 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     6361 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     6421 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     6481 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     6541 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     6601 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     6661 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     6721 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     6781 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     6841 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     6901 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     6961 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     7021 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     7081 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     7141 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     7201 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     7261 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     7321 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     7381 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     7441 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     7501 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     7561 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     7621 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     7681 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     7741 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     7801 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     7861 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     7921 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     7981 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     8041 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     8101 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     8161 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     8221 gaccctattg aaaagagagt tgaggtag
//
LOCUS       DAJTNW010000136         7918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNW010000136 DAJTNW010000000
VERSION     DAJTNW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            237..740
                     /gene="dps"
                     /locus_tag="OO179_003672"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="OO179_003672"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5533580.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="OO179_003673"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="OO179_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5533581.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="OO179_003674"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="OO179_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5533582.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="OO179_003675"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="OO179_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5533583.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="OO179_003676"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="OO179_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OO179_003677"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OO179_003677"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5533584.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="OO179_003678"
     CDS             7091..7351
                     /locus_tag="OO179_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5533585.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7918
                     /locus_tag="OO179_003679"
     CDS             7616..>7918
                     /locus_tag="OO179_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5533586.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         1934 a         2025 c         1979 g         1980 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcgtg
//
LOCUS       DAJTNW010000137         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNW010000137 DAJTNW010000000
VERSION     DAJTNW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(123..953)
                     /locus_tag="OO179_003680"
     CDS             complement(123..953)
                     /locus_tag="OO179_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5533587.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO179_003681"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO179_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5533588.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO179_003682"
     CDS             2379..2729
                     /locus_tag="OO179_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5533589.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO179_003683"
     CDS             2855..3415
                     /locus_tag="OO179_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5533590.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO179_003684"
     CDS             3418..6384
                     /locus_tag="OO179_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5533591.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO179_003685"
     CDS             6451..6828
                     /locus_tag="OO179_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5533592.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO179_003686"
     CDS             complement(7029..7688)
                     /locus_tag="OO179_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5533593.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1783 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatatgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTNW010000138         7872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNW010000138 DAJTNW010000000
VERSION     DAJTNW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OO179_003687"
     CDS             complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OO179_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5533594.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSL"
     gene            complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OO179_003688"
     CDS             complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OO179_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5533595.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1735..2148
                     /gene="hiuH"
                     /locus_tag="OO179_003689"
     CDS             1735..2148
                     /gene="hiuH"
                     /locus_tag="OO179_003689"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5533596.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2257..3261
                     /gene="msrP"
                     /locus_tag="OO179_003690"
                     /pseudo
     CDS             2257..3261
                     /gene="msrP"
                     /locus_tag="OO179_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3262..3897
                     /gene="msrQ"
                     /locus_tag="OO179_003691"
     CDS             3262..3897
                     /gene="msrQ"
                     /locus_tag="OO179_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5533597.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4155..4805
                     /gene="zinT"
                     /locus_tag="OO179_003692"
     CDS             4155..4805
                     /gene="zinT"
                     /locus_tag="OO179_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5533598.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5883..6152)
                     /locus_tag="OO179_003693"
     CDS             complement(5883..6152)
                     /locus_tag="OO179_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5533599.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6209..6877
                     /locus_tag="OO179_003694"
     CDS             6209..6877
                     /locus_tag="OO179_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5533600.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7193..>7872
                     /locus_tag="OO179_003695"
     CDS             7193..>7872
                     /locus_tag="OO179_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5533601.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT         2274 a         1763 c         1583 g         2252 t
ORIGIN      
        1 aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga aacagtatat
       61 tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt gttttttgat
      121 attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt aatgcattaa
      181 ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc tcaaaatctt
      241 tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta
      301 atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc gttacgccac
      361 tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat actgagcaca
      421 gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca agcatattat
      481 gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatgt
      541 taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg cctgctgcgc
      601 tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt gtccggttaa
      661 caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca tccatcatcc
      721 ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac tgcttgatct
      781 gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat
      841 tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata aaaagcaagg
      901 ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc
      961 catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag
     1021 cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc cccaaatttc
     1081 actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta gccaaagtaa
     1141 ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac tttgactgac
     1201 agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat gttgccttaa
     1261 ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca gataatcatt
     1321 tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag taaggcaaat
     1381 aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg
     1441 aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat aaagcccatc
     1501 tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca
     1561 ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa tatgccataa
     1621 atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct gtaaggttac
     1681 cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat aaacatgtta
     1741 aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc tttggtttat
     1801 gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg aaaacccgct
     1861 gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca acttaatacc
     1921 gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcgactact
     1981 ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt
     2041 ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg
     2101 cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg cgtaaatatg
     2161 taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa cagtaaacag
     2221 ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca atttttaaaa
     2281 gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt gttaaaagca
     2341 ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga tctgcttagc
     2401 tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga gttcagcaag
     2461 cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc cggttataac
     2521 aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg tagcctgaaa
     2581 accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcat
     2641 gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa
     2701 gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact ggcgcttgcc
     2761 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc ggaacagatg
     2821 cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg
     2881 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta tggcaaggcg
     2941 ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa
     3001 gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac cacctggaat
     3061 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
     3121 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga tgttcagcgc
     3181 cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg
     3241 gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg gctgaaagtt
     3301 tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt
     3361 ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa
     3421 tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     3481 atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc
     3541 agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa ggagttaatt
     3601 acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttc
     3661 acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt gcataacttc
     3721 gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa gattatttca
     3781 ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg gtataagaag
     3841 ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc tctataactt
     3901 catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc cctatccggc
     3961 ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca ccagaataat
     4021 ttattttatt tcaataagat agttcaatac acctgagaat accatgctct catttcctaa
     4081 tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt acattaactc
     4141 tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta ggtgtcttta
     4201 ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc ttaacagagg
     4261 tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac cgaacgctca
     4321 gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc aaacttgatc
     4381 ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa attaaagatt
     4441 attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac ggcattgtcg
     4501 aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga tacaaaatac
     4561 tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa
     4621 gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga aaatcatctc
     4681 attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa atggaaaact
     4741 ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag atgatgtctc
     4801 attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta gtaacaacaa
     4861 taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg agtcgtgtct
     4921 tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata cacaactcag
     4981 ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg gtaaagctaa
     5041 ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta tgatacaaag
     5101 gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt gtcccgcggc
     5161 tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact
     5221 tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg
     5281 tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagct
     5341 ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc
     5401 cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata
     5461 agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact
     5521 ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa tttttcttgg
     5581 aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt aaagaagaat
     5641 attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg tgatttgccc
     5701 cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt gttgaataat
     5761 tccactgcag ttatataaaa tcggtaacgg ctggaaatca ttcaatactc gcactatcga
     5821 aagtttacca gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt tttctttttc
     5881 gcttacattg tgtctggttc ttccggccac tcaatatcag gtgcagttga tgtatcaaca
     5941 cggttcagca acacccgata cttcttccag gcttccagca acgagttttc ttcctccgtt
     6001 gcgatctcca gatctacagc atcctgaagt ggcgcaatat gctcactgaa tccctggatg
     6061 tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga agcataccag
     6121 gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca tcgccataat
     6181 tgatttaatt cacaaataaa actataatat ggtgaaatta atgaaaaaaa acacagatga
     6241 tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg ttttgggagt
     6301 atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga
     6361 acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc ttactcacgt
     6421 acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc
     6481 tacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgaacctta
     6541 tcctgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc ttcactgcct
     6601 gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg ccttaactga
     6661 tgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca atagcttcgg
     6721 tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa aagattccga
     6781 agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca aggttcaagg
     6841 tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag cacgttcttg
     6901 cttaagacgt gctacggttt cctttacgat ttggggttgc gactttacct ctattgataa
     6961 tgcattccgg cagaacgttc aaatatgaac gtacgatatt taactaaccg aaaaacaaaa
     7021 tatagcacag gcgagacatt taccatctaa gaaaatagta tcgttttttt acagttaaat
     7081 caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt ttatttcagc
     7141 ttattattcc ccagtatcac attttatcag gatatccttc tgcgaggtta ttatgcttcc
     7201 tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta catcaatcga
     7261 caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca gcatttccgg
     7321 agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggagaaca gtgaaacatc
     7381 gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca atcttgatga
     7441 aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg cattgaaaga
     7501 gctgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt cgctggaata
     7561 tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt cactagaata
     7621 tattaatgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt cattggaatt
     7681 acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa gagatgatca
     7741 tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg aaagcatact
     7801 tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc ccagaataat
     7861 gcaaactttg tt
//
LOCUS       DAJTNW010000139         7774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNW010000139 DAJTNW010000000
VERSION     DAJTNW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            55..588
                     /gene="ymdB"
                     /locus_tag="OO179_003696"
     CDS             55..588
                     /gene="ymdB"
                     /locus_tag="OO179_003696"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5533602.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            530..2011
                     /gene="clsC"
                     /locus_tag="OO179_003697"
     CDS             530..2011
                     /gene="clsC"
                     /locus_tag="OO179_003697"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5533603.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="OO179_003698"
     CDS             complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="OO179_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5533604.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3570..5105
                     /gene="mdoG"
                     /locus_tag="OO179_003699"
     CDS             3570..5105
                     /gene="mdoG"
                     /locus_tag="OO179_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5533605.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5098..7641
                     /gene="mdoH"
                     /locus_tag="OO179_003700"
     CDS             5098..7641
                     /gene="mdoH"
                     /locus_tag="OO179_003700"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5533606.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1926 a         1861 c         2055 g         1932 t
ORIGIN      
        1 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
       61 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      121 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      181 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      241 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      301 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
      361 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
      421 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
      481 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
      541 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
      601 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
      661 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
      721 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
      781 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
      841 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
      901 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
      961 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1021 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1081 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1141 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1201 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1261 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1321 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     1381 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     1441 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     1501 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     1561 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     1621 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     1681 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     1741 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     1801 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     1861 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     1921 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     1981 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2041 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2101 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2161 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2221 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2281 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     2341 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     2401 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     2461 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     2521 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     2581 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     2641 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     2701 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     2761 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     2821 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     2881 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     2941 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3001 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3061 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3121 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3181 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3241 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3301 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     3361 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     3421 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     3481 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     3541 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     3601 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     3661 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     3721 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     3781 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     3841 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     3901 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     3961 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4021 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4081 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4141 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4201 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4261 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4321 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     4381 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     4441 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     4501 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     4561 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     4621 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     4681 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     4741 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     4801 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     4861 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     4921 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     4981 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5041 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5101 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5161 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5221 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5281 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     5341 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     5401 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     5461 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     5521 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     5581 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     5641 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     5701 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     5761 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     5821 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     5881 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     5941 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6001 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6061 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6121 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6181 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6241 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6301 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     6361 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     6421 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     6481 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     6541 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     6601 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     6661 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     6721 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     6781 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     6841 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     6901 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     6961 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7021 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7081 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7141 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7201 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7261 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7321 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     7381 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     7441 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     7501 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     7561 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     7621 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     7681 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     7741 acatccggca aaaacaacga gccaataaaa atac
//
LOCUS       DAJTNW010000140         7734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNW010000140 DAJTNW010000000
VERSION     DAJTNW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO179_003701"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO179_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5533607.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO179_003702"
     CDS             1102..2562
                     /locus_tag="OO179_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5533608.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO179_003703"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO179_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5533609.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO179_003704"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO179_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5533610.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO179_003705"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO179_003705"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5533611.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO179_003706"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO179_003706"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5533612.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO179_003707"
     CDS             complement(6727..7245)
                     /locus_tag="OO179_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5533613.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7734
                     /gene="ydeI"
                     /locus_tag="OO179_003708"
     CDS             7521..>7734
                     /gene="ydeI"
                     /locus_tag="OO179_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5533614.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLI"
BASE COUNT         1937 a         1809 c         1953 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg attt
//
LOCUS       DAJTNW010000141         7719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNW010000141 DAJTNW010000000
VERSION     DAJTNW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..830)
                     /locus_tag="OO179_003709"
     CDS             complement(<1..830)
                     /locus_tag="OO179_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5533615.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRLTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="OO179_003710"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="OO179_003710"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5533616.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="OO179_003711"
     CDS             complement(2479..2706)
                     /locus_tag="OO179_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5533617.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO179_003712"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO179_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5533618.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO179_003713"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO179_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5533619.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO179_003714"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO179_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5533620.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="OO179_003715"
     CDS             complement(5528..6511)
                     /locus_tag="OO179_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5533621.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="OO179_003716"
     CDS             complement(6557..7381)
                     /locus_tag="OO179_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5533622.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO179_003717"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO179_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5533623.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2012 c         1810 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttaatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcact
//
LOCUS       DAJTNW010000142         7555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTNW010000142 DAJTNW010000000
VERSION     DAJTNW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OO179_003718"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OO179_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OO179_003719"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OO179_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5533624.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OO179_003720"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OO179_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OO179_003721"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OO179_003721"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5533625.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OO179_003722"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OO179_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5533626.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO179_003723"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO179_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5533627.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OO179_003724"
     CDS             complement(6511..7203)
                     /locus_tag="OO179_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5533628.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1923 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgactatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tctggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg taggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggta ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagagaa agcaaaaata aatgattgac tctgtagcgg gaaagcgtat
     7501 tatgcacacc cccgcgccgc tgagaaaaag cgaagcgcac tgctctttaa caatt
//
LOCUS       DAJTNW010000143         7198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNW010000143 DAJTNW010000000
VERSION     DAJTNW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(17..415)
                     /gene="vapC"
                     /locus_tag="OO179_003725"
     CDS             complement(17..415)
                     /gene="vapC"
                     /locus_tag="OO179_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5533629.1"
                     /translation="MLKFILDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(415..642)
                     /gene="vapB"
                     /locus_tag="OO179_003726"
     CDS             complement(415..642)
                     /gene="vapB"
                     /locus_tag="OO179_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5533630.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            724..1083
                     /locus_tag="OO179_003727"
     CDS             724..1083
                     /locus_tag="OO179_003727"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5533631.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQKRC"
     gene            1077..5938
                     /gene="traI"
                     /locus_tag="OO179_003728"
                     /pseudo
     CDS             1077..5938
                     /gene="traI"
                     /locus_tag="OO179_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            5958..6704
                     /gene="traX"
                     /locus_tag="OO179_003729"
     CDS             5958..6704
                     /gene="traX"
                     /locus_tag="OO179_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5533632.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6759..>7198
                     /locus_tag="OO179_003730"
     CDS             6759..>7198
                     /locus_tag="OO179_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5533633.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDT"
BASE COUNT         1598 a         1861 c         2328 g         1411 t
ORIGIN      
        1 atttctgatg aacaggtcag ctccagtctt cagttctcag gccgcccaca cgttcaaatt
       61 cccgggtgtt attagtcaca ataatcagcc cccgactgcg ggcatgacct gcgatcattt
      121 gatcaaatgg cccgacaggg cgtccctgac gggcaagttc tgctcttatc tggccggtgt
      181 gtgtggctgc agcagcgtcg taatccagaa catcaatgcg ggaaacaaat ccctcgatca
      241 cagcgagatt acgttcaggc atctggcttt tttctgcacc atatatcagc tccatcaggg
      301 tgaccgaact gatacacatt ttcccctggt tcaggttaaa acgctccctg acgctggcgg
      361 gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag aataaacttc agcatcagaa
      421 tgactccctt tcctgcatgc cgggctgttc cctgttatcc ataaaatcgg tgctgacgct
      481 gtgtccgtcg aaccattcgt cccatgtctc tccggctggc gtaatgattc tggttcgtcc
      541 gacagcaatc acttcaacgc gttttacgtt ttccggcaat gcaaccgctt ttggcagtct
      601 gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc attatttgcc tccttatgtg
      661 gatatgtctt gtgtatatct attgtgggat tgataccggg atatgtcaaa gggatatacg
      721 tttatgatga gtatcgcgca ggtcagatcg gccggaagtg ccggtaatta ttataccgac
      781 aaggataatt actatgtgct gggcagcatg ggagaacgct gggccggcca gggggctgaa
      841 caactggggc tgcagggcag tgtcgataag gatgtcttta cccgccttct ggagggcagg
      901 ctgccggacg gggcggattt aagccgcatg caggatggca gtaacaggca tcgtcccggc
      961 tacgacctga ccttctccgc tcccaaaagt atctccatga tggccatgct cgggggggac
     1021 aaacgtctta ttgaagcaca taaccaggcc gtggattttg ctgtccgtca gaaacggtgc
     1081 tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat caggaaccac
     1141 agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa tggaagacac
     1201 tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct aatcagattg
     1261 cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg ggctatgaaa
     1321 ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg gaggcctttt
     1381 ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg aaatcccggg
     1441 atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa gtcagaatgg
     1501 ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat cgtgacgcag
     1561 cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag gacgggccgg
     1621 atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa gtgcagttta
     1681 tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat ggtgtgattg
     1741 aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc cccctcgatc
     1801 gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca gtccgggcac
     1861 tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa agtgtccccc
     1921 ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg tcgctggcca
     1981 ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa ctggtcatga
     2041 tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc tcgcagatga
     2101 acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt cagttgctgg
     2161 aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa aaactctccc
     2221 tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag gtcctgataa
     2281 ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag gatgccgggg
     2341 tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc agtgaaccgg
     2401 accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag aagagagcgt
     2461 ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc gcagtgagct
     2521 gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt caccggtctg
     2581 gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg tgatggagca
     2641 gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga cggcgcagag
     2701 tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta tttcttccct
     2761 ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg gtgagcgtct
     2821 gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc tgcaggtggc
     2881 atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac cggccaccct
     2941 gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg tggaaacgcc
     3001 cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga tggcaatgga
     3061 caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt attcctcact
     3121 ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg tggtttctga
     3181 gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc tgcagaaagc
     3241 cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc tggaaagtaa
     3301 aaaactggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg ctgcagaagg
     3361 aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg gtgatttact
     3421 gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg cgtcgtatga
     3481 ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca cgccgctgat
     3541 ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg ccgccacccg
     3601 tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg gtgtgggtaa
     3661 gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga gtgaacgtcc
     3721 ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca gcgccggcgt
     3781 ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc gcagcggaga
     3841 aacgccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg tgggcaatac
     3901 cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg tggccagcgg
     3961 tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc agcagacgcg
     4021 cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg aactgcggga
     4081 ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc ttgagcgtgt
     4141 gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact ccgtgacgga
     4201 gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga tgctgaaagg
     4261 cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg actataccgg
     4321 caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg aggaccggcg
     4381 cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg ggaaggtgca
     4441 ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc gtcgtctctc
     4501 cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc accggattgc
     4561 cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta acacgcggct
     4621 gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg acaccatccg
     4681 ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg gttatgtggc
     4741 caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt ctgacggtca
     4801 gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg acctggctta
     4861 tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg cgcttgaagg
     4921 cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg ccctgtcgcg
     4981 tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg atgccatcaa
     5041 caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg accgggaggt
     5101 catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg cggcaggccg
     5161 tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt ttattgctcc
     5221 gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta acggcaagtc
     5281 cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc ggggattcag
     5341 cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc agggcagccg
     5401 taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg cccgtgataa
     5461 tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga atcccggtgc
     5521 catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc cgggagccgg
     5581 aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag aggccatccg
     5641 ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga acaaacctga
     5701 cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg agcgtgaccg
     5761 ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg aaccacaacg
     5821 ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc gtctgcagca
     5881 gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg gagactgata
     5941 caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac gattcactgg
     6001 ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga cagcgggata
     6061 tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg atattccagc
     6121 tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt gccctggtgt
     6181 gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac cggctgtggg
     6241 gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg tatgagggga
     6301 atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg
     6361 ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg tccggcacca
     6421 gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac cgggcggaag
     6481 acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg gcgcttaacc
     6541 ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg accgttggtc
     6601 tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt ttcccgacgt
     6661 tctatgcatg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg tggtatcttt
     6721 ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag aagcgaccgg
     6781 tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg aaaaccatca
     6841 tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc gagaaagcgg
     6901 cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg tccatttatc
     6961 tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg ccgggattat
     7021 ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta ctggaagacg
     7081 tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg aaggccatca
     7141 cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat gacacgga
//
LOCUS       DAJTNW010000144         7098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTNW010000144 DAJTNW010000000
VERSION     DAJTNW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(296..1198)
                     /locus_tag="OO179_003731"
     CDS             complement(296..1198)
                     /locus_tag="OO179_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5533634.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO179_003732"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO179_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5533635.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO179_003733"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO179_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5533636.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO179_003734"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO179_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5533637.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO179_003735"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO179_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5533638.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO179_003736"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO179_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1646 a         1793 c         1861 g         1798 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagat
//
LOCUS       DAJTNW010000145         6951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNW010000145 DAJTNW010000000
VERSION     DAJTNW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO179_003737"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO179_003737"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5533639.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="OO179_003738"
     CDS             complement(1117..1575)
                     /locus_tag="OO179_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5533640.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="OO179_003739"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="OO179_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5533641.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="OO179_003740"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="OO179_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5533642.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="OO179_003741"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="OO179_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5533643.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO179_003742"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO179_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5533644.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="OO179_003743"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="OO179_003743"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5533645.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1833 a         1524 c         1700 g         1894 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gtgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctcct ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagctcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa agttgctttg ggtaaacaaa
     6841 aaaacggccc cagaaggggt aatgccagtc agttaagcaa ctgactggct ctttttcgga
     6901 gctgtggggt atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DAJTNW010000146         6858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNW010000146 DAJTNW010000000
VERSION     DAJTNW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO179_003744"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO179_003744"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5533646.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO179_003745"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO179_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5533647.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO179_003746"
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO179_003746"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5533648.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4131..4886
                     /locus_tag="OO179_003747"
     CDS             4131..4886
                     /locus_tag="OO179_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5533649.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="OO179_003748"
     tRNA            4965..5038
                     /locus_tag="OO179_003748"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5153..6757
                     /locus_tag="OO179_003749"
     CDS             5153..6757
                     /locus_tag="OO179_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5533650.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT         1707 a         1687 c         1789 g         1675 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagtcaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcctc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgt ccgctccatg
     5041 acctttctta aagtactcca acctactgaa tcctaaggga tatggcttta agcctccttg
     5101 cagagttact tactgtaacc cactgtgtaa ccctctcagg gcatacagac atatgattca
     5161 gctatccagt agtcacaaac ttccagcagt ctactatctc taccagcgta acggcgtcta
     5221 ctacttccgt cttcgggtca gacaatcaaa caatgataga atgacctcag tatcattacg
     5281 gaccaaggat agacgcaccg ctatggctca ctcaaggcac atcaaggcag cactcaaggc
     5341 aatacacgca gacagaccca acgctaccta tgaggagatg agggagcacc ttaaggacat
     5401 cgctgagtgt gaactgtcta tgggccgttc agatctcttt gagcctgaca tgagggatat
     5461 ctaccgggac caatacggag aacttggtga gagccttacc gatgctctgg ctagtgagcc
     5521 gctaagtatt gaccagcatc gttacatcaa tgaggccctg aaggtgctaa aagcctgtat
     5581 gaggcgtatc gaagcgggag atagtcagcc tctgatagat tacgtcgatc gttttaatga
     5641 tattgatcgg caagatgacc aagcagactc tgtgtcccta tctgttagtg ctccagaagt
     5701 aaaaccagaa gttacgccta gcattaccat agcgagcctc tttgagcaat acgaagcgga
     5761 gaactctcag aactggaagc ctgcaacctt aagggaaaac aaagcgtcac acgctgcgtt
     5821 aatcgaaata ttcgaccatc tgggtctcaa tgcagacgct aacagggctg acatgcttaa
     5881 ggtccgtgat gtgctccagc agttacctaa gaaccgtaag cagcgcttta aggatgcccc
     5941 acttgctgac ctgttgagcc gtgaggataa gacagattgt ctggatgtcg ttaccatcaa
     6001 caacaagtat cttattaaga tggctgctgt ctttaggtgg gctgtgcgca atgacttgat
     6061 taaaaagaac atgactgagg gtctggagct taaagtccct cagcgtaaag cctcaggggc
     6121 ccgtaacgcc ttctctacgg agcaggtggg gcaactactg gtcgcagcta aggcatactc
     6181 tcagaagacc tctggtaagc cctaccacta ttacgttacg gctttggctg ctatcactgg
     6241 ggcgagactc aatgaaatag cccaactaca ggtcaaagac gtcaggacca ctgaggcggg
     6301 aaccgtttat attcatatca atgaagacga tagtagtcta cccggtaaga gcatcaagaa
     6361 cgctcatagt gaccgctgtg tgcctctggt cgatggggcc tatggtttta tattggctga
     6421 ctttatggcg cttgtagaga cccgtagagg cgctgacggt gatgacgcta tggtgttcga
     6481 tggtctgcgt ctcatgaaga acggttacgg tgagcaggtg agtaaatggt ttaatcggac
     6541 gttgttgcct aaagtcctcg tagaccgtag cggcttagcg tttcactcgt ttagacatac
     6601 agttgcgacc cagttaaaac aacatggggt cgagttggcg tatgcacagg cgatcatggg
     6661 gcatagttca gggtctatta cgtacgaccg ttatgctaaa gaggtcgagg ttgataggct
     6721 ggtcaatgtg atggctgatg tttataagga gacctagagg agaccatagt ctaggatgac
     6781 acttactaca gacctgtgtg gtcatgtttt tgactcatgg ttttctctac ttgtcaaggg
     6841 ataattaata cgactcac
//
LOCUS       DAJTNW010000147         6818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNW010000147 DAJTNW010000000
VERSION     DAJTNW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..154)
                     /locus_tag="OO179_003750"
     CDS             complement(<1..154)
                     /locus_tag="OO179_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR5533651.1"
                     /translation="MYGTCETLCRALAAKYSGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTV"
     gene            complement(166..387)
                     /locus_tag="OO179_003751"
     CDS             complement(166..387)
                     /locus_tag="OO179_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533652.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(388..1071)
                     /locus_tag="OO179_003752"
     CDS             complement(388..1071)
                     /locus_tag="OO179_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5533653.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(1135..1446)
                     /locus_tag="OO179_003753"
     CDS             complement(1135..1446)
                     /locus_tag="OO179_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533654.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(1443..2345)
                     /locus_tag="OO179_003754"
     CDS             complement(1443..2345)
                     /locus_tag="OO179_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5533655.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            2785..3744
                     /locus_tag="OO179_003755"
     CDS             2785..3744
                     /locus_tag="OO179_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5533656.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            3744..4139
                     /locus_tag="OO179_003756"
     CDS             3744..4139
                     /locus_tag="OO179_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5533657.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            4322..4702
                     /locus_tag="OO179_003757"
     CDS             4322..4702
                     /locus_tag="OO179_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5533658.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQHITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            4748..5592
                     /locus_tag="OO179_003758"
     CDS             join(4748..5090,5090..5592)
                     /locus_tag="OO179_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5533659.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            complement(5762..6511)
                     /locus_tag="OO179_003759"
     CDS             complement(5762..6511)
                     /locus_tag="OO179_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5533660.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT         1967 a         1648 c         1581 g         1622 t
ORIGIN      
        1 ggacggtcct gatttcatga tcggacaggg aaatatccat tccgtcagcg agggcctgaa
       61 tctcttccgg ggaccagata accagcatca gcggcgtgtc tcccgaatac tttgctgcca
      121 gcgcccggca tagcgtttcg catgttccgt acatactctc tcccctcaat ctttgaagca
      181 ggaatccagt tccagaattt cggtatatgc ctccatcaag aggcggtttg cgcgaaacaa
      241 cgtctgacgc accagacgtt ccggcagggt gtcagcatta aagctgtacc gccgctccat
      301 ctcattcatg gttgtacgca ggtcgcacat ggcattggcc acctccgcca cttcggcgcg
      361 aagtttttcg tgtccggcat agttcattta cgcagcctcc ggcataaacc agtcatcatt
      421 cgcggccccc tgctgcatgg cccgctgtac ggcagccagt cgttgctgtc cggcacggtg
      481 atactgctca agcagctcga taccgatata ccggcgtccg gactggaggg cggcgacgca
      541 ggttgaaccg ctgcctgcaa acgggtccag cactatcgca ttcgggtgtg tgaagctctc
      601 aatcagcggt tgcaggctgg taacgggctt ttccgtcggg tgatggcggt tgcccgaata
      661 tttccagccc aacacgtccg gcagcgggtt ttgtggcaga cgtggacggc cttttgccag
      721 gatgtaggcg cattcgtggc gatagcccac atatgcggcc ttcgatgtgt agtttttggt
      781 gaagaccagg tgaccaacaa cgctgaatcc cgcatttttc caggcagaca taaagcgatc
      841 gacgcggttc cagccataga agctcaccat cagcgcgtct tttttgagta cgcggtacat
      901 ttcattacag gccggttgca gccattcatc agttttatca ccagcaatgg tacgcccctg
      961 acggtcacga aaaccgacga gatatggcgg atcggtgagg atgaaatcaa tggcgttatc
     1021 aggaatacgg gccataacat cgatgcagtt accgaggaca aaacgggaca taacagatcc
     1081 ttctcttaca gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg ctgttcatcg
     1141 cttccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt ttgacagcaa
     1201 ggttatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgacg ggctggcgta
     1261 cactgtcacc gacgccagcc gggagcggta aaagcgcccg tcccgtgccc gccggacgcg
     1321 ggacactgac cacaggcagc gtttatgccg cgccgcgtca gccggcgcag taaaaagagg
     1381 agcacggcga catcctttgt cggaaaagaa acgggcggtt gcccgcccgt ctccgtcata
     1441 cttcatccgc cataatgcgc cacattgtcg actatccacg caggtccggt gacttccggc
     1501 tgctcatcgt catctgtcgg ggaagaccat tcaagttccc cccacagcac atcgacgagc
     1561 tctccacgct catacaactg ccagccacag aaatcacagc cctgttcggc ataccagtgc
     1621 tccagcgtgc agctgaaacg gcggcttaat tctgcaataa cgtcgctttc cggctgacac
     1681 cacggagtgt cgaaatccac ctgaatgaag ttttctccct gactgctgat attcaggtca
     1741 tatccacaag gccacttcac gccataccgt tccgtatacc agtgatatgc agaaggaaca
     1801 ccgttcagaa caccaccatt aaagccgttc acttcaacat ccaggcgggt cggcagaacc
     1861 gtcagcaggt caaacggcat ggttttttcc ggcactacgt tgtcacacag acggttccac
     1921 cacaccgata catcctcgtt accccatatg tcacaccagt cacctctttt tgcggtgaaa
     1981 tgcacagtga tggtttccct ggcatcatcc ggtaatcctt cccaacgacg ctggccagta
     2041 ccgctctgta accagagttc atgcagcatc aggcagtttt gttcatccag caacacaccg
     2101 ttctgcaggt gtgtcagcca gcgggtgaac gcgatatttt ccgtcgatac agcaccacgt
     2161 ccggcagccg tcactccggg gcactgttca gagcggatat tttcagtgat ctgcagaagt
     2221 ccggcacttc cggccagaaa cagctgaata ccttcatttg tggcgcggcg ataaagcggt
     2281 gtgactgcac cgctggccag tcgtttaatc tcagcgatct gtgccggttc accaggaaaa
     2341 tacatacgat tcgagcacca gtttggcatg ggaattctcc tctcacatgc ggcctccatc
     2401 aggccgtgcc gcacctgtaa ccgggcgacc gcatacgaat gcaggtgcgg ccccgaagcc
     2461 gcagcgcgtg gaggaggaat gacgaaacgt gaccgggtga ggaggccggc gacagcgaca
     2521 cacttgcatc ggatgcagcc cggttaacgt gccggcacgg cctggttaaa tagtatccgt
     2581 ctcagttcgt ggcgcaaaat gagaaaccaa taagaactca aaatgaacca caacaagaac
     2641 ataaagagac tctaaaagag caacaccgtt tgacatagac tcaaaatgag tcacaataag
     2701 aaacaataaa aaactcaaaa agatgcaccg acataaagga tgctcaattt gagataaaca
     2761 caaactcaaa acgaggcaaa aagaatgcta aaagtatcat gcgatgatgg ttcaactaac
     2821 gtaaaactgg cctggcttga agatggcgag gtaagaacca gtctgtcagg aaacagcttt
     2881 aaagaaggct ggaacccggg actgtttaat gccggcaaag tttacaatta cgtcgtggat
     2941 gaaaaaaaat acacctatga tttgggaagc actgctgtca taggtacaac acatgtcagt
     3001 tatcaataca gcaccaccaa tctgcttgcc atacatcatg cactgctgac atccggcctt
     3061 cagccacagg atgtagaact gacagtaaca ttacctgtga ccgaattctt tgataatgac
     3121 aatcaaccaa atgaagaaag aatagaaaga aaaaaggcaa atgttctccg tgagatatct
     3181 ctgaataaag gggaaacatt taaaattaaa aaagttaatg taatgcctga atctttgcca
     3241 gctgcatttg aatcactaaa aaaagataaa gtaaacaaac ttgaacgcag cctgattatc
     3301 gatcttggag gaaccactct ggattgtggt ttaattctcg gtgcatttga aggcatttct
     3361 gaaatccgtg gctattcaga aatcgggaca tcccgcatca ctcataccgt catgaatgcg
     3421 ctgacgaaag cctccacacc atgtaattat tttattgctg atgagttaat aaaaaacaga
     3481 cacgataatg aatatctcca gacattaata aatgatgtag ctgaaataaa aaatatttct
     3541 catgtaatag acagagaagt gaaatctctg gcagaaagca tcaggcagga aatttcaaca
     3601 ttctcaggaa tgaaccgaat ctatctcact ggcggaggag ccgagcttat ttaccctcac
     3661 ataaagcaat atttcccgaa tctgaaagtg aacaaggtgg atgaacctca gtttgcattg
     3721 gtgaaagcaa tggtgcatgc gtaatggaaa gcagtgatcc aaaaaaaaga aagaaagttg
     3781 tggcatacct gcacccggca ctttatccgc aggacaacct gacacaacaa acaatagact
     3841 ctctcccagt acaaatgcgg ggagatttct acagacaatc ccttatttgc ggagcagctc
     3901 tttattctgt tgccccccga ctgttaacgc taatcagcgt ttttttcagt gagaaaatca
     3961 ctgcagaaaa tctggtgaaa cttatagaac aaacaacagg atacacatcc acatcaattg
     4021 atataagtgt gttaaaaaac atcatagagg catcttcaga aaacaaatca gagagcatca
     4081 cttcaaaaga tgatttcgaa gaacaaacca gacgcaatct ttctatgtta aaaaaataat
     4141 aaaagaagag caggttcagt caattacctg ctcttctttt atacatacta aaaaatcaga
     4201 tttttacgaa tattcagatt atcataaaaa atgcaacaga caggaatgca ataattttgc
     4261 attatctcgt tgcttgtaat gcaaatatgc attattgtca gtgtgtgaca ataacagaga
     4321 gatgacgatg gcgaccatta acgcacgtat tgatgatgat ataaagaacc aagcagatga
     4381 agtgctgaag ctgatgaata tcagtcaaac tcaggctatt gcagcttttt atcagcatat
     4441 tactgaacaa aagaaactgc catttgtgat cacctcaatt gtaaaaacgc cgcatgattt
     4501 attacgtgaa agtacagaca tgttagctga agctcttgca gtgatttcca atctacaggt
     4561 gtggactgaa cagcaggatg ggattggaaa agcaaagcta atggagtatt accgccgcct
     4621 cgacgcactg tactgttgtg caaaagagaa aataggtctt ctttcagata accgggatgc
     4681 tgaactaggc tgtgtccctt aatcatctga gttatagtaa ctgtctgttt acacaacata
     4741 ttgaactatg gctcgctacg accttcccga tgaggcatgg accatcatca agcccctgtt
     4801 acctcctgaa cccgccacac cacgggccgg acgcccatgg gctgagcacc gtaaaatcat
     4861 caatggcatg ttctgggtgt tatgttccgg tgcaccatgg cgcgatttac ccgaacgata
     4921 tggggcatgg aaaactgttt ataaccgctt taaccgatgg tcaaagtcag gcgtgattaa
     4981 tattattttc aacaggttgc tttcgctact tgatgcaaac ggctttattg actggtctgc
     5041 caccgcgctg gatggcagta atatccgggc gctgaaatgt gccgccggtg ctcaaaaaaa
     5101 catcccgata tcgacggaga taatgggccg ggtcgctctc gcggcggttt tggcaccaaa
     5161 atccatctgg caacagacgg aagtggcctc ccgttaaata tcgtgctaag ccccggacaa
     5221 gctcatgaaa gccagttcgc gcaacgactt ctggacggga ttggcgttca gcgtcagaac
     5281 ggcagcatga aacgccgtgg acatgcggta ctggctgata aagcgtattc agggcgcgca
     5341 ttgcgcaacg agctgaaaaa taacggtata aaggcagtaa tcccccgaaa gtcaaatgag
     5401 aaaatggcat cggatggacg tgcacagctt gatcgtgatg cgtactgcaa ccgtaatgtc
     5461 gttgaacggt gctttgggcg gctgaaagaa tatcgccgca tcgccacgcg ttacgacaaa
     5521 acggcgagaa attacctggc gatggtgaaa ctgggctgca tccgactctt ttatcaacgc
     5581 ttacgtaatt aagggacaca gcctagtgaa attaccgaca agaagggaaa gggcctttcc
     5641 gagagtggta aaggagaggc cctggaaata ccccacagcc ctgaaaaaga gccagtcagt
     5701 tgcttaactg actggcatta gccctgaagg tcggggttat aaggcgtact ggctaaaagc
     5761 attatttttt ctgattgtta gcgaattcat ctttcgcttt ttgtaattgt gcctggaaca
     5821 caggatttga gtgcagtgtt gctacaatag cagagccaac aatacgtgct gcgtcaacat
     5881 cactttgcca gtgataaccg caaataaccc tgctgtcccc cagttcataa ccccgtttca
     5941 gaatggtatc ctggcgtgcc ggatttattt cggacaatac aagtgctgtt gcccaaccaa
     6001 tcgatgtatg acctgatgga taagagccat ttctggataa tgtatcctgt tcttttgtat
     6061 tacacgtaga aacaccataa aaagcaaaag gtcgaatacg catataatat tcttttgctg
     6121 aacgcgttgc caggtctccg gcatcctcaa tcatatttgt cagtaattta tgcaattctg
     6181 gtgaatcttt agccgttatc ggagaaccaa aagcagcaga aaatacattt gccacgccac
     6241 cactactgag attggcatcc tcagctgcca gcttccctct ttcagtattt cgcaataaac
     6301 gccccttttc atacatggcc tggtcattca ggaaggcaat acttccgatt tgtggtggag
     6361 gcggtaataa tgccagactg tcaatagcat tatcgtttgt caggtagtaa aggtcaggtt
     6421 ttgttgtcac atcatttccc ggagggaatg atgcaaaggt atttaaagaa aataccccga
     6481 caatactaag agtaaaaagc tgtcttttca tataacatct ccttatagag gataaatatt
     6541 tataattatt ttattaagag tataaagtac tggataataa aattgtgtgt gctatcacat
     6601 aatttaaata aatcagaaga tgctaaaaca aaggttacct attaaatatc cgttatgaac
     6661 aagcataaaa aggaggacgt ttaaaacacc cagaacaaac agttagaatt aagttcttac
     6721 caacaagggg aataagcaac ttgtattttt gagttgcttt tacagacaat gactccaatt
     6781 agtagaacat atgtttttcg ataaacgcac cgataact
//
LOCUS       DAJTNW010000148         6670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNW010000148 DAJTNW010000000
VERSION     DAJTNW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(152..2155)
                     /locus_tag="OO179_003760"
     CDS             complement(152..2155)
                     /locus_tag="OO179_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533661.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2152..3288)
                     /locus_tag="OO179_003761"
     CDS             complement(2152..3288)
                     /locus_tag="OO179_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5533662.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3281..5560)
                     /locus_tag="OO179_003762"
     CDS             complement(3281..5560)
                     /locus_tag="OO179_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533663.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5571..6659)
                     /locus_tag="OO179_003763"
     CDS             complement(5571..6659)
                     /locus_tag="OO179_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5533664.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1530 a         1834 c         1787 g         1519 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat taccaactgg
       61 tgactctcac caggggctat ataggatatg cctaataccg tggcgtgagc agtccggaac
      121 tggagtagaa ctcttagtaa aaagcactat ttcatccttg ttgctgaagc atggggaata
      181 attgttcgca aagcaaaaca ccgttattca ttgcttctac ccgtgcctcg ctttctgtat
      241 tacgaaattg tgccaacaca tgtgccagcc gataaaaacc caccgcggtg aggtcattcg
      301 ccagcaactc tgcctgatta atagcgctct gttcctgata gcgccagccg ttatggagca
      361 gttgaataag taacgcctgg cagcgcatca gcaactgatg agcagtagac ggcacaggca
      421 gaacgctggc agaagatagt gataccacca caggcgcagt ttctgcgtcc agcgcccagg
      481 cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa ttgcccatca acaaaactga
      541 caaagccaga aaccagacac acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt
      601 caatggcata aggcgcgctg gctgaggctg gcagtgataa cgtcagcaga ttatcttcac
      661 cttcgccgct gattacctgc gcatccagcg tctggcggct gctatcccaa ccgagcgaaa
      721 tacactcagc gaccggcaga ataaataagt tatcgacctg attaagaggc cgtatgcagg
      781 cggggggacg ctggcgtaaa tattcccgca aagccacaat gcccggctgg cgtaacggcg
      841 cgctcaacat ttgccaggca tcaggcgaca gcggtacaac gctgcttaag cggttgcggg
      901 tagctaacag cagctcgcca tcggcactgc gttttgctgc ttgtgaaaca atttgcccac
      961 ccgccagtgc gccagcctga aaactaaaca gccgacgcgt agctgccggt gagtcttcct
     1021 gttcacttcg cggccaactg cgcgaaaggt gcaaaatact gccggtgtcg ggatcggtaa
     1081 accagatgcg taaaccataa tgctcaatat cctgccagca acgcatacct aaagacacca
     1141 gccgcagatg atcaagcttt gcttctccgg caatgccaga gccaacgacc gtgcgccacg
     1201 gcacaggagg aacttcacca acactgtcgc tccgggccat ctcttgtgcg caatttaatc
     1261 gactgtttaa tgccgcaagc tgacgtaagc attctccggc atgatagtgg ctggcgcggg
     1321 cgtggaaggc atcaacgctg gcgcgcagtt gccgtagcga ttcactcacc catcgccagc
     1381 tgcagcgttc cgctgcttgc tgcgcgcggc tgaaagcggc ctcgtagtga ataagcggct
     1441 ggctgatgcc gctcagccat aatgcctggc ttagttgttg aacatattga cgacacgttt
     1501 tgccttcttc gctggcaaac ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc
     1561 agattaaatg agtcaattcc gcttgctgcg cttttgcctg gacaaaggcc tgtactgcca
     1621 gtacgacatg ttcgcaaagt gtgccttcaa tacaatcaca acgggcgaaa cgaatactgc
     1681 tgcgggaata aaaacgcaca tcgctcatcg gtaagcgggc agagggaatt tcacccggcg
     1741 cacagaacaa ctcaatggtg atgcttttag cgaccagcgc ctgtgcgcgt ttgcgggtgg
     1801 catcgggaag ggtagccagt tcttccagcc agattgccgg atcccattcc tcttcttttc
     1861 ccgtaggctg ggcggtagca caaagtcgtt gataacttaa caccagcatc acgcgatgac
     1921 ggcacatgcc gctggcccca taggtgcact gagcctcttt cagtgcctgg ccgttcgcca
     1981 gctgggtacg gacaccgtca ctgaaggtgg cgattaaagc gctgttctca tggctgattt
     2041 ccgggacgtt gccattttcc agttccttaa ggctgcgctt aacaaaaccg gcattgctta
     2101 acgccgtcag tgcctgcggt gtcagttcta ataattccgg acgtagtgaa ttcatgactg
     2161 aagattctcc gcaagccatg atgccagctc gcccggtgtc atggcggcta tttgtgcgcc
     2221 gacattaatc agcgcctggg ccgtatcgcg gtcatagcaa ggtgttgctg tgctatcgag
     2281 cgctgccagt cccagcactt tgatgccgct ctggacacac tttttcactt ggtgcgtcag
     2341 caatgatgat gaaccccctt cataaaaatc gctcacgagg ataatgacgc tttttgcagg
     2401 ttgttcaata agttgccgac catactccat ggcactggcg atattggttc cgccgcctaa
     2461 ttgcactttc attaataact ctaccggatc ggcaacgtct gccgtgagat caacgacgct
     2521 tgtgtcaaac gccaccagat gggtacgaat gccgggtaac tgccataaac aggccgccat
     2581 caccgcagag tggatcaccg aatcgaccat cgatccgctt tgatcaacca gtaagaccag
     2641 ttgccattgc tcgctttggc gtttaatgcg gctgttaaag cgggggaatt cgatatacaa
     2701 cttgccgtgt tgcgggtgcc agtgttgcag gttggcgcgc agagtacttt tgaaatcaaa
     2761 gtttcgcgcc agtggaataa atgagcggcg acgacgatcg cggacaccag aaaaagcctg
     2821 acgaacttcc tttgccagtc gcgccataat ttcttccaca acctggcgca caatcctgcg
     2881 ggcggcagcc agtacttcgg ggttcatcag atgtttggtg tgcaaaacgg cgcgtagcag
     2941 gctttcggaa ggctgcatac gttccagcac gtcgagattt gtcaccacat cttcaatgcc
     3001 gtagcgcagt acggcatcgc tttccagccg ctcaatcacc tgttgcggaa acagcgtgtg
     3061 aatactgttg atccactcag gagtggtgag atttgagcca cctaatccac tagagcgttc
     3121 accacgctgg agccgttcag gatcgcgccc atacagccac tccagcgcgt gatctatctg
     3181 ccgggcgttg tcatccagcc cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg
     3241 ccagcgttgt agctcacggg tggtcagaag atcgttcagt tcagacatgg aaaactcccc
     3301 agtgttgcag cgatgccagt gcctgctgtt cgagttgttg atgatgtgca atggcttgcg
     3361 gtggacaatg caacggcatt tgcagtgccg aaacgggaag ttgcgccagt tgataatgct
     3421 caagcacctg atgcgccagc gtcccgcgtt ctcgtggcgg tagccaggcc attgccgcgc
     3481 gtaaatcggg cagggcattg gtaaaatcgg catcacttag ttgatttaaa tgactgctga
     3541 aaccggcgat aaatgccggc tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt
     3601 gcagcgcctc gccggattgt gccggggata actgcgccag catcgtcagc gccgcttcgg
     3661 cactagcgtt gggatgttcc agacgcatca gcgcaccaag agtcgcgccg cgatccagtg
     3721 ccaggacatg aatcgcctga ctgtgtcgct ccagcaaggc taccgccgca gaaagcgaaa
     3781 cgccggggag ttgcacaccg ctatgtagat cgcgcaggat atgacacaac gcttgccagc
     3841 tatgcaagtg agcgtgaaac tccttttcat ccggtctgcc gttggattca cacagccaca
     3901 gcgtgcgatc gatagccgcg cataacgtcg tttgtaatat ctgcgcgcct tgcataccgc
     3961 taatttcatc cagccgccat aaggcatata gcacttccag cgcctggccc atttcggcaa
     4021 attgattttc ctgggcgatt aataatgtga gttgctccag taattgttga ctgaaggacg
     4081 ccagacccgc tgacgccgct tggctaagac atgtggtaat gctgccgatt ccacccgcgt
     4141 ccagcatatc ggcttctaat ttatggcgtg cggcttcctg gagtgtggca ccaaagcagg
     4201 cggcctcaat caatgccgca tgctggctaa gcgggcgggt taatttccag cgttcttcac
     4261 cgttgcctgc aagtgtcagt gtgcttccct gctggcgtac aatccctggg atctccagta
     4321 ttgccagccg atgtaacacc tgactttgcg ctagtccatt ggggttaaag cgatttaagg
     4381 taagctccgc cggaagagag attgcggtac gttccagttc gcaagtgaca tcttttggca
     4441 gaggcggttg tggcgtagaa ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta
     4501 tcaacgttag cagaatcgga tcggtatcgg gatgaataac gccgcgatag ctccacggca
     4561 acggcgcatt cagggcttct ttaatcagcg atcctgctat ggcatccagc cagtcagtgc
     4621 gtaacggtag tgtatgaccg cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag
     4681 ccatatccgc ggtcgaagca ggcaacttgt gctggcgcaa acgggtgaga accgttttta
     4741 gcagttgttc tcccgcctgc tgtaagcccc actgccagca ccagttttgc cagaccgggg
     4801 caggcattcc tgaaaggtat cctgccagca catcaaggcg cttttcactg tagggggtga
     4861 gataacaacc tgtaacggca tctgccagcg acggcaattc tggcgtgtta attacctgcg
     4921 gacattcgcg ccacattttt gccagtgccg gagcgtgcca gccaccgcag acaactaaca
     4981 cgtcgccatt attttgctgc atcgcccatc caatccagcg ggccataaag gcttcgcgct
     5041 gacgatttag agcatcgccg ggggagtcgc cccgcagttg ggcaaaatag tgcgccagtg
     5101 cagagggtaa tgcagtttgc tggctttcat cttcgaacaa gtgatcccac agggtgtcgc
     5161 tgttatccat gcgggtggca cgcagcagca aggtctggct ttcctcttgc gtatcaggcg
     5221 aatcgtcctc ttcttcactc tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct
     5281 gaatacgacg atcggcttgt agcgcctgcc actccggcga aaattcagca aatggcgtcc
     5341 aggcaccacg cccgggggct gcaccgtcct gatactggca ataactgtaa atagctaccg
     5401 gaagctggtg ggctaaaaac agttcgtcta cccggtcatt aaaatcagca gggccttcaa
     5461 tcaacacgta tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg
     5521 gactatgatg ccggatgccg acaattaacg gctcgctcat gcatctttcc tcacggcagg
     5581 cggtggcggg cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt
     5641 tcgaagtaac ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca
     5701 atgcagtcca ccaggtcggc tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg
     5761 cctacggcgt gggccacgtt cacggcttca gcggtggaca taatcgctgt tagtgtatcc
     5821 attgacgttt tcttttcgcc gttggcacgc agatcgcgga aggtgcggac cagtaactcc
     5881 agtaccgcgt caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca
     5941 ctggcgacaa gctccagctc ctgggcgaaa tccataatcg gaaagacagt ttcaaaatcg
     6001 aagcgacgct ttagcgcagc actcatttca ttgacgccgc gatcgcgggt attggcagtg
     6061 gcaataatat taaagccttc tcgcgcatag agctgactgg cttcaccagt gagttccggc
     6121 accgtcatca ccctgtcaga aagcatacct aacagacagt cttgcacttc cagcggtgta
     6181 cgcgttatct cttcaaaacg gacgattttg ccgtcgcgca ttccctggta aagcggggca
     6241 gggaccagcg cttccgttga tggaccatga ttgataagca gggcgtagtt ccagccgtac
     6301 ttgatttgat cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta
     6361 atagaggttg ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac
     6421 atcaaaccgc gtccggtggc aagagtgact aacatacgtt cgacagcgga gggactaatc
     6481 accactttac ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcaggc
     6541 aaacttaact gccaaccggg cgggcaaggt gcgttatcat tttgttttaa ttttgccagt
     6601 tcatcggcgt ataacacagc agcaggcgga cgctgcagat gattattctg cggtgacata
     6661 tattcatccc
//
LOCUS       DAJTNW010000149         6519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNW010000149 DAJTNW010000000
VERSION     DAJTNW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..258)
                     /locus_tag="OO179_003764"
     CDS             complement(<1..258)
                     /locus_tag="OO179_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5533665.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQ"
     gene            complement(258..623)
                     /locus_tag="OO179_003765"
     CDS             complement(258..623)
                     /locus_tag="OO179_003765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533666.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            712..1059
                     /locus_tag="OO179_003766"
                     /pseudo
     CDS             712..1059
                     /locus_tag="OO179_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            1049..1843
                     /locus_tag="OO179_003767"
     CDS             1049..1843
                     /locus_tag="OO179_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5533667.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1865..3289
                     /gene="patD"
                     /locus_tag="OO179_003768"
     CDS             1865..3289
                     /gene="patD"
                     /locus_tag="OO179_003768"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5533668.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            3499..4308
                     /locus_tag="OO179_003769"
     CDS             3499..4308
                     /locus_tag="OO179_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5533669.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(4710..4805)
                     /gene="yncL"
                     /locus_tag="OO179_003770"
     CDS             complement(4710..4805)
                     /gene="yncL"
                     /locus_tag="OO179_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5533670.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            5000..5173
                     /gene="ortT"
                     /locus_tag="OO179_003771"
     CDS             5000..5173
                     /gene="ortT"
                     /locus_tag="OO179_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5533671.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            5259..5492
                     /locus_tag="OO179_003772"
     CDS             5259..5492
                     /locus_tag="OO179_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5533672.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(5493..5942)
                     /locus_tag="OO179_003773"
     CDS             complement(5493..5942)
                     /locus_tag="OO179_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5533673.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(5939..6457)
                     /gene="mddA"
                     /locus_tag="OO179_003774"
     CDS             complement(5939..6457)
                     /gene="mddA"
                     /locus_tag="OO179_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5533674.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
BASE COUNT         1695 a         1488 c         1651 g         1685 t
ORIGIN      
        1 ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg caggagcggc ttttacaccg
       61 gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa caacaccact gcgtgtagcc
      121 catcagtgtg gtcccgcagg ccatgatttt tgtcactgat tcaattacca ccggacggac
      181 tgccccttcc ggttgtttct ccagccagtt aagccagcgg tttccctgct gaaaaatatc
      241 ggcaaaacgg gggagcatca gaagggcggg gcgactccgt ccggccagtg aaccgtgcca
      301 cactccgggc agtacatacc gccggcgctg ataccggcaa gaatggtcgc aaattcccgc
      361 tccgtgcagc gggcgatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
      421 agctttttgt ttcccgcatc cacaaagaac ggaatattca ggtctgcgca gcattcaacg
      481 gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct
      541 tcgtgaagct tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta
      601 cggggtttag ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta
      661 taacggtaat tgcagaccat gacaagccgc agccgtcagg ctgcctactc gatgatcctg
      721 cccgttcagg cggcgcttga gcgtttgccg ccgtcattgt tgcaggcgtc ggctgatctc
      781 ggcgcacgtc cacgacaaac ctttcgctat gtggtgctgc cgctggcaat cccgggtatt
      841 gccgctggct ctatctttac cttctcactc acactgggcg attttatcgt cccgcagctg
      901 gttggtcctc caggatattt tatcggcaat atggtttatt cccagcaggg ggcgattggc
      961 aatatgccga tggcggcggc attcaccctg gtgccgatta ttctcatcgc actgtaccta
     1021 gcgttcgtga aacgtctggg agcgttcgat gcactctgaa cgcgcaccgt ttttcctcaa
     1081 actggcggcc tggggcggcg ttgttttcct acattttccc atcctgataa tcgccgccta
     1141 tgcgtttaac actgaagatg cggcgtttag ttttccaccg cagggcctga cgctgcgctg
     1201 gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc gtgacgctgt cacttaaagt
     1261 ggcggcgctg gcgacattaa ttgcgctggt gttagggacg ctggcagctg ccgcgctgtg
     1321 gagacgagac tttttcggca aaaacgccat ttcgctgtta ttgctgctgc ccattgcgct
     1381 gccgggcatt gtcactggtc tggcgctatt aaccgccttt aaaaccatca atctggagcc
     1441 gggatttttc accatcgtgg tcggtcatgc gactttttgc gtagtggtgg tgtttaacaa
     1501 tgtcatcgcc cgttttcgcc gcacctcgtg gagtctggtt gaggcgtcaa tggatcttgg
     1561 ggccaatggc tggcaaacct tccgctacgt agtgttgccg aatctcagtt cggcgttact
     1621 ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa atcatcgtta cgacctttac
     1681 ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat cagcttgggc gaccgcgtga
     1741 tgtaccggta accaacgtgg tggcactgct ggttatgttg gtaacaacct tgccgatcct
     1801 gggggcctgg tggctaaccc gcgaaggcga caatggtcaa taaccactga tacaggaata
     1861 agctatgcaa cataagttac tgattaacgg agaacgggtt agcggcgaag gggaaaaaca
     1921 gcctgtctat aatccggcaa cgggggacgt tttactggag attgccgagg tatccgcaga
     1981 gcaggtcgat gctgctgtgc gtgcggcaga tgcagcattt gctgaatggg ggcaaaccac
     2041 accgaaagcg cgtgcggaat gcctgctgaa actggcggat gttatcgaag aaaatggtca
     2101 ggtttttgcc gaactggaat cccgtaattg tggcaaaccg ctgcatagtg cgttcaatga
     2161 tgaaatcccg gcgattgtcg atgtttttcg ctttttcgcg ggtgcggcgc gctgtctgaa
     2221 tggtctggcg gcaggtgaat atcttgaagg tcatacttcg atgatccgtc gcgatccgtt
     2281 gggggtcgtg gcttctatcg caccgtggaa ttatccgctg atgatggccg cgtggaaact
     2341 tgctccggcg ctggcggcag ggaactgcgt agtgcttaaa ccatcagaaa ttaccccgct
     2401 gaccgcgttg aagttggcag agctggcgaa agatatcttc ccggcaggcg tgattaacat
     2461 actgtttggc agaggcaaaa cggtgggtga tccgctgacc ggtcatccca aagtgcggat
     2521 ggtgtcgctg acgggctcta tcgccaccgg cgagcacatc atcagccata ccgcgtcgtc
     2581 cattaagcgt actcatatgg aacttggtgg caaagcgcca gtgattgttt ttgatgatgc
     2641 ggatattgaa gcagtggtcg aaggtgtacg tacatttggc tattacaatg ctggacagga
     2701 ttgtactgcg gcttgtcgga tctacgcgca aaaaggcatt tacgatacgc tggtggaaaa
     2761 actgggtgct gcggtggcaa cgttaaaatc tggtgcgccg gatgacgagt ctacggagct
     2821 tggaccttta agctcgctgg cgcatctcga acgcgtcagc aaggcagtag aagaggcgaa
     2881 agcgacaggg cacatcaaag tgatcactgg cggtgaaaag cgcaagggta atggctatta
     2941 ctatgcgccg acgctgctgg ctggcgcatt acaggacgat gccatcgtgc aaaaagaggt
     3001 atttggtcca gtagtgagtg ttacgccctt cgacaacgaa gaacaggtgg tgaactgggc
     3061 gaatgacagc cagtacggac ttgcatcttc ggtatggacg aaagatgtgg gcagggcgca
     3121 tcgcgtcagc gcacggctgc aatatggttg tacctgggtc aatacccatt tcatgctggt
     3181 aagtgaaatg ccgcacggtg ggcagaaact ttctggttac ggcaaggata tgtcacttta
     3241 tgggctggag gattacaccg tcgtccgcca cgtcatggtt aaacattaag ttagatgtta
     3301 gtggctgtta taggacggcc actatcgttt taggttctga gtttaatggc ggttaagtat
     3361 tagaatgatt acattaataa ctggcgtaaa aattaggcta aatgtttgat ggcgattttt
     3421 catcctgtca cagttgatta gcataaggct ttagagtatc gttattaaac taatacttta
     3481 taagtagaag tattaacaat gaaagttcaa ttgtacaaat tcacggaaga taaaaataaa
     3541 acccttacct tcagatggac gaaaaaacac tttgagtttt gtatggataa taaaatcttt
     3601 ttaaatcata aggggaagaa gtcgtataaa gaaaggaatt tatttctttt ctcaaaaggt
     3661 gacaagataa caattgaaga taatgttatt gctgaagaat attcaacaat gcctgtgaaa
     3721 aatttctcca gtgtcggcgc tttttcattt ccgacctgcc atttttcagg gaatatacgt
     3781 ataggtcgat tttgtagtat tgcatcaaat gtaaaaataa tgggtggaaa tcatcctttg
     3841 aatcgtttta ctactcacat gatgacttat aacggtgaat ttgataagtt tgctatgagt
     3901 gaatttgaac gtagctggac attgaaacca ttcattacca aacctgaaaa tccgattata
     3961 ggaaatgatg tctggatagg aaatgatgtt gtattgaaag gtggcatcgc aataggtgat
     4021 ggtgctgtta ttgctgccaa ttccgttgtt accaaagatg tgcccccata tgccatagtt
     4081 gcaggagttc ctgcaaaaat aatcagattt aggtttgatt caaatgttat tgatgaactg
     4141 ttgagaataa aatggtggaa ttataactac tcagatcttc cggataataa taagtgtgat
     4201 gatataaatt attttgttga agagatgaat cgattgatat caaatggaaa tattcaagag
     4261 agggactata aaaaattcaa tctttctgag gtattcagag gcctataatt atctgttttg
     4321 catgataaat gggcaagata accagggcgg ggtttgcaat ttcaatgggg cagcgtcatc
     4381 aaggaggttg caaacatcct gccatgcata tactttacgc catcttttta tatttacgtt
     4441 attccttgaa agtataaagc atttatagac tcccgcgcgt ataaagtatt cgtgatagaa
     4501 aaatgtgaaa acaagattcc gtttgaatca gtaagctgtt tgtatagacg acagtagggg
     4561 gatgttttct actcatttca atgtagatgc cagcaaaaag aagtaaatat taaggataat
     4621 gttgcaaact tggagagtat gagaagatct tgcgtactgt cagagagttg atttctctga
     4681 cagtacgtgc tgcgtgcaaa atcactacat caaataaacc agccaaatct catagagata
     4741 agagtaaaca aaccaacagc agcaaagaca tttatcagta ctacagttct actggaaaca
     4801 ttcatttttg ccacctttta tcattgcccc tatgtgtata gtacgggtta agaaaatccg
     4861 caataatact cattctgaag atggataagg gcaagttgct gtttgatgat tttttctcaa
     4921 actggtgaaa aaacttgatg cacgtcaaaa aatggcgcat atttgcgcgc gttttattca
     4981 tatggctgga cgcccgtaca tgtctctcta tcaacatatg cttgtttttt atgcggttat
     5041 ggcagcaatc gcatttctta tcacctggtt tctttctcac gataagaaac gcatccgttt
     5101 cttaagcgcc tttctggtgg gggccacatg gccgatgagt ttcccggtgg cgctgttgtt
     5161 ttcactgttt taaatcttac tcactggctt gacggcacca cgcagactta tatcatttgg
     5221 attaatcgat aaatttcaca agtggctaag gagaaagtat gtcgcatctg gatgaagtca
     5281 tcgcgcgcgt ggatgccgcg attgaagaga gcgtcattgc ccatatgaac gaattattaa
     5341 tcgccctgag cgatgacgcg gagttaagtc gggaagatcg ctacacccag cagcagcgtt
     5401 tgcgcaccgc tattgctcat cacggtcgca agcataaaga agatatggaa gcgcgccacg
     5461 aacagttaac caaaggcggc accatcctct gattaaaatg agcgtctggc caccaaccag
     5521 gcgccaacca caagtaaaat tgcgccacat attgggccga acaacgcccg tagtggtacg
     5581 ccatgactgc ggaagatatc cagcaccaga ccgccaatca gctggctggc gaccagtacc
     5641 gcaatagttg ttgccgctcc cacattctga tatccgctga tactggcgaa gacaaaaaac
     5701 gaacccagca agccaggtat aagtgtccac cagcgcacgc tggacaccag ttcgccaaac
     5761 cccgccatgc cttgtttaaa ccataaaata gaaacaaaga gcacaatccc caccagtgag
     5821 ttcagcaaca tggcgatgag aatggtagag gaggtctggg tgatgcgcgc catcagcgtg
     5881 ttctggacta ccagaccaat tccggcggca atcaaaaaag caagggtgag cgactgattc
     5941 atccaatcgc gtccggttca gtgcgctcgt cgagttgcaa ctgcataaat gtcagatcca
     6001 gccaatgacc aaatttagtg cctacctgcg gcatttgtgc ggtgacgaca aatcccagcg
     6061 actggtgcag atgcagcgaa gcctgatttt gcgattcgat cccggcgacc atgacatgct
     6121 tcccgcaatc ccgcgcttca tcaatcaatc gacttaacaa tttacgcccc agacctttgc
     6181 cctgatgatc gggatggaca taaaccgaat gttccacggt atggcgaaaa ccatcgaaac
     6241 tacgccagtc gccaaacgag gcatatcccg taactacgcc atcttcttca cttaccagca
     6301 ccggataacc tgctatggtc cgcgcttcaa accaggcaat gcggttatct gcatccaccg
     6361 tttggtcatt ccagatagcc gccgtatata acacggcgtg gttataaatt tccgcaatgg
     6421 cagcacagtc ggctttgcgg gcaaaacgga tggacatgtc tggcctcaat aaaataatga
     6481 tgatgatgtt atcaatacta tcgaaaacag cccgaagca
//
LOCUS       DAJTNW010000150         6374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNW010000150 DAJTNW010000000
VERSION     DAJTNW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="OO179_003775"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="OO179_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="OO179_003776"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="OO179_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OO179_003777"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OO179_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5533675.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OO179_003778"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OO179_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5533676.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OO179_003779"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OO179_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5533677.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="OO179_003780"
     CDS             complement(5701..>6374)
                     /locus_tag="OO179_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5533678.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1534 a         1680 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DAJTNW010000151         6332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNW010000151 DAJTNW010000000
VERSION     DAJTNW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            7..438
                     /locus_tag="OO179_003781"
     CDS             7..438
                     /locus_tag="OO179_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5533679.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO179_003782"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO179_003782"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5533680.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO179_003783"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO179_003783"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5533681.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO179_003784"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO179_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5533682.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OO179_003785"
     CDS             complement(4440..5147)
                     /locus_tag="OO179_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5533683.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO179_003786"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO179_003786"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5533684.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OO179_003787"
     CDS             complement(6005..6208)
                     /locus_tag="OO179_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5533685.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1406 a         1811 c         1648 g         1467 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccaccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg ct
//
LOCUS       DAJTNW010000152         6254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNW010000152 DAJTNW010000000
VERSION     DAJTNW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO179_003788"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO179_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5533686.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLCEEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO179_003789"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO179_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5533687.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO179_003790"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO179_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO179_003791"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO179_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1455 c         1477 g         1665 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgtgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DAJTNW010000153         6235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNW010000153 DAJTNW010000000
VERSION     DAJTNW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..656)
                     /locus_tag="OO179_003792"
     CDS             complement(<1..656)
                     /locus_tag="OO179_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR5533688.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     "
     gene            complement(646..2403)
                     /locus_tag="OO179_003793"
     CDS             complement(646..2403)
                     /locus_tag="OO179_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5533689.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2419..3303)
                     /locus_tag="OO179_003794"
     CDS             complement(2419..3303)
                     /locus_tag="OO179_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5533690.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3314..3919)
                     /locus_tag="OO179_003795"
     CDS             complement(3314..3919)
                     /locus_tag="OO179_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5533691.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4090..>6235)
                     /locus_tag="OO179_003796"
     CDS             complement(4090..>6235)
                     /locus_tag="OO179_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5533692.1"
                     /translation="NSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVK
                     GNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSIN
                     ARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDAL
                     KEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDL
                     AIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGL
                     LDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWL
                     LPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQ
                     LLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRD
                     MQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDA
                     YETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLKELQQ
                     NTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAK
                     SNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALEL
                     VNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1830 a         1563 c         1308 g         1534 t
ORIGIN      
        1 attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac gggcaccatc
       61 cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg atgctgccat
      121 gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt acgccacctc
      181 acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa
      241 ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcctgtcacc
      301 ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa accgcagcag
      361 gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa tgtcgagcta
      421 caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag
      481 ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc atggctgtta
      541 aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa aaacggggcc
      601 agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt tatcacgacg
      661 caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc atttatcaaa
      721 tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca ttacccacgg
      781 tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg
      841 ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac ctgcgagtga
      901 gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa tcgccagcgt
      961 aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg
     1021 cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa catttaactc
     1081 actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg catccagtac
     1141 atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tggcgtggag
     1201 atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc gaataccgcg
     1261 ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt tactgccttg
     1321 cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg tactcattcc
     1381 gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca taaagcgata
     1441 cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc atcgatccgc
     1501 tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt tagcatataa
     1561 gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt cacccaatgt
     1621 gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta atctctgata
     1681 aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca gaagttgcgt
     1741 ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat agagatcgag
     1801 caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac tcgccacccg
     1861 ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa atagaccacg
     1921 cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt taaccttaaa
     1981 ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa cccatgtggc
     2041 aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc cgacctggct
     2101 gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca taagagacgg
     2161 gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg cataaaaagc
     2221 agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat gttcatgccc
     2281 gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc agtgccgata
     2341 atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc
     2401 cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa ataccggagc
     2461 ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta atagttttcc
     2521 actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc
     2581 actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg gtgtcagcaa
     2641 cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca gaccttccag
     2701 cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa
     2761 atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca gtaacgcccc
     2821 gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca aggcaaaaac
     2881 ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg aggggtcacc
     2941 cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa ataacagaaa
     3001 cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc aatataacca
     3061 acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa aacttatcag
     3121 cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa aacaaatgac
     3181 gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct caggtaaccg
     3241 ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag tgttgccaga
     3301 gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa cgattaatcg
     3361 cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat tgcatccact
     3421 gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga tcacttttgc
     3481 caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact
     3541 cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt tccggtaaaa
     3601 ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca ttcaaaatcg
     3661 cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc gcattgagcg
     3721 ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt gccaccaaca
     3781 ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg tgattcgctg
     3841 taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga aagagcggtt
     3901 ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat ggtgcatcaa
     3961 ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat atatcaaggt
     4021 attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta aaaattatta
     4081 cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta ttgcgcgcgt
     4141 cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa gctctaacgc
     4201 cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc ggggattatt
     4261 catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag agggggtgtg
     4321 gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca gtgtactcca
     4381 ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat cggggtcgag
     4441 tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt cctgcctcag
     4501 aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct tgagaacaac
     4561 agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt cttccaaacg
     4621 tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc taatcagctc
     4681 agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg gattattttt
     4741 ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca gatactgatt
     4801 tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc gttgtaaagc
     4861 agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg attttttctc
     4921 catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata acacacgtat
     4981 tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt cactctcttg
     5041 tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga acgctaagct
     5101 tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt aagtgagttg
     5161 tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc catcaataat
     5221 ggattcgggc aataaccacg catctttata aagctcctga atgatcatat cccaatctct
     5281 tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa gattcaacgg
     5341 accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga gcggttcacg
     5401 gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca actccagaat
     5461 ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat tgctattaat
     5521 aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc gcattatggc
     5581 aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt catccagcat
     5641 ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt cttttttcat
     5701 ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc
     5761 atcttcatca tttattagac cccaataata tgcatcagga tttattatca tcagcgcttt
     5821 tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac tatttgtcga
     5881 agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat taatactccc
     5941 cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac ttgcatttat
     6001 cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca cgttaccgag
     6061 aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg tcaaattgcc
     6121 aagaacaatc aatttttgat tactctctat attaccttct accactaaat cgccacgatg
     6181 cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac tgttg
//
LOCUS       DAJTNW010000154         6233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNW010000154 DAJTNW010000000
VERSION     DAJTNW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO179_003797"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO179_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5533693.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO179_003798"
     CDS             2089..2238
                     /locus_tag="OO179_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533694.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3016..3456)
                     /locus_tag="OO179_003799"
     CDS             complement(3016..3456)
                     /locus_tag="OO179_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5533695.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3674..4063
                     /locus_tag="OO179_003800"
     CDS             3674..4063
                     /locus_tag="OO179_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533696.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4107..4511
                     /locus_tag="OO179_003801"
                     /pseudo
     CDS             4107..4511
                     /locus_tag="OO179_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4618..5454)
                     /locus_tag="OO179_003802"
     CDS             complement(4618..5454)
                     /locus_tag="OO179_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5533697.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5454..>6233)
                     /locus_tag="OO179_003803"
                     /note="aph(3'')-Ib"
     CDS             complement(5454..>6233)
                     /locus_tag="OO179_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5533698.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1441 a         1627 c         1642 g         1523 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc acttttacat
     2641 cattccttca gctcatacgt cactttcatc ctctgtgcca tttgtacatc acccccttcc
     2701 tgaacgtgct gacagcgggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     2761 attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttaccccgg
     2821 ctgaatggtg tgaatttccg cgctatgcac ttgtgcgcat actcatgcat gccgtaaaaa
     2881 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     2941 cgtcagaaac gccctgaggg cctctcaggc ggtgctgata acagatgtta tggtaaatca
     3001 gagtcacctg cactgttact ctgaaccgga gcgaaacagc cgcgaccaga atccccgctt
     3061 tttttcctgt gccagcgcgt cagccagacg gctaatttcg gcctgtagtt gttccctctc
     3121 ctcatttcgc aggcggtttt ctgcttcccg cgccatcttg tcttcaagca ttagggtaac
     3181 agtctgctgc agagcgtcca gtttctgctg aatgacgtct agctgagcat tttccgcctg
     3241 gctaacgaca gatttggagt gtccctcctt gtgccttttt ggtgtctcag aagtgcgcag
     3301 ctcgccatag gcccgaatca gttcactggt ctcgaactcc cggcgatcat tttttcctgt
     3361 gcgatagctg acaaggcctt tagccatgtc acgatataat tgcgagcgcg actttcctgt
     3421 taatttcagt gtctcttgaa ctgtgtgcca tgccataaaa gtgacagtgt ctcctgtgtg
     3481 taccgtattg taacagcaga acatattact gttttaacac aaaaggtctg aggctgcccg
     3541 tgaggcgtga aaaatacaca aaaaacctgt tgacggtgtg gttttgacgg gcgtagtatt
     3601 tcgaatactg tatataaaca cattgttata aatacagttt ttgctgtttt taaaagtcaa
     3661 agaggataat tttatgagta cagagttaaa tagcggtact gtcggtggtt ttggtcacat
     3721 gggaccaaat gacccctaca atgatggtca tagcggctct ggaagttcgg gaggtagttt
     3781 taacggtggt ggtaatggtg gccatgataa tagtagtggt tcatcttata ctgcatcctt
     3841 tggtctcatc actggccgac acccgataac tgtcccaggt acgaagaata ttaattctgt
     3901 cgatctttct gctgtcatta ataacattat acatcatgaa ggtgcacgca ataacatgta
     3961 cctagatact gcgaacaatg taagagtggg agtgacgggc actggctggc aatgtctagc
     4021 aacggcaggc atttcggctg agggtaaaag aactttccgc taagcgatag actgtatgta
     4081 aacacagtat tgcaaggacg cggaacatgc ctcatgtggc ggccaggacg gccagccggg
     4141 atcgggatac tggtcgttac cagagccacc gacccgagca aacccttctc tatcagatcg
     4201 ttgacgagta ttacccggca ttcgctgcgc ttatggcaga gcagggaaag gaattgccgg
     4261 gctatgtgca acgggaattt gaagaatttc tccaatgcgg gcggctggag catggctttc
     4321 tacgggttcg ctgcgagtct tgccacgccg agcacctggt cgctttcagc tgtaagcgtc
     4381 gcggtttctg cccgagctgt ggggcgcggc ggatggccga aagtgccgcc ttgctggttg
     4441 atgaagtact gcctgaacaa cccatgcgtc agtgggtgtt gagcttcccg tttcagctgc
     4501 gtttcctgtt tggggtcgtt tgcgggaagg ggcggaatcc tacgctaagg ctttggccag
     4561 cgatattctc cggtgagatt gatgtgttcc caggggatag gagaagtcgc ttgatatcta
     4621 gtatgacgtc tgtcgcacct gcttgatcgc ggccgcgata gctagatcgc gttgctcctc
     4681 ttctccatcc gcgttccaag ctgcggaaag gcacccataa gcgtacgcct ggtcgagcag
     4741 gcgacgcgga tcgacgtcca gcgcacgaga gaatgcgtcc gccatctgtg caatgcgtct
     4801 aggatcgaga caaaggtcgt ctctgtcagc cggatcgtag aacatattgg cggcgccaaa
     4861 gcccacttca ccgaccagac cgacgggatc tatcaccagc cagccgcgac tggagaacat
     4921 gatgttttca tgatgcagat cgccatgtag cccacgcagt tccgaggcat tgctcatcat
     4981 ttgatcggct ataatcgccg cgtggacgta gtcagtttga caacctgcgt tttgatcatc
     5041 gcgcgcccgc tgaaacaaag ctgcaaagcg atcccggatc gggagaaggg cagaaggcag
     5101 gggttcctca gatgcggcat acagcttcgc cattagttcc gctgcaattt cggtcgcctg
     5161 gtagtcgccg tgctcggcaa cgatgtgaga gagcattcgc tccccggcat attcgagcaa
     5221 catcagattg ttctcacgac cgagcaaccg gactgctccc ctcccattgc gccataccag
     5281 atagtcggcc ccgcgcagtt catcagcaat gtcttctata ggtttcaatc ccttgacgat
     5341 tgcaggagtc ccgtctggca atgaaacttt ccaaacgagg ctggaaaagg tgtccgcaat
     5401 gagaacaggt tgcgaaacgt gccaatgagc aggaaaaaca ggcggcatga acatcaaccc
     5461 caagtcagag ggtccaatcg cagatagaag gcaaggcgtt cgcggtcggg ggcttcgatc
     5521 cccaatacat tgaataggac agcgaaggcg cgctctgctt catctggcgc tgcccagttc
     5581 tcttcggcgt tagcaatcat gagtgccaaa tcggcatagc gatctgctgt tccgagccgc
     5641 ccaaggtcga tcagacccgt gcattgaaga gttttagggt ccaccatgaa gttcggcatg
     5701 cagggatcac catggcaaac aaccatatcg gtgcgctctt ggtcgagccg caccggtagc
     5761 tctcgttcga cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc gtccggtaag
     5821 aagtcgggat tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa cattcgcgac
     5881 agcctgcgct caaacggaca ttgatcaacc gataggctgt gaacagcgcc aagttgctgc
     5941 cccattgacg gccacgcttt gagcaaatcc gctccagaca gatcagccgc cggtactccc
     6001 ggaattgccg ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt gatgacctcg
     6061 gggcaagcca cacctcgacc tttgagccaa atgaggcggt cacgctctcc agcgagctca
     6121 ccgcggcggg aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg tcgaaaaaca
     6181 aaatcaccag attctccgcc tctgacaggc aaccagtcag aatgcgattc acc
//
LOCUS       DAJTNW010000155         6228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNW010000155 DAJTNW010000000
VERSION     DAJTNW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            64..2496
                     /locus_tag="OO179_003804"
     CDS             64..2496
                     /locus_tag="OO179_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5533699.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="OO179_003805"
     CDS             2563..4032
                     /locus_tag="OO179_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5533700.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="OO179_003806"
     CDS             4032..5693
                     /locus_tag="OO179_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5533701.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..>6228
                     /gene="symE"
                     /locus_tag="OO179_003807"
     CDS             5914..>6228
                     /gene="symE"
                     /locus_tag="OO179_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5533702.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGV"
BASE COUNT         1842 a         1477 c         1452 g         1457 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggta
//
LOCUS       DAJTNW010000156         6214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNW010000156 DAJTNW010000000
VERSION     DAJTNW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            96..1484
                     /gene="narU"
                     /locus_tag="OO179_003808"
     CDS             96..1484
                     /gene="narU"
                     /locus_tag="OO179_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5533703.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINDGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1566..5306
                     /gene="narZ"
                     /locus_tag="OO179_003809"
                     /pseudo
     CDS             1566..5306
                     /gene="narZ"
                     /locus_tag="OO179_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5303..>6214
                     /gene="narH"
                     /locus_tag="OO179_003810"
     CDS             5303..>6214
                     /gene="narH"
                     /locus_tag="OO179_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5533704.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDLYRVGSLTAAACH"
BASE COUNT         1458 a         1656 c         1649 g         1451 t
ORIGIN      
        1 tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct
       61 tttttcatta ccaatgtgtg catatgagga acaatatggc actgcaaaat gagaaaaata
      121 gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa aataaaggaa
      181 aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg
      241 tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta
      301 ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc
      361 cctactcctt tatggtgcct atattcggtg gacgccgatg gccggttttt agtactgcaa
      421 tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg
      481 ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca
      541 tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg
      601 acggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg
      661 tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt
      721 cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcct
      781 ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct
      841 tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta
      901 tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc
      961 tgcgcctggc gttctttggc ccatttattg gtgccatcgc gcggtcggtt ggtggtgcta
     1021 tttccgataa attcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt
     1081 tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt
     1141 acgccgtatt tatggggctg tttctgaccg cgggtctggg aagtggttct actttccaga
     1201 tgatcgccgt catctttcgc cagataacca tttatcgggt gaagatgaaa ggcggtagtg
     1261 atgagcaagc tcagagagaa gccgtcaccg aaacggcggc aacgctgggc tttatctcag
     1321 ccattggcgc agtgggcggc ttttttattc cgcaggcatt tggcatgtcg ctcaatatga
     1381 ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc
     1441 tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat
     1501 gtacaaccat gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc
     1561 atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg
     1621 ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc
     1681 gttggcagtt cgacaaaatc gtgcgttcca cccacggcgt taactgtaca ggctcctgta
     1741 gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc
     1801 cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt
     1861 actcctggta tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga
     1921 ttgaactgtg gcgcgaagcc ctcaaacaac acagcgatcc ggtactggcg tgggcatcga
     1981 ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta
     2041 tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca
     2101 aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt
     2161 cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg cacctgttaa agtttctacg
     2221 actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac
     2281 cggaatctgc cgactggtat aactccagct acatcatcgc ttgggggtct aacgtaccgc
     2341 agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca
     2401 tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga
     2461 aacaaggcac tgatagcgcc ctggcgatgg caatggggca tgtgatttta aaagagtttc
     2521 atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga
     2581 tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg
     2641 catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag
     2701 ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa
     2761 aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc
     2821 tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa
     2881 atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa
     2941 acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg
     3001 cgaattacgg cctcgatcgc gggctggaag atgaaaacag cgcgaaagat tacgctgaaa
     3061 tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtaccgcgc cagtatattg
     3121 aaaccatcgc ccgcgaattt gccgataccg cccataaaac gcatgggcgc tcaatgatta
     3181 tcctcggcgc aggtgttaac cactggtatc acatggacat gaactaccgc gggatgatca
     3241 atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg
     3301 gccaggaaaa actgcgccca caaaccggct ggttgccact ggcctttgcg ctcgactgga
     3361 accgaccacc gcgccagatg aacagcacct cgtttttcta caatcattcc agccaatggc
     3421 gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca
     3481 gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgc
     3541 cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc
     3601 ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac
     3661 cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct
     3721 cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc
     3781 agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg
     3841 cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct
     3901 gcctgttctc cgatatcgtt ctgcccaccg ccacctggta tgaaaaagac gatatgaaca
     3961 cctcggatat gcatccattt attcatccgc tttctgcggc ggtcgatccg gcctgggaat
     4021 cacgcagcga ctgggaaatc tacaaaggta ttgccaaagc attttcgcaa gtgtgcgtgg
     4081 gccatcttgg caaagaaacc gacgtggtat tacaaccact gctgcatgac tctccggcag
     4141 agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgatcccgg
     4201 gtaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct
     4261 ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga
     4321 atacccagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg
     4381 cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg
     4441 caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg
     4501 ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata
     4561 ttcaggcgca gccgcgtaaa attatctcca gccccacatg gtccggtctg gaaagcgatc
     4621 acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt
     4681 cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg
     4741 tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag ataccgccaa
     4801 acggcttcca ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact
     4861 caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga
     4921 tcagcgaaac agatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtattta
     4981 acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga
     5041 ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca
     5101 tgcgcggcgg cattcataac tcggtcaccc gcgtttgccc gaaaccgacg catatgattg
     5161 gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcggc tcgaaccgcg
     5221 acgaattcat catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg
     5281 atcaggtaca ggaggcgaaa aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct
     5341 cgataaatgt atcggctgcc atacctgttc ggtgacctgt aacaacgtct ggaccggacg
     5401 cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc
     5461 gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa
     5521 gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt
     5581 ggtgccgcag cagattgatg attactacga acctttcacc ttcgactacg aacatttgca
     5641 tagcacaccg gaaggcaaac atatccctac tgctcgcccg cgttcactga ttgacggcaa
     5701 acggatggac aaagtgatct ggggcccaaa ctgggaagaa ctgctgggcg gcgagtttga
     5761 aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag gagatgtacg ggcagtttga
     5821 aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac tgcctcaatc ccagctgcgt
     5881 ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa gacggcattg tgctgattga
     5941 tcaggataaa tgccgtggct ggcgtttgtg cattagcggt tgtccgtaca aaaaaatcta
     6001 cttcaactgg aaaagcggca agtcagaaaa atgtatcttc tgttatccgc gaattgagtc
     6061 aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc atccggtatc tgggcgtgct
     6121 gctttacgac gccgaccgca tcgaggaagc gtcgagcacc gagcgcgaag ttgacctcta
     6181 tcgagtaggc agcctgacgg ctgcggcttg tcat
//
LOCUS       DAJTNW010000157         6212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNW010000157 DAJTNW010000000
VERSION     DAJTNW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.157"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..378
                     /locus_tag="OO179_003811"
     CDS             <1..378
                     /locus_tag="OO179_003811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533705.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO179_003812"
     CDS             complement(454..918)
                     /locus_tag="OO179_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5533706.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO179_003813"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO179_003813"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5533707.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO179_003814"
     CDS             complement(1787..2476)
                     /locus_tag="OO179_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5533708.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO179_003815"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO179_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5533709.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO179_003816"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO179_003816"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5533710.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO179_003817"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO179_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5533711.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1575 a         1688 c         1547 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg ac
//
LOCUS       DAJTNW010000158         6057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNW010000158 DAJTNW010000000
VERSION     DAJTNW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            381..1409
                     /gene="torT"
                     /locus_tag="OO179_003818"
     CDS             381..1409
                     /gene="torT"
                     /locus_tag="OO179_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5533712.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OO179_003819"
     CDS             complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OO179_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5533713.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2161..3333
                     /gene="torC"
                     /locus_tag="OO179_003820"
     CDS             2161..3333
                     /gene="torC"
                     /locus_tag="OO179_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5533714.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3333..5873
                     /gene="torA"
                     /locus_tag="OO179_003821"
     CDS             3333..5873
                     /gene="torA"
                     /locus_tag="OO179_003821"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR5533715.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5870..>6057
                     /gene="torD"
                     /locus_tag="OO179_003822"
     CDS             5870..>6057
                     /gene="torD"
                     /locus_tag="OO179_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5533716.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1679 g         1391 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgagat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcatta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
     1141 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     1201 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
     1261 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     1321 ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg gtctattttt
     1381 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
     1441 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     1501 ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt tcgcccggat
     1561 tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
     1621 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagtagt
     1681 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
     1741 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     1801 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     1861 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
     1921 gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca ccgctcgccg
     1981 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     2041 gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     2101 atctgttcat atttgctcat taagatcgct tcactaaacc ataattctac aggggttatt
     2161 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     2221 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     2281 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     2341 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     2401 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caacgacatt
     2461 taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     2521 gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgtcgctcc
     2581 tgccataact acgatgcgat ggatcatgcc aggcagcatc ctgaagcggc acgtcagatg
     2641 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     2701 ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat
     2761 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     2821 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     2881 gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc
     2941 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     3001 gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     3061 gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcctat
     3121 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     3181 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat
     3241 aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     3301 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     3361 cacgtcggcg ttttctggca caacttggcg gcttaaccgt cgccgggatg ctggggccgt
     3421 cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc gactgaggct gtcatctcga
     3481 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgatggtg aaggatggtc
     3541 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat
     3601 tgccggatca cgtacacaac gcggcacgta ttcgttatcc gatggtacgc gtggactggc
     3661 tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt gtgcgcgtga
     3721 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag aaaactcacg
     3781 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     3841 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     3901 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcaatg gaagtgtatg
     3961 aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct
     4021 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     4081 attacgagca gctaaaagcg aaagtcgccg ccggtgaagt tgaggtcatc agcatcgatc
     4141 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     4201 acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca tacgctgtac agtgaaaacc
     4261 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtatc
     4321 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     4381 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     4441 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     4501 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggc tggcactaca
     4561 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     4621 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcact attccgattg
     4681 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     4741 taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     4801 agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata
     4861 accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg
     4921 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     4981 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     5041 gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     5101 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     5161 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     5221 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     5281 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     5341 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     5401 tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg gaaacgctgc
     5461 gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg
     5521 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     5581 caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac gaaggggcat
     5641 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     5701 tgctgaccat cgacatcggg acttcgcagc tggcgcaggc ggccagtgcg cacactacgc
     5761 tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt aacggccccg
     5821 tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     5881 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     5941 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttcgttgct
     6001 gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg gaaaacagta ttgccac
//
LOCUS       DAJTNW010000159         6053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNW010000159 DAJTNW010000000
VERSION     DAJTNW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            431..1339
                     /locus_tag="OO179_003823"
     CDS             431..1339
                     /locus_tag="OO179_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5533717.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OO179_003824"
     CDS             complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OO179_003824"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5533718.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3654..4412
                     /gene="loiP"
                     /locus_tag="OO179_003825"
                     /gene_synonym="yggG"
     CDS             3654..4412
                     /gene="loiP"
                     /locus_tag="OO179_003825"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5533719.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OO179_003826"
     CDS             complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OO179_003826"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5533720.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5674..>6053)
                     /locus_tag="OO179_003827"
     CDS             complement(5674..>6053)
                     /locus_tag="OO179_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533721.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1490 a         1575 c         1563 g         1425 t
ORIGIN      
        1 gctgaatgta taactgagat tgatgattct ggaaaataat cactaagccg cataaattat
       61 ccttatttta tttttagggt accgtccggc ggtaaaaata aaatactcgt gcggtttaat
      121 gaaagcaatt gggtacgcga gaagaaaaac cccacaaacc aaaataataa aaccttaaat
      181 aacaacaata tgaaattgat atttttaaag tcaactttgt atttgttatg ttcatcacaa
      241 aatcaacatt ttgtttgtta ttgtatttgt gatgcatatc tctatttatt tattgaatgt
      301 tcgaaaacat acttttctaa tgcaatgttt tttcgctata cccctttata gattctgaat
      361 tctcacctgt cggctgtttt ctcctcatct catgtgacat attcccatac tttactcaag
      421 gaagattgtc atgaaagcac ctgtttcatt aacctcattt cgcccacaaa aatctcttgc
      481 tatcgccatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      541 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgaaccggg
      601 gctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      661 tgtggtctat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      721 atcagtcagc ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac
      781 tattgaagca ttaaaagatc gtctaattgt ccgtcagttg ccaactcagc tggagaacat
      841 tttcggccaa tataccgcca tcagcgccgt acaggatcgt acaaaattgg ttcaggatct
      901 gcaaaatgcc atgcgcaaag ccgttgttgg gccagtggta attgacgggg tccagattga
      961 aaatattgat ttttctgacg cttatgaaaa gtcgattgaa aaccgaatga aagcagaagt
     1021 cgccattgcc acgcgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcggt
     1081 aactcaggcc caggcagaag cagacagtaa actggcggca gccaaagtag aagcggaaac
     1141 tattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
     1201 agaagcaatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg tagccctcac
     1261 cactgccgaa cgttgggacg gtaaactgcc cgataccatg atccccggca gcacagttcc
     1321 gtttatctct actaagtaaa aaaagggccg ctttcgcggt ccttctcaca aaacgtctta
     1381 ctgattacag cagcgctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt
     1441 caaacagcag ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcgccgt
     1501 tcaggccaac atacttgtac cagtagtcag caatacccgc ttctaccgca acgcgtgcag
     1561 taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtca aatgcgtcgg
     1621 tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag
     1681 caaccgccag ttcaacttca gaaccggtgg cgatgaagat cagttccggc tgaccagcac
     1741 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct
     1801 gctgcgccag gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc
     1861 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg
     1921 gggtcacgcg cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca
     1981 gaccgatgga gtcgtgggtg taaaccatca cctggcgctg tttcatcagc gcagccatac
     2041 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     2101 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     2161 ggatgtagtt acctgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     2221 tagacggtgc caggtcagca gagccgccga ggaattcagg caacagcggg ccgaacgctt
     2281 cgatagcatt ctgcgacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
     2341 cgataaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     2401 taaattcagc cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg
     2461 cagattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     2521 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     2581 ccagcggcgc accgtgggag tcgtgagtac cggctttgtt cggggaaccg aaaccgatga
     2641 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tcaactgcgc
     2701 gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     2761 cgaaacgcat tgctgtgtcg tcggtgaacc agccttcaac gtgaccatcg atagaaatac
     2821 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     2881 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     2941 cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     3001 taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg
     3061 tataacctac ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     3121 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     3181 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     3241 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
     3301 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     3361 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     3421 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     3481 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     3541 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     3601 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     3661 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     3721 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     3781 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     3841 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     3901 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     3961 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acagataacg
     4021 aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat gtgaagaaag
     4081 ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
     4141 ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta gtcaattcgc
     4201 aattctccca gcgtcaggaa gcagaagccg atgattattc ttacgatctt ctgcaccaac
     4261 gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcgaag ctggaagaag
     4321 gccgtcaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgtgcc cagcatattc
     4381 gcgatcgcat cagcgcggat gggattaagt aaagcctagt ggtgtcgaac gtaggtcaga
     4441 taaggcgttc acgccgcatc tgacattaat ggcacatttg ttaccttgtg cgcattgccg
     4501 gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg
     4561 ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta
     4621 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
     4681 agcggtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
     4741 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat
     4801 cactggcgta ccggttcccg gcgcgaaagc aggatccagg cagtcgatat caaaagtcag
     4861 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctgcg
     4921 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg
     4981 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
     5041 catagtgccg tggtcaaatt cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg
     5101 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
     5161 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
     5221 ctgcagcttt tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc
     5281 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
     5341 cagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga
     5401 agtggccata tcgaacggca cgccagtaat cacccagtct gcatcgctgt catacggctg
     5461 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
     5521 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
     5581 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
     5641 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
     5701 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
     5761 gccttgtaat tcaaaggcgg caaagccgat taccccggta ttacgcacat cgccttgcgc
     5821 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cagtatgttt
     5881 atctttgcgg aatcctttaa tcactaacga atctccggca ttcacaatat agccgttgtt
     5941 attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcagccgtag caacgatctc
     6001 gtagttggtg ttacgactgt aattacggac atataattga taattcattc cga
//
LOCUS       DAJTNW010000160         6028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNW010000160 DAJTNW010000000
VERSION     DAJTNW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO179_003828"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO179_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5533722.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO179_003829"
     CDS             1207..1869
                     /locus_tag="OO179_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5533723.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO179_003830"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO179_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5533724.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO179_003831"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO179_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO179_003832"
     CDS             2795..3100
                     /locus_tag="OO179_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5533725.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO179_003833"
     CDS             3103..3507
                     /locus_tag="OO179_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5533726.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO179_003834"
     CDS             3497..3796
                     /locus_tag="OO179_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5533727.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO179_003835"
     CDS             3982..4278
                     /locus_tag="OO179_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5533728.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO179_003836"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO179_003836"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5533729.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO179_003837"
     CDS             5627..5995
                     /locus_tag="OO179_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5533730.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1525 a         1470 c         1518 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccacaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DAJTNW010000161         5961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTNW010000161 DAJTNW010000000
VERSION     DAJTNW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.161"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO179_003838"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO179_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5533731.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO179_003839"
     CDS             complement(1433..1759)
                     /locus_tag="OO179_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5533732.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO179_003840"
     CDS             1894..2235
                     /locus_tag="OO179_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5533733.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO179_003841"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO179_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO179_003842"
     CDS             complement(2833..3543)
                     /locus_tag="OO179_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5533734.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO179_003843"
     CDS             3651..3956
                     /locus_tag="OO179_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5533735.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OO179_003844"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OO179_003844"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="OO179_003845"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="OO179_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1606 a         1502 c         1399 g         1454 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca t
//
LOCUS       DAJTNW010000162         5931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTNW010000162 DAJTNW010000000
VERSION     DAJTNW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1250
                     /locus_tag="OO179_003846"
     CDS             <1..1250
                     /locus_tag="OO179_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HCR5533736.1"
                     /translation="DYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTD
                     NADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLANTLT
                     IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQAS
                     ALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSS
                     KKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKE
                     IKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVA
                     LAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRP
                     ASEQPPAQPRLRITEQDPNWETF"
     gene            1296..2897
                     /gene="tap"
                     /locus_tag="OO179_003847"
     CDS             1296..2897
                     /gene="tap"
                     /locus_tag="OO179_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5533737.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            2916..3776
                     /gene="cheR"
                     /locus_tag="OO179_003848"
     CDS             2916..3776
                     /gene="cheR"
                     /locus_tag="OO179_003848"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5533738.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            3779..4828
                     /gene="cheB"
                     /locus_tag="OO179_003849"
     CDS             3779..4828
                     /gene="cheB"
                     /locus_tag="OO179_003849"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5533739.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            4843..5232
                     /gene="cheY"
                     /locus_tag="OO179_003850"
     CDS             4843..5232
                     /gene="cheY"
                     /locus_tag="OO179_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5533740.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            5243..5887
                     /gene="cheZ"
                     /locus_tag="OO179_003851"
     CDS             5243..5887
                     /gene="cheZ"
                     /locus_tag="OO179_003851"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5533741.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
BASE COUNT         1433 a         1455 c         1734 g         1309 t
ORIGIN      
        1 ttgattatct tgattatggc aatactggag cttatttcgc tcagccaacc cagggaatgc
       61 aaaatgcaat gggcgaagcg tttgctcagt acgccctcag cagtgaaaaa ctgtatcgcg
      121 atatcgtcac tgacaacgca gatgattacc gatttgccca gtggcaactg gcggttatcg
      181 cgctggtggt ggtattgatt ctgctggtgg cgtggtacgg cattcgccgt atgttgctta
      241 ctccgctggc aaaaattatt gctcacattt gcgaaatcgc cggtggtaac ctggcgaata
      301 ccctgaccat tgacgggcgc agtgaaatgg gtgacctggc gcagagcgtt tcacatatgc
      361 aacgctcttt gactgacacc gtcactcatg tccgcgaagg ttcagatgcc atctatgccg
      421 gtacccgtga aattgcggcg ggcaacaccg atctttcctc ccgtactgaa cagcaggcat
      481 ccgcgctgga agaaactgcc gccagcatgg agcagctcac cgcgacagtg aagcaaaacg
      541 ccgataacgc ccgccaggcc tcgcaactgg cgcaaagtgc ctccgacacc gcccagcacg
      601 gcggcaaagt ggtggatggc gtagtgaaaa cgatgcatga gatcgccgat agttcgaaga
      661 aaattgccga cattatcagc gttatcgacg gtattgcctt ccagactaat atcctcgcgc
      721 tgaatgccgc ggttgaagcc gcgcgtgcgg gtgaacaggg ccgtggtttt gccgtggtgg
      781 cgggtgaagt gcgtaatctt gccagtcgca gcgcccaggc ggcaaaagag atcaaagccc
      841 tcattgaaga ctccgtctcg cgcattgata ccggttcggt gctggtcgaa agcgccgggg
      901 aaacaatgaa caatatcgtc aatgctgtca ctcgcgtgac tgacattatg ggcgagattg
      961 catcggcatc ggatgaacag agccgtggca tcgatcaagt cgcattggcg gtttcggaaa
     1021 tggatcgcgt cacgcaacag aacgcatcgc tggtgcagga atcagctgcc gccgcagctg
     1081 cgctggaaga acaggcgagt cgtttaacgc aagcggtttc cgcgttccgt ctggcagcca
     1141 gcccactcac caataaaccg caaacaccat cccgtcctgc cagtgagcaa ccaccggcac
     1201 agccacgact gcgaattact gaacaagatc caaactggga aacattttga cgagttttta
     1261 ctaacgcggt cattgccgcc tgatggggag cgttgatgtt taatcgtatt cgaatttcga
     1321 ccacgctgtt tttaattttg attctctgcg ggatcttgca gattggcagt aacggcatgt
     1381 ctttttgggc atttcgcgac gatttgcaac gactgaatca cggcgagcag agcaatcagc
     1441 aacgtgcggc attagcgcaa actcgggcgg taatgttaca ggccagtacc gcgctgaaca
     1501 aagcaggcac tctgacagcg cttagctatc cggcggatga cattaaaacg ttgatgacga
     1561 cggcgcgcgc cagtctgacg caatccacca cgctgtttaa aagttttatg gcgatgactg
     1621 cgggcaacga gcacgtcagg gcattgcaaa aagagacgga gaaaagtttt gcccgctggc
     1681 acaacgatct cgaacatcag gcgacctggc ttgaaagtaa tcaactttcg gatttcctca
     1741 ctgcgccggt gcagggatca cagaatgcgt ttgacgttaa ctttgaggcc tggcagctgg
     1801 agatcaacca tgtgctggaa gccgccagtg cgcaaagcca gcgtaactat cagatttcgg
     1861 cgctggtgtt tatcagcatg attattgttg cggcgatcta catcagcagt gcgctgtggt
     1921 ggacgcgcaa gatgattgtt caaccactgg ccattatcgg tagccatttt gacagcattg
     1981 ctgcgggtaa tctggcgcgt ccgattgcgg tatatggtcg taatgagatc accgccattt
     2041 ttgccagcct gaagaccatg cagcaggctt tgcgtgggac ggtgagtgat gtgcgtaagg
     2101 gaagccatga gatgcacatt gggatcgcgg agattgtcgc aggcaataac gatctctcaa
     2161 gtcgtaccga acagcaggcg gcatcgctgg cacaaacggc cgccagtatg gagcaattaa
     2221 ccgccacggt agggcaaaac gccgataacg cacgacaggc gtcggaactg gcaaaaaatg
     2281 ccgcgacaac ggcgcaggcg ggcggtgttc aggtcagtac catgactcac accatgcagg
     2341 agatcgccac cagctcgcaa aaaattggcg acattatcag cgttatcgac ggaattgctt
     2401 tccagaccaa tattctggcc ctgaatgcgg cagtggaagc ggctcgcgcc ggagagcagg
     2461 ggcgtggttt tgcggtagtg gcaggtgaag tgcgcaatct tgccagccgt agcgcgcagg
     2521 cagcaaaaga gatcaaaggg ctgatcgaag agtcagtcaa tcgtgtccag cagggttcga
     2581 aactggtgaa taacgccgcc gcgaccatga ccgatattgt cagttcggtg acccgcgtga
     2641 acgacattat gggagaaatt gcttcggcgt cggaagaaca acggcggggg attgagcagg
     2701 ttgcacaggc tgtcagccag atggatcagg tgactcagca gaactcctcg ctggtagaag
     2761 aagcggcggt ggcaacggaa caactggcga atcaggccga ccatctttcg tcgcgtgtgg
     2821 cggtatttac ccttgaagaa catgaagtag cacgacatga gtcggcgcag ttacaaattg
     2881 cgccagtggt atcctgaagt gattgagaag gcgctatgac ttcatctctg ccctgtgggc
     2941 aaacgtcttt attgttacag atgaccgagc gcctggcgct ttccgacgcg cattttcggc
     3001 ggataagtca attgatctat caacgagccg ggatcgttct ggctgaccat aaacgcgaca
     3061 tggtttacaa ccgactggtt cgtcgtttgc gttcgctggg actgacggat ttcggtcatt
     3121 atctgaactt gctggaatct aatcagcaca gcggtgagtg gcaggcgttt atcaattcgc
     3181 tgaccacgaa tctgacggca tttttccgtg aggctcatca tttccctctg ctcgcggatc
     3241 acgcacgtcg ccgttctggc gaggatcgcg tatggagcgc ggcggcttcg accggcgaag
     3301 agccgtacag cattgcgatg actctggctg acacattggg caccgtgccc ggacgctgga
     3361 aagtgtttgc cagtgatatc gacaccgaag tgctggaaaa agccagaagc ggtatctatc
     3421 gccatgaaga gttgaaaaac ctgacgccgc agcaactgca acggtatttc atgcgaggga
     3481 cgggaccgca tgaagggctg gtacgcgtgc gtcaggagct ggcgaactat gttgattttg
     3541 ccccgctgaa tttactggcg aaacagtaca ccgtgccggg gccgtttgat gcgatcttct
     3601 gtcgtaacgt catgatctac ttcgatcaaa ctacccagca ggagattttg cgccgctttg
     3661 ttccgctcct taaacccgac ggattgctgt ttgcgggtca ctctgaaaac tttagccacc
     3721 tcgagcgccg cttcacgctg cgtggtcaga cggtgtatgc gctaagtaag gattaacgat
     3781 gagcaaaatc agggtgttat ctgtcgatga ttcggcactg atgcgccaga tcatgacaga
     3841 aatcatcaac agccatagcg acatggaaat ggtggcgacc gcgcctgatc cgctggttgc
     3901 gcgtgacttg attaagaaat tcaatcccga tgtgctgacg ctggatgttg aaatgccgcg
     3961 gatggacgga ctggatttcc tcgaaaaatt aatgcgtttg cgtccaatgc ccgttgtgat
     4021 ggtttcttcc ttgaccggca aaggatcaga agtcacgctg cgcgcgctgg agctgggggc
     4081 aatagatttt gtcaccaaac cgcaactggg tattcgcgaa ggaatgctgg cgtatagcga
     4141 aatgattgct gaaaaggtgc gtacggcagc aaaggcgagc cttgcagcac ataagccatt
     4201 gtcagcaccg acaacgctga aggcagggcc gttgttgagt tctgaaaaac tgattgcgat
     4261 tggtgcttca acgggtggaa ctgaggcaat tcgtcacgta ctgcaaccgt tgccgctttc
     4321 cagcccggca ttgttaatta cccaacatat gccgcccggt ttcacccgct cttttgccga
     4381 cagacttaat aagctttgcc agatcggggt taaagaagcc gaagacggag aacgtgtcct
     4441 gccggggcat gcctatattg cgccgggcga tcggcatatg gaactggcgc gtagtggcgc
     4501 aaattatcaa atcaaaattc acgacggccc ggcggttaac cgtcatcggc cttcggtaga
     4561 tgtgttgttc cattctgtcg ccaaacaggc ggggcgtaat gcggttgggg tgatcctgac
     4621 cggtatgggc aacgacggcg cggcgggaat gttggcgatg cgtcaggcgg gggcatggac
     4681 ccttgcgcaa aacgaagcaa gttgcgtggt gttcggcatg ccgcgcgagg ccatcaatat
     4741 gggtggtgtc tgcgaagtgg tcgatcttag ccaggtaagc cagcaaatgt tggcaacaat
     4801 tagtgccgga caggcgatac gtatttaaat caggagtgtg aaatggcgga taaagaactt
     4861 aaatttttgg ttgtggatga cttttccacc atgcgacgca tagtgcgtaa cctgctgaaa
     4921 gagctgggat tcaataatgt tgaggaagcg gaagatggtg tcgacgctct caataagttg
     4981 caggcaggcg gttatggatt tgttatctcc gactggaaca tgcccaatat ggatggcctg
     5041 gaattgctga aaacaattcg tgcggatggc gcgatgtcgg cattgccagt gttaatggtg
     5101 actgcagaag cgaagaaaga gaacatcatt gctgcggcgc aagcgggggc cagtggctat
     5161 gtggtgaagc catttaccgc cgcgacgctg gaggaaaaac tcaacaaaat ctttgagaaa
     5221 ctgggcatgt gaggatgcga ctatgatgca accatcaatc aaacctgctg acgagcattc
     5281 agctggcgat atcattgcgc gcatcggcag cctgacgcat atgctgcgcg acagtttgcg
     5341 ggaactgggg ctggatcagg ccattgccga agcggcggaa gccatccccg atgcgcgcga
     5401 tcgtttgtac tatgttgtgc agatgaccgc ccaggctgcg gagcgggcgc tgaacagtgt
     5461 tgaggcgtca caaccgcatc aggatcaaat ggagaaatca gcaaaagcgt taacccaacg
     5521 ttgggatgac tggtttgccg atccgattga ccttgccgac gcccgtgaac tggtaacaga
     5581 tacacgacaa tttctggcag atgtacccgc gcataccagc tttactaacg cgcaactgct
     5641 ggaaatcatg atggcgcagg attttcagga tctcaccggg caggttatta agcggatgat
     5701 ggatgtcatt caggagatcg aacgccagtt gctgatggtg ctgttggaaa acatcccgga
     5761 acaggagtcg cgtccaaaac gtgaaaacca gagtttgctt aatggacctc aggtcgatac
     5821 cagcaaagcc ggcgtggtag ccagtcagga tcaggtggac gatttgttgg atagtcttgg
     5881 attttgattt gtattgcctg aacttcggcc tgcgcgatga tgccaagccg a
//
LOCUS       DAJTNW010000163         5812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTNW010000163 DAJTNW010000000
VERSION     DAJTNW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..205)
                     /locus_tag="OO179_003852"
     CDS             complement(<1..205)
                     /locus_tag="OO179_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533742.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSK"
     gene            complement(615..986)
                     /locus_tag="OO179_003853"
     CDS             complement(615..986)
                     /locus_tag="OO179_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533743.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(1047..1544)
                     /locus_tag="OO179_003854"
     CDS             complement(1047..1544)
                     /locus_tag="OO179_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533744.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1620..2408)
                     /gene="aadA1"
                     /locus_tag="OO179_003855"
     CDS             complement(1620..2408)
                     /gene="aadA1"
                     /locus_tag="OO179_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5533745.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2466..2990)
                     /gene="sat2"
                     /locus_tag="OO179_003856"
     CDS             complement(2466..2990)
                     /gene="sat2"
                     /locus_tag="OO179_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5533746.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(3085..3558)
                     /locus_tag="OO179_003857"
     CDS             complement(3085..3558)
                     /locus_tag="OO179_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5533747.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3890..4426
                     /locus_tag="OO179_003858"
     CDS             3890..4426
                     /locus_tag="OO179_003858"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5533748.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4541..4867
                     /locus_tag="OO179_003859"
     CDS             4541..4867
                     /locus_tag="OO179_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533749.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(5055..5294)
                     /locus_tag="OO179_003860"
     CDS             complement(5055..5294)
                     /locus_tag="OO179_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5533750.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1516 a         1391 c         1296 g         1609 t
ORIGIN      
        1 tcttggatga cagcgtgtca ggaaatccaa tatcgaagtc ccgccccgtg tcaaagtagg
       61 gcatttcatg atcaaaggac gaagcttccc aggccacttc cctaatattc cagccgctag
      121 aaacaagatt tcgtacgact tcatcaactt ttttgctttc caacttgtca caaagtgcat
      181 aggcaagagc tttggcggca gacacgtgcg tttcctctta aacataggtc gaacggatac
      241 ccgaggcaaa gcggagcatt agtcccaaag catctccggt agatccggag gctccatact
      301 gaaggcatgg catcttgggc attgatattt gaaacttctc tctagatagc agggatttct
      361 tggccttgcc tcatgctgat agcttgtaaa tatcacacga tccttagccc cacaaagtga
      421 gcatactccg tagctttcat ttatagaaat cgtcgttaga ttgtcgcagc atgtgcacgt
      481 tccgactatt tgctcaggaa gcatcgccat accttggcct aggtaaagaa gatcagactc
      541 gtagccacat cggtggcaac tgagcttgta cgcaatgccc atctggttgt ctcacctgaa
      601 tcgtaacgtc agagctattc atccatccat tcggcatcaa tgtatgccac taagccgacc
      661 agctgaccgt ttggactgaa gtatgttgcc tgcaccccac ttgacatctc atcaatacca
      721 ccgcactgcg ctaggcgccg gtcccacttg cccacagaag cgtcaggatc ggctgagttc
      781 cccaagaccc ttgtgagcca tgcctccttt aactcaacct ctcgaatgat tgcgtcggcc
      841 agctcctccg cgttatctag ttcgacatag tctggaattc ggacgtacgt tctgaacaca
      901 gctggatact tacttgggcg attgtcatac atgacatcaa caatgtaccc gtttgtgtaa
      961 ccgtctcttg gaggttcgta tgacactagt ggttcccctc agcttgcgac tagatgttga
     1021 ggcctaacat tttattagag agcaggctag ttgcttagat acatgatctt caggccgtta
     1081 tctgtcaggg caagcgaaaa ttggccattt atgacgacca atgccccgca gaagctccca
     1141 tctttgccgc catagacgcc gcgcccccct tttggggtgt agaacatcct tttgccagat
     1201 gtggaaaaga agttcgttgt cccattgttg gcaatgacgt agtagccggc gaaagtgcga
     1261 gacccatttg cgctatatat aagcctacga tttccgttgc gactattgtc gtaattggat
     1321 gaactattat cgtagttgct ctcagagttg tcgtaatttg atggactatt gtcgtaattg
     1381 cttatggagt tgtcgtagtt gcttggagaa atgtcgtagt tggatgggga gtagtcatag
     1441 ggaagacgag cttcatccac taaaacaatt ggcaggtcag caagtgcctg ccccgatgcc
     1501 atcgcaagta cgaggcttag aaccaccttc aacagatcgc gcatagtctt ccccagctct
     1561 ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat
     1621 tatttgccga ctaccttggt gatctcgcct ttcacgtagt gaacaaattc ttccaactga
     1681 tctgcgcgcg aggccaagcg atcttcttgt ccaagataag cctgcctagc ttcaagtatg
     1741 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1801 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1861 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1921 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1981 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     2041 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     2101 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     2161 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     2221 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     2281 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     2341 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     2401 tccctcatga tgtttaactc ctgaattaag ccgcgccgcg aagcggtgtc ggcttgaatg
     2461 aattgttagg cgtcatcctg tgctcccgag aaccagtacc agtacatcgc tgtttcgttc
     2521 gagacttgag gtctagtttt atacgtgaac aggtcaatgc cgccgagagt aaagccacat
     2581 tttgcgtaca aattgcaggc aggtacattg ttcgtttgtg tctctaatcg tatgccaagg
     2641 agctgtctgc ttagtgccca ctttttcgca aattcgatga gactgtgcgc gactcctttg
     2701 cctcggtgcg tgtgcgacac aacaatgtgt tcgatagagg ctagatcgtt ccatgttgag
     2761 ttgagttcaa tcttcccgac aagctcttgg tcgatgaatg cgccatagca agcagagtct
     2821 tcatcagagt catcatccga gatgtaatcc ttccggtagg ggctcacact tctggtagat
     2881 agttcaaagc cttggtcgga taggtgcaca tcgaacactt cacgaacaat gaaatggttc
     2941 tcagcatcca atgtttccgc cacctgctca gggatcaccg aaatcttcat atgacgccta
     3001 acgcctggca cagcggatcg caaacctggc gcggcttttg gtacaaaagg cgtgacaggt
     3061 ttgcgaatcc gttgctgcca cttgttaacc cttttgccag atttggtaac tataatttat
     3121 gttagaggcg aagtcttggg taaaaactgg cctaaaattg ctggggattt caggaaagta
     3181 aacatcacct tccggctcga tgtctattgt agatatatgt agtgtatcta cttgatcgat
     3241 caggcttttg tatatctccc caccacctga aacaatgaca tgatccgtta ttttctttag
     3301 gttggttaaa gcatctttaa ttgatggaaa gatcaatacg ttctcattgt cagatgtaaa
     3361 acttgaacgt gttacgaccg catactttcg gttgggtaat gctcccattg attcaaaagt
     3421 cttgcgtcca accaacagcc attggttata ggtaatagct ttaaacagga gctgttcacc
     3481 tttggcactc catggaatat cagggccatt cccgataact ccattcttcg atatagctac
     3541 cattagtgat agtttcacaa ttcttcctca gaggttaaca ttttattagt aagcatgctc
     3601 gttttgtcac ccgctgatgc ttaccgttaa ttaatataat cacttgtatt aaatagactt
     3661 tctttaaaaa tacaagacac tctgttatta caaatcgtgc atgccgtcta tcctgcttgc
     3721 acgatgcaca ttatctcacc caaagaactc ataacatttt aatttattta gcattaaatt
     3781 aaaactctac cggtaaacaa gcatctctag gcgtaaaaaa caggcacttt tttaaatccc
     3841 agctagaata ggctgtatag gcagacagtt gcaagacaag gagcgtttta tgtctaacag
     3901 tccattttta aattctatac gcacggatat gcgacaaaaa ggttatgcgc tgaaaactga
     3961 aaaaacttac ctgcactgga ttaagcgttt tattctgttt cacaaaaaac gtcatcctca
     4021 gaccatgggc agtgaagagg tcaggctgtt tttatccagc ttagcaaaca gcagacatgt
     4081 agccataaac acgcagaaaa tcgctttaaa tgccctagct tttttgtaca acaggttttt
     4141 acaacagccg ttgggcgata ttgattatat ccctgcaagc aagcctagac ggctaccctc
     4201 tgttatctct gcaaatgaag tgcaacgcat tttgcaggtt atggatactc gcaaccaagt
     4261 tatttttacg ctgctgtatg gtgcaggttt gcgcattaat gaatgcttgc gtttgcgggt
     4321 taaagatttt gattttgata atggctgcat cactgtgcat gacggtaagg gtgggaaaag
     4381 cagaaacagc ctactgccca cgcgcctaat cccagcaata aaataactca ttgagcaagc
     4441 gcggcttatt cagcaagacg acaacttaca aggcgtaggg ccatcgctgc cttttgcttt
     4501 agatcacaaa tacccttctg cttatcgaca agcggcgtgg atgtttgtct ttccctccag
     4561 cacgctctgc aaccacccgt ataacggcaa attatgccgc catcatctgc atgactccgt
     4621 tgcgcgaaag gcattgaagg cagccgtaca aaaagcaggc atcgttagca agcgtgtcac
     4681 ttgtcataca tttcgtcact cgtttgctac gcatctatta caagcggggc gtgatattcg
     4741 cactgtgcaa gaactcttag ggcataacga tgttaagacc acgcaaatct atacgcatgt
     4801 gttgggtcag cattttgccg gcaccaccag tcctgcggat ggactgatgc tacttatcaa
     4861 tcagtaaaag tagataaata tctttatgta gccctgagta gcccctgata ctttttatgg
     4921 tgaaaaagat aaacaatcat agaggggata aatatgagcc agttctacca ttgttcagat
     4981 aagtttaagc aagatactgt caatcacagt gaccattcat ggtcattaca cttcctccag
     5041 ttgccagaca atgcctaaca taaaaacgca aaagcccaaa agtcagagct taggtgttct
     5101 aggcgttttc taagcgctga agaaatacgt cttaccgtag attgcgtgcg tttctgaagc
     5161 acattttcct tatacatggc tttatcccag gcagcttcat caagctcttg gagtaacaag
     5221 ccagtaataa tgcgacactc tcgggtttgt agcgagccgc tggtgagatc ggagttatag
     5281 cggaaagggg tcatttgtaa ttatttgtgg gtggaggata gttaacttat tattgtagtt
     5341 ggaagaggct gctttccaaa cattagcacc tcgtaatgct tgttactgta tattaattta
     5401 tgctcttagc gcatgttaat gggctattac aatgcataat gtccacatca agataactgt
     5461 ggcttaacac ctgtacaagc gaaaaaaatc aatcacctgt ccggaaatac gggatcactg
     5521 taaagtgttg gtgaggcagt ctaggagggg gatttaattt gggatactga tgaaaggatt
     5581 atgatcagtt ggatatagga tcgtactaag ctgagcggca ggttagctag attagttaac
     5641 cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac
     5701 gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc
     5761 catttccacc cctcccagtt cccaactatt ttgtccgccc acagggctgc cg
//
LOCUS       DAJTNW010000164         5807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNW010000164 DAJTNW010000000
VERSION     DAJTNW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..467)
                     /locus_tag="OO179_003861"
     CDS             complement(<1..467)
                     /locus_tag="OO179_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533751.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGT"
     gene            complement(469..747)
                     /locus_tag="OO179_003862"
     CDS             complement(469..747)
                     /locus_tag="OO179_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533752.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            688..873
                     /locus_tag="OO179_003863"
     CDS             688..873
                     /locus_tag="OO179_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533753.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(915..1070)
                     /gene="hokD"
                     /locus_tag="OO179_003864"
     CDS             complement(915..1070)
                     /gene="hokD"
                     /locus_tag="OO179_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5533754.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1722..2138)
                     /locus_tag="OO179_003865"
     CDS             complement(1722..2138)
                     /locus_tag="OO179_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533755.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            2165..2305
                     /locus_tag="OO179_003866"
                     /pseudo
     CDS             2165..2305
                     /locus_tag="OO179_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            2305..3345
                     /locus_tag="OO179_003867"
     CDS             2305..3345
                     /locus_tag="OO179_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533756.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(3373..3462)
                     /locus_tag="OO179_003868"
     tRNA            complement(3373..3462)
                     /locus_tag="OO179_003868"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3426..3428),aa:Ser,seq:cga)
     gene            3556..4353
                     /gene="mtfA"
                     /locus_tag="OO179_003869"
     CDS             3556..4353
                     /gene="mtfA"
                     /locus_tag="OO179_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5533757.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4454..4529
                     /locus_tag="OO179_003870"
     tRNA            4454..4529
                     /locus_tag="OO179_003870"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Asn,seq:gtt)
     gene            4691..5807
                     /locus_tag="OO179_003871"
                     /pseudo
     CDS             4691..5807
                     /locus_tag="OO179_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1542 a         1394 c         1372 g         1499 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaacaggt cgaagtaata cccgcacatt acctccagat
      481 ccgttgctgg aatgtgcagg acggacgcgg tggccgttcg gaataaggga gcctgacgaa
      541 gattatccag tgacgataat cgagactgag ggctttctta atctcgtatc cgtgtctgcg
      601 gtagcgctga atcagccatt cagcctgttc ttcggtgcag gggtcgtgct gatacccgtc
      661 agatttgaat gcatgagaac gccgcccgtg cctgctggca ggggcggcag agttatccga
      721 attgtaaaat ttggtatcgt gcgccatctg ttttctctgc tggcgcagca ggtgccagtt
      781 gttcaggctg acggatggat tgtaaaccag aacgaccaga aaaaacaaaa cccgccgaag
      841 cgggttaagt gcgggtgcgt tgaggatgcc tgacacatca gcggtggcga gggatttctc
      901 ccccgccggg tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg
      961 ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg
     1021 gttaaacaga tgacgataag ggcgattaac atcgcctttt gctgcttcat agcctgcttc
     1081 tccttgcctt tcggcacgta agaggctaac ctacatgtgt tcagcatgga ttgagcctca
     1141 gattaatgtt aagcgtcttg caggacgcgt aatgttaact ggggcttttc tctatctgcc
     1201 ttttggtgtt catgcctgag acagatagcc tcaagcaccc gcagtcattc tacttaacta
     1261 agatttccct gcaaaccgtt tttgtccggc acagtaaata tccaactaaa ccaatagcgt
     1321 tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc ctaaaaaaag
     1381 ggcatttata tgtccaaaca ttaatatcaa aacatcaatt ttttccatat accttgctgt
     1441 gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa actgcaatgc
     1501 gtttttcatt attcccccta caatcaatgt gcaataacat ttaaacacac ctcaatttgg
     1561 ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca aactctttag
     1621 tctaaatact catcgtaaaa cattccccat acttatcagt ccgttccgcg ccaggtagct
     1681 tattgcctta tctggcaacc tataatcagg tttccgcttt ttcagttggc tggtcgttta
     1741 accgacatag ttaacccatt aatctggttg ccggatgctg gtggattttc gcgttttaat
     1801 tgttcataaa agtgcacagc tttaaccagt tcttctgatg taaccgggac tggtggggca
     1861 gtgaataagg cctgaatttc atagttcggc ctgtcgttac aatcctcttt tttcggtaca
     1921 tatttccagt caccaaccca caactccccc tgagagtcca taacaccttt tttcacgtag
     1981 cgatatcgcc acgctatcgg ctctgcttcc agcgatgccc gtgcaatttt gaataactcg
     2041 ccctctactc gcgccatccc tgaattgggg tggcatttcg taatcgctat ttttaatttg
     2101 gcttcttcga ttaattgttc ttttgttaat tcagtcattt tcattaccgc cctggtaatg
     2161 ctgccaccag cgaaaatggt taatggaaaa cgggcttccg ttctatacca atcgtagtgg
     2221 caaaccgatt gtcagccggg atctatttac ctgcaataaa actttaccac cacgcgaggt
     2281 agagccgaat tttggtgcga tctgatggga agacgaagga aaaatcctga acacgaaaaa
     2341 ttacctccaa atgtataccc aaataaatat agttatgtat ggaaaccaac atccagagaa
     2401 tctgtaacac taaccgccat caaggatggt ttagctgctt tatggaaaaa gtatgaggaa
     2461 actgtaaata atcgcgatcg tgcaatgaca ttcggtcgct tgtgggaaaa attcctcgcc
     2521 agcgcctatt acagtgacct cagtccaaga acacaaaaag attatctgca acatcaaaaa
     2581 aagttgcttg ccgtattcgg taaggtgccg gcagattcca taaaaccaga acacatccgt
     2641 cgatacatgg acaagagagg ggaacagagt aaaacgcaag ccaaccatga aaaaagcagt
     2701 atgtcccgcg tttacagttg ggggtatgag cgagggtacg tgaaggctaa cccatgtgca
     2761 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
     2821 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
     2881 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
     2941 tttatccagc aagggaaaac aggaaaaaaa cagataaaag catggagtcc acgattacag
     3001 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     3061 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcgcgca
     3121 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     3181 tcagactatg aaggaagcag tcgggataag caacttttct ctggtcacaa aaccgagggg
     3241 caagtgctaa tctatgacag gaaggttaaa gtttcaccga cacttgatgt cccgttacct
     3301 gaaaatattc caagaaaata ttccaagtgt gatttttgtc actgacttaa tgatgtataa
     3361 gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg cccccactcc
     3421 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgtcatg
     3481 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     3541 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     3601 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac agaacaggaa
     3661 caaagcaaat tggtcgctct tgccgaacgt tttttacagc aaaaacggct tgttccttta
     3721 cagggctttg agctgaattc attaagaagc tgccggatag cacttctatt ttgcctgccc
     3781 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     3841 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     3901 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     3961 gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca taagctggac
     4021 acccgtaacg gcgatcgcgc cagcggagtt ccctttattt cgttgcgtga ggttgctggc
     4081 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attagttggt
     4141 gagaatgcgg cgagcattga tgcttatgtt gccagtgatc ctgctgaatg ttttgccgta
     4201 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     4261 caacgtttct gtcaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     4321 acagactcgt tttcagcgac gaatgttcat taattaacaa ctttgaagat taattaacca
     4381 attgaaatgg cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgcgcc
     4441 ccgttctcac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     4501 cactggttcg agtccagtca gaggagccaa ttttctgttt tcatgcatcc ttgcgaatct
     4561 ttatctgatg ttgattcaac aggttagtgt gaaaactctt ctcggtgcat tttgatttta
     4621 ccctacgcat cgggaaaaat tggtggtcaa atctgggatc aggttagttc gataatggag
     4681 tgacccccat atatccctta ccgacgcaaa aatccgcacc ctcaagcctt ctgataaacc
     4741 ctttaaagtc tccgattctc acggtctgta tctgctggtc aagccgggtg gctcccgcca
     4801 ctggtatctc aaataccgta ttagcggtaa agaatcccgc attgcgctgg gtgcctatcc
     4861 agccatctcc ctgtctgatg cgcgacagca acgtgaaggt atccgtaaaa tgctggcgct
     4921 gaatatcaac ccggtacagc agcgggctgc tgaacgtggc tcacgaacac cggagaaagt
     4981 ttttaaaaac atggcgctgg cgtgacataa aagtaacagg aaatggtcgc agaacaccgc
     5041 cgaccgtctg cttgccagcc tgaacaatca catctttccg gtcatcggga acctacctgt
     5101 atcagaactt aaaccccgtc atttcattga cctgctgaaa gggatcgagg aaaaaggtct
     5161 gctggaggtt gcgtcccgca cacggcagca cctgagtaac ataatgcgcc atgcggtcca
     5221 tcaggagtta atcgatacga accctgcagc aaaccttggc ggcgtgacca cacctcctgt
     5281 cagacggcac tatcctgccc tgccgctgga gcggctgcct gaactgcttg aacgtattgg
     5341 ggcatatcat cagggccgtg aactgacccg gcatgccgtt ctgctgatgc tgcatgtgtt
     5401 cattcgctcc agtgaactgc gtttcgcccg ctggtcagag attgatttca caaaccgagt
     5461 ctggacgata cccgcgacgc gagaacccat tattggcgtg cgttattccg gccgcggggc
     5521 aaaaatgcga atgccgcata tcgtccccct ctcagaacag tccatcgcca ttctgaaaca
     5581 gattaaggat atcaccggta ataatgaact gatcttcccc ggcgaccata acccgtataa
     5641 gccaatgtgt gaaaacacgg tcaataaggc actgcgggtg atgggttacg acacgaaaaa
     5701 ggatatctgc ggtcacggct tccgggcaat ggcatgcagt gcgctgatgg aatcgggttt
     5761 atgggcaaag gacgcagtag aacgccagat gagtcatcag gagcgca
//
LOCUS       DAJTNW010000165         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNW010000165 DAJTNW010000000
VERSION     DAJTNW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..219)
                     /locus_tag="OO179_003872"
     CDS             complement(<1..219)
                     /locus_tag="OO179_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5533758.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="OO179_003873"
     CDS             complement(216..1124)
                     /locus_tag="OO179_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5533759.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="OO179_003874"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="OO179_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO179_003875"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO179_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO179_003876"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO179_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5533760.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="OO179_003877"
     CDS             5533..>5702
                     /locus_tag="OO179_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533761.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAJTNW010000166         5692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNW010000166 DAJTNW010000000
VERSION     DAJTNW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="OO179_003878"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="OO179_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5533762.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="OO179_003879"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="OO179_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5533763.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="OO179_003880"
     CDS             complement(1477..1881)
                     /locus_tag="OO179_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5533764.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="OO179_003881"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="OO179_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5533765.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="OO179_003882"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="OO179_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5533766.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="OO179_003883"
     CDS             complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="OO179_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5533767.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1497 a         1410 c         1495 g         1290 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc ta
//
LOCUS       DAJTNW010000167         5665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNW010000167 DAJTNW010000000
VERSION     DAJTNW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..246)
                     /locus_tag="OO179_003884"
     CDS             complement(<1..246)
                     /locus_tag="OO179_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5533768.1"
                     /translation="MLPPNIRGYAPQITGIAETNARVVVSQQGRVIYDSTVPAGTFSI
                     QDLSSSVRGILDVEIFEQNGKRKHFQVEMCRCAFLIQT"
     gene            complement(432..3740)
                     /gene="icsA"
                     /locus_tag="OO179_003885"
                     /gene_synonym="virG"
     CDS             complement(432..3740)
                     /gene="icsA"
                     /locus_tag="OO179_003885"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5533769.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGIIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4269..5471
                     /gene="virA"
                     /locus_tag="OO179_003886"
     CDS             4269..5471
                     /gene="virA"
                     /locus_tag="OO179_003886"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5533770.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1769 a         1275 c          938 g         1683 t
ORIGIN      
        1 agtctggata agaaaggcac acctgcacat ttctacttgg aaatgttttc ttttaccatt
       61 ttgctcaaaa atttcaacat ccaatattcc tcgtacagaa ctactcaagt cctgaataga
      121 aaaagttcca gctggtacag tagagtcata tattactcga ccttgttgag atacaacaac
      181 acgtgcatta gtttctgcaa taccagtaat ctgaggcgca taaccacgaa tatttggcgg
      241 tagcattcgt tcatcgcttt ctaatgcaat tcctgtgtaa cgccaagcaa cagcgcattc
      301 tggcgcatga tctggtaaac acgtttggca ttgatcgcag gcataccatc atgtgcacaa
      361 aaacccaccg agcttttaca cggtgggtcc cagagaaatg caggacatca acacgccctg
      421 catttttatt atcagaaggt atatttcaca cccaaaatac cttgggtgtc tctgtaactg
      481 ttattaccca attgctgaga tacgtttccc cagatgctta ggttatctgc aagacgcccc
      541 cccacaccag ttttgagttc aatcacatta cggttgccta tctggtgatt tgacatgcca
      601 ttcataatta taccatattg ctttgagctc catttccaat tcacttccaa aaatggttcg
      661 aacttacgaa gagtctcctt atcgatacaa ctctgactaa ttaaggatgt acgcaatccc
      721 aattttgctt gaatattatt gcccccagag gatgtgacca tagtgccatt gtgttcaata
      781 tagtcgtttg cttttacccc atgcctagtt atctggactt gtggattgag atacaatgcg
      841 ttgtcaatat tattatgagc agtccagcgt atggttgggt agatatagcc agcttccaaa
      901 gctcctgtta ttcctttgct ggaatatttc tcaccagtta acccatctcc tttgactgta
      961 ttattaaacc aactgtactg caaccaagca tccatatata gacctgttcg ttctttttca
     1021 tcctgaaacc aagaactgta caaaccggca gtataaccgc tcacagttcc tcgtgaacta
     1081 taactagtat acttattatg agttttgcta tcctgatatc cataagctcc cataataccc
     1141 acatgcaagc catcatgatc agtgaagttt gtcttcaaaa tatctccacc aatctgataa
     1201 accatcctgt tagctgtggt ttttaattgt ctatcagcta aattatgctc attatgtcca
     1261 ccaacagtac gaatccacat ggtggtttcg ctgagctgac ctgtagtagg atcaacaata
     1321 gtgctaccgt gtcggtcata gagagaagga ctaaacatcg tgttcattgc tatcaggtta
     1381 gcaatataac tgccagcttc agggcgccat gtgtgaatac taccttcacc caaattaagt
     1441 gaagatacaa gctgtgaact agctcggcga ctactcattt gagtagactc ttgattgctc
     1501 atctgttttg attcttgatt atccatctga ctagttagat accacttatt ggtattcagt
     1561 ccagatacag tgccctgttt caggcgatag tcataactac cagcaacaat acggcctttc
     1621 tgaataaaag cattcttatc agaagaatct gtcgaaataa tttgaacacc ttcaagagtt
     1681 ttatctccag agcctccctc atttgtcact gcaacatagg taataccaga cgcatggcct
     1741 gcaatttcca gccggtcagt tttactgttg tcattgccga gaaccgcgcc caaagataat
     1801 attccgccct ttccatgcca attacctttc tgaacatagg tctgaccgac atttgacgaa
     1861 ctattattga gaataacatg accgtttttc tccagagtca tgttacctgt gacaatgacg
     1921 gggtcaggca aaaaaggggc attaatatta ttgattagca cagtcccact atttaataaa
     1981 gtgttaacag tttggttcat gcctgaaaaa ttcaaggtag cacctttatt aacaataaca
     2041 ccagcggtac gtgtcatagc ttcaactgtc cccattttta gaatgcctgc attgatgttg
     2101 gtgaaaccag agtaggtatt tttttccgcc aaaatgagag ttccctcccc tgattttgtt
     2161 agggattttc cgtcccagct atttatatta tgatggctga gattatctgc cagaatagta
     2221 ctaacagtaa gttcagcgtt ttctttcaga ttgaactctc catgactgtc gccatctgta
     2281 tcattccagc tcaaaccgta gatcaattta ttctggtcac tgatatagct ttttgtatag
     2341 tcaatagcac cagtagtgag taacggattg atgatagata tattagaaac actgaaacct
     2401 gttatccctt tatctgcact tagcagttta tagtccttct gcgcccagtt atttataata
     2461 gcctccactt ttacattgtt gccaaatgac atatttccag aagcttccag actactgatt
     2521 ccagctaaat ttaaagaact gttctcacca aaagaaacgt cacctgaaac agtgaatttg
     2581 ttacctgata gtgaaacagt tgtacaatca ccagcacata aatcaccttc aatagttggt
     2641 gtggtcccag tgtaatcatt gttgataatt ttcagaatgg atgaattgtt aacctgtaca
     2701 tcaccagtaa tgacagaacc ttcatggagt attaatgaat tgtttccacc agtgatgtgg
     2761 atagcattac ctgctacatt ttgtgttcca tcatcttctt tacctgaaat tatatatccg
     2821 ctattagcaa tggtcatatt gctaccattt atagcatccc cccaatgtcc accattaccg
     2881 cctaaaatag ctccattgtt gattacagac aggttatctc ctgtgatagc attaccaccg
     2941 tagccatcat aaccataacc gtaagcagca cctccgttgc ctccagcgaa cgtgccctta
     3001 ttgattacag agaggctgct tcctgtgatg gcatcacctc cattaccgcc atcaccctca
     3061 ccataattgt tacctccatt accaccggaa atagtccctt tgttgattat gggtagattg
     3121 actcctgtga cggcatcacc tccattcccc ccatcactat cactataact atcgccacca
     3181 tgtccacctg aaataatttc tccatttatt ataaacagat tgtctcctgt aacagcctca
     3241 ccaccatcac cgttatggtc agcaccgcta ccgccgctac caccaagaat catgccttga
     3301 ttgattatag acaagtcaga tccggtaata gagtcaccac cattaccgcc acaactatta
     3361 ccatcattat tatcaccagc accaccatga cctgcgctaa tcataatatc agagttaatc
     3421 cgtagcttag ttctagatgc atgagagggg ttggaagaag ttatatacca cgcatccatt
     3481 ccatcttcac cagctcccaa gggagaaagt ccatcaacag gtgttaataa tttttcataa
     3541 ttgtcctctg aaaaatgaag ttcttgagta cccgaaagag gagtagcaaa agctattggc
     3601 cccccgagca acagggatgc accaacaaca aaaggagaaa aagacaattt gcatgttttt
     3661 tcctttaccg taggtaattc tccggccccc ccctgtgaac attgggtcat attacaaaaa
     3721 aatttgtgaa tttgattcat gcactatatt atcagtaagt ggttgataaa cccctgaaaa
     3781 gattccgttt tatccggaat aaagggacga tatatgcaaa aacatattaa acaaagcctc
     3841 aaccaaaaca tgagagctgt gttatatatc aatgcaacgg gagaaattta cctgtgtctt
     3901 gctttgaaaa aaagaggggg aaagctatat gttcgataat aaattaataa ttttactatg
     3961 atagtctgct tcagaccgca acttttcagt tctttctgta cgcttgccca aagtttaacc
     4021 actttgtttt tctatacacc caatggttct atctgcccct tgatgttaat ccccaaagcc
     4081 aataacatga atgtattcat atcacaactt tcctaccaga ctctccaatg caataactgc
     4141 ataattgttt ttagtggaaa tgtatcgttt tcttaaagag aagaataaca ttccatttta
     4201 ttatgttccc aatttaatta tattaatata tattggcatt aataggaaaa tacatcagga
     4261 gaaatcaaat gcagacatca aacataacta accatgaaag aaatgactca tcatggatgt
     4321 caactgtcaa atcaactaca gaagtaagtt ggaataaatt gagtttttgt gacattttac
     4381 tgaaaataat aactttcggc atatattctc cacacgaaac tctagccgaa aaacatagcg
     4441 aaaaaaaact aatggatagt ttctcaccat cactatcaca agataaaatg gacggagagt
     4501 ttgcccacgc aaacatagat ggcatttcaa tacggctatg ccttaacaaa ggcatatgtt
     4561 cggtttttta tcttgatgga gataaaatac aatccactca actttcatca aaagaatata
     4621 ataacttgtt aagttcactc ccccccaaac aattcaactt aggaaaagta catactatta
     4681 cagctccggt atcagggaat tttaaaactc acaagcctgc accagaagtt attgaaacag
     4741 ccattaattg ctgtacatcg ataataccaa acgatgatta cttccacgtt aaagacactg
     4801 attttaatag tgtttggcat gatatttaca gagatattcg agcatctgac agcaatagta
     4861 caaaaatata tttcaacaat attgaaatac cactaaaatt aattgcagat ctaattaatg
     4921 agttaggaat taatgaattc attgatagca aaaaagagct tcaaatgcta agttacaacc
     4981 aagtaaataa aataatcaat tcaaacttcc cccaacaaga cctatgtttt caaacagaaa
     5041 aacttttgtt tacttcactc tttcaagacc ctgcatttat atctgcacta acatctgcat
     5101 tctggcaatc tcttcacatc acgtcttcct ctgtggaaca catatatgcc caaattatgt
     5161 ccgaaaacat agaaaacagg cttaatttca tgcctgaaca acgagttatt aacaattgtg
     5221 gacatattat aaaaatcaac gcagttgtcc ctaaaaacga cactgcaatc tctgcctctg
     5281 gggggagggc ttacgaagtt agctcatcaa tattaccatc gcacataaca tgtaatggtg
     5341 ttggtattaa taaaatagaa acaagctatc tcgttcatgc tggaacactt ccaagcagtg
     5401 aagggcttcg caatgctatc cctccagaaa gtcgtcaagt atcatttgcc atcatatctc
     5461 ctgatgttta agccaatatt atattggctg ccagactgta aaaaaacgtt cttcagaaca
     5521 gctctataaa ctattaaacc aaagctaaaa ggatggggac caatagaatc tcagcccatc
     5581 ctttttatat cattatgaac cgccccggaa atcctggaga ctaaactccc tgagaaagag
     5641 gtaaacagga tgactaaaaa tacac
//
LOCUS       DAJTNW010000168         5584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNW010000168 DAJTNW010000000
VERSION     DAJTNW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.168"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            28..243
                     /locus_tag="OO179_003887"
     CDS             28..243
                     /locus_tag="OO179_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5533771.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="OO179_003888"
     CDS             236..622
                     /locus_tag="OO179_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5533772.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="OO179_003889"
     CDS             630..1316
                     /locus_tag="OO179_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5533773.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO179_003890"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO179_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5533774.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO179_003891"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO179_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5533775.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO179_003892"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO179_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5533776.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4043..4414
                     /locus_tag="OO179_003893"
     CDS             4043..4414
                     /locus_tag="OO179_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5533777.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4424..>5584)
                     /locus_tag="OO179_003894"
     CDS             complement(4424..>5584)
                     /locus_tag="OO179_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5533778.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1031 c         1215 g         1796 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 attc
//
LOCUS       DAJTNW010000169         5522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNW010000169 DAJTNW010000000
VERSION     DAJTNW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..166)
                     /locus_tag="OO179_003895"
     CDS             complement(<1..166)
                     /locus_tag="OO179_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCR5533779.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTS"
     gene            complement(311..814)
                     /gene="cheW"
                     /locus_tag="OO179_003896"
     CDS             complement(311..814)
                     /gene="cheW"
                     /locus_tag="OO179_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5533780.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(835..2799)
                     /gene="cheA"
                     /locus_tag="OO179_003897"
     CDS             complement(835..2799)
                     /gene="cheA"
                     /locus_tag="OO179_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5533781.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLARNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2804..3730)
                     /gene="motB"
                     /locus_tag="OO179_003898"
     CDS             complement(2804..3730)
                     /gene="motB"
                     /locus_tag="OO179_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5533782.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3727..4614)
                     /gene="motA"
                     /locus_tag="OO179_003899"
     CDS             complement(3727..4614)
                     /gene="motA"
                     /locus_tag="OO179_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5533783.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4741..5319)
                     /gene="flhC"
                     /locus_tag="OO179_003900"
     CDS             complement(4741..5319)
                     /gene="flhC"
                     /locus_tag="OO179_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5533784.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5322..>5522)
                     /locus_tag="OO179_003901"
     CDS             complement(5322..>5522)
                     /locus_tag="OO179_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5533785.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1285 a         1502 c         1356 g         1379 t
ORIGIN      
        1 ttgacgtcag ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc
       61 tatggtgaag ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc
      121 ccagcaccat taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg
      181 aaaacaagtt gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg
      241 ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa
      301 atcgggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt
      361 tcagcagttt ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag
      421 tgagatattc tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg
      481 tcaatgaaag cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat
      541 tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc
      601 gtaagtcaac aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg
      661 gcgtgttcgc aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat
      721 caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc
      781 tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc
      841 ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat
      901 cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata
      961 gttactttca aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa
     1021 ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt
     1081 cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac
     1141 ttccagcacc cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga
     1201 ttccataaca gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc
     1261 gtcgaggatg gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt
     1321 cgactggatt tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc
     1381 gacgccgcgc ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat
     1441 cagcattgca acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc
     1501 ttttgccaga attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat
     1561 gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc
     1621 gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtgcca ggtgggtcag
     1681 cgggtcgata atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt
     1741 cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact
     1801 aaagacatat tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc
     1861 gttacgttgt aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc
     1921 gctggaacgc tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa
     1981 ttgatcaacc ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt
     2041 ttcccgctcc acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt
     2101 agctattttt ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa
     2161 acagagtacc gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga
     2221 atccgcccct ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag
     2281 gtcgacttcg ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact
     2341 ttgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt
     2401 ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc
     2461 ggcatccggc tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt
     2521 ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc
     2581 gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc
     2641 ccctcctttg atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc
     2701 ttccggctgc aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc
     2761 aaaaaatgtc tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg
     2821 atggcattgt gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg
     2881 gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta
     2941 ccaacaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg
     3001 ttgccgccat gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt
     3061 cgcggcggga tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac
     3121 cgctggcgta gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt
     3181 tcagcacagg cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc
     3241 cggttctaaa catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga
     3301 ccagatcgat tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact
     3361 ggtcgagatc accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt
     3421 cgatgttcgg ctgcttattc acttccccct ggctctgggt gtaatcatca ccaccgccgg
     3481 gaattgggct ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag
     3541 tccggaagta ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca
     3601 tcaccaaaaa aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc
     3661 catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt
     3721 gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg
     3781 ctcttccagt tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc
     3841 aacggcgata ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg
     3901 catcattttg ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa
     3961 tccgtaagcc aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcacccc
     4021 cagctcggcg gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc
     4081 gacaatacca aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc
     4141 tgcctcgctt tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt
     4201 catgtgaccg ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc
     4261 cgcgaggatg cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg
     4321 ttccagcgaa aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag
     4381 cagatccata tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt
     4441 cagcgtgcct ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc
     4501 ggcaataatc accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa
     4561 ataaccgccg aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc
     4621 atccttccac tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg
     4681 gaaaaaaaaa gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag
     4741 ttaaacagcc tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg
     4801 ggaaagttta cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct
     4861 gccaacaggc tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag
     4921 ttgcagtaat ccactttcaa caaaccgcac caatgtcctg gcacgggtta atgccagcag
     4981 tggtccttct tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc
     5041 atcgacgcca ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga
     5101 agcatgaacg ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt
     5161 cggcggtggg cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg
     5221 tgtttcgctt tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg
     5281 aatatcccgc gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc
     5341 agcgcttctt caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta
     5401 tgaatttgct ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg
     5461 ctgtcaaaac ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga
     5521 ag
//
LOCUS       DAJTNW010000170         5472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNW010000170 DAJTNW010000000
VERSION     DAJTNW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            103..1029
                     /gene="tauA"
                     /locus_tag="OO179_003902"
     CDS             103..1029
                     /gene="tauA"
                     /locus_tag="OO179_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5533786.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1042..1809
                     /gene="tauB"
                     /locus_tag="OO179_003903"
     CDS             1042..1809
                     /gene="tauB"
                     /locus_tag="OO179_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5533787.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1806..2633
                     /gene="tauC"
                     /locus_tag="OO179_003904"
     CDS             1806..2633
                     /gene="tauC"
                     /locus_tag="OO179_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5533788.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2630..3481
                     /gene="tauD"
                     /locus_tag="OO179_003905"
     CDS             2630..3481
                     /gene="tauD"
                     /locus_tag="OO179_003905"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5533789.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3587..4561)
                     /gene="hemB"
                     /locus_tag="OO179_003906"
     CDS             complement(3587..4561)
                     /gene="hemB"
                     /locus_tag="OO179_003906"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5533790.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5085..5453
                     /locus_tag="OO179_003907"
                     /pseudo
     CDS             5085..5453
                     /locus_tag="OO179_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1274 a         1380 c         1499 g         1319 t
ORIGIN      
        1 tcggcgcata acttattgaa tcccatcaaa tcccgtcttt ttttacccat tcccggttat
       61 cttgaataaa ttacctagtc atataacaca cttcttgccg cactggcatt catcgctttt
      121 caggcacagg cggtgaacgt caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg
      181 gctcaggccg acaacacctt tgctaaagag agcggggcga gcgtggactg gcgtaagttt
      241 gacagcggag ccagcatcgt gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc
      301 ggttccagcc cattagcggt tgcagccagc caacaggtgc cgattgaagt cttcttgctg
      361 gcgtcaaaac tgggtaactc cgaagcgctg gtggtaaaga aaactatcag caaacccgaa
      421 gatttgattg gtaaacgcat tgccgtaccg tttatctcca ccacccacta cagcctgctg
      481 gcggcgctga aacactgggg gattaaacca gggctagtgg agattgtgaa cctgcagccg
      541 cccgcgatta tcgccgcatg gcagcggggt gatattgatg gtgcttatgt ctgggcaccg
      601 gcggttaacg ccctggaaaa agacggcaag gtgctgaccg attctgaaca ggtcgggcag
      661 tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag attttgccga gaaacatcct
      721 gaggtcgtga aagcgttcgc caaaagcgcc atcgatgcac agcaaccgta cattgctaac
      781 cctgacgcgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg
      841 cctgaaggtg acgttccggg gctggtgaag ggaaatacct atctgacgcc gcagcaacaa
      901 acggcagaac tgaccggacc ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa
      961 gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac ctcgcgcttc
     1021 gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tctatgccga ttatggcggc
     1081 aaaccggcac tggaagatat caacctgacg ctggaaagcg gcgagctact ggtggtgctg
     1141 gggccgtccg gctgcggtaa aaccaccctg ctgaatctga ttgccggttt tgtgccttat
     1201 cagcatggca gcattcaact ggcgggtaag cgtattgagg gaccgggagc agagcgtggc
     1261 gtagtttttc agaatgaagg gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc
     1321 ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca ccagatgctg
     1381 aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa
     1441 cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt attactcgac
     1501 gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa
     1561 ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga agaagcggtg
     1621 tttatggcga ctgaactggt tctgctttca tccggccctg gccgtgtgct ggagcggctg
     1681 cggctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat caagtccgat
     1741 ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca acgggaggcg
     1801 ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc
     1861 cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc actgtatggt
     1921 ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg caacaagtac
     1981 tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg ctgtggcagc
     2041 atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg gtgatcggta
     2101 ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg gatccggtaa
     2161 tcgaacttta tcgtccggtg ccaccgctgg cttatttgcc gctgatggtg atctggtttg
     2221 gtattggtga aacctcgaag atcttactga tctatttagc gatttttgcg ccggtggcga
     2281 tgtcggcgct ggcaggggtg aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc
     2341 tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa
     2401 tcctcaccgg attacgcatt ggtctgggtg tgggctggtc tacgctggta gcggcggagc
     2461 tgattgccgc gacacgcggt ttaggattta tggttcagtc agcgggtgaa tttctcgcga
     2521 ctgacgtggt actggcgggg atcgcggtga ttgcgattat cgcctttctt ttagaactgg
     2581 gtctgcgcgc gttacagcgc cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg
     2641 tctgagcatt accccgctgg ggccgtatat cggcgcacaa atttcgggtg ccgacctgac
     2701 gcgcccgtta agcgataatc agtttgaaca gctttaccat gcggtgctgc gccatcaggt
     2761 ggtatttctg cgcgatcaag ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt
     2821 tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt
     2881 gctggatacc cataacgata atccgccgga taacgacaac tggcataccg atgtgacatt
     2941 tattgaaacg ccacccgcag gggcgattct ggcagctaaa gagttacctt cgaccggcgg
     3001 tgatacgctc tggaccagcg gtattgcggc ctatgaggcg ctctctgttc ccttccgcca
     3061 gctgctgagc tggctgcgtg cggagcatga tttccgtaaa tcattcccgg aatacaaata
     3121 ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt
     3181 gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga
     3241 aggctttact acgcgaattg tcgatgtcag cgagaaagag agcgaagcct tgttaggctt
     3301 tttattcgcc catatcacta aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga
     3361 tattgcgatt tgggataacc gcgtgactca gcactatgcc aatgccgatt acctgccaca
     3421 gcggcggatt atgcatcggg cgacgatcct tggggataaa ccgttttatc gggcggggtg
     3481 atacgagtgc ggacggttcc ctcgccccct tggggagagg gttagggtga gggggcgttc
     3541 actgtacttt caacaggtta actccccctt tctgagagga aaaaaattaa cgcagaatct
     3601 tcttctcagc caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa
     3661 ttgaacctaa gctttcgagc acgactttct cttcatctat agcacccgcc agcgcggcga
     3721 acttaatcat cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt
     3781 cacgcagctc acgcacgatg tcgaggtacg ctccagcagg tttaaccatc aggcagtctg
     3841 cgccctgggc ttcatccagc agtgactcac gaatcgcctc gcgacggttc attgggttca
     3901 tctgatagct tttgcggtcg ccttttaatg cgcttcctgc agcttcacgg aacgggccat
     3961 aaaaggaaga ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagcctgcag
     4021 cgtccagcgc ctgacgaatc gcctgtacct ggccgtccat cgcggcggaa ggggcgatga
     4081 agtctgcgcc tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt
     4141 cgttgtcgac gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga
     4201 aacaagtgtc tgacatgacg atcatttctg gcacagtctg cttgcagatg cgcgacatac
     4261 gcgccaccag tccatcttcc cgccaggcgt cgctgccggt ttcatcggta tggtgagaga
     4321 tgccaaaagt cattacggaa cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca
     4381 gatgtttctc tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa
     4441 tttcttcttc aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa
     4501 acatagcgcg cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aagtctgtca
     4561 tggtctgcct gatgtttgtg gaattaaggt gcacagtata cctgaagcgg ggcaggagtg
     4621 ttttacgaaa gttgtcgcga tgttgacaag aagagaatga aagagagacc aggacatctt
     4681 tgtaatagaa atagcaataa tttcatattc actgaaaata tttttaatct ttatttatag
     4741 ctcttggtta ttattttttg gcgtttggtt gtgctgctat aaattgaata attcaaactt
     4801 tgttgcatat tgataagata tttatctggt ttgtgttttt gatatttata acgcgtacgc
     4861 atcaaaaata acattatctg caacatactg aaatatcgtc cttttatcaa tttgatgcgt
     4921 gtttttctcc ataaattata tatgtccaca ttcggactta ggggaaagaa taattgaacc
     4981 attcgtctgt aacgcagcat aatcgttagc gcgaaacata atatgttttc tatgcattga
     5041 taattgatgg atcaacttat tacgtccctg aggagggatg acaaatgcac tcctggaaaa
     5101 agaaacttgt agtatcacaa ttagcattgg cttgcactct ggctatcacc tctcaggcta
     5161 atgcagcgac caacgatatt tctggtcaaa cttacaatac tttccatcac tacaacgacg
     5221 ccacctatgc tgatgacgtt tactatgatg gttatgtagg ctggaacaac tatgccgctg
     5281 atagctatta caacggcgat atctacccgg tcattaataa cgctaccgtt aacggcgtga
     5341 tttctaccta ctatctggac gacggtattt ctaccaatac caacgccaat agtctgacaa
     5401 tcaaaaacag cactattcac ggtatgattt attccgagtg catgacaact gattagactg
     5461 gccccctgaa tc
//
LOCUS       DAJTNW010000171         5429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNW010000171 DAJTNW010000000
VERSION     DAJTNW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            188..445
                     /locus_tag="OO179_003908"
                     /pseudo
     CDS             188..445
                     /locus_tag="OO179_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OO179_003909"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OO179_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5533791.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OO179_003910"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OO179_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5533792.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OO179_003911"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OO179_003911"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5533793.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="OO179_003912"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="OO179_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="OO179_003913"
     CDS             complement(3603..4418)
                     /locus_tag="OO179_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5533794.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..5162
                     /locus_tag="OO179_003914"
     CDS             4515..5162
                     /locus_tag="OO179_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5533795.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVHV"
BASE COUNT         1427 a         1265 c         1282 g         1455 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcacgtat
     5161 aggaaattga aaaaccagac gataccgctg tccacgcctt atgccacgtg agcttttctg
     5221 cttttccctc cactccggtg ttttctctct ctgcatggtc gattcttata ccgcaacagg
     5281 ctgtttttta tacttgcacc gattcccggg cacacttagc ctgtgtcagg ccggcatata
     5341 cctcagcaga ctgatatccc gcgcgttttt cttcagcccc tccacattca gtcccgcgat
     5401 ggcccggcgg tataccatcc ggccgccac
//
LOCUS       DAJTNW010000172         5406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNW010000172 DAJTNW010000000
VERSION     DAJTNW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            862..3147
                     /gene="nrdA"
                     /locus_tag="OO179_003915"
     CDS             862..3147
                     /gene="nrdA"
                     /locus_tag="OO179_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5533796.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3381..4511
                     /gene="nrdB"
                     /locus_tag="OO179_003916"
     CDS             3381..4511
                     /gene="nrdB"
                     /locus_tag="OO179_003916"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5533797.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4511..4765
                     /gene="yfaE"
                     /locus_tag="OO179_003917"
     CDS             4511..4765
                     /gene="yfaE"
                     /locus_tag="OO179_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5533798.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4819..>5406)
                     /gene="inaA"
                     /locus_tag="OO179_003918"
     CDS             complement(4819..>5406)
                     /gene="inaA"
                     /locus_tag="OO179_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5533799.1"
                     /translation="EEPNYRRNGMSGVQCVERNGKKLYVKRMTHHLFHSVRYPFGRPT
                     IVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALLVTEDMAGFISIADWYARHAVS
                     PYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIYVKTEGKAEAGFLDLEKSRRRL
                     RRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1382 a         1417 c         1300 g         1307 t
ORIGIN      
        1 cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg atatcatatc
       61 cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat gcaatcattg
      121 acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta atctgaccat
      181 cattttatat aagacggcat atttaaacgc attatacaat atgggttatc tttcttttgt
      241 aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa tatcagtgcg
      301 tcataattca agttaataac cttaagggat accagttata tttaaactaa attaaagtaa
      361 tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt taacaataac
      421 cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat atattgacca
      481 caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc aatcattagc
      541 aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt tcaagaaatc
      601 gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg acaccttgtc
      661 tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa gttatgcact
      721 tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc cctatatata
      781 gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc ggaaagaatt
      841 ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg cgacggtagc
      901 acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc agaaggactg
      961 cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta tgacggtatc
     1021 aagacctctg acatccacga aactattatc aaggctgccg cagacctgat ctcccgtgat
     1081 gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc
     1141 tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc
     1201 aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca gatggacacg
     1261 tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca gctggaaggc
     1321 aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca gttcctttat
     1381 attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct gcaatacgtg
     1441 aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc
     1501 ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg cggtgacagc
     1561 ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca gcgtgccggg
     1621 atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg tggcgaagcg
     1681 ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa atcctgctct
     1741 cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca tctggaagtg
     1801 gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt gcgtcatatg
     1861 gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg tgaagatatc
     1921 accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc cgatcaggaa
     1981 gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg
     2041 aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg tcgtatctat
     2101 attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat cgcgccagtt
     2161 cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa cgacgtcaac
     2221 gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat
     2281 agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga cgcgctactg
     2341 gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg tcgtacgctg
     2401 ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg ctactccgac
     2461 ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta cctgctgaaa
     2521 gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga aaccacttac
     2581 gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat cgctaatgag
     2641 ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg cctgcgtaac
     2701 tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa cgccactaac
     2761 ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg tattttgcgc
     2821 caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg ggaaatgccg
     2881 ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga tcagtcgatc
     2941 tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc gatgcagcag
     3001 ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta ttatcagaac
     3061 acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca ggacgatggc
     3121 tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt aaacgcctta
     3181 tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca tcaggctccg
     3241 ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt aggcctgata
     3301 agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat ccggcattaa
     3361 actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa aatgatcagc
     3421 tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat cagcaaaaat
     3481 atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt ccggaagaag
     3541 ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa aaacacatct
     3601 ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt agcccgaacg
     3661 tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa acctgggcgt
     3721 tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc gttaacgatc
     3781 cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt gcggaaggga
     3841 tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt ctgggcgaag
     3901 gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg aagaaaaaac
     3961 tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac gtcagctttg
     4021 cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag attattcgcc
     4081 tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg aatctgctgc
     4141 gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag caggagtgct
     4201 atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat ctgttccgtg
     4261 acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa tacatcacca
     4321 atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc aacccgatcc
     4381 cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag gaagtggaag
     4441 tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat ttgagtaact
     4501 tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc tgtgccagga
     4561 tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg agtaccagtg
     4621 ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg tggactggat
     4681 tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt gccgggcaaa
     4741 aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg cttacgcgta
     4801 ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct cccagtccgc
     4861 tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa tcgctttatc
     4921 gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg ccttaccttc
     4981 tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa cgctatgcat
     5041 tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt cagaataagg
     5101 cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca tatcttcagt
     5161 caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa cgatcttcgg
     5221 cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga caatcgttgg
     5281 tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct ttacatacag
     5341 ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc gatagttagg
     5401 ctcttc
//
LOCUS       DAJTNW010000173         5400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNW010000173 DAJTNW010000000
VERSION     DAJTNW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..497
                     /locus_tag="OO179_003919"
     CDS             <1..497
                     /locus_tag="OO179_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5533800.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO179_003920"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO179_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO179_003921"
     CDS             2500..3114
                     /locus_tag="OO179_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5533801.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO179_003922"
     CDS             3114..3443
                     /locus_tag="OO179_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5533802.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO179_003923"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO179_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5533803.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="OO179_003924"
     CDS             4530..4841
                     /locus_tag="OO179_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5533804.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..4983
                     /locus_tag="OO179_003925"
     CDS             4825..4983
                     /locus_tag="OO179_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5533805.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4976..5374
                     /locus_tag="OO179_003926"
     CDS             4976..5374
                     /locus_tag="OO179_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5533806.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1181 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
//
LOCUS       DAJTNW010000174         5370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNW010000174 DAJTNW010000000
VERSION     DAJTNW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.176"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..763)
                     /locus_tag="OO179_003927"
     CDS             complement(<1..763)
                     /locus_tag="OO179_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5533807.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPT"
     gene            complement(778..1482)
                     /locus_tag="OO179_003928"
     CDS             complement(778..1482)
                     /locus_tag="OO179_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5533808.1"
                     /translation="MRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIMDALERYSEIIT
                     PWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMKSIVAEQIKYIK
                     SLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANLIARTELGRATG
                     ALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGMTGHAGELPNC
                     RCYKEIVFPNPHSYLA"
     gene            complement(1403..2878)
                     /locus_tag="OO179_003929"
     CDS             complement(1403..2878)
                     /locus_tag="OO179_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5533809.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(2878..4500)
                     /locus_tag="OO179_003930"
     CDS             complement(2878..4500)
                     /locus_tag="OO179_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCR5533810.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(4503..5075)
                     /locus_tag="OO179_003931"
     CDS             complement(4503..5075)
                     /locus_tag="OO179_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5533811.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(5059..>5370)
                     /locus_tag="OO179_003932"
     CDS             complement(5059..>5370)
                     /locus_tag="OO179_003932"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5533812.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         1125 a         1537 c         1363 g         1345 t
ORIGIN      
        1 cggttggacc tccgcccatc tctggtactt tgtccggtag cggttgctgc ggggacaggt
       61 tgatgttgag attaacgccc tgcggcaaat ccccctcatc tcctgtaacc gatgcgggag
      121 ccgactccac cagttcgttc atggtgtcag cgtcacccgt tttgatggcc gtgcgcatgc
      181 gggtccacca gcttttcttt tgatttgcca ttgtgtctct gtctccaatt gcacaacgat
      241 ttccggctct gcctttgggg acaagagcca catggtttcc ggtaatatcg acctgctcgg
      301 ctttacctgg ctcggtctgc tcgtactccg cgtcatagcc gcacgacact tcacgcaggc
      361 catcttcgat aagctgaatg gcgttttcgt ctttgacgat aaggtcagcc agcatcaaat
      421 cagagtggtc acccgtcccg cgccgcacat tctgaagatg cccgacagca agctctttcc
      481 agttctcggg atttaccagc cgcacattcc cgttttcatc ttcaggatgc aggatcgtga
      541 tgctcatccc ttcgaatgag gcgagcgtgg ccggatggaa tacctgctca ggagagcgcg
      601 ttacgactat ctcaccgagc ttgtcgggtt tgaggtttgg cagatcggca gcgccgtaga
      661 gctgtttacc cgttcgacct atcggcacgt ctttgcacag cagcgagccg tcagccagct
      721 gatagcgggt ttcccccagc cgggtattga aaaaatattt catggtttac ctgcgattca
      781 ggcgagataa gaatgagggt tgggaaaaac gatttcttta taacagcgac aattcgggag
      841 ctcgccagcg tgaccggtca tgccatcaag cgttggaggt ttgccccatt cgacaaactt
      901 accttccatc tcccgatgag aatgccggac gtcgccatct tcggctgtac gccagatata
      961 accattcgag ccgattgaca gcgcacgcgc ctgatcgagc gcgccggttg cacgtccaag
     1021 ctcggtacgg gcgataaggt tcgctcgtga gcgtgacacg tcaccggacg ctgctatctc
     1081 tttcgcgaat ggttcagcgc ggccaccagc cacaacggcc tcgatggcct tgttctgaat
     1141 gtcatacacc cgatcggcgg cctcaagagg cagtgacttg atatacttaa tttgctcggc
     1201 gacgatggat ttcatcacct ggcctaccgg ggcgcggtcg accatgttgc gcagctctgc
     1261 gctgatgttc cggctgtgct gacgccactg cttttcattc tggcgtgcaa tgtcggcggt
     1321 aaagttctca gcaaccttcg tcgcccaggg ggtgatgatt tcgctgtagc gctccagcgc
     1381 atccattatt tcggtaacgc tatcatttga accatcgtag cgcccattta cgatatcccc
     1441 gaccgcccgc gctatctgcc gtaggctcgt tcgatatcgg atctccgcct ggcggctctg
     1501 gcggctctgg cggtttgtcg ccaagttcgc cgatgcctgg cggcgcttcg tcttcggcat
     1561 tctcgatatc ctcgtcggta atggatgccc cgatgccggt gacgtcagag ttttcgcgca
     1621 ggtcggtcat cgccgcctta cgcgtcatca atccgtcgcc cagcgcggta ctaatcgcgt
     1681 tggtggtgtt tacggccacc gttgatcggt caacgtctga catttgccat agcgggttaa
     1741 actcaaacgt gaaatcgtcc ggcagcggct ttccgagttc cgagcggtgc ataatgtcca
     1801 gtatccggcg catcggcagc cgtaagcggc gctcctgcaa tgagctcacc cggtcgtaat
     1861 agttggcgag gtctgcatca ccagtagaga agcctttcgg ggattgaccg aacaaccgca
     1921 ccagcgggat accaacggca ccgctaatct gctcagcaaa ctgcgaaaga atgtcatcca
     1981 gaccactaaa gctgtactgg tgggtttcga acttatcccg cgagtccatg agcgtcatac
     2041 cttcattgct ctggaactgg cggatcaggt cgatgttctt cagcaacgcc tcgaacgccg
     2101 ggcctccaag cgcgataagc tcgcgcaact tctccacgct ataggtacgc agatgtgctt
     2161 tgtagaccag ctgcgccgcg ccgacagtgg cgctgtcgaa cgcagtaagc cgatcccaga
     2221 tacgctctac aaccgacatt ccccattcgt tttcggtcat cttctgctgg aatggcagcg
     2281 tcaccccgtc gaagcgaatc aggcggctgt gatggatgcg ccaggccggg atgcccgttg
     2341 cagtggtcac cacgtcgtaa aactcaggtt tgccgagatc tggccccatc tctttaatgc
     2401 ggcgggtcag gaccgggtta atcatccagc ggtcgagcgg gagaatgccc ttaaacttgc
     2461 cttctccaat ggtttcgagc cgcagcgggg tcattggtgc ctgcccctcg atcatgatga
     2521 agccgaccgc gccgccgtag aggcgagacc atttcagcac gtcgttcagc gcatcccaga
     2581 tttgcaactc atccagttgt gattcgagaa tgccacgatc ttttgcatca atttccgaag
     2641 tgatgcgaat gcctttgcgg gtcatatcat ccgggatagc atcgaccgct tcgccgataa
     2701 tccaggacga acgataggac cattccacca gcatgcggtt acgactggtg aaattagccc
     2761 ggtaggtgga tgctgagtgc tggttaggtg tctgcatccc cacgcgggca acaaagttct
     2821 catagccatc agcggtggcc tgtgccgttc gccgcgtggc ttgtttgttt cgtgccatca
     2881 ggcctgtctc cctagcagct cccagatgtt cagggctgaa ttcattggcg cgtagctgat
     2941 catcaccgag tcggcaaggt ttggcgaccg ggttccatca ggctgtttat caataacgat
     3001 ttttcccaca ccattaatgg aataggtcgg ctgcgaaagc tcgatgatga gtttatcttt
     3061 gagtgccatg ctactgctga ttgagatgat ttcgtccggg ttgtaatcca taccttcaac
     3121 cacggcgcgc caggtattct gaaaaagttt acgtaaccgc caccagctct gggctttggc
     3181 gttagcgaag aagtccttgt tcaggcgggc ggcctgtccg ttgtcaccgc gcaccgcttc
     3241 gtcgtccgga tcaaataccg cgccactacc tcgaaacggt gtggcgagta ttgacggtcg
     3301 gcgcgcagcg ttacgcagtt cgttgatagc gcgtgcatcg ccgcgaacgc cagcgcccaa
     3361 cccgtcctcg tcaaagcgaa actcttcgag gttgtcctgt tcgcaaaagc cgaaaacctt
     3421 ctcgacggac tgataaatgt cgctgcccac accggaccat tcccgcacat tctccaggag
     3481 gaagccatga cgggtggaaa aggaattttt gtccctgcct tcgtcggcga cgtccatcgc
     3541 gcccagtcgc ttgcctgttg gctggatgcc cagcttgata tgcgcgtcga cagcagcctg
     3601 tacccattcg gatggaatca ggacgccttc cgctgatgca ctgtagttca ggtcaagttc
     3661 ctgtgccacc accaccggat tgtcgatttt ctcgcattcc ctgcgatacc actcttcatc
     3721 cttgcgagga tcatcccgcc agtggaatgt gaataccggt atcttcccgc catgacgctt
     3781 ctgagcgaac gggttcgcca tgccgttaac tgaactcagg tcaatacggc aacgcgtcgt
     3841 ttgtgacaac gccgcatcaa tcagcagagg acgctgaagg aatgcagact catcaaccag
     3901 ataaagcgtg gtacggtcac cacggccaat attatcgcca gcctcacctt tgataacggc
     3961 accagtttca ggaaactcaa cgcgcatgta tggtgcgtgc ttcttttcgt cccacgaacc
     4021 tcgaaactct acaggtagtg tttccacgaa cttgcgcgcc ttccagaaca atgctttcgg
     4081 gtcaccggtg ctgtcgacgt attcctcttt acgggagccg aaaccgataa ccatttcttt
     4141 gttgaagaga caaagcgagc atgccagccc gatcgcggtc caactgagcc ccatttcacg
     4201 ggatttttcg gtaataccat tctcccgatt accccagcgt tccataatcc agtggatcca
     4261 ctcctcctgc ttagggaaga gtaaaaacgg aatggtcacc ggcaggccat aatcaatatt
     4321 acgcgggtcc gttgtcatgc cccagtcgat gatgaactga gccggattgg ttcggtaaaa
     4381 ctgttttagt gcaggcaata tttcaggatt ctggcgaatg cgctgtaggc gttccatccg
     4441 ccattcaaaa accatctggt aatcaggatg tttaaaatcg aaggggaatg gtaacggcat
     4501 acttagccca tcatttttct atacgcctct gcagcctgct ccggcgttaa gttggtaatt
     4561 tctgttctga ctggtcctcc gtcagcacca gtcacttcat ttttgacatt gtctttaaac
     4621 gcctgaacag aaacatgacg cccgagcaac tcaaggtttt taaccttatc aggccatttg
     4681 attttcttca ggagagcagc actatctgct gatgccatct cgacgacatc cattcctgat
     4741 agcgttgtac gccacacctt aggccagtct ttaatctgct tcagttcgcc attggcaagc
     4801 agaatgtcaa gcacgtccat ctgatcgatt tcagtcagcc gtcgcaatac ataggcggcg
     4861 tctataccta cctcttcatt gcgttcagcc tttagctcag caatgcgatc tgctatgtca
     4921 ggttttgaca ggttctcgct accagtggcg cgagcagttt tctcgctgta acccgccctg
     4981 atagctgcct gagtggcgtt taaatctttt aaatattccc tgcagaacag ttcctgctta
     5041 ggtgtgagct gtttcaaatc acacctccga aacattgctc caatttctat gattaacgat
     5101 gtcagcaatc atggtagtgg atacgccata tctctttgct aaagacctaa gcccattacc
     5161 tacactcccg cgcacatact cgcggcgaat gcagatgaca tcgcactctt ttaatttcgc
     5221 cattccgttc ttttcacctg aacggtcagg gtatgagttt ctgcctcgcc ttgacctgtc
     5281 acgattattg tcagcgacag tacctataat cagatggtca ggattcacgc atgatggatt
     5341 atcgcattta tgcctaacaa caaggtcctt
//
LOCUS       DAJTNW010000175         5339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTNW010000175 DAJTNW010000000
VERSION     DAJTNW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO179_003933"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO179_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5533813.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO179_003934"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO179_003934"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5533814.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO179_003935"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO179_003935"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5533815.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO179_003936"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO179_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5533816.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1311 a         1290 c         1455 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg caacgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAJTNW010000176         5106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNW010000176 DAJTNW010000000
VERSION     DAJTNW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..275)
                     /locus_tag="OO179_003937"
     CDS             complement(<1..275)
                     /locus_tag="OO179_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533817.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPAT"
     gene            complement(343..2292)
                     /locus_tag="OO179_003938"
     CDS             complement(343..2292)
                     /locus_tag="OO179_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5533818.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2377..2712)
                     /locus_tag="OO179_003939"
     CDS             complement(2377..2712)
                     /locus_tag="OO179_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5533819.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2712..3068)
                     /locus_tag="OO179_003940"
     CDS             complement(2712..3068)
                     /locus_tag="OO179_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5533820.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3068..4564)
                     /locus_tag="OO179_003941"
     CDS             complement(3068..4564)
                     /locus_tag="OO179_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5533821.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVARRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4561..4722)
                     /locus_tag="OO179_003942"
     CDS             complement(4561..4722)
                     /locus_tag="OO179_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5533822.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4732..5073)
                     /locus_tag="OO179_003943"
                     /pseudo
     CDS             complement(4732..5073)
                     /locus_tag="OO179_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1190 a         1507 c         1123 g         1286 t
ORIGIN      
        1 gtcgctggtg atgcattgtt tgcaaaatca aatacaccta tacttttgcc gaattcataa
       61 gaagcttttc tcaatataat cgcaccgctg gcagaagggg atgggcacga tatcctcagt
      121 tgtctgaatc catctgtatc caagtcaacg tcattatctg cagatgtttt aacgttacta
      181 ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca gcaagaggtt
      241 aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt gttagcatgt
      301 tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt taaatggaga
      361 atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca aatcgataac
      421 gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg ttgccggtga
      481 agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt agcgtttatc
      541 gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat agacaaaatc
      601 atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac tgttcagtgc
      661 atcagccaca aaatttttaa caagccaggg atgttctttt tcaaactcct gcgccatcat
      721 gcccagttca agcaatccac caatcagact gatctttccg aggcccttta atttcagccc
      781 tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac gaatacttgc
      841 caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta ctgcctgttc
      901 ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt tcacaaccgg
      961 accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa gcgcaattaa
     1021 tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc gtgtcaatgt
     1081 gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg tttttacgac
     1141 ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca tctcttttat
     1201 gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg cattgatctg
     1261 gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat attctttctg
     1321 cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc gcaacaaatc
     1381 cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca gcgctttcag
     1441 cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt gcagcatggc
     1501 cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc gcaacgagcg
     1561 gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca acataaaatt
     1621 ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg ttgcccccat
     1681 tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa tacgggtcac
     1741 aacatcagaa atctttttcg cacttgcagg accggtattg ccaagatagt taatcttatc
     1801 cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact gcgccatcat
     1861 ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg catcgtcagt
     1921 aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca caatttcggc
     1981 gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct ccgtcattgc
     2041 cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg ccatttttga
     2101 ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg cggttccggc
     2161 acccgctgcg cccagtgaca ggctggcaaa ctccttttta aaacctttca gctggcgctg
     2221 cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct tcagctgaaa
     2281 actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc agttctgtaa
     2341 actgcgaaac agccacccgt cgcagctcca gtggattcag tttccagaac caggcaacat
     2401 tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac gcaggatctg
     2461 catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac acgggatccc
     2521 cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac tgccttcacc
     2581 ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct cgcgcaactc
     2641 cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta tttctgtaac
     2701 gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc tgccgtgcct
     2761 tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac atacaccatg
     2821 ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt gtcgaccgga
     2881 aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt ctccttgtaa
     2941 tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat cggcacttca
     3001 atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt tccggcgatc
     3061 tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc accgcaaaga
     3121 cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc gggttatctg
     3181 catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca ataccggcac
     3241 gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga gtgacaatcg
     3301 cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg tacttgcttg
     3361 tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt tcactgtcca
     3421 gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg gaacgctgga
     3481 tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa agggtctgct
     3541 gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc agctcccccg
     3601 tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca aggcgtctgg
     3661 caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc gtgatatgtt
     3721 gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt tttgcggtat
     3781 agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca ttcatttcgg
     3841 tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag tcgaatgcct
     3901 catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg gcagcaccga
     3961 cttccgtcac cacccgaagc cctgctggca aaatctcacc accaccagaa ccataataat
     4021 tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc agcgtcacca
     4081 cgcctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc gcttccttaa
     4141 tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt acacgggagc
     4201 gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc gtcaccctga
     4261 ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca ccgaaaggat
     4321 ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca caaatctggc
     4381 gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct gcatcgttgc
     4441 tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc gcagaattgt
     4501 tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta aaacttatcg
     4561 tcatgatttt ttagccttat cctgtttaac acgtaccaca tcacccgccg ctatacggcg
     4621 gaaccagtag ctgctttctt ccacatttcg cccttcggaa ggcaaaaggt ctccacgggc
     4681 agggtcagga accgaccgcc ctttcagggg tttcacaaac atgagggact cttaattctg
     4741 agggaaacgc atttccagat ggtgctcaat gtcaccatcc gggccagtac caggatcgat
     4801 gtaatccaca tcaatactca gcagggataa atccggcagg gcattcacgt cctctgcctg
     4861 tcgcgtgtct tcttccgtga tttcatactt cgccgtaaaa tcaaactggt aatacagttc
     4921 gtaacggttc agatccagca gggtgccacc gacataaaca atctcgcccc cttgcgggtc
     4981 cggcatccat ccaagcaggg ctttccagag ttctctccgg acatcatgaa cggcgtcata
     5041 ggctgcccac tgcccttttt catcacgctc attgaggtgt actggcaata gcggacacta
     5101 ccattt
//
LOCUS       DAJTNW010000177         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNW010000177 DAJTNW010000000
VERSION     DAJTNW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO179_003944"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO179_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5533823.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO179_003945"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO179_003945"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5533824.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO179_003946"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO179_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5533825.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO179_003947"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO179_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5533826.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO179_003948"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO179_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5533827.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1415 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtaaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTNW010000178         4963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNW010000178 DAJTNW010000000
VERSION     DAJTNW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO179_003949"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO179_003949"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5533828.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO179_003950"
     CDS             1020..1703
                     /locus_tag="OO179_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5533829.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO179_003951"
     CDS             1700..2161
                     /locus_tag="OO179_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5533830.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO179_003952"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO179_003952"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5533831.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO179_003953"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO179_003953"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5533832.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO179_003954"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO179_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5533833.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO179_003955"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO179_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5533834.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1171 a         1302 c         1238 g         1252 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tcc
//
LOCUS       DAJTNW010000179         4919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNW010000179 DAJTNW010000000
VERSION     DAJTNW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.183"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..382
                     /locus_tag="OO179_003956"
     CDS             <1..382
                     /locus_tag="OO179_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5533835.1"
                     /translation="GVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPMLDLV
                     VPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAIIF
                     IRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(436..1041)
                     /gene="azoR"
                     /locus_tag="OO179_003957"
     CDS             complement(436..1041)
                     /gene="azoR"
                     /locus_tag="OO179_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5533836.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1242..>4919
                     /gene="hrpA"
                     /locus_tag="OO179_003958"
     CDS             1242..>4919
                     /gene="hrpA"
                     /locus_tag="OO179_003958"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5533837.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVD"
BASE COUNT         1212 a         1227 c         1367 g         1113 t
ORIGIN      
        1 tggtgtttat ttaagggcgt ttccacgaca tattccggca cagaatgcgg ttctggacgt
       61 cacctttgaa ttccctcgtg gacgagcgac aaaagatcga ctctatttct gtgtaccgat
      121 gttggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga tgctggaaac
      181 attacgcgaa gagcaaggca gcgttctggt ccattgcgca ttgggattat cgcgcagtgc
      241 gctggtagtg gcggcatggc tgttatgtta cggacactgt aaaacagttg atgaagcgat
      301 tatctttatt cgagccagac gctcgcatat tgtgcttaag gaagagcaca aagcgatgtt
      361 gaaattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta cagtccacat
      421 caagaccgtg tccggttatg cagcaacaat gctgtcgatg gctgcttttg cgtcagactg
      481 tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa atttcacatc
      541 ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt ccgttggtcc
      601 atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg ttaccagacc
      661 ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca ggtcaaaata
      721 gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa cgataacgtc
      781 gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct gctgacgcgg
      841 agtcagcggc gcatcgctcg gacgcagagc gccaaccagt tcgccatcca gtaccggaat
      901 cggatttgca gctaggtcgc gaacggtgat ttcatcagcg gagtgctttt cgctccattg
      961 ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga tgctggattt
     1021 aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatggac gatgccccgt
     1081 tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa tcgaagtcta
     1141 tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat atctatcatt
     1201 taaaagaaaa ttaatcaggc agactactgc ccactaacgt tatgacagaa caacaaaaat
     1261 tgacctttac tgccttgcaa cagcggctgg attcgctgat gctgcgcgac agactgcgtt
     1321 tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc tgatgcacaa caggccattt
     1381 tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg cgtgaagcgg
     1441 cacgaccgga aatcacttat cctgacaatt taccggttag tcagaaaaaa caggacatac
     1501 tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggc tccggtaaaa
     1561 cgactcagct accgaaaatc tgtatggagc tggggcgcgg tattaaaggg ctgatcggtc
     1621 atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa ccgtattgcg gaagagctga
     1681 aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac gtaagtgata
     1741 acacgatggt caagctgatg accgacggga tcctgctggc ggagatccag caagaccgcc
     1801 tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc ctgaatatcg
     1861 attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta aaaatcatta
     1921 tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat gcgccgatta
     1981 ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt gttgaagaag
     2041 ccgatgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac gaactaagcc
     2101 aggaaagccc cggcgacatt ctgatcttta tgagcggcga gcgggaaatt cgcgataccg
     2161 ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt tatgcgcggc
     2221 tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggcgc attgtgctgg
     2281 cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtc atcgaccccg
     2341 gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg ccgattgaac
     2401 ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg tccgaaggga
     2461 tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt accgatccgg
     2521 agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg gggctgggcg
     2581 atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag gatggcgtgc
     2641 gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc tataaactga
     2701 cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg cgtatggtgc
     2761 tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc gcgctctcca
     2821 tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa aaacatcgtc
     2881 gcttccacga caaagaatcc gacttcctcg cgtttgtaaa tctgtggaat tatcttggcg
     2941 agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc gattacctca
     3001 actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag gtggtgaaag
     3061 aacttggcat tccggtcaac agcgaaccgg cggagtatcg tgaaattcac atcgccttac
     3121 tgaccggttt gctttcccat atcggcatga aagatgccga taaacaggaa tataccggtg
     3181 cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag ccgccgaaat
     3241 gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt gctgcgcgta
     3301 tcgacccgga atgggtagaa cccgttgctc agcatttgat taaacgcacc tacagcgaac
     3361 cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact gtttatggtt
     3421 tgccgattgt tgctgcgcgc aaggtcaact acagccagat cgattcggcg ttatgtcgtg
     3481 aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac gcattcttcc
     3541 gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca cgtcgccgcg
     3601 atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc agccacgatg
     3661 taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa acgcctgatt
     3721 tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc agcaagctgg
     3781 attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat cagtttgagc
     3841 caggcgcgga cgcggacggt gtgaccgtac atattccgct gccgttactt aaccaggttg
     3901 aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg attgctctga
     3961 ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc tgcaccaaac tacgccgaag
     4021 cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt gagcgcgagt
     4081 tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat caggtgcccg
     4141 atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta aaagaagggc
     4201 gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg ctgtccgcgg
     4261 tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt cagctgccgg
     4321 aaagttacga acagaagcgt ggcaactata aagtgaaggc gtggccggcg ctggtggatg
     4381 aacgcgacag cgtgacgatc aaactgtttg ataacccgct ggagcaaaaa caggcaatgt
     4441 ggaatggtct tcgccgtcta ctgctgctaa atatttcctc gccgatcaag tatttacatg
     4501 agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc aaagtgctgg
     4561 agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc aatggtggcc
     4621 cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc gaactgaatg
     4681 acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg ttcaatatca
     4741 acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct gatattaaag
     4801 cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc aaacggctgg
     4861 gcgacacgct gcgatatttg caggcgattg aaaaacgact ggaaaaactg gcggttgat
//
LOCUS       DAJTNW010000180         4867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNW010000180 DAJTNW010000000
VERSION     DAJTNW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(28..2940)
                     /locus_tag="OO179_003959"
     CDS             complement(28..2940)
                     /locus_tag="OO179_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533838.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            2943..3059
                     /locus_tag="OO179_003960"
     CDS             2943..3059
                     /locus_tag="OO179_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5533839.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3423..4265
                     /locus_tag="OO179_003961"
     CDS             3423..4265
                     /locus_tag="OO179_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5533840.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4268..>4867
                     /gene="capU"
                     /locus_tag="OO179_003962"
     CDS             4268..>4867
                     /gene="capU"
                     /locus_tag="OO179_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5533841.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGH"
BASE COUNT         1298 a         1152 c         1136 g         1281 t
ORIGIN      
        1 tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc
       61 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat
      121 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      181 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg
      241 gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt
      301 gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc
      361 cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc
      421 ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac
      481 cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga
      541 cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg
      601 cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag
      661 caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc
      721 tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat
      781 atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag
      841 taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc
      901 attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt
      961 tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat
     1021 aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc
     1081 ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat
     1141 ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg
     1201 atgctccacc tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata
     1261 cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg
     1321 ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa
     1381 tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt
     1441 aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg
     1501 ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc
     1561 ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct
     1621 tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag
     1681 caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt
     1741 aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga
     1801 gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg
     1861 acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac
     1921 aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact
     1981 gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt
     2041 cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg
     2101 ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag
     2161 ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc
     2221 actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga
     2281 aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc
     2341 tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat
     2401 tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag
     2461 cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg
     2521 gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc
     2581 cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg
     2641 tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg
     2701 gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt
     2761 ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa
     2821 ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc
     2881 tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa
     2941 ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat
     3001 ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc
     3061 tgcaggcctg tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc
     3121 cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag
     3181 gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt
     3241 gagtcgtttt taaaagatat gttaatatat cattgtttat gaattgttga tgtttaattt
     3301 agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata
     3361 aaatgcacca gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt
     3421 aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc
     3481 atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa
     3541 taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt
     3601 atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg
     3661 acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt
     3721 taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc
     3781 atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt
     3841 ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc
     3901 atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg
     3961 acttgttgag cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt
     4021 gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct
     4081 attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag
     4141 gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt
     4201 atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg
     4261 attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca
     4321 ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag
     4381 ggagaacagc aaaattgctt ttgaagccag taagtttgga attgatatca cattcgcgtt
     4441 atttcgaaac agtcttcaca ttcctactgt atggagatta ctcggaatag ttcatagttt
     4501 tcagcccgat gcaatcgttt gtcacagtgg acatgatagt aatattgttg gtttggtacg
     4561 gttttttacc tggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa
     4621 aacaaaattt ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaaatat
     4681 gaagacacat ctggagcagg aaggatgtcg aactcgggtt actgttgtgc ctcctggctt
     4741 tgatttccag gaattatacg ttgattcccg aaacagtttg cctccgagtg ttctttcctg
     4801 gctggcgtcc cgaagaggat gccccgtaat tgctcaggta ggaatgttgc gcccggaaaa
     4861 agggcac
//
LOCUS       DAJTNW010000181         4866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNW010000181 DAJTNW010000000
VERSION     DAJTNW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..969
                     /locus_tag="OO179_003963"
     CDS             <1..969
                     /locus_tag="OO179_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5533842.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="OO179_003964"
     CDS             1550..2773
                     /locus_tag="OO179_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533843.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="OO179_003965"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="OO179_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5533844.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="OO179_003966"
     CDS             3742..4272
                     /locus_tag="OO179_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5533845.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4866)
                     /locus_tag="OO179_003967"
                     /pseudo
     CDS             complement(4314..4866)
                     /locus_tag="OO179_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          998 c          905 g         1407 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aatacatgtg
     4861 tgcgta
//
LOCUS       DAJTNW010000182         4861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNW010000182 DAJTNW010000000
VERSION     DAJTNW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.186"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..425
                     /locus_tag="OO179_003968"
     CDS             <1..425
                     /locus_tag="OO179_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5533846.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="OO179_003969"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="OO179_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5533847.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="OO179_003970"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="OO179_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5533848.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="OO179_003971"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="OO179_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5533849.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4861
                     /locus_tag="OO179_003972"
     CDS             4720..>4861
                     /locus_tag="OO179_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5533850.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1389 a         1037 c         1079 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4861 a
//
LOCUS       DAJTNW010000183         4747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNW010000183 DAJTNW010000000
VERSION     DAJTNW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            8..4102
                     /gene="sepA"
                     /locus_tag="OO179_003973"
     CDS             8..4102
                     /gene="sepA"
                     /locus_tag="OO179_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5533851.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            complement(4271..>4747)
                     /gene="istB"
                     /locus_tag="OO179_003974"
     CDS             complement(4271..>4747)
                     /gene="istB"
                     /locus_tag="OO179_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
                     /protein_id="HCR5533852.1"
                     /translation="TTDWVDAGENVLLFGASGLGKSHLAAAIVDGVVGQGYRARFYSA
                     GELLQELRKARAQLKLNELLLKLDRYRVIVVDDLGYVKRDSAETGVLFELIAHRYERG
                     SLVITSNHPFSMWGSIFVDETMAVAAADRLIHHGYMFELKGESYRKKTAKAVTSVT"
BASE COUNT         1268 a         1113 c         1228 g         1138 t
ORIGIN      
        1 tttctatatg aacaaaatat attatcttaa gtattgccat ataaccaaaa gcctgattgc
       61 tgtctccgaa ctggcccgca gggttacctg caaaagccat cgcagacttt cacgtcgggt
      121 tattcttacg tctgttgcag ctttatcact ttcctcggca tggccagctc tgtcagcaac
      181 ggtcagtgca gagatccctt atcagatatt ccgcgacttt gccgagaata aaggtcagtt
      241 tacgccgggg accacgaata tttccattta cgacaagcag ggcaatctgg tcggtaaact
      301 tgataaagcc ccgatggctg atttcagtag tgccactatt actaccggta gcctgcctcc
      361 gggagaccat acactttact cccctcagta tgtggttacc gccaaacatg tcagcggttc
      421 tgacacgatg agcttcggat acgccaaaaa cacctatact gcggttggta caaataataa
      481 tagcggtctt gatattaaaa cccgccgcct gagtaaactg gtgacagagg tggcgccggc
      541 agaagtgtct gacatcgggg ctgtgagcgg tgcctatcag gccggcggac gctttactga
      601 attctatcgc cttggggggg ggatgcagta cgtcaaagac aaaaatggta atcgcaccca
      661 agtgtatacc aatggcggat ttcttgttgg gggaaccgtc agcgcgctga actcctataa
      721 caacggacag atgattacag ctcagaccgg tgacattttt aatcccgcga atggacctct
      781 ggctaattat ctgaatatgg gggacagcgg ctcccccctg tttgcttatg attccctgca
      841 aaaaaaatgg gtactgattg gcgtgctttc ctcaggaact aactatggaa ataactgggt
      901 cgtcaccact caggatttcc ttggtcagca accgcaaaat gattttgaca aaactatagc
      961 ctacacctct ggtgaaggag tactgcagtg gaaatatgat gcggctaatg gcaccggcac
     1021 actgactcag ggaaacacga cttgggatat gcatggaaag aaaggaaatg atctgaacgc
     1081 aggaaaaaac ctcctgttca ccggcaataa tggcgaggtc gttctgcaga attccgttaa
     1141 tcagggagct gggtatctgc agtttgccgg cgattacagg gtgtccgccc tgaacggcca
     1201 gacatggatg gggggcggga ttatcaccga taaaggaacc cacgttctgt ggcaggtgaa
     1261 cggagttgcc ggtgataacc tgcataaaac tggcgaaggt accctgacag taaacgggac
     1321 cggtgtgaat gcaggcggac tcaaggtggg ggacggtacc gttattctca accagcaggc
     1381 ggatgctgac ggaaaggtac aggctttcag ctctgttggt attgccagcg gtcgtccgac
     1441 agttgtgctt tctgactcac agcaagttaa tccggataat atttcgtggg gataccgtgg
     1501 aggacgtctg gaactgaatg gtaataatct gacctttacc cgtcttcagg cggctgacta
     1561 cggggccatc attaccaata acagtgagaa aaaatcaacg gtaacactcg accttcagac
     1621 attaaaagcc agtgacatca atgtaccggt taatactgtc agtatttttg gggggagagg
     1681 agcgccggga gacctatatt atgacagttc taccaagcaa tatttcatcc tgaaagcgag
     1741 ttcatattcc ccgttcttct ccgatctgaa caacagcagt gtctggcaga atgtcggaaa
     1801 agatcgtaat aaagccattg atactgtgaa gcaacaaaaa attgaggcca gcagccagcc
     1861 ttatatgtat cacggccaac taaacggcaa tatggatgtg aatattccgc agctctcagg
     1921 taaggatgta ctggctcttg atggttcagt taacctgcca gaaggttcga taaccaagaa
     1981 gtccggcacg ctgatattcc agggtcatcc agttattcat gctggaacga cgacttcttc
     2041 cagccagagc gactgggaaa cccgtcagtt tacgctggaa aaactgaaac tcgatgcggc
     2101 aacattccat ctgtccagaa acggcaagat gcagggagat attaacgcca ccaatggaag
     2161 tacagtcatt ctgggaagta gccgtgtctt tactgacagg agtgacggaa ccggtaatgc
     2221 ggtctcctct gttgaaggga gtgccactgc aaccacagtt ggtgaccaga gtgattacag
     2281 cggaaatgtt acgctggaaa ataaatcatc cctgcaaatc atggagagat tcaccggtgg
     2341 tattgaggct tatgatagca ccgtgagtgt gacctctcag aatgctgttt ttgaccgtgt
     2401 tggcagcttt gtcaacagca gcctgaccct cggaaaagga gcaaaactta cggctcagag
     2461 cggtattttc agcaccgggg ctgtggatgt aaaagaaaat gcttctctga ccctgacggg
     2521 gatgccttct gcgcagaaac aggggtatta ctcacccgta atttctacaa cagaagggat
     2581 taacctcgaa gataacgcca gcttttctgt taaaaatatg ggctatctga gttcggatat
     2641 tcatgcagga accactgcgg caaccattaa tctgggagac agtgatgctg atgcagggaa
     2701 gacggactct ccgttattca gctccttaat gaagggatat aacgctgttc tgagaggcag
     2761 tattacgggg gcacagagta cggtaaatat gatcaatgct ctgtggtact ctgacggaaa
     2821 atcagaggcc ggtgcactga aggctaaggg ctcgcgaatt gaactggggg acgggaaaca
     2881 ctttgccact ttacaagtaa aagagctttc tgcagataat accacgttcc tgatgcatac
     2941 caacaacagc cgggctgacc agttgaatgt cacagacaaa ctgtcaggca gtaataatag
     3001 cgtcctggtt gactttttaa acaaaccagc cagtgaaatg agcgtgacgt taattaccgc
     3061 accgaaaggg agtgatgaga aaacgttcac tgccggtacg cagcagattg gtttcagtaa
     3121 cgttacgcca gtaatcagca cggaaaaaac ggatgatgcc acaaaatggg tgctgacagg
     3181 atatcagact accgctgatg ccggagcctc gaaagccgca aaagacttta tggcatcagg
     3241 ttataagtcc ttccttacag aggtcaataa cctgaacaaa cgtatgggtg acctgcggga
     3301 tactcagggg gatgccggtg tctgggcacg cataatgaat ggtaccggtt cggcagatgg
     3361 tgactacagc gataactaca ctcacgttca gattggtgtc gacagaaagc atgagctgga
     3421 cggtgtggat ttatttacgg gggcattgct gacctatacg gacagcaatg caagcagcca
     3481 cgcattcagt ggaaaaaaca aatccgtggg tggcggtctg tatgcctctg cactctttaa
     3541 ttccggagct tattttgacc tgattggtaa atatctccat catgataatc agcacacggc
     3601 gaattttgcc tcactgggaa caaaagacta cagctctcat tcctggtatg ccggtgctga
     3661 agttggttat cgttaccacc tgacgaaaga gtcctgggtg gagccacaga tagagctggt
     3721 ttacggttct gtatcaggaa aagcttttag ctgggaagac cggggaatgg ctctgagcat
     3781 gaaagacaag gattataacc cactgattgg ccgtactggt gttgacgtgg gaagagcctt
     3841 ctccggagac gactggaaaa tcacagctcg agccgggctg ggttatcagt tcgacctgct
     3901 ggcgaacgga gaaacggttc tgcaggatgc ttccggagag aaacgtttcg aaggtgaaaa
     3961 agatagcagg atgctgatga cggtagggat gaatgcggaa attaaggata atatgcgttt
     4021 gggactggag ctggagaaat cagcgttcgg gaaatataat gtggataatg cgataaacgc
     4081 caacttccgt tatgttttct gacgaatggg ataggtctgc cattcggcag gcctttttct
     4141 gaaagaactc cgtgagcctg tttaagattc tgtgtaaatg ccttttctca gaagtgaccg
     4201 tccaggtatt agacgtcaac tagttttgcc gactggcgcc aactatactg gccgcccctt
     4261 cagtgcgaca tcaagtcacg cttgttactg ccttcgctgt ctttttcctg tagctttccc
     4321 ctttcagttc gaacatatat ccgtgatgga tcagtcggtc tgctgccgcc accgccatag
     4381 tctcatccac gaagatgctg ccccacatgc tgaacggatg gttactggtt atcactaggc
     4441 tcccacgttc atagcgatgc gctattaact cgaacagcac acccgtttcg gcgctgtcgc
     4501 gtttgacata gccaagatca tccaccacta tcacccggta gcgatccagt ttcagtagca
     4561 gctcattcag tttcaactga gctctggctt tacgtagttc ctgcaacagc tctcctgcgc
     4621 tgtagaaccg ggcccggtag ccctggccta ctacgccatc cacgatcgct gccgccagat
     4681 ggcttttccc caacccgctg gccccgaaca gcagaacgtt ttctccagca tcaacccagt
     4741 ctgtcgt
//
LOCUS       DAJTNW010000184         4718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNW010000184 DAJTNW010000000
VERSION     DAJTNW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..277)
                     /locus_tag="OO179_003975"
     CDS             complement(<1..277)
                     /locus_tag="OO179_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5533853.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARA"
     gene            complement(282..467)
                     /locus_tag="OO179_003976"
     CDS             complement(282..467)
                     /locus_tag="OO179_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5533854.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(464..3103)
                     /locus_tag="OO179_003977"
     CDS             complement(464..3103)
                     /locus_tag="OO179_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5533855.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3311..4300
                     /gene="ldhA"
                     /locus_tag="OO179_003978"
     CDS             3311..4300
                     /gene="ldhA"
                     /locus_tag="OO179_003978"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5533856.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4411..>4718
                     /gene="hslJ"
                     /locus_tag="OO179_003979"
     CDS             4411..>4718
                     /gene="hslJ"
                     /locus_tag="OO179_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5533857.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1195 a         1229 c         1108 g         1186 t
ORIGIN      
        1 tggcccgagc tacgagcttt tgcccggcga gtttcgcgat atcatctaca gacacattat
       61 tttgctttgc ccgttgttgg taactcgctt tacgttcggc attaatttcg gttactaatt
      121 tctctgtttc agcatccgtt ttcagcgcaa ccagataacc ataaaatgtt tcacctaccc
      181 gcccctgagt tctggcttca tccagagtca atgccattac attgctgctt accagcccaa
      241 caagaaacgc acaaagaagt aatgttttct tcatcatcgc ctcagaaaag atcgctacga
      301 gtttcaagca gctcttcgac atctttgtct gccttgatga tgatctcatg ctcaatttta
      361 acgttcatat tgatagtgat cggttcctta ggtgcagcga cttcaatgcg aggcgtacag
      421 ccaaccagca taaaagatga cgtcaacgca gccagtaaaa ttttcattgt ttttcctcac
      481 actcttttcc ttgcggacag tcatttcccg gcagacgtgc gttctgctcc aaccatgcct
      541 ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa aatattctct tcatgatggt
      601 aattaaggtt taccgtaccg cttttaccat caacgcgact ggtacctgta atgttggcct
      661 gcatggttaa taaaccgaga ttatctaagt taatttttgt cgatgaccgg ctaatttcca
      721 tatagcgcaa ccagttaatt gctgaacccg cagtcatatt gtctttcacc accgcatctg
      781 cggtatcttt atccagccgc aaggtcattg gcccgctatt tgccagccaa ccatctttca
      841 ctatccattt ttcgttgttt agccataacg gcaatgcacc actaaatgcc ccggacatgg
      901 cgaattgttt cggattgacg gcgctaacca gttcgctgga tgagagatta ttcagccgca
      961 acagcgccgg gtcatgttgc ggcatacgta attgtttcat cagcacgtta ccgcctaaca
     1021 catcgacgct aacatcagtc aacagcaagg gttcttcttc agtccacgga taacgccctt
     1081 gcaaatcagc cgtaatattt ttcgctgtca ccagattaat cacttcggca attcgcaacg
     1141 taacggggcc gcgagtcccc agatgccagg ctccatcggc aaaacggaaa ggcaggacaa
     1201 aatcgacacc gttaacctga ttatctggca tccaggcact accgcctttc aacacgccgt
     1261 gccctcccgc gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga gcatacagtt
     1321 caccatcgcg taagttcatc ttccagtcgg gtggcaccag cggctggaat acagtcagtg
     1381 attgttttgg ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga ccatttaccc
     1441 gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa gaggaaatag gtcggattgc
     1501 gcccatcgac gctaaatttt aaggttgatg gcggtaacac actgccgcca gtgaacagcg
     1561 tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg tcacgtaccc
     1621 agacgacggg cttgtcgaga attaatcgcg gcttttctac cgtcatcgta ccgtattgta
     1681 actggtcgaa accggtggag agatcggtca gcgtaatcgt gctgtcatgc cactcacctt
     1741 ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact cccttttccc cagtagcgcc
     1801 actgccagcg gccagcgtca gggagaaaat cattcgccag cccatccata tgcagcacga
     1861 aatcgcccag ttcattttca tgcgcctgca aaatggcctg caaacgtccg tcaacaccac
     1921 gttgggtgac ttttacaccc gctaaaggcc agcggatttc atcgatgtcc agcgaatcga
     1981 tgactcttcc ctttgaacga agtaacgcgc ctggctcaaa ggtcagcgtt gggtcagaca
     2041 gacttccact tagctgcgca ggtaaacggg catataaaat gaggtccgcc tgtttcgctt
     2101 caccggtcag ctgcagaggc agttgactgt tatccatgct taattttcct gggccaaaat
     2161 taagcaccgc gttgccctta cccgcttgcc cctgggtcag cacattcagt cgtccgctga
     2221 ccagagcgtt ctcaagccct gcctgccagt tgtcgacttt gacacccagt cggccactca
     2281 aaggaaaacc tgcatacagc cagctccagc gaccatcgct tacggtcaat tgttgacgag
     2341 taatttgcca cggcaaatcg agcaacggat cgccgttatc ccgtgccagc acgatcaatt
     2401 gcccgctatt ttcctgccag tccagctcgg catccaccag tgacggttcc tgcggcaagt
     2461 ttaacgtcgc agtagcatgc ccacttacag gaagtccatc cggcacgagc ggcatagtaa
     2521 attcccccac cagtttaacc ggcggctgat tttcaaacgc gacgacatcc agttcgctga
     2581 ctgtaagttg ttgccctttc agctggcctt gaaatttaac tttttcgccc tgataacgca
     2641 gttgctggat atcagaggtt aatgcgagag agagttttcc ctgccattcc tgccagggag
     2701 aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc cactgcgcga
     2761 gggtttttgg agcagctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat
     2821 caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg
     2881 tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg attaaacctt
     2941 tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgt
     3001 ccgccagcgt gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa
     3061 tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg
     3121 atttagcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga
     3181 aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc atggctttca
     3241 attatatttg aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg
     3301 agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca
     3361 acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa
     3421 aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag
     3481 ccacccggtg ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc
     3541 cggtttcaat aacgtcgacc ttgatgcggc aaaagaactg gggctgaaag tagtccgtgt
     3601 tccagcctat gatccagagg ccgttgctga acacgccatc ggtatgatga tgacgctgaa
     3661 ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct
     3721 gaccggcttt actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt
     3781 ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc
     3841 aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga
     3901 atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga
     3961 agccgccttc gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt
     4021 gattgattct caggcggcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat
     4081 ggacgtctat gagaacgaac gcgatctgtt ctttgaagat aaatccaacg acgtgatcca
     4141 ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc
     4201 attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa
     4261 tctggaaaaa ggtgaaacct gcccgaacga actggtttaa tcttgcagct cccctgcaat
     4321 ccaggggagc tgattcagat aatccccaat gacctttcat tctctattct taaaatagtc
     4381 ctgagtcaga aactgtaatt gagaaccaca atgaagaaag tagccgcgtt tgttgcgcta
     4441 agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc agaacagcta
     4501 cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag cgataaaaat
     4561 ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt
     4621 agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc aatgacccgt
     4681 atgatgtgcg ctaacccgca gcttaatgaa ctcgataa
//
LOCUS       DAJTNW010000185         4665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNW010000185 DAJTNW010000000
VERSION     DAJTNW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..529
                     /locus_tag="OO179_003980"
     CDS             <1..529
                     /locus_tag="OO179_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5533858.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            726..2165
                     /gene="aldA"
                     /locus_tag="OO179_003981"
     CDS             726..2165
                     /gene="aldA"
                     /locus_tag="OO179_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5533859.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2207..3208)
                     /gene="gap"
                     /locus_tag="OO179_003982"
     CDS             complement(2207..3208)
                     /gene="gap"
                     /locus_tag="OO179_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5533860.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3397..3927
                     /gene="cybB"
                     /locus_tag="OO179_003983"
     CDS             3397..3927
                     /gene="cybB"
                     /locus_tag="OO179_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5533861.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4172..4345
                     /locus_tag="OO179_003984"
     CDS             4172..4345
                     /locus_tag="OO179_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533862.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="OO179_003985"
     CDS             complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="OO179_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5533863.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1190 a         1078 c         1175 g         1222 t
ORIGIN      
        1 ccgcaccact ggcagagcag aagcgaccat cctggtggat atcgctcatc agttctggca
       61 tattccgcat gaaaaaatct ggattgaaga ccagtcaaca aactgcggtg aaaacgcacg
      121 ctttagcatc acgctattga atcaggccgt agaacgagtt catacggcta tcgttgtaca
      181 ggaccccacc atgcagcggc gcacgatggc aacgttccgc cgtatgaccg gggacaatcc
      241 cgatgcacca cgctggttaa gttatcccgg attcgttcct cagttaggaa ataacgcaga
      301 cagtgtaatc tttattaatc agttacaagg attatggccg gttgagcgtt atctctcact
      361 actcactggc gagctgccgc gtttacacga tgatagcgat ggctacggtc cccgcgggcg
      421 agattttatc gttcacgttg attttccggc agaagtcatc catgcatggc aaacgctgaa
      481 acatgatgcg gtgctcatcg aggcgatgga aagtcgctcg ttacgttaaa aattgcccgt
      541 ttgtgaacca cttgtttgca aacgggcatg actcctgact tttatttctg ccttttattc
      601 cttttacact tgtttttatg aagccctcca cagacttgtc ctttcccgat gccgtctctc
      661 tgatgattga tgttaattaa caatatattc accgaaaaca aacatataaa tcacaagagt
      721 cgcccatgtc agtacccgtt caacatccta tgtatatcga tggacagttt gttacctggc
      781 gtggagacgc atggattgat gtggtaaacc ctgctacaga ggctgtcatt tcccgcatac
      841 ccgatggtca ggccgaggat gcccgtaagg caatcgatgc agcagaacgt gcacaaccag
      901 aatgggaagc gttgcctgct attgaacgcg ccagttggtt gtgcaaaatc tccgccggga
      961 tccgcgaacg cgccagtgaa atcagtgcgc tgattgttga agaagggggc aagatccagc
     1021 agctagctga agtcgaagtg gcttttactg ccgactatat cgattacatg gcggagtggg
     1081 cacggcgtta cgagggcgag attattcaaa gcgatcgtcc gggagaaaat attcttctgt
     1141 ttaaacgtgc gcttggtgtg actaccggca ttctgccgtg gaactttccg ttcttcctca
     1201 ttgcccgcaa aatggctccc gctcttttga ccggtaatac catcgtcatt aaacccagtg
     1261 aatttacgcc aaacaatgcg attgcattcg ccaaaatcgt cgatgaaata ggccttccgc
     1321 gcggcgtgtt taaccttgta ctggggcgtg gtgaaaccgt tgggcaagaa ctggcgggta
     1381 acccaaaggt cgcaatggtc agtatgacag gcagcgtctc tgcaggtgag aagatcatgg
     1441 cgactgcggc gaaaaacatc accaaagtgt gtctggaatt gggtggtaaa gcaccggcta
     1501 tcgtaatgga cgatgccgat cttgaactgg cagtcaaagc catcgttgat tcacgcgtca
     1561 ttaatagtgg gcaagtgtgt aactgtgcag aacgtgttta tgtacagaaa ggcatttatg
     1621 atcagttcgt caatctgctg ggtgaagcgt tgcaggcggt tcaatttggt aaccccgctg
     1681 aacgcaacga cattgcgatg gggccgttga ttaacgccgc ggcgctggaa agggttgagc
     1741 aaaaagtggc gcgcgcagta gaagaagggg cgagagtggc gttgggtggc aaagcggtag
     1801 aggggaaagg atattattat ccgccgacat tgctgctgga tgttcgccag gaaatgtcga
     1861 ttatgcatga ggaaaccttt ggcccggtgc tgccagttgt cgcatttgac acgctggaag
     1921 atgctatctc aatggctaat gacagtgatt acggcctgac ctcatcaatc tatacccaaa
     1981 atctgaacgt cgcgatgaaa gccattaaag ggctgaagtt tggtgaaact tacatcaacc
     2041 gtgaaaactt cgaagctatg caaggtttcc acgccggatg gcgtaaatcc ggtattggcg
     2101 gcgcagatgg taaacatggc ctgcatgaat atctgcagac ccaggtggtt tatttacagt
     2161 cttaatgagt gaaagaggcg gaggtttttt cctccgcctg tgcgcgtcag agtttagcga
     2221 atttttcgag ggtgcgaata agctgcgtga cgaagccata ttcgttatcg taccaggcga
     2281 ccgttttcac cagttgtaaa tcgcccacgg cggtaatttc cgtttgcgtg gcatcaaaca
     2341 ctgaaccgaa atggctgcca atgatatcgg aagagactat ttcttcatcg gtataaccaa
     2401 atgactcgtt attggtggtt gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt
     2461 tttttccgag aatggacacc agctcagtga ccgaacctgt tttcaccggc acgcgttgcg
     2521 catgaccttt cagtttgccg ctcagttccg ggatcaccag accaatggct tttgccgccc
     2581 ccgtagtgtg gggaatgata ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac
     2641 gcgggccatc caccagtgac tgggtgccag tataggcatg aatggtcgtc atcgtgccga
     2701 cttctatccc gaaactgtca tgcaaggctt tggccatcgg cgcaaggcag ttagtggtgc
     2761 atgacgccac ggaaacaatg gtgtcgttgc catccagagt gtcgtcatta acgttataaa
     2821 cgatggtttt catttcaccg gcaggagcgg aaatcaacac cttcttcgca ccagcatcaa
     2881 gatgcgcctg cgatttatcg gcggaggtat aaaagccagt acattcgaca atgatttctg
     2941 cccctttcgc tttccacgga atatttttag cctctttttc ggcgtaaacc gcgatacttt
     3001 tcccatcaac gataagtgaa tcttccgtaa aatcaacgct ccaggggaac ggaccatagt
     3061 ttgaatcatg tttcagcaga taggcgagaa tttttgggga agtaagatca ttaatagcga
     3121 caacgtctat gttgcttttg acttcaagta aacgacgcaa caccagtcga ccgatacgac
     3181 caaaaccgtt aataccaact ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa
     3241 attgatcctt tccaggctta gaccaggatt gtgggatggg caatctccat tcttacagtg
     3301 aaacgtaacg tactgaaaac gggtgaacaa tatttaatga aattttgaga aaagcccgtt
     3361 atgttaacgg aaaattatgt taaagcagga aatgttatgg aaaataaata ctcaaggtta
     3421 caaatcagca ttcactggct ggtctttttg ctggttatcg cagcgtattg cgcaatggag
     3481 tttcgtggtt tcttcccacg tagcgatcgg ccgctcatca acatgattca tgtttcctgt
     3541 ggcatctcaa ttctcgtgct gatggtggtt cgtcttttgt taagactgaa atacccaacc
     3601 ccgccgatta tacctaagcc gaaaccgatg atgacgggac tggcgcattt gggacatttg
     3661 gtgatttatc tcctgtttat tgcgctgcca gtgattggtt tggtgatgat gtataaccgg
     3721 ggcaacccgt ggtttgcgtt tggtttgacg atgccttacg cttcagaggc caatttcgaa
     3781 cgggttgata gcttaaagtc gtggcatgag acgctggcga atctgggata ttttgtcatc
     3841 gggttgcacg ctgcggctgc actggcacac cactatttct ggaaggacaa cacacttcta
     3901 cgcatgatgc cgcgtaaacg ttcctgaagg atatttaaag aaaacgcctg tactaaaacc
     3961 gacccgtggt acaggcgaag aatacgggtc tacatcggaa gcgcctatta tatttatttg
     4021 tatgacaaat aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt
     4081 cacccgatct ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg
     4141 cacataagat tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat
     4201 gggaacgctt gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg
     4261 ggggatctca cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc
     4321 atcgaaaaaa acatgcacta actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc
     4381 tagtcagatg cgcggggaaa atccccgcgc ttgcccttac ctggacgtgc aggccatgag
     4441 cgcagcaacc tccttatcac cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag
     4501 gagtgtgaat gccagaatcg taatgcagat aatgagcaga cacaccacca gagggttgcg
     4561 cttcatagcc tttctccttg ccggatggcg ggtaagaggc taacaatctg aattgcttgg
     4621 ttcattcgtt ggcctcggtt tgtactgacc ccaaaaagtt ggaca
//
LOCUS       DAJTNW010000186         4563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNW010000186 DAJTNW010000000
VERSION     DAJTNW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.190"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..238
                     /locus_tag="OO179_003986"
     CDS             <1..238
                     /locus_tag="OO179_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5533864.1"
                     /translation="LFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKK
                     LEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            395..1144
                     /locus_tag="OO179_003987"
     CDS             395..1144
                     /locus_tag="OO179_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5533865.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1166..1732
                     /gene="yieF"
                     /locus_tag="OO179_003988"
                     /gene_synonym="chrR"
     CDS             1166..1732
                     /gene="yieF"
                     /locus_tag="OO179_003988"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5533866.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1786..3123)
                     /gene="adeP"
                     /locus_tag="OO179_003989"
     CDS             complement(1786..3123)
                     /gene="adeP"
                     /locus_tag="OO179_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5533867.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3288..3944
                     /gene="yieH"
                     /locus_tag="OO179_003990"
     CDS             3288..3944
                     /gene="yieH"
                     /locus_tag="OO179_003990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5533868.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4011..4484
                     /locus_tag="OO179_003991"
     CDS             4011..4484
                     /locus_tag="OO179_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5533869.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1222 a         1144 c         1113 g         1084 t
ORIGIN      
        1 actgtttatg gcagcgcaac ccgacaatct gctactggcg accgcgccgc gctactgtca
       61 gtactacaat caactccatc aactgccgtt ggttgctctt cctctcccgt ttgacgaaag
      121 ccagcaaaaa aagctggaag ttccttttac cctgctgtgg cataaacgga acagccataa
      181 tccgaagatc gtctggttac gggaaaccat taaaaacctt tacgcgtcga tggcataacc
      241 gaatcgtatg aaacgcgacc caatttcaca ataaaatgta aaaaagttgt aataagcttg
      301 tctgaatcga acttttagcc gctttagtct gtccatcatt ccagtaaatg attactcttg
      361 tattcataat ggaccattaa gcatggagcg aaaaatggcg actcactttg cccgagggat
      421 tttaacggaa ggacatctga tttctgttcg tctcccctcc cagtgtcatc aagaagcccg
      481 aaacattccc cctcatcgtc aaagccgttt tctggcgtcc agaggtttac tcgcagaact
      541 gatgttcatg ctgtatggca ttggcgaatt gccggaaatc gtcaccctgc cgaaaggtaa
      601 accggttttc agtgataaaa atttgccttc gttttctatt tcctatgccg ggaatatggt
      661 tggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat atggaactac agcgtgcgac
      721 gcgcgggttt catagcccac acgcgcccga taaccacacc ttttccagca atgaatcgct
      781 atggatcagt aaacaaaacg atcctaacga agcgcgggcg cagctcatta cgctgcgccg
      841 aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat ccgcgcgatc tgcagctgct
      901 gcccattgcc ggacgcctga aatgtgctca tgtaaatcat gtagaagcgt tatgcgacgc
      961 ggaggacgtg ctggtgtggt ccgtggcggt cacgcccgcg attgaaaagc tcagtgtctg
     1021 ggagttagat ggcaaacaca gctggaaaag cctgccggat attcacagcc gcgccaacaa
     1081 tcctaccagc cggatgatgc gttttgccca gctctctacc gtgaagtctt tttcgccaaa
     1141 ttgatagaca accacaggag tcattatgtc tgaaaaattg caggtggtta cgttactggg
     1201 gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt accctgccga aaattgctcc
     1261 ggcgagcatg gaagtcaatg cgttaccttc cattgccgac attcccctgt atgacgctga
     1321 cgtacagcag gaagaaggtt ttccagcaac ggttgaagct ctggcggaac agatccgtca
     1381 ggctgacggt gtggtgatcg tcacgccgga atataactac tcggtaccgg gtgggctgaa
     1441 aaatgccatc gactggcttt cccgcctgcc ggatcaaccg ctggccggta aaccggtgtt
     1501 gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc tgtcagtatc acctgcgcca
     1561 gattctggtt ttcctcgatg caatggtgat gaacaagccg gaatttatgg gcggcgtgat
     1621 tcagaacaaa gttgatccgc aaaccggaga agtgattgat cagggtacgc tggaccacct
     1681 gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga gttaagatct aaataaaaaa
     1741 cccgccagta atcatgcatg gcgggttttt aatgcgctat cgattttaat gagcgtcgat
     1801 aaatacaatc ttcaggataa acagcagcgc aacgatgatt acgcacgggc taaggtcacg
     1861 cagacgcccg gtaccaatct tcatcacgca gtaggagata aagcccagcg caataccttc
     1921 ggtaatcgaa aagctgaacg gcatcatcac ggcggtaata aacgccggaa cagattcagt
     1981 aagatcctgc cagttcacgc gagcaagact tgaggtcatc aacacgccaa cgtagatcaa
     2041 cgcgccagct gcagcgtagc ctggcaccat tcccgccagc ggcgacagaa agataaccag
     2101 caggaacagc agaccaacaa ccactgccgt cagaccggta cgaccgccaa ccgatacgcc
     2161 ggaagaggac tcaatataag ccgtaacgga agaagtaccg ataaacgaac cggtcacgga
     2221 agagatactg tcgacataca gcgcctgctt catgcgcggg aatttcccct tctcatccgc
     2281 cagacctgct ttatcggtca cgccaatcag cgtaccggag gagtcaaaca agttgaccaa
     2341 catgaaagag aaaatcaccc ctgccagccc gaggttaaac gacccggcta aatctacatg
     2401 acccacaact gtcattacgc tcggcggcgc agaaacgatg ccattgtagt gcacatcacc
     2461 cagcatccag cccagcagcg tcgtcaccac gatagaaacc agcaccgctg cgtgaatgtt
     2521 gcgcgaggcc agaatagcaa tgatgaaaaa gccgaggata cccagaagta cgctgtgaga
     2581 agtcagatta ccgatgctca tcagcgtttc cgagttagcg acaatcacac ctgcgttttt
     2641 cagccccatc atgccgataa acaggccgat accgctggta atacccacac gcagacttac
     2701 cggaatgtta gcaatcatcc agtaacgaac gcggaaaatg gtcaaaagaa gcagtccgac
     2761 agcgccccag aagattgcgc ccataccgat ctgccacggc agccccattg cctggacaac
     2821 cacaaaagcg aaaaaggcgt taagccccat cgcaggagcc agcgccaccg gaaggtttgc
     2881 aaacaatccc atcataatgc taccgaaagc agcaatcaga caggttgtta cgaagacggc
     2941 actggtgtcc atgccagcaa cgcccagaat ttgtgggtta acaaatacga tgtagaccat
     3001 cgtcaggaag gtggtgaacc cggcaatcac ttcagttcgt gccgtagtac catgttcgcg
     3061 cagtttaaaa acacgttcca gcatcctctg accagaagtc tgggtggtat gttgatgact
     3121 cattatctat ttccgaacaa ggagggaaaa tccgtcgcta tcgtatacca aaatgcgaca
     3181 ataggcgcgt ttgtgagaga cttttttatt ggatttactt atacggcaac gattgcgttg
     3241 cgcaaatcgg ctctacgaaa acgttaaact gattaaaaag gaaaggcatg tcccggatag
     3301 aagcggtatt tttcgactgc gacggtacgc tggtcgacag tgaagtcatt tgctcccgcg
     3361 catatgtaac gatgtttcag gaatttggta ttacgctcga tcctgaagag gtattcaaac
     3421 gtttcaaagg tgtaaaactg tacgaaatta tcgatattgt ttcccttgaa catggtgtta
     3481 cgttagcgaa aacagaagct gaacacgttt accgtgcaga agtcgctcgg ctgttcgatt
     3541 cagccatcga aggggctgga gcgctcctgt cagcgatcac tgcgccaatg tgtgtggtat
     3601 ctaacggccc aaataacaaa atgcagcatt ctatgggcaa gctgaatatg ttgcactact
     3661 tcccggataa actgttcagc ggctacgata ttcagcgctg gaagccagac ccggcgttaa
     3721 tgttccatgc ggcaaaagcg atgaatgtaa atgtagaaaa ctgcattctg gttgatgact
     3781 cagttgccgg tgcgcaatct ggtatcgacg caggtatgga agtgttctac ttctgcgccg
     3841 acccgcacaa taagccgatc gttcacccga aagtcaccac ctttacccat ctttcgcagt
     3901 taccggaact gtggaaagcg cgtggttggg atattacggc atagttcttc acactccctt
     3961 cacttacccc gcttaaattg gcgctcaaag gtaagtaaag ggagtttgat atgtctgttt
     4021 cacgtcgggt aatacatcac ggactttatt ttgcggtttt aggaccgtta attggtgttc
     4081 tgtttcttgt cctctacata ttcttcgcaa aagaaccgct ggttcttttg gtaataatac
     4141 aggtacttcc attgtttctc ttattgtcga ttactacggg agctattcct gcattgttaa
     4201 ccggcgtgat ggtcgcctgt ctgccggaaa agatcgggtc acagaaaaat tatcgttgtc
     4261 tggctggtgg cataggtggc gtcgttatca ccgagatcta ttgtgcagtt attgtacata
     4321 ttaagggcat ggcttcctcg gagttgtttg aaaacattct ttctggtgac agtctcgttg
     4381 tccgcatcat tcctgcattg ctggcaggtg tggtgatgag cagaatcatt acccgtctgc
     4441 ccggattgga tatttcatgt cctgaaacag actctttaag ttaagcggga tactttatct
     4501 ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg ccgttacccc
     4561 aat
//
LOCUS       DAJTNW010000187         4562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNW010000187 DAJTNW010000000
VERSION     DAJTNW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.191"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(237..1139)
                     /locus_tag="OO179_003992"
                     /pseudo
     CDS             complement(237..1139)
                     /locus_tag="OO179_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1199..1477
                     /locus_tag="OO179_003993"
     CDS             1199..1477
                     /locus_tag="OO179_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533870.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1478..2005)
                     /gene="gadE"
                     /locus_tag="OO179_003994"
     CDS             complement(1478..2005)
                     /gene="gadE"
                     /locus_tag="OO179_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5533871.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2804..3376)
                     /gene="hdeD"
                     /locus_tag="OO179_003995"
     CDS             complement(2804..3376)
                     /gene="hdeD"
                     /locus_tag="OO179_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5533872.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3631..3963
                     /gene="hdeA"
                     /locus_tag="OO179_003996"
     CDS             3631..3963
                     /gene="hdeA"
                     /locus_tag="OO179_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5533873.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4079..4405
                     /gene="hdeB"
                     /locus_tag="OO179_003997"
     CDS             4079..4405
                     /gene="hdeB"
                     /locus_tag="OO179_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5533874.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNENDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4469..>4562
                     /locus_tag="OO179_003998"
     CDS             4469..>4562
                     /locus_tag="OO179_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR5533875.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1343 a          969 c          962 g         1288 t
ORIGIN      
        1 cctttagggt gcctttaaaa ttcgttttta aaggctacga tgacattttt gaagagtctt
       61 aaaagaatta aaatacacgg cgcgcctatc ttgaatgttt tatcgatatc gaagcattga
      121 gcatttttaa ctcgttttat cttgaatgca atcggcgcat aacttattga atcccatcaa
      181 atcccgtctt tttttaccca ttcccggtta tcttgaataa attacctagt cataaataca
      241 ttctggcggc taccttcatc cactaatgcc gtgacataca tgccaggcag caagtcacca
      301 tttgggttgg ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc aaccgtcggg
      361 tcggagaatt tcagcgtgcc ggtctggctg taacgtttac cattttccag attgagctgt
      421 actggcgtac tgccctgaac ctgtttgatt tgcccactgg cgacctcttc tttcatgcgt
      481 aagaaatctt gcaccgactg cgtgagatcg acataaatcg ggtccagacg ttgtacggta
      541 accagcgaat ctgcctgatt agcggtaacg agtgcgccga cggtcaccga cgatttcccg
      601 ctgacgcccg taatcggcga ggtgacattc gcgtattgca gattgatcgt cgcctgttca
      661 acagccgctt tggcgacggt gacattggct tctgcttcat tcagctgggt gcgcgcggtg
      721 tcgtaatcct gacggctaac gtagttggtc ttcagcaacg atgcctggcg gttaaaggtg
      781 atgcgggcat tgctggcggt agagagcgct ttcgccagcg agcctttggc ggagtttagc
      841 tcggcctgta aaggtgcagg atcaatctga tacagcgaat cgccctggtt cactttatcg
      901 ccttcgataa agttgcgttt aatgataata ccgcccacct ggggacgtat ctcggcaact
      961 tcataaggaa cggttctacc gggcaattcg ctcaacacat tgaccgaacc aggggagagt
     1021 gtgacgacac cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt gtcatcgcag
     1081 gcggtgagca tcgcgccgca gaataacaac ggtattaaca gctttcttct tctgttcatt
     1141 ttagtccctg aaaattcttg agaaataacg gttctttaaa ttgcgcgcga ggagagatat
     1201 gagggattta cagaccagcg gcatagtggg gttgtcagcc agtaaggtgg gatacaggca
     1261 cagtgatcga catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc caacagttcc
     1321 tgccagcatt cgggcaacga agggttctac tggtggatac acataccagg ggaatcaagt
     1381 cgttttagcc tctcctttag taatcaccgg gggtaatgaa atcagcatct gcatccctcg
     1441 tcatgccagc catcaatttc agttgcttat gtcctgacta aaaataagat gtgataccca
     1501 gggtgacgat gtcgctcata cgtcttagtt tcagcttgta cataatgttg aatttctgaa
     1561 tctttaatgt ttgttgcgaa atattgagcg tcgacgtgat attgctttca tttttatttt
     1621 tcagaatcag ttgcataatt ttgaactgat tatgtgaaaa acataattgt gaattcttat
     1681 ggggcaagtg tttaccataa gtaatctcaa gaagattttt gatactttct ttgcggctaa
     1741 caaaataaat aggcgcttta gcttttagtt tactgatgtg ataaggtgcc aaaacaataa
     1801 ttctctcggc atctaatttc tccagaaatt taatcgcttc ttcatcaagg atatgattgt
     1861 gataggtatc aatgataacc ttgaagggtt tattgcctac ttttttgatc tctgacaggg
     1921 aattgatttt tatcatttcg tgattatctt tcaactgcca aaagccctgt aaaagaaaag
     1981 aatctttcgt catgagaaaa atcataactt gctccttagc cgttatcgtt tcgaacatgt
     2041 catccttttc attatttaca tccttgtccg aatcgttgtt caatatagta aacgcctata
     2101 agaactttgc ccttcgtgaa ctaatacgtc tattaacact aatcaatact tcagatttat
     2161 ccatcacgca ctccatatat tgatcctaag taagattcct ggttgttatc agcttgtaaa
     2221 tagtaaacaa aaagttaacg tttgcggatt actgcccaag aataagttat caataaccag
     2281 tttgtgatct ctgaagaata ttactaaagt taaaatgtaa tccgatttaa atatcgagtc
     2341 tccttgtttc gacttaagct ggcaattgga ttgccagctt tcttttaacg cattgaaatt
     2401 acctgagtaa ataacgcatt catgtcttca ggtttttata tagcgtctgg agacacggat
     2461 atttatgcaa tgtaatcaac gccagttgca aaaaaatgaa cccggattac ttatttaacc
     2521 gcccatagca tcaacgctta cggaactctt tgttcacttt cccacagcac ttttaaaaca
     2581 ttaaacctga ctacggaaaa tatcagccat gataatgttt gaaatggcta atttgccatg
     2641 gagtgaaaaa aattagatga aattcagtag gttgaaataa tcactagcag gtaattattt
     2701 caatgatagt gcgcaattga tctacaacac tgcgtagcgg agagagtatt aatcggatca
     2761 tagtcacatc aagtgactat gatccgggtg acaaccgggg taattattgc tgcttaacga
     2821 acaaactggc gaagctgaac aggctggcgg cgctaaatat cagttcaatt cccaccagtg
     2881 tggaaaccag cgttacagac accatcggcg ttgcaccaag gaatatccag gcaatgacga
     2941 tatccagcac accaataacg agctgtagcc agctgccttt cattgaacgc tgacgatacc
     3001 aactcatcag gcgaataacc cctgcaacac agaacaaacc ggcaataaat gccgcaatgg
     3061 caaaaatgcc cagctccggt gcacggatga agaaatagcc gatcaataaa taggcgactg
     3121 cgacgaggaa accggataat accggccaga aattatgact gcggttgctg aataacccga
     3181 caataagcgc aatacccgag cagattaata atgcacccac tactgtgctt aaaatatcgc
     3241 cagagacgaa cgggaaactg atacacagca acccgacgat aaacagcagc acggcaataa
     3301 actggattgc tctgcgatgc tttttcagca tctccagatc aaacttcaaa attgttgcct
     3361 tatctatata taacatagaa ccaccctata aaattaagaa gaaaatcccc tgctatcaat
     3421 ctatgccaaa aacgcgtcta agaatgcagt cgatttaata aaaatttcct aattgcagta
     3481 tctgatgcat ctgtaactca ttgtattgaa ataaaaatat ctgattttga tattttccat
     3541 caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg
     3601 ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt
     3661 ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc agctgataac
     3721 aaaaaaccgg tcaactcatg gacctgtgaa gatttcctgg ctgtggacga atccttccag
     3781 ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga agatgcggtt
     3841 ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg tactcaggat
     3901 aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg
     3961 taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg gaatatcttc
     4021 tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg gttacaaaat
     4081 gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg ctttgtcact
     4141 ggtgaacgca caatctgcgc tggcagccaa tgaatctgct aaagatatga cctgccagga
     4201 atttattgat ctgaatccaa aagcaatgac cccggttgca tggtggatgc tgcatgaaga
     4261 aacagtatat aaaggtggcg ataccgttac tttaaatgaa aacgatctca ctcaaattcc
     4321 taaagtgatc gaatactgta agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca
     4381 agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc aagttgagcc
     4441 atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac gtcttatact
     4501 cgcggcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg
     4561 gt
//
LOCUS       DAJTNW010000188         4559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNW010000188 DAJTNW010000000
VERSION     DAJTNW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            294..854
                     /locus_tag="OO179_003999"
     CDS             294..854
                     /locus_tag="OO179_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5533876.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            797..1186
                     /locus_tag="OO179_004000"
                     /pseudo
     CDS             797..1186
                     /locus_tag="OO179_004000"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1166..1723
                     /locus_tag="OO179_004001"
                     /pseudo
     CDS             1166..1723
                     /locus_tag="OO179_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1821..3430
                     /locus_tag="OO179_004002"
                     /pseudo
     CDS             1821..3430
                     /locus_tag="OO179_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3531..4022)
                     /locus_tag="OO179_004003"
     CDS             complement(3531..4022)
                     /locus_tag="OO179_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCR5533877.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4027..4203)
                     /locus_tag="OO179_004004"
     CDS             complement(4027..4203)
                     /locus_tag="OO179_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5533878.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1400 a          728 c          901 g         1530 t
ORIGIN      
        1 aatcccaatg cctttttcaa aataaattag gattaaaata attaaatcag taaattccga
       61 tgcatgattt cggatttttc gaaagtcctg atgaaaggct gcgctacggc actcacggat
      121 ttaattgtta ttcctaatgt atctatgaat taatgtttta taaggatttt ttataaccat
      181 ttataagtat tttcaagcct ggcttgttgc aaacaatgta taaagcactt aggcaataat
      241 aattacattc agcaactatc atcatcggta ttgtttgtgg gcggaatttc atattgacaa
      301 cagtacagtt cttatatcta ttaataatag aaagagatct acaacctaca gattggtgta
      361 gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc ttattgtttg
      421 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      481 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      541 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      601 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatta
      661 ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat tatttgtttc
      721 gtcagatgag tttgtgcata ttaatatttc tggcattgat gaataaagtt agtgaaaaca
      781 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg tttttgtttt
      841 tggggggccg atagttgctc acatactttc tagtcattat gagagctaca atttacacat
      901 tgccgagtta accaatgaaa atggtcaggt ggtttggaaa gcctcatatg ttaccataat
      961 gattttcatg tggttaacct tgctttcggt taatctatac tttaatggtt tacgctatga
     1021 tatctggaat ggagtgacgg tgattgcttt ttgtgccgta ctgtacaata tctctctgtt
     1081 atttatgagc aggtatagcg tgtccacttg gtatattagt cgcactattg aagtagtcag
     1141 taagttaacc gttatggtaa tatttatgtg ccatatattc agtgcgctac gagtaacaaa
     1201 gaacattgca catcgcgatc ccttaaccaa tatatttaac agaaattatt tttttaatga
     1261 actgacagtt caatcagcat cagccaaaaa aacgccttat tgtgtcatga ttatggatat
     1321 cgaccacttc aaagaagtca acgacacctg agggcatccg gttggcgatc aggtgataaa
     1381 aacagtggtg aatatcattg ggaaaagcat acgaccagat gaccttttag cgcgcgtcgg
     1441 cggcgaagag tttggcgtct tgctgacgga catcgatact gaacgtgcga aagctttagc
     1501 ggaaaggatt cgggaaaatg ttgagcgttt aactggcgat aatcctgaat acgctatacc
     1561 acaaaaagtg acgattagta ttggcgctgt tgtgactcag gaaaatgcgt taaacccgaa
     1621 tgagatctat cgactggctg ataacgcact atacgaggcg aaagagaccg ggcgtaataa
     1681 ggtggttgtg agggatgtgg tgagtttttg tgagtcacca taaagcggca ttttagcggg
     1741 tgattttggt aagcgctaaa tagctattcg cagacttgcg taacaatttt cagaaatatc
     1801 atagtactat atgatcttaa atgcttatca ttgatcaatc tgctatcacc cgcttttact
     1861 ggcaattgta ctataatttc ttaggttgca tcaccctgat gcgtggggaa acatgtatgg
     1921 tattcaacaa aaaaatgttt gtactcatta taataccagg cattctgggg gtgttactat
     1981 cgtttgctat gtcaatattt caaatgaata gagatacgac aattaccgcc ggtattctgt
     2041 taaaacagct tgataatgtt acgcaaattg tccagcatac gacaaagctt acctctattc
     2101 tggtgatgaa gccctgcaaa gacattcttg aacagttgat tgctaacgga gcattgactc
     2161 cctatgttcg aacaacgggt cttatagaaa ataatttcca aatatgttct tcagttagcg
     2221 gtttcaaaaa aatgaatgtt aacgatgttt acggtacgag ctttcacaat aaaaataaag
     2281 aatcacgtat agtatcaatt agcggtacaa gttttgtgcc aggtaaaaca gcaatagtgt
     2341 tccttatgcc aatagggaat gatatgacag cttttagtat tgtagagtca cgatatatct
     2401 atgatctaat ggatgttctt gatgatgaga atgatgattc attttctctt cgttttactg
     2461 aagggcctgc aattattagc ggtgtaaata ataatgacag gttatatatg ttaaaaagag
     2521 attttaattc tgcgatcagt caggctcgtc ttacagttac tacgccgatg atatcgttat
     2581 atccctatat cattagcaat gtgctttata ttttgccgtt atctattatt ctctctttta
     2641 ttctctattt cttatggcag cactggatgt cacggaaaat gtcgctggca gaagaaatta
     2701 aaaaaggaat gtcatccgga gaattttctg tgcattatca accagtgtgc gacacgacaa
     2761 ccaaagcgtg tctgggggtt gaagcgttaa tgcgctggca acgcgaagat ggtaaaaata
     2821 tatctccggt agtttttatc cgcgctgcag aagaggaaaa tttaataata ccgttaacta
     2881 aacatctctt tgaactgatt attcaggatg tacaaagctg gaaagtgaaa aagccgtttc
     2941 accttgggat aaatattgcc gcggaacatc ttgcacatcc tgactttgtt ggtgatgttt
     3001 tacatattaa aaatgcaata tctgataaat ttaatattgt ccttgagatt accgaacgca
     3061 atctggttga agacactgat cttgcattac agaaaataaa tgaattaagg atccatggct
     3121 gtgaatttgc agtcgatgat tttggcacgg gatattgttc ccttggcctt ctgcaaaagt
     3181 tgtctgttga ttatcttaaa atagataaaa gctttataga cacacttact acagcggaag
     3241 acgaaacgcc agttttagat attattatta agctgagtaa tcggctcaat ttgataacga
     3301 ttgcagaagg tgtcagtact cctcaccagc agaatatctt attcataata acgtgacatt
     3361 agttcaggga tacttatatg aaaaaccaat gaaagcgaca gagttttatc agtggtatgt
     3421 aaacagataa ataattaaaa atattggaat ggttttgtat tgtcttaaaa tataaacaaa
     3481 gcaatcagaa tgttattaac tgatgacaaa cgccaagatc aacaatgctg ttagtgagta
     3541 atacatatga atcgtggtgt cctgcctgta gagaatatgt agttataatc attttgacgc
     3601 gtaacataat aattaattga agtgtctgct tttatgaaga aatcagaagt gatattttca
     3661 aggatttcat cgctgatgtt atcatctttt tgtatttttt ggattttaac tgtggtgaga
     3721 taaacggtat cacggaattt cttgtatgta aaactcacat atcggcggat gaagcccatt
     3781 ttttttccat ctaccgtgta tgcgcccgtg agatacatgt ttcctgtgtt attttcagaa
     3841 atcatattta acgaaaaata ttgatccatt tttgtatgat cggtgaaatt aataacatgg
     3901 gcattacatg atatgttttg ctctgctggt tcattggaat gatgaaacgc aatatggctc
     3961 agaattgctg caataaaagc acataaaatt attcccgaaa acagaaaata tctttgagtc
     4021 atatgattac tccgctttat aatagagaga gttgcaatta tttggtgatt cagagtttaa
     4081 catttgacta caggtgaaag cataaaaccc gctcatgcta tcatttgttg caatgtaaat
     4141 gtaaggatgt attttgcagt tgacttccat tttctgcaat ttttcaataa ttggtgatag
     4201 catgtctacg gaattaaagt aattattatc atagaaataa ctgcatcctt cctgagtagg
     4261 gtgttgagca taatttgaga aagcatctat tttttttata gtgtgagatt tgctttgaaa
     4321 agccatatcg aaaaaataag aagacatata aactatcccc catgccgcaa aaaaaaacta
     4381 taagtaatgt attctttgtg gtaaatatac cttttgcaac gtaattttta tttatatttt
     4441 cagtgtgggt gccattctct tcactctcaa cactttcatt ctcattgtga ttattgtcga
     4501 gaacagttat gttgatttct tcattgattt tgaagccttt ccgtggcacc gttctgata
//
LOCUS       DAJTNW010000189         4545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNW010000189 DAJTNW010000000
VERSION     DAJTNW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1108..2055
                     /gene="icsP"
                     /locus_tag="OO179_004005"
     CDS             1108..2055
                     /gene="icsP"
                     /locus_tag="OO179_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5533879.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2617..2989)
                     /locus_tag="OO179_004006"
                     /pseudo
     CDS             complement(2617..2989)
                     /locus_tag="OO179_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3233..3352
                     /locus_tag="OO179_004007"
                     /pseudo
     CDS             3233..3352
                     /locus_tag="OO179_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3602..4468
                     /gene="ospB"
                     /locus_tag="OO179_004008"
     CDS             3602..4468
                     /gene="ospB"
                     /locus_tag="OO179_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5533880.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
BASE COUNT         1444 a          827 c          904 g         1370 t
ORIGIN      
        1 acaatagctt atccaaactg ccaagcctct ttattataag taagatctgg cactggaata
       61 ttctcaaaag gactaacatc atcaccacga ccttcaacca accattttat tgcatttaaa
      121 tcccagaata gttcagtagt ggtcaatttg ggaaacgcac aatattgtac ggttacgtta
      181 tcgcaataga gtgaactctc gccctatacg gcgttttcaa ggattagtcc ttaatcggac
      241 aaccacatta aagcataata aataattaag ctttaataat atactataga tacagttcta
      301 ttataaaaaa tactatttta ggtgatgcat taaattagta gaatatttat tcttttataa
      361 aagcaccaat gaattgtcca agtaaacatg agataaaatg tgtagaggat aactgagttt
      421 ggtcacttta actgtattag tcgcagtctg actctcgact ttaaaaggat gggaattacc
      481 tggactgata gtgagaaagc tttccattga aataagtcaa ataaaagttg atggcttcag
      541 tgctaaagtg aggtgcccgc tagccgtatt cactcgaatc ggaattcaat gtattgcttc
      601 tgccatttcc ataatatttc acgggaaaaa caaaatctga aaaactatac ttaagtgttg
      661 tattgagtca gttgagatac aaatacatat ttatcagcac acgttacgtc aaatacagga
      721 ggttgtcatg ttggtactga aaggcacgtt ggctattgca gccgtgatga caatttattg
      781 tcttatcgtt gttcttatgg aacgcttttc tgattgattt catattggcg agagaacaag
      841 aaggtgagta gaattgtcgc ggaatcctga aactatcagc ctcaggtatg aacaccaaaa
      901 ggtaggtaga gaaatctatg gcccccgtta ctttattcgc acaatgaggt atttttttat
      961 ttttacaaaa gttcaataaa tattataata aaagatatgt tcttggtaac ataaacaaat
     1021 atttaattaa aataatgctt ggcattgacg tttatcttat aaagtaagaa gatcatggac
     1081 atttcaacca aaaaagtaga gttctcgatg aaattaaaat tctttgtact tgcactttgt
     1141 gtacctgcga tctttactac acatgctacc actaactatc cacttttcat accggacaac
     1201 atcagtacgg atattagtct aggatctctg agtggcaaaa caaaagaacg cgtttatcat
     1261 cccaaggaag gggggagaaa aattagtcaa ctggactgga aatacagtaa tgcgactatt
     1321 gttagaggtg gcatcgattg gaagctaatt ccaaaagtgt ctttcggagt ttccggttgg
     1381 actactttag gtaaccagaa agcaagcatg gttgataaag actggaacaa ttccaatacc
     1441 cctcaggtat ggaccgacca gagctggcat cccaatacgc atctccgtga tgctaacgaa
     1501 ttcgagctga atcttaaagg ttggttatta aataatttgg attatcgact tggactaata
     1561 gcaggttacc aagagagtcg ttacagtttt aatgcaatgg gagggagtta tatttatagt
     1621 gaaaacgggg ggagcaggaa taaaaaaggg gcacatccta gtggtgaaag aacaataggt
     1681 tacaaacagc tcttcaaaat accttatatt ggattaactg ctaattaccg ccatgagaat
     1741 tttgagtttg gagcagaact gaaatatagt ggttgggttc tttcatctga tacagataaa
     1801 cactatcaga ctgagacaat ttttaaagat gaaataaaaa accaaaatta ctgctctgtt
     1861 gctgcgaata ttggatacta tgtcaccccc agtgcaaaat tttatataga aggctccaga
     1921 aattacattt ctaataaaaa aggtgataca tctctttatg agcaaagtac caatatatct
     1981 ggcaccatta aaaatagtgc aagtattgaa tatattggtt ttctcacttc cgcaggtata
     2041 aagtatattt tttgaattgg ttcaccgagt tgattacgtt ttcatggcta aaaattgccg
     2101 cccattgtga gtcatattcg ccctgacttt ccagaaccat acgaactgcc cgttgacgga
     2161 cttcggggga aaaacgtgta tttttagtca ttctgtttac ctctttctca gggcgtttag
     2221 tctccaggat ttccggggcg gttcaaaaaa ctcttcgttg attactgtgg acttactgtt
     2281 ggcattaccg accctgagac cggagaaata agaaccgctt agatcatcgt ggctgttctc
     2341 ggggtatcaa gttacacatg ggcagaggct acccaaggtc tactattatc gcctgaaagc
     2401 gctgatggaa cagtgctatc agggacatgc tgatgaaaat acctaaataa tcagccagca
     2461 ctctatcttt ccaaatccac agcatagcaa agagagcaaa agagcctgta aattcagaaa
     2521 ttaaggaaac gcctcttggt gtacataatc tatcttatgt taaatgaaat ctaatttctt
     2581 gattatatgc tggatcgttc gtgggtggct ttgcccctat atttccagac acctgttatc
     2641 attggcccgc tgccgcagat attcccgtgg cgagcgataa tccagcgcac tatgcggatg
     2701 ccattcgtta taatgctcga atgcctctgc aaggttcttt gctgccgtta acccgtcagg
     2761 tttgggcatg atactgatgt agtcatactt tattgttttc acgaagctct ttgctattct
     2821 gttactctcc ggactccgca ccgccgtgtt cttcggttca acaaagtccc aacatccggg
     2881 tgaactggtg tgtttcatta gctcggtagc atgaaccatt atccgtcagt cacttcactg
     2941 gagacgccgg aagctcgttg ccgaagcggc gttccaccgc tcccagcatg tcgccaacgg
     3001 gagagcatct acggtctgat tgaactgttt attcaaattg tccaccgtct gtcagtacgc
     3061 tctgaacgtc ggctggttaa aacactgctc gctgattttc agagggttca cggtaaaaca
     3121 gcacttttat tccggattgc ggaagcggct ctgaataatc cggatgggct ggttaaagag
     3181 gttgtgtacc atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
     3241 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
     3301 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
     3361 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
     3421 tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
     3481 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
     3541 tatgaaaata taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg
     3601 tatgaattta gatggtgtta gaccatactg tagaatagtc aataaaaaga atgaaagcat
     3661 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
     3721 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     3781 tattatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     3841 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     3901 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     3961 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     4021 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     4081 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     4141 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     4201 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     4261 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     4321 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     4381 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     4441 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     4501 catttaagat gaagttattt tatctttagt agaaaagtgc ttccc
//
LOCUS       DAJTNW010000190         4538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNW010000190 DAJTNW010000000
VERSION     DAJTNW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..1222
                     /locus_tag="OO179_004009"
     CDS             <1..1222
                     /locus_tag="OO179_004009"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5533881.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1896
                     /locus_tag="OO179_004010"
     CDS             1222..1896
                     /locus_tag="OO179_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5533882.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2424..3185
                     /locus_tag="OO179_004011"
     CDS             2424..3185
                     /locus_tag="OO179_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5533883.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3229..4308
                     /locus_tag="OO179_004012"
     CDS             3229..4308
                     /locus_tag="OO179_004012"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5533884.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4305..>4538
                     /locus_tag="OO179_004013"
     CDS             4305..>4538
                     /locus_tag="OO179_004013"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5533885.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDF"
BASE COUNT         1246 a          862 c         1117 g         1313 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgtagggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttaca aatataggtg atttttttat
     2221 tgtaggcttt tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat
     2281 aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga
     2341 gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag
     2401 ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt
     2461 gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc
     2521 aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt
     2581 cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgcagagta
     2641 aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca
     2701 ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag
     2761 acagggaaaa attacactgg ctatgcgcca ccggcattac gcctaaaaag agtgatgttt
     2821 gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg
     2881 tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg
     2941 cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc
     3001 cgttctacat caacctttct tctttacagg ttggcggtag caagattgag aaactagatt
     3061 atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta
     3121 aatggtctgt gattactgat gaaggtggtg agagtcgttt gtttatatct catttgcaat
     3181 cataatgtat tacattttta ttgaatattt ttatgccatg catattccat gttttatcga
     3241 ctgtcattaa ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca
     3301 tttgattctt cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag
     3361 ggagagcaac tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat
     3421 gttggggagt ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt
     3481 ttaaccgttg atgaccttat ccactatggc attaaaattg ataaaagcag cagtgatact
     3541 gacaataaaa agaatcagtg ttttaaatgg aactctattg aaggtttgaa agtaaattat
     3601 gattttgata gccagcgcgt acagataacc gttccacaac tgtatctgca agataaaaaa
     3661 agtagtttag caccagtgtc gttatggaat gagggtgtag ctgcattccg catggtgtat
     3721 cagacaaata ttgatatttc caaacaaaat gataatcaat ctaccacgcg taattctcgt
     3781 tatggcaggt tcactccagg gtttaatctt ggtgcctgga ggtttcgtag ttcagtgaca
     3841 tggagcaagg aacttggcca gtcagaaaga tggcaaaggg gttacatgtg gtttgagcga
     3901 ggcataaatg cgattaaaag ccgattaacg cttggagaat cttatacgtc gtctgaggtg
     3961 tttgacagca taccttttcg cggcgggatg ttagcgaccg acgatgcgat gactccgccg
     4021 gaggacagct actacacacc tgtggtgcat ggaatagcac aaagtgaggc gcaggttatt
     4081 attaaacaaa atgggcaaat tatttttacc cgttctgtgc cgccagggcc ttttgcgctg
     4141 gataatttac caacgctggc cgttggcggg gagcttgatg ttacagtgag agagagtaat
     4201 ggcgaggagc agtatttttc cgtgccattt caaacgccgg ccatcgctct gcatgaagga
     4261 tatttcaaat atagtgtgat gggggggaat ataaagaaga aggtataaaa agctcccctg
     4321 tctccgtggg cgaaatcacg gcgatgtacg ggcttcctta tggcgtcacg gtgtatggag
     4381 ggattcaaaa cgccactcat tttaatgcaa tatctacggg tattggcatt tctctggggc
     4441 tgttagggag tttatcgaca gatattacgc gttcgattgc gaatttatat tatgggaata
     4501 aatatcgtat tcgttatagt aaatccatta gtgatttt
//
LOCUS       DAJTNW010000191         4429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNW010000191 DAJTNW010000000
VERSION     DAJTNW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO179_004014"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO179_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5533886.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO179_004015"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO179_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5533887.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO179_004016"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO179_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5533888.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO179_004017"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO179_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5533889.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4189..>4429)
                     /locus_tag="OO179_004018"
     CDS             complement(4189..>4429)
                     /locus_tag="OO179_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533890.1"
                     /translation="VSSLGTRWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIA
                     SQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1198 a         1078 c         1061 g         1092 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atatcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa cgcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaacgctgta taaggggcaa
     3961 aaaaggggca aaaaaggggc aaaaaagagt gggttatcgt agcttattgt tgtcgctgat
     4021 aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt gtgatttttg
     4081 cccttacttg ttcaggtcgt attgttcttt ctttgtaatt tgttgatttt cttgcattat
     4141 ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact acgataaccg
     4201 cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac cgtaaatttc
     4261 atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg ggttcgctcc
     4321 tgccgtcaac agccagcagg caaaagtatg tcgcgtgtgg tacggattac ggcggcgaat
     4381 accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttacc
//
LOCUS       DAJTNW010000192         4421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNW010000192 DAJTNW010000000
VERSION     DAJTNW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            226..2649
                     /gene="pgaA"
                     /locus_tag="OO179_004019"
                     /pseudo
     CDS             226..2649
                     /gene="pgaA"
                     /locus_tag="OO179_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2658..4151
                     /gene="pgaB"
                     /locus_tag="OO179_004020"
     CDS             2658..4151
                     /gene="pgaB"
                     /locus_tag="OO179_004020"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR5533891.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4155..4421
                     /gene="pgaB"
                     /locus_tag="OO179_004021"
                     /pseudo
     CDS             4155..4421
                     /gene="pgaB"
                     /locus_tag="OO179_004021"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1247 a          991 c         1032 g         1151 t
ORIGIN      
        1 gatttatgcc gtattcctga agatcctcat cattggaatg gattttcggg cgagaaaagg
       61 attttatatg gacactctgc tcatcatttc ttcttctcat catcaacaat tcacgtctct
      121 cttccgcgtt taataacgga ttatgaggtg caaaaatatc tttcttttca gttacctgta
      181 attagataca gagagagatt ttggcaatac atggagtaat acaggatgta ttcaagtagc
      241 agaaaaaggt gcccgaaaac caaatgggct ttgaaacttc ttactgccgc atttttagca
      301 gcgagtcccg cggcgaagag tgctgttaat aacgcctatg atgcattgat tattgaagct
      361 cgcaagggta atactcagcc agctttgtca tggtttgcac taaaatcagc actcagcaat
      421 aaccaaattg ctgactggtt acagattgcc ttatgggccg ggcaagataa acaggttatt
      481 accgtttaca accgctaccg tcatcagcaa ttaccagcgc gtggttatgc agctgtcgcc
      541 gtcgcttatc gtaacctgca acaatggcaa aactcgctta cactgtggca aaaggcgctc
      601 tctctggagc cgcaaaataa ggattatcaa cggggacaaa ttttaaccct ggcagatgcg
      661 ggtcactatg atactgcgct ggttaaactt aagcagctta actctggagc accggacaaa
      721 gccaatttac tcacagaagc ctatatctat aaactggcgg ggcgtcatca ggatgaatta
      781 cgggcgatga cagagtcatt acctgaaaat gcatctacgc aacaatatcc cacagaatac
      841 gtgcaggcat tacgtaataa tcaacttgct gccgcgattg acgatgccaa tttaacgcca
      901 gatattcgcg ctgatattca tgccgaactg gtcagactgt cgtttatgcc tacgcgcagt
      961 gaaagtgaac gttatgccat tgccaatcgc gccctcgccc aatacgctgc attagaaatt
     1021 ctgtggcacg ataacccaga ccgcactgcc cagtaccagc gtattcaggt tgatcatctt
     1081 ggcgcgttat taactcgcga tcgttataaa gacgtcattt ctcactatca gcgattaaaa
     1141 aagacggggc aaattattcc gccctagggg caatattggg ttgcatcggc ttatctcaaa
     1201 gatcatcagc cgaaaaaagc acagtcaata atgaccgagc tcttttatca caaggagacc
     1261 attgccccgg atttatccga tgaagaactt gcggatctct tttacagcca cctagagagt
     1321 gaaaattatc cgggcgcgct aactgtcacc caacatacca ttaatacttc gccgcctttc
     1381 cttcggttaa tgggcacgcc tacgagcatc ccgaatgata cctagttaca ggggcattcg
     1441 tttctctcaa ccgtagcaaa atatagtaat gatcttcctc aggctgaaat gacagccaga
     1501 gagcttgctt ataacgcacc aggaaatcag ggactgcgca ttgattacgc gagtgtgtta
     1561 caagcccgcg gttggcctcg tgcagcagaa aatgaattaa aaaaagcaga agtgatcgag
     1621 ccacgtaata ttaatctgga ggttgaacaa gcctagacag cattaacgtt acaagaatgg
     1681 cagcaggcag ctgtcttaac gcacgatgtt gtcgaacgtg aaccgcaaga tcccggcgtt
     1741 gtacgtttaa aacgtgcggt tgatgtacat aatcttgcag agcttcgtat agctggttca
     1801 acaggaattg atgccgaagg cccggatagt ggtaaacatg atgtcgactt aaccaccatc
     1861 gtttattcac cactgctgaa ggataactgg cgcggttttg ctggattcgg ttatgccgat
     1921 ggacaattta gcgaaggaaa agggattgtt cgcgactggc tggcgggtgt tgagtggcgg
     1981 tcacgtaata tctggctcga ggcggagtac gctgaacact ttttcaatca tgagcataaa
     2041 cccggcgcgc gcctgtctgg ctggtatgat tttaatgata actggcgtat tggttcgcaa
     2101 ctggaacgcc tctctcaccg cgttccatta cgggcaatga aaaatggtgt tacaggcaac
     2161 agtgctcagg cttatgttcg ctggtatcaa aatgagcggc gtaagtacgg tgtctcctgg
     2221 gctttcactg atttttccga cagtaaccag cgtcatgaag tctcaattga gggtcaggaa
     2281 cgcatctggt cttcaccata tttgattgtc gatttcctac ccagtctgta ttacgaacaa
     2341 aatacagaac acgatacccc atactacaac cctataaaaa cgttcgatat tgttccggca
     2401 tttgaggcaa gtcatttgtt atggcgaagc tatgaaaata gctgggagca aatcttcagc
     2461 gcaggtgttg gtgcctcctg gcaaaaacat tatggcacgg atgtcgtcac ccaactcggc
     2521 tacgggcaac gcattagctg gaatgacgtg attgatgctg gcgcaacgct acgctgggaa
     2581 aaacgacctt atgacggtga cagagaacac aacttatacg ttgaattcga tatgacattc
     2641 agattttaag gataaatatg ttacgtaatg gaaataaata tctcctgatg ctggtgagta
     2701 taattatgct caccgcgtgc atcagccagt caagaacatc atttataccg ccacaggatc
     2761 gcgaatcttt actcgccgag caaccgtggc cgcataatgg ttttgtggcg atttcatggc
     2821 ataacgttga agacgaagct gccgaccagc gttttatgtc agtgcggaca tcagcactgc
     2881 gtgaacaatt tgcctggctg cgcgagaacg gttatcaacc ggtcagtatt gctcaaattc
     2941 gtgaagcaca tcgaggagga aaaccgctac cggaaaaagc agtagtgctg acttttgatg
     3001 acggctacca gagtttttat acccgcgtct tcccaattct tcaggccttc cagtggcctg
     3061 ctgtatgggc ccccgtcggc agttgggtcg atacgccagt ggatgaacaa gtaaaatttg
     3121 gcgatgagat ggtcgatcga gaatattttg ccacgtggca acaagtgcga gaagttgcgc
     3181 gttcccggct cgttgaggtc gcttctcata catggaattc tcactacggt attcaggcta
     3241 attccaccgg cagcttaatg cctgtatatg taaatcgtgc atattttact gaccacgcac
     3301 ggtatgaaac cgcagcagaa taccgggaaa gaattcgtct ggatgctgta aaaatgacgg
     3361 aatacctgcg tacaaaggct gaggtaaatc cacacgtttt tgtttggcct tatggcgaag
     3421 cgaatggcat agcgatagag gaattaaaaa aactcggtta tgacatgttt ttcacccttg
     3481 aatcaggttt ggcaaatgcg tcacaattgg attccattcc gcgagtatta atcgccaata
     3541 atccctcatt aaaagagttt gcccagaaaa ttattaccgt acaggaaaaa tcaccacaac
     3601 ggataatgca tatcgatctt gattacgttt atgacgaaaa ccgccagcaa atggatcgca
     3661 atattgatgt gctaattcag cgggtgaaag atatgcaaat atcaaccgtg tatttgcagg
     3721 catttgctga tcccgatggt gatgggctgg tcaaagaggt ctggtttcca aatcatttgc
     3781 taccaatgaa agcagatatt tttagtcggg ttgcctggca attacgtacc cgctcaggtg
     3841 taaacatcta tgcgtggatg ccggtattaa gctgggattt agatcccaca ttaacgcgag
     3901 taaaatactt accaacaggg gagaaaaaag cacaaattca tcctgaacaa tatcaccgtc
     3961 tctctccttt cgatgacaga gtcagagcac aagttggcat gttatatgaa gatcttgccg
     4021 gacatgctgc ttttgatggc atattgttcc acgatgatgc tttgctttca gattatgaag
     4081 atgccagtgc accggctatc acggcttatc agcaagcagg ctttagcggg agtctgagcg
     4141 aaattcgata aaacccggag caatttaaac agtgggcccg ctttaaaagt cgtgcgttaa
     4201 ctgacttcac tttagaactt agtgcgcgcg taaaagccat tcgcggtcca catattaaaa
     4261 ctgcacgaaa tatttttgca cttccggtaa tacaacctga aagtgaagcc tggtttgcac
     4321 agaattatgc tgatttccta aaaagctatg actggaccgc tattatggct atgccttata
     4381 tggaaggtgt cgcagaaaaa tcggctgacc aatggttaat a
//
LOCUS       DAJTNW010000193         4334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNW010000193 DAJTNW010000000
VERSION     DAJTNW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(130..333)
                     /locus_tag="OO179_004022"
     CDS             complement(130..333)
                     /locus_tag="OO179_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533892.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(335..490)
                     /locus_tag="OO179_004023"
     CDS             complement(335..490)
                     /locus_tag="OO179_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5533893.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(693..1100)
                     /locus_tag="OO179_004024"
     CDS             complement(693..1100)
                     /locus_tag="OO179_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5533894.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1177..1404
                     /locus_tag="OO179_004025"
     CDS             1177..1404
                     /locus_tag="OO179_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5533895.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1388..1810
                     /locus_tag="OO179_004026"
     CDS             1388..1810
                     /locus_tag="OO179_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533896.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1888..2665
                     /locus_tag="OO179_004027"
                     /pseudo
     CDS             1888..2665
                     /locus_tag="OO179_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2672..3418
                     /locus_tag="OO179_004028"
                     /pseudo
     CDS             2672..3418
                     /locus_tag="OO179_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3433..3855
                     /locus_tag="OO179_004029"
     CDS             3433..3855
                     /locus_tag="OO179_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5533897.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3913..4269
                     /locus_tag="OO179_004030"
     CDS             3913..4269
                     /locus_tag="OO179_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533898.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT         1141 a          932 c         1180 g         1081 t
ORIGIN      
        1 ggaacttatg tttccgaaaa gggcggtgat atttatcaaa ttcgcagcca gatatctgac
       61 tatgcagacc gactatccgg gtatcgcgcg tctgtttttg tttgcgaatt cagtcaatgc
      121 gcatggtatt tataccccgc caagacccgg tgaatatcct gcacggcctt ctgctttgta
      181 gaaggcttct tcgctatcat aatcgccaga ttccagcgct tctttagcca gccagatgcg
      241 tgccccagtg cctgcatttg gctccagttg ctggaggcgt tttgcatctt ctaggagtag
      301 agcgataacg tgttttaatt ctgtctcgtt cattttactc acctgaatgt cttcccaacc
      361 aacgacgtgc gccagcttcg gttttaaacg ttttgctttt ggtatacgtt atggcggtga
      421 atgtgccgtc ctggttgggg aacacgccgt acaccggaga ttcgttgttg ccaagatcga
      481 tagtattcat gttgacccca tttcccctta acgccggggt agcggaactg tttgctgaga
      541 acaccgtgcg gtgtcttgat gaataaaatt tagaataacc taaggagggt gatcaagatt
      601 tttatgtaga aaaacctaag ttttttgatg taaaaaacac aagtgtttga aagtttgtgc
      661 tttttattac agggtgtgga gaaaaaaggg gattatttgt ttgcgcttct tttgcgagct
      721 ttgagtagtt cttcaaaaag tttgttgaaa ttttcaactc gagcacgcat ctctgacaac
      781 agggcctttt gctctgactc aggcagtgcg tcgaacagtt gaagtaactc tttttgatct
      841 tctgtcagaa tggctggctg attatccggg atcggttcgc ctggttgttt atcttcatcc
      901 ccaaaaagaa gccaagtcgg tgagcactga agcgcttggc tcagtgcgaa taatcttttc
      961 cccgccggct gtgtttcatc tctttcccat tgagaaattg ttacgtgagc cactttgacc
     1021 agcttaccta atgcggcctg agacagtttt aattttttac gcctatgtaa gaggcgagca
     1081 ccgaaggttt cgtttttcat attagggaat tctaattttt cttgacttag gtttctctac
     1141 ggcctggttt ccttaggaaa atctaaggag ctcgatatgt tgaaaattga tgctatagcg
     1201 ttttttggca gcaaaacaaa gcttgccaat gccgcaggag ttaggttggc aagcattgct
     1261 gcatgggggg aactggttcc tgaaggtcgc gcgatgcgcc tgcaagaggc atctggcggg
     1321 gaacttcagt acgaccccaa agtttatgac gaatatcgta aggcaaaacg acctgggaag
     1381 gtgattcatg aaaatcaggc atgagcacat cgaatcagtg ctgttagcct tggcagccga
     1441 aaaagggcag gcgtgggtcg ctaacgcaat tactgaagaa tatctgcggc aggggggcgg
     1501 cgaattgtct ctggtaccag gcaaggactg gaacaatcag cagaatatct atcaccgttg
     1561 gttgaaaggt gaaacgaaag cgcaaaggga aaaaattcag aaactgatcc ctgcggttct
     1621 ggcaattctt ccgcgtgagc tgcgtcaccg actctgtatc ttcgataccc tggaacgccg
     1681 tgcattactg gcggcgcagg aagcgttgag tacggcaatt gatgcgcatg atgatgctgt
     1741 ccaggccgtt taccggaaag cacatttcag cggtggtggg tcgcccggcg attctgtcgt
     1801 agtgcattga ttgaaattaa tcgtgccgga ttgttttgtt cggtatcagt taaatgtaac
     1861 gctgcgagcg ttacaaggtg aaaacaaatg gcttcaaact ggataaagct cgaggttatt
     1921 acgccggata agccggaaat attcaggctt gctgagattc tgaatattga tccagatgcc
     1981 gcattaggga aggttattcg cttctgggca tgggcggatc aacaaatgat agacggtaat
     2041 gcagattgta acgctcgcgg cgttacaaaa agtgcaatag atcgcatcac ttttatgtct
     2101 ggttttgctg atgcgttaat tcaggttgga tggctggtcg aaaatgacgg tgggctttct
     2161 ctacctaact ttgaacgtca taacggaaaa agctctaaaa aacgggcggt tacaaacgag
     2221 cgagtaacaa aaatacgcga actgaaacga aaaggtaacg ctgccagcgt tacacaaacg
     2281 gatcaaaaag cgttaccagt ggaagaggaa gaggaagatc taaatactga tctcccccta
     2341 aatccccctc gccaaaaacg agcgtctaaa aaattcgagc cggaggctat tgagctgccc
     2401 gattggttgc cggaaacact ctggcatgag tgggtccggt tcagacaggc attgcgaaaa
     2461 ccgattcgaa cggagcaggg cgatacggga actggaaaaa ttccgtcagc agggttttac
     2521 acctgagcag gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa
     2581 aggtgttcgg cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc
     2641 gatcccgcca ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct
     2701 ggaacgcatc cgccgactga ccccgcaaca tgtaaccgcc ccattcagaa cggtagcgga
     2761 gtggcgcgag tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa
     2821 tcgccagttg cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa
     2881 atgctcgttt gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc
     2941 gaaatccatc gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc
     3001 tgataccggg tagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca
     3061 gacagtgatt gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga
     3121 tgggcagtca ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga
     3181 tgaaattggc attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga
     3241 tcgccggaca gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat
     3301 gaaaacattg ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt
     3361 taattttaac tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt
     3421 tcaggaggat ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga
     3481 attgtggaac tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc cgatgaaaca
     3541 ggtatcagtc gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta
     3601 tataattctg gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc
     3661 cgcaaaaaac tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc
     3721 tggtcattac ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc
     3781 gagtgccgga agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag
     3841 gaggcgatac tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca
     3901 aaggagagga gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg
     3961 ataaaagagg atgaccctcg taatcaggtt aattttcttt ataagccatc ggaaagacca
     4021 tattgccatc acgcctctgt acgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc
     4081 tgcggtgcgg ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga
     4141 ctggcagatg atgtaaggct attgcgccag gaggaacagg aaaggcggag aaatatagaa
     4201 aagctgatac agattgagcg taacgcgaaa gcgcggatac gcagggcgac aaaatccaga
     4261 actgaataat taaatttagc actgttaaaa atttaatcct taaccggagg gatttctgca
     4321 ccctcaaatc atca
//
LOCUS       DAJTNW010000194         4245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNW010000194 DAJTNW010000000
VERSION     DAJTNW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            32..913
                     /gene="yddG"
                     /locus_tag="OO179_004031"
     CDS             32..913
                     /gene="yddG"
                     /locus_tag="OO179_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5533899.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1168..2268
                     /gene="ompC"
                     /locus_tag="OO179_004032"
     CDS             1168..2268
                     /gene="ompC"
                     /locus_tag="OO179_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5533900.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2432..2917
                     /locus_tag="OO179_004033"
     CDS             2432..2917
                     /locus_tag="OO179_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5533901.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3027..4016
                     /locus_tag="OO179_004034"
     CDS             3027..4016
                     /locus_tag="OO179_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5533902.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1316 a          875 c          805 g         1249 t
ORIGIN      
        1 ttttaacggc aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg
       61 gctaatagcg atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg
      121 gctcggcccg gtcggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt
      181 cacggttgga tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct
      241 gttattcgtc agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca
      301 tcaggcgatt gaagtgggta tggtgaacta tctgtggccc agcctgacaa ttctctttgc
      361 cattctgttt aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct
      421 ggtcggcgtc tgctgggtgt taggcggtga gaatgggtta cattatgatg aaatcatcaa
      481 taatatcacc accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc
      541 agcctattgc acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt
      601 cctgctaacg ggagcatgtc tgtggattta ctattttctc acgccacaac cagaaatggt
      661 atttagcacg cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta
      721 tactgcatgg aatgtcggta tattgcatgg caatgtcacc ataatggcgg taggctcgta
      781 ttttacgcct gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt
      841 ctcgttctgg caaggcgcac taatggtctg cggcggttcc ctgctctgct ggctggcgac
      901 acgtcgtggt taaaataatt gccgggtcta cggcccggca gttaatcaga taaattccga
      961 caatattaaa aaataaattt caaataccag taattcacct tatatctcat ttcgacttcg
     1021 atcacatatc gataaataat attattctat ttttctcatt gtttttaatc accattctat
     1081 tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa attcgtttag aaaatacgtt
     1141 taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact
     1201 ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga tggtaataaa
     1261 ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa gcgtgacgat
     1321 ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg
     1381 attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc caacggctcg
     1441 cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt tggctctctg
     1501 gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga tatgttgcca
     1561 gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg taccaccggt
     1621 ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa ctttgccgcg
     1681 caatatcaag gcaaaaatga acgtactgac aacagtcatc tttatggtgc tgactacacg
     1741 cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg ctttggtatc
     1801 ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc agctaatcct
     1861 ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta tgatgccaac
     1921 aacatctact ttgcagctaa ttacgatgaa acattaaaca tgaccaccta tggggatggt
     1981 tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt cgacttcggc
     2041 ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg ctacgccgac
     2101 caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa aaatatgtcg
     2161 acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg tgccgtagat
     2221 attcgcaccg ataacatcgt cgctacgggc attacctatc agttctaacg tcctctcctg
     2281 accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc cttaagtaaa
     2341 aatgagcaat tcttgatatt tctggtcatt cacctcaatt aatattattc catatcctcc
     2401 ggggacatct tactataata tttattgatg agtgattatg accaatatta atacagcttg
     2461 tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag aaaccatctc
     2521 cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata atggtgaaga
     2581 aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg catctcttga
     2641 tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta atacgttaaa
     2701 tctggaaaac aattgtctaa cttgtcttga cttagctgac aatgccagtc tcgttaatat
     2761 caacctcagc gttaataaaa ttaacacgat aacctttcca aaagaaccaa aactggaaaa
     2821 catttatatt gatcataaca atctggaaag tttagattta aaaaatcagc attcattggt
     2881 taatctggaa gcgcaaaata atagcctaaa aaaatgatat ttctgatagt tataaactga
     2941 aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg caagaatctc
     3001 tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca
     3061 ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta
     3121 caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc
     3181 aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac
     3241 ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga
     3301 ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacct
     3361 ctatcaatct tgataataac caactcacac attttgatgc aaccaactac gatagactcg
     3421 taaagctaag tctgaatagt aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg
     3481 taagtattac acatatatct atgaataata atgctctcag aaatattgat atagataggc
     3541 tttcatcagt tacttatttt agtgcggcac ataatcaact agagtttgtg caattagaat
     3601 cttgcgaatg gctgcaatac ctgaatctca gccataatca attaactgat attgttgcag
     3661 gtaataaaaa tgaactctta ctgctggatc tatcccataa taaactaaca agtttacaca
     3721 atgtcttatt tcccaacttg aatacgttac ttattaacaa caatttgctt tctgaaatta
     3781 aaatattcta tagcaacttc cgcaatgttc agacattaaa cgctgctaac aaccagttga
     3841 aatatataaa tcttgatttc ctgacttatc ttccatctat caaaagttta agactggaca
     3901 ataataaaat aacccacatt gatactaata atacatccga tattggaact ttattcccca
     3961 taataaaaca gagcaaaaac ttaaattttt taaatgtttc tgggaagaac aattgaccta
     4021 ctatgcagct catgttattt aatttgtttt ccccagcact taagcttaat actggcctgg
     4081 caattctttc gcctggtgca tttgaagttc actctgacgg aatagatgcg gataacgaat
     4141 tgtttcacta tactattaaa aaagcatata ccccatataa tatacacact tacaagacag
     4201 aggaagttgt aaaccagagg aatataaaag ttaaaaacat gacct
//
LOCUS       DAJTNW010000195         4203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNW010000195 DAJTNW010000000
VERSION     DAJTNW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..782
                     /gene="lsrF"
                     /locus_tag="OO179_004035"
     CDS             <1..782
                     /gene="lsrF"
                     /locus_tag="OO179_004035"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5533903.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            806..1096
                     /gene="lsrG"
                     /locus_tag="OO179_004036"
     CDS             806..1096
                     /gene="lsrG"
                     /locus_tag="OO179_004036"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5533904.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="OO179_004037"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="OO179_004037"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5533905.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="OO179_004038"
     CDS             complement(1897..2811)
                     /locus_tag="OO179_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5533906.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            3012..3200
                     /locus_tag="OO179_004039"
     CDS             3012..3200
                     /locus_tag="OO179_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533907.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            3210..4203
                     /locus_tag="OO179_004040"
                     /pseudo
     CDS             3210..4203
                     /locus_tag="OO179_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1053 a         1020 c         1167 g          963 t
ORIGIN      
        1 tggattgggg aatgcagtca cgcttatcgt ggatatttaa tccgaaaacg ggtaaaactg
       61 tgatgctggc ctttgaccat ggttattttc aggggccgac taccggactt gaacgcattg
      121 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
      181 tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg tcaggtgcga
      241 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc
      301 gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
      361 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
      421 ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc
      481 gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg
      541 aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa aaattaccgg
      601 agcgcgagac gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
      661 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
      721 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
      781 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
      841 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
      901 agaaggtaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
      961 ttacgaagcc tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa
     1021 aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa
     1081 tgggttgatg ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagctcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gttcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc tagaaccacg gcagcctgac ggctgcaaat tgaaaactgc ccctgttcgt
     2941 cctgttgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt ttattaagga
     3001 ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct gttggtgctg
     3061 agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg gacccaccac
     3121 ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc cttacccggc
     3181 tacaggatac cgggggttaa tccgtcctca tgttgtccgc ttttactccc cgcccgctga
     3241 aacgtctgtt cacggccaac cagtgctgga catccttcct ggatgcgggc ggtctgcgcg
     3301 atatcgaggt tgaggctgtc acaaaaatgc tggcctgcgg cacgcggata ctgggcgtga
     3361 aggaatacat ctgcgataaa cctgagtgcc cccacgtcag atacgtcact aactcatgcg
     3421 gcagccgtgc ctgcccgtcc tgcggaaaaa aggccacaga cctgtggata gcgacacagc
     3481 tgaatcgtct tcctgactgc gactgggtac acctggtctt caccctgccg gacacgcagt
     3541 ggccggtgtt cgaaagcaac cggtggctgc tgaatgacgt gtgccgtctg gcggtggaga
     3601 atctgctgta tgccgcccgg aaacgggggc aggaacccgg tatcttctgc gccatccaca
     3661 cgtatggccg tcgtctcaac tggcatccgc atgtacatgt gtctgtaacc tgtggaggtc
     3721 tgaataagca tggtcagtgg aaaaagctga gcttcctgaa agacgcgatg cgttcgcggt
     3781 ggatgtggaa tatgcggcaa cggcttctga aagcgtggtc agaggggctg gcgatgccgg
     3841 agtcgttgtc acatatcacg acggaatcac agaggagaag cctggtgctg aaagccggag
     3901 gaaaatactg gcatgtgtac atgtcgaaaa aaacggccgg cgggaggaat acggcgcgct
     3961 acctgggtcg ttatctgaag aagccgccga tagcggcctc ccggctggca cattacaacg
     4021 gagggggagc ctgagcttcc gttacctgga ccacaaaacg ggagaaacgg cgacggaaac
     4081 gctgacacag cgtgagctgg tcgcgaggct gaaacagcac atcccggaga agttttttaa
     4141 gatggtgagg tacttcgggt tccttgccaa ccgggtgtgt ggagagaagc tgccgcaggt
     4201 gta
//
LOCUS       DAJTNW010000196         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTNW010000196 DAJTNW010000000
VERSION     DAJTNW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.202"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..158)
                     /locus_tag="OO179_004041"
     CDS             complement(<1..158)
                     /locus_tag="OO179_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5533908.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO179_004042"
     CDS             complement(308..469)
                     /locus_tag="OO179_004042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533909.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO179_004043"
     CDS             complement(466..654)
                     /locus_tag="OO179_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5533910.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO179_004044"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO179_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5533911.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO179_004045"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO179_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO179_004046"
     tRNA            complement(2161..2237)
                     /locus_tag="OO179_004046"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO179_004047"
     tRNA            complement(2339..2414)
                     /locus_tag="OO179_004047"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO179_004048"
     CDS             complement(2559..3380)
                     /locus_tag="OO179_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5533912.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO179_004049"
     CDS             complement(3395..3751)
                     /locus_tag="OO179_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5533913.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4198)
                     /locus_tag="OO179_004050"
     CDS             complement(3764..>4198)
                     /locus_tag="OO179_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5533914.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1161 a         1136 c          826 g         1075 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc tctcccgg
//
LOCUS       DAJTNW010000197         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNW010000197 DAJTNW010000000
VERSION     DAJTNW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.203"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO179_004051"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO179_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5533915.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO179_004052"
     tRNA            complement(324..399)
                     /locus_tag="OO179_004052"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO179_004053"
     tRNA            complement(406..480)
                     /locus_tag="OO179_004053"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO179_004054"
     tRNA            complement(597..681)
                     /locus_tag="OO179_004054"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO179_004055"
     tRNA            complement(690..765)
                     /locus_tag="OO179_004055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO179_004056"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO179_004056"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5533916.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO179_004057"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO179_004057"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5533917.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO179_004058"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO179_004058"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5533918.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1040 c          929 g         1133 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa cccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJTNW010000198         4157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNW010000198 DAJTNW010000000
VERSION     DAJTNW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..322)
                     /gene="flhe"
                     /locus_tag="OO179_004059"
     CDS             complement(<1..322)
                     /gene="flhe"
                     /locus_tag="OO179_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5533919.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWE"
     gene            complement(322..2399)
                     /gene="flhA"
                     /locus_tag="OO179_004060"
                     /pseudo
     CDS             complement(322..2399)
                     /gene="flhA"
                     /locus_tag="OO179_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2392..3539)
                     /gene="flhB"
                     /locus_tag="OO179_004061"
                     /pseudo
     CDS             complement(2392..3539)
                     /gene="flhB"
                     /locus_tag="OO179_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3775..>4157
                     /locus_tag="OO179_004062"
     CDS             3775..>4157
                     /locus_tag="OO179_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5533920.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1036 a         1201 c         1020 g          900 t
ORIGIN      
        1 cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc acggtggtac
       61 cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc cgcagtcctg
      121 caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgaagcag
      181 gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt agcgtaattc
      241 cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata
      301 atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg
      361 gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc ggcgcaggaa
      421 gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg cacccagcat
      481 ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc
      541 cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg tatcgaggcc
      601 aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc gtcccaacgc
      661 cacgcgcacg acggcggtta attcatgtgg atcgctttgg atgggcgcat gttccgccag
      721 cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga gattttgcag
      781 cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg tcagctttgg
      841 catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa acagctctgc
      901 ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg cctcaaccac
      961 tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga tagcattcag
     1021 gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg ttccagggtt
     1081 aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc ctttcatcaa
     1141 aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca ctggcggcag
     1201 aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca acaactcacc
     1261 atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc ccagagaatc
     1321 ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac
     1381 cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg ccagccccag
     1441 caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa
     1501 accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat
     1561 ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg cggcggtaga
     1621 aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata gcgtataact
     1681 ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa cccgccgaca
     1741 acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa cttacttgcc
     1801 ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg ttttttcgcc
     1861 tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg cttacccggc
     1921 ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc tttggtaatg
     1981 accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc gaaattgcca
     2041 ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc gccggtatgc
     2101 ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa caacgtagta
     2161 aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
     2221 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
     2281 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
     2341 tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag attactcatg
     2401 ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag taggtgtaca
     2461 gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc cagcacttcc
     2521 gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg tcgatacagc
     2581 gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc aatttcacga
     2641 atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct cattttgttt
     2701 tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac atccgctttc
     2761 ggcacatcgg ccatcatccg acgccgtgcg gtagctcgct gcatctgaca gatccgtcct
     2821 ttaacatggg ggtcgccttc gccttgtttg aactcatcac gaatatcctg ccgcgacatt
     2881 cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac cattggaatg
     2941 acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt acccatggcg
     3001 gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca gagaaaaaac
     3061 cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc cgcgctagtc
     3121 tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa cttcggctgc
     3181 aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag cgccaccagc
     3241 accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag cagaataatt
     3301 tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag cccagcggag
     3361 agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac actaacgccc
     3421 accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc ctcttcccgc
     3481 gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc gtcagacacg
     3541 tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag cgttcaatgg
     3601 tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata ggttcagttg
     3661 cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc tatagaatcg
     3721 cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga tcaaatggaa
     3781 tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac caacatagca
     3841 tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga ccgattactt
     3901 tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt agatcaaaat
     3961 gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc tcgcgggata
     4021 ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc cacggggatt
     4081 gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca cgatctcggt
     4141 aatccaccat ttgggca
//
LOCUS       DAJTNW010000199         4117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNW010000199 DAJTNW010000000
VERSION     DAJTNW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.205"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..440
                     /gene="tnpA"
                     /locus_tag="OO179_004063"
                     /pseudo
     CDS             1..440
                     /gene="tnpA"
                     /locus_tag="OO179_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO179_004064"
                     /gene_synonym="qseD"
     CDS             complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO179_004064"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5533921.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1847..2566
                     /locus_tag="OO179_004065"
     CDS             1847..2566
                     /locus_tag="OO179_004065"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5533922.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2632..3930
                     /locus_tag="OO179_004066"
     CDS             2632..3930
                     /locus_tag="OO179_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5533923.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1041 a          894 c         1016 g         1166 t
ORIGIN      
        1 agcgcacacc cgatggaact gtaaatatca catagttttt tgcgccaaaa taccgaagac
       61 aggtgttcta cagagagaag cgtagagcaa caggcagtat tttgagaaag ctgtgtgagt
      121 ggaaaagcgt acggattctg gaagctgaat gctgtgcaga tcatatccat atgcttgtgg
      181 agatcccgcc caaaatgagc gtatccggct ttatgggata tctgaaaggg aaaagcagtc
      241 tgatgcttta cgagcagttt ggtgatttga aattcaaata caggaacagg gagttctggt
      301 gcagggggga ctacgtcgat acagtgggta agaacacggc gaagatacag gattacataa
      361 agcaccagct tgaagaggat aaaatgggag agcagttatc gattccctgt ccgggcagcc
      421 cgtttacgga ccgtaagtaa cgaagttgga tgcaaatgtc agatcgtgtg cgcctgttag
      481 ggcgcggctg gtaagagagc cttataggcg catttgaaaa acctccggct atgccggagg
      541 atatttattg cacgtcgtca attgcggcgt gctggctctt ccattgatat ctgctggatt
      601 aaatcgtctg gcatatgttc ctgtaaaata cgccagaggt tctcggcggt tctattcagt
      661 cgggtgttca ttcggtaaat ataagcctga atggggactg tcaatttagc agcatcaagg
      721 catatcagac gtttggtctg tagttcatcg acgatcgacc agatcggtaa ccaggcaatg
      781 ccgtatccgt tgagcgccat attctttaat aactcgctca tcgaagagat aaatatcgtg
      841 cgcccggtaa tacctccaac ctctgccaga tgacgattga ccaacctgcc catataagat
      901 gtggcggtat agttgagcaa aggaacctgg ggctgagtga tatcgaatac agggtgcccc
      961 tgtgcgtcgg cggcacatac cgggtacaac tcagattcaa acagtttcag gcagcaaaaa
     1021 ggcgattgca taagatcttc atcgcgaaac gaaataataa agtcactctt gccttcgcgt
     1081 aacgtattta cggcctggac aacatcaatg gcttcaacat gatagacaaa ttctccgcca
     1141 taggcggtaa gtgaatgcac cagtttgggt aatacgctca gggatagtga gtgcgcggct
     1201 gcaattttaa tgttgggaac gccgagtaaa ctctggccgt ttaactcccc caaattgcat
     1261 tcaagctgct gtaataagct gcgagtctgg gaatgaaaga gcttgccctc ttcggttaat
     1321 tgtaaagggg tggtcgtgcg atcgaataaa acaacgccga tcgctgcctc cagcgcctta
     1381 attcgtcggc tgaatgcggg ttgcgaaaga ttacgctctt ttgccgcctg agaaaaatgg
     1441 cgacaggctt ccagtgtcag gaaatcgtat aaccattttg tctcgatatt cttcattacc
     1501 atggcacctg tattttcaat ccaaagacat aataaaacat cccggcaata aaaataatgt
     1561 tctcaattag cacaacctgg cctttatcat tcgttttgtg cataacccct tcatttccgg
     1621 tattgacgtt gatatcacat ttcgccaaaa aaacgaaatt tttccgtcag ataaccagaa
     1681 tacatcatca ctatggtgaa taagcggaat gttccatagc tgcatcatgc gtgtcttgca
     1741 tcgccttgta caaccttgca tttctcatcg cccatttcga ctgttaaaaa agagagcagc
     1801 agttatttac cgcgtattgt cattaacgtt ttgagaggca ggtattgtga aaggtttaat
     1861 tggtgtactc ggtggaatgg ggccggcagc taccgtcgac ctgtttaata aatttgtaac
     1921 ttttaccgct gcacaatgtg atcaggaaca tattccttta attatttctt cgatcccgga
     1981 tattcctgac cgtaccgatg cattaatgca tcatgggcgt tcaccattac ccgttatgcg
     2041 tgattatatg cataagctgg aagatgccgg ggcagaatgt attgttattc cctgtaatac
     2101 cgcgcattac tggttcaaag aattaaaaga tgcttgtcat attgatatat taagtatcgt
     2161 agaaactacg atcaatgaag ttcgcgcctg tggtaaaacc cgaattggct tgttagccac
     2221 caatgccacg ctatatatgg gtttatacca aaaaggaatt gagtcgctgg gatttacctg
     2281 tgtcagcccc gatgccgacg gtcagaaaaa agtgatggaa agtatttatt ccttgaaagc
     2341 tggcaatata gcgcacgcac aaaagttgat gaatgagcag gctgaaatat tattttcccg
     2401 cggtgcagaa atccttgttc ttggttgtac agaaattccg gttattctgg cgcaagccgt
     2461 taaagagcag ccttcccgct atattgactc aacggcgtca ctcgttcgtg ccggaataaa
     2521 atggtacgaa aatcgtgtcg gtaaacatta tcttttaacc caataatcat ttaaatcgca
     2581 gccagaaagt tattcgcttt taactgaatt atatttataa cggagaacat tatggtttgg
     2641 ctggaaatta tcgtagtact tggtgcaata ttttttggta ttcgccaggg gggaatcggt
     2701 attggtttat gtggcgggct tgggcttgcc attctgactc tgggatttgg tctgcctatg
     2761 gggtcgccgc cagtcgacgt gatcctgatt attatgaccg ttgttgtggc ggcctcagcc
     2821 ttacaggccg caggcggaat ggattatctg gtacgcgtcg ctggtaattt tatgcggcgt
     2881 aacccgaaat atatcaacat aatcgcgccg attatcacct ggtctatgac cattatggcc
     2941 ggtactgggt ttattgtttt ttccacgctg ccggtgattg ctgaagtggc gaaagagtcc
     3001 ggtatccgtc cttcccgcac gcttgccggt tcggttgtcg cttcgcaggt tgcgatttcc
     3061 ggttcgccga tcagcgccgc aatggcggct atgctcacta ttatggaggt aaacggtgtc
     3121 agttttattc aagtgatgtc ggtatgtctt cccacctctt ttgctgccgc gatggtcgcc
     3181 gcttttattg cttcccgaca gggatgtgag ttgcaggatg acgaggtcta ccttgagcgc
     3241 ctgcaaaaag gactggtgca aaaatacgag aataatagca gcatcaagcc aggtgcgacg
     3301 ctctccgtcg gattgttcct gctggcgacg gtcgctattg ttattcttgc tgccttcccc
     3361 caactgcgtc cgggttttga tatcggtaaa ccgatggaaa cacgcgatat cattattatc
     3421 tgtatgctct ctgccgcctg cctgatggtg gtgctgtgta agacggcgac cgatgccatt
     3481 attctcacct ccacattccg tgcggggatg agttcgctgg cggtaattct cggcattgta
     3541 accttaggca ccacttttat tgatgcgcat ttaactgaga ttaaagatat tgctggtgat
     3601 attttgcagg cgtatccgat gttgttggcg ctggttcttt ttggcacttg cgcgctgctc
     3661 tactcgcaag gtgcgaccac gccgttaatt atcccgcttg ccgtcgcgct tgacgtgccc
     3721 acctgggcaa ttctggcgtc ctatgtggcc gtcaccggag tatttgtact gccaacgtat
     3781 ccaacatcac tggctgccat ggaatttgat actaccggca caactcgtgt aggcaaatat
     3841 gtgctgaatc atccatttat gctgcctggg ttggggggaa ttattgctgg agtggcatta
     3901 ggctttgtga ttgcgccaat gattatttag cactatcaaa catcagtatc gatggtttat
     3961 acacaataaa gacagatatg gcggaatata gtctgttgta agcgccaaca aagaataggg
     4021 gaattccgcc atgtctgaac atcttcacaa tatggtatta agtgttgtcg tttaatgatt
     4081 tatttcttgg ttatgttgtg tattagctgc atctatc
//
LOCUS       DAJTNW010000200         4110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNW010000200 DAJTNW010000000
VERSION     DAJTNW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.206"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(954..1145)
                     /locus_tag="OO179_004067"
     CDS             complement(954..1145)
                     /locus_tag="OO179_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5533924.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="OO179_004068"
     CDS             1209..1532
                     /locus_tag="OO179_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5533925.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="OO179_004069"
     CDS             1522..1833
                     /locus_tag="OO179_004069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533926.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="OO179_004070"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="OO179_004070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="OO179_004071"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="OO179_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="OO179_004072"
     CDS             2748..2981
                     /locus_tag="OO179_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5533927.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="OO179_004073"
     CDS             complement(3527..3994)
                     /locus_tag="OO179_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533928.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          999 a          938 c         1193 g          980 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtacggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacagc ttgtctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAJTNW010000201         4049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTNW010000201 DAJTNW010000000
VERSION     DAJTNW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.207"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            41..163
                     /locus_tag="OO179_004074"
                     /pseudo
     CDS             41..163
                     /locus_tag="OO179_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(425..991)
                     /gene="ipgB2"
                     /locus_tag="OO179_004075"
     CDS             complement(425..991)
                     /gene="ipgB2"
                     /locus_tag="OO179_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5533929.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1273..1587)
                     /locus_tag="OO179_004076"
     CDS             complement(1273..1587)
                     /locus_tag="OO179_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533930.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1665..1775)
                     /locus_tag="OO179_004077"
                     /pseudo
     CDS             complement(1665..1775)
                     /locus_tag="OO179_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2099..2776)
                     /gene="ospD1"
                     /locus_tag="OO179_004078"
     CDS             complement(2099..2776)
                     /gene="ospD1"
                     /locus_tag="OO179_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5533931.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     LLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3147..3284)
                     /locus_tag="OO179_004079"
                     /pseudo
     CDS             complement(3147..3284)
                     /locus_tag="OO179_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3366..3664)
                     /locus_tag="OO179_004080"
                     /pseudo
     CDS             complement(3366..3664)
                     /locus_tag="OO179_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1119 a          827 c          759 g         1344 t
ORIGIN      
        1 agcagccgga gagtgaaact ctctccggct gactcgactc gcaggttccg acagcggtga
       61 cgaacatgca gcggtgttgt tctcgctgat cggcacatgc cgtctgaaca atgtggagcc
      121 agaaaaatgg ctgcgttacg ttattgaaca tatccaggac tgaccggcaa accgggtacg
      181 cgatctgttg ccttggaaag ttgatctgac ttctcagtaa atatcaatat gattctgacg
      241 agccgcttac ctttgagcag agaaagggga gagggagccc acatccatga gctaggaaaa
      301 tacaaaaact ggctatacaa caattcaata tattaggact tacttgcgag gagaaatata
      361 gcaccgctca ggcaattcct gggtatttac ttttcttagt ttctttactc gggatgttct
      421 gtcatcagaa aggcgattct aaatttgtaa tatagtcact catattccca cgtaatttct
      481 ttagatcaat atattgcaca tttttctcaa aaaactgatc ggaaataaga tttgcaacat
      541 tgcttgttgt atcattttta acgcttctat tcatcccttc acaaagttcg tccattttct
      601 ttttgaaata actgcttcct gatacaagat gatttatgga actttgtgca gcattgatgt
      661 ctagagaaat gcgataatct ttctcaacct gtttaaatat ctgtttacgg agattatcgc
      721 cgatttttcg gctgtgttct gcacaaaact tatcaataca ttggtttacc acccgggata
      781 cgtagacttt tttttcatca tttttccaat ctgtaatatt ttcaccgatt tgttctgaaa
      841 cagagctcaa aatactagta tttattttgg ttgatgatat ttttactatc cttttcccca
      901 ttgtacagcg gataatctca tccacctttc ctgaaaaata tcttttgtga gataaactga
      961 ttccaaaatt attaaaagat gttccaagca ttataatcac cttgtcatgt aaaatagtaa
     1021 cattgtcatt attttagctg attcaaacaa ttctgtaaat gctttgagtt ctttactccc
     1081 ttggctcaat gatggaccgt ctggaatccc agagactaaa ctctctgaga aagagagtat
     1141 gaaaaaactt ggggcagtcc ctaaatgaag ggaccaacag cggtaaaata accggcatca
     1201 ttcataatga tgccctgtta gttgtagtta tgaagattta cgtcattaaa attgctgttc
     1261 gtagtgtcag tcctatggtc tggcatcagc tgaggatcgt agccgataca tcactggctt
     1321 cacgacattc gtgttgaggc tattcatgaa aactccacgc tgaaaacgcc gttttgtata
     1381 aacggtcacg gtatgcccgg agccactgtt gctgatgaat tcgataaaac cttggcgttt
     1441 cttgaagcta tcgttaatgc ggataatgaa acaacgattg gtgagattcg ctcttttgcc
     1501 gacgccctgg acgctgtccg gttcaatcgc aacaaaatca accggcagct gagcaaaccc
     1561 aaccttgcct ctctggcgct ggagcatgaa gtgatttggc tgggacgtag ccgctgacag
     1621 cttctataaa agtatgatcc tacccacgta atatgaacac aggcttaagt gaggttctgg
     1681 ttttcaaatt gttcaggact gagacctcca caccaactgt gccgtcgtca gcgattgtaa
     1741 tcgcacttga tataattaaa cactgtatat ttcatttgtg gagtctccag tctgagaggg
     1801 ctttatgcat tccgtattgg gcactatgat gccagaaatc tgcaaggctc cacaccttcc
     1861 tgtatttccc ttcctctatc acagtgcact ttacttaata gggtgggatg tgttcattac
     1921 atttattgtg ttttttatga ttgtttttgc gtccagtgtc gttcgtcatg ggaaactggt
     1981 tctatgggta gtatcgctca gtctagttaa ctgtttgttt ctttttagaa attaagcaga
     2041 tcacacaatt cggggagaga tccttctaag tgcaattctt tgcagctatt tagcacactt
     2101 aaaacaactc ttgtattttg tcagaaataa tattaaacat gtcttgttca ttattgttaa
     2161 gtctgtctgc tatttctgta agttttatcc cactatgatg gcatatattt atatatgcca
     2221 tcatagcctc gatattccct ttccctgcgg ccagaaataa tcctggtaac ccctcattat
     2281 ctcttacatc caatagttta atcgtttctt ttggtgataa atcaaaagtt ttaataaaat
     2341 ctccataagc ttgaatcgca gcggaatgac cttcttgaag agctatgaac aaagcagatt
     2401 ccccagcatt atttcgtgct gcaagaactt ctagtttttc ctctttgggt atttcggctg
     2461 ccaacagcaa ttcaccgtat ttacgcgctg catcggcatt tccatgccaa atcaatgaaa
     2521 acaaattatg gcggttatac tccttattat tctttgtcgg aatggatatg ttctgtattt
     2581 tggccaattt tctgaaagaa acaccactat atcccccccc tgatttttct tcattgatgg
     2641 cgtgggatat agcggtattt gtcacgttaa tgatattatt tttcattcct ttattttcat
     2701 tagcagtatt gctgcctatt attaggtgca tatttttgtt gtttgctgga tgtaatccat
     2761 agttatttat tgacattttt gttcatcctt tttgctaaaa aaaacaataa cctttttttg
     2821 taatgtaata gatattagtc tattgctaat ctaaaaaaac cattgccaca ttgaatggct
     2881 atattcacca ataaattctt tcgatttcgt atattgacat atcgtagata tatcttatcc
     2941 ctaagattca ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac tcagaagtga
     3001 ccgtcaaggc gatcaccaaa cttgataata aagcagctca ttgccatacg ccagtcctta
     3061 aggggcgttg tccatttctg tgatgctgcc tagatttaac tgccagctcc ggcttatcta
     3121 catctaaata tgtgagccta ataccgctac actttgtcca gactatgttt ttctccggga
     3181 agtgggctaa ccgtctccat aaagtgacga acttttttca acagtggcca tattgaacgg
     3241 cactgatgat tacgggttat tgtctcatga agagccggcc acatcactga cagcagtgac
     3301 ttcaggcctt cagcaccaga cagcctgaac cagttaatcc atcgcccgac aaatttagta
     3361 atctagggct ggtttttctt cagctgttgc ggttcgaaag tgccattacg atcacgcaga
     3421 gtacggattt ccagaggttc atctccagta gtgataatct tcgttaagca accattgcgg
     3481 gaattgctgc cagatctaag ctgatttttc tcgcacccaa gatgatgagt catattagca
     3541 ttgagtacgg tttcattttc agcatccaat caaactgact gagatcttca ggatttttga
     3601 gacttttggc tagttcatta gctaaagccc gcaacttatt agatgtcaac tgtttttcat
     3661 tcataaattc accttcattt gatatcaaat taaatagttc aacatcagag aattatacaa
     3721 acttatgtac aggctcctat gatcttcgaa tcctgaagat tatagtgaaa ataagttttc
     3781 acgttgaaat cccacatagt tagtatatga taaataatca tggatgacct gattaacctc
     3841 ttggggtagt gcttttatag aagaataaat attctactaa tttaatacat cacctaaaat
     3901 agtattgttt tttatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     3961 ttccagtgcc agatcttact tataataaag gggcttggca gtttggataa actattgttc
     4021 ttattccact aatacaaact ttttgtaaa
//
LOCUS       DAJTNW010000202         4016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNW010000202 DAJTNW010000000
VERSION     DAJTNW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..313
                     /locus_tag="OO179_004081"
     CDS             <1..313
                     /locus_tag="OO179_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5533932.1"
                     /translation="TAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSG
                     VSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNA
                     R"
     gene            575..937
                     /locus_tag="OO179_004082"
     CDS             575..937
                     /locus_tag="OO179_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5533933.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            934..1854
                     /locus_tag="OO179_004083"
     CDS             934..1854
                     /locus_tag="OO179_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5533934.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1857..2808
                     /locus_tag="OO179_004084"
                     /pseudo
     CDS             1857..2808
                     /locus_tag="OO179_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2835..3075
                     /locus_tag="OO179_004085"
                     /pseudo
     CDS             2835..3075
                     /locus_tag="OO179_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3072..3377
                     /locus_tag="OO179_004086"
     CDS             3072..3377
                     /locus_tag="OO179_004086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533935.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3611..>4016)
                     /locus_tag="OO179_004087"
     CDS             complement(3611..>4016)
                     /locus_tag="OO179_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5533936.1"
                     /translation="SYFYSASTNGFYSTEFHGTNIPDDAVEISESEWKTLINSQSVTK
                     MITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQDAVDLDMATDDE
                     TKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1165 a          781 c          777 g         1293 t
ORIGIN      
        1 aacagcagcg gtaaggaaga tgagaaccga cagcaacaag gcatggaagg gagcgttaaa
       61 gcgggcaggc attagcaact tccgcttcca tgacctgagg catacctggg caagctggct
      121 ggttcagtcc ggtgtctctc ttcttgcgct taaagagatg ggaggatggg aaactctcga
      181 aatggttcaa agatacgccc acctttcagc cgggcatctc accgagcacg cgagcaaaat
      241 cgatgcgatt ataagtcgca atggcacaaa tacggcacaa gaggagaacg tagtttactt
      301 aaatgcgagg taacttattg atttaaatgg tgccgataat aggagtcgaa cctacgacct
      361 tcgcattacg aattataaga atccgcttgt aattcaaagg attatcccat caacactgcg
      421 ctcacacgtc ccaccacatc aaaacatgta aagccttgca agccattgtg aggccttatg
      481 tgtctcagtt ttgtctcatc agacatagca agtatcgatc aattgagact tggatgatag
      541 acttcatgcc tttcagagct cattgattaa ataaatgtta aagttatttg taaagtacac
      601 atctataggt gtgctgaaca cacttataca ctgggtggtt tttggtgtat gtatctatac
      661 cgcacatacc agtcaggctc tggcaaactt cacaggtttc gtagtggctg tgagctttag
      721 cttcttcgcg aatgcaagat tcacattcaa agcatcgact acagcgatgc gctacatgtt
      781 atacgtggga tttatgggaa tattgagtgt gattgttggt tgggcagctg ataaatgctc
      841 acttcctcca atagtcactc ttatcacctt ctccgccatc agtctggtgt gcggtttcgt
      901 ctattcaaag ttcattgtct ttagggatgc gaaatgaaaa tatctcttgt cgttcctgtc
      961 ttcaatgaag aagaagcgat acctgttttc tataaaacgg tacgtgaatt ccaagagttg
     1021 aagccatatg aagtagaaat tgtattcata aatgacggaa gtaaagatgc cacagagtca
     1081 attattaacg cgctggctgt ttcagacccg ctagttgttc cgctgtcatt tacacgcaac
     1141 tttggtaaag aaccagcctt atttgcaggg ttagaccatg caagcggcga tgctgtaatt
     1201 cctattgatg tcgacctgca agacccaatt gaggttatcc ctcatcttat tgaaaagtgg
     1261 caggcaggtg ctgagatggt gcttgctaaa cgttcagacc gctcaactga tggacgactg
     1321 aaacgtaaga cagctgagtg gttctataaa ttacacaaca aaataagcac cccaaagatc
     1381 gaggaaaatg tcggagattt tcgactcatg tctcgtgagg ttgtggagaa cattaaactg
     1441 ttgcctgagc gcaatctttt catgaaaggc atactgagct gggtgggtgg tcagacggat
     1501 gtcgttgaat atgtacgcgc agagcgtgtt gctggcatct caaaatttaa tggctggaaa
     1561 ttatggaatc tggcactgga aggtatcaca agtttttcaa cctttcctct tcgcgtatgg
     1621 acttatatag gcttgtttgt tgcaagcatt tcatttttat atggtgcatg gatgattata
     1681 gacacccttg tctttggtaa cccagtacgc gggtatccct ccctgcttgt atcaatactt
     1741 ttcttgggtg gagtgcaact gatcgggatt ggtgttctcg gtgaatatat aggtagaatt
     1801 tacttagaaa caaaatcaag acctaagtat attttgaaga aaggtagaaa ataaccatga
     1861 gcatatgtat taaacaatca gcattaaaaa tattattggc tttatcagca ctcctaataa
     1921 cttggttgac taccagatac tttccagttg aaccggatgt tgctaacagc ccaattgtat
     1981 ggaggcatat tttagagaat ggtatatcat caatccacga ctggaaacca accgtggata
     2041 attggtattt caccgtttac ccaattaact ttttatttta tatgctgtta ggtgatgatg
     2101 gcttagtagc attaagatta tcaactgcag ttttctctat tgctatagta atagcagcga
     2161 tgttaacgct acgtaaggca ttcgggttta cccctgcttt attttctatt atacttctat
     2221 ctctaatacc atatttttca tacacttatg gttttgtatc acatcctttt tctcataatt
     2281 caacaaacgc tttcggcttt ttatgcttgc ttatatctgt tttcaacata cagtataaaa
     2341 atatatttat cactctcctc ttaagtttga cagcattatt ttccagcgta tccgatccgt
     2401 ggtttacagc tgcatttttt attccactat taatatctta ctttttattc tctgtatggg
     2461 ataaaaaatt atttaagcat actgcattaa tactatttgc atgtttaatc tctctttcca
     2521 atgtattgca aaatctactt aacatacctc cccaccaatt tgaaattgta tcactcaatg
     2581 atatgatttt aaatgcaaaa tggtgcattc tgttaatagg aaaaagtctt aatttattag
     2641 ttgtcgacaa taacgcaaca tcttacgcct catttgtcat ttggttcatt gccattatta
     2701 catctgcgtg gtttgtttta tcggacaata aaaaaaacac ataccgtatt tatattgtgt
     2761 tattttcatt attatctatc gcaggtattg tctcatcatt catactgagc tataaatccc
     2821 ctgattatat tagcatgcgt ttctttatga atgttacctg tttcgcactg atattgtgct
     2881 gtatcggtac atcaacaaaa gcaaagatat tattttatct aatcgcattt ttattttcga
     2941 tcagttcaat taaatcttac acaaataatg cttcaccatt acacgatcag gagaagattg
     3001 ttaaatcata cattgatttt ttaaaaaaaa ataatctcca ttatgggtac ggctcttttt
     3061 gggatttgtc aatgactgta aattggttat ctggtgggga catacagata actcctgttt
     3121 tctttaatgc tgattcgggg aaaattaact ttactggcgt gagacaacag acactggctt
     3181 cttggcatag caaggaggcg ttcaattctg ctcctgagcg acagtttata gcagtttcca
     3241 tagctaacga acctgaacga tgtaaagaaa tgacatcctg ccttgcaggt attcaagaac
     3301 aattaggtaa gcctgacgaa gttttgaact ttgaaggaag agtaatatta gtatttaaca
     3361 aaaaacttaa cctatagtaa attgccccct accatataaa ccacactaac gaatgaataa
     3421 cccgtctttt tcgacgggtt atatccctat caggaataaa aatggagtgg ttgccgtata
     3481 caacaaaccc tcatttagag ctatattcta gagtatatga gatactataa agttgaagga
     3541 gctatcatta gaaaaaccat atgctgttat aattagatgt ctcgcttttc atccaataac
     3601 gaattttctc ttatagattg ctctctggtt ttttcggcca ctctgtattt tttatatcaa
     3661 cacgcatcaa tagtgcgcga tattttttcc atgccagtaa cagtttcgtt tcatcatctg
     3721 ttgccatatc taaatcaacc gcatcctgaa gcggcgctat tacattcgtt gcctctgcta
     3781 tcagtgcaga tttcttttca ttatttatta aggctaattg ttctggtgtt ggagaaggac
     3841 ggtcaacaat gacagggtga ccattctcac cacaggtaat catttttgtt acactctgcg
     3901 aattaatcag tgttttccac tctgattccg agatttccac tgcatcatca ggaatattgg
     3961 tgccgtgaaa ttcagtcgaa taaaatccgt ttgtcgatgc agaataaaaa tatgac
//
LOCUS       DAJTNW010000203         3946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNW010000203 DAJTNW010000000
VERSION     DAJTNW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.209"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            487..1899
                     /gene="ospC1"
                     /locus_tag="OO179_004088"
     CDS             487..1899
                     /gene="ospC1"
                     /locus_tag="OO179_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5533937.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2003..2152)
                     /locus_tag="OO179_004089"
                     /pseudo
     CDS             complement(2003..2152)
                     /locus_tag="OO179_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2228..3925
                     /gene="ospD3"
                     /locus_tag="OO179_004090"
     CDS             2228..3925
                     /gene="ospD3"
                     /locus_tag="OO179_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5533938.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1430 a          654 c          687 g         1175 t
ORIGIN      
        1 actgctccat taaggcgatt ttgaactcat aagggaagta acaaaaaaac tgcaaaagta
       61 caataattat atatggccaa actaacctct attaaaaaac atcagttttt cacacttagt
      121 tgagcttcat gaaaaaatca tcgaggattt cattgcagaa tacacattcc actaactgga
      181 agcactccat aagaggaatc agagaaagta tcgttttttt atagtaaaat tcattgctgt
      241 tcaataaaat tgatatagtt attaaattat gaaaataatt tcacaaaaaa gattaacttg
      301 agttcttaag catcggattt tagatatttt gaaatatctg gcatcacata acatcaaaag
      361 tatataatac ccttatctaa ttacatgcag tatattgtat atatactcaa aaattgtaat
      421 tcataccctg ctatatcttg tagggaaaga tattaattaa aactgttttc atataaggtt
      481 cattttatga atatatcaga aacactgaac tcagcaaata cccaatgcaa tatagattct
      541 atggataaca gattacatac attgtttcca aaagtgacat cagtgcgaaa cgctgcacaa
      601 caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa atattattaa agatttcttt
      661 aggaaaacta tagcagcaca gagttatagt agaatgttct ctcaaggctc taactttaaa
      721 tctttaaata tagcaattga tgcaccatca gacgctaaag cctcatttaa ggctattgag
      781 caccttgaca gattatcgaa gcattatata tctgaaataa gggaaaaact tcatcctctt
      841 tctgcagagg aactcaattt gctttcgcta attattaatt ctgatttaat cttcagacat
      901 caaagtaatt ctgatttgtc tgataaaatt ttaaacatta agtcattcaa taaaattcag
      961 tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg atataaaaaa aatagctaat
     1021 catgactttg tgttttttgg cgttgaaatc tctaaccatc agaaaaaaca ccccctgaat
     1081 acaaaacatc acactgttga ttttggtgca aatgcgtata tcattgatca tgactctcca
     1141 tatggatata tgacattaac cgatcacttt gataatgcta ttccacctgt tttttaccat
     1201 gagcaccaat catttttaga taaattttca gaggttaata aagaagttag tcgatacgta
     1261 catggaagta aaggaattat agatgtacca atattcaata ctaaagatat gaagttaggg
     1321 ctcggattat acctgattga ctttattaga aaaagtgaag accaaagctt caaggagttt
     1381 tgctatggaa aaaatcttgc ccctgtggat ctggatagaa tcataaactt tgtttttcag
     1441 ccagagtacc atatacctag gatggtaagt acagaaaact tcaaaaaagt taagattaga
     1501 gaaatatcct tagaggaggc tgttacagca tctaattacg aagaaattaa caagcaggtc
     1561 actaacaaaa aaattgctct ccaggctctt tttctttcga ttactaatca aaaagaggat
     1621 gtcgccttat atatattatc taattttgag ataactagac aagatgttat ttccataaag
     1681 catgagttgt atgatattga gtatctactt agcgctcata attcaagctg taaagtactt
     1741 gagtatttta tcaataaggg attggttgat gtaaacacaa agttcaaaaa aactaatagt
     1801 ggggattgta tgttggataa cgcaataaaa tatgagaatg cagaaatgat aaaactatta
     1861 ttgaaatatg gtgcaacatc tgacaataaa tatatttaat caaaattgaa tatcgtttag
     1921 caaaaggcag tatttgcaca aaatatcgat atattgttta ttgtcagtat ggctcaatgt
     1981 gataatagct ggaaagtttg atgggtttcg ccccgttgta gcggtagtcg aactggaagg
     2041 tcttcaggca ctgcttacag cgatagagca gcccccctga actggaatgg ccgttccgga
     2101 tacccccctg agtttcagag taacggtgac aaaccacatc aatctttgcc atcaatcatc
     2161 caaagggcaa agagtacaac aacactaagt ctgcgtcaca acccatcaat gaaaggaata
     2221 tatacatatg ccatcagtaa atttaatccc atcaaggaaa atatgtttgc aaaatatgat
     2281 aaataaagac aacgtctctg ttgagacaat ccagtctcta ttgcactcaa aacaattgcc
     2341 atatttttct gacaagagga gttttttatt aaatctaaat tgccaagtta ccgatcactc
     2401 tggaagactt attgtctgtc gacatttagc ttcctactgg atagcacagt ttaacaaaag
     2461 tagtggtcac gtggattatc atcactttgc ttttccggat gaaattaaaa attatgtttc
     2521 agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt atttattttg ttgaaaacgg
     2581 ttcatgggga gatattattt tttatatttt caatgaaatg atttttcatt ccgaaaaaag
     2641 cagagcacta gaaataagta catcaaatca caatatggca ttaggcttga agattaaaga
     2701 aactaaaaat gggggggatt ttgtcattca gctttatgat cccaaccata cagcaactca
     2761 tttacgagca gagtttaaca aatttaactt agctaaaata aaaaaactga ctgtagataa
     2821 ttttcttgat gaaaaacatc agaaatgtta tggtcttata tccgacggta tgtctatatt
     2881 tgtggacaga catactccaa caagcatgtc ctccataatc agatggcctg ataatttact
     2941 tcaccccaaa gttatttatc acgcgatgcg tatgggattg actgagctaa tccaaaaagt
     3001 aacaagagtc gtacaactat ctgacctttc agacaatacg ttagaattac ttttggcagc
     3061 caaaaatgac gatggtttgt caggattgct tttagcttta caaaatgggc attcagatac
     3121 aatcttagca tacggagaac tcttggaaac ttctggactt aaccttgata aaacggtaga
     3181 actactaact gcggaaggaa tgggaggacg aatatcgggt ttatcccaag cacttcaaaa
     3241 tgggcatgca gaaactatca aaacatacgg aaggcttctc aagaagagag caataaatat
     3301 cgaatacaat aagctgaaaa atttgctgac cgcttattat tatgatgaag tacacagaca
     3361 gatacccgga ctaatgtttg ctcttcaaaa tggacatgca gatgctatac gcgcatacgg
     3421 tgagctcatt cttagccccc ctctcctcaa ctcagaggat attgtaaatt tgctggcctc
     3481 aaggagatat gacaatgttc ccggacttct gttagcattg aataatggac aggctgatgc
     3541 aatcttagct tatggtgata tcttgaatga ggcaaaactt aacttggata aaaaagcaga
     3601 gctgttagaa gcgaaagatt ctaatggttt atctggattg tttgtagcct tgcataatgg
     3661 atgtgtagaa acaattattg cttatgggaa aatacttcac actgcagacc ttactccaca
     3721 tcaggcatca aaattactgg cagcagaagg cccaaatggg gtatctggat taattatagc
     3781 ttttcaaaat aggaattttg aggcaataaa aacttatatg ggaataataa aaaatgaaaa
     3841 tattacacct gaagaaatag cagaacactt ggacaaaaaa aatggaagtg attttctaga
     3901 aattatgaag aatataaaaa gctgaatatt atctcgtggt gataca
//
LOCUS       DAJTNW010000204         3651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTNW010000204 DAJTNW010000000
VERSION     DAJTNW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..140
                     /locus_tag="OO179_004091"
     CDS             <1..140
                     /locus_tag="OO179_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5533939.1"
                     /translation="SLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSS
                     Q"
     gene            complement(170..652)
                     /locus_tag="OO179_004092"
                     /pseudo
     CDS             complement(170..652)
                     /locus_tag="OO179_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(908..1888)
                     /locus_tag="OO179_004093"
     CDS             complement(908..1888)
                     /locus_tag="OO179_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5533940.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(1888..3087)
                     /locus_tag="OO179_004094"
     CDS             complement(1888..3087)
                     /locus_tag="OO179_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5533941.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(3409..>3651)
                     /locus_tag="OO179_004095"
     CDS             complement(3409..>3651)
                     /locus_tag="OO179_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5533942.1"
                     /translation="ALLTPFCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIARNNRT
                     LRTRIKRLARKTICFSRSVEIHEKVIGSFIEKHMFY"
BASE COUNT         1007 a          844 c          775 g         1025 t
ORIGIN      
        1 actcgctgat cggcacatgc cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg
       61 tcattgagca tatccaggac tggccggcaa accgggtacg tgatctgttg ccctggaaag
      121 ttgatctgag ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta
      181 attctgctgc gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat
      241 ttatcagttg tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtctggacgt
      301 cctgttgctc cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag
      361 ctattgcctg tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt
      421 gtgccggaag caatattaca tcatggtcga gtttcctgat ttcccgggcc cagtaatgag
      481 atgccccgcc agcctccatc gcaacagtca ttttaggctg ttgcaccagc cagttaagca
      541 gttgattgcg agaaagtttg gtctttcctg cgggtttgcc atgagcattg atactgaaaa
      601 gataaaagtt cgttttagcc agatcaatgc cggtgatttt ctgttcagac atggtatccc
      661 gcttactttt gttgttaaag tttatccatc atggcgcaat tgaaggctgc cgtgaaagag
      721 ggggaagact atctcatcgg ttactgttaa acggctaaga gaaactatct ttttcaagca
      781 aagaagatac ggtgaaacag gcgatttcac cgtgaaatac tgttcaaaaa aaacgctaat
      841 aaaagcaaaa tttcttttaa aacagccaat taaaaactgg tgagttgctg gcgaaaaact
      901 tgaattttca cagcgaattc agattatctt ttagtacaag agcaatcata cgatctaact
      961 tatcctgaac ttccaacggt aatctgccga attcatatga gaacgtacgt ccctttactc
     1021 gttttcgagc aaatttatct ttactatcaa atttccataa caaagttaca accgctttat
     1081 cctttactcc tgaatcaagc aatgaagaca tttccttggt tataagccgc ataatactgt
     1141 ttttaacctc gtcttcagaa agattgtcat cagcagtaat aagctcaata ctcggcgaga
     1201 tattcttcac aacttcatca atagatatat cacccagcct cagtcgttca cttaattcag
     1261 ccagttgcct gtaatcggcg tatgtcagct cagactgaac aggaaaaaga gacacaaaat
     1321 ctttaggtac ggacgctgct tggagggcac gagtcacctt agctgccgac aatccctctc
     1381 tttccgcaat ttgcttctgg ttcataccgg cctctttgag acgtactaaa cgcaacccaa
     1441 tttcacgaat attatgttca agtgacgtct gcaaatcttt cgccagatgc tgagcttcag
     1501 aaacagtaag ctcatcatcc gttacaagta cacgtaatcc aaccttgcat agcaaagctg
     1561 ctgcccgacg acgggaacca tccaaaatct caataagatc gccagtccga atcccaatac
     1621 atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc ttttagtgag tccggcgtca
     1681 gggctgtctg ttcgcggcca ttcacctctt caaccacaaa agtttgagta tccactttgt
     1741 catgatcaac cttgatctca gtaaattttg cctttcggcc agtattgagg gtaaatacat
     1801 gaactggagc cgatatttct tcagtattat tcaggatatt tgtattcagc gtacggccaa
     1861 tagtcggtcg gtgctttctg ttttccatca gtagttggcc cttataaatt caatacgatc
     1921 aaaaactgct ttagcgaaat cttctgctgc catacgtgca tttttcaaag cttcaccact
     1981 gccaacataa gtaacaggat tcgcagatat cacggtatca aacgactcgc cgcttctctc
     2041 aaaaccatca agacgtggca ttgaaacatc aagcatgtca ccaccgaaaa tctcttttgt
     2101 caggctatgg cagtatttat gatctgactt attcgccagt ttcgacatga agccaatatt
     2161 tgcctgcaaa cggcatgaac atccatcctg ttcaataatc tgtacaagtt caggtaaacg
     2221 agccaaatat ttaagcgtgg aatggaaatc aacttgtgct ggcggcaccg gtgtaaacat
     2281 gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc agatgcgggc ctgtatcaat
     2341 aaggatgaag tcgaaatcat gcttcagctt atcaataata ttctcacgca atatcgcgtg
     2401 tttgttttgt cctaatagat gctcctcgca cagagtatcc cagtttgatg caataaaggc
     2461 gtcgtcaata gaagccggca taacataaac gccagggata actgacggaa cgataaaatc
     2521 ttccagtaat tcctcacgac tcacgttctg caacatagcc tgcggtgcgg ttgtatcaac
     2581 cagccccacc gcatgcaagt agttcagaaa catagtggct gatgactggg gatctaaatc
     2641 aaggagcaga atacgtaaat cttctgacag caggtgagga tgagcacgca gggcgtgagc
     2701 tacggaaaca ctactcacgg ttttagacac accgcctttt agactaccga tgaatatgga
     2761 atatgcctca ctataacgat cgcgatattt aggaatgcca cgatgtgcat agatatcgat
     2821 aatgttctgg atggttaatg catattttgt cgcggttcct gctgggcgtt tttcaaactg
     2881 atagccctga gcctccatct caccgactgc accatccacg cttgcgcgcg ttaattttgg
     2941 aagcttagct acagcagact ttgaatagac ttgataaaaa acatcagcct gaaactcctg
     3001 cttttgctcc tgaatctgtt ttgttagggc gagcaacatt ttatctgcac gctgcgcaac
     3061 cttacttaac tgctcaaaac tagtcattcc ccctccgtgc tatgtaggat tttaacttat
     3121 tacaaaaaaa actgcattag cagggtaatg catgcaagta catattgtac atgcttatat
     3181 taaaaatgcg cgcattgctg atttattaat catcggaatg aatattactg taagatagta
     3241 tgcctttccg ggagaaagac gctgatttcc cccgaataat ccagttcacc accacactcc
     3301 ggacaacagc tttcttcagg ctccagatgg tgtatttcgc gggaaacatg ttccggtaac
     3361 ggacggcgat gacgtgactg tcgcaacagg cgggggtagt gcttccaatc agtagaacat
     3421 gtgcttttca atgaaggagc cgatgacttt ttcgtggatt tccacggaac gcgagaagca
     3481 gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg gtccggttgt tacgtgcaat
     3541 acgttgcgtg aagattttgc cggtcaggtg tttctccttc ggcacttctc tggcgtagct
     3601 gccccagtca tcgctggtca ccatgccgat acagaacgga gtcaacaggg c
//
LOCUS       DAJTNW010000205         3590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNW010000205 DAJTNW010000000
VERSION     DAJTNW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            32..493
                     /locus_tag="OO179_004096"
     CDS             32..493
                     /locus_tag="OO179_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5533943.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="OO179_004097"
     CDS             complement(534..1004)
                     /locus_tag="OO179_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5533944.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OO179_004098"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OO179_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5533945.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OO179_004099"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OO179_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5533946.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAJTNW010000206         3538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNW010000206 DAJTNW010000000
VERSION     DAJTNW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.212"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..455
                     /gene="pgrR"
                     /locus_tag="OO179_004100"
     CDS             <1..455
                     /gene="pgrR"
                     /locus_tag="OO179_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5533947.1"
                     /translation="EMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAI
                     NLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVER
                     AIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            792..2405
                     /gene="mppA"
                     /locus_tag="OO179_004101"
     CDS             792..2405
                     /gene="mppA"
                     /locus_tag="OO179_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5533948.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OO179_004102"
     CDS             complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OO179_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5533949.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          771 c          814 g          947 t
ORIGIN      
        1 gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg
       61 ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca caattaatag
      121 atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa
      181 tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag
      241 acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg
      301 ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc actccagatt
      361 tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat
      421 ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta
      481 attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt
      541 acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcccgaa
      601 cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt tacactaaat
      661 ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt ttttgatgga
      721 agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta
      781 gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg gtcagcagca
      841 tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc
      901 tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc
      961 cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac gaaaaagggg
     1021 agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc tggactttta
     1081 ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct
     1141 acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg tttgccgcgc
     1201 tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct gatcagcttg
     1261 gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt
     1321 ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg
     1381 gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc
     1441 gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat aacgccaaaa
     1501 cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt
     1561 acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt
     1621 tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat tattatgcct
     1681 ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta agtatgacga
     1741 tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca gcgtggcatt
     1801 ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc
     1861 aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa
     1921 aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg
     1981 ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat
     2041 ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg
     2101 tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg cattcaggaa
     2161 atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa
     2221 ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag
     2281 caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg tggctgaaag
     2341 gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat attgtgaagc
     2401 attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc
     2461 gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt
     2521 ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa
     2581 gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt
     2641 cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt
     2701 tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta
     2761 aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg
     2821 acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa
     2881 ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt
     2941 cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa
     3001 tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag
     3061 aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata atggtgattt
     3121 tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt
     3181 tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca atcagggacg
     3241 tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt tctataacat
     3301 aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat tgcaccgcat
     3361 ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc atcagaatca
     3421 atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag
     3481 cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggc
//
LOCUS       DAJTNW010000207         3528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTNW010000207 DAJTNW010000000
VERSION     DAJTNW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.213"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(104..1813)
                     /locus_tag="OO179_004103"
     CDS             complement(104..1813)
                     /locus_tag="OO179_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5533950.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OO179_004104"
     CDS             complement(2245..2964)
                     /locus_tag="OO179_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5533951.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3089..3528)
                     /locus_tag="OO179_004105"
                     /pseudo
     CDS             complement(3089..3528)
                     /locus_tag="OO179_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1028 a          720 c          611 g         1169 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatt ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgatcatggt gggaaggctt agaacgccat attcagtttc ccatagtgga
     3121 acatcactcc cagcttaaag cgctcccggt tgcggtagcc cttggctttt attctcagca
     3181 gcctgatctt gctgttcagt gcttccgcat ttccgtttga tacgccatgg cgcatggcgt
     3241 tcaggatacc gtacaacctt ttccctatcg ttttagccac attcttcatc atggggacgt
     3301 cactgttggc cgcagccatc tctgccagtc gttgcgtctt tcctcgctcc atggtctgct
     3361 ccagatatcc tttgccagtt ctttcaacgc ccagcacagg ctcgtcagct tcatctgtgc
     3421 acgcagccat atcagctttt cctgcctgga ttcggtcatc catttatcgc tgtgctgcca
     3481 caggaagcga gttccctttg cctgacgacg actttcaaca ggaagatg
//
LOCUS       DAJTNW010000208         3441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTNW010000208 DAJTNW010000000
VERSION     DAJTNW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..354
                     /locus_tag="OO179_004106"
     CDS             <1..354
                     /locus_tag="OO179_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terL-like domain protein"
                     /protein_id="HCR5533952.1"
                     /translation="DEWKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFS
                     EINADRKSENAYARRVNYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEI
                     RLTPLTTENSILWMS"
     gene            342..575
                     /locus_tag="OO179_004107"
     CDS             342..575
                     /locus_tag="OO179_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5533953.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            575..2041
                     /locus_tag="OO179_004108"
     CDS             575..2041
                     /locus_tag="OO179_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5533954.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            1932..2675
                     /locus_tag="OO179_004109"
     CDS             1932..2675
                     /locus_tag="OO179_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5533955.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2679..>3441
                     /locus_tag="OO179_004110"
     CDS             2679..>3441
                     /locus_tag="OO179_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5533956.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPT"
BASE COUNT          878 a          855 c          982 g          726 t
ORIGIN      
        1 gacgaatgga aggccaaaga agatgaatta ctgaagagct gccagcgtac ttatcaggcg
       61 gcgctggagc gtgaagcaga tattgtttac gactctatcg gtgttggtgc gtctgccggt
      121 gctaaattct ctgaaattaa cgctgaccgg aagagcgaga acgcatacgc gcgacgtgtg
      181 aattaccaga ggtttaacgc cggtgctggt gtgcatgagc cagatgacga gtacaacggc
      241 atccccaaca aagacttttt cgcaaatctt aaggctcagg catggtggct gaccgtttca
      301 gaaatacgtt taacgccatt aacaacggag aacagtatcc tgtggatgag ctgatcagca
      361 tagattctcg ttgtccgttg cttgaaaagc tgaaactgga actgacaaca cctcatcgtg
      421 atttcgaccg taacggacgt gtgatggtcg aaagtaaaaa agacctcgca aaacgcgaga
      481 taccgtcacc aaacgttgct gatgcattca ttatggcctt cgcgccaatc gatacatcgc
      541 tggatatctg ggaacagctg gggagacagg cctgatggca cgaaacaaac aagccctgcg
      601 gcgaactgtg caggccacag ctgatggtta tgagaatttt attgcccgag tagggatgca
      661 gacacctaac cagcactcag catccaccta ccgggctaat ttcaccagtc gtaaccgcat
      721 gctggtggaa tggtcctatc gttcatcctg gatcatcggc gaagcagtcg atgctatccc
      781 ggatgatatg acccgcaaag gcattcgcat cacttcggaa attgatgcaa aagatcgtgg
      841 cattctcgaa tcacaactgg atgagttgca aatctgggat gcgctgaatg acgtgctgaa
      901 atggtcgcgc ctctacggcg gcgcggtggg tttcatcatg attgaggggc aggcaccaat
      961 gaccccgctg cgacccgaaa ccatcggtaa gggcaagttt aaggggattc tcccgctcga
     1021 ccgctggatg gtcgacccgg cactgacccg ccgcattaaa gatatggggc cggacctggg
     1081 taaacctgag ttttacgatg tggtgaccac agcaacggga attcctgcct ggcgcattca
     1141 tcacagtcgc ctgattcgct ttgatggcgt cacgttgcca tttcagcaga agatgaccga
     1201 gaacgaatgg ggaatgtcgg ttgtagagcg tatctgggat cgtcttaccg cgttcgacag
     1261 cgctactgtc ggcgcggcgc agctggtcta caaggcgcat ttgcgtacct acagcgtgga
     1321 gaagctacgc gagcttatcg cacttggtgg tcctgcgtat gaagcgttgc tgaagaatat
     1381 cgacctgatt cgacagttcc agagtaatga aggcatgacg ctcatggact cgcgggataa
     1441 gtttgaaacc catcagtaca gcttcagtgg tctggatgac atcctttcgc agtttgcaga
     1501 acagattagt ggcgctgttg gtatcccact ggtgcggttg ttcggacagt ccccgaaagg
     1561 attttctacc ggtgatgcag accttgccaa ctattacgat cgcatcagtt cgttgcagga
     1621 gaggcgttta cgtcttccgg tgcggcggat actggacatc atgcatcgtt cggaacttgg
     1681 caagccgctg ccggacgatt tcacgtttga gtttaacccg ctctggcaaa tgtctgatgt
     1741 cgatcgctca acggtggcgt taaacaccac caacgcaatc agtacggcgc tgggtgatgg
     1801 tctgatgaca ctgaaagccg ctatgactga tttgcgagaa aattctgacg taaccggcat
     1861 aggggcatcc attaccaacg aggacatcga gaatgccgaa gatgaagcgc cgcccggcat
     1921 cggcgaacct gatgacgaac cgcaggagcc gtcaggcgga aatccggtat cgaaccagcc
     1981 tacgcaggat agcgagggcg gtcggagaca tcgtaaatgg tcgctacgat ggttcaaatg
     2041 acagtatcac ggaaattatt gcggcgctgg agcgttacag tgaaatcatc accccctggg
     2101 cgacaaaggt cgcggaaaac tttactgcgg acctaacccg gcagaacgag aaagtttggc
     2161 ggcaacacag caagaacatc agtcgcgagc tccgcaatct tgtggaaagc gctcctgtgg
     2221 gccaggtgat gcaatccatc atcgccgaac aggtcaagta catcaaatct ctgcctctcg
     2281 aggccgcaga cagggtgtac gacatccaga acaaagcgat agaggctgtt gtcaccggtg
     2341 gtcgggcgga gcagtttgct aaagagattg catctaccgg cgatgttgct aagtctaggg
     2401 ccgatctgat tgcccgaacg gaactgggaa gagcaacggg cgcgctggat atggcccgag
     2461 cgatagctat tggctcggat ggttatattt ggcgtacagc cgatgatggc gatgtccgag
     2521 attcccacga tcacatgaaa ggtaaattcg tccgctggga ttcacctcca actttggacg
     2581 gcatgaccgg ccacgcgggc gagctgccaa actgccgctg ctataaagag atcgtgtttg
     2641 ttcgcgttcc attcgcaatg aaaagggcag cataacccat gaaatacttt tttgagacca
     2701 ggctcgggga aacccgatac cgcctggctg acggctcgtt gctgtgcaaa gacgtgccga
     2761 taggacgaac aggtaagcag ctctatggtg ctgatgacct gccaaaactg aaacccgata
     2821 agttcgttga aatagtcgtc acgcgttctc ctgagcaggt attccatccg gccacgcttg
     2881 cctcattcga agggatgagc atcacgatcc tgcatcctga agatgaaaac gggaatgtgc
     2941 ggctggtaaa tcccgagaac tggaaagtgc ttgctgtcgg gcacctccag aatgtccggc
     3001 gcgggacggg tgagcagtct gatttgatgc tggctgacct tatcgtcaaa gacgaaagcg
     3061 ccattcagct tatcgaagat ggcctgcgcg aagtgtcgtg cggctatgac gcggagtacg
     3121 agcagaccga gccatgtaaa gccgagcagg tcgatattac cggaaaccat gtggctcttg
     3181 tccctaaagg cagagccgga aatcgttgtg caattggaga cagagacaca atggcaaatc
     3241 aaaagaaaaa ctggtggaac cgcatgcgtg cggccatcaa gacaggagat gccgacacca
     3301 tgaacgaact ggtggagtcg gctcccgcat cggttacagg agatgagggg gatttgccgc
     3361 agggcgttaa tctcaacatc aacctgtccc cgcagcaacc actaccggac aaagcaccag
     3421 agatgggcgg agatccaacc g
//
LOCUS       DAJTNW010000209         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNW010000209 DAJTNW010000000
VERSION     DAJTNW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="OO179_004111"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="OO179_004111"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5533957.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHW
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="OO179_004112"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="OO179_004112"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5533958.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="OO179_004113"
     tRNA            2656..2731
                     /locus_tag="OO179_004113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3180)
                     /locus_tag="OO179_004114"
     CDS             complement(2746..>3180)
                     /locus_tag="OO179_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5533959.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          753 a          804 c          878 g          745 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 ttggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc attaaaatca ataagttaca catcattagt
     2761 accttcctta ttttttgact gggacaaatt tgggaccgat gggttcagga tcgagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgacaac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgattccc
     3121 gatttgtttt ttcaatacgc gacatgcagt atcattcagc gcaacgccaa ttgcgcggtt
//
LOCUS       DAJTNW010000210         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNW010000210 DAJTNW010000000
VERSION     DAJTNW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO179_004115"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO179_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5533960.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO179_004116"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO179_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaac atcatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctctttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgatggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataattc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTNW010000211         3099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTNW010000211 DAJTNW010000000
VERSION     DAJTNW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.218"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            8..2911
                     /locus_tag="OO179_004117"
     rRNA            8..2911
                     /locus_tag="OO179_004117"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3007..>3099
                     /gene="rrf"
                     /locus_tag="OO179_004118"
     rRNA            3007..>3099
                     /gene="rrf"
                     /locus_tag="OO179_004118"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          815 a          682 c          969 g          633 t
ORIGIN      
        1 gttgtgaggt taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa
       61 ggacgtgcta atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt
      121 tccgaatggg gaaacccagt gtgattcgtc acactatcat taactgaatc cataggttaa
      181 tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa tcaaccgaga
      241 ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag tgtgtgtgtt
      301 agtggaagcg tctggaaagg cgtgcgatac agggtgacag ccccgtacac aaaaatgcac
      361 atactgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat atggggggac
      421 catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg tgagggaaag
      481 gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac gtacaagcag
      541 tgggagcacg cttaggcgtg tgactgcgta ccttttgtat aatgggtcag cgacttatat
      601 tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta actgggcgtt
      661 aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt gaaggttggg
      721 taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg acttgtggct
      781 gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc tatttaggta
      841 gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg tcatcccgac
      901 ttaccaaccc gatgcaaact gcgaataccg gagaatgtta ttacgggaga cacacggcgg
      961 gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg tcccaaagtc
     1021 atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg cttagaagca
     1081 gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc ggaagatgta
     1141 acggggctaa accatgcacc gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg
     1201 agcgttctgt aagcctgtga aggtgtgctg tgaggtatgc tggaggtatc agaagtgcga
     1261 atgctgacat aagtaacgat aaagcgggtg aaaagcccgc tcgccggaag accaagggtt
     1321 cctgtccaac gttaatcggg gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta
     1381 gtcgatggga aacaggttaa tattcctgta cttggtgtta ctgcgaaggg gggacggaga
     1441 aggctatgtt ggccgggcga cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc
     1501 aaatccggaa aatcaaggcc gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa
     1561 tgccctgctt ccaggaaaag cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg
     1621 acacaggtgg tcaggtagag aataccaagg cgcttgagag aactcgggtg aaggaactag
     1681 gcaaaatggt gccgtaactt cgggagaagg cacgctgata cgtaggtgaa gcgacttgct
     1741 cgtggagctg aaatcagtcg aagataccag ctggctgcaa ctgtttatta aaaacacagc
     1801 actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta
     1861 attgatgggg ttagcgcaag cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa
     1921 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg
     1981 cgtaatgatg gccaggctgt ctccacccga gactcagtga aattgaactc gctgtgaaga
     2041 tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc tttactatag cttgacactg
     2101 aacattgagc cttgatgtgt aggataggtg ggaggctttg aagtgtggac gccagtctgc
     2161 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtg gacccgtgat
     2221 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
     2281 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
     2341 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
     2401 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
     2461 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
     2521 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
     2581 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
     2641 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
     2701 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
     2761 tctaagcacg aaacttgccc cgagatgagt tctccctgac cctttaaggg tcctgaagga
     2821 acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa
     2881 ccggtactaa tgaaccgtga ggcttaacct tacaacgccg aagatgtttt ggcggattga
     2941 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     3001 aattagcctg gcggccgtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     3061 aaacgccgta gcgccgatgg tagtgtgggg tctccccat
//
LOCUS       DAJTNW010000212         2953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTNW010000212 DAJTNW010000000
VERSION     DAJTNW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.219"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="OO179_004119"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="OO179_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5533961.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="OO179_004120"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="OO179_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5533962.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>2953)
                     /locus_tag="OO179_004121"
     CDS             complement(2711..>2953)
                     /locus_tag="OO179_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5533963.1"
                     /translation="GTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHP
                     HEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          687 a          653 c          734 g          879 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcc
//
LOCUS       DAJTNW010000213         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTNW010000213 DAJTNW010000000
VERSION     DAJTNW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.220"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO179_004122"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO179_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5533964.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO179_004123"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO179_004123"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5533965.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO179_004124"
     CDS             2603..>2951
                     /locus_tag="OO179_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5533966.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTNW010000214         2835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTNW010000214 DAJTNW010000000
VERSION     DAJTNW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.221"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..180
                     /locus_tag="OO179_004125"
     CDS             <1..180
                     /locus_tag="OO179_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5533967.1"
                     /translation="YPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEV
                     LALRLPENGSQLHHS"
     gene            complement(360..983)
                     /locus_tag="OO179_004126"
     CDS             complement(360..983)
                     /locus_tag="OO179_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5533968.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(934..2349)
                     /locus_tag="OO179_004127"
     CDS             complement(934..2349)
                     /locus_tag="OO179_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5533969.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NGPIYHTRYRYWPISRLTDWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQG
                     EADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCA
                     LSGVPDAHVSGAMPGLFIRTS"
     gene            complement(2413..>2835)
                     /locus_tag="OO179_004128"
     CDS             complement(2413..>2835)
                     /locus_tag="OO179_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5533970.1"
                     /translation="TDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGL
                     SVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWG
                     AGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          592 a          867 c          700 g          676 t
ORIGIN      
        1 taccctcaga gggtggctga ccggctgaaa gcatcaggtc tgagcggtga tgcggatgcg
       61 gagagggaag ccggtgcaca ggtgatgcgt gagactgaac agcagattta ccgtcagctg
      121 actgacgagg tactggccct gcgattgcct gaaaacggct cacaactgca ccattcataa
      181 tcacatcgca taaaccacag accggactga ctccggaaaa acagaggccc gcccccgggc
      241 ctccccggat tcatccgttt ctctgttcag cctgaccgca caccccggcg gccggatgac
      301 agactccgcc tcggtaagca aagcggtctt ctgtgattcc gccagttgcg gcttattcat
      361 tacttaacgt caaacgcccg aattgaagcc aaatcatcca gactgctcag ctcctctttc
      421 atctcccgct gacggcgata aatctcatcg ttgcgatcga cctgcgcctg caccattgct
      481 gccgccagtt cttccagttc cggcatcgac agtttcacct gctgattatc cgcatcactc
      541 cacaccatat gtgtttgtgc cgcgacagat tttgccagca tgaccaccgg ggacaggcgg
      601 gcaactgacg aggcgtcagc gttccacgtg cggccgttcc attcaaacgt gaacggcttc
      661 gcctcctgtt ctgtgcgcca tgcttcaatt tcctgacgtc tggcctctct ggccgcttcc
      721 agcatttctg gtgtcacagt gaatggggct atctcacccc atttgccact ttgcagttcc
      781 tgccagattt gctgacccgt cggtgcaaca tcatcagcgg tggctgtgta gggaactgcc
      841 tggtccctgt cgtcaaaaaa aacgtcacag tctactgcgc cactttcggt ataacgggga
      901 ttaatgattt ttttaatttc cacggtgcat tcctcacgat gtgcgaataa aaagcccggg
      961 cattgcgcca gagacatgag catccggcac cccggacagg gcgcaatatg accctggtaa
     1021 tgaatgctct gaacatcccg taatgaaaaa ttgtggggat gctatatacg ttccggtggg
     1081 agtactgggc actgaaatcc ccaccggtcc cagtcgtgag cctctgtatg actgccccct
     1141 gacaagtctg atgactttat caccgtcagc ttctccctgg tacgcagcaa taatcagccc
     1201 gccaatgtca gggtctcccc atctgttgcg gacagagctc gccacgattc tgtaaataat
     1261 atcttctgtg gttatattta ttttcaccca gtcagtcagt ctggatatgg gccagtagcg
     1321 gtagcgggtg tgataaatgg ggccgttaat gccgtaaaaa gtaagggatt tggctctgta
     1381 ccgcggttct gttgtctcag ggcgtgcatc agtccaccgg atgctgagca ccccttcaaa
     1441 ccgtgtgtcg ggtatgatga tgccgtaggg gccagcaacg gaatattcac ctggcagcgc
     1501 attccttacc caggccagga aatcactctt agtgtcaaaa cggataacat cttcaggcag
     1561 aaaagcacac ccaaagccga atgcgccggg tatcgccaga cggccttttg tccggtcgta
     1621 aatgtcgctc tgtgcttcca tcgtggccgc acttttcagc cccagattat cccgggactt
     1681 ctgttgtgcc ttttcgcctg ctgctgcgat ttctgacaga tggttagcca ttttcagagt
     1741 gccggtcagc gcagcatcaa tgtcattttt ggcctgttct gctgcgcggg cataacctgc
     1801 ggcatcctgt gcgctctgtc cggctgcggc tgcatttgct cctgtgcttg tcacatcttc
     1861 cgcagtggcc tttctgtcct cagctgtgac ggttgcatcc tgtcgggctt tctccgcaaa
     1921 acgttgtgca tcatcacggg ccgttgcggc tgccgccaca tcctgcgccg tctgctgtgc
     1981 gtttccggct gcagccccgg cactctgctg cgcctgcgcc accatttcct caaagcgctt
     2041 catcacctcc ggacgtaaat caccatcctt tggtgcattc agaaacgcgt tcagtgtccc
     2101 cggtgcatca gtcggtgcca catcaatatc cccgacacgg gttggctgcc agccgttaca
     2161 gtgaagcgca acctcgtaat accccggctc agcctcaatc acataagcac cgttattgtc
     2221 cgtcacgcaa gtggcaacaa cgtgtgccac aacggtcgga ctggttcttc tggcccgcag
     2281 ttcaatcgca caatttacga caggtttacc cgccccatct ttcagtacac ctgaaatctt
     2341 tactgccata ttcaccccac aaaaagcccg cctgaaccag cgggctgtca taacactgtg
     2401 ttacctggct aatcagaact tataaccgac acccacgatg aaaccgtcag tgcgccagtc
     2461 gccactgccg gaaccttcat aagcaaggtc aatggccacg gattcggtcg ggttaaactg
     2521 cacgccagcc ccccacgcca gagacgtgtt gctgtggcgg ttgtcatcac tcccggtcag
     2581 cacatcgtgc gttttcccct tgttgtcagt tacgcggaga taatccccgg agaaggtcga
     2641 cacacggctg taagccacgc ccgccatcgc atacgcgctg aaccattcat tcacgcgcac
     2701 agacagcccc gccatcacgc tgaaccagcg gttacgcacg gaatcctcat gccagcgggt
     2761 atcactgtaa tgcgtttttt gctcatcttc agcgttggca tagctgaatg acgtcaccag
     2821 ccccagcgtg tccgt
//
LOCUS       DAJTNW010000215         2801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNW010000215 DAJTNW010000000
VERSION     DAJTNW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..258
                     /locus_tag="OO179_004129"
     CDS             <1..258
                     /locus_tag="OO179_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5533971.1"
                     /translation="AQVNRDSGTLRGRRTIFGDCAGEEQLCTWRRLRPPRFNLVIKAF
                     YMRLLAAGNAKKVALVACMRKLLTIMNAMLRKNEEWNESYL"
     gene            842..1558
                     /locus_tag="OO179_004130"
     CDS             842..1558
                     /locus_tag="OO179_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533972.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2110..2514
                     /locus_tag="OO179_004131"
     CDS             2110..2514
                     /locus_tag="OO179_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5533973.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2577..2801
                     /locus_tag="OO179_004132"
                     /pseudo
     CDS             2577..2801
                     /locus_tag="OO179_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          803 a          547 c          617 g          834 t
ORIGIN      
        1 gctcaggtaa accgggactc gggaactctg cgcggtcgac gaaccatttt cggtgattgt
       61 gctggggaag aacagctctg tacatggcgg cgcttgaggc ccccccggtt taatctggtg
      121 ataaaggcgt tttacatgcg cctacttgca gccggaaacg ccaaaaaagt ggcactggtt
      181 gcctgtatgc gtaagttgct gaccattatg aacgcaatgc tcagaaaaaa cgaagaatgg
      241 aatgaatcgt atttatgaaa actggattgt gggccataaa tattccaaaa agatagcccg
      301 ctccgtttat taccggaacg ggcgagagca tgacattgtc tgttattata atgagactgc
      361 tgattctact tatttttaat ttcaagaaaa gcgttttcta caaagaatta tgagatttaa
      421 tgttttgttt agacaatatt atcattctgt tcaataatga aagtcttcat caacaacaag
      481 taaaaacaac gtccctctca tcgaaccgga cttgcagatt tcccgcatcc ggctctcttg
      541 caacacatca gaccttcacg ttcgggcggt tatggggaag gtccagcaag ctgtatgagc
      601 cctaactgtt tgaataccag tagattacca gaaacttcta tcgattgaag acattaccaa
      661 ctagcgttac taaatgttga ccaagttttg ttcacttgtc tggttttgat agtagcattc
      721 tgcagagtat tttgatatga taaatatgca ttaacaatga tattattgtt aatttcctag
      781 catgaaaatt aaaagatcaa cttttatctc gaatattttt tacattatca gttggtttct
      841 tatgaatgac aattcattgc ttagaaattc ttcattattc atcgcctata tgggatgtgt
      901 agggtgggtc agtgcttatt cttatggctg gggaacctct ttttactatg gatttccatg
      961 gtgggttgtt ggtgcaggtt tggatgatgt tgccagaagt ttactctatg ctatcatagt
     1021 aatgggtata ctttttaccg gttgggggat tggtatttta ttttttcttc ttataaagaa
     1081 aagaagtaaa atacaagact tgagtttttt tagactattt tttgctatta ccctactgtt
     1141 tttccctgta atattcgaac ttttaattct caaacaatat ttcatacttc cattatcact
     1201 ttcattcatt attagttcat tggttatttc aataataatt cgtatatatg gacggatctt
     1261 ttctgtatca tgtttttcag atatcccttt tgttagagaa caccgcatta agttaattat
     1321 ggctggtttt ttggtttatt tttggttttt ttcctttctt gttggctggt ataaacctca
     1381 attaaaaaaa gaatatcaga tgctttgtta taataatagt tggtattatg ttcttgctcg
     1441 ttatgattca cggctcgttt tatctagttc atttaaagat gacagtaaca gattcttaat
     1501 atttaataca gaacagtcgg gattttatga aatcaatgat gtttatgtca gaaagtaata
     1561 atcctctccc acaaagtgaa ttaaatgaat atataatgcg ttcgaagtat atgtattgag
     1621 ctgtcatact gagaaacaaa ccttagggca gcacaattaa agccagaaac aactatgcaa
     1681 gaaagttact aaataagtgc tcggaacagt aaaacactca ggggggactc agtccggatt
     1741 atgtaatcag ataaattgcc aaacatcaca aatcaccagc cggactgagt gatgccgagc
     1801 attgcaccaa taccccgtga tgaacgatgc ctgatgcaga aagctcccta taaaacaaag
     1861 aacaaaaact acgaccggcg attcaccgtc atactgatgc tgcatcgtgg tgacactgtc
     1921 tgtcactttc atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc cggcgggatt
     1981 tgattgtccg gcgtctagct tccacttccc tgatggaact gaaagttgtt atggccggtc
     2041 agcttgccct gtccgcagga catgaggtgc tgtgacggac agcaccagta acggaaagtg
     2101 acaatgaaaa tgaatactgc gctgatagtt gcactaatgt gtatgtggta tgcggtaccc
     2161 gctgctgcaa aagagacttt actggctatg ccacgtaatt caacagaaca ttgctatgcg
     2221 gagattaatg tgcacggacc atatggggtg tatttccgag tggtcccgca tccccccggg
     2281 ggaaagagct gggtcgaatg taacagtgac tactattact ctgataaacc accgggagta
     2341 cagatactcg gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac cacctcgaca
     2401 ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc acgtgggatg
     2461 ataattcaca ggatacggaa acgtcctgta gtggctgtaa gcgatgagat gtgatcttac
     2521 ccagcaatag tagacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga
     2581 caaaaacagt atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa ttttgcagtg
     2641 aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc agcccgtgaa ctcagcctgt
     2701 atgaatctca gctctatgcg tggcgcagca aactgcagca acagatgacc tcgtctgagc
     2761 gtgaaagtga actgcccgcc tgaagcggca actggcggag c
//
LOCUS       DAJTNW010000216         2721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNW010000216 DAJTNW010000000
VERSION     DAJTNW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.223"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            6..81
                     /locus_tag="OO179_004133"
     tRNA            6..81
                     /locus_tag="OO179_004133"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO179_004134"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO179_004134"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO179_004135"
     CDS             complement(299..1057)
                     /locus_tag="OO179_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5533974.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO179_004136"
     CDS             complement(1065..2168)
                     /locus_tag="OO179_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5533975.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2721)
                     /locus_tag="OO179_004137"
     CDS             complement(2178..>2721)
                     /locus_tag="OO179_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5533976.1"
                     /translation="RRSWPIAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVL
                     IPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQAL
                     RVIIPPLTSQYLNIVKNSSLATAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLII
                     SLTISLLMNIYNRRIAIVER"
BASE COUNT          732 a          722 c          658 g          609 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg c
//
LOCUS       DAJTNW010000217         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNW010000217 DAJTNW010000000
VERSION     DAJTNW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            230..769
                     /locus_tag="OO179_004138"
     CDS             230..769
                     /locus_tag="OO179_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5533977.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO179_004139"
     CDS             complement(995..1318)
                     /locus_tag="OO179_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5533978.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO179_004140"
     CDS             1604..1798
                     /locus_tag="OO179_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5533979.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTNW010000218         2480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNW010000218 DAJTNW010000000
VERSION     DAJTNW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..566
                     /locus_tag="OO179_004141"
     CDS             <1..566
                     /locus_tag="OO179_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5533980.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            524..1201
                     /gene="bglJ"
                     /locus_tag="OO179_004142"
     CDS             524..1201
                     /gene="bglJ"
                     /locus_tag="OO179_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5533981.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="OO179_004143"
     CDS             complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="OO179_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5533982.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2168..2404
                     /locus_tag="OO179_004144"
     CDS             2168..2404
                     /locus_tag="OO179_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5533983.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          635 a          546 c          642 g          657 t
ORIGIN      
        1 tagtcattgc tgatttagcc ggtgaaagtg aagatccacg ttctgtttgt gaacattatt
       61 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      121 attcccaggc tgtagaactg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      181 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcacgcagag cgtatcagcg
      241 ccatgctgac ctctccggca atgactgaaa ctcatgattt tagctatcgc tccgtcattc
      301 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      361 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      421 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      481 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      541 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
      601 tttatccaga aagtttgcgg gcaaccctta taagctccat acctatacca gtcaggagtc
      661 atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
      721 gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta agtttccgcg
      781 tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg gttcattgtc
      841 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
      901 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
      961 cattaaccaa agccggacgt taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1021 tggctactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa cgatccgagc
     1081 gcataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1141 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1201 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatagcca
     1261 cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac cagcaggccg
     1321 tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cgttcgtgag cgttttctca
     1381 aaaaagaggg catggcgcag cgattcaaca gtagcctcgc cgagcagttg cttcatctca
     1441 gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1501 ccggtcgctt ctagtgcttg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1561 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     1621 gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac atctaatgct
     1681 ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat ataccattgt
     1741 gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt gcgatagata
     1801 tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     1861 gtcatggcgt taagcggagc gggttcatcc aggcggataa actccagcaa atgttcacga
     1921 tgtttggcga tcatcgcccg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     1981 gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat cgtgatagta
     2041 atctaaatga taatgattgc taatcctagc gataggttta ctcgatagca agggatttct
     2101 ctggcttgca aatgataaaa attatcatat gatattggtt ttcattatca atgaaagaga
     2161 tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc cgggattgct
     2221 gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg tcattctgat
     2281 gggattagta ttgacgccgg tgatgctgta tcacaaacag ttgcggcatt acattttgct
     2341 accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt tgaatcaggg
     2401 atgacaaaac agggaagaaa caagaaagga agtaaagata attggtgcga ggggggggac
     2461 ttgaaccccc acgtccgtaa
//
LOCUS       DAJTNW010000219         2440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNW010000219 DAJTNW010000000
VERSION     DAJTNW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            82..705
                     /locus_tag="OO179_004145"
     CDS             82..705
                     /locus_tag="OO179_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5533984.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(759..1931)
                     /locus_tag="OO179_004146"
     CDS             complement(759..1931)
                     /locus_tag="OO179_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5533985.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2280..>2440)
                     /locus_tag="OO179_004147"
     CDS             complement(2280..>2440)
                     /locus_tag="OO179_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5533986.1"
                     /translation="EMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSR
                     NKEGTNDV"
BASE COUNT          738 a          444 c          504 g          754 t
ORIGIN      
        1 tgtgaaggaa atggtactca gggggattcc agtacaggtg gtgcgcaatt agctataaat
       61 ggccttaacg gttcgtgcgc tttggtatta aacaacccat actttgaggc taatgccggg
      121 ggggcagacc ttgctataga taatactggg acgcgccctg taactgtggt tataaacggt
      181 ggaaattttc atcgcgtgag cagtgcaaga tacacgcaca cgaatattca agtaacaagc
      241 tcaggtgggg gaaaagttac agtcatactc aacggcacaa cgttccagag cgcaggggat
      301 tatcagccaa gtgcagaacg accatactgg ataacaggag ctaattgcga ggtagttgac
      361 attgggtgtg tttttacaga aacaaccagt aaggtaacat cggcttctgc attgtcagtc
      421 acacgctcgg gaaaaattaa cgccaatggt agtatcgacg ttgctactgg tgtttcatcg
      481 gttaacgtgg tttctacagg tgtgtatgat gttagctttt ctcatccgct cgcagcagtt
      541 gctagcggtt atattgtgca gataacacct atatctgcgc ctgattcagt tagttgtgat
      601 gttacttata ttggcgtaga tacatttagg gtgaccctgc gcaatacgct tagcggggca
      661 ggaatctcca gttcgtttgc atttagcatt accagactaa tataaataca ttcgccgccc
      721 catttggaca tatatgtagg gcggcttatg tttattgttt attgattgtt gtttgttaat
      781 gtgggtatta attcttttac taaagttgtg gtttgttttg ttaatttgat gaatggcgtc
      841 tctattaatt taaaagttag gcatgatgtg aacgtaataa gaaatgcagt tgtgcttacc
      901 agtatgataa atgtattttc agtgtaatta ttaggaataa tccacgtcat taggcaataa
      961 aggaatatcc catgcagaag gtaaacgctg taggtagttt ctcctagctt tctggttata
     1021 tttaatctta atattccata caaatcacaa cctgatgcga taatgataaa tgttattcca
     1081 caaagaatca gtggtagcgg tgcgtaagtt gttttgaaat acgtcatttc aaaaaccatt
     1141 atcgcagtta ttataattgg tgttactttt gctttggcaa ttccattgac tatctttgat
     1201 ttatttagca aaaatgccag gagcccaaaa agaaatgatg ctatgtgtat tttactgaaa
     1261 aataaaatga aaacaaatag cgatattgcg ctaatcataa ccatgtaaat actgactttt
     1321 ctttttatga gcgcggaaat taccggaaga gaaaaataaa agaaccattc atatacaagt
     1381 gtccatgtta cagcggcatt gatagtaaat gaatctttta cgtcattaat gttcggcata
     1441 cctagcgcag tgaatggcaa ccacttcatt attgacacaa aaagctcttc tgtggatacc
     1501 tgcattctcc atccagactt aaaacctaca atgataaaaa tgagacatag gctaactatg
     1561 aacattgggg ttaatcgtag taatcttgat acataaagcc ttgtccagtc ctgatctcca
     1621 gagataatct ttgaaaagaa cagataacca gtaatcataa aaaagaatga cacaccaact
     1681 tggcctaaat ttgctaacag atttgatgaa ggtgcttccc atactccaga tgacaagtat
     1741 ccattccaaa ttgctgcgtg atgaataagc acgaaaattg ctaagaaccc ccgcattcca
     1801 tctatttgat tattcctgcc gtgctcaaca agatttattg gggaaatttt atcaaataac
     1861 cccacagcta ataacgacat caatatcacg gatgttatta ataataaact gttaatttca
     1921 atcatatgca tgctctatta cgcaattaaa atttctccaa gtatactgac ttttagttaa
     1981 tgatcatata gttatgtgta actgatgaaa caaaacggag acacaccaag ctttgcactg
     2041 gattgcaagg ctttgtgctc ttctgtgaat gtgtggctat atgtttgaag atcgttgtgc
     2101 cgtatttgtg acatatatat ggcaacatca tgcatcaact ttctgtttgt gtcatcaact
     2161 atagcttagt gaatgcggtt aatgcttgct aaaacagata gttatgattg gtgctacaga
     2221 ttcgtaatgc gaaggtcgta ggttcgactg ctattatcgg caccatttaa atcaataagt
     2281 tacacatcat tagtaccttc cttatttctt gactgggaca aatttgggac cgatgggttc
     2341 agaatcaagt ctatttgccg tgcgtgttcg gtaaggtgat taggtgcgag gtgagcatat
     2401 cgacgaacca tttcgataga ctcccagcct cccatttcct
//
LOCUS       DAJTNW010000220         2432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNW010000220 DAJTNW010000000
VERSION     DAJTNW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            65..1543
                     /locus_tag="OO179_004148"
                     /pseudo
     CDS             65..1543
                     /locus_tag="OO179_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="OO179_004149"
     CDS             1540..>2432
                     /locus_tag="OO179_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5533987.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DAJTNW010000221         2380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNW010000221 DAJTNW010000000
VERSION     DAJTNW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..97
                     /locus_tag="OO179_004150"
     CDS             <1..97
                     /locus_tag="OO179_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5533988.1"
                     /translation="LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            90..1049
                     /gene="ssuA"
                     /locus_tag="OO179_004151"
     CDS             90..1049
                     /gene="ssuA"
                     /locus_tag="OO179_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5533989.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1046..2191
                     /gene="ssuD"
                     /locus_tag="OO179_004152"
     CDS             1046..2191
                     /gene="ssuD"
                     /locus_tag="OO179_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5533990.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2200..>2380
                     /locus_tag="OO179_004153"
     CDS             2200..>2380
                     /locus_tag="OO179_004153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5533991.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          523 a          649 c          672 g          536 t
ORIGIN      
        1 tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg ttcaggttcc
       61 tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg gcgctggcgg
      121 gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa gcgttacgta
      181 taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag ttactggaaa
      241 aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg caaatgttgg
      301 aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg ccaatctttg
      361 cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag cccaaagccg
      421 aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt aaaggtcaca
      481 aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca ctgcgtcagg
      541 ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat gcccgcgccg
      601 cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac tccgctgcgt
      661 tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa actggatcgt
      721 tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag ggcgtactgg
      781 caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc atcgctttac
      841 tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat catcgccctc
      901 ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa acggcagatc
      961 tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc atctggcagc
     1021 ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt taccgaccca
     1081 cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc acggttatct
     1141 gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga tcccaacggg
     1201 gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga cgcagcggct
     1261 gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg cccgccaggc
     1321 cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca caggcagcga
     1381 tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct acgaagcctc
     1441 ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca atttcaacgg
     1501 taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac agccttatcc
     1561 tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag aacaagttga
     1621 tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg aacaagtgcg
     1681 ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc atgtgattgt
     1741 tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc atcttgatga
     1801 tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag gacaacagcg
     1861 aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca atttatgggc
     1921 gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg gtcctacggt
     1981 cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc tttcgggcta
     2041 cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc tggatgtcgc
     2101 cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg tggcaaatga
     2161 ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac gccagtgaag
     2221 aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc ggtgtggcaa
     2281 ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga aggagtggtg
     2341 acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DAJTNW010000222         2373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNW010000222 DAJTNW010000000
VERSION     DAJTNW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..823)
                     /locus_tag="OO179_004154"
     CDS             complement(<1..823)
                     /locus_tag="OO179_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5533992.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="OO179_004155"
     CDS             complement(1424..1672)
                     /locus_tag="OO179_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5533993.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="OO179_004156"
     CDS             complement(2027..>2373)
                     /locus_tag="OO179_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5533994.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          577 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaaaacgc ataaaaatcc
     1681 ctctgcactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg tgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAJTNW010000223         2360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNW010000223 DAJTNW010000000
VERSION     DAJTNW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..173
                     /locus_tag="OO179_004157"
     CDS             <1..173
                     /locus_tag="OO179_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5533995.1"
                     /translation="RCSSVNTLEKIIHKNRDSLSNSERESFNSAADHRLAELITGKLY
                     DRIPKEIWKYVR"
     gene            212..802
                     /locus_tag="OO179_004158"
     CDS             212..802
                     /locus_tag="OO179_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5533996.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1042..1302
                     /gene="repA"
                     /locus_tag="OO179_004159"
     CDS             1042..1302
                     /gene="repA"
                     /locus_tag="OO179_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5533997.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1526..1600
                     /gene="tap"
                     /locus_tag="OO179_004160"
     CDS             1526..1600
                     /gene="tap"
                     /locus_tag="OO179_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5533998.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1593..>2360
                     /gene="repA"
                     /locus_tag="OO179_004161"
     CDS             1593..>2360
                     /gene="repA"
                     /locus_tag="OO179_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5533999.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREA"
BASE COUNT          625 a          539 c          626 g          570 t
ORIGIN      
        1 ggcgatgttc atcagtcaat acgctggaaa aaattattca taaaaacaga gacagtttat
       61 caaacagtga acgtgaatca tttaattcag cagcagacca ccggctagcg gaattaatca
      121 ccggaaagtt atatgacagg ataccaaaag agatatggaa atatgtaagg taataattca
      181 gcaataatta attgccagga gcagataaat aatgaagttg attattttta ttctcattgt
      241 tctgattatt gcagcgctac ttatccggat tatcctcaga agtgtaaatc agcattctcc
      301 tttgctgatg caattgcatg cggcaggtat acggactggt gatgcagagc gaatactgtc
      361 cagcggtgaa tactggcaac gtcagaagac gctgctgaca gaaagggaag tcagttttat
      421 gaaaggactg ttcagaattg tggatatgaa gcggtggtat ctgtgtccgc aggtacgggt
      481 agcggatatc gtccagctga acgggaatat ccggccacga tcacgccagt ggtggcagtt
      541 attcaggatg gtgtctcagt ggcatgttga tgtggttatc gttgagctgc gttcgttcag
      601 tattgttgca gcagtagagc tggatgatgc cagccattta cgaccggaac gcagacgccg
      661 ggatattctt ctggaagagg ttctgaggca ggctggtatt ccgttgctca gaagccacga
      721 tgccagaaaa ctgctgcaga tgaccggaga atggctgaat acaacagggg ctgatcagca
      781 gtcaccggaa catcgtagct gacgccttcg cgttgctcag ttgtccaacc ccggaaacgg
      841 gaaaaagcaa gttttcctcg ctcccggcgt ttccataact gaaaaccata ctatttcaca
      901 gtttaaatca cattaaacga cagtaatccc cgttgatttg tgcgccaaca cagatcttcg
      961 ccacaattct caagtcgctg atttcaaaaa actgtagtat cctctgcgaa acgatccctg
     1021 tttgagtatt gaggaggcga gatgtcgcag acagaaaatg cagtgacttc ctcattgagt
     1081 caaaagcggt ttgtgcgcag aggtaagcct atgactgact ctgagaaaca aatggccgct
     1141 gttgcaagaa aacgtcttac acacaaagag ataaaagttt ttgtcaaaaa tcctctgaaa
     1201 gatctcatgg ttgagtactg cgagagagag gggataacac aggctcagtt cgttgagaaa
     1261 atcatcaaag atgaactgca gagactggat atactaaagt aaagacttta ctttgtggcg
     1321 tagcatgcta gattactgat cgtttaagga attttatggc tggccacgcc ataaggtggc
     1381 agggaactgg ttctgatgtg gatgtacagg agccagaaaa gcaaaaaccc cgataatctt
     1441 ctttaacttt ggcgagtcag aaagattacc ggggcccact taaaccgtat agccaacaat
     1501 caagctatgc ggggagtata gttatatgcc cggaaaagtt caagacttct ttctgtgctc
     1561 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acttattacc
     1621 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgcccgg acgcttccgt
     1681 tctgcgggaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     1741 atgtggcaca tgcccgttcg cttggtctgc gtcggcgcat gccaccggta ctacgtcgcc
     1801 gggccattga tgcgctgctg caggggctgt gttttcacta tgacccactg gccaaccgcg
     1861 tccagtgttc catcaccacg ctggccattg agtgcggact ggcgacggag tctgctgccg
     1921 gaaaactctc catcacccgg gccacccggg ccctgacgtt ccttgcagag ctgggactga
     1981 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gatatcacgt
     2041 tcacatctgc actgtttgct gccctcgatg tatcagagga ggcagtggcc gccgcgcgcc
     2101 gcagtcgtgt ggaatgggaa aacagacagc gcaaaaagca ggggctggat accctgggta
     2161 tggatgaact gatggcgaaa gcctggcgtt ttgttcgtga gcgtttccgc agttaccaga
     2221 cagagcttaa gtcccgtgga atgaagcgtg cccgtgcgcg tcgtgatgcg gacaggcagc
     2281 gtcaggatat tgtcaccctg gtgaaacggc aactgacgcg tgaaatctcg gaagggcgct
     2341 tcactgccag tcgtgaggcg
//
LOCUS       DAJTNW010000224         2357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNW010000224 DAJTNW010000000
VERSION     DAJTNW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(216..311)
                     /locus_tag="OO179_004162"
                     /pseudo
     CDS             complement(216..311)
                     /locus_tag="OO179_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(347..652)
                     /gene="ccdB"
                     /locus_tag="OO179_004163"
     CDS             complement(347..652)
                     /gene="ccdB"
                     /locus_tag="OO179_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5534000.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(654..872)
                     /gene="ccdA"
                     /locus_tag="OO179_004164"
     CDS             complement(654..872)
                     /gene="ccdA"
                     /locus_tag="OO179_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5534001.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1408..2357)
                     /locus_tag="OO179_004165"
                     /pseudo
     CDS             complement(1408..2357)
                     /locus_tag="OO179_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          558 a          630 c          567 g          602 t
ORIGIN      
        1 aaataagtag gagtttttat gaagttcatt ccatgaaatg aacttcagca cttttttatt
       61 ataataagga taaacaaaat tggctgtaaa aaaacgatac tgtttcagtt aaccgtgaat
      121 ggcctgtttg caccgtattg ttcttgtttt tcggttagtt aaacatcgta cttcatattt
      181 gaacggtaag cggctcgtca gaaccgtatt gatatttact gagaggtcag atcaactttc
      241 caaggcaaca gatcgcgtac ccggtttgcc ggccagtcct ggatatgctc aatgacgtaa
      301 cgcagccatt ttgtcaacgc cacgattttt gaccctagcc tgacatttat attccccaga
      361 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
      421 ccccgataac gaagatcggc acactggcca tatcagtggt catcatgcgc cagctttcat
      481 ccccgacatg caccaccggg taaagctcac gggagacttt atctgacagc aggcgggcac
      541 tggccagggg gatcaccatc cgtcgtccgg gcgtgtcaat aatgtcactc tgtacatcca
      601 caaacagacg ataacgactc tctcttttat aggcgtaaac cttaaactgc atttcaccag
      661 tccctgttct catcggcaaa agagccgttc atttcaataa aacgggcgat ctcagccatc
      721 ccctgctgat tttcagcctg ccagcgttcc gcacgcagac ggcgggcctc tttttgcatg
      781 gccgtattta ccaggcctga gatattcacg tttgcggatt taagtaactg atagctgtca
      841 ctgtctatag tgacagtaat acgctgcttc atgataagcc ctcaaaaaca tacttatgat
      901 atgtatatga gtatatatta atggtacaaa ccatcaatat ccataaaaag tacaccatta
      961 attcgctata agtttttaat acaatttttt ctgctgcgat ttttttatac tataattagc
     1021 atacttataa caccgtaagt aaattacatg aaagcaggca cagcgtgatc cgatttttct
     1081 gcgtataacg attttccgga agtgtaatac ggttgcgccc cgggccgggc acccttttct
     1141 gttgttgtgt acgggcccac agagaatggt accggaggcg ggctcagaag tactgagtcg
     1201 ggtgatcgcg tcaggccgcg aagtactgac acgggtattg gtgactggtg atgaagtact
     1261 gccccgggta tgatttcagg ctgattcagg ttctgattct ctgtcagtcc gcgtggttgc
     1321 tggcctggct gatttcctgt tgtcaggcgt tcaacaaccg actgcaggtt atagcccggt
     1381 tcacgaacac ctgacggcag gtatcactca cccggtgaag gtaaatccct caagaggcag
     1441 caactctgcc agtcgctcct ccggccattc cggcagacgc gtcaggacgt ccgtcagcca
     1501 ggcatgtggc tccagaccat tacgttttgc tgtttccaga aggctcatta tctgtgccgc
     1561 ccggtttccg gccatcagtg aaccggcgaa cagccacgct ttccgtccca taacaaccgg
     1621 gcgaatggca cgctcgcaga tattgttgtc caggggcacc gctccgtcac ccaggaagca
     1681 gctcagttca tcgcgccggt tcagaatata gttaatcgcc ttatttaatg gcccgtcggg
     1741 cggacagcac ggctcctgct cttcaagcca ggcaaacagg tcattcacta tcggccggga
     1801 gtaccgttat cgccactgcc gtattttttc cacagggcgc tcacggatga gtttctcaat
     1861 gcggtaaaga cccgcgattt ttctcagctc cacgcctgca cggggctccc tgctggcctt
     1921 atacagattt gcgaagccgc gccctgcgtg actccagcaa caggctgatg tgatgccggg
     1981 gtgattatcc gccagagatt tgtacacgct gtatccgtca ctcaccagcg tactgccaca
     2041 ccagcactgc agccaggtct cagggtatct gagggcccgt ccggtctgcg agtcaaaaca
     2101 gacgaccggc ggaccgcttc tttcaccgct gacgtaagca cacagccagc cggagcagga
     2161 tttaccgcct ttccgggtat caaggagcct cagggacgtt tcatctgcat gaataacatc
     2221 ccgtgtcaga agctcatggc gtagcagttt cgccagcggg gccagtgcag ctccggcaac
     2281 accgaccata tccgccattg tactgacggg gagctccaca cccattcggg agaaaatgtg
     2341 ctgctggcga tacaggg
//
LOCUS       DAJTNW010000225         2180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNW010000225 DAJTNW010000000
VERSION     DAJTNW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..700
                     /locus_tag="OO179_004166"
     CDS             <1..700
                     /locus_tag="OO179_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534002.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            702..1307
                     /locus_tag="OO179_004167"
     CDS             702..1307
                     /locus_tag="OO179_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5534003.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1294..>2180
                     /locus_tag="OO179_004168"
     CDS             1294..>2180
                     /locus_tag="OO179_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5534004.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANS"
BASE COUNT          531 a          507 c          608 g          534 t
ORIGIN      
        1 cgcccgtgcc ggtgtgccgg gcagcgatga ggaaacccgt gcggactttg aaaaccgccg
       61 acgtaattca gttgcccgta atgcccggaa tattctggaa gccatccggg gtgaaatact
      121 ctccacggta gaaaacgtgg tggatgttta cgtcacccat aatccgaaaa aaacagaaca
      181 aaaagccgga gtcagtcagt atccattaac acccggttcg ctttatgttg gcgtgtacgg
      241 cggcagcccg gcagatatcg cggcggccat ctggcgtaag gctccgccgg gtattgatat
      301 gaatggcaac acaacgttca ctgttgcaga taaggagtac gatccgccgt atcctgaata
      361 cgtgatcagc tggcagacac tcaaacccgt cagtctgcat gtcagtgtga cgctgaaaaa
      421 aagtgactac ctgccttcag atattacccg acaggtacag caatccgtgc tggacgcatt
      481 taatggtaca gatggtggtc tgcgggcaag agttgcctct gttgtctctg ccgggcgcta
      541 ctatgccggc atttacaaaa ccgatccgga acatattgat attctgggcc ttactgtgag
      601 ccgtgatggt tcgtcatgga caacggctgt cactttcggg atagatgaga ttccggttct
      661 ggatgtgtcg gatatcagtg tgaaactcca ggaggcataa cgtgcagaat gtggctgcca
      721 ctgtgcttgc gcagtatgct gccagccccc gactcaatgc cctcattaac agctttaacg
      781 cagcgctttc ccccgacagt tttatcagtg atttttatgg tcttatctgg aacatcgata
      841 ccgcagaaaa gtatggtctt gatgtctggg gaaagattgt gggtgtcagt tgctggctga
      901 cggtaaagga cgattttaat tacctgggct tcagcgagtc caggatggac accccggtaa
      961 tggatgatcc ctgtccgttt aatcaggcac cgttttacaa cggaaaatcg gatacccgga
     1021 ctgttgacct gtctgatgct gtataccggc ggctgatact gatgaaagcc atgtcgaaca
     1081 ttactgactg ttccgttccg gatattaacc tgatgctgag atttatgttc ggaaaaaaac
     1141 gccgggctta tgttctgaat aatggcgggc tgaggatgag ttacgtcttt gagtccgcgc
     1201 tctcgttggc agaactggcg attatccagt cgtcgggtgc actgccatcc ccgccgggtg
     1261 tttatgtttc agtagtttta aaggagtccc gtaatgaagg ccagtgataa accccgccag
     1321 ctggcggtcc cctttgcgag taccggagat aaaaaccgta tcccggacaa ggcgacacag
     1381 cagaccagag agagcggtaa tgctgcgtat gattcaggtt ttcctccggt gaccatgaca
     1441 gcggtctcag cgggaggtat accgccacac ggcaaggatt ttaacggtct gatgtacgat
     1501 attaccgcag caatacggtt cgcccaggct ggcggtttgt acacgtataa tgccggtttt
     1561 gcgggggcca ttggtggata tgcaaaagga gccattctcg ccggagtcgc aacaacagcg
     1621 gtctggctga ataccacgga cgataacctg accgatcctg aaggctccga cagtgcgggc
     1681 tgggtaaatc ttcttgagga tccgaaaagg atattcctgc ggcagaagaa caatctgtca
     1741 gaccttcaga ataaagggac ggcacgggat aatcttcagg tttacagtaa agagcagtca
     1801 gatcaacgct atgttcatcg ggaaggcgat aaaataaccg gagagctgaa aatccgtggt
     1861 gttaatgcgc tgaggatttt caacgaagct tttggcctga tttttcgtcg ttcggaagag
     1921 tgcctgcacc ttattcccac cagtgaaggt cagggggaaa atggcgatat tggtcccctg
     1981 cgcccgttca ccattaattt gcggacgggt gaaatatcca tgtcgcataa agtgtctgtt
     2041 ggcggtggtt cgcaggtcaa tggtgcgctg ggtatcggcg ttcagaacgc cctggggggg
     2101 aattcaattg ttcttggtga taatgacacc ggatttaaac agaatggaga cggtattctg
     2161 gatgtttatg ctaatagtca
//
LOCUS       DAJTNW010000226         2125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNW010000226 DAJTNW010000000
VERSION     DAJTNW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(52..420)
                     /locus_tag="OO179_004169"
                     /pseudo
     CDS             complement(52..420)
                     /locus_tag="OO179_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
     gene            complement(459..746)
                     /locus_tag="OO179_004170"
     CDS             complement(459..746)
                     /locus_tag="OO179_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534005.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(996..1151)
                     /gene="hokD"
                     /locus_tag="OO179_004171"
     CDS             complement(996..1151)
                     /gene="hokD"
                     /locus_tag="OO179_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5534006.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1379..2044)
                     /locus_tag="OO179_004172"
     CDS             complement(1379..2044)
                     /locus_tag="OO179_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5534007.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          552 a          545 c          476 g          552 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aagcctcaaa
       61 cgactttagc aatgccgtat cacgggttct gcgtgtcgca actgtattca gatattgctc
      121 tgccgcttcg ctcagagctg gcgtatgttc ccggcctact gattcacaca ggtaatcaac
      181 gaaaccggac accagttttc gttcgcgagg cgtgatcgcc ccaccgaccg gagtccagta
      241 aacgaaaccc agttgcagga gtttgaaaaa acgcttgtgg aatgcgtagt tacgcacacg
      301 cttaaagtct gcgtgtatcc actcacctat tttgatttga tgcagaaaat cgcaactctc
      361 cggcgtcgcc gggagaagta atccggaaga ggtttgtttg accagttgta tatgtgccat
      421 cggctttctc cggtggcacg gtgttacaca gcaggagttc aatcctgctc aagattgtag
      481 atgagtttat tcttcagcaa aagcagaaaa ccagccttaa aaccaatctt tttcaaaacc
      541 cgtaatgatg tgacaaattc gtcctcacgc aaaataaaac cgtccgtcag aagtccatta
      601 caaaaataaa ataacacagc accgctcttc ctttgttgag attgcaaaca tctaatgcgg
      661 caatggctga caatcgctcc attctcaacg cgcacagcat agaggccatt ttcactaaaa
      721 atttcacgca attcttcgat tttcatcttc agaatccttc cagataaata gctctcccct
      781 gttcggggtc catccctctt ctccctgcgc gctacttaag tgagtcgatt ctatctgcga
      841 aggtgcgcga atcaaattca ccggaaataa acaacaaaaa acccgccgaa acgctgagag
      901 atcacagcga tgggcgggtt aagtgcgggt gcgttgagga tgcctgacac atcagaggtg
      961 ggcggggatg ggatcagctc cccacccggt cactcttact tcctggattc gtagtctacg
     1021 aagacagcga cctccgtctg gccggttcgg attcgtacct cacagaggtc tttcctcgtt
     1081 accagtgccg tcactatgac ggttaaacag atgacgatca gggcgattaa catcgccttt
     1141 tgctgcttca tagcctgctt ctccttgcct ttcggcatgt aagaggctaa cctacatgtg
     1201 caaagcatga aattggcctc agattaatgt taagcgtctt gccggacgcg taatgttaac
     1261 tggggctttt ctctatctgc cttttggtgt tcatgcctga agcagatagc ctcaagcacc
     1321 cgcagtcatt ctacttacct cgccaatatg aaatcaatca gaaaggtgcc ccataaaatc
     1381 actccttctc ttctttaccg tagtggagtt gaccaatttt gataagaggg cgtccctgag
     1441 atttgcggtg tagattggta tcgcgcagag aatacacaca gccacaatat tcctgctgat
     1501 agaatttttc gcgcttgctg atttcaatca tacgggacga gccgccctgc ttgcgccagt
     1561 tataatccca gtacaccatg cccggataat gcgcgacggc tcgccgccca cagtcgttaa
     1621 cctgctgcat atttttccag cgtgaaatgc ccagtgaact gctgatcaca ctgaaaccat
     1681 tttcagcggc gtacagcgct gtccgctcaa aacgcatgtc aaaacacatg gtgcaacgga
     1741 tccctcgttc gggctcccat tccattcctt tggctcgttc aaaccagttg tctgtgtcgt
     1801 aatcagcatc aataaacggc acgccgtgtt gttcagcaaa gcgaatattc tcatccttac
     1861 gaattaaata ctctttctga ggatgaatgt tcgggttgta gaaaaagatg gtgtagtcga
     1921 ttcccgaggc ctgaagcgcc tccatcacct caccggaaca tggagcacag caagagtgca
     1981 gtagtagttt gtttgccccg tttgggagct ccaatttggg ccgtttgaaa tcagcaactg
     2041 tcataaatat gtttattgag gtcataaaaa tatcaaagag tgtagcatta gaacggggct
     2101 atcggaaaca gatgtgtgac tgctc
//
LOCUS       DAJTNW010000227         2096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTNW010000227 DAJTNW010000000
VERSION     DAJTNW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO179_004173"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO179_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5534008.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO179_004174"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO179_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5534009.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAJTNW010000228         2055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNW010000228 DAJTNW010000000
VERSION     DAJTNW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.239"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            54..2055
                     /locus_tag="OO179_004175"
                     /pseudo
     CDS             54..2055
                     /locus_tag="OO179_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          546 a          461 c          513 g          535 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccactca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     1501 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     1561 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     1621 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     1681 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     1741 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat
     1801 tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg
     1861 ctggaaagaa ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag
     1921 gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat
     1981 aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac
     2041 agcgaagggg taacg
//
LOCUS       DAJTNW010000229         2047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTNW010000229 DAJTNW010000000
VERSION     DAJTNW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.240"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            6..224
                     /locus_tag="OO179_004176"
     CDS             6..224
                     /locus_tag="OO179_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5534010.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO179_004177"
     CDS             226..591
                     /locus_tag="OO179_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5534011.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="OO179_004178"
     CDS             588..1253
                     /locus_tag="OO179_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534012.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO179_004179"
     CDS             1253..1618
                     /locus_tag="OO179_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5534013.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          559 a          424 c          543 g          521 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgt
//
LOCUS       DAJTNW010000230         1975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNW010000230 DAJTNW010000000
VERSION     DAJTNW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            348..1712
                     /locus_tag="OO179_004180"
     CDS             348..1712
                     /locus_tag="OO179_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5534014.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSRPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            1762..1971
                     /locus_tag="OO179_004181"
                     /pseudo
     CDS             1762..1971
                     /locus_tag="OO179_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          517 a          369 c          450 g          639 t
ORIGIN      
        1 tactggcgtc ctgatgcgga caggactggt atggaacctg tttatgtgat acttaatgat
       61 cctctggatt ccgggcgatt tacgcgtaaa caactaaata aaaatatctt aaacatgcaa
      121 gtaattttgg tattctcgat acaagaataa atagtagtaa ctactatagt tgcaaataat
      181 gttaaaaaca tttctatgat catctttaca tttttcatga ttgacaattg tttttggtta
      241 aaaaaagaca actaatgagt gtgataatgc aatactttga gtgattatat aatgataagt
      301 aaacaggttt gcgacaattg gttatcagta ttacagaagg attaaatatg tcattattca
      361 cagaacctaa ggaaattgaa agattaccct ctgaagagat tgaacgatta tatcccgtcc
      421 tgcgatacag ggtctttatc agcattttcc tcggatatat ggggtattat tttgtccgaa
      481 acaccaccag tgtgttatcc ggtgtgctgc acatgtctgc aaccgagatc ggcattatct
      541 cctgtgccgg ttttttatcc tatggtatat caaagtttgt ttccggactt atttctgaca
      601 gaagtaattc aaaagtattt ttgtctcttg gtttattcct gtccggactg gtaaattttc
      661 tgataggata tattcccggg atcatcacat ccgttacgtt attttcaaca atgtatttac
      721 ttaacggctg gatccaaggt atgggatatc ctcctggtgc caaaacactg gtgttctggt
      781 atgaacatcg ggaaaggatc acctgggcca cactgtggaa tttatcgcac aatgtcgggg
      841 gagctctggc ccccgttctg attggtttca gttttggttt tttcggcgac agtgctctgg
      901 atcatgcccg ggccgcattt atcttcccag gagtactgtg tatggcaatg agtgtgctga
      961 tttactttat tcaggtggac agacctgtat cagtggggct tcctcccatt gaagaatgga
     1021 aaggtaacgt cgtttcacgc ccggctaaag gccgtgaaca aggtccgaga ctctccattc
     1081 ctgatatcat cagaaaacat atcatccgaa ataataaact catttactgc tgtatctatg
     1141 gttcgtttgt ttatattctg agatacggta ttgtttcctg ggctcctaag tttttaagtg
     1201 acagtctgga tgtcggtggt aaggatatgg gaaaactggc atcaatgggg ggggtttctg
     1261 tttttgaaat cggtggtgtg gctggtatgc ttcttgccgg atacctgtct gtcagactgt
     1321 tcagaaactc aaaaccatta acaaatactt tatttctggc acttaccatt attttattaa
     1381 tcgcgtactg gtacgtgcca tcaggcaatg aatatctctg gctaaactat acaatcctta
     1441 ttctgttggg gctggcagtg tatggtccgg tgatgtttat tggtctttat tcaatggagc
     1501 ttgttcctaa agaagctgcc ggagccgcat cagggctgtc aggcacattt tcctatatct
     1561 tcggctcaat tgttgcgaca ctgggaatgg gacttgttgt cgattatctg ggatgggggg
     1621 ccacatttat cgtactgata ctgtctgctg tttttgccat cattttcaca ctgatgagtc
     1681 gtgaacgctc actggagttc gaaaaagagt aattttctca ggaaaaacat aaaattactg
     1741 actaaatatt tattttttga atcgcagggc attccggata aggttgatat cgtgtcacgg
     1801 ctgaagacct atggatacag tatttctggt gtggagacgg atgacggata taaggcgctt
     1861 gtccgcgctt ttcagcttca tttccggcag aaaaattatg acggcataat ggatgctgaa
     1921 accgctgcca tcctgtatgc attgctggag aagtatttcc cgggtaaata aggag
//
LOCUS       DAJTNW010000231         1951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNW010000231 DAJTNW010000000
VERSION     DAJTNW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO179_004182"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO179_004182"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5534015.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1951
                     /locus_tag="OO179_004183"
     CDS             1647..>1951
                     /locus_tag="OO179_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5534016.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          516 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAJTNW010000232         1937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNW010000232 DAJTNW010000000
VERSION     DAJTNW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..546)
                     /locus_tag="OO179_004184"
     CDS             complement(<1..546)
                     /locus_tag="OO179_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534017.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1201..>1937)
                     /locus_tag="OO179_004185"
     CDS             complement(1201..>1937)
                     /locus_tag="OO179_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534018.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          545 a          441 c          417 g          534 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggaacctag gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa
      841 ggatgatgaa tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc
      901 ccgaagtcct aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg
      961 ggaagtcgct gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc
     1021 caaggtcata acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca
     1081 atagcgagta tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg
     1141 accatataca actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag
     1201 ttactctgcg gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac
     1261 gtcaaactca cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat
     1321 taactcaagg tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt
     1381 agcgtcttta gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc
     1441 tgtatcatcg cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg
     1501 acctgcgcgt tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc
     1561 gtaatcgctc agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag
     1621 atcttcagct tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc
     1681 tgacgtatct ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg
     1741 catatcgcat acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc
     1801 agacacatga gtagcagcag aacacaggat gcccgtctta ttggtctcag ggttcaacac
     1861 tggggtgaca gttacgcttg cccctttgat gactgtggta gacggagcat ccttagctgg
     1921 attaaacgcg ccattat
//
LOCUS       DAJTNW010000233         1916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNW010000233 DAJTNW010000000
VERSION     DAJTNW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(56..1705)
                     /locus_tag="OO179_004186"
     CDS             complement(56..1705)
                     /locus_tag="OO179_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5534019.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          508 c          492 g          465 t
ORIGIN      
        1 cctgaagagg ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg
       61 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
      121 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      181 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      241 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      301 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccaaaa
      361 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      421 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      481 cgcgtcgcca gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta
      541 ccgcatcgcg acgggcactg gccttctggc ggaacagcgc ttctgtctct ttctcgcctt
      601 tcagcgccga gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga
      661 tggggatgaa ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg
      721 aagagtaatc ccaaccggag gccgctcccg cgcgtaaatc gcggtacacc tcgttgggcg
      781 ggcgaccaga atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt
      841 cgcgatcgtc ccagtaacgg ttgagcagcg atccgtccgg catccgcaca acatggcgat
      901 aggcctgatt agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttaa
      961 ggtggtcgag atagcggcgc gcaccgcgta caccatcttc ttcaaacaac tccaccgtca
     1021 gcgcaaaaac cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt
     1081 gaccgtagtt ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc
     1141 gaccactttc cgccagtccc agcatggtga aataggaatc ccaatagtag gtttcgctaa
     1201 aacggccgcc cgggacaata tatgactgcg gtagcgccag cagagaagac cacggaatgt
     1261 gatcctgtgg ttcgcgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat
     1321 tttgcgggtc cgatacatac tcgctggagt agacctccgg cagccagaag tgattttcaa
     1381 caaacttgcg caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat
     1441 ccagcgggtc cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg
     1501 ccgactgaac atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg
     1561 cgggcagccc ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta
     1621 attcatacgg gtccagttca tagcagagat cgacctcgat catcagttcg tctggattag
     1681 ggttttgaat tttctgattg agcataagga tccaacctcc gaaattcgtc gctaattatc
     1741 gggcttttct ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga
     1801 tcacgtttat taacttgtca aaaaatataa cgcactaata tcaatggaat ttataaaaat
     1861 acggatttgg tgttgttttt cattttcaga acattcaatc ctgctctcgc cgcaat
//
LOCUS       DAJTNW010000234         1890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNW010000234 DAJTNW010000000
VERSION     DAJTNW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..951
                     /gene="virK"
                     /locus_tag="OO179_004187"
     CDS             1..951
                     /gene="virK"
                     /locus_tag="OO179_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5534020.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1016..>1890
                     /gene="lpxM"
                     /locus_tag="OO179_004188"
                     /gene_synonym="msbB"
     CDS             1016..>1890
                     /gene="lpxM"
                     /locus_tag="OO179_004188"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5534021.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHP"
BASE COUNT          547 a          363 c          421 g          559 t
ORIGIN      
        1 atgttttcta taagtaactt atcatttatc ggtttcctta aaaggattat tttttcctca
       61 gattcactac cggggaagtg ggaacacaga aaatttcggt tcatgtacat tttgcgatgt
      121 tctataaatc cggttgtcag tattcgatat tattatgaac tgcgttcctt gccgtgcatt
      181 gaggatattc tggcaataca ccccacgttg ccagcaagaa ttcatcgccc ttatttgcat
      241 aaaggggggc gcgcctggac ccgtggacaa tatattcttg agcattatcg ctttgtgcaa
      301 aatttgccag aaaaatattc taaatttttg tttccacaaa aatcggtatc attagtccaa
      361 ttcatcggga aagatggaga gaattttgat atccagtgtt ctcccagtgg ttttgaccgg
      421 gaaggagagt tgatgttgtc attgttttac aataaaacag ttattgcccg gttaacattt
      481 tcagtcattc tgactcaaaa tggacacatt gcttttattg gcggattaca gggagcccct
      541 aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg gattttccca
      601 aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgcaatat ctctgagtgt
      661 ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta tcagaaacgc
      721 aaaacctttg ttgcggtcta tagtgatttc tgggagtctg tagcaggaaa aacctgtggt
      781 gactggtaca gattaccaac ccaggtgatt cgaaagccgc taagcgatat agccagtaaa
      841 aaacgctctg agtaccgaaa acgatacgct ttgctggatt atatccatga aactactatc
      901 cgctctttgg atgcatatcc tgtacactca gaacatcaga atttaaatta aggtttgatg
      961 tattctctga ataaaacagt aatgataatt ttggtagagg cattcgcact aaataatgaa
     1021 aaaatataaa tctgagttta ttcctgaatt taagaaaaat tacctttccc ctgtttactg
     1081 gtctacatgg ttccttttgg gaatgattgc aggtatttca atgtttcccc cttcattcag
     1141 agatcctgtc ttggcaaaaa tagggcgttg ggcgggcaga ttgagcaaaa aagctcgtcg
     1201 cagggcgacg attaatttat cgctttgttt cccagaaaag agtgatacag aacgggaaat
     1261 aattgtcgac aaaatgtttg tcacagcatt gcaatccata gtgatgatgg cagaactggc
     1321 aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg aaattcttga
     1381 ggaaattcga cacaataata gaaatgtgat ttttctggtt ccccatggct ggagcgtgga
     1441 tattcctgca atgttgctgg cagctcaggg aaaaaaaatg gccgccatgt ttcatcagca
     1501 acgaaatcca gtgattgatt atgtctggaa ttcagtacgg cgtaaattcg gggggcgctt
     1561 acatgcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg
     1621 atattacctc ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc
     1681 gacctataaa gcgacattac caatcattgg acgtctgatg aacatcagtc aggctatgat
     1741 tataccactt ttcccggttt atgatgaaaa aaaacatcta ctgactattg aaattcggcc
     1801 accaatggat gcttgcattg ccagcgcgga caataaaacg attgcccgac aaatgaacaa
     1861 aacagtggaa attttggtgg ggccacatcc
//
LOCUS       DAJTNW010000235         1799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNW010000235 DAJTNW010000000
VERSION     DAJTNW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..142
                     /locus_tag="OO179_004189"
     CDS             <1..142
                     /locus_tag="OO179_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534022.1"
                     /translation="EFATKWGRAALPHERLTYFPLEDLIKNNGKKIRSDKKHFLECNE
                     DK"
     gene            complement(941..1277)
                     /locus_tag="OO179_004190"
                     /pseudo
     CDS             complement(941..1277)
                     /locus_tag="OO179_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          570 a          361 c          365 g          503 t
ORIGIN      
        1 ggagtttgcc actaaatggg gacgtgccgc gctgccacat gaacgcctaa cctattttcc
       61 acttgaagac ctcattaaaa ataatggcaa aaaaatcagg tctgataaga aacacttctt
      121 agaatgcaat gaagacaaat aacaacaatc tgcatattgc caccaaccac agatgtggca
      181 tcgttatcag cgcacttgat tttctttata ttcactgacg agcttgccgg ggcacctttg
      241 tttgccggtc attctggtat catgcagata tttacggcgt acaagcccac tagcatgaca
      301 ttgtaataag gcctgagcct aacgctttaa tgtgtgtcat acactcagtg gcgtaatcgt
      361 tgacaatatg gtgcatgtaa tcgtaagcgt ccagcgaaat ccatctggtc ataatctgaa
      421 gcatccgaca aagtggtgtc caccaaataa gtgatctgcg cccaatatcc tgagccggaa
      481 ctttgacaaa ggccaacgaa aaatgggagc ggatgttaca gagttctcgg tacaggggaa
      541 aagtggtacc tgtcgccagt tctccgatct gtttaaccgg gaaattatct cctacagctt
      601 gtcagaaagg tcggtgatgg agcatcgtta atacgatgct atacgatgca ttcacagtgc
      661 ccggccagag gatgccccgt cgctgcatat ggatcagggt tggcaatatc gaatcgcagg
      721 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
      781 ctgaataacg cagtgatgaa gaacttcttc agcacactgc taaaacgcat agtgatcgag
      841 cgctgattct ggcgaacaac tgaactaata catcagaata tgcattatgt taaataaata
      901 tgaaaagatg gtttaaatac cccgttactt ttgacttaca ctatacggta tcgcatcgtt
      961 taatattcgc accggccaga tttttatttc tattagttgt cacaatactg aatgcgtacg
     1021 accacagtat tctggctcct gtgtggttat gctttaattc tgcgttccgg gcagataagc
     1081 agttgcttgc aggaatcctt cttgtgttaa tgtcagttcc cctttttacc agtgctgatt
     1141 tccacattcc gtccaacaga gcttatagcc tttccctgga ttatagcatt gtccggctga
     1201 agttcttttt gaataataat agaagcactg ctggcagatc cagtccgttt ttcataaccc
     1261 actgtactga taaccataat ctaatcagta gaaattgagt cgaaaataag cactactcca
     1321 tacaggataa ttagaggtca gtttgattat tcacaattca tcatcagcat tttctatttc
     1381 tgacgaaatc aacatgaaaa taaccatata tgataattat tataataacg gctttaattg
     1441 gaatacatat attacaacgt attatatata attggtattc tgggaactat attctcaaaa
     1501 tatagtagaa acgaggtatg tttctggtgg aaaggacagt gggattaaaa agtaagaatt
     1561 gataaaaaaa acgccagcaa cacatagtgc cggcggaggg aataccccat ggagaaaatg
     1621 tgatgcctag agcatcatga atatataatt aaaaatagtt agcgttgtca atactttcac
     1681 aaaaataatt ttcgtagtat agaaagatat ttttatgcat gacctacctg aatttgctcc
     1741 gggtagaggt tataaataaa aattgaatca cgacaaacac aatattcata gtatggcga
//
LOCUS       DAJTNW010000236         1762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNW010000236 DAJTNW010000000
VERSION     DAJTNW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..444
                     /locus_tag="OO179_004191"
     CDS             <1..444
                     /locus_tag="OO179_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5534023.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OO179_004192"
     CDS             445..810
                     /locus_tag="OO179_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5534024.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            807..1490
                     /locus_tag="OO179_004193"
     CDS             807..1490
                     /locus_tag="OO179_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5534025.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1689..1761
                     /locus_tag="OO179_004194"
     tRNA            1689..1761
                     /locus_tag="OO179_004194"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1722..1724,aa:Ile,seq:cat)
BASE COUNT          495 a          378 c          480 g          409 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgtgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gagcactata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggagc
     1321 tggcattact ggcgggagtg acaactaaaa actggtccaa aacatttact cgtcactggg
     1381 ttgcaatgaa acacattttt caccgactgg atagtgaggc tttattgttt gtaatgagaa
     1441 cacgttcaaa aaaaagacgg cattttcaaa gcaaagtgtt gcaaaagtag attgaaaggc
     1501 atatattcat gcaaatctga tactttgccg attttgtacg tgatggcaaa agcaaacaaa
     1561 acccgcccac aagcgggttt tttgtgccac ttatctcgga tagacatggt gaatgcgctg
     1621 gtggaggaga taagggtgat ttttgaatgc ttgcaacatt gatttcgtaa cgttattatc
     1681 ctgcgcccgg ccctttagct cagtggtgag agcgagcgac tcataatcgc caggtcgctg
     1741 gttcaaatcc agcaagggcc ac
//
LOCUS       DAJTNW010000237         1687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNW010000237 DAJTNW010000000
VERSION     DAJTNW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..90
                     /locus_tag="OO179_004195"
     CDS             <1..90
                     /locus_tag="OO179_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5534026.1"
                     /translation="LKEKRKAGVLTKNTTPISDDEMVENGQHQ"
     gene            511..1089
                     /locus_tag="OO179_004196"
     CDS             511..1089
                     /locus_tag="OO179_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534027.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1198..1456)
                     /locus_tag="OO179_004197"
                     /pseudo
     CDS             complement(1198..1456)
                     /locus_tag="OO179_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1440..1687
                     /locus_tag="OO179_004198"
                     /pseudo
     CDS             1440..1687
                     /locus_tag="OO179_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          547 a          297 c          389 g          454 t
ORIGIN      
        1 ttaaaagaga aacgtaaagc tggagtgctg accaaaaaca caacgccaat cagtgatgat
       61 gaaatggtgg aaaacggaca gcatcagtaa cgaaagtatc ttagcaggca tgaaaatcgt
      121 ccgcatcccc taaacggtca aaatgcttct gccgtgaagg agggagaatg ccatctcatc
      181 acttatatct ggcgaatgaa acacagatgg tcggccttgc ccccaaacac acggcggtgc
      241 aaaatggaat ggcagaaaac ttcgtcaaaa cgatgaaata tgattaaatc agtgttatgt
      301 tcgaacctga cgtgttaaca gcagtaaagt agtctcgcga aggtgttcaa acattataat
      361 gaatggcatc ctcatagtgc gcagggttat cgcttgctgc ggaaatatct gaagcgacgt
      421 accagtaatg gggaaaatga tagctggttt ctgaaaatat agaggaaaat gtgatatata
      481 tatcctgtta atatttaagg agaatataaa atgaaagtct cgtttaagtc tttaggctat
      541 atttttcatg atatatataa taaaaaacac actatcgatg agttcaacga tgtggtaaga
      601 aaagcagttt tatcaggtaa aattaacgag ctgaatgcat gtcataaagt ggcaattttt
      661 cttgccgaaa aagacaatga gataacaaag aaagacaaag ccaagataat agatacatta
      721 actgaaaact attccattga atttcaacaa ttaatgaata ttagtgagcg cacgttaaat
      781 tcatctttat atattactcc tggtgaaagt ggttttgtca gttttgttaa tcgtgaagga
      841 aaaatatgtc atactgcata tgtaaaatca tcagataata gcatgacata ttaccatgca
      901 aatggtagca gcattgacaa atatattacg gatatgtgcg gccttatttg tatgaggcat
      961 attgagtcta ctggcattat tttttatatg cttgatgaaa aagttttatc tgccattgct
     1021 gaatttatga atgaaaaagg atggcgggct gcgttttgtt cagcaaagaa cttatataaa
     1081 tgtgtttaat tttagaaaaa ctatttggta aaatccaaca agagtcgaga ggttcgcgtt
     1141 gcttcagcag ggcctcagcc tgttcaacca gactcagttg caggtcgtga cgcagattta
     1201 gtggtggtgg ttgattgtca gataccagcc gatgatcttg tagtgcatat catcagactt
     1261 tgagaagcat atggttttgc gggtaagccg cttaagatgt gtccgcaggt tcagattatg
     1321 tcgctctatc cgctgagtgc atttcttgct tataacatga ctggttgagg acaataaagt
     1381 tcggtatacc ggctcaggca tcggtcatgt agaaggcaat gctaaactgg ctcagcaaac
     1441 taagagcagc gcgcatccac ttacccaatg cggttgagaa aatagccttc gatcgctttc
     1501 atgtggcgaa acaaccgggt gaggtggtgg ataaaacccg ccagaatgaa catccgcatc
     1561 ttcctgttga aagtcgtcgt caggcaaagg gaactcgctt cctgtggcag cacagcgata
     1621 aatggatgac cgaatccagg caggaaaagc tgatatggct gcgtgcgcag atgaagctga
     1681 cgagcct
//
LOCUS       DAJTNW010000238         1666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNW010000238 DAJTNW010000000
VERSION     DAJTNW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.249"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(110..577)
                     /locus_tag="OO179_004199"
     CDS             complement(110..577)
                     /locus_tag="OO179_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5534028.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO179_004200"
     CDS             complement(643..882)
                     /locus_tag="OO179_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5534029.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO179_004201"
     CDS             complement(1017..1622)
                     /locus_tag="OO179_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5534030.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          529 a          374 c          295 g          468 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attaca
//
LOCUS       DAJTNW010000239         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNW010000239 DAJTNW010000000
VERSION     DAJTNW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..636)
                     /locus_tag="OO179_004202"
     CDS             complement(<1..636)
                     /locus_tag="OO179_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5534031.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIQPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OO179_004203"
     CDS             complement(750..1322)
                     /locus_tag="OO179_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534032.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          317 g          469 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggctgaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTNW010000240         1596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTNW010000240 DAJTNW010000000
VERSION     DAJTNW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.251"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            174..1199
                     /locus_tag="OO179_004204"
     CDS             174..1199
                     /locus_tag="OO179_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5534033.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1267..>1596
                     /locus_tag="OO179_004205"
     CDS             1267..>1596
                     /locus_tag="OO179_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5534034.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFQNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIG"
BASE COUNT          379 a          370 c          411 g          436 t
ORIGIN      
        1 gcaccattgc ggggcgctgt ttttattatt tcctgtctac actcagctta ttgcgacgcg
       61 aaatcgtgcg caaaagctgg cactactttt gcttataaga ggatggccac agacaggtaa
      121 aagacgtttc ccaaacgtcc tataacgata attttcgcct cacaggaagc attatgaaaa
      181 agatgatgat agccacactg gctgccgcca gcgtgctgct tgccgttgca aatcaggcgc
      241 atgctggcgc gacgcttgat gccgttcaga aaaaaggttt tgtgcaatgc gggatcagtg
      301 atggattacc taggttctct tatgccgatg cagacggtaa gttttcaggt attgatgttg
      361 atgtttgtcg tggtgttgcc gctgctgtat ttggtgacga cacgaaagtg aaatataccc
      421 cactcacagc aaaagaacgc ttcaccgctt tacagtcagg ggaagtggat ttgctctccc
      481 gtaatacgac ctggacttca tctcgcgatg ccgggatggg aatggcattt accggcgtca
      541 cttattacga cggcattggc ttcctgacgc acgataaagc ggggctaaaa agcgcgaaag
      601 aactggatgg cgctaccgtc tgtattcagg cgggtactga taccgaactc aacgtcgccg
      661 actatttcaa ggcaaacaat atgaagtaca caccagtgac tttcgatcgc tctgacgaat
      721 cagcgaaggc actggaatct ggtcgctgcg atacgctagc ttcggatcag tcgcaactgt
      781 atgccttgcg catcaaatta agcaacccgg ctgaatggat tgtcttaccg gaggttatct
      841 ctaaagaacc gcttgggccg gtagttcgtc gtggcgatga tgaatggttc tcgattgtac
      901 gctggacgct tttcgccatg ctaaatgctg aagagatggg catcaattcc cagaacgtcg
      961 atgaaaaagc ggctaatcca gcaacgcctg atatggcaca tctgttgggt aaagaaggcg
     1021 attacggcaa ggatctgaag ttggataata aatgggccta taacatcatc aaacaggtgg
     1081 gtaactactc ggaaattttt gagcgtaacg taggttcaga aagcccgctg aaaattaaac
     1141 gtgggcaaaa taatctctgg aataacggcg gtattcagta cgcgccgccc gtgcgttaag
     1201 tcggtgtgag gtaacgagcg tcgctacggc ggcgctctct tcagcgttat gctttccgag
     1261 gtttctatgt ctcatcgccg ctcaaccgtt aaaggctcac tctcctttgc caaccctacg
     1321 gttcgtgcct ggttattcca gatccttgcc gttgttgctg ttgtcggcat tgttggttgg
     1381 ttatttcaaa acactgtaac gaatctcaat aatcgtggca ttacttcagg ttttgccttt
     1441 ctggatcgcg gcgctggctt cggtattgtc cagcatttga tcgattacca gcagggcgac
     1501 acctacggac gcgtttttat tgtcggctta ctcaatacgc tactggtttc tgcattgtgt
     1561 attgtgttcg cttctgtttt gggcttcttt atcggt
//
LOCUS       DAJTNW010000241         1565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNW010000241 DAJTNW010000000
VERSION     DAJTNW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..353)
                     /locus_tag="OO179_004206"
     CDS             complement(<1..353)
                     /locus_tag="OO179_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5534035.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMR"
     gene            complement(420..>1565)
                     /locus_tag="OO179_004207"
     CDS             complement(420..>1565)
                     /locus_tag="OO179_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5534036.1"
                     /translation="TESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDASRIEDIW
                     QFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTG
                     RTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLW
                     SSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDPT
                     PAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIGYIRTTITHAGGITGMRR
                     IADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPYSW
                     RFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLPVARLEDGTLLNW"
BASE COUNT          441 a          419 c          341 g          364 t
ORIGIN      
        1 cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg cattaatgac
       61 cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag caaaataccg
      121 gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac accaaactct
      181 gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa tgacgaacgg
      241 tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt ctgaatacga
      301 cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc catttttagc
      361 tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc gaaccgttat
      421 taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc cgggtcatac
      481 ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc atcgcctggg
      541 tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat ttgttcggaa
      601 tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg cagcgccgcc
      661 gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac ctgataaagc
      721 gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt gatcgtcgtg
      781 cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat actgttaaac
      841 acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa acattcctgg
      901 ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat gctcttccca
      961 aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag atgttcgtta
     1021 aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag atacttttct
     1081 gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata cgccagtcct
     1141 ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg cacacgaatc
     1201 gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc aatcgtacgc
     1261 ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag cagttgatac
     1321 aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat ggcagaaatg
     1381 gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa ttgccagata
     1441 tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc tttcagataa
     1501 gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca aagtccgctt
     1561 tcagt
//
LOCUS       DAJTNW010000242         1538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNW010000242 DAJTNW010000000
VERSION     DAJTNW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(910..1386)
                     /locus_tag="OO179_004208"
     CDS             complement(910..1386)
                     /locus_tag="OO179_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5534037.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAJTNW010000243         1510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNW010000243 DAJTNW010000000
VERSION     DAJTNW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..359
                     /locus_tag="OO179_004209"
     CDS             <1..359
                     /locus_tag="OO179_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5534038.1"
                     /translation="DYLCESVGREHTPALSDAAEQYLNTVATCRTRDTALLKSFDAFR
                     EWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVLWNWILFRKFS
                     SPEEVENVAAQLLEFA"
     gene            359..649
                     /locus_tag="OO179_004210"
     CDS             359..649
                     /locus_tag="OO179_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5534039.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            646..1200
                     /locus_tag="OO179_004211"
     CDS             646..1200
                     /locus_tag="OO179_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5534040.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            1341..>1510
                     /locus_tag="OO179_004212"
     CDS             1341..>1510
                     /locus_tag="OO179_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534041.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSP"
BASE COUNT          385 a          338 c          422 g          365 t
ORIGIN      
        1 ttgattacct gtgcgaatca gtaggtcggg aacacactcc agccctgagt gatgccgcag
       61 agcaatacct taatacagtt gcgacatgca gaacccggga tacggcattg ctaaagtcgt
      121 ttgacgcttt ccgcgagtgg gtaaccattc aggccggatt ttacaccgag catatttatc
      181 ctgatggtag tcgtgggcgc agggcaaaat ctatcgcatt tgcgaacatg gacgaaaccg
      241 agtttcagca ggtttataaa tctgtactga atgtgctgtg gaactggatc ctgttccgta
      301 aattttcctc tccggaggaa gtcgaaaatg tggccgcgca gttactggag tttgcgtaat
      361 ggtggattta cgtaaagcgg cgcgggggca gatgtgcacc gtcagaattc ctggctactg
      421 caatcacaat cccgaaactt ctgtgctggc gcattacagg ctggcgggga cgtgcggaac
      481 agcgacaaaa ccacacgata tgcaggcagc gattgcctgt agctcatgcc acgatttaat
      541 cgacgggcgg gtaaaaacca gcgattacac caaagaagaa ttacgcctga tgcatgcaga
      601 aggtgttttt cgcacacaag aaatctggag aaaggaaggt tatttatgat ttacccaaca
      661 aatacaggca aaagcgggga acaccttcgt ctcaccacgc tggaaagtgt ctggattcag
      721 ggaaaactgc gcatgtgggg gcgctggtcg tatattggcg gcggtaagac gggaaatatg
      781 tttaaccagt tgctggcatc caaaaaattg acgaaaacag ccgtcaatga agccctgcgc
      841 aggatgaaaa aagcgggaat agagaaacct gagctggaag cgtttttgcg agagatgatc
      901 aatggcaagc aaaagacctg gctggcgcat tgtactgatg cagaggcgtt atgtattgat
      961 agagtcataa gtgaggtgct ggcagagcat cctggattga ttagtgtcct ccggcaacgg
     1021 tatgaagggc gggggatgac taagcgtaaa atggctgaat tgctgaatga cgcacaccct
     1081 gagtggtgtt tccggacgtg ctgcagtcgg gtagatgtat ggctaaatct tgctgaatat
     1141 atgctctatc taccgatgcg tgatgcattc tcttccgggg atctaaaaac cgtttgttga
     1201 ctcaatctgt tatccggggc tatattcctc acgcgccagc aaaatctggc gtcgggattg
     1261 gcgtcccgga tgtttacgga gcgatatgag acgcgcccgc gtcttttttc atatcgtttg
     1321 cacagtcaca ttcgcgattt atggcgggct gtgtggggga gccgaaaggc tcgccggttt
     1381 ccgtacccgg ttacgccaac cctgcacagt tcgccaccag ttcgattggc gtcgtcggtg
     1441 gcgataattt ccaaatgtac ggagttatcg ttatgaccac tcaaatctct gttgaaactc
     1501 tctccccgat
//
LOCUS       DAJTNW010000244         1442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNW010000244 DAJTNW010000000
VERSION     DAJTNW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>1442)
                     /gene="sat"
                     /locus_tag="OO179_004213"
     CDS             complement(<1..>1442)
                     /gene="sat"
                     /locus_tag="OO179_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5534042.1"
                     /translation="VILNEEGTFNNIYLASGNGKVILNKDNSLGNDQYAGIFFTKRGG
                     TLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAINNEDSYIYHGNINGNIKLTHNI
                     NSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHATEHAIFRSTASHCSLVFLCGTD
                     WVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKTGVFKFDTLHLNNADFSISRNAN
                     VEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAH
                     NSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPE
                     QNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTI
                     SSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFT
                     GDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          376 a          280 c          277 g          509 t
ORIGIN      
        1 ctgcttgggc cagatttgca cgcagcacaa atgcactgtc atctatagta aggttgtcca
       61 ctgtaagggt tgtaaacttc ccattgtcac cagtaaaacg caccatactg tcttttgttt
      121 ctagacgatt aattgatgac tggctattta gatgccagat cgcattattc atgctaactg
      181 tagctttggg ggctgttatt gcgcctcgat aggcggtatc aaagccatac aaaagagtct
      241 ctgcccatgc ctgataagca gacgatatag tcggtgtttc agtttcaggc tgtcccagag
      301 taatggttgc tgcatcatca gcataaatat ttccggtgac agatgccata tttctggcaa
      361 tgaaatttgc atttccacca ctcagcctga atccatcagc agcgtagtgg agacccggcg
      421 ttacagtttt actattctgt tcagggattc ccatcatagt cagttcaccg gagatatcaa
      481 taccttttgt tgtgaacatg gaattttgtg ctataacttt tgctccttta tctatactta
      541 taggagtatt attcagaaag gttgcaccat taagtgtaac tacagcttga tcacctatgg
      601 ctatttggct gttatgtgca gtgatgccac ctgtaaattt agtttctcct atggatagat
      661 tagtactgat agtttgtttg aagtcaaaac cattattagt aatatttttc cctgcaagat
      721 tatcaatgta agcattttta tcgccgattg tgatagctga tttatttgct gatatatttc
      781 cttcaacatt ggcattgcgt gatattgaaa agtcagcatt gttcaggtgt aatgtatcaa
      841 atttaaacac cccggttttc cagtcaggct gatcaaatga ggtctgctga ttattacttt
      901 tgtaatcaga attgaacttt ttattatatg aactctctgt ttctttcaaa acggtgaccc
      961 agtccgttcc acaaagaaat accagggagc aatgactcgc tgtgcttctg aatattgcat
     1021 gctctgttgc atggccttgc atggtaagac tggcattact gacttcaaca tcattttttg
     1081 tgttgacact accatccaga attaattttg cattagtttt cttatcctga gaattaatat
     1141 tgtgcgttag ttttatattg ccatttatgt tcccatgata tatgtaggag tcttcgttat
     1201 tgattgccag aacggcttct ttcgttgtat ctgagttagt tattgttgtt ccatcgtcag
     1261 tggcggcaat tctagtaaaa gtctgattgt gtccatttaa atctagcgtg ccaccacgtt
     1321 tagtaaaaaa tatccccgca tattgatcat tgccaaggga attatcttta tttagtatta
     1381 ccttaccatt tccgcttgca aggtatatat tgttaaatgt tccttcttca ttaagaataa
     1441 ca
//
LOCUS       DAJTNW010000245         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTNW010000245 DAJTNW010000000
VERSION     DAJTNW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.256"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..667
                     /locus_tag="OO179_004214"
                     /pseudo
     CDS             1..667
                     /locus_tag="OO179_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            741..860
                     /locus_tag="OO179_004215"
                     /pseudo
     CDS             741..860
                     /locus_tag="OO179_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            993..1163
                     /locus_tag="OO179_004216"
                     /pseudo
     CDS             993..1163
                     /locus_tag="OO179_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1174..>1438)
                     /locus_tag="OO179_004217"
     CDS             complement(1174..>1438)
                     /locus_tag="OO179_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5534043.1"
                     /translation="LNTSFVVKKGKIEMLKIKGKSVDPNKKYRMTTFSFNATGGDGYP
                     RIDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
BASE COUNT          400 a          322 c          353 g          363 t
ORIGIN      
        1 ggcttctttg atgatatcga tcatgacctg ctactgaaga cagtgcagca tcatactcag
       61 gcacgctggg tggtgatgta cattgagcgc aggctgaagg caccggtgca aatgccggat
      121 ggagcaatgc tggcacgggg tcgaggaacg ccgcagggcg gcgtaatctc ccccctgctc
      181 tcaaatctgc tcctgcacta tgcctttgat atgtggatgc aacgtcagtt tcccggcgtt
      241 cccttcgaaa ggtacgccga tgatgttgta tgccactctc ggatatgact tcaaagtgcg
      301 tactctgaag aatttcaaag gcgaattgta cagaaaatgt atgccgggtg catcaaatgc
      361 agcaatgtgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct caacagctga
      421 gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga ttgagtacta
      481 tggaaagttc tggtccagaa acttcagcta tcgactgtgg agtgcaatgc agtcacgttt
      541 gcttaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc gaaagctggc
      601 gctggtaagg aaacagtatc cgaagctatt tgcccactgg tatttactac gtgcatcgaa
      661 tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga gcagccggag
      721 agtgaaactc tctccggcgg ggttccgata gcggtggcga acatgcggcg gtgttgtact
      781 cgctgatcgg cacatgccgt ctgaacaatg tggagccaga aaaatggctg cgttacgtca
      841 ttgagcatat ccaggactga ccggcaaacc gggtacgcga tctgttgccc tggaaagttg
      901 atctgagctc ttagtaaata tcaatatggt tctgacgagc cgcttacgca gatcatgcca
      961 taatgaataa actttaatca gcgaaattga cgaagaagct ggcagcgctc caacatcagt
     1021 taagccgcaa ggttaaattc agcaacaact ggcagaagca gaaacacaaa atacagcaac
     1081 tgcattccca tatcgcaaac atccgcagag actactttca caaagtcaca gcgactgtca
     1141 gcaaaaaaca cgtgatgatc gtcaattacg ttatcactgc caactgactt caccttttgg
     1201 ttcatatgaa gcagcatcca gcggtgaatg tttccggata tattcgataa gtacttcagc
     1261 atcaataaat ccggtattta tgtatccggg tctattatca atacgtggat agccatcccc
     1321 accagtagca ttaaagctaa atgttgtcat acgatatttt ttgttagggt caaccgattt
     1381 accttttatt tttagcattt cgatttttcc ttttttgact acaaagctgg tattcaaa
//
LOCUS       DAJTNW010000246         1419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNW010000246 DAJTNW010000000
VERSION     DAJTNW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(98..610)
                     /locus_tag="OO179_004218"
     CDS             complement(98..610)
                     /locus_tag="OO179_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5534044.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            805..>1419
                     /locus_tag="OO179_004219"
     CDS             805..>1419
                     /locus_tag="OO179_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534045.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFAD"
BASE COUNT          435 a          301 c          354 g          329 t
ORIGIN      
        1 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
       61 ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      121 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      181 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      241 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      301 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      361 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      421 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      481 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      541 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      601 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      661 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      721 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      781 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      841 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      901 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
      961 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1021 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1081 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1141 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1201 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1261 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1321 gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1381 gttggcacgc ttgcgacggt aggaaaaata tttgctgat
//
LOCUS       DAJTNW010000247         1415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNW010000247 DAJTNW010000000
VERSION     DAJTNW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.258"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..269
                     /locus_tag="OO179_004220"
     CDS             <1..269
                     /locus_tag="OO179_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5534046.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO179_004221"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO179_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5534047.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>1415
                     /locus_tag="OO179_004222"
     CDS             613..>1415
                     /locus_tag="OO179_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5534048.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHA"
BASE COUNT          382 a          365 c          381 g          287 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac ccgatcgcca gtaagcctga atccccggga
      961 cgaaaatctt cacgaaaacc gctgccggca gaacttcccc gggagacaca tcgccttctg
     1021 cctgctgaaa ccagttgccc ggcctgtgga ggtgttctga aagaaatggg ggaaacaatc
     1081 tcagagcaac tggatatcat taataccgcc tttaaagtta tcgaaaccat acgtcccaaa
     1141 ctggcctgta gccggtgtga tgtcatcgtt caggcaccac ttcctcctaa accgatcgaa
     1201 cgcggttatg ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat
     1261 atccctttat atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat
     1321 accatggtgc gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg
     1381 aatgactatg ttctggaggc aggaaaggtg cacgc
//
LOCUS       DAJTNW010000248         1380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNW010000248 DAJTNW010000000
VERSION     DAJTNW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.259"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..253
                     /locus_tag="OO179_004223"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO179_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1380
                     /locus_tag="OO179_004224"
     CDS             746..>1380
                     /locus_tag="OO179_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5534049.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          320 c          421 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
//
LOCUS       DAJTNW010000249         1357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNW010000249 DAJTNW010000000
VERSION     DAJTNW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.260"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(409..1149)
                     /gene="phoN2"
                     /locus_tag="OO179_004225"
     CDS             complement(409..1149)
                     /gene="phoN2"
                     /locus_tag="OO179_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5534050.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          390 a          265 c          285 g          417 t
ORIGIN      
        1 gattgatttt tttattaaaa aatggagcgg tatcaggcaa acgatttgag aggtgaccta
       61 aaatatattt aggttgtgcc ctgaatctga agttgtcatt tctgttatat tgatcgacat
      121 aggggatggt gcagaccgtt cccctgcaac accacattgc atgtggtgaa cggaaccgcc
      181 ccgagtttcc tagagaatgt tttatctgtg aactcaggtt gccagatcat cgtttccgat
      241 ggcagcataa taagcttttt ctgcttctgc cggagggata tgtcccagcc tttccagtaa
      301 tcccttaaat tcaggggtaa aacgtggtgt gcccatagac tcctcctatg ctcatatagg
      361 gtagatttgt ctatgggccc tccatagcct gatacaggct gtccagcttt atggggtcag
      421 ttcattggta ggagtattta attcttcaaa ctcttttttg gcttcgctaa ggcttttggt
      481 aaattcaggt gtattatgaa gtactgcaac aaccgatgct cccattaaac gcccagcctc
      541 tacatcgctt tgccaatgcg caccgcagat gacccgactt tctccaaact catatccacg
      601 tcgaagtatt tccgctttac gttgaggatt aatctccgca agtatcagtg ctactgccca
      661 accaaaggat gcatgaccag agggataaga gccagtgata gccattttct catctttatc
      721 aggtgtacag gttgcgtctt tataaataac gaatggacga actcttttat aatattcttt
      781 ggcgttacgc acggcgtaat catggctatc ctgtagtact tgtgttaaca actgatataa
      841 gattggggta ttttcccttg aaatatccat gccataagca tctgaaaatg caagaccaaa
      901 attctcgtag tatgcatctt cgctagcgag acgtacacga ttagcatccc ggagcgagcg
      961 gccgaattca taatgagctt tgtcagcctg aaatactact gaatcctctg ccggaggcgg
     1021 cggaagtatt gacaaactgt ctggtgaagt ttgttgagtg agaaaacctt ctgccttcag
     1081 agcatttgct gaggggataa aaatcatatt tgtagcaata caaaaaagaa gaaagttttt
     1141 ggttttcata atttatttcc ttagcataat taattgatga ttttggtcag ctgtaaaaaa
     1201 cgagctgtat tacaaaaaac acaagtcatg cggaaaaatt tgaacagagt gatggaaaaa
     1261 caaaaaatag tcttatatta aagtaataac attatttttc atattcacgg aaaataatgt
     1321 ggtaaaagaa tgatgttttt tatctatttt ttcgtat
//
LOCUS       DAJTNW010000250         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNW010000250 DAJTNW010000000
VERSION     DAJTNW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(41..100)
                     /locus_tag="OO179_004226"
     CDS             complement(41..100)
                     /locus_tag="OO179_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5534051.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..776)
                     /locus_tag="OO179_004227"
                     /pseudo
     CDS             complement(339..776)
                     /locus_tag="OO179_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382442.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1281..>1356)
                     /locus_tag="OO179_004228"
     CDS             complement(1281..>1356)
                     /locus_tag="OO179_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5534052.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          275 a          361 c          325 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctaaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAJTNW010000251         1336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNW010000251 DAJTNW010000000
VERSION     DAJTNW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(25..312)
                     /locus_tag="OO179_004229"
                     /pseudo
     CDS             complement(25..312)
                     /locus_tag="OO179_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            498..>1336
                     /locus_tag="OO179_004230"
     CDS             498..>1336
                     /locus_tag="OO179_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5534053.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPD"
BASE COUNT          378 a          246 c          286 g          426 t
ORIGIN      
        1 agtctgacat caagagcgcg gtagcctttt taatatttca ttttccattt caatacgttg
       61 tagctttttc ctgagctcac ggatttcaat ttgttccggg gtaatggggg aggcttttgg
      121 tgtttttccc tgccgctcat cacgtaattg tttcacccat cgcgtcattg tggaaaggcc
      181 gacatccata gcgctggctg catctgccac ggtgtatttc tggtcaacga ccagttgagc
      241 ggattcgcgt ttaaactctg cgctgaaatt tcttttttca ttatggcacc tgtgttgttc
      301 tgaggtgagc atatcacctc tgttcaggta gctaaattta gtgtgccacc tcaaggttct
      361 tagtgagccg tttttaaaag atatgataat atattattgt ttataaattg ttgatgtttg
      421 atttagtagt atataaagtt caccaccttt attaggcttt ccttttatct ctacttactg
      481 ggtgccagaa actttaaatt ttaaacggag ggggcatttt gtttaaagca aatcatgttc
      541 cggtattaat gtatcatcat gtcagccatt gcccaggact ggttacactt tctcccgaga
      601 cattccggaa acaaatgaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg
      661 aactggagtt tttttatcgg ggcggaaaac ttccccgtaa aagcgttatg ttgacatttg
      721 atgatggata ccttgacaac tggtttcagg tatatccttt attaaacgag tttaatctca
      781 aggcacatat ttttttaatt accagtttta ttggaaatgg tccggttcgt cactctcctg
      841 gaaaggaata ttctcatcgc gattgcgaac atcaaatagc aaccgggaat gctgataatg
      901 ttatgcttcg ctggtctgaa gtgaacgaaa tgttgcaatc tggtctggtc gaatttcatg
      961 ttcatacaca tactcataca cgttgggata aaaagttcac ttcacgagaa gaacagtgta
     1021 aacatcttcg gcaggactta ttgagcggga gagaatattt aaaaaaaatg acaggaaaat
     1081 gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt cagatcgctg
     1141 aagaacttgg attccattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag
     1201 gaactacacg gataggaaga ataagcacca aggaacggga aaattgcgta tggctgaaaa
     1261 gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccctt cataaggggc
     1321 caagattacc ggatga
//
LOCUS       DAJTNW010000252         1297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTNW010000252 DAJTNW010000000
VERSION     DAJTNW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            58..424
                     /locus_tag="OO179_004231"
                     /pseudo
     CDS             58..424
                     /locus_tag="OO179_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            352..825
                     /locus_tag="OO179_004232"
                     /pseudo
     CDS             352..825
                     /locus_tag="OO179_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          320 a          327 c          391 g          259 t
ORIGIN      
        1 aggtccacac taccgtcagc cggaaagccg ttgccgcagg cgaccgcgta aaccgtcatc
       61 aaggcaatgt gccgcgtact ccgggtggcc cgcagcggct ggtatacgtg gtgtcagcgg
      121 cggacaagga taaacacacg tcagcagttc cgtcagcact gcgacagcgt tgtccgcaag
      181 gcttttaccc agtccagcgt tacggtgccc cacgcctgac ggatgaactg tgtgctctgg
      241 ttaccacttt aacgtaaaaa ccgtggcagc cagtctgcgc cgtcaggaac tgagcgcaaa
      301 agcctcccag aagttcagcc cgatcagtta ccgtgcacac ggcctgcctg tatcagaaaa
      361 tctgctgacg caggattttt acgccagtgg cccgaaccag aagtgggcgg gagacatcac
      421 gtactattac agctcaccga ctgcggggaa acacggggcg ccgggttatt gaaggtgacc
      481 tgtccagcta cttcgacaca gtacatcacc ggctgctgat gaaagccgtc tgccgcagga
      541 tcagtgatac acgttttatg agactgctgt ggaaaacgcc gtgttgatgt tggtctcttc
      601 cgggcggtca gtgaaagtat gccacagggc ggtgtgatct cgccgttgct gtcgaacatc
      661 atactgaatg agttcgacca gtatctgaat aaacgctacc tgagtggaaa agccagaaag
      721 gatcgctggt actggaatca cagtatccag cgaggccgaa gtactgctgt caaagaaaac
      781 tggcaatgga aacctgccgt tgcttactgt tgctatgccg atgactgtgt gccacgaagg
      841 cgcgtcctgg ggacgtgagt ccctgtgcag ccatgcagca caagagatga gggaaggccc
      901 cttggtgtcg gcgtgcaggc gcaggcacca aaccatccac tgctgctgca tttaagagat
      961 gtggtggtga gcgagatggg aaaaggtcgg acgtgagccg atcaggtatg gttcgaagag
     1021 acgaacgaaa gtgaaccgct gatgatgtgt cgaaagcata tagatgatgt caaaacaatg
     1081 gtggagtcgt tcctgtggga tatgtttact gcctgcgcgg catccggcat aaaagcggcg
     1141 tgaagtcgat tcaggctctt gtggggaacg tggacctgtc accccgatgt gaagggagaa
     1201 gcgcaagtgg aagccccatg agcgccagag taccaatgcg gggtacaggg acggagcacc
     1261 aagcgattgc gcagtaaaca tgcttgacaa cttaatt
//
LOCUS       DAJTNW010000253         1289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNW010000253 DAJTNW010000000
VERSION     DAJTNW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            72..1265
                     /locus_tag="OO179_004233"
     CDS             72..1265
                     /locus_tag="OO179_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5534054.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          384 a          271 c          295 g          339 t
ORIGIN      
        1 ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa caattcaatg
       61 agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat gccttagcac
      121 catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta atcaacggag
      181 catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa gttaagaata
      241 agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat attccaatga
      301 ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta atagccgtag
      361 actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt ctgtgtgacg
      421 ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat aatccattga
      481 tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc agggttatta
      541 ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct ctgggctggg
      601 attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg cctgaaagat
      661 ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg gctggaaggc
      721 tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag aaatcggcaa
      781 aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca gacaaagaac
      841 agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac ttcagagcgc
      901 gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt cgtgatgaaa
      961 agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca ggaagaattc
     1021 tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc ggttatcacg
     1081 ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc cggcgggtta
     1141 tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt ttaagacgaa
     1201 cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg gtgctggttt
     1261 attagcagta attttgtggg gatccctca
//
LOCUS       DAJTNW010000254         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNW010000254 DAJTNW010000000
VERSION     DAJTNW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(334..480)
                     /locus_tag="OO179_004234"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="OO179_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="OO179_004235"
     CDS             534..716
                     /locus_tag="OO179_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5534055.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="OO179_004236"
     CDS             complement(817..1146)
                     /locus_tag="OO179_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5534056.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          347 a          292 c          303 g          335 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgc
//
LOCUS       DAJTNW010000255         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNW010000255 DAJTNW010000000
VERSION     DAJTNW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..246
                     /locus_tag="OO179_004237"
     CDS             <1..246
                     /locus_tag="OO179_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR5534057.1"
                     /translation="IESGISSAAAMEIISNVRENRQVTVPAELLETLTQIAEQALWKR
                     EWAARDHGFPLPEYVTRRQAMVDQARSLLKNNTHEND"
     gene            291..1061
                     /locus_tag="OO179_004238"
     CDS             291..1061
                     /locus_tag="OO179_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534058.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          255 a          347 c          386 g          272 t
ORIGIN      
        1 attgagtccg gtatttcttc cgccgcggcg atggagatta tcagcaacgt gcgcgaaaac
       61 cgccaggtga ccgtccctgc tgaactgctg gagaccctga ctcagatcgc tgaacaggca
      121 ctgtggaaac gtgaatgggc tgcccgggat cacggtttcc ccctcccgga atacgttacc
      181 cgccgacagg cgatggttga tcaggcccgc agcctgctga aaaacaacac acacgaaaac
      241 gactgatgtt atcgccgcct gtgggcggcg atacagggag ttcatgaatt atgaatgaaa
      301 cgctgaatgc tctgatttgt cgtcacgccc gtaacctgct gctggcgcag ggctggccgg
      361 aagagacgga tgttgaccaa tgtaacccga actatccggg ctggatcagc atttacgtgc
      421 ggctggatgc gccccggctg gcgacgttac tcgttaaccg ccacgatggc gtgctgccgc
      481 cgcacctggc ctccgccatt cagaaactga ccggaaccgg agcggaactt gttctgtccg
      541 gcagtcagtg gcagtcgctg ccggtacttc cggcggacgg aacgcaggtg tctttcccgt
      601 atgccggtga atggctgacc gaagacgaaa tcagggcagt tcttgatgcg gtgcgcgatg
      661 cagtatgcag tgtcagttgc cggggggcag aagatgcgcg gcgtatccgg gcggcgctga
      721 ccacaagcgg tcagacgttg ctgacccgcc agacgcgccg ctttcgcctg gtcgtgaagg
      781 aaagcgatca cccctgctgg ctcgatgaag acgatgaaaa cctgcccgtg gtgctcgatg
      841 ccatcctgaa ccggggcgca cgtttttcgg cggtggaaat gtatctggtc agcgattgta
      901 ttgagcatat cctgtccagt gggctggcct gcgatgtgct gcgtataccg gatgaaccgc
      961 cccgccggtg gtttgaccgt ggtgttctgc gggaagttgt ccgggaagcc cgggccgaaa
     1021 tccgcagcat ggcggatgcc ctggcaaaaa tccggaaatg atgacgatgg cggggaaaat
     1081 ctatggtcac ccccgttttt gcaacactga tttttgatta atggtgggct tgcttaaatc
     1141 tatccggcgt ctctgtgggc aagggatgcc cgcgcctcga tgagttccgc acctgataat
     1201 cctaaaaatc ccctcggctt cagtgagcca ttttcccctg ttaggttttt aatcaggtcg
//
LOCUS       DAJTNW010000256         1259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNW010000256 DAJTNW010000000
VERSION     DAJTNW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            34..435
                     /locus_tag="OO179_004239"
     CDS             34..435
                     /locus_tag="OO179_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5534059.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO179_004240"
     CDS             447..818
                     /locus_tag="OO179_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5534060.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="OO179_004241"
     CDS             805..>1259
                     /locus_tag="OO179_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5534061.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAJTNW010000257         1217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNW010000257 DAJTNW010000000
VERSION     DAJTNW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            201..>1217
                     /locus_tag="OO179_004242"
     CDS             201..>1217
                     /locus_tag="OO179_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5534062.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKR"
BASE COUNT          257 a          380 c          327 g          253 t
ORIGIN      
        1 gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg atccgggggg
       61 ggatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg tcaggcactt
      121 ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta tctggttaac
      181 ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc caggtcttta
      241 tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt tcaggtaaac
      301 gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta atatctgacc
      361 tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc ggtgcgttac
      421 ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga ctggccatgc
      481 gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac gctgccatca
      541 ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg gctgacgagc
      601 aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca cagacaacgc
      661 aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcaccg gagcgcgttc
      721 tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag
      781 aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc
      841 gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg
      901 ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc
      961 tgcgtaagca aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc
     1021 tttacccggt cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca
     1081 ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg
     1141 cgccggtaac tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta
     1201 ataaagctct caaacgg
//
LOCUS       DAJTNW010000258         1193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNW010000258 DAJTNW010000000
VERSION     DAJTNW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>1193
                     /locus_tag="OO179_004243"
     CDS             <1..>1193
                     /locus_tag="OO179_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5534063.1"
                     /translation="VYKITVLHTNDHHGHFWRNNHGEYGLSSQKTLVDNIRQKVINNG
                     GSVLLLSGGDINTGVPESDLQKAEPDIRGMNLIGYDAMAVGNHEFDNPLNILRQQEKW
                     ATFPFLSANIYQKSTGRRLFSPWKIFIRQNLKIAVIGLTTDDTAKTGNSEYFTDIEFR
                     QPAAEARSVIDELNQQEKPDIIIAATHMGHYDNGESGSNAPGDVEMARSLPTGSLAMI
                     VGGHSQAPVCMASDNKKQWNYIPGTTCVPDKQNGIWIVQAHEWGKYVGQADFEFCNGT
                     MKLVNYQLHPVNLKMRITREDGKTEFSFYTPEITEDPQMLSLLTPFQNKGKAQLDVKV
                     GVVNGRLEGDRSKVRFVQTSMGHLILSALTERIDADFAVVSGGEIRDSIESGNITYKD
                     ILKVQ"
BASE COUNT          376 a          216 c          277 g          324 t
ORIGIN      
        1 aagtatataa aataaccgtt ctgcatacaa atgaccacca tggacacttt tggcgtaaca
       61 atcatggtga gtacgggtta tcctcccaaa aaactctggt ggacaatatc cggcagaaag
      121 taataaataa tggcggaagt gttctattat tatctggtgg tgatattaac accggtgtac
      181 ctgaatcaga ccttcagaag gctgaacccg atattcgtgg aatgaatcta attggttatg
      241 atgcaatggc cgtcggtaat catgaatttg ataatcctct aaatatatta cgtcagcagg
      301 aaaaatgggc cacatttcct ttcctttctg ctaacatata tcaaaaaagt acagggaggc
      361 gtttattcag tccatggaaa attttcatcc gtcagaattt gaaaattgca gtcattggct
      421 tgacgacaga tgatactgcg aaaacaggaa attctgaata ttttactgat attgagttcc
      481 gtcaacctgc tgctgaggcg aggagtgtca ttgatgaact taatcagcag gaaaagccag
      541 atattattat tgcggcaaca catatgggtc actatgacaa tggcgagtca ggttcgaatg
      601 ctcctggtga tgttgaaatg gctcgcagtt tgccgactgg ctcattagca atgattgtgg
      661 gaggacattc acaagctcct gtctgcatgg ccagcgataa taaaaaacag tggaattata
      721 ttccggggac aacctgtgta ccagataaac aaaatggcat ctggattgta caagcgcatg
      781 agtggggaaa atatgtcggt caggctgatt ttgaattctg taacggtaca atgaaactgg
      841 ttaattatca gttacaccca gttaatctca aaatgagaat taccagagaa gatggaaaaa
      901 cggaattttc tttttacacc cccgaaatta ctgaagatcc acagatgtta tccttactga
      961 caccattcca gaataaaggc aaagcacaac tggatgtgaa ggttggggtt gtgaacggac
     1021 gtctggaagg ggacagaagt aaagttcgtt ttgttcagac cagtatgggc catctgattc
     1081 tgagcgctct gacagaacgt attgacgcag attttgctgt tgtcagtggt ggggaaattc
     1141 gagattccat agagagtggt aacatcacat acaaagatat actgaaagtt cag
//
LOCUS       DAJTNW010000259         1162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTNW010000259 DAJTNW010000000
VERSION     DAJTNW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            383..>1162
                     /gene="uacT"
                     /locus_tag="OO179_004244"
     CDS             383..>1162
                     /gene="uacT"
                     /locus_tag="OO179_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5534064.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPILDPVSILTM"
BASE COUNT          266 a          266 c          262 g          368 t
ORIGIN      
        1 aacgggcata tattaggtag catgaccgtt ttaattataa ttttgcgact gagttcaaat
       61 tattgcccct caatctatgc cgctttcatt atttcaacat tgccgatcaa aagagcgcta
      121 tccagataaa aacgattaaa tgcgagagtg cgacatgcca gaatgattaa tgaaatatca
      181 atatgagaaa attacagata attccattgt caggttctct tgtcattttc gtggtttaag
      241 cacgattatg tgattgcctc cgcaattcct gtctctaact ccccttcctc gcaaaaactg
      301 gcactccacg agcatgtgtt tagacagttt cattaacgta aacggttgct ttttattctg
      361 gcgggcgaaa ggagaaacac tgatgagcgc catagattcc caacttccct catcttctgg
      421 gcaagaccgc ccaactgatg aggttgaccg catattatca ccaggaaagc tgatcatact
      481 cggtctgcaa cacgtccttg tcatgtacgc aggtgcagtc gctgttcctc ttatgattgg
      541 tgaccgactg ggcctctcaa aagatgctat tgcgatgctc attagctcgg atctcttttg
      601 ctgcgggatc gtcacattat tgcaatgtat cggtatcggc cgctttatgg ggatccgcct
      661 gccggtgatt atgtcggtga cctttgctgc tgtaacacca atgatagcca ttgggatgaa
      721 cccggatatc ggcctgctgg ggatatttgg tgccactatc gccgcgggtt ttatcaccac
      781 attattagcg ccacttattg gtcgcttgat gcctttattc ccgccactgg ttaccggtgt
      841 ggttattact tctattgggc ttagcatcat tcaggtgggt attgactggg ccgctggagg
      901 taaagggaat ccgcaatatg gtaatcccgt ttatttaggt atctcctttg ccgtcttaat
      961 ttttatcttg ctcattactc gctatgcgaa aggattaatg tccaacgtcg ccgtattact
     1021 ggggattgta tttggctttt tactttcgtg gatgatgaat gaagtcaatt tatccgggct
     1081 acatgatgcc tcgtggtttg cgattgtcac cccgatgtcg tttggtatgc cgattctcga
     1141 tcccgtttcc attctgacca tg
//
LOCUS       DAJTNW010000260         1112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNW010000260 DAJTNW010000000
VERSION     DAJTNW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..720)
                     /gene="virK"
                     /locus_tag="OO179_004245"
     CDS             complement(<1..720)
                     /gene="virK"
                     /locus_tag="OO179_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5534065.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKR"
     gene            complement(725..>1112)
                     /gene="capU"
                     /locus_tag="OO179_004246"
     CDS             complement(725..>1112)
                     /gene="capU"
                     /locus_tag="OO179_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5534066.1"
                     /translation="YQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMVL
                     AEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMAR
                     LAKQDIEERFDINKTALKILTLAKHK"
BASE COUNT          344 a          261 c          217 g          290 t
ORIGIN      
        1 gcgtttctga taccagtatc tcaattgccg gaaaacatga ctgtgctcac tgactgccag
       61 acactcagag acattacaag ctttcatcaa cgcacaaaat gcttcaaaaa taatccgttt
      121 tgggaaaagc ccataacagg cccgggttgc gcaccgaata acatcaggtc cggtattttt
      181 gggggctccc tgtaatccgc caataaaagc ggtgtgtcca ttttgagtca gaatgactga
      241 aaatgttaac cgggcaatca ctattttatt gaaaaacaat gacaacatca actctccttc
      301 ccggtcaaaa ccactgggag aacactggat atcaaaatcc tctccatctt tcccgatgaa
      361 ttggactaat gataccgatt tttgtggaaa caaaaattca gaatattttt ctggcaaatt
      421 ttgcacaaag cgataatgct caagaatata ttgcccccgg gaccaggctc gccccccttt
      481 atgcaaataa ggtcgatgaa ttcttgctgg caacgtgggt tgtattgcca gaatatcctc
      541 aatgcactgc aaggaacgca gttcgtaata atatcgaata ctggcaaccg gatttatagc
      601 acatcgcaaa atgtacatga accggaattt tctgtgttcc cacttccccg ggagtgaatc
      661 tgaggaaaaa acaatccttt taaggaaacc gataaatgat aagttactta cagaaaacat
      721 attgttactt gtgcttcgct aatgtgagta tttttaacgc agttttatta atatcaaacc
      781 gctcttctat atcctgtttt gccaggcgag ccatctgata aaaacgccca gggtcattaa
      841 aaaaatcatt cagggcgcac atccatgcgt gcttattact tgctggtaac aatgtcccgg
      901 tctggttgtt ctgaataacc tcagggattc caccaatctg agtggccact acaggcacag
      961 aaaatgccga tgcttctgcc agcaccatac caaaagattc gttttctgaa ggcagaacca
     1021 ccagactggc aacccgatat acgggggcgg caggaaaaac attgtcagca ataaaaacat
     1081 catcatgcat gcccatactg tcaatctgat ac
//
LOCUS       DAJTNW010000261         1103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNW010000261 DAJTNW010000000
VERSION     DAJTNW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(130..255)
                     /locus_tag="OO179_004247"
     CDS             complement(130..255)
                     /locus_tag="OO179_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534067.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHS"
BASE COUNT          256 a          247 c          369 g          231 t
ORIGIN      
        1 cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat agctgatgga
       61 gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg cgtttccggc
      121 gtaggtgtgt cacgaatgca tccagtggcg tgatgttgga tgcaatgctg tatgagagat
      181 gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt taagagcggt
      241 ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt tgcggagatg
      301 aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt cgaaaatgtg
      361 ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg cggcatccgg
      421 catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac ctgtcgtctc
      481 gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc gatgcgagac
      541 acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg gagcgaaggg
      601 ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc ccatgagtca
      661 gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc gggtcaaagc
      721 taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg ttaccaatcg
      781 aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat tgcccgcaaa
      841 cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg aattcgtcac
      901 gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat ggttcaacga
      961 caagagccgt atgacgcgag agtgtcacgt acggctccgt gggagcgtag gggggcggtg
     1021 cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt aaatgccgct
     1081 gacgcggcat ctgtaatctg tta
//
LOCUS       DAJTNW010000262         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTNW010000262 DAJTNW010000000
VERSION     DAJTNW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..1091
                     /locus_tag="OO179_004248"
                     /pseudo
     CDS             1..1091
                     /locus_tag="OO179_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          333 a          194 c          258 g          306 t
ORIGIN      
        1 agctagagag tctctaggca ttcaaggttc tgaagaactt tttagaaata tgattggcta
       61 ttctcgtctg atggggcgaa gtgaagaaga gattaagcga gcactgacag cattatcaca
      121 gatggctggt aagggtcaga ttatggctga agagcttaag gggcaactag ctgaagctgt
      181 tcctggtatg gttcaggtat tcgctaaagc aaccggaaag actgaacagg aattatttga
      241 tgctatgaag aagggggctt taaaatctgc cgatactctt cagaaggtca ctcaggaact
      301 gaataaacaa atcaccgcta aaggtggctg gaaagctatt tcagaaagta cacaggcaca
      361 actagggaac ttgaaaaact cttggaatac cacgcttgat agtatcttca gaggttctga
      421 aaatggtctg caagatttta caagaagctt aaccaatctt ttaaacgctc ttggtggaag
      481 tggtaagagt cttggtgaat ctcttggtag tctcatgact tctatgtcac atggtgtcga
      541 ttcattgacc gatattagtt acagggtaag ggcgttcttt gatgaggtga cattagctta
      601 ccgtccacta aatgacactc agaggaaaat tgttgatggt ttatctggtg ggttaattga
      661 tggtctgaag atgcttgctg tggctgttac tgctcaaaag gctgcaaatg tagcaggagg
      721 cttgttaaac cttgcaaacg ctgttacttc attcggtgac gctgttaata aagctaaagc
      781 tggcaaggct ggttcttcag gtggtgcgct aaataaagtc ggtagagttg tcctttatgc
      841 tgagttggta aattttgctc ttgataattt aaaccctgaa gcagctaaga atccgaactt
      901 acatcaatca ggtattggca cagaaacaaa cttcaaccct gaatacaaag gtgattcatc
      961 taagccaata gttctatcgc tgactggttt aatggtgcaa ctaataaaat tgctaacttc
     1021 caagaaccga tgaataactt cgtaaataac acacaatcaa ctccatcagc tatcacaggt
     1081 gacatggtta a
//
LOCUS       DAJTNW010000263         1090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNW010000263 DAJTNW010000000
VERSION     DAJTNW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..1018)
                     /gene="ltrA"
                     /locus_tag="OO179_004249"
     CDS             complement(<1..1018)
                     /gene="ltrA"
                     /locus_tag="OO179_004249"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5534068.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFSFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNF"
BASE COUNT          250 a          295 c          231 g          314 t
ORIGIN      
        1 tgaaatttct ggaccagaac tttccgtagt actcgatcca gcctctcact atcgcattgt
       61 agcgcctcgc aaaatccagc aaactctcag ctgttgagcg atgtatacgc cacttcttga
      121 ttgtttctgt tattttgcgc attgctgcat ttgacgcacc cggcatgcat tttcggtacc
      181 gttcgccttt gaaattcttc agcgtacgca ctttgaagtc atatccgaga aaggagaaac
      241 tcgttgcgac gtttctcctt ttaaacgtgt caatgtaggc gatattcgtt ttccctgcat
      301 tcaggaccag cccgacttcc gagaagcgct ccgataacct gttctttagc cttgttgcat
      361 cactcattcg tgaacaatgt actacaatat cgtcagcgta cctctcaaac ggtacccccc
      421 gatattctct ttccatccac aaatcaaatg catagtgaag gaacaagtta gcaagtagcg
      481 gactaatgac cccgccctgc ggagtgcctc gtgttcgggt tattaattct ccattttcac
      541 aactctgcat tggcgcttcc atccagcgtc gacagtacag gatgacccat ttaggcatcc
      601 cgtggtgttc cagagctttg agcaccagat catgtctcac atggtcgaaa aacgcactga
      661 tgtccacttc cagtatccag ctataacgcc agcaccggat ggcacattgt ttcagagcgt
      721 catgagctga tttgcctggt cggtaaccat atgaatcagc atggaaaatc gggtcaagct
      781 tttcttccat gaaaagtttt atcgctccct gagcaattcg atcggatact gttgggatcc
      841 ccagaatgcg ttccttgcca ttcgacttcg ggatccgttt ttccagcact ggtggcggaa
      901 accatgttcc cgaacatagc cggttccaga tcttgtatag attgccatca cgttgctggt
      961 cgaacatttt caaagtctgc ccgtcgcatc cggggggctc ccttgttgcg acgcacatcc
     1021 agatatgatg cccagaccag tgcttttggt atttcaaatg attttcgttg catcagttac
     1081 gtcatcccct
//
LOCUS       DAJTNW010000264         1032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTNW010000264 DAJTNW010000000
VERSION     DAJTNW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.275"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            597..>1032
                     /locus_tag="OO179_004250"
     CDS             597..>1032
                     /locus_tag="OO179_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5534069.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADY"
BASE COUNT          286 a          192 c          243 g          311 t
ORIGIN      
        1 taactgtgaa tagcttgctt gcaccgtatt gttcttgttt ttcggttagt taaacatcgt
       61 acttcatatt tgaacgttct gccggaatgc attatcaata gaggtaaagt cgcaacccca
      121 aatcgtaaag gaaaccgtag cacgtcgtat gcaagaacgt gccacggctg gctgatggat
      181 gttcgatagc gcgagtttga atgaaaatca gccggagatg attttacata attgctacgg
      241 aattattcaa tacaggaatt gcttgcgtct gcatggattg acctgaaata ttcccgaaaa
      301 tttctctaaa aaactcgaaa aaaatggtaa ctggttgaat gtattaatat gcaatggtac
      361 gtgtcaggga ttaaaagatg aacgtaaatt tattcaacgc attaatttta aagggtttta
      421 ttgtttgttg acgaaaacag gaatcatgtt cggtctcttt ttatctgtta aaagccagaa
      481 gcatttcctt cgctgacttt atagtcaacc ataacacaca ctctactgtc tgagtccagc
      541 gttttttaac attcttgtta agattatgtg atctttagcg cgggaggaaa atattgatga
      601 aacagcctgc gcccgtttat cagagaattg cgggtcatca atggcgacat atctggcttt
      661 ctggcgatat acacggttgt cttgagcagt tgcgccgcaa attatggcat tgtcgttttg
      721 atccgtggcg agatttactt atctcagtgg gagacgttat cgatcgtggg ccgcaaagtt
      781 tacgttgtct gcagttactg gaacaacatt gggttcgtgc ggtaagaggc aatcatgaac
      841 agatggcgat ggatgcgctg gcattccagc agatgtcttt gtggttgatg aatggcggcg
      901 actggtttat tgcgctggca gataatcaac agaaacaagc gaaaacggcg ctggaaaaat
      961 gtcagcattt gccctttatt cttgaagtac acagtcgcac cggcaagcat gttattgctc
     1021 atgccgatta tc
//
LOCUS       DAJTNW010000265         1021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNW010000265 DAJTNW010000000
VERSION     DAJTNW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>1021
                     /locus_tag="OO179_004251"
     CDS             <1..>1021
                     /locus_tag="OO179_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5534070.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSG"
BASE COUNT          316 a          192 c          264 g          249 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatcag gtttcattat
      421 caggtcagaa tagtggtggt gtatggtggg atgtagcgac aaatataact aacgctcatc
      481 agtcacaacc gaagagcacg atgaacattg tgcaagttgg caaaaatgga tcatatggtc
      541 agtttagtag ccattacagc tccagcgaaa acatgaaaca gctaggtgca aatttaagcg
      601 gtgggatact tattacaagg gatggactta catttggtca aaatgtggat gggacattag
      661 cccttataga ggcacccgga gcaacggggg ttaacgttaa tggctggccg ggactgagta
      721 ctgattttcg tgggtatgca atactgccag tgcagccata ccgaagagac gatgttattc
      781 tggatgaaaa aacgataggt aagaattacg acttaccaca aactagtcaa ttggttgtac
      841 cgacagctgg agccgttgtg cctgcgacat tggcagtgaa gagcggggat aaaggattag
      901 taaccttaaa acagaaagag ggtaaacccg tccccttcgg cgctgtgatt agttatagca
      961 aagacactga aaatatggcc ggcattgtag gtgaagatgg tattgcttat gtttctggct
     1021 t
//
LOCUS       DAJTNW010000266         1015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNW010000266 DAJTNW010000000
VERSION     DAJTNW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..375
                     /locus_tag="OO179_004252"
     CDS             <1..375
                     /locus_tag="OO179_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534071.1"
                     /translation="AILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFIAVV
                     RLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVA
                     TPFVLVGLGLIFPLLARKANSK"
     gene            complement(376..996)
                     /locus_tag="OO179_004253"
     CDS             complement(376..996)
                     /locus_tag="OO179_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5534072.1"
                     /translation="MNAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGF
                     DAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQCVVFRYPS
                     GKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECV
                     LEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          258 a          232 c          243 g          282 t
ORIGIN      
        1 gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct ctataaatgg
       61 ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt tgttgcattt
      121 attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat tcgtaataag
      181 cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc ctgtttgacg
      241 gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt ggcgctgaat
      301 gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct ggcgcgtaaa
      361 gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct gacccgtttt
      421 tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg ggtaatagac
      481 ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac gaccagtatc
      541 aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca tcaaaacggc
      601 ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa tttccagctc
      661 ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa cacattgatg
      721 attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact ctggcattgc
      781 tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt tttcgacgat
      841 gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa gagaatcgtc
      901 agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca gtaaaggcat
      961 taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg tcggt
//
LOCUS       DAJTNW010000267         1000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNW010000267 DAJTNW010000000
VERSION     DAJTNW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..1000
                     /locus_tag="OO179_004254"
                     /pseudo
     CDS             1..1000
                     /locus_tag="OO179_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          266 a          248 c          289 g          197 t
ORIGIN      
        1 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
       61 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
      121 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
      181 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      241 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
      301 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
      361 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
      421 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
      481 ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact caccggtata
      541 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
      601 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
      661 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
      721 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      781 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      841 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      901 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      961 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJTNW010000268          991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNW010000268 DAJTNW010000000
VERSION     DAJTNW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            45..>991
                     /locus_tag="OO179_004255"
     CDS             45..>991
                     /locus_tag="OO179_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5534073.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSV"
BASE COUNT          273 a          253 c          236 g          229 t
ORIGIN      
        1 tcatcactcg cagtaatttt aatctgagtt gaggttaaaa aacaatgaac accgataaac
       61 aagtttaccc actgtattac gaagcaaaaa atgacaaagt aagaaaacgt ctcggtatta
      121 aaggcggttt ttactgggct gaagcgaaaa aattatccat tgccatctcc cgtggtgctg
      181 ttgcgattga cgatgcaggc tacgatgaag atgactttaa aaaacctgtt cgcgtcaatt
      241 tgcccgttgt tgatgacctc ccgccagaag gcgtatttga tacggaattc tgcaaccgtt
      301 acgaaaaagg cggggaagat ggcatcacaa tggtatttat cgcatcttct ccctctgttc
      361 aggacaaacc ggccagcact gacaatacca acgtcaacgg cgaagacatg acagaaattg
      421 aggagaacat gctcctgcca gtttccggtc aggaactgcc cattcgctgg cttgcgcaac
      481 acggcagcga aaaaccagta acgcacgttt cacgcgacga actccaggca ttacacattg
      541 ctcgggatga agaactacca gctgttactg ccctggctgt ttcccacaaa accagcctgc
      601 tcgatccgct ggaaattcgc gatctccaca aactggtgcg tgatactgac agagttttcc
      661 ctaatccagg caattcaagc ctggggctga tgactgcttt tttcgaagca tacatggacg
      721 cagactacac cgatcgcggt ctgctgacaa aagagtggat gaaaggaaat cgtgtttcac
      781 gcatcacgcg cacggcttcc ggcgctaatg ctggcggcgg gaacctcacc gatcgcggcg
      841 aaggtttcgt tcacgatctg acgtcactgg cgcgcgacgt agccactggc gtactggctc
      901 gttcaatgga cgtggacatt tataaccttc atccggcaca cgctaaacgt gtcgaggaaa
      961 ttatcgctga aaataaaccg cccttttctg t
//
LOCUS       DAJTNW010000269          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNW010000269 DAJTNW010000000
VERSION     DAJTNW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO179_004256"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO179_004256"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5534074.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTNW010000270          979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNW010000270 DAJTNW010000000
VERSION     DAJTNW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..979
                     /locus_tag="OO179_004257"
                     /pseudo
     CDS             1..979
                     /locus_tag="OO179_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          269 a          243 c          227 g          240 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagg
//
LOCUS       DAJTNW010000271          953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNW010000271 DAJTNW010000000
VERSION     DAJTNW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..220)
                     /locus_tag="OO179_004258"
     CDS             complement(<1..220)
                     /locus_tag="OO179_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5534075.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGIST"
     gene            697..933
                     /locus_tag="OO179_004259"
     CDS             697..933
                     /locus_tag="OO179_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534076.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          268 a          233 c          210 g          242 t
ORIGIN      
        1 gggtggaaat accggtcact cgagaacccg gccagtcttg cgactgccct gcacatccca
       61 ccataatttg aatggagctg tgcattacgc ataaaaaaac cgcttcagcg cggctatgcg
      121 ctcgagtaac gttcgggttt ccacgcccgg cccccgtttt atgaggtgca gatgcactat
      181 aattccaccc gttctggttt tcaatagcta cattcaacat tttctcttac ctttcatcac
      241 cgaagtgaac tttgttgatg cggtgcctgg tgcctccagg tgacgttaac cagttaacaa
      301 ttaacgccgg atacagggaa acccataacc acccgatacg tgggaactaa tcgcttttta
      361 actgttccgc gtgcgctcag ccgcattcac cgcatcacaa aattcacttt aaaaagggcg
      421 gacatcagcc agcaatgaaa ctgatgccgc caaaggtacc aatcaacatg gagtgttgtg
      481 gcgtggttgt cacttaagcg tatggtcaac ctgacaaccc ggtgtcctca acggggaagg
      541 aataactccg ccatacttac cgccgcgtca tttcgcgttg tgtgcctgct tttaactacg
      601 tcaggcgagg tggttcctgt tattccccaa caacaagaaa tttgtataat ctgaataccc
      661 caacaataag aagagtgagc aagtgatgta ttagcgatgt cagctctcaa agagactgca
      721 tcaatgttaa aattattcaa tgaagcaaaa actcaggctg aggttgatcg cgctgtgtct
      781 gaaataaact ctaagttgtc aagcattcag cttgaatatt tgtctctcat tgatgagctg
      841 acaatctcta aaagcaatga ggctgcgctc aaagcaaaac tcacggagat agaaaagttt
      901 aagttccaaa gtgaagactg cgaacctcgc taattgatta ctggaacgat tgt
//
LOCUS       DAJTNW010000272          906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNW010000272 DAJTNW010000000
VERSION     DAJTNW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            12..557
                     /locus_tag="OO179_004260"
     CDS             12..557
                     /locus_tag="OO179_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5534077.1"
                     /translation="MLHLKNITTGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            581..>906
                     /locus_tag="OO179_004261"
     CDS             581..>906
                     /locus_tag="OO179_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5534078.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEE"
BASE COUNT          264 a          192 c          236 g          214 t
ORIGIN      
        1 agtatttaac aatgttgcat ttaaaaaata ttactacggg taatccgaaa acagcagaac
       61 aatatcagat gacaaaacga tattcggtca cctggctttt ttcagaagac ggaaaaaact
      121 ggtatgaaga gctgaagaat ttcgccagcg acacaataaa aatagcttac accggagatg
      181 gtcgcgtggt gtgggtcggt aaggatgtga caggcatcga gccacgcaat gccagcgtta
      241 tcgaagttcc ggatattacc actaaccgac ggattaccgc gccgggttac tggttttacc
      301 gcaatgatga atttgtcttt gactacagac tcaaagcgga agatgagcgt gatgcccttc
      361 tggctcaggt cagtgcccgg acaggggaat gggaagaaga cctgctgctg gggttaatca
      421 gcgacgaaga taaagaaaag ctgaaagcct gtcgtattta cgcgaaatcg ctgcaggcga
      481 tggatttcag caccatcact gataaatcct catacaacgc cattgaatgg cccgcctctc
      541 cggaaggttc ttcctgattt aatttatcgc gagaaaaaca atgtctgtag tgatatcagg
      601 tgcgctgact gatggcgcag gtatccccat gtccggatac catattattc tgaaatcccg
      661 ggtaaacacc ccggaagtgg tgatgaacac tgttgctgat gtgatgacag gaaacgatgg
      721 tgaatactgt ttccatgcgc ggactggaaa atatggtgtg tatctgaaac aggactggcg
      781 caacgagtac aacgttggcg acattgctgt atatgaggac tcaaagcccg gcacgctgaa
      841 tgactttctg attgctcctg atgagggcga cctgaaaccg gatgtcgtca aacgctttga
      901 ggaaat
//
LOCUS       DAJTNW010000273          904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNW010000273 DAJTNW010000000
VERSION     DAJTNW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..269
                     /gene="tnpB"
                     /locus_tag="OO179_004262"
     CDS             <1..269
                     /gene="tnpB"
                     /locus_tag="OO179_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5534079.1"
                     /translation="SKVQNVLKDDPFSGHLFIFRGRRGDQIKVLWADSDGLCLFTRRL
                     ERGRFVWPVTRDGKVHLTPAQLSMLLEGIDWKHPKRTERAGIRI"
     gene            289..>904
                     /locus_tag="OO179_004263"
     CDS             289..>904
                     /locus_tag="OO179_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5534080.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVVKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADY"
BASE COUNT          211 a          258 c          253 g          182 t
ORIGIN      
        1 cctcaaaagt tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc
       61 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
      121 ttaccagacg cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc
      181 accttactcc ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac
      241 gaacggaacg cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
      301 cttgctcatg agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
      361 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
      421 aaccggctga aggcgctggt tgtgaaactg cagcgcatgc agttcggtaa aagctcagaa
      481 aaacttcgcg caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt
      541 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg
      601 cgccagtctt cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc
      661 cgtccggaag aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat
      721 gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
      781 aaactggcct gttgccggtg tgaccatatc gtgcaggcac cggtgccttc aaaacccatt
      841 gcacgcagtt atgccggagc ggggcttctg gcccatgttg ttgctggaaa atatgcagac
      901 tatc
//
LOCUS       DAJTNW010000274          901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNW010000274 DAJTNW010000000
VERSION     DAJTNW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..87
                     /gene="hokD"
                     /locus_tag="OO179_004264"
     CDS             <1..87
                     /gene="hokD"
                     /locus_tag="OO179_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5534081.1"
                     /translation="VTRKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            305..520
                     /locus_tag="OO179_004265"
     CDS             305..520
                     /locus_tag="OO179_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534082.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            623..901
                     /locus_tag="OO179_004266"
                     /pseudo
     CDS             623..901
                     /locus_tag="OO179_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          243 a          215 c          224 g          219 t
ORIGIN      
        1 gtaacgagga aagacctctg cgaggtacga atccgaaccg gccagacgga ggtcgctgtc
       61 ttcacagctt acgaacctga ggagtaagag acccggcgag tgagaaatcc ctcgccacct
      121 ctgatgtggc aggcatcctc aacgcacccg cacttaaccc gcttcggcgg gtttttgttt
      181 ttattttcaa cgcatttgaa gttctggatg gtgccggaat agaatcaaaa atacttaagt
      241 agcgcgcagg gataaaaggg atggaccccg aacaggggag tgctacttat ctggaaggat
      301 tctgatgatg aacatcgaag aactgcgtaa aattttttgt gaagatggcc tttatgctgt
      361 gtgcgttgaa aatggaaata ttgttagtca ttaccgcatt gtgtgtttgc aaaaaaatgg
      421 ggctgcgtta attaattttg tggatgcccg agtgacggac ggatttatct tgcgcgacgg
      481 tgagtttgtc acttcattac aggcattgaa agagatctga ataaaagctg gcttttctgc
      541 tttttcagaa gaataaactc atctacaatc ttgcgcgggg ctgaactccc gctgagtaac
      601 accgtgccac cggagaaaac cgatggcacg caacgcaaaa tattacaatc atgataattc
      661 gaccgttctt gcccacacgc acgagcggta ttctcacgca tttaagtcag actggtacca
      721 gcatccctca tgcactgaag aacaggccga atggcttatt cagtgttacc gcaggcgcgg
      781 atgcgaggtt aaaaaagccc ttagcctcga ctaccgtcac tagataatct ccgtcaggct
      841 cccttactcc gaacagccac cgcgtccgtc ccgcacattc cagcaacgga tctggaggta
      901 a
//
LOCUS       DAJTNW010000275          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNW010000275 DAJTNW010000000
VERSION     DAJTNW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            170..382
                     /locus_tag="OO179_004267"
     CDS             170..382
                     /locus_tag="OO179_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5534083.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            626..865
                     /locus_tag="OO179_004268"
                     /pseudo
     CDS             626..865
                     /locus_tag="OO179_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
BASE COUNT          259 a          171 c          253 g          212 t
ORIGIN      
        1 ataaaggcag aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc cccagagaga
       61 ggaggcaggg aaataaggct taaaaggaaa taacttccgc ttccgaaagg aagccagtcc
      121 actgaattat ttatcacaat aatactgaca actaaagggg gacaagaaaa tgaatggatt
      181 cagaaacagt tcacggaacg gccaggtctg gcgttaccag cgtgccggcg ggcgggccgt
      241 tattctggaa gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgcctg
      301 tgttgcgcct aggacagact ggcagcagtt tgcccggaaa agggctgaac actgccaccg
      361 gcgctgccgg ggaagggtgt aacgaaaaaa gcagccttca ttactctagg agtcagaagg
      421 ctgaaaggag tttatgagat ttgcttctta atatccggtc gggcagtatg accggacagt
      481 tctgagtagc acagagtctt ttccgcatta agtcgatttt ttatattttg tgattagttt
      541 cacaaaataa ggtgttattc aatgtgtgct gcactattca ggggggctga aatacaggtg
      601 gcatttatct gatatggaga ataacatgag gaaatatatt ccactggtat tatttatctt
      661 ttcatggccg gtattgtctg cagatattca tggacgggtt gttcgtgtac tggacggtga
      721 taccatcgag gtcatggact cactgaaggc ggtgcggatc aggctggtaa atattgatgc
      781 accggaaaag aaacaggatt atggccggtg gtcaacagac atgatgaaat cactggtcgc
      841 aggaaaaacg gttaccgtca catattgagg tgtactggca atagcggaca ctacc
//
LOCUS       DAJTNW010000276          883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNW010000276 DAJTNW010000000
VERSION     DAJTNW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..552
                     /locus_tag="OO179_004269"
     CDS             <1..552
                     /locus_tag="OO179_004269"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534084.1"
                     /translation="GDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQ
                     AIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGL
                     TGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNE
                     VETALFNARALALKIYSMSGWNL"
BASE COUNT          252 a          163 c          218 g          250 t
ORIGIN      
        1 ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag aactcagcat
       61 gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga tggtgtgaca
      121 gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc ctccggcggg
      181 gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc cactttgctt
      241 attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg agcaaaaagc
      301 ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg ccgttatctg
      361 agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa cattacagca
      421 gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat ggtgaatgaa
      481 gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag catgtcaggc
      541 tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta gtgtcagctc
      601 ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca ggattcagcc
      661 tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt tagacagtga
      721 gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg atttgcatat
      781 ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga atgtaaacgg
      841 aggattttct ttctcaaact catggatagc agcagatgcc gat
//
LOCUS       DAJTNW010000277          874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNW010000277 DAJTNW010000000
VERSION     DAJTNW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(100..501)
                     /locus_tag="OO179_004270"
                     /pseudo
     CDS             complement(100..501)
                     /locus_tag="OO179_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          226 a          204 c          185 g          259 t
ORIGIN      
        1 atactttttt cgtaagcgat gagatgtgga gccagaaaaa tggctgcgtt acgtcattga
       61 actgtccttg acccacgata ttcagacagt cgttgtatct taagttgaca ttacccgctg
      121 acgtcgatat tcccttggtg agagataacc caacgcacta tgcggatggt tttcattgta
      181 atgctcgatc gccactgcaa ggttatgcaa tgccgttctt ggtttcggca tgaacgcgat
      241 gcagtcttcc ttcatcgttt tcatgaaccg ttctgctatt ccattactct gagggctact
      301 gactgccgtc gtgcagggct ccagattcaa ctctctggcg aactgccgtg tttcatgagc
      361 cgtataagcc gaaccgttat cggccactgg attggccgtt ccggtccttt atcgccatat
      421 cgctcctgac atgacatcct gcaccgttgc tttgtcataa tctcccgtgc ctgctgccca
      481 gtctatggct tcacgatcgc aatatggaac gtaacgctgt catcgggaga atcgtcaatg
      541 cggcattcgg ttaacgctta ccaatatcaa caccaaccgg atttgtggag cctccagtct
      601 gaaggggctt tatgcgttcc gtataggata ttatgatgct ggagttttgc gaagctccac
      661 tcctttccga acatttacac agatattgcc taagaaaagt gaaaccataa tatggttata
      721 gtccctttca gtgcaaatac ttagcttgtt gcacagagaa atagaagctg cataagctct
      781 ttcttcaaaa aatgtaaata aagttaaata taggaaaaat tacttaatct atcttaataa
      841 cggaaagatt ttgtatacaa tcactgttac agaa
//
LOCUS       DAJTNW010000278          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNW010000278 DAJTNW010000000
VERSION     DAJTNW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..254)
                     /locus_tag="OO179_004271"
     CDS             complement(<1..254)
                     /locus_tag="OO179_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534085.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="OO179_004272"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="OO179_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5534086.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="OO179_004273"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="OO179_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5534087.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          239 a          174 c          219 g          229 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg a
//
LOCUS       DAJTNW010000279          856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTNW010000279 DAJTNW010000000
VERSION     DAJTNW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..152
                     /locus_tag="OO179_004274"
     CDS             <1..152
                     /locus_tag="OO179_004274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534088.1"
                     /translation="VRVDDEGYPEIARCMEREFATIPAGINADGMYCPECGTVHWPDG
                     VIPPF"
     gene            200..629
                     /locus_tag="OO179_004275"
                     /pseudo
     CDS             200..629
                     /locus_tag="OO179_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          217 a          169 c          267 g          203 t
ORIGIN      
        1 ttgtacgtgt tgatgacgaa gggtatccgg aaattgcccg ctgcatggag cgggaatttg
       61 cgacaattcc tgccggtatc aacgccgacg gcatgtattg ccctgaatgc ggtacggttc
      121 actggccgga tggcgttatt ccgcctttct gatgcttccc cgttttgccg atatttttca
      181 gcagggaaac cgatggctta actggctgga gaagcagccg gtgcagatga gccgcctgga
      241 acattatgcc ggtcaggatg aaattggcct gcgttacaac agccaccgga caaaacgaga
      301 agaaaacctt gtgatgagtg gcgacgagtt tatggaaagg ttctcctggc atgtggcgga
      361 taaggggttc cggatggtga tacggggtcc tgagtccggt gaagcggcga ttactggaag
      421 atgtggtgta cgccataacg gagacagtga gaaaaacggc gaggcaaatc acaaggagag
      481 ggatgtatca gcagtgactg aaggttgacc cgctgaagtg cgagctgcgc ggaggtcaga
      541 tgcggtttac ggggctgaag aggggttacc gtctggcaga gcaggttatg atgcatgagc
      601 cgctggctcg aatacggtgg tgcggctgag agtcgcagag gggaagttgc gtccatgttg
      661 aggggaacgg agcaaaaaac catcagagat acattgaatc aatcaatgta agcaatatta
      721 agtgtctata ccgattactt gatgatcagg ttgtgttgaa taaacgcgca ttcttcggtg
      781 cttttttaat cttttgggga tttaaattcc tatcgatgaa ctgtcgatat gaaaaagcga
      841 gcatcatact gacgtc
//
LOCUS       DAJTNW010000280          846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNW010000280 DAJTNW010000000
VERSION     DAJTNW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>846)
                     /gene="capU"
                     /locus_tag="OO179_004276"
     CDS             complement(<1..>846)
                     /gene="capU"
                     /locus_tag="OO179_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5534089.1"
                     /translation="NSKIAFEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAI
                     VCHSGHDSNIVGLVRLFTRKHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTH
                     LEQEGCRTRVTVVPPGFDFQKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKG
                     HEFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAP
                     VYRVASLVVLPSENESFGMVLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKH
                     AWMCAL"
BASE COUNT          254 a          207 c          166 g          219 t
ORIGIN      
        1 cagggcgcac atccatgcgt gcttattacc tgctggtaac aatgtcccgg tctggttatt
       61 ctgaataaca tcagggattc caccaatctg actggccagt acaggcacag aaaatgccga
      121 tgcttctgcc agtaccatac caaacgattc gttttctgaa ggcagaacca ccagactggc
      181 aacccgatat acgggggcgg caggaaaaac attgtcagca ataaaaacat catcatgcat
      241 gcccatactg tcaatctgat actgtaaatg ctcccgcagt tcaggcgaac cagaccccac
      301 aatcaaccaa cagaactgtc gtccattcat ttttaaatgg aacagtaaat tcaacataaa
      361 ttcgtgccct ttttccgggc gcaacattcc tacctgagca ataacggggc atccccttcg
      421 ggacgccagc caagaaagaa catttggagg caaactgttt cgggaatcaa cgtataattt
      481 ctggaaatca aagcctggag gcacaacagt aacccgggtt cgacatcctt cctgctccag
      541 atgtgtcttc atacttgttc cgggaacaat cacctcatcg caaaaatgat ttattgaaaa
      601 aacttttgtt tttcgtgtca aatatgtctt ttgcctgata attctgaatg gatgtttcct
      661 agtgaataac cgtactaaac caacaatatt gctatcatgc ccactgtgac aaacgattgc
      721 attgggctga aaaccatgaa ctattccgag taatctccat gcagtaggga tgtgaagact
      781 gtttcgaaat aacgcgaatg tgatatcaat ccccaattta ctggcttcaa aagcaatttt
      841 gctgtt
//
LOCUS       DAJTNW010000281          811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNW010000281 DAJTNW010000000
VERSION     DAJTNW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>811
                     /locus_tag="OO179_004277"
     CDS             <1..>811
                     /locus_tag="OO179_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5534090.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJTNW010000282          780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNW010000282 DAJTNW010000000
VERSION     DAJTNW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..77)
                     /locus_tag="OO179_004278"
     CDS             complement(<1..77)
                     /locus_tag="OO179_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HCR5534091.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVT"
     gene            complement(106..332)
                     /locus_tag="OO179_004279"
                     /pseudo
     CDS             complement(106..332)
                     /locus_tag="OO179_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(329..598)
                     /locus_tag="OO179_004280"
                     /pseudo
     CDS             complement(329..598)
                     /locus_tag="OO179_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          192 a          209 c          169 g          210 t
ORIGIN      
        1 tccgttacta tgccagcatg agaattatta ttctcaaatg gaaaaggtgt ttgaataatg
       61 gtagatgtta ttttcatgtc atacctttgt tacgatattt aaaaatcaca agagattttc
      121 acacaaaata acgttacacg ctaccttgtg tggtccagat gttccttcca cacgaccgtt
      181 gcggccttta tacggctgcc ctgccaggct gctggctgaa taaccaatcc gttcgtccgg
      241 ttcaatggcg gccatcaggt tctgctccgc aatgtactac gaatgcgctt cctgtcctgg
      301 atggccagca tggtccagaa catctccatc atttatcagc aatcttcctg ctcatttcat
      361 cccgccatgc agcagcagag gattcgacca tgtcatgggg aagcacatca ccgcattcga
      421 gttgctgacg gcctgccgca acctagtcac ggaaccaaga attgtgctgc cgttcaacgg
      481 tctggcgcat aaaatcccgg attaactgag agccattacg gtccatgctt tttgcagcgg
      541 ccataaaagc atccttcagt tctgcgtcga ttcttacact catattaacc tgtgccattt
      601 gccacctcgt cattaccacg tatatacaca gtatataact gttcagatat cgtcaccagg
      661 atatgccgcg ccagcggcat gtaaggcggc actccgttgt tgcatatggc agaggagtaa
      721 aaccgccgaa gcccggcgta agccggtacg tattgacagg tttcacctgt cctatcactg
//
LOCUS       DAJTNW010000283          777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNW010000283 DAJTNW010000000
VERSION     DAJTNW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            44..391
                     /locus_tag="OO179_004281"
     CDS             44..391
                     /locus_tag="OO179_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5534092.1"
                     /translation="MKIRLRVDEPTADFGTEPTYPESFDVWAKVAQPGAAAYQGSVQT
                     ENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIRDLNGQRRFLLLECEELRTARRRG
                     ECHESDSIFTRRL"
     gene            357..>777
                     /locus_tag="OO179_004282"
     CDS             357..>777
                     /locus_tag="OO179_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534093.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRN"
BASE COUNT          183 a          197 c          217 g          180 t
ORIGIN      
        1 tgccacttac cttttaccgg atcccggaga gctgaatcgc cggataaaga tccgccttcg
       61 tgtggatgaa cccaccgctg attttggtac ggagccaacg tatccggagt cgtttgatgt
      121 ctgggcaaag gtggctcagc caggcgctgc cgcttatcag ggctcagtgc agacagaaaa
      181 tatcgttacg cattatttta cgatccgttt ccggcacgac atcacggcag atcacgaagt
      241 ggtttattac ggccaagagt accggatccg gcgaatacgt gacctgaacg gtcagcggcg
      301 ttttttatta ctggaatgtg aagaactgcg tacagcgcga cgacggggtg aatgccatga
      361 atcagacagc atttttacac gtcgacttta aacaaccaga ggagctggag tttaatcgcg
      421 cccgtctgcg tcgggcgttt gtgcaaatcg ggcgcgttta catgcgtgat gccagacgtc
      481 tggtgattaa acgcggacgc tccggtccgg gagagaaccc gggatatcag accgggcgtc
      541 tggcccgctc cattggttat tacgttccca aaaaaacgac gcgtcgcccc ggactgatgg
      601 tgaaaatttc ccctgaccag aaaaacgggc aggggaatcg ccgttttcct gaaggtgctc
      661 cttattatcc ggcgtttctg tattacggcg ttcgccattc agcgtatgga atggataaaa
      721 aggataagcg gcagaagaag caccattctt cgactttccg gctggccccg cgtaata
//
LOCUS       DAJTNW010000284          748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNW010000284 DAJTNW010000000
VERSION     DAJTNW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..574
                     /locus_tag="OO179_004283"
                     /pseudo
     CDS             1..574
                     /locus_tag="OO179_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            578..748
                     /locus_tag="OO179_004284"
                     /pseudo
     CDS             578..748
                     /locus_tag="OO179_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          181 a          192 c          222 g          153 t
ORIGIN      
        1 cgtggagctg agccgtgcca cactggggcg ctggacaggt gctgttgctg aactgctgga
       61 accgctgtat gacatcctgc gccagtatgt gctgatgccc ggtaaagtcc atgccgatga
      121 tatccccgtt ccggtccagg agccgggcag cggtaaaaca aggactgccc ggctgtgggt
      181 ctacgttcgt gatgaccgta acgccggttc ggaaatgcct ccggcggtct ggttcgcgta
      241 ctcaccggac cggaaaggta tccatccaca gaatcatctg gccggttaca gcggagtgct
      301 tcaggccgat gcttacggtg gttaccgggc gttgtacgaa tccggcagaa taacggaagc
      361 cgcatgtatg gctcatgtcc ggagaaaaat ccacgatgtg catgcaagag tgccaaccga
      421 catcaccacg gaagccctgc agcgtatcgg tgaactgtat gccatcgagg cagaagtccg
      481 gggatgttca gcagaacagc gtctggcggc aagaaaagcc agagccgcgc cactgatgca
      541 gtcactgtat gactggatac agcaacagat gaaaattcat tcgctgaaaa tggaatgtct
      601 ccacggagaa cactattatc catcgggaaa tagtgcgggc aacagtgttt agttatatcg
      661 agtgcgatta caatcgctgg cgacggcacc gttggtgtgg tggtctcagt ccggaacaat
      721 ttgaaaacca gagcctcact tacgactg
//
LOCUS       DAJTNW010000285          748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNW010000285 DAJTNW010000000
VERSION     DAJTNW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>748
                     /locus_tag="OO179_004285"
     CDS             <1..>748
                     /locus_tag="OO179_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5534094.1"
                     /translation="TDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELDLEA
                     SNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFM
                     TNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSF
                     AGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNK
                     TQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          209 a          152 c          181 g          206 t
ORIGIN      
        1 gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt atgcccgtgt
       61 aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc agtttgaact
      121 agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat atgctggttt
      181 gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg cgtatgatgc
      241 agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg gtactgacct
      301 gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt tctttggcat
      361 ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta caggcaacta
      421 caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg gtttctcatt
      481 tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg gtaaaggcga
      541 aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt atgctgctgt
      601 gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga acaaaacaca
      661 aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat ccatcggtta
      721 ctcctattct aaagcactgg acgtagca
//
LOCUS       DAJTNW010000286          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNW010000286 DAJTNW010000000
VERSION     DAJTNW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>747
                     /locus_tag="OO179_004286"
     CDS             <1..>747
                     /locus_tag="OO179_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR5534095.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAJTNW010000287          742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNW010000287 DAJTNW010000000
VERSION     DAJTNW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(1..355)
                     /locus_tag="OO179_004287"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="OO179_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(470..>742)
                     /locus_tag="OO179_004288"
     CDS             complement(470..>742)
                     /locus_tag="OO179_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5534096.1"
                     /translation="RWPGWLERVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYG
                     RKWSGITPEKFMQQVDAYIRWYNERRIKLSLGAVSPKMYRQQCGLE"
BASE COUNT          162 a          210 c          191 g          179 t
ORIGIN      
        1 attcggccat gcgctccgaa gcctgctgcc agagtggagc gtgatgctcc ggcacggtct
       61 gccagccgta tgcagccgaa cagagacggt gcgatgctac ctgttgctca ttactgtaga
      121 tccgcagttc atcatccagc gagatccgta ttgataccag ctgaccacac aggctttccg
      181 gcacactgta acggttcccg ccaacctcga tatagccatc ccaggagaca tgacggatat
      241 cgaagtccgt acccagcagc ggctgcagat gctcctgttc ctgcgtgaag cgctgttccg
      301 gtgtctgcct gaactggcga agttcccgcc tgtcggcaac gtcagccatc cactgcgcag
      361 catgagagtt tcctttgtgg tgtaggaacc ttaaggaaac aacatgttgg gtggagcgga
      421 caatccaaat ggtgaagggg ggatgcggac gatttcctgg actgctttat cattccagcc
      481 cgcattgttg gcggtacatt ttggggctga cggcacccag cgataatttt atacgccgct
      541 cgttatacca tctgatgtaa gcatccactt gctgcatgaa cttttctggc gtgatgcccg
      601 accatttacg cccataatac atttccgttt tcagtctgcc gaaaaagcct tcgcatgcag
      661 cattatcagg tgaacatcct ttacgggaca ttgagcgaat aagacctgct gcattcactc
      721 tttccagcca gcctggccag cg
//
LOCUS       DAJTNW010000288          735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNW010000288 DAJTNW010000000
VERSION     DAJTNW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..588
                     /locus_tag="OO179_004289"
     CDS             <1..588
                     /locus_tag="OO179_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5534097.1"
                     /translation="ELINLSLEDAIMATIPALSHPDVTIENGRAVTTSVAVAEFFHKR
                     HDNVLRAIANTECSPEFNALNFEDVTYTDTKGEKRPMYQITKNGFVFLVMGFTGKKAA
                     AFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVVHFDKFGNVEFTETVPDGALV
                     CTLETFRFYLEKQGWTLVNRGAIKNMTVEQLLSLK"
BASE COUNT          212 a          191 c          150 g          182 t
ORIGIN      
        1 gagttaatta acttatcact cgaggatgcc atcatggcta ctatccctgc cctttctcac
       61 cctgacgtaa ccatcgaaaa cggtcgcgct gtcactacgt ctgttgcggt tgcagagttc
      121 ttccacaaac gacacgacaa tgtgttacgt gccatcgcaa atactgaatg ctcacctgaa
      181 tttaacgccc tcaattttga ggacgtcacc tacaccgaca ccaaaggcga aaaacgccca
      241 atgtaccaaa tcaccaaaaa cggcttcgtt ttcctggtga tgggcttcac tggcaaaaaa
      301 gccgccgcat tcaaggaagc ctacattgct gagttcgacc gcatggaggc cgaactgcgc
      361 cagaataaca ccccacctgc tgacaagatg attccgggtg atggtcgcac tctggttgtt
      421 cacttcgaca aattcggcaa tgtcgaattc accgaaaccg ttcctgatgg cgcactggtc
      481 tgtaccctgg agactttccg gttttatctg gaaaaacagg gatggactct tgttaaccgg
      541 ggcgcaatta aaaatatgac tgtggaacaa ttactttctt taaaataaaa atggagctac
      601 ggatatgaac cctatgcttg attattgttt tacctctgca cctcagcaga ccataacaca
      661 caccggaatc aaaaatttgt tatggataat caaaaaaaac ctccagcaga aaggtgatcc
      721 cgatttaatt ccctg
//
LOCUS       DAJTNW010000289          711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNW010000289 DAJTNW010000000
VERSION     DAJTNW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            307..468
                     /locus_tag="OO179_004290"
     CDS             307..468
                     /locus_tag="OO179_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HCR5534098.1"
                     /translation="MLYDKESESLFPIDFRNIYAEYYAATKKDKEIIDRRLQMRTNDF
                     YSLLNRKYL"
BASE COUNT          227 a          130 c          148 g          206 t
ORIGIN      
        1 tttgaagatg tgaatgattc atctgctttg tataaaaagt atgatcttat tggcaaccag
       61 tacaatgaga ttctggaaat ggcttggcaa gaatctgagc tttttaatgc tttttatggc
      121 gatgaagcat ccgttgttat acagtatggc ggagatgtgt acctccgaat gctgcgcgtg
      181 cctgggactc cccttagtga cattgataca gctgatatcc ctgataatat agagagcctt
      241 tatctacagt tgatatgtaa attgaatgag ttgagtataa tccattacga tcttaataca
      301 ggtaatatgc tgtatgataa agaaagtgag agtttattcc caatagattt tcgcaatatt
      361 tatgctgaat attacgctgc aaccaaaaaa gataaagaga ttatcgaccg acgattacaa
      421 atgcgtacaa atgattttta ttcgttatta aacaggaaat atttatagac gtatttgttg
      481 atgctataag ccagaggctc ttctatgaga atatggggac cattccctcg cgatattaac
      541 gtaatatctt aattgtatgc accgccagaa tgcgttcagt gcgatcacgg cagcagacag
      601 gtaaaaatag caacaaacca cccgaaaaac cgccgcgatc gcgtatgata aattttaacc
      661 acatgcatac ctgtgcagca aggcgaatca cgcacggatt tccagccaga g
//
LOCUS       DAJTNW010000290          704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNW010000290 DAJTNW010000000
VERSION     DAJTNW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(101..457)
                     /locus_tag="OO179_004291"
     CDS             complement(101..457)
                     /locus_tag="OO179_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534099.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDLH"
     gene            complement(461..>704)
                     /locus_tag="OO179_004292"
     CDS             complement(461..>704)
                     /locus_tag="OO179_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534100.1"
                     /translation="FKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKG
                     RNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          156 a          193 c          183 g          172 t
ORIGIN      
        1 agcatcaccg gcacctgtga attcagcgtt ggtcagcacc acgtttttgc cactgactga
       61 cgtggacatg aatccaccca gaggcacttt gatggattca tcagtggaga tcgccttttt
      121 tgatagtgct ggcatcaaaa tcagaaccga gattaacggt cacgtagcca cgcttcatgg
      181 cgtcgcccgg gaagttcttg cctgttccca cctggcgaac catgtccggc tgcgacgtgg
      241 tcggataagg gcgcacgtag atccccttca ccttgtctgc ggtatcacca tctgccagcg
      301 gcacgaaaaa accgtcatca tcgtatttac cagccaggcc ataggcagcg aaggcgttat
      361 cggatttaag gaccaccggt tcgacggtta agtcctgcgg gcgagagaca gccccggcaa
      421 tgccaacagg catccggtac agaaatacat tattcatttt ttacccttta cggtttgccc
      481 agaattcagc gttttgtttg ttcagggaag cgatactggt catgcccatg tttaggcgct
      541 gtgcatcgcc ggtggtggcg cgggtgtttc gccctttggc aacctcagac acggcattaa
      601 acgccatgtc gaccgattgt ttcggtaatt tgcggatatc cgcatcaccg actatctggc
      661 gaaccagcgt tttgtcagca gaagccagaa cctcgcgttt gaac
//
LOCUS       DAJTNW010000291          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNW010000291 DAJTNW010000000
VERSION     DAJTNW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>693)
                     /gene="ipaH4.5"
                     /locus_tag="OO179_004293"
     CDS             complement(<1..>693)
                     /gene="ipaH4.5"
                     /locus_tag="OO179_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5534101.1"
                     /translation="QCTRDYHRIWDDWAREGTTTENRIQAVRLLKICLDTREPVLNLS
                     LLKLRSLPPLPLHIRELNISNNELISLPENSPLLTELHVNGNNLNILPTLPSQLIKLN
                     ISFNRNLSCLPSLPPYLQSLSARFNSLETLPELPSTLTILRIEGNRLTVLPELPHRLQ
                     ELFVSGNRLQELPEFPQSLKYLKVGENQLRRLSRLPQELLALDVSNNLLTSLPENIIT
                     LPICTNVNISGNP"
BASE COUNT          201 a          111 c          164 g          217 t
ORIGIN      
        1 tggattccct gaaatgttaa cattcgtaca aatgggcaat gtgattatat tttcgggtaa
       61 tgaagttagt aggttattgg aaacatccag tgccaatagt tcttgcggta atctggataa
      121 tctgcgtagt tgattttcac ctaccttcaa atattttaag ctctgaggaa attctggtag
      181 ttcctgtagt ctgttgccgg aaacaaagag ttcttgtagt ctatgaggca attcaggcaa
      241 gacagtaagg cgattacctt caatacgtaa tattgttagc gttgatggaa gctctggtaa
      301 cgtctccaga ctattaaaac gtgccgagag tgattgtaaa tatggtggta atgatggcag
      361 acatgacaaa tttcgattga atgaaatatt aagcttaatc agttgagatg gaagtgtcgg
      421 gagtatattc aagttgttac catttacatg aagttctgtc aaaagcggag aattttcagg
      481 tagggagatt aactcattgt tggaaatatt aagttcacgt atatgcaaag ggagtggtgg
      541 taaagaacgt agtttcagta agcttaaatt gagaacaggc tcccgggtat ccagacatat
      601 tttcaataat cgaaccgcct ggatgcgatt ttctgttgtt gttccttccc tagcccagtc
      661 atcccagatg cggtggtaat ctctggtaca ctg
//
LOCUS       DAJTNW010000292          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNW010000292 DAJTNW010000000
VERSION     DAJTNW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          215 a          168 c          134 g          169 t
ORIGIN      
        1 acttaatagg ggcccccccc gacagacctg tgctatcgga aagaaaaaag gccatcagca
       61 aaactctgat gacctgaatc acctttacca atattgtatg aaaaaacacg cacgactaat
      121 tgacaataat tttcatttcc attaaaaaag atcgcgtagc attcttaatt catgaggaac
      181 tttacccgcc agcaatctga gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg
      241 tccttttttc tacggtcctc ctccccggag gggggcattt cattattttt ctaatcgtta
      301 ctggtgagaa ggcggaagaa aaagccagtt cttcgcccct aaataacagc atgattccag
      361 tccatgcaaa atctgatttc cagctcacac ataccgagcg ctgtacaaaa aaccgccagc
      421 caaggcagtt aagagtgtgt tcccggggtt tgcttagaat attttgtaag ttgtccgaag
      481 agatatttac aacaccagaa tgatgaatca tcagtcccct gccagaggtt cactacgtaa
      541 cctgaccgac aatgcaccat tctggtgctg aaaaaagagc actggaactg caacaaaaca
      601 acatcacaga acaggagatg gtaaggagtc agacattccc acacaatatt gtgtcagtgc
      661 aaataacaac cttcgtctca aatctc
//
LOCUS       DAJTNW010000293          673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTNW010000293 DAJTNW010000000
VERSION     DAJTNW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(1..485)
                     /locus_tag="OO179_004294"
                     /pseudo
     CDS             complement(1..485)
                     /locus_tag="OO179_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            483..673
                     /locus_tag="OO179_004295"
                     /pseudo
     CDS             483..673
                     /locus_tag="OO179_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          136 a          206 c          185 g          146 t
ORIGIN      
        1 ctcggtccga gtgaacgata acgttccggg gtctcttacg ccgccacagc gccatctgca
       61 gggcatcgca ggccagttgc gccgtcaggc gtggtgacat ggaccagcca ataacggcac
      121 gtgaccacag gtcaatgacc acagccagat acagccagcc ttcatctgta cgtaagtacg
      181 tgatgtctcc cgcccacttc tggttcggac cactggcgta aaaatcctgc tccagcagat
      241 tttctgacac gggcaggcca tgtgcgcggt aactgaccgg gctgaacttc cgggaggcct
      301 ttgccctcag cccctgacgg cgcaggcttg ccgccacggt ttttacgtta aagtggtaac
      361 cctgagcacg cagttcatcc gtcaggcgtg gtgcaccgca acgctgtttt gaccgggtaa
      421 agaacgctgt cgcagtgctg acggaactgc tgacgagggc ttatccctgt ccgccgctga
      481 caccacgttc aacagtttgt ggactgttcg ctcgcttccg gaaatcaggg ctttcatggc
      541 ctttgcctgc aggtatgtcg gagcaggagc ttgatgccct tctttacgga agtgcctcca
      601 cagtacctgt cgtacttact gaaagcaccg ttatgcccaa actcccggtg gtaaaaaaac
      661 gtccccggcg gcc
//
LOCUS       DAJTNW010000294          660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTNW010000294 DAJTNW010000000
VERSION     DAJTNW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            55..276
                     /gene="kilR"
                     /locus_tag="OO179_004296"
     CDS             55..276
                     /gene="kilR"
                     /locus_tag="OO179_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCR5534102.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            270..446
                     /locus_tag="OO179_004297"
     CDS             270..446
                     /locus_tag="OO179_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534103.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            521..>660
                     /locus_tag="OO179_004298"
     CDS             521..>660
                     /locus_tag="OO179_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534104.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGP"
BASE COUNT          223 a          121 c          132 g          184 t
ORIGIN      
        1 ttaaataact ttcctgagat ttcctttgta agactgttta ttaaggaggc ttccatgatt
       61 gctcatcact tcggaactga tgaaatacca cgtcagtgtg tgactcctgg cgattatgtt
      121 cttcatgaag gtcggacata tatcgcctcg gcaaacaata ttaaaaagcg aaaactttat
      181 attcgtagcc tgactacaaa aacatgcatt tctgactgca tgattaaagt cttcctcggt
      241 cgtgatggtt tacctgtaaa ggcggagtca tggtaatgac taagaaaata aaatgtgctt
      301 atcacctttg caataaagaa attgaagaaa gcaaaatcat tacaagacca cttcatttca
      361 tgcgtggagt tataccaacg acggaaatga aaaaatattg tagtgaaatc tgtgccgaaa
      421 aagaccagat ggcacacgaa ctttaattaa ctgactattc gaaactgaat ttatgccagc
      481 aatggcaggg attcgctcaa ccttaattaa ggagaaaaac atgattacca gttatgaagc
      541 cactgttgta actactgatg acattgttca cgaggttaat ctggaaggaa agcgtattgg
      601 ctacgtgatt aagacagaaa ataaagaaac cccattcact gtggttgata tcgacggtcc
//
LOCUS       DAJTNW010000295          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNW010000295 DAJTNW010000000
VERSION     DAJTNW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          181 a          117 c          141 g          200 t
ORIGIN      
        1 tgttttattg ttgcaacttg cgtaattatt caggtcagtt tggaacgcac tgtataaaca
       61 taaataacct gatactgttg tgtgttttca aagaaaacac aggtattttt cacataaacc
      121 ttcccctttt catattcgac cacttaatca aaaaacggtt ttatacttgt tgcccccact
      181 ggggttaaat gtgagacatg tcgcactttg tgccatgtgt cctcgctgat tttaggcgcg
      241 gttcattcat tctttctgtt ggcataaagt atggtatcgg ttgggcgaat gaaagggact
      301 cgggtcatgg tcagacaata cctgaggcgt taattcgttg aaagagattg tgaatggggg
      361 aataactgag tcttaactgc tgtgcgatag cccggacaga tagttgctcg tcgaatctga
      421 gacgtaatga ttctggcaaa taagtgcgaa agccggtgat atctagtgtt gtttgagagt
      481 tcatcgttcg cgtccattaa tacagatgga cgcgattgtt gccagacagg ataattttca
      541 caagacatcg cagatagagt acttacgata attttttatt ttatacaaca gatgcccacg
      601 aaaagaatta ttttcaatag acacctgcat tacacaaag
//
LOCUS       DAJTNW010000296          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTNW010000296 DAJTNW010000000
VERSION     DAJTNW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.308"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            48..167
                     /locus_tag="OO179_004299"
                     /pseudo
     CDS             48..167
                     /locus_tag="OO179_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            334..606
                     /locus_tag="OO179_004300"
     CDS             334..606
                     /locus_tag="OO179_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534105.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          159 a          171 c          156 g          148 t
ORIGIN      
        1 cggttccaca aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga
       61 cctggatcac tgaagcttta cgccttcatt tcgaagaaca tttaccacgg gttgtggccg
      121 gacgtcgcct gggcgtacca aaatcaactg tctgtggtat gttcgtgtga accgcccccg
      181 gtatcccgct catgctggcg aacccagaca cgcagtgaac cgccccgggt ttcctggaga
      241 gtgttttatc tgtgaaccac ggcagcctgg cggctgcaaa tcggggtgtg gcagaatatg
      301 agctttcaga gtaaccatcc agaatgagtt cccatgtcac acaacctgga gcaccagaaa
      361 gtccacacca gaatggtgaa agaggttctg aaagccgtcg ccagagccaa taatcaccct
      421 tataagtccg tatttgcaga ctttatcacc ggtcatccct cctgcacggt ctgtttctgg
      481 gaaaccttcc acaaaatgta cccggattca ccgtacgaat acgtcacgtt ctgccatacc
      541 tgccgtcgtt ttgatttata cgaaacagaa gcggagatga aggcggatga cccgaagtgg
      601 tggtgatttt cagccccgac cgctgaaacg cctg
//
LOCUS       DAJTNW010000297          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTNW010000297 DAJTNW010000000
VERSION     DAJTNW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>626
                     /gene="virF"
                     /locus_tag="OO179_004301"
     CDS             <1..>626
                     /gene="virF"
                     /locus_tag="OO179_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5534106.1"
                     /translation="GHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQIAFI
                     ERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLNKKIF
                     LLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFSDQIR
                     KIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMH"
BASE COUNT          226 a           92 c          108 g          200 t
ORIGIN      
        1 ggacataaaa acaaaataga tataaaggtt cgcttgcata actatattat tttatatgca
       61 aaaaggtgtt caatgacggt tagctcaggc aatgaaactt tgactatcga tgaagggcaa
      121 attgctttta tagagcgaaa tatacaaata aacgtctcca taaaaaaatc tgatagcatt
      181 aatccatttg agattataag ccttgacaga aatttattat taagcattat tagaataatg
      241 gaaccaattt attcatttca acactcctat tctgaggaga aaagggggtt aaacaaaaaa
      301 atattcctcc tctctgagga ggaggtttct atcgatttgt tcaaatctat aaaagagatg
      361 cctttcggca aaagaaagat ctatagttta gcttgccttt tatcagctgt ttctgatgag
      421 gaagctttat atacttcgat atcgatagct tcttctctta gtttttctga tcagataagg
      481 aagattgttg aaaaaaacat cgagaagaga tggcgtcttt ctgatatttc aaataacttg
      541 aatttatcag aaatagctgt tagaaaacga ttggagagtg aaaaattaac atttcaacaa
      601 atccttcttg atattcgcat gcatca
//
LOCUS       DAJTNW010000298          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNW010000298 DAJTNW010000000
VERSION     DAJTNW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..479
                     /locus_tag="OO179_004302"
     CDS             <1..479
                     /locus_tag="OO179_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5534107.1"
                     /translation="AMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGY
                     WGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTI
                     EVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          179 a          129 c          139 g          176 t
ORIGIN      
        1 ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat tctgtacggc
       61 gtaaattcgg ggggcgctta cattcccggg aggatgggat aaaaccattt attcagtcag
      121 tacgccaggg atactggggg tattaccttc cagatcagga tcatggtcct gaatacagtg
      181 aatttgctga tttttttgcg acctataaag cgacattacc aattattgga cgtctgatga
      241 acatcagtca ggctatgatt ataccgcttt tcccggttta tgatgaaaaa aaacatttcc
      301 tgactattga agttcggcca ccaatggatg catgcattgc cagcgcggac aataaaatga
      361 ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gtcacatccg gaacagtata
      421 tctgggtttt aaaattgtta aaaacgcgca aatcaaacga agcggacccg tacccttgaa
      481 attataaagt acaggtattt ccactagttg tttcttacag gttaccaatc gaaacacatc
      541 ccccttccgt gtgttgcccg tttggcacgg aggactgtgt agatgcagtt cgttaaattt
      601 aatttatgcc taaatagctg cgc
//
LOCUS       DAJTNW010000299          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNW010000299 DAJTNW010000000
VERSION     DAJTNW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            2..508
                     /locus_tag="OO179_004303"
     CDS             2..508
                     /locus_tag="OO179_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5534108.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          167 a          134 c          147 g          157 t
ORIGIN      
        1 gattcctgtt attaccaccg aacttttggc gcagctttac ggcactgagc cggtgcgtat
       61 tcgccagaat catcatgaga acaaagtacg cttcgttgaa gggaaacact ttttcaaagt
      121 tgttggtaat gaccttaaag aattgcgggt cgctttaaac tactcacaaa atccagtttc
      181 acccaaagcc cgctccctca tcctctggac agaacgaggc gcagcacgcc acggcaaaat
      241 gctcgaaacc gatcaggcgt gggatgtgtt cgaaaaactg gaagactgct atttcagtca
      301 aagggatccg tcaacgccag tttcatgcca gaaaagttac gacacgcgag ttctctgtta
      361 tcagagaggc ggtgtcactg tttccacaat tcagttacgg gatgatgata ttgttatttc
      421 ccttgagtca tggctggaac tggcgagagc caatggttgg tttgttgttc gcagagataa
      481 actggtggaa aggttgatgc agctttaaaa aagttcttgc atttttgcac ataaactgct
      541 tcaattccgg taagcttcgc aaagctgtat cgcgaggcga atagcagaca tggacacttg
      601 aaaga
//
LOCUS       DAJTNW010000300          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTNW010000300 DAJTNW010000000
VERSION     DAJTNW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.312"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            264..>601
                     /locus_tag="OO179_004304"
     CDS             264..>601
                     /locus_tag="OO179_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5534109.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIP"
BASE COUNT          216 a          112 c           84 g          189 t
ORIGIN      
        1 gactaaaata tgatgtaaaa caaatatact tactcggatt acatttcctt gctcactgga
       61 atgttgaatt cagtgttatt attgactgca gtcacaataa aaaaagaatt ccatagaaaa
      121 cctccataaa taaattacaa ctaacttctg ttatgtgtaa aatggaaact attaaaactt
      181 aatatcggaa atggtaagtg aaatttgcat aaatatacaa ttttaaatat ttattctcac
      241 aaatataagg ttgacctagc attatgttct ctgtaaataa tacacactca tcagtttctt
      301 gctccccctc tattaactca aactcaacca gtaatgaaca ttatctgaga atcctgactg
      361 aatgggaaaa gaactcttct cccggggaag agcgaggcat tgcttttaac agactctccc
      421 agtgctttca gaatcaagaa gcagtattaa atttatcaga cctaaatttg acgtctcttc
      481 ccgaattacc aaagcatatt tctgctttga ttgtagaaaa taataaatta acatcattgc
      541 caaagctgcc tgcatttctt aaagaactta atgctgataa taacaggctt tctgtgatac
      601 c
//
LOCUS       DAJTNW010000301          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNW010000301 DAJTNW010000000
VERSION     DAJTNW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            306..>574
                     /locus_tag="OO179_004305"
     CDS             306..>574
                     /locus_tag="OO179_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5534110.1"
                     /translation="MVTVNFHCPRCQSVQVYRHGQNPKGHDRFRCRDCHRVFQLTYCY
                     EARKPGVKNQITEMAFNGAGVRDTGRTLKIGINTVIRTLKSSRPGG"
BASE COUNT          160 a          127 c          120 g          167 t
ORIGIN      
        1 acgccctgtg gttgaaacgt gagcatattg ttttcagatg ctgttgtctt gcttcagagt
       61 gatctgtcct ccggatttac gcaaagttat ccacaggcac tgtgcattta aaaaacttat
      121 cttttcttct tgattttaaa aactcagatc gtcatatcat tatgataaac ataataaaaa
      181 actctaaaca tgagaaggtt tttaacgtaa ggtgagagga tttttaagtg tatccataga
      241 ttaaaaatga caatgcctcc aattagttga attaggggtt ataatacccg cttttaggtt
      301 ctttcatggt cacagttaac tttcattgcc cccgttgcca gtccgttcag gtttaccgtc
      361 acgggcagaa cccaaaagga catgatcgat tccgttgccg tgactgtcac cgggtgtttc
      421 agctcactta ctgttacgag gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg
      481 ctttcaacgg tgccggagtt cgcgatactg gccgtacgct gaaaatcggc atcaataccg
      541 tcatacggac attaaaaagc tcccgccccg gcgg
//
LOCUS       DAJTNW010000302          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTNW010000302 DAJTNW010000000
VERSION     DAJTNW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.314"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            47..544
                     /locus_tag="OO179_004306"
                     /pseudo
     CDS             47..544
                     /locus_tag="OO179_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          136 c          175 g          115 t
ORIGIN      
        1 tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga cggcagaaga
       61 agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg gattaccaga
      121 gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt cgctgcatga
      181 atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg aagcattcag
      241 ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc tggcggaggc
      301 ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga aaaattacgt
      361 gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc tgatcggcac
      421 ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga gcgtactgcc
      481 ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc taccatcagg
      541 ataatcatca atacggtgtt cggtgaa
//
LOCUS       DAJTNW010000303          556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNW010000303 DAJTNW010000000
VERSION     DAJTNW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..297
                     /locus_tag="OO179_004307"
     CDS             <1..297
                     /locus_tag="OO179_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5534111.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>556)
                     /locus_tag="OO179_004308"
     CDS             complement(405..>556)
                     /locus_tag="OO179_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534112.1"
                     /translation="DPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKML
                     FMCRE"
BASE COUNT          182 a          131 c          102 g          141 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgt
//
LOCUS       DAJTNW010000304          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNW010000304 DAJTNW010000000
VERSION     DAJTNW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..187
                     /gene="glgS"
                     /locus_tag="OO179_004309"
     CDS             <1..187
                     /gene="glgS"
                     /locus_tag="OO179_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5534113.1"
                     /translation="NSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTWFCARY
                     AWYCQQMMQARELELEH"
     gene            complement(230..>545)
                     /locus_tag="OO179_004310"
     CDS             complement(230..>545)
                     /locus_tag="OO179_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5534114.1"
                     /translation="FSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLK
                     IFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKIT
                     YN"
BASE COUNT          151 a          112 c          126 g          156 t
ORIGIN      
        1 taattctttg aataatttcg atttcctggc gcgtagtttt gccagaatgc acgcagaagg
       61 tcgcccggtc gatattctgg ctgttactgg taacatggat gaagaacata gaacctggtt
      121 ttgcgcacgt tatgcctggt attgccaaca gatgatgcag gcaagagagc tggagttaga
      181 gcactgatat aacgggcctg atggcccgtt ttagtgtttg tattagatgc tagttatagg
      241 tgattttgag gattacgtct ttactaaacg gtccctcaac aacagtctct gccgggttcg
      301 cactaagttc tgccctgaat ggtgcggtga ccgaaggtgc actagacgat gaaaaagaag
      361 caaattgtga gaagcggtta aattccgttg ctgttccatc tttatgaaat atcttcaata
      421 gcagtccatt acccatatcc agatggctat aatcactcaa aatatccgta gtgaaaaagc
      481 tccctaaaat attgaattgc tcagtacagg ccgcaccagg atcttttgtc atgactacac
      541 taaac
//
LOCUS       DAJTNW010000305          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTNW010000305 DAJTNW010000000
VERSION     DAJTNW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.317"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>539)
                     /locus_tag="OO179_004311"
     rRNA            complement(<1..>539)
                     /locus_tag="OO179_004311"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          112 a          162 c          130 g          135 t
ORIGIN      
        1 cccgaaggtt aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt
       61 gtacaaggcc cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg
      121 acttcatgga gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc
      181 ttgctctcgc gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt
      241 cgtaagggcc atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc
      301 tcctttgagt tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg
      361 acttaaccca acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt
      421 tcccgaaggc acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc
      481 ttcgcgttgc atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTNW010000306          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTNW010000306 DAJTNW010000000
VERSION     DAJTNW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.318"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..212
                     /locus_tag="OO179_004312"
                     /pseudo
     CDS             1..212
                     /locus_tag="OO179_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            355..>539
                     /locus_tag="OO179_004313"
     CDS             355..>539
                     /locus_tag="OO179_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5534115.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIHQMAEYNRLLSQRMAAYASEI
                     NRLKALVAKLQRMQFGK"
BASE COUNT          146 a          140 c          141 g          112 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatgggggcg
      121 gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag ttacacagtt
      181 gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc gggaaaccgg
      241 tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc
      301 caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga ggaaatggac
      361 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact
      421 atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc
      481 gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cggtaaaag
//
LOCUS       DAJTNW010000307          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTNW010000307 DAJTNW010000000
VERSION     DAJTNW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.319"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>539
                     /locus_tag="OO179_004314"
     CDS             <1..>539
                     /locus_tag="OO179_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5534116.1"
                     /translation="SYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHG
                     IASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQ
                     EKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKES
                     IVNWNALYSSDDVLHKIGPG"
BASE COUNT          211 a           77 c          108 g          143 t
ORIGIN      
        1 tcatacggca tgatgattta taaaaatgat aaaacatttc gtaatcttga aatattcgga
       61 gacagcggct ctggagcata cttatatgat aacaaactag aaaaatgggt attagtcgga
      121 acaacccatg gtattgccag cgttaatggt gaccaactga catggataac aaaatacaat
      181 gataaactgg ttagtgagtt aaaagatacc tatagtcata aaataaatct gaatggcaat
      241 aatgtaacca ttaaaaacac agatataaca ttacaccaaa acaatgcaga taccactggt
      301 actcaagaaa aaataactaa agacaaagat attgtgttca caaatggggg aaatgttctg
      361 tttaaggata atttggattt tggtagcggt ggtattatct ttgacgaagg ccatgaatat
      421 aacataaacg gtcagggatt tacatttaaa ggagcaggaa ttgatatcgg aaaagaaagc
      481 attgtaaact ggaatgcatt gtattccagt gatgatgttt tacacaaaat aggccccgg
//
LOCUS       DAJTNW010000308          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTNW010000308 DAJTNW010000000
VERSION     DAJTNW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.320"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            19..>538
                     /locus_tag="OO179_004315"
     CDS             19..>538
                     /locus_tag="OO179_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5534117.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELA"
BASE COUNT          148 a          107 c          160 g          123 t
ORIGIN      
        1 cacagagctg gagtgtgcat gaataaccag tatttacagt ttgttcgtga gcagctcatt
       61 atcgccaccg ctgatttgag tggggcgaca aaaggtcagc ttgaagcctg gcaggagaat
      121 gccatgttcg atacagggcg ttacaggcgt aaaaaaatcc ggtaccgcga tgaggtgacc
      181 ggaaaaatga taacgcggga taatccacca atcccgggaa aacaatcact ggcgaagggg
      241 gcgtcaattc ctctggtcag tcaggttgcg ttttcgacat catcgtggcg gcgggctgtt
      301 ctgtctcttg aagagcacta taaagcctgg ttgttgtggt gttacagcgg aagtatttgc
      361 tgggagtatc agatcaccat aacccactgg gcgtgggaag agtttaaggc tcattctggc
      421 actaggaaaa ttgcagagaa aacacaggaa cgcctgaaaa aattaatctg gctggcggcg
      481 caggcggtaa aagcagaact ttttggtggg gaaggttatg aatatcagga gctggcat
//
LOCUS       DAJTNW010000309          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTNW010000309 DAJTNW010000000
VERSION     DAJTNW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.321"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>516
                     /locus_tag="OO179_004316"
     CDS             <1..>516
                     /locus_tag="OO179_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5534118.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLT"
BASE COUNT          113 a          163 c          139 g          101 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgtt
//
LOCUS       DAJTNW010000310          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTNW010000310 DAJTNW010000000
VERSION     DAJTNW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.322"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            90..155
                     /locus_tag="OO179_004317"
                     /pseudo
     CDS             90..155
                     /locus_tag="OO179_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            383..>516
                     /locus_tag="OO179_004318"
     CDS             383..>516
                     /locus_tag="OO179_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5534119.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     T"
BASE COUNT          166 a          105 c          119 g          126 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccac
//
LOCUS       DAJTNW010000311          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTNW010000311 DAJTNW010000000
VERSION     DAJTNW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.323"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            29..>514
                     /locus_tag="OO179_004319"
     CDS             29..>514
                     /locus_tag="OO179_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5534120.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WK"
BASE COUNT          129 a          122 c          166 g           97 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaag
//
LOCUS       DAJTNW010000312          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTNW010000312 DAJTNW010000000
VERSION     DAJTNW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.324"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..269)
                     /gene="ttdR"
                     /locus_tag="OO179_004320"
                     /gene_synonym="dan"
     CDS             complement(<1..269)
                     /gene="ttdR"
                     /locus_tag="OO179_004320"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5534121.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQ"
BASE COUNT          128 a          115 c          119 g          141 t
ORIGIN      
        1 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
       61 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
      121 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
      181 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
      241 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
      301 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
      361 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
      421 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
      481 gagcgaaagt aataagcaac agg
//
LOCUS       DAJTNW010000313          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTNW010000313 DAJTNW010000000
VERSION     DAJTNW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.325"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..503
                     /locus_tag="OO179_004321"
                     /pseudo
     CDS             1..503
                     /locus_tag="OO179_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          127 a          121 c          168 g           87 t
ORIGIN      
        1 cggatgttca tcagttacag cgaagtcagc ataaaaaatc ccgacaacag cggccaggcg
       61 ctcccgctca cgtatgtctg ttgccgggag caggcagagg acggtgcctg ctggcatctg
      121 ctgacgtcag gaaaagcggc gagtgccgcc gatgcacgac gtattgtcag ccattacgag
      181 cgacgctggc tgacagagga ataccacaag gcgtggaaaa gtggtggtac atggaatcgc
      241 tgcgaatgca gacccgggat aaccttgagc gcatggtggt tatccaggcg tttatagcgg
      301 tacgggtgct gggcctgcgg cagggaggtg tcagtgaaga aacacaaaat gacagctgtg
      361 agaaaatact gacgccgaca gagtggaaac tactgtgggt taagctggaa ggaaagccgc
      421 ttccggtgca ggcaccgacc ctgaaatggg cctggggcga tggcatgaca gcaaacgcac
      481 aggtcgtccc ggttggggcg tca
//
LOCUS       DAJTNW010000314          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTNW010000314 DAJTNW010000000
VERSION     DAJTNW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.326"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>495)
                     /locus_tag="OO179_004322"
     rRNA            complement(<1..>495)
                     /locus_tag="OO179_004322"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DAJTNW010000315          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTNW010000315 DAJTNW010000000
VERSION     DAJTNW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.327"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..490
                     /locus_tag="OO179_004323"
                     /pseudo
     CDS             1..490
                     /locus_tag="OO179_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          136 a          125 c          126 g          103 t
ORIGIN      
        1 aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccggaag cgggctgctt
       61 ttccccgatt caaaaagcgg tgacagaatg atgcattccg ctacccgcag ggcgttaagc
      121 tcgatcagga aaacagccgt atttttctgc cgaaactggg ctggatgcgc taccggaata
      181 gccgtcaggt cacgggtgtt gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt
      241 acatcagtat tcagacagaa agtgaagtat ccactccggc tcacccttca gcatcaatgg
      301 tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca gtctttgagc
      361 ctgtaaacag ttttcagaaa aaccagaaga cgctggcgag acttcagcga cagttaagcc
      421 gcaaggtcaa attcagcaac aactggcaga agcagaaacg caaaatacag cgactgcatt
      481 cctgtatcgc
//
LOCUS       DAJTNW010000316          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTNW010000316 DAJTNW010000000
VERSION     DAJTNW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.328"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(144..374)
                     /locus_tag="OO179_004324"
                     /pseudo
     CDS             complement(144..374)
                     /locus_tag="OO179_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          146 a          122 c           90 g          109 t
ORIGIN      
        1 ttgtaggcca gataagacgt tcacgtcgca tccggcatga acaaagcgca ctttgtcaaa
       61 aatctaacct actttttaat tcagggaatt accgcaaagc ccacgccatc atacccaacg
      121 taacaaaact caggcacgtt ccccactcgc cccgagaaaa atagcattaa tgcgcccagc
      181 gtcagcataa aaatattgag cagaaggtaa attgttggcg taaagagtaa attgcaaacc
      241 tcaggaatag cggcaagccc agcccccagc atggcaataa ccgctgcaaa aaattgcgtc
      301 agtattggga tgccgaaaaa agtcattacc agcgaggtca aaatccattt tctattttgc
      361 attattcttt ccattctttc tgaatggcga aattatactc cccgagtccc cttgcccctt
      421 ctggacactt ttccgaaatg atggcgaaaa aaacgggacc ctttggc
//
LOCUS       DAJTNW010000317          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTNW010000317 DAJTNW010000000
VERSION     DAJTNW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.329"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(242..>465)
                     /locus_tag="OO179_004325"
     CDS             complement(242..>465)
                     /locus_tag="OO179_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5534122.1"
                     /translation="CELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKANSGDTM
                     LDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          138 a          103 c           78 g          146 t
ORIGIN      
        1 cgacataact gcgtgcaatg gattttgaag gaaccgtcag cccattcccc ggaggcaaac
       61 atgggctggc aaaagtgtag cggtattagg cacagctatt taggaaagag ggaccatctc
      121 catctcatta aatggaccac gccagacaat tcactgtcat gctgatgctg cactctgggg
      181 acagaatcac tcatgatgac ttcgataatc gacgacatta ttatttggcc gagcttttag
      241 gtcatatctc aaatcgtttg cctaatattg ctccattttt taataaaaaa tcaatcattt
      301 ttgaatcttt gcttttcatt gcattgtcaa gcatagtgtc cccactattt gctttttgaa
      361 atcttttgtt tacatcaacc aatccattac taataaaata ttctagcact ttatagttgg
      421 cactatcttc actaagtaaa tactcaacat catacagctc acaat
//
LOCUS       DAJTNW010000318          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTNW010000318 DAJTNW010000000
VERSION     DAJTNW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.330"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          133 a          129 c          105 g           97 t
ORIGIN      
        1 ccgcaagcgg cgggccccaa ccgaagccga tttagttaca acatgcaaac ataaacccct
       61 aaaaaaccca agtccaacac agaactgaaa ccacaacgcc cccctcataa ctgaaaagcg
      121 ccgccgcccc cgcccaaagg gcgggaacag agtcgctatt aattatgaat gttgtaacta
      181 catcatcatc gctgtcagtc ttctggctgg acgtatcgag tacacgctcg taagcggccc
      241 tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg tcgggaccac
      301 tccgaccgcg cacaaaagca ttttcatcgc cgatagcggg tatggcttgg cagggccggg
      361 atgggtatag gtaatttggc tatcgtaaaa aaggtggtta aaaattacaa cctgcaatgt
      421 aaattggcta gttaattttt ggctatttaa attgacttct taac
//
LOCUS       DAJTNW010000319          455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTNW010000319 DAJTNW010000000
VERSION     DAJTNW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.331"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>455)
                     /locus_tag="OO179_004326"
     CDS             complement(<1..>455)
                     /locus_tag="OO179_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5534123.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          108 a          125 c          124 g           98 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg cccctgtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacc
//
LOCUS       DAJTNW010000320          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTNW010000320 DAJTNW010000000
VERSION     DAJTNW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.332"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            154..>454
                     /locus_tag="OO179_004327"
     CDS             154..>454
                     /locus_tag="OO179_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5534124.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAG"
BASE COUNT          122 a           94 c          130 g          108 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcc
//
LOCUS       DAJTNW010000321          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTNW010000321 DAJTNW010000000
VERSION     DAJTNW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.333"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>452
                     /locus_tag="OO179_004328"
     CDS             <1..>452
                     /locus_tag="OO179_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534125.1"
                     /translation="STGFTQGTLQQDAMNICENVVRTINARMVDVLESSKLLKTVELP
                     VLTGSLTAKADAIMDALYENTEPSFGSEVSDYGIIAHESQLKALSRLAAKQGFSGEDA
                     IVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITVVLSRDG"
BASE COUNT           98 a          119 c          122 g          113 t
ORIGIN      
        1 tccaccggat tcactcaggg cacacttcag caggacgcta tgaacatctg tgagaatgtg
       61 gtccgtacca tcaacgctcg catggtcgac gttctggaat cctctaagct gctgaagacc
      121 gtagagcttc ctgtcctgac tggtagcctg accgctaagg cagacgctat catggacgct
      181 ctgtatgaga acaccgagcc ttctttcggt tctgaggtgt ctgactacgg tattatcgcc
      241 catgagagcc aactgaaagc actgtcccgt ctggctgcta aacagggctt ctctggtgaa
      301 gacgctatcg tggatatgct gggtaccgac atcgcgtatt acaacggtga ggacaaaggt
      361 gtgttcatgt tggctaaacg cttcacggcc ctgagcttcg gctgcttccg tcacgacggt
      421 gagaatatta cggtcgtcct ttcacgtgat gg
//
LOCUS       DAJTNW010000322          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTNW010000322 DAJTNW010000000
VERSION     DAJTNW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.334"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(15..230)
                     /locus_tag="OO179_004329"
     CDS             complement(15..230)
                     /locus_tag="OO179_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5534126.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          145 a          107 c           95 g          101 t
ORIGIN      
        1 taatgatggc gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag
       61 tacagatttg tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca
      121 gcccactgtg acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct
      181 gcggaggtgc cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct
      241 ccgcaaataa cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa
      301 accgcaaaag cgggcgaaac gatatataca gtaaggaaag cactctatcc aacaaaccac
      361 tcacagttaa tcggaataaa agcagagtgc ttatgaatga tcgcctgccc gaaggttagt
      421 atttctgcac agcaattttg caaaaaaa
//
LOCUS       DAJTNW010000323          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTNW010000323 DAJTNW010000000
VERSION     DAJTNW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.335"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(41..193)
                     /locus_tag="OO179_004330"
                     /pseudo
     CDS             complement(41..193)
                     /locus_tag="OO179_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            249..>447
                     /gene="ospZ"
                     /locus_tag="OO179_004331"
     CDS             249..>447
                     /gene="ospZ"
                     /locus_tag="OO179_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5534127.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEK"
BASE COUNT          151 a           79 c           86 g          131 t
ORIGIN      
        1 tgaggctctg tgagcctacc cacgtaatat ggacacaggc ctaagcgagg ttctggtttt
       61 caaattgttc cggactgagg ccgccacacc aacggtgccg tcgccagcga ttgtaattgc
      121 actcgatata attaaacact gttgcccgca ctatttcccg atagataaag tgttctccgt
      181 ggagacattc cacttaggaa caattatatt gttcatggta atgtttatat ttgagtatag
      241 agattaaaat gattagtccc atcaagaata ttaagaacgt atttccaata aatactgcaa
      301 atactgaata tatagtgaga aatatttatc ctagagttga acatggttac tttaatgaat
      361 caccaaacat atatgacaag aagtatatct ctggtataac cagaagtatg gcacaattaa
      421 aaatagagga gtttattaat gagaaat
//
LOCUS       DAJTNW010000324          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTNW010000324 DAJTNW010000000
VERSION     DAJTNW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.336"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..309
                     /locus_tag="OO179_004332"
     CDS             <1..309
                     /locus_tag="OO179_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5534128.1"
                     /translation="IIVSSAGNQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGN
                     KDRMVAYTNDKKYVRYPLVPLQSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI
                     "
BASE COUNT          108 a          115 c          112 g          100 t
ORIGIN      
        1 atcatcgttt catctgcagg taaccagtcc ctgttgacga accttcagac caacaccatc
       61 agctatcacc agaacggtgt tccgctgaat atccgcgcgg ttaaatggct gaaaggccgt
      121 ggtgtgggga ataaggatcg catggttgcg tacaccaacg ataaaaaata cgtccgctac
      181 ccgctggttc cgcttcagag cgtgccggtg cagtatcgcg gtctgtatca gatcgtcact
      241 tactacggca agctgggtgc agtcgagcca gtgtacaaag aaaccatttc gtacgttgat
      301 ggcatttaac agccacatgg ccccctggcg gggccattaa ggatgacccg atggcaaaaa
      361 atgatgcagt aatacacgta cataccccgt ttgtgctcac gcttcccgac ggttctcggc
      421 gcgagtttgt taaag
//
LOCUS       DAJTNW010000325          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTNW010000325 DAJTNW010000000
VERSION     DAJTNW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.337"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          123 a           84 c           82 g          144 t
ORIGIN      
        1 cataatgtca cttaccctca tcggtaaaaa aattgccttt cgaccattac gatacattat
       61 tgattttggg gtttcaggga agtaatccat ttcgactata actgacaggt catcacgatg
      121 tatgactgcg tatttgctac taaatagttt ctttattttt tccacgatgc ccccaggttt
      181 ataagtacaa acggttatat ccacatagag acaaaaatat taatctgata aatatttatt
      241 tcacgccgta tatttgattg tttaatgtgc aagtacaatg acttttattt tttgttgtgt
      301 atataatcaa atatatggtt atttttcacc ctgcgtattc agcacgcaac aaaaaacccg
      361 ccgaagcggg tttagtgcgg gtgcgttgag gatgcctgac acatgagagg tggcgaggga
      421 tttctccccc gcc
//
LOCUS       DAJTNW010000326          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTNW010000326 DAJTNW010000000
VERSION     DAJTNW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.338"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>429)
                     /gene="queE"
                     /locus_tag="OO179_004333"
     CDS             complement(<1..>429)
                     /gene="queE"
                     /locus_tag="OO179_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5534129.1"
                     /translation="GVPAIFIRLQGCPVGCAWCDTKHTWEKLEDREVSLFSILAKTKE
                     SDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSG
                     THEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKHPV"
BASE COUNT           95 a          128 c          104 g          102 t
ORIGIN      
        1 actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacttc atagccgccg
       61 cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg tacttcatga
      121 gttccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcaggtc agtcagtggc
      181 agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc ggtatatccc
      241 tgacgaccaa tgacagccag caagtcttca ctgctcgccg ccccccactt atcactctct
      301 ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt ttcccaggtg
      361 tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat aaaaatggcg
      421 gggacgccg
//
LOCUS       DAJTNW010000327          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTNW010000327 DAJTNW010000000
VERSION     DAJTNW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.339"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..406)
                     /locus_tag="OO179_004334"
     CDS             complement(<1..406)
                     /locus_tag="OO179_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR5534130.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQ"
BASE COUNT          117 a          106 c           93 g          113 t
ORIGIN      
        1 actggtattc cgtcagtgcg tgggcttctt ccagccacaa catatcgatc ccctccagtg
       61 acttaatttc ttcgatgttg cgccataatc cataaaaaac aaattccgag ccagtaaccc
      121 ggttaatgat tttgttgttc agaatacgga aacggtgccg cagaccaaac ctgtctatct
      181 gaattttgag cagggtatac accgactctt cgattttatt ctggatctga cgggcacaac
      241 aaaaacgcag ggtgtattta ttcgacagaa atatggcaat gccagcggca tcccatgatt
      301 ttgacgatga ccgaccacca taaagcactt tgttacgtgc ccgcatagtc cagaaactac
      361 gtaaagccgg attcagcgtc ggtttggatg tcagagtaga agtcattgag gtcacgctct
      421 ccattgcca
//
LOCUS       DAJTNW010000328          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTNW010000328 DAJTNW010000000
VERSION     DAJTNW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.340"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>428
                     /locus_tag="OO179_004335"
     CDS             <1..>428
                     /locus_tag="OO179_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5534131.1"
                     /translation="ELERLDQTLNLPLVGYTWSRDIQLREDASIADDISSWTNTSFAA
                     AGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGMELGWTVIELQAAQQVGRPI
                     DTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV"
BASE COUNT          110 a          105 c          128 g           85 t
ORIGIN      
        1 gagctggaga gactcgacca gacgctgaac ctgccactgg tggggtacac ctggagccgc
       61 gatattcaac tgcgtgaaga cgcctccatc gcagatgaca tttccagctg gacgaatacc
      121 agcttcgccg ctgcgggtac tggcgcaaat ccgaatggaa aaaactgggt aggcaaagac
      181 tcaaccgcta ttgctggcgt gaacgtggat atcggcaaat ccggtaaccc gctgaacctg
      241 tgggggatgg aacttggctg gacggtcata gaattgcagg ctgctcagca ggtcggccgc
      301 ccgattgata cgcagaagta tgacggtatg caactgaaat ggcagatgga taacgatgaa
      361 caggtatatg ttggcgattc cgcattaaac ctgaaaggcc ttgttaccct ggacggcgtg
      421 cctgtcaa
//
LOCUS       DAJTNW010000329          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTNW010000329 DAJTNW010000000
VERSION     DAJTNW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.341"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>424
                     /locus_tag="OO179_004336"
     CDS             <1..>424
                     /locus_tag="OO179_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5534132.1"
                     /translation="RDAGTSASEAATNATDAAGSARAASTSAGSAASSASSASASKDE
                     ATKQASAAKGSATTASSKATESAGSATAAAQSKSAAESAAMRAETAAKRAEDIADVVS
                     LEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDET"
BASE COUNT          112 a          113 c          140 g           59 t
ORIGIN      
        1 ccagggatgc cggcacatct gccagtgagg cggcaaccaa tgcgactgat gctgcaggct
       61 cagcacgcgc agccagcacg tcagccggtt cggcagcgtc atcggcatca tctgcgtctg
      121 cttcaaaaga tgaggcgact aaacaggcgt cagcagcgaa gggcagtgcc acgacggcat
      181 cctctaaggc gacagagtct gcaggcagtg cgacggcggc agcgcagagc aaaagcgcgg
      241 cagaatccgc ggcaatgcgc gctgagacag cagcaaaacg ggcagaggat attgctgatg
      301 tcgtatctct ggaagatgcg agcacgacga aaaaggggat agtgcagctc agcagtgcga
      361 ccaacagtgc atccgagtca ctggccgcga cagcgaaagc ggttaaggtg gtaatggatg
      421 aaac
//
LOCUS       DAJTNW010000330          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTNW010000330 DAJTNW010000000
VERSION     DAJTNW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.342"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..247)
                     /locus_tag="OO179_004337"
     CDS             complement(<1..247)
                     /locus_tag="OO179_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5534133.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGCYTARTLKIGINTVIRT"
     gene            170..>416
                     /locus_tag="OO179_004338"
     CDS             170..>416
                     /locus_tag="OO179_004338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534134.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          120 a           89 c          101 g          106 t
ORIGIN      
        1 atgtccgtat gacggtattg atgccgattt tcagtgttct ggcggtataa cagccccctg
       61 cgccgttatg tgccatttca acgatctgct ccttaacgcc gagttttctg gcttcataag
      121 agtaagtgag ctggaaaaca cgtttacatg agcgacaacg gaaacgctca tgccgggaac
      181 aactacggcc atgtcgataa acttcatcgg aattacagcg cggacaatga acagtaacgg
      241 tagtcatgga aaacctcaaa taccagtaga ttaccagaaa cttctatcga ttgaagacat
      301 taccttttgc cgcaataggt ggggtaacat tggtgaaaac gcattacgcc gggtggcagt
      361 tggaaaaaaa ttatcttttt tcggctctga tcgtggtgga gaaaatgcgg caataa
//
LOCUS       DAJTNW010000331          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTNW010000331 DAJTNW010000000
VERSION     DAJTNW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.343"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(1..416)
                     /locus_tag="OO179_004339"
                     /pseudo
     CDS             complement(1..416)
                     /locus_tag="OO179_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          114 c           96 g          106 t
ORIGIN      
        1 gggcgtgtac tgagagacca gctcacaact tttccatcga agcagtccac cactggtgac
       61 agccagactt tacctgcagg gagctggaac tccgtgatat ctgtcagcta tttctgatta
      121 ggttgctccg ccttaaaatc tctggcgata aggttatccg gagccggtcc gatttctccg
      181 caatatgagc tgtagcgacg acgacggtta cgactgacga caagctgttc ctccaccata
      241 agtctgcgta caaccttttc tgatagccgc cccccctcat gcctgagcat cgcatgcagg
      301 cgacgatagc cataacactg gtaattactg ttaaagatat ctgtcagcat cttacgtatc
      361 gtggcgtatt tatcaccggc tttcagtgca gcccggtgat aaaaataact gctacg
//
LOCUS       DAJTNW010000332          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTNW010000332 DAJTNW010000000
VERSION     DAJTNW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.344"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..412
                     /locus_tag="OO179_004340"
                     /pseudo
     CDS             1..412
                     /locus_tag="OO179_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          105 a          106 c          120 g           81 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cg
//
LOCUS       DAJTNW010000333          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTNW010000333 DAJTNW010000000
VERSION     DAJTNW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.345"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>409
                     /locus_tag="OO179_004341"
     CDS             <1..>409
                     /locus_tag="OO179_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5534135.1"
                     /translation="VTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGL"
BASE COUNT          117 a          102 c          111 g           79 t
ORIGIN      
        1 ggtcacgccg ggacagaatg aaaaatatta tctggccgga gcgctgcaca gcgggacagg
       61 taaagtcagc tatgtgggcg gcaacagcaa aagttcggcg ctgttcatca gcctgctgaa
      121 gcggcttaaa gcgacatacc gtcgtgcgaa aaccatcacg ctgatcgtgg acaactacat
      181 tatccacaaa agccgggaaa cacagagctg gctgaaggag aacccgaagt tcaggggcat
      241 ttatcagccg gtttactcgc catgggtgaa tcatgttgaa cggctatggc aggcacttca
      301 cgacacaata acgcgtaatc atcagtgccg ctcaatgtgg caactgttga aaaaagttcg
      361 ccattttatg gaaaccgtca gcccattccc cggaggcaaa catgggctg
//
LOCUS       DAJTNW010000334          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTNW010000334 DAJTNW010000000
VERSION     DAJTNW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.346"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..406
                     /locus_tag="OO179_004342"
                     /pseudo
     CDS             1..406
                     /locus_tag="OO179_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a          111 c          119 g           76 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaa
//
LOCUS       DAJTNW010000335          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTNW010000335 DAJTNW010000000
VERSION     DAJTNW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.347"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>402)
                     /locus_tag="OO179_004343"
     CDS             complement(<1..>402)
                     /locus_tag="OO179_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5534136.1"
                     /translation="LLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQV
                     QHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPV
                     VREYFFDRNQISHIPESILNLRNECSIHISD"
BASE COUNT          115 a           66 c           93 g          128 t
ORIGIN      
        1 ttatcactaa tatgtattga acattcattc ctcagattaa gaatgctttc cgggatatga
       61 cttatctgat ttctatcaaa aaaatactct ctgacaacgg gattatttcc ctcagaaaat
      121 gctggtagct cagtgaggaa attacgggtc gctctgagat ttttaagagc ctgtgggagc
      181 gatggtaagg agactatttc gttatagctg atattcatag tcaataacga atcgggtaaa
      241 gctggcaggt tttccagctc attgtgttgt acctgaagtg actccagcgc aggaggtagc
      301 acgggcaatt ctgataattt attgctggcg gaatataatt tttttagcgt aacaggcagt
      361 tcaggtaggt tagttaattg attatatgat acattgagca gc
//
LOCUS       DAJTNW010000336          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTNW010000336 DAJTNW010000000
VERSION     DAJTNW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.348"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>397
                     /locus_tag="OO179_004344"
     CDS             <1..>397
                     /locus_tag="OO179_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5534137.1"
                     /translation="FIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVD"
BASE COUNT          125 a           81 c           91 g          100 t
ORIGIN      
        1 atttattcgt tttattgagg cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc
       61 tgtatacacg ggtattcgtc acggggagct ggtatcactg gcctgggaag atatagacct
      121 taaagcgagg actataacca tccgccggaa ttatacaaaa ctaggcgaat tcactccacc
      181 aaaaactgat gctggtaccg gaagaacgat tcatctagtt caaccagcta ttgatgctct
      241 taaaagccaa gcggaaatga ccatgcttgg aaaacagcat tctgtagagg taaagcagag
      301 ggaatatggg agaagtactg tgcataaatg cacttttgtt tttagtcctc aggtaataaa
      361 acagcggcag ttttccggac cgcactataa ggttgac
//
LOCUS       DAJTNW010000337          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTNW010000337 DAJTNW010000000
VERSION     DAJTNW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.349"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..216)
                     /locus_tag="OO179_004345"
     CDS             complement(<1..216)
                     /locus_tag="OO179_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5534138.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERY"
BASE COUNT          114 a           84 c           83 g          114 t
ORIGIN      
        1 ataacgttcg ttatccagca caaataaacg ttgatccagg ataatgtttt tagcttcctg
       61 gtacgccagt tccatgatga aatcggtacg gttcttattg accaagctcg cagcataatc
      121 aataacggct cgttcctcat cagttgcccg gatgttaatc tgatgttctc gcggtgtttt
      181 acggacgctt gcagctgtag acataaaaac ctccattggt aacaatttgc tatgcaatta
      241 atgttatcgc aaatggtaac agaacgctat acagaatcac ttacgggaaa agcgatcccc
      301 tgataaaaac aatggcagag acctgaacat tgtgacgatg atctgtgtcg gcgcacaaat
      361 catgtctgat tacggccgtt taatgtgatt ttgat
//
LOCUS       DAJTNW010000338          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTNW010000338 DAJTNW010000000
VERSION     DAJTNW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.352"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>382
                     /locus_tag="OO179_004346"
     CDS             <1..>382
                     /locus_tag="OO179_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5534139.1"
                     /translation="LPDNLPPQITVLEITQNALISLPELPASLEYLDACDNRLSTLPE
                     LPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFL
                     PELPESLEALDVSTNLLESLPAVPV"
BASE COUNT          115 a          104 c           61 g          102 t
ORIGIN      
        1 ctacctgaca acttaccacc tcaaatcact gttctggaaa ttactcagaa tgccctaata
       61 tcattaccag aattgccagc atcgctggaa taccttgacg cctgtgacaa tcgcctgtca
      121 acacttcctg aattacccgc atctctgaaa catcttgatg tagataacaa ccaactaacc
      181 atgcttcctg aattgcctgc attgctggaa tatattaatg cagataacaa tcagctaacc
      241 atgcttcctg aattacctac atcgctggaa gtgctctcag taagaaataa ccagctgaca
      301 tttctccctg agttacctga atcactggaa gcgctcgatg taagtactaa tcttctggaa
      361 agcctaccag ccgtacctgt aa
//
LOCUS       DAJTNW010000339          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTNW010000339 DAJTNW010000000
VERSION     DAJTNW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.353"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT           95 a           92 c          112 g           80 t
ORIGIN      
        1 aaatcctcgg taaagtgttc gttgtcgctg aagctgctac gaccgtcaag atgggcactg
       61 gctcagctac tgccgtcctg cctgtagtca aacgcctgaa gttcaccaag accgaagcgt
      121 aagaccaatg ggtaagagca ttagtaaagg cttcaggtcc atcgccaagg gcctgacagg
      181 tggtggtggg actcaaggtg gggcttcggt cccgcctcct gtagctcctc cagatgacgc
      241 tcagcaagct gtagtcgata cccaaggtca ggagaccgag ggaggtaaga ccatgagcag
      301 aggtaagtct ggactgaata tcagcaggac ttcaggtggt gggctatcac tgtgattaac
      361 taactatggt ctggaccaa
//
LOCUS       DAJTNW010000340          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTNW010000340 DAJTNW010000000
VERSION     DAJTNW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.355"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(178..>377)
                     /locus_tag="OO179_004347"
     CDS             complement(178..>377)
                     /locus_tag="OO179_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5534140.1"
                     /translation="IITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKR
                     GYRLAEQVLMHEPLARMRWCG"
BASE COUNT           91 a          119 c           74 g           93 t
ORIGIN      
        1 ttaaaacccc aaaaagatta aaaaaacacc acaaaacgga tgtttcttca acaccacttt
       61 tgctccatat gaacggaacc gacgattaaa ctggatggct ctgattgatt cagggtatga
      121 atggcggttt tttgctccgt ttccctcaaa atggacgcaa cttcccctct gcggctctca
      181 gccgcaccac cgcatccggg ccagcggctc atgcatcagg acctgctctg ccagacggta
      241 gccccgcttc agccccgtaa aacgcatctg actcccgcac agcacgcact tcagcgggtc
      301 aaccttcagt aacctctgat acatccctct ccaggtgatt tgcatcgccg tttttctcac
      361 tgtctccgtt atgatgt
//
LOCUS       DAJTNW010000341          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTNW010000341 DAJTNW010000000
VERSION     DAJTNW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.356"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            260..318
                     /locus_tag="OO179_004348"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO179_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTNW010000342          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTNW010000342 DAJTNW010000000
VERSION     DAJTNW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.357"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(111..>373)
                     /locus_tag="OO179_004349"
     CDS             complement(111..>373)
                     /locus_tag="OO179_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5534141.1"
                     /translation="TSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMG
                     EQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          104 a           96 c           76 g           97 t
ORIGIN      
        1 ttattttatc tcctacaaga ttatttaatg gaatgcaaaa tatctggcct gataacaatg
       61 ctccagaatt aatacctgca ggctatgtta aagttgcctc cacacccaat ctacctgttc
      121 tctcgtgtgt gccacaggcg caatacataa atagtctggc cagtaagttc gtaacggatt
      181 tcgtactcgc cagtaaaaat ccgtctgacc tccctgggtt caaactgaaa caactgttct
      241 cccatacgtg gatgagttaa cagaatgacc ggcgcctgtg tcagggactg caccgttctg
      301 gcggcagcag gtttactggc cagcaccaga aaatcatata accgcgccaa atcagaaagt
      361 gccttactgg tcc
//
LOCUS       DAJTNW010000343          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTNW010000343 DAJTNW010000000
VERSION     DAJTNW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.358"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..318
                     /locus_tag="OO179_004350"
     CDS             <1..318
                     /locus_tag="OO179_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534142.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          101 a           70 c          101 g           98 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc
//
LOCUS       DAJTNW010000344          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTNW010000344 DAJTNW010000000
VERSION     DAJTNW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.359"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..>363)
                     /locus_tag="OO179_004351"
     CDS             complement(<1..>363)
                     /locus_tag="OO179_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5534143.1"
                     /translation="DIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPA"
BASE COUNT           88 a          108 c           99 g           68 t
ORIGIN      
        1 cgccgggatg ccttcgtccg ggcgaccggt acagatgaac tcatacggcc ccagtttggc
       61 gatctcatcc gccagatagg cagcgacctg gtaggaagca ttctgtactt tggtatagcc
      121 ttcacgacca aggcgcagga attcatagta ctgtgcaatc acctgacccg ccgggcggga
      181 gaagttgatg gcaaaagtac caatctgacc accgaggtag tcaacgttga acaccagttc
      241 ctgcggcagc gcttcttcgt cacgccagat aacccagccg cagcccagcg gagccagacc
      301 gaatttatgg cctgaagcac tgatcgattt cacacgcggc aggcggaagt cccagacgat
      361 atc
//
LOCUS       DAJTNW010000345          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTNW010000345 DAJTNW010000000
VERSION     DAJTNW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.360"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>363
                     /locus_tag="OO179_004352"
     CDS             <1..>363
                     /locus_tag="OO179_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5534144.1"
                     /translation="LVLVLIPVVMTAIVGWHWFDAATYAANWNTADTTDGHAIIKSIL
                     LCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMA
                     ASGAPFAISASAILGNWAA"
BASE COUNT           66 a           81 c          100 g          116 t
ORIGIN      
        1 tctggtgctg gttcttattc ctgtggtgat gactgctatt gttggctggc attggtttga
       61 tgcggcaact tatgcagcta actggaatac tgcggatacc actgatggtc atgcgatcat
      121 taaaagtatt ctgctctgcc tgtgggcctt cgtgggtgtt gaatccgcag cagtaagtac
      181 tggtatggtt aaaaacccga aacgtaccgt tccgctggca accatgctgg gtactggttt
      241 agcaggtatt gtttatatcg ctgcgactca ggtgctttcc ggtatgtatc cgtcttctgt
      301 aatggcggct tccggtgctc cgtttgcaat cagtgcttca gctatcctcg gtaactgggc
      361 tgc
//
LOCUS       DAJTNW010000346          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTNW010000346 DAJTNW010000000
VERSION     DAJTNW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.361"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          110 a           76 c           63 g          106 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attat
//
LOCUS       DAJTNW010000347          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTNW010000347 DAJTNW010000000
VERSION     DAJTNW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.362"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..216
                     /locus_tag="OO179_004353"
     CDS             <1..216
                     /locus_tag="OO179_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5534145.1"
                     /translation="VYSDFWESVAGKTCGDWYKLPTQVVRKPLSNIASKKRSEYRKRY
                     ALLDYIHETAIRSLDAYPVNSEHQDLN"
BASE COUNT          115 a           61 c           69 g          108 t
ORIGIN      
        1 gtctacagtg atttctggga gtctgtagca gggaaaacct gtggtgactg gtacaaatta
       61 ccaacacagg tggttcgaaa gccgctaagc aatatagcta gtaaaaaacg ctctgagtat
      121 cgaaaacgat acgctttgct ggattatatc catgaaactg ctatccgctc tttggatgca
      181 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      241 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      301 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta cat
//
LOCUS       DAJTNW010000348          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTNW010000348 DAJTNW010000000
VERSION     DAJTNW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.363"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO179_004354"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO179_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5534146.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTNW010000349          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTNW010000349 DAJTNW010000000
VERSION     DAJTNW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.364"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT           84 a           70 c           89 g           99 t
ORIGIN      
        1 tgttagtctt tgcatgagca agcggactga cacatttctc tttgttctca ttcagaaaat
       61 ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga tacgttacgc tcctgatgac
      121 gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag ttagtgctat
      181 agctcaggaa caaattttcc caaattggga tatgctcgca aattgctggt gatgtgggga
      241 gaatctggtt gagttcggta gaattgattt ggaggcagaa cgcttaaatc gtggcgtcct
      301 gaaacgaaaa acggacctcc gtggaggtcc gttgatatga at
//
LOCUS       DAJTNW010000350          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTNW010000350 DAJTNW010000000
VERSION     DAJTNW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.366"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            31..318
                     /locus_tag="OO179_004355"
     CDS             31..318
                     /locus_tag="OO179_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR5534147.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT           97 a           71 c           86 g           80 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaa
//
LOCUS       DAJTNW010000351          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTNW010000351 DAJTNW010000000
VERSION     DAJTNW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.368"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(139..>312)
                     /locus_tag="OO179_004356"
     CDS             complement(139..>312)
                     /locus_tag="OO179_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCR5534148.1"
                     /translation="WQQWINKLPPARREDEDVKEIRWMIEELRVSYFAQQLGTPYPIS
                     DKRILQAMEQISG"
BASE COUNT           83 a           71 c           60 g           98 t
ORIGIN      
        1 gataaacatt attcatcttg cagctgagaa ttgtattttt ctgaattcct gaatttttgt
       61 ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat gcttaccggg ttttagattt
      121 attaggcaaa tagcagggtt aaccgctaat ctgctccatc gcctgcaaaa tacgcttatc
      181 tgaaatcgga taaggcgtac caagttgttg agcgaagtaa ctaacgcgca gctcttctat
      241 catccaacgg atctctttca cgtcttcatc ctcacgacgt gcaggcggca gtttgttgat
      301 ccattgctgc ca
//
LOCUS       DAJTNW010000352          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTNW010000352 DAJTNW010000000
VERSION     DAJTNW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.369"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..301
                     /locus_tag="OO179_004357"
     CDS             <1..301
                     /locus_tag="OO179_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5534149.1"
                     /translation="QIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLPVTLTPEP
                     DNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIREIKGSSH"
BASE COUNT           86 a           85 c           77 g           62 t
ORIGIN      
        1 gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc gccatcaata
       61 ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc ccgtgacgtt
      121 aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc agataaatac
      181 accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca ggcttaaagg
      241 taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga gcagccactg
      301 atgatatctt
//
LOCUS       DAJTNW010000353          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTNW010000353 DAJTNW010000000
VERSION     DAJTNW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.370"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..162
                     /locus_tag="OO179_004358"
     CDS             <1..162
                     /locus_tag="OO179_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5534150.1"
                     /translation="EDLLLGLISDEDREKLKAYRIYAKSLQAMDFSVITDKTSYNAIE
                     WPASPEISS"
     gene            186..>305
                     /locus_tag="OO179_004359"
     CDS             186..>305
                     /locus_tag="OO179_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5534151.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVAD"
BASE COUNT           83 a           71 c           74 g           77 t
ORIGIN      
        1 gaagacctgt tgctggggct aatcagcgac gaagaccggg aaaaactgaa agcgtaccgc
       61 atttacgcga aatcgctgca ggcgatggat ttcagtgtca tcactgataa aacctcatac
      121 aacgccattg aatggcccgc ttctccggaa atttcttcct gatttaattt atcgcgagaa
      181 aaactatgtc tgtagtgata tcaggtgcgc tgactgatgg cgcaggtatc tccatgtccg
      241 gataccatat tattctgaaa tcccgggtaa acaccccgga agtggtgatg cacactgttg
      301 ctgat
//
LOCUS       DAJTNW010000354          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTNW010000354 DAJTNW010000000
VERSION     DAJTNW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.371"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT          133 a           41 c           44 g           86 t
ORIGIN      
        1 cgattattca accgtgatca cattaaaaac aaaaatttcc accgaggaga aaaaataccc
       61 tcaaaaacca cctgaataat taaccattat tgttaaaaca acacgttaaa taatcacata
      121 acaaactcca ttaatcattg aaaatgagca cagaaataga aattaattat attcaattaa
      181 ttaaaatgtg tattgcttga tgtatttaaa aatattattt tcatggtgag agtggaggta
      241 aagaagacac aataataaaa aggaatgtga caaataatgg ttctggttgg gcgttaaatt
      301 taag
//
LOCUS       DAJTNW010000355          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTNW010000355 DAJTNW010000000
VERSION     DAJTNW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.372"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
BASE COUNT           90 a           92 c           51 g           71 t
ORIGIN      
        1 tgacctcctc agaataatcc ggcacgcgcc ggaggcattc gcacgcctga agcccgccag
       61 tgcacaaaaa atcagcgcta catacaaaaa acaacctcat catccacctt ctggtgcatc
      121 cggtcccccc tgtttttgat acaaaacacg cctcacagac ggggaatttt gcttatccac
      181 attaaactgc aagggacttc accataaggt tacaaccgtt cacgtcataa agcgccagac
      241 gtcaacccta cagggtgcaa tgtatctttt aaacacctgt ttatatctcc tttaaactac
      301 ttaa
//
LOCUS       DAJTNW010000356          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTNW010000356 DAJTNW010000000
VERSION     DAJTNW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.373"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..125
                     /gene="ubiK"
                     /locus_tag="OO179_004360"
     CDS             <1..125
                     /gene="ubiK"
                     /locus_tag="OO179_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5534152.1"
                     /translation="QVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT           98 a           61 c           49 g           92 t
ORIGIN      
        1 cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga atcagcgaac
       61 tggaaaatcg cagtactgag atcaaaaagc agccagatcc agagactctc cccccaacgc
      121 tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcctctaat ttacagtgtt
      181 ttctattaca ttatcgctta atcccattca gagtatcatc acaaaaatga atgattgcaa
      241 ttgaaaatat aattgttatg tattctttaa gttttactta atcgatattt agactggccc
//
LOCUS       DAJTNW010000357          298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTNW010000357 DAJTNW010000000
VERSION     DAJTNW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.denovo.374"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            58..>298
                     /locus_tag="OO179_004361"
     CDS             58..>298
                     /locus_tag="OO179_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5534153.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW"
BASE COUNT           86 a           73 c           75 g           64 t
ORIGIN      
        1 ccttgttccg tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg
       61 caaatgaaaa acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct
      121 atggctgata aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc
      181 aaacaggcgc tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa
      241 gccctggacg atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctggg
//
LOCUS       DAJTNW010000358         5390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.174, whole genome shotgun sequence.
ACCESSION   DAJTNW010000358 DAJTNW010000000
VERSION     DAJTNW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.174"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            69..284
                     /locus_tag="OO179_004362"
     CDS             69..284
                     /locus_tag="OO179_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5534154.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            277..663
                     /locus_tag="OO179_004363"
     CDS             277..663
                     /locus_tag="OO179_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5534155.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            671..1357
                     /locus_tag="OO179_004364"
     CDS             671..1357
                     /locus_tag="OO179_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5534156.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="OO179_004365"
     CDS             complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="OO179_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5534157.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2040..3245
                     /gene="tet(B)"
                     /locus_tag="OO179_004366"
     CDS             2040..3245
                     /gene="tet(B)"
                     /locus_tag="OO179_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5534158.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="OO179_004367"
     CDS             complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="OO179_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5534159.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4084..4455
                     /locus_tag="OO179_004368"
     CDS             4084..4455
                     /locus_tag="OO179_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5534160.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4465..>5390)
                     /locus_tag="OO179_004369"
     CDS             complement(4465..>5390)
                     /locus_tag="OO179_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5534161.1"
                     /translation="VDWSDIREQKRLIVLRASVALHGRSVTLYEKAFPLSEQCSKKAH
                     DQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENW
                     KPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPK
                     IYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTS
                     SSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRH
                     SGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1502 a          997 c         1157 g         1734 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg accactatta agtcatgtag
       61 ctggttttat ttcaattgaa cgttttcaaa gtctagctac agaaggaaaa atgttatcgc
      121 tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga
      181 aagctcaaca agaagggcga gagtcaatat ttgattttta caaaattagt attgctcata
      241 ttactcgcga atattcattt aaaaaggaca aggataatgt ttgatgttca cgttgtttta
      301 gataatcaaa taggacaatt agcattacta ggaaaaacat taggtaataa aggtattgga
      361 ttggaagggg gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa
      421 caaggaaagg aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg
      481 acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga
      541 gtattggcgg agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg
      601 atattaataa cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag
      661 tgaacttgcg atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc
      721 gataggggcg gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg
      781 gcgagcttgg acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag
      841 cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca
      901 tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat
      961 ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg
     1021 tacttgctat gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca
     1081 acagcaatgg atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca
     1141 tgaaatggga attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga
     1201 ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta
     1261 tgaatctaaa gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa
     1321 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1381 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1441 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1501 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1561 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1621 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     1681 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     1741 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     1801 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     1861 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     1921 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1981 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
     2041 tgaatagttc gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc
     2101 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
     2161 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
     2221 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
     2281 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
     2341 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
     2401 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
     2461 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
     2521 tttttatcgc tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg
     2581 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt
     2641 cggtatacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
     2701 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
     2761 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
     2821 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
     2881 gattcattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     2941 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     3001 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     3061 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     3121 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     3181 tgctatcgat gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg
     3241 cttagttatt tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttacg ataaagaaga tttagcttca aataaaacct atctatttta
     3361 tttatctttc aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc
     3421 ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa
     3481 gtttttaagc acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac
     3541 aatggcgttt accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc
     3601 aatgataggc atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc
     3661 aagataatgt tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt
     3721 tgtgatgtag gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt
     3781 tttattttta aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc
     3841 atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag
     3901 tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt
     3961 aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa
     4021 gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat
     4081 aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta
     4141 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
     4201 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
     4261 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
     4321 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga
     4381 tttaagtaca tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg
     4441 gaggcaatgc actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
     4501 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
     4561 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
     4621 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
     4681 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
     4741 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
     4801 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
     4861 taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg
     4921 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
     4981 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
     5041 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
     5101 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
     5161 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
     5221 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
     5281 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
     5341 tcgcaatact ataagtcgtt tttgctcacg aatatcagac cagtcaacaa
//
LOCUS       DAJTNW010000359         5390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.175, whole genome shotgun sequence.
ACCESSION   DAJTNW010000359 DAJTNW010000000
VERSION     DAJTNW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.175"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            69..284
                     /locus_tag="OO179_004370"
     CDS             69..284
                     /locus_tag="OO179_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5534162.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            277..663
                     /locus_tag="OO179_004371"
     CDS             277..663
                     /locus_tag="OO179_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5534163.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            671..1357
                     /locus_tag="OO179_004372"
     CDS             671..1357
                     /locus_tag="OO179_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5534164.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="OO179_004373"
     CDS             complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="OO179_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5534165.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2040..3245
                     /gene="tet(B)"
                     /locus_tag="OO179_004374"
     CDS             2040..3245
                     /gene="tet(B)"
                     /locus_tag="OO179_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5534166.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="OO179_004375"
     CDS             complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="OO179_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5534167.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4084..4455
                     /locus_tag="OO179_004376"
     CDS             4084..4455
                     /locus_tag="OO179_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5534168.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4465..>5390)
                     /locus_tag="OO179_004377"
     CDS             complement(4465..>5390)
                     /locus_tag="OO179_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5534169.1"
                     /translation="VDWSDIREQKRLIVLRASVALHGRSVTLYEKAFPLSEQCSKKAH
                     DQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENW
                     KPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPK
                     IYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTS
                     SSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRH
                     SGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1502 a          996 c         1157 g         1735 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg accactatta agtcatgtag
       61 ctggttttat ttcaattgaa cgttttcaaa gtctagctac agaaggaaaa atgttatcgc
      121 tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga
      181 aagctcaaca agaagggcga gagtcaatat ttgattttta caaaattagt attgctcata
      241 ttactcgcga atattcattt aaaaaggaca aggataatgt ttgatgttca cgttgtttta
      301 gataatcaaa taggacaatt agcattacta ggaaaaacat taggtaataa aggtattgga
      361 ttggaagggg gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa
      421 caaggaaagg aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg
      481 acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga
      541 gtattggcgg agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg
      601 atattaataa cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag
      661 tgaacttgcg atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc
      721 gataggggcg gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg
      781 gcgagcttgg acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag
      841 cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca
      901 tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat
      961 ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg
     1021 tacttgctat gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca
     1081 acagcaatgg atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca
     1141 tgaaatggga attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga
     1201 ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta
     1261 tgaatctaaa gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa
     1321 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1381 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1441 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1501 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1561 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1621 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     1681 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     1741 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     1801 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     1861 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     1921 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1981 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
     2041 tgaatagttc gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc
     2101 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
     2161 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
     2221 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
     2281 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
     2341 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
     2401 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
     2461 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
     2521 tttttatcgc tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg
     2581 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt
     2641 cggtatacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
     2701 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
     2761 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
     2821 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
     2881 gatttattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     2941 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     3001 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     3061 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     3121 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     3181 tgctatcgat gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg
     3241 cttagttatt tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttacg ataaagaaga tttagcttca aataaaacct atctatttta
     3361 tttatctttc aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc
     3421 ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa
     3481 gtttttaagc acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac
     3541 aatggcgttt accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc
     3601 aatgataggc atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc
     3661 aagataatgt tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt
     3721 tgtgatgtag gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt
     3781 tttattttta aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc
     3841 atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag
     3901 tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt
     3961 aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa
     4021 gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat
     4081 aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta
     4141 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
     4201 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
     4261 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
     4321 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga
     4381 tttaagtaca tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg
     4441 gaggcaatgc actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
     4501 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
     4561 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
     4621 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
     4681 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
     4741 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
     4801 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
     4861 taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg
     4921 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
     4981 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
     5041 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
     5101 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
     5161 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
     5221 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
     5281 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
     5341 tcgcaatact ataagtcgtt tttgctcacg aatatcagac cagtcaacaa
//
LOCUS       DAJTNW010000360         5258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.178, whole genome shotgun sequence.
ACCESSION   DAJTNW010000360 DAJTNW010000000
VERSION     DAJTNW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.178"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO179_004378"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO179_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5534170.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO179_004379"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO179_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5534171.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO179_004380"
     CDS             2399..3235
                     /locus_tag="OO179_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5534172.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO179_004381"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO179_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO179_004382"
     CDS             complement(3790..4179)
                     /locus_tag="OO179_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534173.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO179_004383"
     CDS             4397..4837
                     /locus_tag="OO179_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5534174.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1244 a         1410 c         1386 g         1218 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtaaa agtgatgtat aagtgatgta aaagtgat
//
LOCUS       DAJTNW010000361         5257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.179, whole genome shotgun sequence.
ACCESSION   DAJTNW010000361 DAJTNW010000000
VERSION     DAJTNW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.179"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO179_004384"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO179_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5534175.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1640..2398
                     /locus_tag="OO179_004385"
     CDS             1640..2398
                     /locus_tag="OO179_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5534176.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2398..3234
                     /locus_tag="OO179_004386"
     CDS             2398..3234
                     /locus_tag="OO179_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5534177.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3341..3745)
                     /locus_tag="OO179_004387"
                     /pseudo
     CDS             complement(3341..3745)
                     /locus_tag="OO179_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3789..4178)
                     /locus_tag="OO179_004388"
     CDS             complement(3789..4178)
                     /locus_tag="OO179_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534178.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4396..4836
                     /locus_tag="OO179_004389"
     CDS             4396..4836
                     /locus_tag="OO179_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5534179.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1244 a         1410 c         1386 g         1217 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat atttttttgg
     1621 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
     1681 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
     1741 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
     1801 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
     1861 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
     1921 tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat
     1981 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
     2041 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
     2101 gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa
     2161 cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg
     2221 aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc
     2281 agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc
     2341 ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg
     2401 ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac
     2461 accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag
     2521 ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg
     2581 cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa
     2641 tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc
     2701 gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct
     2761 gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa
     2821 aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc
     2881 aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc
     2941 agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc
     3001 gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt
     3061 gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac
     3121 caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga agaggagcaa
     3181 cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata ctagatatca
     3241 agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc gctggccaaa
     3301 gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa cgcagctgaa
     3361 acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca tcaaccagca
     3421 aggcggcact ttcggccatc cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac
     3481 agctgaaagc gaccaggtgc tcggcgtggc aagactcgca gcgaacccgt agaaagccat
     3541 gctccagccg cccgcattgg agaaattctt caaattcccg ttgcacatag cccggcaatt
     3601 cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca acgatctgat
     3661 agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga tcccggctgg
     3721 ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt ttacatacag
     3781 tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt tgctagacat
     3841 tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag gtacatgtta
     3901 ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc gacagaatta
     3961 atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc aaaggatgca
     4021 gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt aaaactacct
     4081 cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc catgtgacca
     4141 aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc tttgactttt
     4201 aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg aaatactacg
     4261 cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc
     4321 tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt acacacagga
     4381 gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt aacaggaaag
     4441 tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg cacaggaaaa
     4501 aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga gctgcgcact
     4561 tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag ccaggcggaa
     4621 aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac tgttacccta
     4681 atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa
     4741 caactacagg ccgaaattag ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga
     4801 ttctggtcgc ggctgtttcg ctccggttca gagtaacagt gcaggtgact ctgatttacc
     4861 ataacatctg ttatcagcac cgcctgagag gccctcaggg cgtttctgac gggaaaccgg
     4921 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     4981 catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca gccggggtaa
     5041 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
     5101 ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc aggaaggggg
     5161 tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag
     5221 tgatgtaaaa gtgatgtata agtgatgtaa aagtgat
//
LOCUS       DAJTNW010000362         4212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.199, whole genome shotgun sequence.
ACCESSION   DAJTNW010000362 DAJTNW010000000
VERSION     DAJTNW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.199"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..489
                     /locus_tag="OO179_004390"
     CDS             <1..489
                     /locus_tag="OO179_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR5534180.1"
                     /translation="VNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQ
                     ERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTE
                     RDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKA
                     NG"
     gene            482..1216
                     /gene="frdB"
                     /locus_tag="OO179_004391"
     CDS             482..1216
                     /gene="frdB"
                     /locus_tag="OO179_004391"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5534181.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1227..1622
                     /gene="frdC"
                     /locus_tag="OO179_004392"
     CDS             1227..1622
                     /gene="frdC"
                     /locus_tag="OO179_004392"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5534182.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1633..1992
                     /gene="frdD"
                     /locus_tag="OO179_004393"
     CDS             1633..1992
                     /gene="frdD"
                     /locus_tag="OO179_004393"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5534183.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2055..3188
                     /locus_tag="OO179_004394"
     CDS             2055..3188
                     /locus_tag="OO179_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5534184.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3277..3810
                     /gene="blc"
                     /locus_tag="OO179_004395"
     CDS             3277..3810
                     /gene="blc"
                     /locus_tag="OO179_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5534185.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3807..4124)
                     /gene="sugE"
                     /locus_tag="OO179_004396"
     CDS             complement(3807..4124)
                     /gene="sugE"
                     /locus_tag="OO179_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5534186.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1039 a         1108 c         1109 g          956 t
ORIGIN      
        1 gttaaccagg atggcggcga aaactgggcg aagatccgcg acgaaatggg cctggcaatg
       61 gaagaaggtt gcggtatcta ccgtacgccg gaactgatgc agaaaaccat cgacaagctg
      121 gcagagctgc aggaacgctt caagcgcgtg cgcatcaccg acacctccag cgtgttcaac
      181 accgacctgc tctacaccat tgaactgagc cacggtctga acgttgctga atgtatggcg
      241 cactccgcaa tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc
      301 accgagcgtg acgacgtcaa ctttctcaaa cacaccctcg ccttccgcga tgctgatggc
      361 acgactcgcc tggagtacag cgacgtgaag attactacgc tgccgccagc taaacgcgtt
      421 tacggtggcg aagcggatgc agccgataag gcggaagcag ccaataataa ggagaaggcg
      481 aatggctgag atgaaaaacc tgaaaattga ggtggtgcgc tataacccgg aagtcgatac
      541 cgcaccgcat agcgcattct atgaagtgcc ttatgacgca actacctcat tactggatgc
      601 gctgggctac atcaaagaca acctggcacc ggacctgagt taccgctggt cctgccgtat
      661 ggcgatttgt ggttcctgcg gcatgatggt taacaacgtg ccaaaactgg catgtaaaac
      721 cttcctgcgt gattacaccg acggtatgaa ggttgaagcg ttagctaact tcccgattga
      781 acgcgatctg gtggtcgata tgacccactt catcgaaagt ctggaagcga tcaaaccgta
      841 catcatcggc aactcccgca ccgcggatca gggtactaac atccagaccc cggcgcagat
      901 ggcaaagtat caccagttct ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc
      961 gcagtttggc ctgaacccag agttcatcgg tccggctgcc attacgctgg cgcatcgtta
     1021 taacgaagat agccgcgacc acggtaagaa ggagcgtatg gcgcagttga acagccagaa
     1081 cggtgtatgg agctgtactt tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga
     1141 tccggctgcg gccattcagc agggcaaagt agaaagttcg aaagactttc ttatcgcgac
     1201 cctgaaacca cgctaaggag tgcaacatga cgactaaacg taaaccgtat gtacggccaa
     1261 tgacgtccac ctggtggaaa aaattgccgt tttatcgctt ttacatgctg cgcgaaggca
     1321 cggcggttcc ggctgtgtgg ttcagcattg aactgatttt cgggctgttt gccctgaaaa
     1381 atggcccgga agcctgggcg ggattcgtcg actttttaca aaacccggtt atcgtgatca
     1441 ttaacctgat cactctgacg gcagctctgc tgcacaccaa aacctggttt gaactggcac
     1501 cgaaagcggc caatatcatt gtaaaagacg aaaaaatggg accagagcca attatcaaaa
     1561 gtctctgggc ggtaactgtg gttgccacca tcgtaatcct gtttgttgcc ctgtactggt
     1621 aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc
     1681 ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg
     1741 ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg
     1801 gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg
     1861 tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaatcca cgtgcctgcg
     1921 ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtgtc
     1981 gttacaatct aacgcatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
     2041 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttact
     2101 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
     2161 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
     2221 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
     2281 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc
     2341 gaggggaaat caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac
     2401 agtggaatgg gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc
     2461 aggtgccgga tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
     2521 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
     2581 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
     2641 agccactcaa actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg
     2701 cctggggata tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag
     2761 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc
     2821 cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
     2881 ggcaaaccgg cgatatgtac cagggcctgg gctgggaaat gctggactgg ccggtcaatc
     2941 ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag
     3001 cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg
     3061 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     3121 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc
     3181 tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc
     3241 atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc
     3301 gcagcgacag ctgcatttct ggtcgttgcc tgtagttttc ctacgccgcc gcgtggcgtg
     3361 accgtagtga ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt
     3421 tttgatcacc gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat
     3481 gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag
     3541 agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc
     3601 tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg
     3661 ctggtttgcg gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct
     3721 gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag
     3781 tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc
     3841 aatacgatta acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca
     3901 atgccggtga tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct
     3961 accggtaacg atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg
     4021 gtaataacac tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat
     4081 accacttcca gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat
     4141 ttggggccgt ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga
     4201 acggatattt tg
//
LOCUS       DAJTNW010000363         4212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.200, whole genome shotgun sequence.
ACCESSION   DAJTNW010000363 DAJTNW010000000
VERSION     DAJTNW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.200"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            <1..489
                     /locus_tag="OO179_004397"
     CDS             <1..489
                     /locus_tag="OO179_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR5534187.1"
                     /translation="VNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQ
                     ERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTE
                     RDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKA
                     NG"
     gene            482..1216
                     /gene="frdB"
                     /locus_tag="OO179_004398"
     CDS             482..1216
                     /gene="frdB"
                     /locus_tag="OO179_004398"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5534188.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1227..1622
                     /gene="frdC"
                     /locus_tag="OO179_004399"
     CDS             1227..1622
                     /gene="frdC"
                     /locus_tag="OO179_004399"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5534189.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1633..1992
                     /gene="frdD"
                     /locus_tag="OO179_004400"
     CDS             1633..1992
                     /gene="frdD"
                     /locus_tag="OO179_004400"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5534190.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2055..3188
                     /locus_tag="OO179_004401"
     CDS             2055..3188
                     /locus_tag="OO179_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5534191.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3277..3810
                     /gene="blc"
                     /locus_tag="OO179_004402"
     CDS             3277..3810
                     /gene="blc"
                     /locus_tag="OO179_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5534192.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3807..4124)
                     /gene="sugE"
                     /locus_tag="OO179_004403"
     CDS             complement(3807..4124)
                     /gene="sugE"
                     /locus_tag="OO179_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5534193.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1039 a         1107 c         1109 g          957 t
ORIGIN      
        1 gttaaccagg atggcggcga aaactgggcg aagatccgcg acgaaatggg cctggcaatg
       61 gaagaaggtt gcggtatcta ccgtacgccg gaactgatgc agaaaaccat cgacaagctg
      121 gcagagctgc aggaacgctt caagcgcgtg cgcatcaccg acacctccag cgtgttcaac
      181 accgacctgc tctacaccat tgaactgagc cacggtctga acgttgctga atgtatggcg
      241 cactccgcaa tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc
      301 accgagcgtg acgacgtcaa ctttctcaaa cacaccctcg ccttccgcga tgctgatggc
      361 acgactcgcc tggagtacag cgacgtgaag attactacgc tgccgccagc taaacgcgtt
      421 tacggtggcg aagcggatgc agccgataag gcggaagcag ccaataataa ggagaaggcg
      481 aatggctgag atgaaaaacc tgaaaattga ggtggtgcgc tataacccgg aagtcgatac
      541 cgcaccgcat agcgcattct atgaagtgcc ttatgacgca actacctcat tactggatgc
      601 gctgggctac atcaaagaca acctggcacc ggacctgagt taccgctggt cctgccgtat
      661 ggcgatttgt ggttcctgcg gcatgatggt taacaacgtg ccaaaactgg catgtaaaac
      721 cttcctgcgt gattacaccg acggtatgaa ggttgaagcg ttagctaact tcccgattga
      781 acgcgatctg gtggtcgata tgacccactt catcgaaagt ctggaagcga tcaaaccgta
      841 catcatcggc aactcccgca ccgcggatca gggtactaac atccagaccc cggcgcagat
      901 ggcaaagtat caccagttct ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc
      961 gcagtttggc ctgaacccag agttcatcgg tccggctgcc attacgctgg cgcatcgtta
     1021 taacgaagat agccgcgacc acggtaagaa ggagcgtatg gcgcagttga acagccagaa
     1081 cggtgtatgg agctgtactt tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga
     1141 tccggctgcg gccattcagc agggcaaagt agaaagttcg aaagactttc ttatcgcgac
     1201 cctgaaacca cgctaaggag tgcaacatga cgactaaacg taaaccgtat gtacggccaa
     1261 tgacgtccac ctggtggaaa aaattgccgt tttatcgctt ttacatgctg cgcgaaggca
     1321 cggcggttcc ggctgtgtgg ttcagcattg aactgatttt cgggctgttt gccctgaaaa
     1381 atggcccgga agcctgggcg ggattcgtcg actttttaca aaacccggtt atcgtgatca
     1441 ttaacctgat cactctgacg gcagctctgc tgcacaccaa aacctggttt gaactggcac
     1501 cgaaagcggc caatatcatt gtaaaagacg aaaaaatggg accagagcca attatcaaaa
     1561 gtctctgggc ggtaactgtg gttgccacca tcgtaatcct gtttgttgcc ctgtactggt
     1621 aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc
     1681 ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg
     1741 ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg
     1801 gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg
     1861 tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaatcca cgtgcctgcg
     1921 ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtgtc
     1981 gttacaatct aacgcatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
     2041 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttact
     2101 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
     2161 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
     2221 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
     2281 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc
     2341 gaggggaaat caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac
     2401 agtggaatgg gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc
     2461 aggtgccgga tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
     2521 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
     2581 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
     2641 agccactcaa actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg
     2701 cctggggata tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag
     2761 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc
     2821 cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
     2881 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc
     2941 ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag
     3001 cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg
     3061 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     3121 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc
     3181 tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc
     3241 atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc
     3301 gcagcgacag ctgcatttct ggtcgttgcc tgtagttttc ctacgccgcc gcgtggcgtg
     3361 accgtagtga ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt
     3421 tttgatcacc gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat
     3481 gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag
     3541 agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc
     3601 tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg
     3661 ctggtttgcg gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct
     3721 gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag
     3781 tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc
     3841 aatacgatta acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca
     3901 atgccggtga tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct
     3961 accggtaacg atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg
     4021 gtaataacac tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat
     4081 accacttcca gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat
     4141 ttggggccgt ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga
     4201 acggatattt tg
//
LOCUS       DAJTNW010000364         3340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.215, whole genome shotgun sequence.
ACCESSION   DAJTNW010000364 DAJTNW010000000
VERSION     DAJTNW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.215"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            1..308
                     /gene="prfH"
                     /locus_tag="OO179_004404"
                     /pseudo
     CDS             1..308
                     /gene="prfH"
                     /locus_tag="OO179_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(365..1822)
                     /gene="pepD"
                     /locus_tag="OO179_004405"
     CDS             complement(365..1822)
                     /gene="pepD"
                     /locus_tag="OO179_004405"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5534194.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2084..2542
                     /gene="gpt"
                     /locus_tag="OO179_004406"
     CDS             2084..2542
                     /gene="gpt"
                     /locus_tag="OO179_004406"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5534195.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2634..>3340
                     /locus_tag="OO179_004407"
     CDS             2634..>3340
                     /locus_tag="OO179_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5534196.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFS"
BASE COUNT          797 a          857 c          873 g          813 t
ORIGIN      
        1 aggagcaatc cgatgcgatc cggtatgaga cgctgcgttc gtcggggccg ggcggtcaac
       61 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
      121 aggttcagtc agagcgcagc cagcatgcta acaaacggct ggcgcgattg ctgattgcct
      181 ggaagctgga tcagcagcaa taggaaaata gcgcggtgct gaaatcacag cggcgaatgt
      241 tccatcacca gattgaacgt ggcaacccgc gacgaacgtt tacagggatg gcttttatcg
      301 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
      361 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta
      421 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
      481 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
      541 taatctggat gttcggcgtc ttgttgaaca ggcgctgata ggtttcacgt accagatgca
      601 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
      661 cgccagccag tttacccagc gaatccagca tgctcaccac gtagtcttta ccgctgtcga
      721 tcagtgaacg gatcaggcag tgaatttcta cgttattgtc agtcatggtc accacaccga
      781 cgttcaggga ggtttcgacc acaccttttg ccacgtcgga attgcggatc acaccgttcg
      841 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
      901 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
      961 tcaggatctc ctgataggta ttcaccagag atttcagtgc gtcgacttta tcagctgcga
     1021 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     1081 cgataaggcg caggtccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
     1141 tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta
     1201 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     1261 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     1321 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     1381 cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     1441 ccagaaccgc cagtgcagag gccataccaa tgccgttatc cgcacccagc gtggtgccgc
     1501 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     1561 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     1621 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     1681 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     1741 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     1801 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     1861 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtatt
     1921 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1981 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     2041 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     2101 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     2161 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gggcgttact
     2221 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     2281 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     2341 tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa tgtatccaaa
     2401 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     2461 tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt
     2521 cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg ttgttctttt
     2581 taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     2641 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     2701 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcagctctt gatggtaaaa
     2761 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc
     2821 cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg
     2881 atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat gagtgggcca
     2941 cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgaaa ctcagtggtc
     3001 gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag
     3061 atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc
     3121 gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg
     3181 gctttttgca tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc
     3241 tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca
     3301 ttgcgatgct gactattgat atgccgtcgg tgggcttttc
//
LOCUS       DAJTNW010000365         2252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.232, whole genome shotgun sequence.
ACCESSION   DAJTNW010000365 DAJTNW010000000
VERSION     DAJTNW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.232"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            251..724
                     /locus_tag="OO179_004408"
     CDS             251..724
                     /locus_tag="OO179_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5534197.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            819..1343
                     /gene="sat2"
                     /locus_tag="OO179_004409"
     CDS             819..1343
                     /gene="sat2"
                     /locus_tag="OO179_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5534198.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1401..2192
                     /gene="aadA1"
                     /locus_tag="OO179_004410"
     CDS             1401..2192
                     /gene="aadA1"
                     /locus_tag="OO179_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5534199.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          632 a          503 c          557 g          560 t
ORIGIN      
        1 agagatgctt gtttaccggt agagttttaa tttaatgcta aataaattaa aatgttatga
       61 gttctttggg tgagataatg tgcatcgtgc aagcaggata gacggcatgc acgatttgta
      121 ataacagagt gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat tatattaatt
      181 aacggtaagc atcagcgggt gacaaaacga gcatgcttac taataaaatg ttaacctctg
      241 aggaagaatt gtgaaactat cactaatggt agctatatcg aagaatggag ttatcgggaa
      301 tggccctgat attccatgga gtgccaaagg tgaacagctc ctgtttaaag ctattaccta
      361 taaccaatgg ctgttggttg gacgcaagac ttttgaatca atgggagcat tacccaaccg
      421 aaagtatgcg gtcgtaacac gttcaagttt tacatctgac aatgagaacg tattgatctt
      481 tccatcaatt aaagatgctt taaccaacct aaagaaaata acggatcatg tcattgtttc
      541 aggtggtggg gagatataca aaagcctgat cgatcaagta gatacactac atatatctac
      601 aatagacatc gagccggaag gtgatgttta ctttcctgaa atccccagca attttaggcc
      661 agtttttacc caagacttcg cctctaacat aaattatagt taccaaatct ggcaaaaggg
      721 ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc ttttgtacca aaagccgcgc
      781 caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg
      841 agcaggtggc ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcgatg
      901 tgcacctatc cgaccaaggc tttgaactat ctaccagaag tgtgagcccc taccggaagg
      961 attacatctc ggatgatgac tctgatgaag actctgcttg ctatggcgca ttcatcgacc
     1021 aagagcttgt cgggaagatt gaactcaact caacatggaa cgatctagcc tctatcgaac
     1081 acattgttgt gtcgcacacg caccgaggca aaggagtcgc gcacagtctc atcgaatttg
     1141 cgaaaaagtg ggcactaagc agacagctcc ttggcatacg attagagaca caaacgaaca
     1201 atgtacctgc ctgcaatttg tacgcaaaat gtggctttac tctcggcggc attgacctgt
     1261 tcacgtataa aactagacct caagtctcga acgaaacagc gatgtactgg tactggttct
     1321 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct
     1381 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     1441 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1501 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1561 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1621 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     1681 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     1741 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     1801 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     1861 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     1921 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     1981 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2041 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2101 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     2161 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     2221 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJTNW010000366         2249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.233, whole genome shotgun sequence.
ACCESSION   DAJTNW010000366 DAJTNW010000000
VERSION     DAJTNW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.233"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            251..724
                     /locus_tag="OO179_004411"
     CDS             251..724
                     /locus_tag="OO179_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5534200.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            819..1343
                     /gene="sat2"
                     /locus_tag="OO179_004412"
     CDS             819..1343
                     /gene="sat2"
                     /locus_tag="OO179_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5534201.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1401..2189
                     /gene="aadA1"
                     /locus_tag="OO179_004413"
     CDS             1401..2189
                     /gene="aadA1"
                     /locus_tag="OO179_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5534202.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          629 a          503 c          557 g          560 t
ORIGIN      
        1 agagatgctt gtttaccggt agagttttaa tttaatgcta aataaattaa aatgttatga
       61 gttctttggg tgagataatg tgcatcgtgc aagcaggata gacggcatgc acgatttgta
      121 ataacagagt gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat tatattaatt
      181 aacggtaagc atcagcgggt gacaaaacga gcatgcttac taataaaatg ttaacctctg
      241 aggaagaatt gtgaaactat cactaatggt agctatatcg aagaatggag ttatcgggaa
      301 tggccctgat attccatgga gtgccaaagg tgaacagctc ctgtttaaag ctattaccta
      361 taaccaatgg ctgttggttg gacgcaagac ttttgaatca atgggagcat tacccaaccg
      421 aaagtatgcg gtcgtaacac gttcaagttt tacatctgac aatgagaacg tattgatctt
      481 tccatcaatt aaagatgctt taaccaacct aaagaaaata acggatcatg tcattgtttc
      541 aggtggtggg gagatataca aaagcctgat cgatcaagta gatacactac atatatctac
      601 aatagacatc gagccggaag gtgatgttta ctttcctgaa atccccagca attttaggcc
      661 agtttttacc caagacttcg cctctaacat aaattatagt taccaaatct ggcaaaaggg
      721 ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc ttttgtacca aaagccgcgc
      781 caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg
      841 agcaggtggc ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcgatg
      901 tgcacctatc cgaccaaggc tttgaactat ctaccagaag tgtgagcccc taccggaagg
      961 attacatctc ggatgatgac tctgatgaag actctgcttg ctatggcgca ttcatcgacc
     1021 aagagcttgt cgggaagatt gaactcaact caacatggaa cgatctagcc tctatcgaac
     1081 acattgttgt gtcgcacacg caccgaggca aaggagtcgc gcacagtctc atcgaatttg
     1141 cgaaaaagtg ggcactaagc agacagctcc ttggcatacg attagagaca caaacgaaca
     1201 atgtacctgc ctgcaatttg tacgcaaaat gtggctttac tctcggcggc attgacctgt
     1261 tcacgtataa aactagacct caagtctcga acgaaacagc gatgtactgg tactggttct
     1321 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct
     1381 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     1441 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1501 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1561 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1621 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     1681 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     1741 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     1801 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     1861 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     1921 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     1981 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2041 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     2101 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     2161 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     2221 tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DAJTNW010000367         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.234, whole genome shotgun sequence.
ACCESSION   DAJTNW010000367 DAJTNW010000000
VERSION     DAJTNW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.234"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..211)
                     /locus_tag="OO179_004414"
     CDS             complement(<1..211)
                     /locus_tag="OO179_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCR5534203.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLAN"
     gene            421..1251
                     /locus_tag="OO179_004415"
     CDS             421..1251
                     /locus_tag="OO179_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5534204.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1364..2155
                     /gene="aadA1"
                     /locus_tag="OO179_004416"
     CDS             1364..2155
                     /gene="aadA1"
                     /locus_tag="OO179_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5534205.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          666 a          501 c          516 g          532 t
ORIGIN      
        1 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
       61 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      121 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      181 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      241 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      301 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttgggcgaac
      361 ccggagcctc attaattgtt agccgttaaa attaagccct ttaccaaacc aatacttatt
      421 atgaaaaaca caatacatat caacttcgct atttttttaa taattgcaaa tattatctac
      481 agcagcgcca gtgcatcaac agatatctct actgttgcat ctccattatt tgaaggaact
      541 gaaggttgtt ttttacttta cgatgcatcc acaaacgctg aaattgctca attcaataaa
      601 gcaaagtgtg caacgcaaat ggcaccagat tcaactttca agatcgcatt atcacttatg
      661 gcatttgatg cggaaataat agatcagaaa accatattca aatgggataa aacccccaaa
      721 ggaatggaga tctggaacag caatcataca ccaaagacgt ggatgcaatt ttctgttgtt
      781 tgggtttcgc aagaaataac ccaaaaaatt ggattaaata aaatcaagaa ttatctcaaa
      841 gattttgatt atggaaatca agacttctct ggagataaag aaagaaacaa cggattaaca
      901 gaagcatggc tcgaaagtag cttaaaaatt tcaccagaag aacaaattca attcctgcgt
      961 aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac catagagaac
     1021 atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg tgcaggattc
     1081 acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag caaatcagga
     1141 cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt aacatcaagc
     1201 ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata aaaaatctaa
     1261 tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc ttttggctcc
     1321 cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg aagtggtgat
     1381 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1441 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1501 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1561 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1621 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1681 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1741 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1801 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1861 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1921 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1981 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2041 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2101 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     2161 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTNW010000368         2212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.235, whole genome shotgun sequence.
ACCESSION   DAJTNW010000368 DAJTNW010000000
VERSION     DAJTNW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.235"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            complement(<1..211)
                     /locus_tag="OO179_004417"
     CDS             complement(<1..211)
                     /locus_tag="OO179_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCR5534206.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLAN"
     gene            421..1251
                     /locus_tag="OO179_004418"
     CDS             421..1251
                     /locus_tag="OO179_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5534207.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1364..2152
                     /gene="aadA1"
                     /locus_tag="OO179_004419"
     CDS             1364..2152
                     /gene="aadA1"
                     /locus_tag="OO179_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5534208.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          663 a          501 c          516 g          532 t
ORIGIN      
        1 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
       61 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      121 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      181 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      241 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      301 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttgggcgaac
      361 ccggagcctc attaattgtt agccgttaaa attaagccct ttaccaaacc aatacttatt
      421 atgaaaaaca caatacatat caacttcgct atttttttaa taattgcaaa tattatctac
      481 agcagcgcca gtgcatcaac agatatctct actgttgcat ctccattatt tgaaggaact
      541 gaaggttgtt ttttacttta cgatgcatcc acaaacgctg aaattgctca attcaataaa
      601 gcaaagtgtg caacgcaaat ggcaccagat tcaactttca agatcgcatt atcacttatg
      661 gcatttgatg cggaaataat agatcagaaa accatattca aatgggataa aacccccaaa
      721 ggaatggaga tctggaacag caatcataca ccaaagacgt ggatgcaatt ttctgttgtt
      781 tgggtttcgc aagaaataac ccaaaaaatt ggattaaata aaatcaagaa ttatctcaaa
      841 gattttgatt atggaaatca agacttctct ggagataaag aaagaaacaa cggattaaca
      901 gaagcatggc tcgaaagtag cttaaaaatt tcaccagaag aacaaattca attcctgcgt
      961 aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac catagagaac
     1021 atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg tgcaggattc
     1081 acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag caaatcagga
     1141 cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt aacatcaagc
     1201 ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata aaaaatctaa
     1261 tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc ttttggctcc
     1321 cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg aagtggtgat
     1381 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1441 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1501 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1561 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1621 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1681 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1741 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1801 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1861 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1921 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1981 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2041 agctaggcag gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     2101 agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     2161 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJTNW010000369          953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401674
            SAMEA8900036-rid15821683.guided.282, whole genome shotgun sequence.
ACCESSION   DAJTNW010000369 DAJTNW010000000
VERSION     DAJTNW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900036
            Sequence Read Archive: ERR6005271
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478185.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:10:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,420
            CDSs (total)                      :: 4,342
            Genes (coding)                    :: 3,981
            CDSs (with protein)               :: 3,981
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 163 of 361
            Pseudo Genes (incomplete)         :: 94 of 361
            Pseudo Genes (internal stop)      :: 145 of 361
            Pseudo Genes (multiple problems)  :: 38 of 361
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900036-rid15821683.guided.282"
                     /serovar="S. flexneri 1b"
                     /isolate="401674"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-19"
     gene            264..923
                     /locus_tag="OO179_004420"
     CDS             264..923
                     /locus_tag="OO179_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5534209.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          264 a          207 c          215 g          267 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taa
//
